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RISTOPATHOLOGY
(Subacute Oral)
GENERAL DESCRIPTION OF TEST
Six male rats were given ten successive daily intubated oral doses of 2000 mg/kg of a 20% suspension of lead titanate catalyst in peanut oil. Tissues from three of the rats were collected at the end of the twelve day test period. Tissues from the other three rats were collected after a fourteen day recovery period.
An equal number of male rats served as controls for each of the experi mental procedures.
SUMMARY OF RESULTS
1. All rats survived the trials.
2. There was a significant decrease in the amount of perinuclear frothy hepatocellular vacuolation and in the amount of globular hepatocellular vacuolation in the livers of test rats. This was most marked in the livers of the three rats of the test phase.
In addition, there was a decrease in the actual weights of livers of test animals (Table II) and relative weights of livers of test animals (Table III). This likewise was most marked in the three rats of the test phase and was consistent with the decrease in hepatocellular vacuolation.
The livers of recovery phase rats occupied an intermediate position between test phase rats and control rats as regards both the histologic findings and weights.
3. A severe bilateral exudative pleuritis was present in R 56825, Particles of the experimental substance were evident in the pleural exudate. In addition, there was a large hematoma In one lung, peritonitis of the splenic omentum and degeneration of spermatic tubules with giant cell formation.
4. Manifestations of intercurrent disease-processes were observed in specimens from both test and control animals. No significant differences could be discerned between test and control groups as regards intercurrent lesions.
. /;2 7
DUP040000955
H 3.437 page 2
DISCUSSION
A detailed summary of the histologic observations are presented in Table I. The lesions of murine pneumonitis, hydronephrosis, focal nephritis, and hepatic microgranulomata were interpreted as the result of intercurrent disease processes.
In R 56825, the pulmonary hematoma and pleuritis were interpreted as the result of trauma during intubation. The esophagus was probably ruptured and the material, in part, deposited in the thoracic cavity. The peritonitis was interpreted as an extension (direct or hematogenous) of the pleural inflammation. The testicular degeneration was interpreted as being related to the general de bilitated state of the animal which in turn was the result of both the experimental procedure and the experimental accident.
Liver: The histologic changes observed in the livers of test animals were consistent with marasmus. This is substantiated by both the marked losses in total body weight and organ weight. The lesions were in the process of being reversed during the recovery phase.
The changes seen in the livers were an effect of the experimental procedures which for one reason or another caused a conditioned malnutrition, i.e. interference With intake, absorption, assimulation, etc.
Other Organs: No lesions were observed in the other organs examined (and listed in Table I) that could be attributed to the experimental procedures.
CONCLUSIONS:
1. The experimental procedures produced histologic changes in the livers of test animals which Were consistent with marasmus,
2. The hepatic changes were largely reversed during the recovery phase and appeared to be completely reversible.
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NECROPSY RECORD
Material
Animal ,A
Strain !^Ajf~iG Sex.jxfL. Age
Fvriplo^t
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Method
Survival: dh
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Condition
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GROSS PATHOLOGY
Unless indicated no abnormality detected
Histology Recommended: Ym.
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Tissue Saved:
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Thor./Abd. Br./Sp.C Ht./B.V. Tr./La./Br. Lungs L.N. Liver/G.B. Spleen Pancreas
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Date 3-f-Lj
ER-8420
WHITE--PATHOLOGY, FILE
BLUE--TOXICOLOGY, MICRO
Pathologist. Date _
PINK-TOXICOLOGY, GROSS
"DUP040000960
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NECROPSY RECORD
Material
Animal A/j l j A
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Pathologist. Date
PINK-TOXICOLOGY, GROSS
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_______________ _______________ ____________________________________ Date
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ER.8420
WHITE--PATHOLOGY, FILE
BLUE-TOXICOLOGY, MOO
Pathologist. Date.
PINKr-TOXICOLOGY, GROSS
DUP040000962
NECROPSY RECORD
Material
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Cause of Death: HISTOPATHOLOGY
ER.8420
WHITE-PATHOLOGY, FILE
BLUE-TOXICOLOGY, MICRO
Pathologist. Date.
PINK-TOXICOLOGY, GROSS
"D U P040000963
NECROPSY RECORD
Material
Animal Aacb
Strain Qjue-eaQ Sex.j
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Cause of Death: HISTOPATHOLOGY
AU% Pathologist -. . ...... . ..
Date ^
Pathologist,
Date
DUP040000964
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WHITE--PATHOLOGY, FILE
BLUE--TOXICOLOGY, MICRO
PINK-TOXICOLOGY, GROSS
NECROPSY RECORD
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Animal ,,A.i3l & . ,__Strain @V&- C$ Sex-d-L--Age
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SX JJ*'*
GROSS PATHOLOGY
Unieis indicated no abnormality detected
Histology Recommended: Yes_____No
Tissue Saved;
Yes No
Histology Follows:
V** Klii
Organ
Wt.
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Micro
Organ
Wt.
Gross
Micro
Organ
Wt.
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Micro
Thor./Abd. Br./SpX. Ht./B.V. Tr./La./Br. Lungs L.N. Liver/G.B. Spleen Pancreas
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Pathologist. Date_ -/C-C3
Pathologist.
Date:
DUP040000965
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WHITE--PATHOLOGY, FILE
BLUE-TOXICOLOGY, MICRO
PINK-TOXICOLOGY, GROSS