Document 5bxJ0DjYwq6gVakL0Yoz2OG4
MICROBICS MICROTOX@ TEST SUBSTANCE
TOXICITY TEST
Identity:A mixturecontainingperfluorooctanesulfonatmea;y also be referredto as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfoniacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 101. The testsample isFC206CF. Currentinformationindicatesitisa mixtureof0.5% PFOS, 10% diethyleneglycolmonobutyl ether,78.08% water,1.375% acrylicfoamer, 1.5% sodium octylsulfate,1% sulfonatedalkylethersalts,6.0% urea,0.5% triethanolaminea,nd 0.05% tolyltriazole.
The followingsummary appliesto a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotox@ "BASIC" Procedure
Test type: Acute static
GLP: No
Year Completed: 1991
Species: Photobacteriumphosphoreum
Analyticalmonitoring: pH and osmoticcondition.
Exposure period:30-minutes
Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@
Test organism source: Microbics'Reagent.
Test conditions:
Diluent: MilliporMeilli-QTM water
Test temperature: Not noted.
Stock and testsolution preparation: A primary2000 mg/L stock
solutionwas prepared by dissolving200 mg oftestsubstance in 100
Milli_QTM
mL of Millipore
water. The pH ofthe stocksolutionwas 6.5,
and an osmotic adjustmentwas made using200 mg NaCi dissolvedin
111 0 mL stocksolution.Appearance oftestsolutionswas noted as clear,colorless"A.lltestsolutionswere made by proportional dilutions.
Exposure vessels: 4 mL cuveftes.
Number of replicates:two
Number of concentrations: Four plusa blankcontrol
Element basis: Percent lightloss
RESULTS
NominalconcentrationBsl:ankcontro1l2,5,250,500,and 1000 mg/L
Element value:
5-minute EC,50= >1000 mg/L 15-minute EC50 = 890 (730 - 1000) mg/L 30-minute EC50 = 680 (610 - 750) mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesr(-porteadpplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 30-minute EC50 forPhotobacterium phosphoreum was
determined to be 680 mg/L witha 95% confidenceintervaolf 610 to 750 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
Reliability:Klimischranking= 2. The study meets allcriterifaorquality testing.However, the sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportion inthe solution.
REFERENCES
This studywas conducted by the 3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number J1884-4,1991.
OTHER
Last changed: 6/28/00.
ENVIRONMENRAL LABoRATORy L41CROSICS MICRCrrOX0 SYSTEM
RAW DATA
LR
Pg of
TEST SUBSTANCE:
TEST DATE: 711 -y q I
ANALYST:
SAMPLE CUVERTE
t3i
MA )4 FINAL-XSSAY CONCENTRATK)N
BLAWCONTFIOL
"UCATE
IA5-
ILI P)
/of
INITIAL FEADRYG
1 (0)
'7,3 107
1/0 /17
5-MIN 1 (5)
60 73
FINAL READINGS 15-MIN 1 (15)
30-MIN 1(30)
q7
51
56
109 591, 70
ClIs 51
115-
-q, 3 7
35 30
I>vpli'czL4e
39
Ptock soin. pr*p:
7 1;2
!n" PH:
pi@ adjustedto
with
osmodo
nt Added
"",no
'ranceof lostsoluIon:
mg N&CI o
mL sample
4
FC-206CF
mICROTOX(r) DATA SHEET Lot 101 5-min exo. J1884-4 7/19/91 sab
PAIR
1 2 3 4 5 6 7 E3
BLANK BLANK
CONC.
125.0()0 125.000 250.OC)O 250.000 500.000 5()0.()0() %1000.0()0 %looo.ol)()
Bo/Dt= too RATIO= t)-6700
lo/It
1 it).0/ 68.0 117.o/ 7().0 112.0/ 64.0
98.01 54.0 110.0/ 52.0 104.0/ 48.0
104.0/ 38.0 110.0/ 39.0
67
G-OBS
t).084 0.120 0.173 0.216 0.417 0.452
0.834 0.89c)
EC 50 = %1120.491,
923.602 TO %1359.357
EC50 '710 00 k-@ns11-
R =0.99039
SLOPE = 0.9361
INTERCEPT = +7.0215
G-EST
0.098 0.()98 0.203 0.203 0.419 0.419
0.867 1.)8.67
Lc>,.s
7.'7
11
ej 31 J45,
47
46
x
1-7 3e)
0 ----------------v----------------v----------------
RES.
PROB.
1 0.195
100
2 0.158
66
3 0.127
43
4 0.114
34
5 0.117
36
6 0.121
39 -1
7 0.174
80
8 0.181
86
--a-.
7-.
------- --------
-------
L
-6 ---
0
5-
<
A
-4 ---
m
t4
2->
--------
<A
2 ------- --
-----------------------
5 LOG CONC.
.-6
. .1 @@"-'OiCROT 0 X,
is
a Registered
Trade
Mark
of
Microbic%'Corporation.
7,
@14
FC-206CF Lot 101
MICROTOX(R) DATA SHEET 13-min exo. J1884-4 7/lq/91 sab
PA3 el
PAIR
CONC.
1
125.000
2
125.000
3
250.000
4
250.004)
5
500.000
6
500.000
7
%1000.000
8
%1000.000
BLANK Bo/Bt= 100 BLANK RATIO= 0.5300
lo/It
110.0/ 52.() 117.0/ 56.() 112.0/ 51.0 98.0/ 42.0 110.0/ 39.0 104.01 35.0
104.0/ 26.0 110.0/ 27.f)
53
B-OBS
0.121 0.107 0.164 0.237 0.495 0.575
1.120 1.159
G-EST
0.104 0.14)4 0.230 0.23() 0.51)7 0.5o7
1.118 1.118
(1)
q-7 14 li
J3 37 5;-3
51
17 35-
Sq
EC 50
= 885.997
733.348 TO %IOKO.421
.EC50 r- 2 q ( 7 3 0 - /Z>o o) rne, 14
R =0.9B770
SLOPE
0.8558
INTERCEPT = +6.7867
RES. 1 0.185 .2 0.19a -3 0.157 4 0.131 5 0.131 6 0.140 7 0.201 8 0.205
PROB. 82 93 58 41 41 47 96
100
0 ----------------V ---------------V -------------8-+
1 ol 1 1
-1 >
------- i
-------- I
-------
-6 -----
---5 --------
-----
L Io <6
1
-----
IG
A--
-2 >
IA
----------
-------------------------------------------------------
4
5 LOS CONC.
6
7@.
',MICROTOX is"@'&. Registered Trade Mark of MLcrob'ic@ Corporation.
,FC-206CF
Lot 101
MICROTOX(R) DATA SHEET 30-min exa. J1884-4 7/19/91 sab
PAIR 0
CONC.
1
125 000
2
125 000
3
250.000
4
250.000
5
500.000
6
500.000
7
%1000.000
8
%1000.000
BLANK Bo/Bt= loo BLANK RATIO- 0.5000
Io/It
110.0/ 48.0 117.9)/ 52. 1:) 112.0/ 45.0 98.0/ 37.0 110.0/ 33.0 104.0/ 30.0
104.0/ 20.0 110.0/ 21.0
50
G-OBS
0.146 0.123 0.244 0.324 0.667 0.733
1.600 1.619
G-EST
0.1300.1-30 0.300 0.300 0.690 1).690
1.590 1.590
03 11
'go
aq
ito Af@ll b;L iA
EC 50 = 676.026
612.149
00570 6 gc)
R =0.99517 SLOPE 0.8227
TO 746.568
7@5-o )
INTERCEPT
rnl6,/L
= +6.5162
f 6-c,6&)
RES. 1 0.119 2 0.129 3 0.091 4 0.081 5 0.082 6 0.085 7 0.127
8 0.128
PROB. 85
100 50 39 40 4S97 98
I ---------------v---0>
----------------
--B-t 1L 10 <
7
M ICROTOX
-2 --------------
j
4
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6
7
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