Document 5bwvp3gyjZm4M8DXdkd5Lzjx5

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid;may alsobe referredtoas PFOA, FC26, or FX-1001. (Octanoicacid,pentadecafluoro-C,AS # 33567-1) Remarks: 3M productiolnotnumber 269. The testsample isFC-26. It'psuritywas not sufficientclhyaracterizedt,hough currentinformation indicatesitisa mixtureof 96.5 - 100% testsubstance and 0 -3.5% C6, C7, and Cg perfluorohomologue compounds. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1974 Species: Pimephalespromelas Supplier:Not noted. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Graphed LC50 Test fishage: Not noted. Length and weight: Average length 2 inches Average weight 1.5 g Pretreatment: Not noted. Test conditions: Dilutionwater: Not noted. Dilutionwater chemistry- Not noted. Stock and testsolution preparation: Not noted Concentrations dosing rate:Once Stabilityof the test chemical solutions:Not noted Exposure vessels: Not noted. Number of replicates:one Number offish per replicate:Not noted. Number of concentrations: fiveplus a blank control Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 4.7 - 5.7 mg/L (controlexposure) 3.8- 5.2mg/L (375mg/L exposure)* pH range (24- 96 hours): 7.0- 7.2 (controelxposure) 6.0- 6.7 (375 mg/L exposure)* 375 mg/L testgroup (secondhighestconcentrationd)ata given because totalmortalitoyccurredinthe 500 mg/L testgroup (highest concentratiotnested)by 48 hours. Test temperature (24- 96 hours): 69 to 70'F RESULTS Nominal concentrations: Bk control,50, 125,250, 375, and 500 mg/L Element value: 96-hour LC50 = 440 mg/L (C.I.notcalculated) Element valuebased on nominal concentrations CONCLUSIONS The testsample 96-hour LC5o forfatheadminnow was determinedto be 440 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking3. Thisstudy lacksdocumentation and informationon the methodology. Sample puritywas notproperly characterized.Test concentrationwsere notcharacterized.Testing should have been done inreplicateH.ealthofthefishisnot known. REFERENCES This study was conducted by the3M Company, Environmental Laboratory,St.Paul,MN, Lab Request Number 2485, completed on November 10,1974. OTHER Last changed: 5/24/00 . 740101 ENVIROMMMAL ENGINEERING LADORATORY AQUATIC TOXICM WORK SHUT L-N .2 F-S Typ* Test Oi6@onyLl Test Organtas 7-a@-v\ Date Started Tint Started Dilution Water Ivin J62 -@-tel Material TostedF-C-a!@ Avg. weight Date Completed N.r CT . XOA -@-6Ct Avi Siz,--O I]-te-7-,1- - Platted LCSO mot Conc. 24 hrs % D.0 Survival 9H ppa 48 hro % D.0 Survival 1PH vM 72 hrs % D.0 S=Vival PH ppe 96 hrs % D.0 Survival PH Rpm Control -71@-5 -)7 70 5.@L 100 -- 00 -7214-7 '70, L7 5.C. -7o 6.5 ZA 1 -7,0 @A.-A loo 70 C-,q 4:5 100 -6.6 2)75 104 Averge LoMrature Coments: 1601 q.5 0 46qI 100 6,lq,A-.a 10O0ltt6 --77, to00 @4 tcso Calculation TT 'TU 0 10 20 30 40 $0 60 70 so 90 100 t Survival