Document 5bopEjkeqgxqyGgOLBj0wjDr4

BACK TO MAIN Study Title Growth and Reproduction Toxkity Test vith N2803-4 and the Freshwater Alga, Se;!enastrum capirfcornutum Data Requiremnt: Oiiidelino Ilumbaw U.E. EPA-TSCA, Guideline 797.1050 Authors Timothy J. Ward Peter L. Kowalski Robert L. 13oeri Study comp.1et.d narch 7, ,1996 Sponmoi: 314 Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, HinnesDta 55144 Te8ting FacLlity T.R. nilbury Laborrtories, Inc. 40 DOAkS 1,ane Harblehead, Hassachusettn 01945 T.R. nilbury study humber 896-TH Page 1 of 23 BACK TO MAIN T h i s mtudy vam conductod a c c o r d i n g t o &PA Goocl LAb0rAtOL-y P r a c t i c e Rogulationm ( 4 0 CrR 7 9 2 ) , With t h o f o l l o w i n g o x c e p t i c ~ n s . The s t a b i l i t y of t h o t o i t BUbstAnCe under t0.t c o n d i t i o n . v a s assumed but n o t v e r i f i e d a t T.R. H l l b u r y L a b o r a t o r f o r . D e v i a t i o n 8 from t h e s t u d y p r o t o c o l a r e prOBeflted i n S e c t i o n X I 1 o f thim roport:. Page 2 of 23 BACK TO MAIN 11. QUALITY MSURAMCI ETA- Submitted byr T.R. Wilbury Laboratories, Inc. 40 Doaka Lane mrblehead, Massachusetts Cmrtiticationr T.R. nilbury ~aboratories, Inc. study number 8 9 6 - ~ ~ , Growth and Reproduction Toxicity Test with N2803-4 and the Freshvater Alga, Selenastrum ~Caprfcornutum, VAS audited by the Quality ASSUrAnCe Unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory Practices1 (U.S. EPA, 1993). Quality alreurance audits vere performed anu the findings reported to T.R. Wilbury Laboratories management and the study director on the l'olloving dates: Aspect Audited Protocol: In-life : Draft Report: Final -port: Audit Date 8/ 4/95 9/26/95 1/18/96 3/ 7/96 Reported to Study Director 8/ 4/95 9/26/95 1/18/96 3/ 7/96 Reported to Management a/ 4/95 3/ 1/96 3/ 1/96 31 7/96 LA 3/:/?6 Quality Aeeurance o:Eficer T.R. Wilbury study Number 8 9 6 - ~ H Page 3 of 23 BACK TO MAIN 111. TmLI OI co#TtYTe SECZION: I. Cood Laboratory Practice Compliance statement 11. Quality Assurance statemevt 111. Tab10 of Contents IV. Index of Tables and Figures V. Summary VI. General Information VII. Introduction VIII. Hethodm and Haterials IX. Results X. References n. Signature Page XII. Protocol Amendments and DeViAtiOnSl Appendix A. Water Quality Data from Tho Toxicity Test PAGE 2 3 4 5 6 7 8 8 13 18 19 20 21 T.R. wilbury Study Number 896-TR Page 4 of 23 .- - . . _ I - - - BACK TO MAIN Table 1. Chemical characterization of a representative sample of water uaed to formulate test media for the toxicity test with the freshwater alga, Selenastrum capricornutum, and N2803-4.. Table 2. Test syatem sununary for t!m toxicity tost with N2003-4 And the freshwater alga, Selenitstrum Capri cornutum. Table 3. cell growth data from the toxicity test. with N2803-4 and the freshwater alga, Selenslstrum Capricornutum. Table 4. Average specific growth riite and percent change from the control from the toxicity test with the freshwater alga, selenastium capricornutum, and N2803-4. Table 5. Effective concentrations (EClOS, ECSOs, EC90s) from the toxicity test with the freshwater alga, selenastrum capricornutum, and ~2803-4. Table A . l . Temperature measured during the toxicity test with N2803-4 and the freshwater alga, Selenastrum Capri cornutum. Table A.2. pH valuer measured during the toxicity teat with N2803-4 And the freshwater alga, Selena:,trum Capri cornutum. Figure 1. Growth o f selenastrum caprfcornutum exposed to 82803-4 for 96 hours. PACE 9 11 14 16 17 22 23 15 T.R. Wilbury study Number 896-TH Page 5 of 23 I BACK TO MAIN v. I'u)o(Mr The t o x i c i t y of N2803-4 t o t h e freahwa,ter a l g a , selsnastruar caprlcornutum, i a d e s c r i b e d i n t h i s f i n a l report. The t e a t warn c o n d u c t e d f o r 3U Company f o r 96 hours from September 22 t o 26, 1995, a t T.R. Wilbury L a b o r a t o r i e s , Inc. i n Harblehead, Haaaachusetta. It waa conducted a c c o r d i n g t o t h e p r o t o c o l developed :for T.R. Wi1bu.q s t u d y Number 896-TH. N2803-4 wau s u p p l i e d by t h e sponsor. The t e s t waa performed under a t a t i c c o n d i t i o n s w i t h f i v e c o n c e n t r a t i o n s o f test substance rind a d i l u t i o n w a t e r c o n t r o l a t a t e m p e r a t u r e of 24 t 2.C. Nominal c0ncentrat;ions of N2803-4 w e r e 0 ( c o n t r o l ) , 62, 130, 250, 500, and 1,000 mg/L. The d i l u t i o n water warn sterile e n r i c h e d media a d j u s t e d t o a pH of 7.5. -Exporure o f a l g a e t o t h e test s u b s t a n c e f o r 96 hours r e s u l t e d i n a median e f f e c t i v e concentration (EC50) of 310 mg/L 32803-4 (95%c o n f i d e n c e i n t e r v a l 280 t o 3 5 0 mg/L) when c a l c u l a t e d u s i n g t h e number o f celle/ml, a n d g r e a t e r t h a n 1,000 mg/L when c a l c u l a t e d u s i n g t h e a v e r a g e s p e c i f i c growth r a t e . The 96 hour no obseinred e f f e c t c o n c e n t r a t i o n (NOEC) is 62 mg/L and t h e lowest observed e f f i t c t c o n c e n t r a t i o n (LOEC) is 130 mg/L when c a l c u l a t e d u s i n g t h e number of cells/ml and t h e NOEC is 500 mg/L and t h e LOEC i s 1,000 mg/L when c a l c u l a t e d u s i n g t h e average a p e c i f i c growth rate. Page 6 of 23 BACK TO MAIN V I . QIIZML IN?0RM&TION Haterial Temted: Sponaor I Teat Subatance Shipped Proms Reported Purity8 Study Initiation Dater Experimental Start Dater Experimental Completion Dater Study Completion Date: Archive Location or the Raw Data and a Copy of the Final Report I N2803-4 3n Company Environmental Laboratory 935 Bumh Avenue Building 2-3E-09 St. Paul, Minnemota 551144 3n Company Environmental Laboratory 935 Buah Awenue Building 2-3E-09 St. Paul, Minnesota 551.44 100% August 16, 1395 (protoccd signed) September 2!2, 1995 September 216, 1995 March 7, 1996 T.R. Wilbury Laboratories, Inc. Xarblehead, Mssachusetts T.R. nilbury study Number 896-TH Page 7 of 23 BACK TO MAIN . . V I 1 I*TRODIYCTIOM Thir mtudy warn rponrored by 3H Company. Tho objectivo of the rtudy warn to determino the 24, 48, 72, and 96 hour offoct:ivo concontrationr (EC101, ZCSOr, EC90r) and no obmerved offoct concentr&ion (HoCC) of tho test mubstance to the frerhwater alga, I:olona~trua,caprfcornutum, under rtatic conditions. The teet prOtOC01 warn bared on U . S . EPAmothodn (EPA, 1993). The report contains mections that doscribs the methods and materials employed in the mtudy, And reaults of the invertigation. The report also contains an appendix that: presents water quality data collected during the test. VIII. HETBODS AND MATERIALS TIST SUBfbTAWCkr The sample of N2803-4 (T.R. wilbury Laboratories Sample Number 522) was delivered to T.R. Wilbury Laboratories on August 14, 1995 in a cardboard box at ambient temperature. It was contained in u1 50 ml glass jar. The label attached to the bottle included the fo1:lowing information: "N2803-4, (see U S D S ) " . N2803-4 (white powder) was supp1.ied by 3M Company, St Paul, Hinnesota. Prior to use the test substance was mtored in the dark at room temperature. The test substance was asnumed to havei a purity of 1001 active ingredient and to be stable under storage and testing conditions. Unumed teat substance will be returned to the sponmor. , .,.. DILVTIOH HATER: Water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978; T.R. Wilbury standard Operating Procedure number 6 ) adjusted to a target pH of 7.5 with 0.1 N HCl prior to use. characterization of a representative sample of water used to formulate media is presented in Tabls 1. TEST ORGAUISU: Algae used for the test (Selenastrum caprfcornutum, VTEX 1648) vas from a c u l t u r e originally procured f r o m t h e culture COllleCtiOn of Algae at the University of Texas at Austin and delivered to T.R. wilbury Laboratorios on April 11, 1995. The cu1t:ure war transferred to aterile enriched M d i A identical to media uaed for this teat and maintained at test conditionm for at least 14 days beforo the definitive teat. The subsmple of algae used to incculate media.at tho start of the definitive test c a w from an 8 day old culthre. Identification of the culture 0rgAnim.u War verified uming an appropriato taxonodc :key. ". T.R. Wilbury Study Number 896-TH PAge 8 Of 23 . -.. ..,. -. BACK TO MAIN T a b l o 1. chomical c h a r a c t o r i r a t i o n of a r o p r o a o n t a t i v e a m p l o of wator uaod t o f o r m u l a t e t o s t modia f o r t h o t o x i c i t y tomt w i t h t h o f r o a h w a t o r alga, s o l o n # ~ t z u a rcspricornutum, and N2803-4. Parmoter' u n i t of Heasurement Detection Limit Uaaaured Valu. notala Aluminum Araenic Boron cadmium Chromium Cobalt Copper Iron Lead Mercury Nickel silver Zinc Nitrate Chloride Fluoride Total Organic Carbon Total Phosphorue Organochlorine Pesticides Toxaphene Organophosphorus Pesticides Dhthoate TEPP Uonocrotophos 0.1 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02 0.05 1 0.1 1 0.03 0.5 2 0.5 2.0 2.0 2.0 0.5 ND' ND ND ND ND ND ND 0.03 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND Notear 3" 1. P a r m e t e r a weremeaauredindeionizedwater ( u s e d t o f o r m u l a t e d i l u t i o n vater) t h a t was c o l l e c t e d d u r i n g August, 1995 and a n a l y z e d by Pace, Inc. as p a r t of r o u t i n e water q u a l i t y testing. i 2. ND I not detected a t or above t h e detection l i m i t . T.R. Wilbury study Number 896-TH Page 9 of 23 BACK TO MAIN TOXXCITX TtlTi Roaulta of A range finding teat were uried to meloct tho conconttations for tho definitivo test. The range finding temt was - - priormod from September 7 to 11, 1995. At the concluiaion number of c ~ l h / m lequalled the following percent of the 1.0, 10, and 100 mg/L ~ 1 0 0 1and 1,000 mg/L 8%. of tho toat tho control: 0.10, The definitive toxicity test was performed from September 22 to 26, 1995, according to T.R. nilbury Protocol 896-TH (Growth and Reproduction Toxicity Teat with N2803-4 and the! Freshwater Alga, Selonastrum caprfcornutum). The protocol, which waii signed by the atudy director on August 16, 1995, i s based on procedures of the U.S. Environmental Protection Agency (1993). A summary of the test system ia pre8ented in Table 2. The test was conducted at a target temperature osf 24 f 2-12 with five concentrations of teat substance and a dilution water concrol. Nominal concentrations of the test substance were: 0 mg/L (control), 62, 130, 250, 500, and 1,000 mg/L. A 1,000 mg/L stock solution was formulated on September 22, 1995 by adding 0.5 g !r280:1-4 to sterilfl enriched media and adjusting the final media volume to 500 ml. Appropriate amounts of this atock solution were added directly to dilution water to formulate teat media. Algae were distributed among threa replicates cbf each treatment at the rate of approximately 10,000 cellslml. The tefrt was performed in 250 ml glass Erlenmoyer flasks that contained 50 ml. of test Solution. ' Test vessels were randomly arranged on a rotary shaker adjusted to 100 rpm in an incubator during the test (a random numbers table was used t o select the location of each vessel). A 24 hour light and 0 hour dark photoperiod waa automatically maintained with coo:l-white fluorescent lights that provided a light intensity of approximately 400 footcandles. The number of algal cells/ml in each test vessel. and the occurrence of relative size differences, unusual cell shapes, cdars, 'locculations, adherence of cells to test containers. or aggregation of cells was determined visually by means of direct microscopic examination with a hemocytometer. cell counts were made and recorded daily during the 96 hour test. The determination of whether toxic effects were algistatic or algicidal was performed at tho conclunion of the t o x i c i t y test (0.5 m l of media from each 1,000 mg/L vessel. was transferred to a venae1 containing fresh media without N2803-4 and incubated for 72 hours under t e a t conditions). T.R. Wilbury Study lumber 896-TH Page 10 of 23 BACK TO MAIN Table 2. T e a t myitem runmury f o r t h a t o x i c i t y teml: w i t h N2003-4 and t h e freshwater alga, selenaatrua caprfcornutum. specie. : Culture Acclimation Period: Temt Duration: T e a t Vessels: Volume of Test nedia: N u m b e r of R e p l i c a t e T e s t Vesrrels: Inoculumr O a c i l l a t i o n Rate: Incubator Temperature Range: Photoperiod: Light Intensity: Water Q u a l i t y ifeasuremente: End Points: Acceptability Criteria: selenaatrum cap.rfcornutum >14 days 96 hours 250 m l g l a s s flrisks 50 m l 3 -10,000 cells por m l 100 rpm 23.5-23.7.C 24 hours l i g h t and 0 h o u r s d a r k . -4110 footcandles1 pH i n each t e s t vessel a t t h e beqinning and end of t h e test. Incubator temperature daily. Di:!ution water a n a l y s i s i a suermarired i n Table 1. 2 4 , 48, 72, and 96 h o u r ECIO, EC50, ant1 EC90; no obsierved e f f e c t concentration. Loqarithmic growth a t 96 hours and acceptable temperature range. T.R. Wilbury s t u d y Number 896-~H Page 11 of 23 BACK TO MAIN . . -.... TmmpmrAtUrm of thm i n c u b a t o r wns m a s u r a d and rmcordmd d a i l y (themommtmr numbmr 2968), and pH ( e a c h n m d m l p a I 12 motmr; i n r t r u a o n t number 124) warn dmtmrminmd in each telmt vmrrel a t t h e b e g i n n i n g and mnd o f t h e t e a t . The tmnporature in a rmprosmntativm vmmsml of w a t m r i n c u b a t d w i t h thm tmat vmrrmlr war c o n t i n u o u s l y recorded. .. STATISTICAL HBTEODSI The avmrage a p e c i f i c growth rate was c a l c u l a t e d as thm n a t u r a l l o g of t h e number of cells/ml A t 24, 48, 72, and 96 hours minus t h e n a t u r a l l o g o f t h e number of cells/ml a t 0 hours d i v i d e d by t h e e x p o s u r e p e r i o d . The p e r c e n t change from t h e c o n t r o l was c a l c u l a t e d by s u b t r a c t i n g t h e treatment average s p e c i f i c growth rate from t h e c o n t r o l average s p e c i f i c growth rate, dividing t h e difference by t h e average s p e c i f i c growth rate i n t h e c o n t r o l , and m u l t i p l y i n g t h a t v a l u e by 100. R e s u l t s of t h e t o x i c i t y t e . i t were i n t e r p r e t e d by s t a n d a r d s t a t i s t i c a l techniques. The binomial/interpolat.ion or p r o b i t methods ( s t e p h a n , 1983) were used f o r c a l c u l a t i n g EC10, E:C50, and EC90 values. A l l c a l c u l a t i o n . were performed u s i n g t h e number of cells/ml and t h e averagm s p e c i f i c growth r a t e s and nom.ina1 c o n c e n t r a t i o n s o f N2803-4. The no observed e f f e c t C o n c e n t r a t i o n (NOEi:) was d e t e r a i n e d rrsing a p a r a m e t r i c one-way a n a l y s i s o f v a r i a n c e (ANOVA) or a non-parametric Kruskal and Wallis' test and t h e number of c e l l s h l and t h e average s p e c i f i c growth rate in each test vessel a t t h e end of the test. T.R. W i l b u v Study Number 896-TH Page 12 of 23 BACK TO MAIN . .. no insoluble material waa notod in any test vessel during tho test. The algal population in the control vosae:!s grew wall, resulting i n an average of 1,407,000 cells/ml after 98 hours (Tablo 3). Water quality throughout the test waa within acceptable limits (Append.Lx A). Tho range of incubator temperatures was 23.5 to 23.7.C (T.ble A . 1 ) . The pE of test media (Tablo A.2) was slightly affected by the teat substance only at 1,000 mg/L at the beginning of the tost. Biological data generated by the acute toxicity t'est with 12803-4 are presented in Tables 3 and 4. The growth curve for algae exposed to the test substance for 96 hours is prosanted in Figure 1 (;thealope of the dose-response curve is 2.5 based on cells/mL at 96 hours). The 7 2 and 96 hour EC50 value!, for algae exposed to N280.1-4 are presented in Table 5. The 96 hour EC50 (and associated 95 percent confidence 1fDiits) 1s 310 mg/L ( 2 8 0 and 350 mg/L) when calculated using the number of cells per ml, and greater than 1,000 mg/L when calculated using the average specific growth rate. No effects (size differences, uiiusual cell shapes, colors, flocculations, adherence of cells to test container!,, 01: aggregation of cells) were noted. The 96 hour no observed affect concentration (NOEC) is 62 mg/L and the lowest observed effect conc:entration (LOEC) is 130 mg/L 32803-4, when calculated using the number cells/ml, and the NOEC is 500 mg/L and the LOEC is 1,000 mg/L. when calculated uaing the average specific growth rate. The determination of whether toxic effects were1 algistatic or algicidal was performed at the conclusion of the toxicity test. A 0.5 m l aliquot of media from each 1,000 mg/L vesso:L was transferred to a vessel containing fresh media without N2803-4 and incubated for 72 hours under test conditions. Algae increased from <10,000 cella/ml to 642,000 cells/mG, indicating that the effect of N2803-4 at this concentration was algistatic rather than algicidal. T.R. Wilbury study Number 896-TH Page 13 of 23 BACK TO MAIN Tablo 3. C e l l growth data from tho acutim toxicity tort with N2803-4 and the frorhwater alga, 8elenut.ntm capricornutun. Nominal Concontration of N2803-4 (Qgm Rep. Numbmr of C e l l s / m l . x 10' (hour) 0 24 48 72 96 0 (control) 62 130 250 500 1,000 1 2 3 mean % control 1 2 3 mean % control 1 2 3 mean % control 1 2 3 mean % control 1 2 3 mean a control 1 2 3 mean % control 10 16 10 18 10 12 -1-0 -1-5 lo 17 10 15 10 15 10 16 100 107 10 16 10 19 10 10 10 15 100 100 10 14 10 15 10 11 10 13 100 87 10 11 10 510 10 13 10 11 100 73 10 .<LO 10 ,<lo 10 10 10 at10 100 *e67 142 466 1,544 154 584 1,320 180 592 1,356 1-5-9 54-7- 1,40-7- 162 602 1.446 184 576 1,272 168 586 1,616 17 1 588 1,445 108 107 103 160 556 1,080 144 598 1,184 136 584 1,124 147 579 1,129 92 106 80 138 590 762 174 552 752 140 528 744 151 557 753 95 102 54 92 274 354 104 2 54 484 110 300 492 102 286 443 64 52 31 90 106 200 62 134 216 72 124 128 75 121 181 47 22 13 T.R. w i l b u r y Study Number 896-TH Page 14 of 2 3 BACK TO MAIN 1 ". 0 0 r-*Conlrds -e-250 mg/l 24 48 72 96 Hws * +6 2 mg/l 500 mg/l ----1 -)I130 mg/l -A-1000 mg/t Figure 1. Growth of Selenastrum capricornutum exposed to N2803-4 for 96 hours. T.R. Wilbury Study Number 896-TH Page 15 of 23 BACK TO MAIN f 5 A. Tablo 4. Avorago rpocific growth rat0 and p r c o n t chaago from tho control from tho toxicity tamt with tha fromhwator alga, ) . 8denastrua caprlcornutuat, and N2803-4. Nominal Concentration of N2803-4 w/L) AVOS6ge S p O C i f f C Growth Rata - 24hr 48hr 72hr 96hr 0 (control) 0.017 0.058 0.056 0.052 62 0.020 0.059 0.057 0.052 130 0.017 0.056 0.056 0.049 250 0.011 0.057 0.056 0.045 500 0.004 0.048 0.047 0.039 1,000 0.000 0.042 0.035 0.030 Portent Change From Control 24hr 48hr 72hr 96hr -- -- -- _- -18 -2 -2 0 0 3 0 6 35 2 0 13 76 17 16 25 100 28 37 42 .. . , T.R. Wilbury Study Number 896-TH Page 16 of 23 BACK TO MAIN Tablo 5. E f f o c t f v e c o n c o n t r r t i o n r (EClOs, ECSOs, LC9Or) from t h o toxicity tost w i t h t h o f r e s h v a t o r alga, S d O n ~ 8 cta~p r i c o r n u t m , and N2803-4. Th EC Value (W1.b 95 Percent Confidence Limits (WL) C a l c u l a t e d Wing t h e Wumbor of Coll~i/mL 72 hours 96 hours EClO ECSO EC90 - = = 310 520 >1,000 EClO = 97 EC50 = 310 EC90 .i 1.000 - 110 440 - 250 1,000 - 800 >1,000 -77 120 - 280 350 - 830 >1,000 C a l c u l a t e d Uring t h e Average Specific Growth Rat. 72 hours 96 hours EClO = 470 EC50 >1,000 EC90 = >1,000 EClO = 220 EC50 = >1,000 EC90 = *1,000 380 - 550 >1, 000 >1,000 160 950 - - 280 >1,000 >l,OOO T . R . Wilbury s t u d y Number 896-TH BACK TO MAIN Bruce, R.D., and VerDteecJ. 1992. Continuous Toxicity Data. No.10, pp. 1,485-1,494. A StatiaticaL Procedure for Modeling Environ. Toxicol. and Chum. V01.11. stephan, C.E. 1983. Computer Progi:m for Calcul.ation of LC50 Values. Peraonal communication. U . 9 . EPA. 1993. 40 CPR Part 797.. Toxic substances Control Act Test Guidelines; Final Rules. Section 797.1050. U . 9 . EPA. 1993. 40 CFR Part 792. Toxic Substances Control Act (TSCA); Good Laboratory Practice Standarde; Final Rule. T.R. wilbury Study Number 896-TH. Page 18 of 23 BACK TO MAIN X I . I I O N A T U U PAQE &dy Director Aquatic Toxicologiat Aquatic TOxic'i;lo%ist Biologist Cowthor 4t.hor T.R. Hilbury S t u d y Number 896-Tu Page 19 of 23 BACK TO MAIN 111. PROTOCOL hMENDMUTS AND DWIATIOUS The protocol was amended to specify the nominal concentrations for the definitive study. No other amendments were made to the protocol. PROZDCOL DEVIATIONS: Two nominal concentrations were slightly diliferent than the ones listed in the protocol. This deviation did not affect the outcome of this test and no other deviations were mado from the protocol. T.R. wilbury study Number 896-TH Page 20 of 23 BACK TO MAIN Appondix A: UATER QUALITY DATA PROW TBt MXICITI TEST T.R. Uilbury study Number 896-TH Page 2 1 of 23 BACK TO MAIN Tablo A . l . Temperature measured during the toxiclty test with ta2803-4 and the freahwater alga, Se.t.na8trm c a p r i c o r n u t w . Hour of Exposure Temperature of Incubator f * c ) 0 23.5 24 23.6 48 23..6 72 23.,7 96 23 ., 5 T.R. wilbury study Number 896-TH Page 22 of 2 3 BACK TO MAIN Tablo A.2. pH value. Mamured during tho t o x i c i t : y t o a t w i t h N2803-4 and t h e frmmhwator a l g a , so;!onamtrun c a p r l c o r n u t w . Nominal concontration of N2003-4 (WL) ~ ~ ~ Rctplicato PB Initial Final 0 (control) 1 2 3 65 1 2 3 130 1 2 3 250 500 1,000 1 2 3 7.5 8.2 7.5 9.6 7.5 9.6 7.5 8.9 7.5 9.0 7.5 8.6 7.5 6.4 7.5 6.5 7.5 6.5 7.5 5.8 7.5 5.4 7.5 5.4 7.5 5.1 7.5 5.0 7.5 5.0 7.4 5.4 7.3 5.4 7.3 5.5 T.R. WilburL s t u d y Number 896-TH Page 23 of 2 3 - LIV v A n u I v n L r v I b . r - ARCHIVED FINAL REPORT IBACK TO MAIN LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01 /1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX WORK CONTRACT LAB(S): TRWIL EXP COMP DATE: 10/04/ 1995 DATiE COMPLETED: 03/27/1996 PROJECT LEAD I PHONE NO: 3M IFAX NO: 6!51-778-6176 Samples received from S.J . M a r t i n . Samples 1 - 5 sent t o TRWIL on 8/11/95. 8/22/ 95. An a d d i t i o n a l 4L o f sample 5 sent on Sample 1 = T e t r a b u t y l ammonium hydroxide, 40% i n water. Sample 2 = L-13492 (45% s o l i d s , 27% water, 27% IPA) Sample 3 = FC-26, 100% s o l i d s . D i s s o l v e i n 50:50 water:IPA s o l u t i o n . Sample 4 = FC-143, 100% s o l i d s . Sample 5 = L-13856, 15% s o l i d s . Sample 6 = L-13856, d i l u t e d by a f a c t o r o f 15, t e s t e d as an e f f l u e n t . Sample #1 (Tetrabutylammonium hydroxide) - a new b o t t l e was ordered from A l d r i c h because TR Wilbury needed more sample. T h i s idas l o t 05922BM, r e d l a b e l , c a t . # 17,878,O. sab 9/11/95 NOTE: A l l data f o r sample 3 (FC-26) i s r e p o r t e d i n terms o f FC-26, and n o t as a 50/50 m i x t u r e w i t h isopropanol. sab 11/21/95 NOTE: For sample #5, o n l y a r a n g e - f i n d i n g t e s t was run f o r t o x i - c i t y t o algae. The requestor canceled t h e request f o r t e s t i n g so no d e f i n i t i v e ! t e s t was run. The r e s u l t s f o r t h e range f i n d e r a r e as f o l l o w s : Test Conc. mg/l 0.1 1.0 10 100 1000 EC50 (95% C.I.) sab 2/16/96 Percent Difference i n Avg. S p e c i f i c Growth Rate! 96-hrs Expsure 2 18 4 32 - 100 160 (100 1000) mg/L. For N2803-6, t h e t e s t i n g was canceled before a d e f i n i t i v e study with f i s h c o u l d be completed. Results r e f l e c t t e s t i n g w i t h a sjtngle concentration o f 13% v/v, which showed no m o r t a l i t y a f t e r 96-hours. sab 3/14/96 1 08/11/95 TB NH40H L o t 10317KQ TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST 27 MG/L (23 - 32) 04/01 /2001 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT BACK TO MAIN 2 1 0 8 / 1 1 / 9 5 TB NH40H L o t 10317KQ - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0 2 L- 13492 Lot 2 720 */I, 3.7 MG/L - ( 6 0 0 - (1.8 1000) 7.7) TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL IC50-ALGAE *ALGAE ASSAY I C 5 0 62 MG/L 960 MG/L 8.4 MG/L (36- 100) - ( 8 3 0 - > 1 0 0 0 ) (5.9 14) 3 FC-26 L o t 135 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL IC50-ALGAE *ALGAE ASSAY I C 5 0 3 6 0 MG/L 140 MG/L 9 0 MG/L (300 - 500) (110 - 180) (63 - 130) 4 FC-143 L o t 427 - TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY I C 5 0 5 0 8 / 1 6 / 9 5 L-13856 As - is 7 2 0 MG/L 740 MG/L >IO00 MG/L - (600 1000) (660 - 830) (-1 - TRWIL 48-HR-EC5O *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0 6 L - 1 3856 1 :15 diluted TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL ICSO-ALGAE *ALGAE ASSAY I C 5 0 190 MG/L (13 0 - 6 0 0 ) >IO00 M;/L (-1 SEE COMMENT ABOVE 1.2 % V,W (1.0 >13 % V j V (-1 TESTING CANCELED - 1.7) * = CONTRACT LAB DATA