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COVANCE> Sponsors: APME Ad-Hoc APFO Toxicology Working Group PROTOCOL Study Tite: 26-Week Capsule Toxicity Study with Ammonium Perfluorooctanoate (APFO) in Cynomolgus Monkeys Date: September 23, 1998 Performing Laboratory: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Proposal No. w6806a Covance 6329-231 T- (%%9 02518 : -_-- Covance 6325231 Pag6e2.r Study 26-Week Capsule Toxicity Cynomolgus Monkeys Study with Ammonium Perfluorooctanoate (APFO) in Purpose To assess the effectofthe test material on critical enzyme levels, hormones, and other selected biochemical parameters `monkeys for at least 26 weeks when administered daily by capsule to. cynomolgus Sponsors 'APME Ad-Hoc APFO `Toxicology WorkingGroup Study Monitor Paul Lieder, PhD, DABT 3M `Toxicology Services `Building 220-2E-02, 3M Center St. Paul, Minnesota, 55144-1000 Telephone No.: 612.737.2678 Facsimile No.: 612.733.1773 Alternate Study Monitor John Butenhoff, PhD, DABT 3M "Telephone No.: 612.733.1962 Facsimile No.: 612.733.1733 Sponsor's Representative David Farrar, PhD ICI Chemicals & Polymers Limited Occupational Health `The Heath, Runcorn, PO Box 13 Cheshire, WA7 4QF Telephone No.: 01928.513953 Facsimile No.: 01928.515146 CSotvuadnycLeoLcaabtoiroantories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 coR514 -_-- Mailing Address: PO Box 7545-2595 Madison, Wisconsin 53707-7545 Study Director Peter J. Thomford, PhD `CToevlaenpchoenLeaNboo.r:ato6r0i8e.s24In1c..7207 Facsimile No.: 608.242.2736 [-- Pages Page3 `Toxicologist Dale Aldridge, BS Covance Laboratories Inc. EPxrpoeproismeedntSatluSdtyarTtiDmaettea:blSecpiember 29, 1998 In-Life Start Date: September 29, 1998 In-Life End Date: July 2, 1999 AExupdeirtiemdenDrtaafltTReerpmoinrattDiaotne:DaOtec:toTboerb2e2d,e1t9e9r9mined Regulatory Compliance "This study will be conducted in compliance with the Environmental ProtectioAn gency `Good Laboratory Practice Regulations as set forthin Title 40 of the US Code of Federal Rweigtuhlaatniyoanpsp,liPcaarbtl7e9a2,meinsdsumeednNtsoveexmcebpetrt2ha9t, b1i9le83ac(iedffdeectteirvmeiDneatcieomnbsedron2e9,by19U8n3i)v,earsnidty of Dundee will not be done in compliance with GLPs. `Animal Care and Use Statement All procedures in this protocolarein compliance with the Animal Welfare Act Rneogtuulnanteicoensss,ar9iClyFdRupl1-i4c.atIenatnhye opinion of the previous work. Sponsor and. study director, the study does Quality Assurance `ATshseuprraontcoecoUln,istt(uQdyAUc)on.duTchte, aprnodliffienraaltrieopnocretllwinlulcbleeaaruadnittiegdebnyevtahleuCaotivoanncdaetaQuaanlditryeport `will be audited by the QAU of Pathology Associates International. The serum, urine, feces, and liver APFO analysis and report will be audited by the QAUof 3M Environmental audited by the Labor QAU atories. The of Ani Lytics blood hormone Inc and DuPont dete , as rminations appropriate and report . The rece will ptor be level determinations determinations and report will be audited done by the University of DbuyntdheeeQwAilUlonfotDbuePoaundti.tedB.ile acid c02518 "Test Material Identification Ammonium Perfluorooctanoate (APFO) Lot Number "The lot number will be maintained in the rawdata. Cones capess Responsibility of the Sponsor Stability Responsibility of the Sponsor Storage rn. Conditions Characteristics tIensftomramtaetriioanl oisnosnynftihleeswiistmhetthheoSdpso,ns`ocro.mposition, or other characteristics that define the Gelatin Capsules Ca be pssuuplpelsie(dSibzye tNhoe.m2a)nuofbatcatiunreedr.froGemlaTtoirnpacca,psIuncl.es(Fwaiilrlfibeelds,tNoreedw aJterrsoeoy)m; tleomtpneuramtbuerre.wi ll A `copy of the Certificate of Analysis provided by the manufacturer will be maintained in the data. Reserve (Archive) Samples Areserve sampleofeach lot of test material (1 g each) will be taken and stored at room temperature. These samples will be transferred to the Sponsor after completion of the in-life phase. Disposition of Test Material `Any remaining test material will bereturned after authorization from the Sponsor. Animals Species Cynomolgus monkey 02510 _-- Source Covance Research Products Inc. Age at Initiation of Treatment Young adult/adult Covance 3h2Pu9a2eg3es1s Weight at InitiationofTreatment 3to5kg Number and Sex: 22 males Identification Collar tag Husbandry Housing Individual; animals will be housed in suspended, stainless steel cages. Diet not require analysis. Certified routinely primate diet analyzed by (#8726C, Harlan the manufacturer Teklad) once for nutritional or twice. daily. The diet is components and environment al contaminants. Results of specified nutrient and contaminant:analyses are on file at Covance-Madison. Fruit, vegetables, or other supplements may be provided but will `Water Ad libitum. Samplesofthe water are routinely analyzed for specified microorganisms and environmental contaminants. The results are on file atCovance-Madison. Contaminants `There are no known contaminants in the diet or waterat levelsthatmight interfere with this study. Environment Environmental controlsforthe animal room will be set to maintain 18 to 29C,a relative humidityof30 to 70%, and a 12-hour light/12-hour dark cycle. Acclimation Minimum of 4 weeks co251F Covance 6329231 -_-- Pagce6s Randomization bAlnoicmkaslesquwialllbtoetwheeignhuemdb,erstorfataifniiemdablys btoodbey wseeliegchtte,dafnodr eaallcohcgatreodupt.o tAhneinmuamlbseirnoefach block will then be assigned to groups using computer-gencrated random numbers Justification AnPoFnhOuimsanakpnriomwatneshe(psaulcihc apserthoexicsyonmoemoplrogluisfemraotnokre(yP)P)reinsptohnedrasti.miWlahrelynteoxhpuomsaendsto PP, s(pie.ec,ielso.w to no hepatic response) and therefore are an appropriate human surrogate. Group Designations and Dosage Levels Dose Level Group (mg/kg/day) Number of Males T (control) 2 (low-dose) o 3 4 3 (mid-dose) 10 6 4 a (high-dose) The control group (Group 1) 30 will receive empty gelatin 6 capsules. b Ttrweaoteadnfiomral2s6iwneGekrso,uptshe1n, t3,reaantdme4ntdewsiilgnbaeteddisacsorneticnouveedr,yaanndimtahlesawniilmlables twiolxlicbeeffoebcstesrvfoerdaftolreraesvter1s3ibwileietky,s ppeorsstitsrteeantcme,enotr. dBelaasyeedd oonccpulrarsemnaceteosft rmeatdeurcieadl loerveexltseannddedcliantitcahle pdaistchroeltoigoynoffitndhiengss,tutdhye drierceocvteorryamftaeyr be `consultation with the Sponsors, Dosing Procedures Method of Administration Orally by gelatin capsules, daily (7 days/week) for at least 26 weeks Reason for Dosing Route `To compare with previously conducted toxicology studies using the oral route coz518 _-- se Covmee 6e32w9e.e3rr1 Dose Preparation Capsules will be prepared at least weekly. The size and number of capsules willdepend on the physical monkey. Dose characteristics of the te levels will be based on st th mater e test imaalt,ertihaeldaossesulpepvleile,d.andIntdhieviwdeuiaglhdtaiolfythe doses will be based on the most recent individual body weights, with the exception of body weight preparations collection days when the previous `body willbestored at room temperature until weight dosed. will be used. All dose Dose Analysis Becausethetest material is not being mixed with a vehicle, dose analyses are not required. Observation ofAnimals Clinical Observations `Each animal findings will will be observe be recorded as d twice daily (a.m. they are observed. and p.m.) for mortality and moribundity; `Each animal will be observed daily and food consumptionwillbe assessedqualitatively; abnormal abnormal findings findings, will be recorded. oran indication Once weekly, each animal willbe observed; that theanimalis normal will be recorded. `Additional findings willberecorded as theyare observed. Body Weights Weekly before initiation of treatment, Day -1(for the Day 1 dose calculations),on the first day of treatment, and weekly thereafter Ophthalmic Examinations Before initiation oftreatmentand before each scheduled sacrifice. The anterior portion ofthe eye, optic media, ophthalmoscope. and ocular fundus will be examined using an indirect Clinical Pathology Frequency Unscheduled Collections s`aWchreinfipcoesdsaitbluen,scbhleooddulweidllinbteercvoalllsected for clinical pathology tests from animals cozspq Covance 6329231 Pages -_------m-------- Pages Scheduled Collections Before initiation of treatment; and (before the daily dose) afterat least 30, 60, 90, and 180 days of treatment; and after at least 30, 60, and 90 days of recovery Number of Animals All Method of Collection Animals will be fasted overnight; blood willbecollected from afemoral vein. Anticoagulants will be sodium citrate for coagulation tests and potassium EDTA for `hematology tests. No anticoagulant is required for clinical chemistry tests. Urine `will be collected on wet ice ovemight (approximately 16 hours). Tests Hematology red blood cell (erythrocyte) count platelet count hemoglobin `white blood cell (leukocyte) count `hematocrit `mean corpuscular volume differential blood cell count blood cell morphology `mean corpuscular hemoglobin reticulocyte count `mean corpuscular hemoglobin `concentration Coagulation activated partial thromboplastin time ~~ prothrombin time. fibrinogen Clinical glucose urea nitrogen creatinine total protein albumin globulin total bilirubin direct bilirubin (if total bilirubin is greater than 2.0 mg/dL) cholesterol triglycerides alanine aminotransferase alkaline phosphatase. Chemistry aspartate aminotransferase `gamma glutamyliransferase sorbitol dehydrogenase creatine kinase calcium inorganic phosphorus sodium potassium chloride bile acids amylase lipase `pancreatic-specific amylase cozs20) _----m Covance 632P9a2g5e1s Paged appearance. volume (approximately 16 hours) pH`specific gravity protein urobilinogen Urinalysis glucose ketones bbilloiordubin `microscopic examinationofsediment Blood Hormone Determination Frequency `Three times before initiationoftreatment; afattleearst 30, 60, 90, and 180 days of treatment; and afterat least 30, 60, and 90 days of recovery Number of Animals All Method of Collection Animals will notbefasted; blood will be collected from afemoral vein (an alternative vein may beused if necessary). Approximately 6mLof blood for plasmasamples `willbe collected into tubes with potassium EDTA as theanticoagulant. Aanptpircooxaigmualatnetl y a n6d mL of allow ebdlotoodc for lot. sBelrouomdssaammpplleesswiwlilllbbeecomlaliencttaeidnweidtahtourtoom temperature until serum plasma s harvested. is harvested. Blood samples will be maintained chilled until Serum Sample Handling woSamples for serum willbecentrifuged within 1 hour after collection, and serum will bsethoraerdveisntaedf.reeSzeerrusmetwtiollmabiendtiaviinde-d60inttoo-t8w0oCapupnrtoilxipmaactkeeldy equal aliquots on dry ice and and shipped Dr. Saroj Das Ani Lytics Inc. 200 Girard Street, Suite 200 Gaithersburg, Maryland 20877 "Telephone No.: 301.921.0168 Facsimile No.: 301.977.0433 Ani Lytics Inc. will be notified regarding shipment of samples. cozszf Covance 6329231 -_-- -- PaPgageel10l Serum Tests Sstaimmpulleatsinwiglhl obremaonnael,yztoetdalbaynAdnfireLeyttircisioIdnoc.t,hyfroorneisntera(dTi3o)l,, estrone, and total estriol, thyroid and free thyroxin (T4). Results will be provided for inclusion in the final report. Plasma Sample Handling `Samples for plasma will be centrifuged harvested and storedin a freezer set to within 1 maintain houraftercollection. Plasma -60 to -80C until packed on will dry be ice and shipped to: Jon C. Cook, PhD, DABT E. I du Pont de Nemours & Company, Inc. Haskell Laboratory for Toxicology Building 11 Room 1325 and Industrial Medicine 1090 Elkton Road Newark, Delaware 19711-0500 `Telephone No.: 302.366.5495 Facsimile No.: 302.366.5003 DuPont will be notified regarding shipment of`samples. Plasma Analysis Samples will be will be provided analyzed by DuPont Haskell `Company for inclusion in the final report, for cholecystokinin. Results Serum APFO Level Determination Frequency Afte7r days of treatment and every 2 weeksthereafter during treatment andrecovery Number of Animals All Method of Collection Animals will not be fasted; blood (approximately 2 mL) will be collected from a femoral vein (an aliemative vein may be usedif necessary) without an anticoagulant. Sample Handling Samples will be centrifuged within 1 hour after collection, and serum willbe harvested. Serum samples will be stored ina freezersetto maintain -10 10-30C. 00252 -_-- Sample Shipping Serum samples will be packed on dry ice and shipped to: Kris J. Hansen, PhD 3MET.&S Bldg. 2-3E-09 935 Bush Avenue St. Paul, Minnesota 55106 Cove 632931 Pmgeeint Kris J. Hansen, PhDorheralternate will be notified regarding the shipment ofthe samples. Analysisof serum for APFO will be done on the samples. Results will be provided for inclusiion the final report. possible future analysis. `Additional Blood Collection At scheduled and unscheduled sacrifices, blood (as much as possible, collected from animals at the time of exsanguination. Approximately up to 20 mL) equally sized will be samples of serum (collected without anticoagulant) and whole blood and plasma (both collected using EDTA as an anticoagulant) from each animal will be transferred into containers (approximately 5 mL each). Whole blood, serum, and plasma samples will be stored ina freezer set to maintain -60 to -80C until shipped to 3M (Kris Hansen) for Urine and Feces APFO Level Determination Frequency After 7 days oftreatmentand every 2 weeks thercafter during treatment and recovery (concurrent with serum APFO sample collection) Number of Animals All Method of Collection oAnviemmailgsh.willnot be fasted; urine (collected on wet ice) and feces willbecollected Sample Handling Samples of urine (at least 2 mL) and feces (at least 5 grams) will be stored in afreezer set to maintain -10 to 30C. 02528 -_---------- Covn-- ee 63P2a9g2e3r1 Page Sample Shipping `Samples will be packed on dry ice and shipped to: Kris J. Hansen, PhD B3lMdgE.T2.38&-S09 935 Bush Avenue St. Paul, Minnesota 55106 Kris J. sample sH.anAsneanl,ysPishoDfourrihneer alternatewill and feces for bAePnFoOtiwfiileldbreegdaorndeinognthteheshsiamppmleenst.ofRtehsuelts will be provided for inclusion in the final report, `Termination Unscheduled Sacrifices and Deaths Necropsies willbedone. Animals tobesacrificed will be anesthetized with ketamine and xylazine, weighed, and exsanguinated. Scheduled Sacrifices After at least 26 weeks of treatment, four animals/sex/group will be fasted overnight, then anesthetized withketamineand xylazine, weighed, exsanguinated, and`necropsied. rAefmtearinaitnlgeaasnti2m6alwseweiklls of be ftarsetaetdmeonvetrannigdhta,t least then 13 ane swteheektisze`dwiwtihtohutkterteaamtimneneta,ntdhe xylazine, weighed, exsanguinated, andnecropsied. Postmortem Procedures Necropsy `The necropsy will include examination of: al orifices cranial cavity external surfaceofthe brain; the external surface of the spinal cord and cut surfaces of the brain and spinal cord will be examined whenever tissue trimming is performed cervical tissues and organs thoracic, abdominal, and pelvic cavities and viscera external surface of the body `nasal cavity and paranasal sinuses cozs2ff Covance 325.231 -_-- 1 Papen Organ Weights pFrreosemnte)acwhilalbniemwaeliagthsecdh;edpualierdeadnodrgaunnsscwhieldlubleedwesiacgrhiefidcseesp,atrhaetefloyl:lowing organs (when adrenal (2) `brain epididymis (2) kidney (2) liver pancreas testis (2) thyroid (2) with parathyroid Organ-to-body weight percentages and organ-to-brain `weight ratios will be calculated. Sponsor. Palmitoyl CoA Oxidase Determinations "The right lateral lobe of liver will be collected from each animal at the scheduled and unscheduled sacrifices, weighed, flash-frozen in liquid nitrogen, and stored in af.reezer setto maintain -60 to -80C until dispositionofthe samples is determined by the Cell Proliferation Evaluation Representative samples of left lateral lobeofthe liver, left and right testes, and pancreas `will be collected preserved in zinc from each formalin. animal at the scheduled and unscheduled sacrifices, and eAfmtbeerdfdiexadtiionn,pasraamfpfilnesmafionrtparionleifdeartataimobniceenlltntuecmlpeearraatnutrieguennti(lPdCiNspAo)sietvioanlouafttihone wsialmlpblees is determined by the Sponsor. Bile Acid Determination All available bile (up to 5 mL) will be collected from each animal at the scheduled and unscheduled sacrifices, flash-frozen in liquid nitrogen, and stored in a freezer setto `maintain -60 to -80C. Bile samples will be packed on dry ice and shipped to: 0252 _-- Biomedical Research Centre Level 5 Ninewells Hospital and Medical School University of Dundee Dundee DD1 9SY UK Dr. Cliff Elcombe Biomedical Research Centre Ninewells Hospital and Medical School University of Dundee Dundee DD1 9SY, UK Telephone: +44 (0)1382-632641 Fax: +44 (0)1382-425586 Counce 2f9o51 Paper rReepsourltt.s of bile acid determinations analyses will be provided for inclusion inthefinal Receptor Level Determination t`hSeamspclheesdu(laeppdraonxidmuantseclhye2dugl)eodfslaicvreirfiacnesd, pancreas will flash-frozen be collected from in liquid nitrogen, each animal at and stored in a freezerset to maintain -60 to -80C. ice and shipped to: Liver and pancreas samples will be packed on dry Jon C. Cook, PhD, DABT E. 1 du Pont de Nemours & Company, Inc. Haskell Laboratory for Toxicology and Industrial Medicine Building 11 Room 1325 1090 Elkton Road Newark, Delaware 19711-0500 "Telephone No.: 302.366.5495 Facsimile No.: 302.366.5003 DuPont will be determinations wnioltlifbieedprroevgiadrdeidnfgorshiinpclmuesnitoonifnstahmeplfeisn.al Results report. of receptor level Tissue Preservation "aTnhdrereigshatmtpelsetess (taipsspureo,ixifmaantye,ly(d5ivgeidaecdhi)ntooftthhreeeliavperpraonxdiamlaltreelmyaeiqnuianlgspaamnpclreesa)swainlld bleeft collected from each animal at the scheduled and unscheduled sacrifices, weighed, flash-frozen in liquid nitrogen, and stored ina freezer set to `maintain -60 to -80C for possible future analysis. 02528 Covance 6329231 -_---m Pager1s5 "1T0he%fnoelultorwailn-gbutfifsesrueeds (fowrhmeanlipnr,esuennlte)ssfrotohmeerwaicsheasnpiemcailfiewidl,lfborepporsessiebrlveedfuitnure microscopic examination: aaodrrteanal (2) bcreaciunm ducooldonenum eespiodpihdaygmuiss (2) eyfeix[a(t2i)vetofrbeomprseacsreirfviecdedinanDiamvaildssoonnl'ys] fesmuurfrawcietohfbtohneedimsatarlreonwd)(articular ghaelalrbtladder ileum jkeijdunneuym2) lleisvieorns lung mammary gland mpeasnecnrteearsic lymph node. ppirtousittaatrey sraelcitvuarmy gland [mandibular (2)) ssceimaitnicalnevrevsei:cle (2) sskkeilnetal muscle (thigh) splinuamlbacro)rd (cervical, thoracic, and spleen sstteormnaucmh with bone marrow tessotlsut[i(2o)n tforobme sparcersiefrivceedd ainniBmoaulisnso'nly] tthhyyrmouisd (2) with parathyroid turriancahreya bladder Bone From tMhaersrteorwnuSmmoefaerach animal at unschedulaend scheduled sacrifices (made and held for possible future examination) LAisvaemrpAlePFofOliDveerte(ramnyinnaotni-ofonrmalin treated liver remaining after sampling for shaicsrtiofpicaetsh,olwoegiyg)hewdi,llfbleascho-lflreoczteendifnrloimqueiadcnhitarnoigmeanl, aatndthsetsocrhedediunleadfraenedzeurnssecthteoduled manaailnytsaisi.n -A6n0altyos-i8s0ofCulinvteirlfosrhiApPpFedOwwiitlhl sbeerduomnseaomnpltehse stoam3plMes(.KriRsesHualntssewni)llfbore provided for inclusion in the final report. c0252 Covance 6329-231 --_-- PaPaggeel1s6 Histopathology `Tissues from each animal in the control and high-dose groups and each animal that dies or is sacrificedat unscheduled interval will be embeid n pd arae ffid n, sectioned, stained `with hematoxylin and eosin, and examined.`microscopically. `The adrenals, pancrea `mid-dose groups will s, be liver, spleen, embedded in and testes from each animal paraffin, sectioned, stained in the low-and with hematoxylin and eosin, and examined microscopically. Suspected target organs noted at the high-dose will be embedded in paraffin, sectioned, stained with hematoxylin and eosin,and examined `microscopically from all animals (at the Sponsor's request and added by amendment). Reports A draft report which includes the following information will be prepared and submitted: Experimental Design and Methods Results `mortality clinical observations body weights body weight gains food consumption ophthalmic examination results clinical pathology results `hormone analyses (provided by Ani Lytics, Inc. and the DuPont Haskell Company) soerrguamn,wueriignhet,sfeces, and liver APFO levels (provided by 3M) organ-to-body weight percentages `organ-to-brain weight ratios `macroscopic observations `microscopic observations palmitoyl CoA oxidase activities (provided by the Sponsor'sdesignee) cell proliferation evaluations (provided by Pathology AssociatesInternational) bile acid determinations (provided by University of Dundee) receptor determination (provided by DuPont) cozs7 Covance 6329231 --- eagee1rr Statistical Evaluation Levene's test will be done heterogeneity of variance to test for variance homogeneity. In the atp s 0.05, transformations will be used case of to stabilize the variance. Comparison tests will take variance heterogeneiinttyo consideration. One-way analysis body weights, bod o y f variance (ANOVA) weight gains, contin will uous be cli used nical (if applicable) pathology valu to analyze initial es, palmitoyl CoA `owxilildabseeuascetidviftoiresc,onatnrdolorvgearnsuwsetirgehatteddatgar.ouIpf the ANOVA is. comparisons. significant, Dunnett's t-test One-way analysis of covariance (ANCOVA) willbe used to analyze body weights, with initial body weights as the covariate. If the ANCOVA is significant, covariate-adjusted `means will be used for control versus treated group `comparisons. Group comparisons (Groups 2 through 4 versus Group 1) will be evaluatedatthe 5.0% two-tailed probability level. Only data collected on orafterthe first day of troeradtumreinntgwrielclovbeeryanwailllyzneodt sbteataisntailcyalzleyd. Data collected statistically. before the first day of treatment At the end of 1 year after issuanceofthe audited draft report,if no requested revisions or instructions to finalize havebeen communicated by the Sponsor, then the audited draft report will be considered`final'and issued as the final report, signed by the study director, and submitted to the Sponsor. AwinllybmeodpiefrifcoartimoendsaotracddhiatnigoensaltcootshtetaoutdhieteSdpodnrasfotr.report requested 1 year after issuance Record Retention All raw data, documentation, records, protocol, specimens, and final report generated as a preesruilotdooffth1isyesatrudfyolwliollwibneg asrucbhmiivsesdiionntohfe tshteorfaignaelfraceiploirttietsootfhCeoSvpaonnscoer-.MaOdniesoyneafrorafater `submission of the final report, allofthe aforementioned materials will be sent to the Sreptoanisnoerd,ian ntdheaCroevtuarnncfeeearwcihlilvebsefcohraarngeadd.diTthioenaSlpopenrsioordomfatyimelee,ctantod hCaovveatnhceemwaitlelcrhiaalrsge astorage fee. If the Sponsor chooses to have Covance dispose of the materials, a disposal fee will be charged. All raw data stored on `magnetic media will be retained by Covance. Within oneyearafter submission of the final report, all of the aforementioned materials from the Sponsor's designees (Ani Lytics Inc., E. 1. du Pont de Nemours & Company, Inc, SME.T. & 8, Pathology Associates International, and University of Dundee) will be sent to the Sponsor (Paul Lieder, PhD, DABT, 3M) to be maintained. conAsg _-- -- PROTOCOL APPROVAL Covance 632931 PPaaggeess Pod Lid Paul Lieder, PhD Diplomate, ABT Study Monitor 3M TT erm David Farrar, PhD ICI Chemicals & Polymers Limited # I ZsPhD Z Study Director Department of Toxicology Covance Laboratories Inc. Yes/zp Date _ Date Date ;> cc2538