Document 5bYjbNyV655XQpx3xgRzyRGgR

WARF INSTITUTE, INC. MADISON, WISCONSIN Reports are submitted to clients on a confidential basis. No reference to the work, the results or to the Institute in any form of advertising, news release or other public announcement may be made without written authorization from the Institute. REPORT Sample: T-1166 WARF No. 5011023 Date Received: 1-13-75 Submitted by: J . E . Long 3M Company St. Paul, Minnesota Testing requested: Skin Irritation Eye Irritation Conclusion: Under the conditions specified the product has a primary skin irritation score of zero and eye irritation scores of 23.67, 23.67, 21.67, 13.50 and 3.33 at 1, 24, 48, 72 hours and seven days respectively for unwashed eyes. The product is not irritating to the skin and is irritating to the eye. Report approved by: Signed and for WARF Institute, Inc, Date: January 28, 1975 _______ ____ ,<Z Donald L. Hatris/^D.V.M/' Chief Pathologist/Toxicologist FORM 36-4 001735 W WARF INSTITUTE, INC. MADISON, WISCONSIN Sample: T-1166 WARF No. 5011023 Primary Skin Irritation: Method: Albino rabbits were used for this work. They were housed in individual screen bottom cages and fed water and rabbit chow ad libitum. The hair was clipped from the back and flanks of the animal. Five tenths of one milliliter of test material was placed in intimate contact with the skin on prepared test sites (abraded and intact), and covered with gauze patch and tape to maintain contact with the skin. After a 24 hour contact period, the coverings were removed and the degree of erythema and edema was recorded according to the scale below. Readings were taken again at 72 hours and the 24 and 72 hour readings used to determine the primary skin irritation score. Results: Animal Number _____24 Hours_____ Abraded Unabraded _____ 72 Hours_____ Abraded Unabraded Average 1 2 3 no noted irritation 4 all sites read negative 5 6 Primary skin irritation score: 0 Scale for Evaluation of Skin Reaction Erythema and Eschar Formation Score Edema Formation Score Slight erythema Defined erythema Moderate to severe erythema Severe erythema to slight eschar formation 1 Slight edema (barely 2 perceptible) 1 3 Defined edema (edges definite rising) 2 4 Moderate edema (area raised 1 mm) 3 Severe edema (raised more than 1 mm) 4 Score equals sum of erythema and edema readings. Skin irritation index equals average of 24 and 72 hour scores. FORM 28-4 0017S6 H WARF INSTITUTE, INC. MADISON, WISCONSIN Sample: T-1166 WARF No. 5011023 Eye Irritation: Method : Adult albino rabbits of the New Zealand White variety were placed in a collar such that the animals could not rub their eyes. One-tenth of a milliliter (0.1 gm for solids) of test substance was instilled in one eye, the other untreated eye served as a control. A series of six albino rabbits was used for each substance. The reaction to the test material was read according to the scale of scoring for damage to the cornea, iris and the bulbar and palpebral conjunctivae at 1, 24, 48, 72 hours and seven days after eye instillation. Any residue of the test material and accumulated discharge are flushed from the eye each time they are scored. Administration : Concentration of test sample : as submitted Diluent or solvent: none Special washing: none Results : Rabbit Cornea Number Opacity Area Iris Conjunctivae Redness Chemosis Discharge 1 140 2 2 2 2 120 2 2 1 13 120 2 2 2 hour 4 120 2 3 2 5 000 2 2 2 6 220 2 2 2 1 hour eye irritation score: 23.67 1 140 2 2 2 2 120 2 2 2 24 3 120 2 2 2 hours 4 120 2 2 2 5 000 2 2 2 6 220 2 2 2 24 hour eye irritation score: 23.67 48 hours 1 2 3 4 5 6 140 120 120 220 000 220 2 2 2 2 2 2 48 hour eye irritation score: 1 1 1 1 1 1 21.67 1 1 2 1 1 1 0017S7 FO RM 28-4 H WARF INSTITUTE, INC. MADISON, WISCONSIN Sample: T-1166 Eye Irritation: (continued) WARF No. 5011023 Results : Rabbit Number 1 2 72 3 hours. 4 5 6 7 days 1 2 3 4 5 6 Cornea Opacity Area Iris Conjunctivae Redness Chemosis Discharge 120 2 0 0 120 2 0 0 110 2 0 1 120 2 0 0 000 2 0 0 220 2 0 0 72 hour eye irritation score : 13.50 000 0 0 0 000 0 0 0 000 2 1 2 000 0 0 0 000 0 0 0 120 0 0 0 7 day eye irritation score: 3.33 FORM 28-4 0017SS W WARF INSTITUTE, INC. MADISON, WISCONSIN Reports ore submitted to clients on a confidential basis. No reference to the work, the results or to the Institute in any forr REPORTof advertising, news release or other public announcement may be made without written authorization from the Institute Sample: T-1166 WARF No. 5011023 Date Received: 1-13-75 Submitted by: J . E . Long 3M Company St. Paul, Minnesota Testing requested: Eye Irritation Conclusion: Under the conditions specified the product has eye irritation scores of 7.33, 6.67, 6.00, 2.67 and zero at 1, 24, 48, 72 hours and seven days respectively for eyes washed after a five second exposure and eye irritation scores of 8.67, 11.33, 7.33, 4.67 and zero at 1, 24, 48, 72 hours and seven days respectively for eyes washed after a 30 second exposure. The product is irritating to the eye. Report approved by: Donald L. Harris,^D.V.M. Chief Pathologist/Toxicologist FORM 36-4 01759 Sample: W T-1166 WARF INSTITUTE, INC. MADISON, WISCONSIN WARF No. 5011023 Eye Irritation: Method: Adult albino rabbits of the New Zealand White variety were placed in a collar such that the animals could not rub their eyes. One-tenth of a milliliter (0.1 gm for solids) of test substance was instilled in one eye, the other untreated eye served as a control. A series of three albino rabbits was used for each substance. The reaction to the test material was read according to the scale of scoring for damage to the cornea, iris and the bulbar and palpebral conjunctivae at 1, 24, 48, 72 hours and seven days after eye instillation. Any residue of the test material and accumulated discharge are flushed from the eye each time they are scored. Administration : Concentration of test sample: as submitted Diluent or solvent: none Special washing: eyes washed with 300 ml. of distilled water over a two minute period, after a five second exposure. Results : Rabbit Cornea Number Opacity Area Iris _______ Conjunctivae_________ Redness Chemosis Discharge 1 0 00 1 0 1 12 0 00 1 2 1 hour 3 0 00 2 2 1 1 hour eye irritation score: 7.33 1 0 00 2 0 0 24 2 000 2 1 1 hours 3 0 00 2 1 1 24 hour eye irritation score: 6.67 1 000 2 0 1 48 2 000 2 1 0 hours 3 00 0 2 1 0 48 hour eye irritation score: 6.00 1 00 0 2 0 0 72 2 00 0 1 0 0 hours 3 00 0 1 0 0 72 hour eye irritation score: 2.67 001790 FO RM 28-4 H WARF INSTITUTE, INC. M A D I S O N, W I S C O N S I N Sample: T-1166 Eye Irritation: (continued) WARF No. 5011023 Results : Rabbit Number 1 72 days 3 Cornea Opacity Area Iris _______ Conjunctivae_________ Redness Chemosis Discharge no noted irritation all readings negative 7 day eye irritation score: 0 FO R M 26*4 001*791 M T WARF INSTITUTE, INC. MADISON. WISCONSIN Sample: T-1166 WARF No. 5011023 Eye Irritation: Method: Adult albino rabbits of the New Zealand White variety were placed in a collar such that the animals could not rub their eyes. One-tenth of a milliliter (0.1 gm for solids) of test substance was instilled in one eye, the other untreated eye served as a control. A series of three albino rabbits was used for each substance. The reaction to the test material was read according to the scale of scoring for damage to the cornea, iris and the bulbar and palpebral conjunctivae at 1, 24, 48, 72 hours and seven days after eye instillation. Any residue of the test material and accumulated discharge are flushed from the eye each time they are scored. Administration: Concentration of test sample: as submitted Diluent or solvent: none Special washing: eyes washed with 300 ml. of distilled water over a two minute period, after a 30 second exposure. Results: Rabbit Cornea Number Opacity Area Iris Conjunctivae Redness Chemosis Discharge 1 000 1 2 1 12 000 1 2 1 hour 3 000 2 2 1 1 24 2 hours 3 1 hour eye irritation score: 8.67 000 000 000 2 2 3 1 2 2 1 2 2 24 hour eye irritation score: 11.33 1 000 2 1 0 48 2 000 2 1 0 hours 3 000 3 1 1 1 72 2 hours 3 48 hour eye irritation score: 7.33 000 000 000 2 2 2 0 0 1 0 0 0 72 hour eye irritation score: 4.67 001792 FORM 28-4 U WARF INSTITUTE, INC. MAD ISON, W I S C O N S I N Sample: T-1166 Eye Irritation: (continued) WARF No. 5011023 Results: Rabbit Number 7 days 1 2 3 Cornea____ Opacity Area Iris _______ Conjunctivae_________ Redness Chemosis Discharge no noted irritation all readings negative 7 day eye irritation score: 0 FORM 28-4 001792