Document 5bJDr6YEZJzazXkODNJdmjLG5

TOXICITYTO AQUATIC PLANTS TEST SUBSTANCE IdentitAy:mixturceontainipnegrfluorooctanesulwfhoincahtmea,y alsobe referredtoas PFOS, FC-95,oras a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted.The testsample isFC-203. Currentinformatioinndicateistisa mixtureof1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol,2.66 % Sultonefoamer,3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately retlecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: OECD Guideline201 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrumcapricomutum Source: In-houseculturesO.riginalolbytainedfromtheCultureCollectioonf Algae atthe UniversitoyfTexas atAustin. Element basis: Algalcellcounts(cellsimig)r,owth rates. Exposure period: 96 hours StatisticaMlethods: The EC50 valueswere calculatedusingthe computer program ofStephan,1983. The NOEC was calculatedusingDunneft'stest. Analyticalmonitoring: pH and temperature. Test Conditions: Algal NutrientMedium: Nutrientmedium per U.S. EPA, 1978. This nutrient medium providedallmineralnutrientesssentiafloralgalgrowth and also served as thediluenftorallalgaloperations.The pH ofthissynthetiaclgal medium was -8.0. Stock and testsolutionpreparation:A 1000 mg/L primarystocksolution was preparedinnutrienmtedium. Aliquotswere then added directltyothe testvesselsto createtestsolutions. Exposure vessels: 250 ml Erienmeyerscontaining100 ml testsolutionand capped withinvertedglassbeakers. Agitation:Shaken continuouslayt 100 rpm. Number ofreplicate3s: Initicaellloading1:.0 X 104 cells/mL Number of concentrations: fiveplusa blankcontrol Lighting:35 pE!n/seC/M2from continuouscool-whitefluorescenltighting. Water Chemistry: pH range (0-96hours): 7.8- 9.4(controelxposure) 7.8- 8.0 (1000 mg/L exposure) Test temperature range (0-96hours): 23.3- 23.4OC (incubator) RESULTS Nominal concentrations:Blank control6,2.5,125,250,500, and 1000 mg/L 96-Hour Algal Growth Response Values Nominal Conc. (mg/L) Control 62.5 125 250 500 1000 Mean Cell-Count (cells/mL) 399,000 645,000 347,000 69,000 51,000 12,000 Average Specific Growth Rate 0.038 0.044 0.037 0.020 0.017 0.002 Percent Change in Growth Rate from Control (96 hrs.) -- -16 3 47 55 95 Algal Growth Response ErCrovalues: 96-hour ErC50= 354 (323 - 389) mg/L 96-hourNOEC = 500 mg/L Element valuesarebased on nominalconcentrations. Reversibilitoyf Growth InhibitionN:ot give. Remarks: Testingwas conductedon the mixtureas describedintheTest Substance Remarks fieldT.he valuesreportedapplytothatmixtureand not the fluorochemicaplroportioanlone. CONCLUSIONS The testsubstanceexhibitas 96-hourErC50growth ratevalueof354 (323389) mg/L. The 96-hourNo Observed EffectConcentration(NOEC) is500 mg/L forgrowth rate. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.This studymeets thecriterifaorquality testing.However, sample puritwyas notpropedy characterizeadnd the study lacksanalyticaclonfirmatioonfthe amount offluorochemicaplroportioninthe solution. REFERENCES Thistestwas conductedby EnviroSystemsDivisionR,esource Analysts,Inc., or Hampton, NH attherequestofthe3M Company, Lab Request number H2864-9,1991. OTHER Last changed: 6/27/00 Study Title Static Acute Toxicity of rC 203 to the Alga, Selenastrum capricornutus Autbors Robert L. B(>eri Tinotby J. Ward Study Completed June, 1991 Performina Laboratory Eiiir*o'SystessDivision Rosource Inalystsi Incorporated P.O.@ Box 2130 @4 Oike,Lafalette Road@. @.'imptongl*ew, N"pabire 03942,.@ pigi @'1'of' 15 I. SUMMY The acute, toxicity of PC 203 to the alga, Selenastruz capricornutus, is described in this final report. The test was conducted for 3N- Company for 96 hours during Way 6 to 10, 1991, at the EnviroSysteas Division of Resource knalysts, Inc. in Hampton, New Hampshire. It was conducted by Jeanne Magazu, Peter Koval.ski,Ellen Stanford, Robert Roeri, and Timothy Ward. The te@E was performed under static conditions with five concentrations of test substance and a dilution water control at a mean temperature of 23.60C. The dilution water was synthetic media prepared with sterile deionized water. Flasks were incubated on a rotary sbater. Light was adjusted to 35 nEin/sec/sk2with a photoperiod of 24 hours light and 0 hours dark. Nominal concentrations of FC 203 were: 0 mg/L (control), 62.5 mg/L. 125 mg/L, 250 mg/L, 500 ngfl. and 1,000 mg/L. Nominal concentrations were used for all calculations. Algae used in the test was from an in-boozeculture that was maintained under test conditions. Exposure of algae to the test substance resulted in a 72 boor BC50 of 339 mg/L rC 203 and a no observed effect concentration (NOEC) of 250 agfl. The 96 hour ECSO and NOEC are 354 mg/L and 500 mg/L, respectively. 2 '6f M*ft kir@&kddm d MUJPORE,:'.-- I'l. TILBLZ OF CDNTMM SECTION: 1. Sumary ii. Table of Contents III. Index of Tables IV. Introduction V. Metbods and Materials Vi. Results VII. References Appendix A. gater Quality Data from Toxicity Test PAGE 2 3 4 5 5 8 12 13 Page,3 of 15 kx*Vft kr- &A)ddkirdy IOLUPORE TIT. MEX OF TIBIM Table 1. Cbenical characterization of a representative sample of deionized water used to prepare test media for toxicity test Table 2. Growth data from toxicity test Table 3. Percent change from control and average specific growth rate from toxicity test Table 4. Median effective concentrations (EC50s) from toxicity test Table A.l. Temperature sealzured during toxicity 'lest Table A.2. pH measured during toxicity test PAGE 6 9 10 11 14 is Page 4 'of 15 'IC*VlitrtV'Ski@@ 0 1.4FiLJFIM rV. INNODUCTION This study was sponsored by 3M Company, St. Paul, Minnesota. The objective of the study Was to determine the acute toxicity of FC 203 to a freshwater alga. The report contains sections that describe the methods and materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test- V. EMODS AND NTERIALS TEST SUBSTANCE: rC 203 (EnviroSystems Sample Number 2699E) was delivered to EnviroSysteas on lgoveaber 15, 1990. It was contained in a 250 aL glass bottle that was labelled with the following information: "H 2864-9, FC 203". FC 203 (a clear aaber liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately1.1 grass) will be arcbived at EnviroSystess for a minimum of 10 years. DILUTION WATER: Water usid for acclimationof test organismsand for all toxicity testing was sterile enriched media (U.S. EPA, 1978) with a pE of approximately 8.0. Results of chemical analysis of a representative sample of deionized water used to prepare the media are presented in Table 1.* TEST ORGANISM: Algae used for the test was from a culture originally procured from the Culture Collection',-..Aolfgae at the University of Texas at Austin and delivered @to Envirosysteas on oril -12.'1990.-The identity of the culture .-.was.,,.verified-ln'gu.sian.-,appropriate- taxonomic key. The culture was '-tratisfarred,.to,-ster@i'el@neriched ."iedLa,-i,dentical to"@edia used for this test_and maintained at test-conditions.' xiciTT,- STING:,. '-.The-d,efinitive toxicity"t@ii@,@as performed during April 22 to*'26, @9 it-.i,s ,base-I on pr"ureii @k@f tbe@'OECD (1994). The test.was conducted ,"at:.'a'target@,tm'perature of. "22 ''2*C with five concentrations of f;*t-est substance and-a dilutioilw.ater. control.-" 1,000 mg/L stock solution was,, prepared@-in?dilation v tei:*'@,,.Thsetock solution was added directly to "-dilUtion'rwater -contained -in the' teLst@-,,-,vessewlisthout the use of a solvent.@,i",N,o-minal' concentrations@,,of@'@@,the-,@@test material were: 0 mg/L (control),62.5 mg/L,@-12S. mg/L,--250 '09/L"',500 "ii4L/, and.1,000 mg/L. Stbddksy,rimLum-,wt Table 1. Chemical characterization of a representative sample of deionized water used to prepare test media for toxicity test Parameter Unit of Measurement Reporting Limit Measured Value Organochlorine ug/L 2 ND pesticides Organophosphorus ug/L 0.5 ND pesticides Polychlorinated ugfl O-S ND biphenyls Note: ND not detected at or above the reporting limit. Page 6 of 15 Resm"AzcWLbv-SubddioyCfMtLI10M Algae was randomly and equally distributed among three replicates of each treatment at the rate of 10,000 cells/al. The test was performed in 250 al glass E-rlenneyer flasks that contained 100 ol of test solution. -he flasks were capped with inverted glass beakers. Test vessels were randomly arranged on a rotary shaker in an incubator during the 96 hour test (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was maintained with cool-white fluorescent lights that provided a light intensity of 35 uEs-lor2. The number of algal cellslal in each test vessel was determined visually every 24 hours by means of direct microscopic counts with a benacytometer. The pH (Becbm model pEl 12 meter) was determined in each test flask at the beginning and end of the test, and temperature (ASTM mercury tbermmeter) was measured and recorded daily in the incubator. STATISTIC)LL NMODS: Results of the statistical techniques toxicity (Stepban, test were interpreted by standard 1983). The binomial or moving average method was used by the author to calculate ECSO values using nominal concentrations of Dunnett's test, variance (AMOVA). the natural log test which substance. includes a The NOBC parametric was calculated using one-way analysis of The average specific growth rate was calculated as of the number of cells per al at 96 hours minus the natural log of the number of cells per al at 0 hours divided by the exposure period. subtracting the average specific specific growth The percent change from control is calculated by sample average specific growth rate from the control growth'-rate, dividing that value by the average rate in the control and multiplying that value by 100. The percent values. change from control 'was used to compute ECSO and NOEC ' iz, Page 7 of'ls QowLvm ArcW% bir-&A)Aclady MUSKX@E Vi. RESULTS Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A,-respectively, and the percent of control and average specific growth rate information is presented in Table 3. The 24, 48, 72, and 96 hour EC50s for algae ezposed to rC 203 are presented in Table 4. The 72 hour EC50 is 338 mg/L, and the 72 bour NOEC is 250 mg/L FC 203. The 96 hour ECSO is 354 ngfl and the 96 hour NOEC is 500 mg/L. Page 8 of 15 7 Resotime Anciysts.ky- 9jbddkxy ai Table 2. Growtb data from toxicity test Nominal Concentration fog/L) rep. 0.0 (control) 62.5 125 250 Soo I ooo 1 2 3 mean 1 2 3 mea.n 1 2 3 mean 1 2 3 mean 1 2 3 mean 1 2 3 mean Number of Cells per Milliliterx 102 (hour) 0 24 48 72 96 10 26 94 10 32 34 10 30 116 10 29 81 10 30 92 10 52 94 10 48 92 10 43 93 10 26 52 10 26 76 10 34 46 10 29 59 10 28 48 10 26 62 10 20 42 10 25 51 10 32 32 10 28 10 28 64 10@- 35 41 10 ^4 36 10 20 36 10 26 32 23 35 188 394 172 419 282 386 214 399 346 604 256 596 296 734 299 645 100 350 82 214 94 476 92 347 62 76 42 82 64 so 56 69 @30 58 23 sa 26 36 28 51 28 24 30 14 27, 12 Page' 9 of Re" rceAnr@I Table 3. Percent change from control and average specific growth rate from toxicity test Nominal Concentration of Test Substance (mg/L) Average specific grovtb rate 24hr 49hr 72hr 96hr 0.0 (control) 62.5 125 250 Soo 1,000 0.04S 0.044 0.043 0.039 0.062 0.047 0.047 0.044 0.044 0-037 0.031 0.037 0.038 0.034 0.024 0.020 0.052 0.030 0.014 0.017 0.036 0.026 0.014 0.002 Percent change from control 24hr 49br 72br 96hr 0 0 0 0 -39 -7 -9 -16 2 16 28 3 16 23 44 47 -16 32 67 55 20 41 67 95 7 p age!-.1.u Table 4. Median effective concentrations (EC50s) from toxicity test Exposure period ECSO 95 percent confidence limits Calculation method 24 hours 48 hours 72 hours 96 hours )1,000 mg/L )1,000 mg/L 338 mg/L 354 mg/L 293 - 395 ag/L 323 - 389 mg/L moving average moving average Pace 11 of 15 VII. RERERENCES OECD. 1984. Guidelines for Testing of Cbesicals. Section 2: Effects on Biotic Systems. Metbod 201, Alga, Growtb Inbibition Test. Adopted June 4, 1984. Stepban, C.E. 1983. Computer program for calculation of LC50 values. Personal communication. U.S. EPA. 1978. The Selenastz-un capricornutuz Bottle Test. EPA-60019-78-018. July, 1978. Printz Algal Assay 41 Page 12 of 15 " i7@ pm mh ine,SiibirlcrfdivllLlvm Appendix A. WATER QUALTTY DATA RROM TOXTCTTY TEST -JK' Page 13 of, 25 Table A.I. Temperature measured duridg toxicity test Day of Exposure 0 1 2 3 4 Temperature of Incubator,QC 23.3 23-3 23.3 23.4 23.4 Page 14 o f ls * RSWLKO Table A.2. pH measured during toxicity test Nominal concentration (mg/L) 0 (control) 62.5 125 250 500 1,000 Replicate 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 PH initial final 7.9 8.0 7.8 8.0 7.8 9.4 7.7 9.5 7.7 10.0 7.7 9.5 7.7 8.7 7.7 8.7 7.7 3.7 7.7 3.3 7.7 8.3 7.7 8.3 7.6 9.3 7.7 8.2 7.7 8.1 7.8 8.0 7.9 9.0 7.8 8.0 16 Page 15 of AL- ' Vf ResounAenok-fktr-!kjtmi:dfMtIyLJSIOK 7