Document 5b6eJZ0KByaENJ757QXgYjn6R

A236 - 3283 465 TRADE SECRET ARAR6 ~3283 Dupont 12091 Study Tite H-25767R:aiSntabtoiw, TArcouutte,,O9n6c-oHrohuyrncShcruesemnyiknigsTsest with AUTHORS:RoBbarebrrtaAD.. FHeorkree,l,PBhSD.. _ STUDY CompLETED ON: uly 1, 2003 PERFORMING LABORATORY: EH.aLskedlulPLoanbtodreatNoerymofuorrsHeaanltdhCaonmdpEannvyironmental Sciences ENelwiaornk,RoDaedl,aPw.aOr.e B1o9x71540-0050 `WORK REQUEST woveJRL STupY Cope EE a) -- : `Company Saniized. Doss not contain TSCA CB! TT ihRainbow Tta ,Oncor--mS:-- ps CERTIFICATION Dwron12991 `We, the undersigned, declare that this report provides an accurate evaluationofdata obtained from this study. WorkCompleted by: PakBaarb2a..VrehAei 595 ] ettSy Dito ABTob4eP. ASrkeFED. SerioReseBese sodManager a 203 L Tay T 223 Pe CompanySanitized.Doesnotcontain: TSCACBI s rr ---- per-- ieos-- rearsatee 1it2h57R6ai7nboSwacT,nA,cutOe, 9c6-HooureSreneing Ts STUDY INFORMATION suserecs IIR Synonyms/Codes: H-25767 a Haskell Number: 25767 Compositior | Duron i2001 Soon lnten Physical Characteristics: White liquid Stability: The test substance appeared to be stable under the conditionsofthe study. - Sponsor: E.L du Pont de NemoursandCompany Wilmington, Delaware 19898 US.A. Study Initiated/Completed: May 12, 2003 / (see report cover page) Company Santized Dossnot contain TSCA Cot ------------ _ vHii-t2hhSR7R6aa7ii:nnSbbtooawwtTiTrcrooAuuc,,utOOenn,cco9or6rh-hyHymoncuchrhuuSssmcmpyrbkieiesnssiTnesgt DDuuPpoonmti1o2g9n91t SUMMARY `The acute unaerated, toxicityof H-25767 96-hour, static test. to the rainbow trout, Oncorhynchus mykiss, was determined in an ``mTheeansttuedmypewraastucroenodfuc1t2e.d8wiCt(hr4ancgoencoefn1t2r.a7t-i1o3n.s0ofC)H.-2O5n7e6t7esatncdhaamdbileurtwioanswautseerdcpoenrtrtoelstat a `cwoantceernctornattrioolnawnidth1 1a0ntdes1t0ormgga/nListemsst cionnecaecnhtrcahtaimonbesro.lutBiaosnesdwoenrveicslueaalr aobnsdercvoaltoirolness,s atthetedsitlsuttairotn The `The 100 mg/L test concentration solution was slightly cloudy 1000 mg/L test concentration solution was cloudy with a with a surface surface film a film test at test stat, start. All water quality parameters were within acceptable limits during the exposure. E1,xp1o0,su1r0e0o,fanrdai1n0b0o0wmtgro/uLt troestuhleteddiliunt0io,n0,wa0t,e0r,caonndtr1ol0a0n%dmnoortmailniatyl,Hr-es2p5e7c6ti7vecloyn,ceanttrhaetieonndsooff 9or6ghaonuirssm.s.NoThmeorhiaglhietsytonrosmuibnlaelthcaolnecfefnetcrtastiwoenrecasueseinnginntohemodritlaultiitoynawtatteesrt ceonndtrwoalste1st00 mg/L. `HT-h2e5l7o6w7esctonncoemnitnraalticoonnscwenetrreatuisoendcfaourscianlgcu1l0at0i%onmoorftLaClityvaatluteesst.end was 1000 mg/L. Nominal The results are summarized as follows: ~~ Nominal concentrationsof H-25767, mg/L| dilution water control, 1, 10, 100, and 1000 96-hour LCso (95% confidence interval) for H-25767, based on nominal | 316 (100 to 1000) concentrations, mg/L" uHs-i2n5g7t6h7e erxahiinbbiotwedtrlooutw,cOonnccoerrhyfnocrhauqusamtyikcishsa.zard" in an unaerated, 96-hour, static acute test PSrtoepcheaend,inCgEs.of1t9h9e7)Fi.rsMteAtnhnoudasloSrymcpoasliulmionnsgAnquLaCtsi.c TAogxuiactoilcogTyo,xAicSoTloMgySapnecdiaHlaPzuabrldiEcavtailounat6i3o4n,(FL. Mayer b aSmnrdcJhLe.k,HaJm,eCllienmke,ntEsd,sR,,pMo6r5c-o84c.k,PRh.ilaanddelRpahbieart, W. (1993). Assessing ecological hazard under TSCA: ~ WPmhe.itGlha.oddLesapihnidd.isevLaSl.uaHtuigonhoesf,daaan.d ME.nAv.irLoemwesn,taEdlsT)o,xipcpo2i2og-y3a.ndAmReirsikcAasnseSsoscmieentty,fAorSTTeMstiSnTgPan1d17M5i,aterils, ee ee ---- Company Comoe Saniized. Doss not contain TSCA Cl Pov mt cong ran 2vhHiAt2hSR7Ra6a7ii:nnbboSotwwaTtTerr,oouAtce,uOtOenn,coo9r6rHllyoynunccrhhuuSscsmrmeybekeniissnsg Test DDuuoPmoneti1o29s9n1 MATERIALS AND METHODS A. Test Solution Preparation nTeosmtinsaulbsctoanncceentsroaltuitoinonssofwteersetpsruebpsatraendcebysodliurteicotnsadwdeirtieonnootfHad-j2u5st7e6d7fotro pduirliuttyi.onTweastters.oluTthieons c`woenrteaipnreedpa1r0edLobfy HaadsdkinegllthLeabaoprparotporriyawteelalmwoautnetroafntdessttisrruibnsgtafonrceaptporcoaxcihmattesetlych4a4mmbienrutwehsi.chThe tdeisltutsitaornt.waTtheer c1o0n0trmogl/aLndte1stacnodnc1e0nmtrga/tiLontesstolcuotnicoenntwraastisolnigshotlluytcilonosudwyewrietchlaeasruarnfadcceofliolrmleaststaetst start. The 1000mg/L test concentration solution was cloudy with a surface film at test start. B. Dilution Water aDnildusteiaolnewdattoerbeodrriogcikn.atTehd efhraormdtnheesHsaosfktehlleLwaeblolrwaattoerry wisealdjwuhsitecdhtios a3p78p-rfoexeitmadteeelpyand is cased 100-140 aerated, mg/L as CaCO; passed through by the green sfalnodw-fproipotroltrieoenmeodrvaeddiirtoni,onaonfdCfailCtler.ed Tthhreowueglhl5w0-a,te1r0-i,s then and d0i.s4t5ri-buumtefdilttherrsotuoghreamgoedvepoplayrtviicnuyllatcesh.lorTihdeepwiaptienrg.isThheeatdeidluotriocnhiwlaltederasisaapnparloyprzieadttewaincde yearly ~ for major anions and cations, metals, total organochlorine and organophosphate pesticides, and `sppeocliyfcihclaotriionnast.ed biphenyls. The dilution water meets OECD" and ASTM criteria and C. Test Organism Culture CRaaliifnobroniwa.troTuth,eOfnicshorwheyrnechheulsdmiynkais2s7,2w-Le,reciprucrulcahra,sfeidbefrrgolamssThhoolmdaisngFitsahnkCo(8m4p-acnmy,diAanmedteerrs,on4,9cdamydpeeprtiho)dfporrec1e8ddianygstihne cteosntt,iwnautoeursltye-mfpleorwaitnugrewselrlanwgaetderfartomHa1s0k.e4lltoLa1b1o.r2atCor(ym.eaDnur1i0n.g9tCh)e.7`The fish were fed freshly-hatched, live brineshrimp (San Francisco Bay Brand,Inc.)and A`wqeueakdMaayxsanSdtaorntecreFdianigleyrloinngwe3e0k0eSndDs03an(dPMhoIlidNauyst.ritSiiocnkInnetsesr,naitnijounrayl,,aLnLdCv)istiwbileceabdnaoirlmyaolnities wduerrienngotthepr4e8sehnoturins fpirsihorintothuesheoflodritnegsttianng.k nFoirshinwtehreefiisdhenutsifeidedfobrytelsatbienlgs.oMnotrhtealhiotlydwinagst<an1k%s and test chambers. D. Test Methods Fnoomuirnnaolmitensatlcotnesctenctornacteinotnrsatwieornse a1,nd10a,d1i0l0u,tiaonndwa1t0e0r0cmongi/rLolHw-e25r7e6u7s.ed in this study. The _--_ mm ---`Company Saniized. Doesnot contain TSCA CB} 2h1wi2t5hR7Ra6ai7in:nbbooSwwiTcTrr,oouAutc,u,tOOenn,cc9oo6rr-blHyopnucnrhhiSuscszmmcykkniiissnsgs Test DDuuPoomnitz1e29g91n ~ a"Tpepsrtocxhiammabteelrys w1e0rLeosfttaeitnssolsutteieolna(q2u6ar-iLam(a3x0i(lmeungmthv)olxu3m0e;(w1i1d.t5h-)cxm 3te0st(hseoilguhtti)oncrdne]ptwhh)i.chOnhee.ld Eatecsthcchhaamwwambasusebsceoredveprreerdtewsittshoalugtliaosns(ptoltaatl otfo$prteevsetncthtahmebefrissh;f1r0omfiesshcianpienagc.h test chamber). c`Aodmdpilteitoendo.ffMiosrhatloitthyeantedstbseohlauvtiioornaslwoabsseirtvaattieondsafwteerremmixaidnegeovetrfhy 2t4eshtosuorlsutainodnsatwatesst end. wDeeraedtfhieshawbseernecreoemfoovpeedrcfurloamr mthoevteemstencthaamnbderlsacdkourfinrgeadcatiloynotbosegrevnattlieonpsr.oddCirnigt.eriAatfofresdteath conclusion, all fish were humanely sacrificed using MS222 (3-aminobenzoic acid ethyl ester). Athere9c6i-rhcouluarttiensgtwaattaeprpbraotxhimwaatseluyse1d2.t8omCaiwnittahiarnamnegaen otfem1p2er7attour1e3.i0ntCh.eAtestphcohtaompbeerrisodduorfing i1n6chlouduerds 3li0ghmti(nuptpersooxfimtartanesliyti2o2n3altloi4gh8t4(L52uxt)o a1n9d8 8Luhxo)urpsredcaerdkinnegssawnadsfeomllpolwoiynegdthweh1i6c-hhour light interval, Dmiesassoulrveemdenotxsygweenrecotnackeenntrbaetfioorne, pfiHs,h awenrdeteamdpdeerdatautrteeswtesrtaertm,evaesurrye2d4ihnoaulrstetshtecrehaafmibcer,rsa.ndTahtese mteasntneendr. aTeesstt esonldu.tions were not aerated during the test and were disposedofin an appropriate ~~ RESULTS AND DISCUSSION E1x00p,osaunrdeo1f00r0amingb/oLwrtersouultteod itnhe0,w0a,te0r, c0o,natnrdol1a0n0d%nomomritnaallitHy,-2r5es7p6e7ctciovenlcye,natratthieonesndofof1, 10, o9r6ghaonuirssm.s.NTohmeorhiaglhietsytonromsuibnlaelthcaolncefefnetcrtastwioenrecasueseinnginntohemodritlaultiitoyn awtatteesrtceonndtrwoalste1st00 mg/L. `HT-h2e5l7o6w7esctonncoemnitnraalticoonnscwenetrreatuisoendcfaourscianlgcu1l0at0i%onmoofrLaClistoyvaatluteesst.end was 1000 mg/L. Nominal The 96-hour LCso for H-25767 to rainbow trout was 316 mg/L. REFERENCES 1. TOregsatniinsgaotfiCohnefmoircEalcso:no2m0i3c,C1o7-JOulpye1r9a92t.ianodn Development (OECD). Guideline for the 2. AComnedruicctainngSoAcciuettye fToorxTiecsittyinTgesatnsdwMiattherFiiaslhses(,AMSaTcMr)oi.nve(r1t9e8b8r).ateSst,anadnadrdAmGpuhiidbeiafnors. E 729-880. Annual Book ofASTM Standards, Vol. 11.04, Ae ------ ee tteeee--e~ene Company Saniized. Doesnotcontain TSCA C=! wHiSth76Ra7i:nbSotwTicr,ouAc,utOen,c9o6rlHyonucrhiSscrmeyekniisnsg Test Dupont12991 TABLE1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal Test Initiation (0 hours) Test Completion (96 hours) ConHc2en5t7r6at7ion|| Dissolved Oxygen DOisxsyoglevned (mg 2 p (mg p Water Control| 10 10.7 107 10 107 77 77 8991 77 90 7733 100 1000 10.7 108 77 88 73 73 77 102 74 2 Messurements taken a1 24 hours due to total mortality in tet concentration, TABLE2 - MORTALITY OF Oncorhynchus myTkiEsSsTINWIATNHUHN-A2E57R6A7TED, STATIC, ACUTE, 96-HOUR Nominal ConcHe2n5t7ra6t7ion mg `Water Control 10 10 100 1000 [2Hows 10 010 010 10 10/10 NumbNeurmabteTresDteIandit/iation a8Hows | 72Hows| 96Hows | oo 010 10 010 10 10 10 10 10 ono 001100 10710 1010 10710 ~ -Y `Company Sanitized. Does notcontain TSCA Ce"