Document 5OdLLoJZXpBQ50wDda8JynVz
A k A S .6 - O i& l
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE____________________________________________
Identity: A mixture containing periluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603F. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 501. The test sample is FC603F. Current information indicates it is a mixture of 0.5% PFOS, 7% diethylene glycol butyl ether, 82.95% water, 2% acrylic foamer, 4% Alkylether sulfate, 1.45% Xanthan gum, 1.4% calcium acetate, 0.07% N-(3Chloroallyl)hexaminium chloride, 0.5% triethanolamine, 0.05% sodium hydroxide, 0.03% cis-1-(3-Chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride, and 0.05% tolytriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD____________________________________________________
Method: OECD Guideline 209 GLP: No Year Completed: 1994 A nalytical m onitoring: Dissolved oxygen concentrations, temperature, and pH. S tatistical m ethods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN. Test condition:
D ilution water: Millipore Milli-QTM water Synthetic sewage: per OECD method Reference and test solutio n preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2SO4 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.5. Test substance was added directly to the test vessels. Test vessels: Not given. Number o f concentrations: one and a blank control, in duplicate, plus 1 reference substance Temperature: 17.7 - 20.2 C Total suspended so lid s and pH on day o f testing: 4.1 g/L (TSS in final: 1.6 g/L), initial pH 7.7 and final pH 7.1.
002476
Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Values corrected to 20C for calculations.
R E S U L T S _____________________________________________ Nominal concentrations: Blank control, and 1000 mg/L, in duplicate.
Reference substance at 10 mg/L.
Element values: 30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L
Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS_______________________________________________ The FC-603F 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L, the highest concentration tested. Mean value of 10.1 % inhibition at this concentration. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________ _______________ R eliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution..
REFERENCES________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M 1655,1994.
OTHER______________________________________________________
Last changed: 6/26/00
002477
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED UQUOR
U=l
Date
Analyst
Page t
of M
Type Test
ACTIVATED SLUDGE MIXED LIQUOR
Date Obtained 'T - \Q ~^va
Date Used o~- v C ^ S H
Mixed Liquor Suspended Solids fM LS S I u\ v\ g /L Adjusted to _________ g/L
MLSS in final test solution
Vv Cc g/L
Initial pH
(1st test soin, prepared)
Final pH ~~t V (last soin, prepared)
Com m ents ____________________________
ISSTSUBSTANCS
Sample Description Initial pH of highest cone, tested t *cc.c
U
Com m ents __________________________________
"l^
Adjusted to pH
REFERENCE SUBSTANCE Sam ple Description 3 >5 - ~> 3 V \ V a c Initial pH of stock solution ( SCC* mg/L) -
\ >`5 '
Adjusted to pH
00247S
BEST COPY AVAILABLE
-
ENVIRONMENTAL LABORATORY
LR rf\ 1(r. i>?>
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION
Test Substance: ^ " C
Sample Description:
Analyst: Q>
________
Date:
(V -cA Sj 1^- ^
_______________________________ . ---------------------------------- :---------------
Test Cone. mg added
mq/L
oer 500 mL
Cca' s^ V\ --
*GOC-
C>
m LM Q (1) water per 500 mL
mL syn. sewage
mL microbial
feed w r SCKLmL irwcvlvm trer 500 mL
Total Volume -------- mL------- .
____ _______
ifC C ;
____ 12_____
"AC'C
____ - J/---/S , !___
~3TfN>\^Cv.\ ~>NV--V-vcVtrA ccoc'.
; ';
'?>.% ' ' ," vif' . *t V;
~~ r.
' .....'
;; : v:':-
C `W<
b e s t c o p y a v a il a b l e
ENVIRONMENTAL LABORATORY
I^ ^ l (pfj
lr
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: T -L . f c c r j F f L c A ^ TCA>S______________________________________
Source of Activated Sludge Mixed Liquor: .v\W - ' . v . P v c cA . .n v-o^. v . ct-,a ,
Date Received: * r - ^
Date Used: *> ~ l O ' ^ M
Analyst: 5 V S i / TL -T ^
30-MINUTE EXPOSURE
Test Substance Concentration (TM /0 0 .5
1 .0
DISSOLVED OXYGEN CONCENTRATIONS, mgrt.
TIME- MINUT ES 1.5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0
Temp 5 .5 C
BKCONTROL 1 >cr:n
(b>v \
* 1 S'-"I
6 . 1 H .l
d .'l a. 6
M .3 H .O \ fe 1 . 3
3-<o 3.^' c .q o .f;
--
1 .30. <
Tit s . s 6 -0 M .~l u .3 3 A 3 - 5 3- 1 5 . 5 ' 3 . \
BKCONTROL II <4S
" in <>
I.M
1 - V Co.'6 G.6T Q>. x S \ ct
S' *. W i 1 6 .3
s.c.
1 1. ~i
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS. mg/L
Test Substance Concentration ( " I l ) 0 .5
[ BKCONTROL 1 .V
v i O Q ( V -
. , 1 - H ^
"Tit ro>
' - \ k(o
iy:|&K*-5V ''.riVT <'1
BK CONTROL
J
T IM E -M IN I!TES
Temp
1.0 1.5 j 2 .0 2 .5 3 .0 .3 .5 4 .0 4 .5 5 .0 5 .5
r- m .
*t .;%
C
j ' .
".V Co.% (o.Cc <.3
'SiOA
hA
1.3 /S .S
& .0
S . 3 S'. O
'A.O
a . c\
"i.3 1 . V G *tV & . 5
VfV^-
3 , ! mm. mm m m * 1
US ISI
m CMS i s f n - AA'fT mm
<-l.p /< i.o
:iW 8 l
mm m m
iR M l - m m 'jmuai>
ORm^WW*TA`I SBI[flJIi .A.... .. .
X. i
p H o f . activated sludge mixed
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Substance: FC 603F (Lot #501)
Analyst: JBD
^ Date:; j W M
vfit
sm
' 'Ji-'- '
i. i -$} " I
30-Mlnute Exposure mg/L 0.5 1.0 1.5
<v.- - K BK1 6.4 6.1 5.7
1000.0 4.1 3 .5 3.1 Dup. 5.9 5.4 5.0
Dissolved Oxygen Concentrations, mg/L
2.0 2.5 3.0
* ..
i,
3.5 4.0 ///
4.5 5.0 , /<
5.5 Tamp, *C i&K
R (D
Ra (2 )` % Inh. (3)
5.4 5.1 4.7 4.3 4.0 3.6 3.2 2.9
20.1
0.7152 0.7102 is&tel&F
~, .
2 .7 ' 2.3 2.0 1.6 1.3 0.9 0.5 4.7 4.3 3.9 3.5 3.1 2.8 2.5 2.1
20.2 19.8
0 .7 3 6 7 0 .7 2 6 5 - > v 0 -: ` 0.7358 0.7461 O '? - '-
3 BK2
8.0 7.7 7.4 7.1 6 .8 6.5 6.1 5 .9 5 .6 5 .3 5 .0
% diff in controls (4): 0 .7
EC50 (95% Cl): ? .10W.mfl/L
17.7
0.6012 0.7055
F aCm *is
(5):
-
0 .7 0 7 8 .a;-->;.
k. .
3-Hour Exposure
Dissolved Oxygen Concentrations, mg/l
m g/L 0.5 1.0 1.5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 Temp,*C ` R (D Ra (2) % Inh. (3)
-
fS ^ H *
11111
tg |ii
mmm
BK1 7.4 7.1 6.8 6 .6 6 .3 5.8 5 .6 5 .3 4 .9 4 .7 4 .3
18.8
0.6255 0.6799
,y r~
ifkV-v>` ` ' iK'
1000.0 6.4 6.0 5.7 5 .3 5 .0 4.7 4.4 4 .0 3.7 3.4 3 .0 Dup. 7 .6 7.3 7.1 6 .8 6.5 6.3 6.0 5 .7 5.5 5 .2 4 .8
BK2 7.9 7.5 7 .3 6.9 6 .6 6.3 5.9 5 .6 5.3 5 .0 4 .6
% diff in controls (4): 7.3
EC 50 (95% Cl): >-1000. mo/L
Method: N/A
19.5 19.0
18.3
0.6594 0.5455
0.6827 -3 .2
0.5847 gffj7 ' *At:i
'
.'.V-Viv
0 .6 4 9 7 0 .7 3 1 2 wTli-` *
RaCm,(5)^LP.7Q55
*5 ;# ^
(t) R - Oxygen uptake rata (mg 02/l-m ln) (2) Ra R adjusted to 20*C (R/e.Ea) (3) % Inhibition. |(RaCm)-RaS/(RaCm)|100 (4) % dWerance in control: IIRaSk1-Ra8k2l/RaCm]100 (Two control* must ba within 15% of each other.)
0 1.072
RaCm- mean adjusted R. control*
Bk blank control. S> ample
Tob sampta tamp
,, ^ ,.
(S) Mean adjustad R, oontrol- [RaBkt *R*Bk2y2
x Toba-20*C
*V- s
'*3r*-iiti*<i**v*
BEST COPY AVAILABLE
ENVIRNOMENTAL LABORATORY
Ie\ 4
iYU(c5'6',
LR m \M Q ~
OECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION
Test Substance :
- \>.cWV.C-'.s 4 \'e \
Sample Description: f
Analyst: CS
_________
^ V _(v\-\
CV-cY * O ^vO ^C O ^
Date:
Stock Solution
Preparation:
c*. -'-C-
1* T p o, \ L v-r V k w ^ n c
^ vC^ s, ^vV~- Kcv - \ ~ CVa--ck" vv- a_.sV,-..wytu ~*^o ^^'-rW^C-XX-T*. Tes t_v\pfr^C:S . T^C.,V\V A^XvW^ ~C\
MC -a w ul. . \ 1 ` ' i .. . i
...
L K .. V
. ' Uv
^ y . ' . ^ L .viiC'^fcQ^:
y vc '-T-.V\ivVv crS__________________ _______________ ^ ____ '-v
s&feulfc- orvvU'- c>. \c
^ <x \ npcA vcVti^C. A \ L ,
v ^ - t/t-\ .,V c ^ l:A<~
"l.i
.Vcfrj W
Test Cone.
m al__
mL added mL MQ(1) water
per 500 mL
por 5QQ mL
mL syn. sewage feed per 5QQ mL
J351L
SO. Jl-
vCp --Xq-
mL microbial inoculum per 500 mL
Total Volume mL
fo O C l r ijc jy
Q.O-- -S Q Q : .
BEST COPY AVAILABLE
E N VIR O N M EN TA L LA B O R A TO R Y
_R rrm iG - K 3
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST 0 9
RAW DATA
Test S u b stan ce: cS, ~~ ~ Q ^ c V \\g A C ^ ; > W o o \ ___________________ __ ______________
Source of Activated Sludge Mixed Liquor:
Date Received: S' - vC. - c\
lO ir -v Rv
mAj
Date Used: S' - .G- ci H
Analyst:
j
30-MINUTE EXPOSURE
Test Substance Concentration ( /l ) 0 .5
BKCONTROL I
t.M 1 .S
DISSOLVED OXYGEN CONCENTRATIONS. rrxyL
TIME- MINUT ES 1.0 1 .5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0
(p* \
5 '.T
R -O
<b.?,
3". 1
<b-G T
H ,T HS LI . O (r>.-i G V 5 Vs
3 Sr 50
3 -* 5 .H
Temp 5 .5 PC
x, f'i
i
IP .3
BKCONTROL II <5J nSUiOU n.T -* i 1 * \ <bS 6 . 0 <b. t
S `.G -5 V T 5 .3
-0
t *K 7
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mgrt.
Test Substance . Concentration (s/ l ) 0 .5
BKCONTROL 1
7` H
;j" .. " V*','I ; :'V- . ' "* ' ,\v -
1.0 1.5
T . 1 fc.S ? .o 7 3
; mm
2 .0
.fc
<|A t
m ,?
T IM E -MINUTES
3 .0
3 .5 ; * .-i.1.
Temp
4M ,5 .0 v 5.5:,
'V v f.v .-
63*
<s-.
-'.V'T'-' "
-
88.d r S r f / . T H i3
T I'
T . O C-jS - ^ ; f - v ...V; . >.. . -'.- .t4 m :r-t
",.' .,
t .r
*m m m S'V: ;.:{
m m . m m i
mm
H 5F
RflSsssWs- m s m
T S f' i m i-i-'-.' V"*jfcsfSisAtie
BigriaieiH
g p ^ f ^ t h ^ t ^ s l u i c f q e ;rmxedT Hauo'f: % tiaP-- ^P ^P ii/iighesrccincentration.tested: .initial
PP
tSajtK*,!?
m
spin al*____ _________
lFin^m7.(mm$
002483
M lliilitittfiN itil
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Substance: 3.5 Dichlorophenol Reference Toxicant
Analyst: JBD/RJW
30-Mlnute Exposure
mg/L 0.5
1.0 1.5 2.0
'vj: s
BK1 6.4 6.1 5.7 5.4
2.5 ;
3.0
3.5 v'' '
4 .<0*'**&.
4.5
5.0 W ?M .
5.5 f.
5.1 4.7 4.3 4.0 3.6 3.2 2.9
10.0 7.5 7.3 7.0 6.8 6.5 6.3 6.1 5.8 5.6 5.4 5.2
t
* _ v-^'LR:^M16^51
,..... . ,, V&-0
Temp, *C R *(1 )^ Ra(2)? %r lnbf(3)$ \
*** ' s'
mmmmm i
20.1
0 .7 1 5 2 0 .7 1 0 2 im x ;& i
19.3
0.4733
0.4969 SW3038* i
.Vi
:
BEST COPY AVAILABLE
BK2 8.0 7.7 7.4 7.1 6.8 6.5 6.1 5.9 5.6 5.3 5.0
% diff in controls (4): 0.7
17.7
0.6012 0.7055 FlaCm (5):,%0.7078v?i
3-Hour Exposure
Dissolved Oxygen Concentrations, mg/l
' ... V
m g/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5 .0 5.5 Temp,*C | R (1) Ra (2) % Inh. (3)
Vs 'Cv'V
' *? >* ' j f t s t f a
BK1 7.4 7.1 6.8 6.6 6.3 5.8 5.6 5.3 4.9 4 .7 4.3
18.8 0.6255 0.6799
1
10.0 8.2 8.0 7.8 7.6 7.4 7.1 7.0 6.8 6.7 6.5 6.3
19.7
0 .3 7 3 3 0 .3 8 1 2 m ; 4 6 f& # I
BK2 7.9 7.5 7.3 6.9 6.6 6.3 5.9 5.6 5.3 5 .0 4 .6
% diff in controls (4): 7.3
18.3
- '
tm m m . 1
m m m w? i
M O T!
0.6497 0.7312
RaCm (5):M O:-7055j
(1) R* Oxygen uptake rale (mg 02/l-m in) (2) Ra* R adjusted to 20*C> [fVo E i] (3) % InNWtkJO. ((RaCm)-RaSl(RaCm)|100 (4) % dflerence In eontmls: |IRaBk1-RaBk2l/RaCm)100 (Two controlt muat be within 15% ot each other.) (5) Mean ad|u*ted R. com m it- (R a8*nR a8k2y2
e * 1.072 RaCm* mean adjusted R. controls |Bk> blank oontral. S * sample Tobe* sample lemp Tobs-20*C
Chain of Custody Record
3M Environmental Laboratory
F*m3S*-PWO
A dam s P r < * / Vi e
C t|> . S > m . 7 p C o a t
3M P ro ject Tip# 3M f c * ty C ontact 3M ftto p h o n t N ta n O ff.
01934
**5^/ f. o .
. v,;
i~
9MC
BEST COPY AVAILABLE N aO H VOC gV', t. T o ta l C o n ta in e rs o t E a c h Ite m ? .. -. -t
S *rW G Br (Port)
*- c L - i ^
S a m p rk n ilu d
J
bs<r$ o
T___
j
Item
\ / Sample NOescription
**1 W1 L * ? v / A cM
2
3
4
5
Com p
O ct*
s'
" s y /o /? jf
Date Time Matrix
Number Containers of each
P T M W V S tlV M
ft
J8 Xr
f T"
{
6 7 8 9 10 11 12 13
Item # /
Relinqu/ted By/Affiliation
Time
Date
Shipped Via
r z / VV ^ - 5 7 c ^
Onp*** Accompanying Samples
UM Pape O'Qnator
G Samples Split S e(B ' tt^hm erit|||^^^
v ^ i 15-
1
a * * * * * * * * t o
.M1; f .' -
* i >>.-^. *'-iV^ . '
$8 . ' ir'**>.<?'. U.f
-" v " '* 4 P S lf! f ;*e
-.---
-^
* -V
Received By/Afflliation Z ^ T im e fP ft a t e i
3 A / " fW i0 ^
s-rm m
ft
'iSUjaBS
. . v ' -*y
See Reverse Side For Instructions!
