Document 5LkkayEB4ZDmjYd323R2jx1rV
_ 075S
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE_______________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 519. The test sample is FC203CE (2/21/89). Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. Antifbamer SWS 214 was added at a nominal concentration of 200 mg/L.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Date may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD _________________________ ________________
M ethod: OECD 209 GLP: No Y ear Com pleted: 1990 A nalytical m onitoring: Dissolved oxygen concentrations, pH and temperature. S tatistical m ethods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro W aste Treatment Plant, St. Paul, MN T est condition:
Dilution w ater: Millipore Milli-QTM water Synthetic sew age: per OECD method Reference and te st solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2SO4 and diluted to 1L with Milli-QTM water. The pH of the reference substance was measured to be 7.4. Test substance was added directly to the test vessels. Antifoamer SWS 214 was added to the test vessels at 200 mg/L from a stock solution prepared by diluting 2000 mg of SW S 214 in 500 mL Milli-QTM water. The test substance and antifoamer appeared miscible in water. Test vessels: Not given Num ber o f concentrations: three plus 1 reference substance and 2 blank controls Tem perature: 20 - 22 C
00113
Total suspended solids and pH on day o f testing: 5.033 g/L and initial pH 6.6 and final pH 6.9, Initial TSS of 1000 mg/L concentration was 2.027 g/L, pH 6.6 while final TSS of 1000 mg/L concentration was 2.213 g/L, pH 6.8. Elem ent Basis: Respiration inhibition as determined by oxygen consumption.
Rem arks: Values corrected to 20 C for calculations.
RESULTS_____________________________________________________ ___
Nom inal concentrations: 2 Blank controls, reference substance, 320, 560 and 1000 mg/L
Elem ent values: 30-minute EC50* >1000 mg/L 3-hour EC50= >1000 mg/L
Element values based on nominal concentrations.
Rem arks: There was a slight reduction in the amount of foam formed in the 1000 mg/L exposure concentration when compared to FC-203CE without antifoamer.
Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S ___________________________________________________
The test substance 3-hour EC50for activated sludge respiration inhibition was determined to be >1000 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES__________________ _________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number H148 0-3 ,19 90.
001133
OTHER Last changed: 6/26/00
001134
Type Teat
7
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
1r y U 4 f t Q - s ' D a te ^ jq ^ O
Analyst^ g '^ S . ~
\nVnK-Hnrt 'list- HfTPl #ZCf(
Source Date Obtained
} JSLTT^uii, NlkL ____
^,J\Q j _____________Date Used
jjQCS
Mixed Liquor Suspended Solids (MLSS)
g/L Adjustment
pH L>,U>j -Pinal L>.9 Comments
zLL
Test Substance
Sample D e scrip tio n P C IO X C E . LnfcftQ ( 2j2if& i) y ^ A t t & f e a m c r -S W S 1*4 /200maiO
kicj'vcst CoMCekfaratlovt-trtjed'lb o o w\^lu
^
SamPle PH-mUial 1 * .^ lo.ft Adjustment_____________________________________
co m m e n tsM L ^ o f IQOQy t q /l t e s t s d n .j ivtfUai LiOEMflib,
Reference Substance
Sample Description X. S-rTsirWilrivvirih^irkl H ip e s t c o * c & b i K -----
SamPle PH.mttiflA U .T , ,-firuU toiB________ Adjustment.
Connents
^nlu.. tp U ^ 4
Exposure Conditions
Test Duration ^ H g U T S . ..
Test Temperature l Q 2
C
Comments_______________________
001135
orm 10630 6 0 PW O
Lab Bequest No. f c t U A f i l2 L
iampleDescription
Environm ental Lab -- W ork Sheet
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
-----------------------Test Snlntinns Preparation___
Page
t
of ^ 3 ____
Date: __/ / * ? f O
Analyst:
7
?i>4- h r ^ uw^Il .V UMiY/MugO w p ^ k K i .
s w s z.\4
.a h . z o o
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5 ft w t l M i l l Lrfy. a c H 1K pLj = H n n g . ( S l $ 2.V^..ug ^ m ^ cV?\rj S r b d c b M .
-- E o ^ -Z -Q Q m a J U i - .1.00 .m d / s m L ? ....U s e d 5 v K i3 ^ L ^ Q jrv4iu.t______________
est Cone, mg aefaed mlMQ water ml syn. sewage ml microbial
Total volume
5 2 .0 ________LLcO___ +. 2~sq -h Z fs 4- U f ________ ____ r 2 o O __________ =___> 2 0 0 ___
5 io Q _______ Z p fo +- 2.5<1 4 fN f U f ________t ____ c S n n ___________ S Q f l_______
Q Q * _____ t5 0 -Q .... +___ zs<=\ 4- 2Js * \u >
___ c 2 __________ - = . ,, . iSD .Q ___
^ C b.^r p l ] I ___ O ____ _^QMr v H +~ \L?
-v-____3LOD____________ ^ - S Q O ___
S k lA ir f d f i k , CovtAvV X \n)v-Wy,, I S ^ U V ^ Q - l . ___________________________ _____________
r - ^ d u o ^ w \ v l awt^LV^fc f fo A w L .-fb v v v ig rl iP V y p v i. r a w jp a m i -- tan f r C & D ^ C iL w ^ U n u d c ajA K -ftavU <ir.___________________________________ 7 K . 5 - j g ^ L s r^ lu B m VAa&s r t u f t i c a t o i U& a i Wv M e a s u r iv ^ T T S S w -------------
001136
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIO N TEST
RAW DATA
#209
LR-
T e st S ubstance: FfC -Z Q Z C & L f o t ^ / 9 ( z j n j 9 f t )
/irttJ fO c ltn rr< S W > 5 Z J ^ J .
Source o f A c tiv a te d Sludge Mixed L iq u o r : / - f o m Ia /Z<4-r - T y ~ r T frs frT f, S + 'f h u /. A ^ t
Date R e ce ive d :,
J tfD _________
Date Used: 5 / / q / 9 n __________
A n a lyst-
30 - MINUTE EXPOSURE
le s t substance
" 7 I S P MINUTE
?C o n c e n tra tio n (m g/L) 0 .5 1 .0 1 .5 2.0 2 .5 3 .0 3.5fc 4 .0 4 .5 5 .0 5 .5
CONTROL I
^7D ftlaO -
Y r/ 55 5 ,1
{<>.*)
AA 2.2
A ? 5 , V ,5,0 H ,b
on z lk s
\ XZ,
212S' / . In 1 ,0 / f f c s
H-<K A
7
2 ,5 7 /
20 20
10 O
& 4 z > r\ ?sO 1 , 5 0 . 5
O
CONTROL I I
M . tL L . 2J. & ,
i^ lV
Jute- Z A
3 - HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS mo/L
le s t Substance
TIME-M INUTES
' tem
cC o n c e n tra tio n (m g/L) 0 .5 1 .0 1 .5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 .5 .5
CONTROL I
15 9 A i i ? ,5,2
5,7V , 5 V , /
5 , 2 A 8 ? u 4 2 , 0 ?>\
A ipD
1o m
,5 0 5 ,2 to, 5
Y ,5 5 ,7 5 ,9
^ 0 3 5 , / M iS 5 ,9 5 , 5
5 io 5 , 5 ,5 ,0
2 ,fi 2 ,5 5 7 2 .7
*4.(n * / , 5
/,<? / ,, 5 I<Z<
2 .5 A 9 /,,5
?\.ln 5 , 5
0 .9 /,/ 2 ,,9
Zi 71
2/
CONTROL I I
5 . 9 5 . 5 5 / ^,U> H-,2. A R
pH o f A c tiv a te d S ludge M ixed L iq u o r : pH o f h ig h e s t c o n c e n tr a tio n te s te d : Test Tem perature: JL O -Z Z C ,
I n i t i a l (^,(o I n i t i a l i fl i 0
,5 ^ 2 . S
L(x> 2 2
F in a l (p.Cj
F inal
U ,r>
001137
Test Substance
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST *209 CALCULATION OF RESULTS
Date Analyst
L t & /9 (z J ^ 9 o ) w iiA A H h 'f /T lfr'S W Z .I^ iZ o n rn ^ fl^ f1
30 - MINUTE EXPOSURE
Test Substance Concentration (mc/L) RSBinHBBHHi
-.....
............ m o o
Oxygen Uptake Rate R
O.ftMqi i.n*^
... l.
R Connected^ to 20 C
n.fMlL? M141
uqnio
U ifo o n .
% Inhibition in f7. Respiration Rate^'
---
-41
<?o&
V-
o a m o u i___________
Q.ft2^o______
Percent Difference in Controls^
2 fin
ECjg (95% Confidence Limits) mg/L__________
3 - u n IB EXPOSURE
Test Substance Concentration (mg/L)
Oxygen Uptake Rate R
W S *2 0 .SI//) in n o
_
/ 3 * \ o _ n .fto ift
o ,fio n o 0 ,( / ^ 5
CONTROL II
________S h d n D l ______________
___ L _
R Corrected^ to 20*C
% Inhibition in Respiration Rate^ZJ
n .n io n s . _ rv 7(^4 1
on 1 r io 1ft
.r . / ..........- ......................... /'
o . n f t s ? , _____ ;______ 1 ______________ _
- Oxygen uptake rate (mg 0,/L-min.) ',obs Sample temp.,*C c
k- 1.047, x - T,obs - 20*C : Control
001138
Type Test.&4-W AfcM
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
D a te .a to /^ Q Analys
IvlVubrbiAYl ^T e st (O F
Source MfWn
-K ajA -k,
j M jA ------------------------------
Date Obtained
D ______________Date Used
52 2 *) ___________
Mixed L iquor Suspended S o lid s (MLSS)
g /L Adjustment
g /L
pH. iiyW .It.1!, -final Comments
________________ .
-
..
b e s t COPY AVAILABLE
Test Substance
Sample Description ^ ^ g ^ - ^ foaiivv Vvi^nc&t' coviccirt^stioA. -tfis ta ij vooovri^lu 1
Adria^H'.
5*
Sample pHJ^-jM
____ Adjustment_______________________
Comments
.aftturKr^ ifrU lr>n.______________ ,_________________
Reference Substance
Sample D e s c rip tio n , g ,
_______
Wa^est- cowceifchrei4oA-+cs^itij 10
Sample pE T ^ a l l.<* ^ - f W l *i.fl______ Adjustment
Comments,^ ^ ^
.
Exposure Conditions
Test Duration
^nuir-s
T est Temperature_...l B r - i a Comments ___
oC
001139
Form 1Q820-8 0 - PW O
Lab Request No. U \ 4 f i Q
Sample Description
Environm ental Lab -- W ork Sheet
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
Page
1
Date:
Analyst:
of ..
Stock and Test Solutions Preparation
5W S 14 A rri-i-tK g m er (m n /ftla )
^ A d ria n ^
STOCK SOLUTION: ZQQQ yY1q /S O Q yY \Ij ___ K ^ CH, l m l o -=LMrtrtg..
-=-JZD Q O
...^ V w (5 \ 4 / r s o o Vu L j- H l 11
-- 2 J 4 u p A j rrv s P jb lrJ V
Ui ahOTT_____
.^ U l*?1
Test Cone. ml stock soin mlMQ water ml syn. sewage ml microbial per 500 ml___ per 500 ml fe e c ^ j3 e r^ 0 0 jm l_ in o m
Total volume ___ ml
i_______ n s _____ ___ isq + >u
____ 2on________ = son
^HrfrTc1 T P V j
w U V l \ 3 ^ V4 H B O -rL s H -l.
001140
ENVIRONMENTAL LABORATORY
lr J i l i A f i O r S _____ page. 2 . 0 ^ 3
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test S u b s t a n c e : 214 A
n f (y lh Iflb ) S \\iflb v \g & C W -pflrrttiQ vtj A x ir la ^ V t) .
Source o f A c tiv a te d Sludge M ixed L io u o r:
0
Date R e ceive di .'& J i.& fa o __________
Date Used:
____________
AnalysfT ~
30 - MINUTE EXPOSURE
le s t Substance
T T MINUTES
C oncentration (mg/L) 0.5 1 .0 1 .5 2 .0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 ?
CONTROL I in a n
*> .0
U fi 1 ,4
,CS,^ 4 . 1 H. 2 , 7 *n ih /
l . f t O .fS
?S,1
.il 1.Y7 I R \8
CONTROL I I
-S A .
JbLCL.
-
scy a . iu s . J L 3 - J L ik D - S a x
3 - HOUR EXPOSURE
le s t Substance C oncentration (mg/L)
CONTROL I
0.5 1 .0 ( ,, ,5 I .l *>.n 2 ,2
DISSOLVED OXYGEN CONCENTRATIONS mo/L
Tim e - mINUTES 1 .5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
4 .R 4 .H -,
1.5 T | . 0 z p /
iS ,5L .,4 2 ..0 \,S
TEP 5 .5 C
1 .0 ts
fl
CONTROL I I
5 .8
4 4 3 .
z .n z .,4 l . l
pH o f A c tiv a te d S ludg e M fxed L iq u o r :
pH o f h ig h e s t c o n c e n tr a tio n t e s t e d : Test Tem perature: 1 8 " 1 ^ ^
I n it ia l (p .l In itia l
F in a l (>.9
F i n a lio. 8
o.s I 001141
ENVIRONMENTAL LABORATORY
lr U m g n - *
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Pate ]z * > iQ n Analyst *,
Test aihttanrA^W<> Atfc^bflKrv>r (q / M / f l l n ) S T L A d v n l ^ t ^ ^
30 - MINUTE EXPOSURE
Test Substance Concentration (mg/L)
L I ________
1000
Oxygen Uptake Rate R
l.0TL*4
. I.OR4q.....
R Corrected^ to 20*C
1.|ftO
LIm b
% Inhibition in Respiration Rate*-2'
L>
!LJI___________
tPercent Difference in Controls^ ECS0 (951 Confidence Limits) mg/L,
4.B < %
Test Substance Concentration (mg/L)
Oxygen Uptake Rate R
inon
LlSSb
\ . t & p n _____
R Corrected to 20C
1.l u t i l i mo
% Inhibition in ,2i Respiration Rate^J
-
CONTROL II
1.0' i v i
Percent Difference in Controls(3)
EC^ (951 Confidence Limits) mg/L.
___ l.'lfSOp___;___
(1) Temp. Corrections --
(mg o2/L-min.)
(3) I Difference
X 10
/RC, + RC^\
(2)
I
In
h
ib
itio
\ n*
*
-1;-2----7L-
-
JRUg
x
ioo
K RCj
Where:
R Oxygen uptake rate (mg 0,/L-min.)
T,obs Sample temp./C L ft - 1.047, x - T,obs - 20*C C " Control
The two control respiration rates should be within 15% of each other
001.142
