Document 5LbaBZJ2620X3zBozxJN4mp2V
Vista Chemicol Company
Lake Charles Chemical Plant Old Spanish Trail, P.O. Box 727
CERTIFIED--RETURN RECEIPT REQUESTED #P241 411 248
Westlake, Louisiana 70669 Phone (318) 494-5140
June 25/ 1990
Ms. Evelyn Rosoborough (6W-PS) Permit Branch Water Management Division EPA Region 6 1445 Ross Avenue Dallas, Texas 75202-2733
Re:
Section 308 Letter of June 9, 1989 Requiring Chronic Biotoxicity Testing NPDES Permit No. LA000336
VISTA
Dear Ms. Rosoborough:
Please find enclosed the summary reports, Table I data, and the formal reports for the monthly sampling for May initiated May 14, 1990.
If you have any questions concerning this data, please contact Paul Fetzer at (318)494-5067.
Sincerely,
M. G. Hayes Director of Environmental Control Lake Charles Chemical Complex
CWH 000010099
PERMITTEE: PERMIT NO: OUTFALL NO:
Vista Chemical LA000336 EPAOOl
ENCLOSURE 1 (PAGE 5 OF 11)
TABLE 1 BIOMONITORING REPORTING CERIODAPHNIA DUBIA SURVIVAL AND REPRODUCTION
Date composite collected:
#1: FROM 0700 5-13-90________ TO
#2: FROM 0700 5-15-90
TO
#3: FROM 0700 5-17-90________ TO
0700 5-14-90
0700 5-16-90 0700 5-18-90
Date test initiated: 5-14-90____________________
Dilution water used: Receiving water
X Reconstituted water
NUMBER OF YOUNG PRODUCED PER.FEMALE G> 7 DAYS Percent effluent (%)
REP 0% 52% 59% 67% 76% 86% 13% 26%
21 24
7 25 20
9 19 19
23 17 184 26
cui-i oooo.toiot
PERMITTEE: Vista Chemical PERMIT NO: LA000336 OUTFALL NO: EPAOOl
ENCLOSURE 1 (PAGE 6 OF 11)
TABLE 1
CERIODAPHNIA OUBIA SURVIVAL AND REPRODUCTION TEST
1. Dunnett's Procedure or Steel's Many-One Rank Test as appropriate:
Is the mean number of young produced per female significantly less (p=0.05) than the control's mean number of young female for the % effluent corresponding to (significant non-lethal effects):
a.) LOW FLOW OR CRITICAL DILUTION ( 76 %):
X yES
_______ NO
PERCENT SURVIVAL
Percent Effluent
, Time of Reading
24 h
0% 52% 100 100
59% 67% 76% 86%
13% 26%
100 40 50 20 100 100
48h 7 day
90 100 90 40
90 30 50 10 30 0 0 0
90 100 90 90
CV%*
* coefficient of variation= standard deviation X 100/mean
2. Fisher's Exact Test:
Is the mean survival at 7 days significantly less (p=0.05) than the mean control survival for the % effluent corresponding to (lethality):
a.) LOW FLOW OR CRITICAL DILUTION ( 76 %):
____x__ YES ______ NO
3. Enter percent effluent corresponding to each NOEL below and circle lowest
number:
a.) NOEL survival = 26 % effluent
b.) NOEL reproduction =
13 . % effluent
4. If you answered NO to l.a) and 2.a) enter [P] otherwise enter
[F]
CWH OOOO10102
PERMITTEE:
PERMIT NO: OUTFALL NO:
Vista Chemical
LA333006 EPAOOl
ENCLOSURE I (PAGE 7 OF 11)
TABLE 1
BIOMONITORING REPORTING
FATHEAD MINNOW LARVAE GROWTH AND SURVIVAL (Pimephales promelas)
Date composite collected:
#1: FROM #2: FROM #3: FROM
0700 0700 0700
5-13-90 5-15-90 5-17-90
Date test initiated:
5-14-90
TO 0700 5-14-90 TO 0700 5-16-90
TO 0700 5-18-90_____
Dilution water used: Receiving water
X Reconstituted water
DATA TABLE FOR GROWTH OF FATHEAD MINNOWS
Effluent Cone (%)
Average Dry Weight
in milligrams in
replicate chambers
AB
C
D
MEAN DRY WEIGHT
(mg)
CV%*
0% 52% 59% 67% 76% 86% 13% 26%
0.34 0.44 0.36
0.15 0.20 0.30
0.25 0.13 0.20
0.13 0.13 0.20
0.05 0.23 0,10
0.10 ____ uTzrr U. Zb 0.36 0.41
0.20
03 0,26
0.39
0.38
0.33
0.24
0.10
0.17
0.20
0.17
0.05
0.11
0.10 "0.41"
0.47
0.13 "08
0.37
10
29
34
20
69
35 "2D
21
* coefficient of variation = standard deviation x 100/mean
1. Dunnett's Procedure:
Is the mean dry weight (growth) at 7 days.effluent significantly less (p=0.05) than the control's mean dry weight (growth) for the % effluent corresponding to (significant non-lethal effects):
a.) LOW FLOW OR CRITICAL DILUTION (76%):
___ X_ YES ____ _ NO
CWH 000010103
PERMITTEE: PERMIT NO: OUTFALL NO:
Vista Chemical LA000336 EPAOOl
ENCLOSURE 1 (PAGE 8 OF 11)
TABLE 1
BIOMONITORING REPORTING FATHEAD MINNOW GROWTH AND SURVIVAL TEST
DATA TABLE FOR FATHEAD MINNOWS SURVIVAL
Effluent Cone (%)
Percent Survival in Replicate Chambers AB C D
MEAN PERCENT SURVIVAL
24h 48h 7 days CV%*
0%
100 90 100 100
100 100
98
52%
40 20 40 40
100 100
35
59%
20 30 30 10
100 100
23
67% -
30 30 50 30 " 100 100
35
76%
20 30 13 20
100 100
21
86%
--m
26%
20 0 10 10 100 100
/O 80-- 80 90 "TOO 1U0
80 80 70 60
100 100
10 80 73
* coefficient of variation = standard deviation x 100/mean
4
25
37
25
35
71 9
11
2. Dunnett's Procedure or Steel's Many-One Rank Test as appropriate:
Is the mean survival at 7 days significantly less (p=0.05) than the mean control survival for the % effluent corresponding to:
a.) LOW FLOW OR CRITICAL DILUTION (76%):
____>L YES ______ NO
3. Enter percent effluent corresponding to each NOEL below and circle lowest
number:
a.) NOEL survival = < 13
% effluent
b.) NOEL reproduction =
NA*
% effluent
4. If you answered NO to l.a) and 2.a) enter [P] otherwise enter
rn: f
* The EPA recommends (EPA/600/4-89/001 Pg. 2 Section 12.3.1) that concentrati above NOEL be excluded from the hypothesis for growth test.
CUH 000010104