Document 5LMbRj5QV7LwknO3Ea9LqoGOz
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E. I, du Font de Nemours and Company
Haskell Laboratory for Toxicology and Industrial Medicine
AR226-2831
ACUTE ORAL 1EST
Procedure: The test material, as an aqueous suspension, was administered by intragastric intubation GMt-GD male rats in single doses, Fnrvivors were sacrificed 14 days later; the livers were removed analysis.
Results:
Suspension (%)
.
Dose ("'FAR?
Mortality*
Weight When Killed (e)
Body
Liver
L.W./B.W. X 100
Clinical Sig
25
17,000+
14 d.
332
17.50
5.3
lone
25
11,000+
14 d.
321
17.85
5.6
25
7,500+
14 d.
347
19.00
5.5
25
5,000
14 d.
346
17.40
5.0
25
3,400
14 d.
346
17.38
5.0
12
2,250
14 d.
320
15.20
4.8
L2
670
14 d.
349
17.00
4.9
* S - ( ) d. = Sacrificed ( ) days after dosing
t Administered in divided doses
TEN-DOSE ORAL SUBACUTE TEST
Procedure: The test material was six young adult ChR-CD male rats,
and were intubated with corn oil.
after the last dose.
administered by intragastric intubation, as a 15% aqueous suspensi five times a week for two weeks; in addition, six rats served as c
Three test and three control rats were sacrificed four hours and
Results:
Dose (mg/kg/dav)
Mortality
Weight of
Animals
After
10th
Tpse
Liver (g) Killed;
14 Days
After 10th Dose
L/W./B.W. X 100
Animals Killed; After 14 Days
10th
Dose
After
10th Dose
Gross Pathology^
on Liver
Animals Killed:
After 14 Days
10th
After
Dose 10th Dose
2200
(Control)
0/6
22.2
20.6
23.5
17.6
25.2
21.6
0/6
12.5
13.5
14.8
14.7
14.2
19.8
7.4
5.2
7.7
4.6
8.4
5 '
4.1
3.5
4.8
4.1
4.6
4.1
In addition, the following tissues were examined histologically: kidney, testis, epididy
trachea, brain, eyes, stomach, duodenum, thymus, spleen, bone marrow, pancreas, and adrenal; no com changes were noted in any of these organs.
* Gross and histopathologic code
No abnormality detected Large and heavy Reduction in number of centrilobular cytoplasmic vesicles
adm^nist&red orally in single doses, its Approximate LethaiDose (ALD) being greater than 17,000 mg/k body weight; single doses of 7500 mg/kg and above caused large livers 14 days after dosing.
Given at a repeated dose rate of 2200 mg/kg/day for a total of ten days over a two-week pe ir'ac test compound caused gross and gravimetric evidence of liver enlargement;histologically, Chere w reduction in the number of centrilobular cytoplasmic vesicles. These changes were apparently not re after the 14 day-recovery period.
Report by:
^UJ^Jl^' UJObljJ
Laurel S. Oral Toxicol
Approved by:
^// /c'i^ U Johri A./Ze
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