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AR226-3065 HASKXLL LABORATORY ?OR TOXICOLOGY AND XHDOSTRIAXi MZDICXNK DUPont HLR 905-96 STATIC. ACOTE 48-HOUR SCRKBHIMS TBST or H-21954 TO Daphnia aagn> PURPOSB: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL IOT'ORMA.TION HASKXLL HONBBR: 21954 TBST COOB:dfd& SPONSOR: DuPont NOTBBOOK XD: SOBKITTBR'S NOTEBOOK NO.: MATERIAL TBSTSa SYHOHySIS Specialty Chemicals COMPOSITION: casaKsszsssFES: OTHER CODES: CAS REGISTRY: PURITY; TBST COHDITIONS nominal concentrations tstd: 0, 0.5, 1.0, 50, 500, and 5000 mg/L. Page 1 of 2 Sonroany Sanffized. Does ncreconfain TSCA CBI DuPont HLR 905-96 HASKBLL LABORATORY FOR TOXICOLOOT AMD INDUSTRIAL MKDZCINX STATIC, ACUTB 48-HOUR SCRXKNINO TB3T OF H-21954 Paphnia naona. CONTXNUBD TEST CONDITIONS (Continuad) Diaaolvad Oxvgan and pH: Nominal Concaatrafcioa Kaplicata (9/L) At Test Initiation pB Diaablvad Oxygan (ag/L) Raplicata Raplicata A B A B At Tat pB Raplicata A B CoBplation Diaaolvad Qxygaa (g/L) Ranlicata A B 0 0.5 1.0 50 500 5000 8.8 8. 8 8.8 8. 8 8.8 8. 8 8.8 8. 8 8.7 8. 7 8.4 8. 4 8.6 8.6 8.4 8.4 8.8 8.6 8.6 8.4 8.4 8.8 8.6 8.6 8.4 8.4 8.8 8.6 8.6 8.4 8.4 8.8 8.6 8.5 8.3 8.3 S.8 8.5 8.6 8.3s 8.3^ 9.03 a Reading taken at approximately 24 hours after exposure, due to total immobility. 8.8 8.6 8.8 8.8 8.8 9.0a RKSULTS Based on visual observations, all test solutions were clear and colorless, except for the 5000 mg/L test solution which was cloudy, white in color. Ximnobilities were 0, 0, 0. 0, 60, and 100%, respectively. Ten percent of the daphnia in both the 50 and 500 rog/L test solutions were lethargic and at the bottom of the test vessel. The 48-hour BC50, based on nominal concentrations and immobility, was between 50 and 500 mg/L. All water quality parameters were within acceptable limits. CONCLUSIONS H-21954 exhibited slight toxicity in a 48-hour, unaerated, static acute test using Daphnia macma. < WORK BY, ^^ ^.W^ Jamds w. Wetzel(j Toxicology Associate /0//y/y^ Mo/Day/Year APPROVED BY: ^^^^^^ Michelle R. firown. B.A. Study Director ^'/'"//f^ Mo/Day/Year DISTRIBUTION ^^""-"o.oo^sCACe