Document 5LDqkJ06EKY0JQoJnXJOVBQqD

ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SUBSI"N E (SCUD) Identity:A mixturecontaininpgerfluorooctanesulfonwahtiec,h may alsobe referredto as PFOS, FC-95, oras a component of FC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-206, identifiebdy the laboratoryas "Sample C". Currentinformation indicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether, 78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylenemonooctylphenylether,and 0.05% benzotriazole. The following summary applies toa mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Methods forToxicityTests withAquaticOrganisms as formulatedby the Committee forToxicityTests withAquaticOrganisms Type: Acute static GLP: No Year completed: 1974 Species: Gammarus fasciatus Supplier:In-houseculturemsaintainedatBionomics,EG&G, Inc., Wareham, MA. Analyticalmonitoring: Not given. Exposure period: 48-hours Test organism age: 12 - 24 hours oldfourthinstar Statisticamlethod: TL50 (median tolerancelimitv)alues calculatedusinga linearregressionequation. Test conditions: Dilutionwater: Well water,aged 24 hours Dilutionwater chemistry: Not given Lighting: Not given Stock and testsolutionpreparation: Not given. Exposure vessels: 250 mL beakers with200 mL exposure solution Number of replicates:4 Number of organisms per replicate:5 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: Temperature range (0-48hours): 20 1 OC RESULTS NominalconcentrationBsl:ankcontro3l2,00,4200,5600,7500,and 10,000 mg/L. Element values: 48-hour TL5o = 5170 (4190 - 6370) mg/L 48-hour NOEL = 3200 mg/L Element valuesbased on nominalconcentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS- The FC-206 48-hourTL50 was determined tobe 5170 mg/L witha 95% confidenceintervaolf4190 to6370 mg/L. The 48-hour no observed effect level(NOEL) was 3200 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudy meets the criterifaorquality testing.However, the sample puritywas notproperlycharacterizedand the study lacksanalyticaclonfirmatioonftheamount offluorochemicalproportion inthe solution. REFERENCES Testwas conducted by Bionomics,EG&G, Inc.,ofWareham, MA atthe requestofthe 3M Company, St.Paul,MN, 1974. OTHER Last changed: 6/28/00. BIOASSAY REPORT SUBMITTED TO 3M COMPANY ST. PAUL, MINNESOTA ACUTE TOXICITY OF SAMPLE C TO WATER AND SCUD (Gammarus fasciatus). FLEA (Daphnia magna), Bionomics E G & G, Inc. Environmental Consultants 790 Main Street Wareham, Massachusetts August, 1974 METHODS AND MATERIALS These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G,, Inc., Wareham, Mass achusetts. The susceptibility of water flea (2@pn@ia magna), and scud (Gammarus fasciatus) to Sample C, a brown liquid, was evaluated under static bioassay conditions for a 48-hour period. Results were expressed as the median tolerance limit (TL the 50)' nominal concentration of the test compound in water causing 50 percent immobilization for the Da-phnia and Gammarus. The TL50 value and its 95% confidence intervals were calculated by converting the test conce ntrations and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. Procedures for static bioassays as described in the Methods for Toxicity Tests with Aquatic Organi-sms as formulated by the Coriimitteefor Toxicity Tests with Aquatic organisms were followed, except for those conditions described below. The 2ULnia, and Gammarus were acquired from laboratory cultures. The 0 20 C n@ia and Gammarus bioassays were conducted at + 1.0). -The invertebrate tests were conducted in Pag e two 250 ml beakers containing approximately 200 ml of well water which was aged for 24 hours. Five 12-24 hour old 2LELnia, and five.fourth instar Gammarus were placed in each beaker (4 replicates/concentration). Tn the Daphnia bioassays, Daphnia food 1 was added at'a rate of 0.5 ml/liter after the introduction of the compound into the test vessels. RESULTS The predicted TL 50 values- and 95% confidence intervals for the Daphnia- and Ggmmarus are presented in Table 1. Table 2 presents a summary of observed immobilization for each individual test after 24 and 48 hours. 1 Food was prepared by homogenizing 5 9 of starter trout food and 1 g of cerophyl in 100 ml of water. SUBMITTED BY: Bionomics E G & G, Inc. Environmental Consultants 790 Main Street Wareham, Massachusetts August, 1974 PREPARED BY: Robert E. Bentley -ol k- o7ix7t Chief, Acute Toxicit Investigations A PPROVED BY: Bevier Hasbrouck Sleight, III Director, Freshwater Programs Table 1 Acute toxicity of Sample C to Dap niaa and Gammarus.b The data are based on results of bioassays conducted at the fish toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species TL 50 -milligram active ingredient/liter 24 hour 48 hour No Effect Level (mg/1) Daphnia Gammarus. >10,000 5,850(4,810-7,110)0 3,200 5,310(4,340-6,510) 5,170(4,190-6,370) 3,200 a Bioassay conducted at 200C ( 1.0). Daphnia 12-24 hours.old at the initiation of tests. b Bioassays conducted at 200 c 1.0) Gammarus 12-24 hours old at the initiation of tests. c 95% confidence intervals. Table 2 Concentrations tested and observed percent immobilization for Daphnia and Gam-marus exposed to Sample C after 24 and 48 hours. Species Da-phnia Gammarus Concentration (mg/1) 10,000 7,5oo 5,6oo 4,200 3,200 control io,oob 7,500 5,600 4,200 -3,200 control immobilization observed 24 hour hour 20 100 25 50 0 4o 0 10 0 0 0 0 100 100 80 80 60 ?5 45 50 0 0 0 0