Document 5LD3bRXrN0Y7XraE9Zx6y3MKR

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M producfionlotnumber was notnoted.The testsample isFC-201AF. Currentinformatioinndicateistisa mixtureof 3% PFOS, 18.59% diethyleneglycolbutylether,37.81% water,14% ethyleneglycol, 8.25% Acrylicfoamer,9% sodium oetylsulfate0,.3% sodium laurylsulfate, 3% triethanolamine6,% sullfonateadlkylethersaltsa,nd 0.05% tolyltdazole. The followingsummary appliesto a mixture withincompletely characterizedconcentrationsof impuddes. Data may not accurately reflecthe toxicityof thetluorochemicalcomponent of the testsample. METHOD: Method: OECD Guideline201 and EPA CFR 40 797.1050 Type: AcuteStatic GLP: No Year study performed: 1995 Species: Selenastrumcapricomutum Source: In-houseculturesO.riginalflryomATCC, RockvilleM,D Element basis:AJgalcellcounts(cells/mis)p,ecifigcrowthrates. Exposure period:96 hours StatisticMaelthods: Notapplicabldeue tostudyresults. Analyticalmonitoring:Not given Test Conditions: AlgalNutrientMedium: Nutrienmtedium forfreshwatearlgaeperOECD method. This medium servedas thediluenftorallalgaloperations.The pH ofthissyntheticalgalniediumwas 7.9. Stock and testsolutionpreparation:A 1000 mg/L pdmary stocksolution was preparedby dilutin5g00 mg oftestsubstancein500 mL ofstandard algalnetrienntiedium.Aliquotswere then added directltyodilutiownater in testvesselstocreatetestsolutions. Exposure vessels: Not given. Agitation:Not given. Number of replicates:Not given. Initiaclellloading: 1 x 104 cells/mL Number of concentrations: One, plusa blankcontrol Lighting:Continuous Water Chemistry: Notgiven. RESULTS Nominal concentrations: Blank control,1000 nig/L Algal Growth Response ECw values Exposure (Contact) Hours 24 48 72 96 Mean Percent Change in Specific Growth Rate (1000 mg/L Conc.) 13 14 22 18 1 ECSO (mg/L) >1000 >1000 >1000 >1000 Element values are based on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalcomponents alone. CONCLUSIONS The testsubstance exhibitsa 96-hour EC5o forspecificgrowth rateof>I 000 mg/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking= 4. Not alllaboratorydata couldbe located. There isno charactedzafionofthe testsubstance puritya,nalytical measurement ofthe testconcentrationsa,nd confirmationofthe amount of fluorochemicalcomponents inthe solution. REFERENCES This testwas conducted by the 3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number N3281, 1995. OTHER Last changed: 6/26100 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT @IB RE(IUEST NO. N3281 ,@@-@QUESTOR NAME: SBS/SHARI LOUSHIN ,,@EPARTMENT: 3058 i'ROJEC'@,I NO: BILTWENVIR i,al'ERE(,EIVED: 09/28/1995 I-C-201AF LIGHT WATER CONTRACT LAB(S): EXP COMP DAT'E: 12/04!1995 DATE COMPLETED: 01/17,11@)9,6 PROJECT LEAD: TL HATFIELE, PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176 for,toxicity to fish, Daphnia, Microtox and ,,inbe found on lab request N2964 @it,,10 @3,,95 @,@.inda@,t(.l(,stingfor, AFFF products. For,mulation is ,@,'ISM@ 3M TD number given as ZF-0002-0843-7. !@t)'fO,'3,!95 activated on Doc, # s dge resp. 07-2573-9 in ff,omalgae toxicity test: l,,6 96 Mean Percent 24-hrs 13 change 48-hrs 14 in Specific Growth 72-hrs 22 Rate 96-tir's 18 > 10 0 0 > 10 0 0 > 10 0 0 --1000 I)ATE --------------------------- CODE DESCRIPTION RESULT ----------------------- @@9,'04/95 FC-201AF Dark Liquid ,(",--)O-ALGAEALGAE ASSAY - IC50 >1000 MG/L MDL or (950-@C.I.) ENVIRONMENTAL LABORATORY PREPAP,AT)ON WOPY-SHEET FOR FRESHWATER ALGA GROWTH TNHIBITION TES-V'@ T:C-ton-l@-LT-:crSt:@)tL-.ZgvlA r o rr-rI, r@my, -A I LR SEE BELOW PAGI@-: OF DATI:I()A)'qS AN@ IA,'44AI -2Pl PkvftlLu@ni LOL@LL wilci ar LA :D"Dav7:rPnA' A"cl Chitti@nLz, vr@ 10 tn nno g -'Pk (4r@@l e@Zn On. rii- i )C in qrii Lis li-i@ t Test methodok>qy cc>nforms with Ihe followingtwo test@r6tocols: OEC $t2Ol'Alga,'Growth lnhibd@onTest* and Fed. Reg@ Vol.40. No. 797.1050:'Algal Acute To@i6iti;T6sl, STANDARD ENVIRONMENTAL LABORATORY NUTRIENT MEDIUM FOR FRESHWATER ALGAE NUTRIENT CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION STOCK SOLUTION 1: MACRONUTRIENTS 14H,-'1 MgC6 6H20 ,,aC6 2H20 'AGSO, 7H20 ,<H,PO,, 1.5 gL 1 2 gJL 1 8 g/L 1 5 gl0,16 g/L 15 mg/L 12 n)!;fL 13 n-q,L 15 rng/L 1 @6 rrig/L STOCK SOLUTION 2. FE-EDTA Ire--136H,O .','a,EDTA2HO 80 mg/L 100 rr@@ 80 LKPL 100 ug/L ,@,TOCKSOLUTION 3: TRACE ELEMENTS O,BO, kAnC6 4H,O Z @'i"-@1, CoC6 6H20 @CuC6 2H20 Na2MOC)4 2H20 185 mg/L 415 mgfl 3 mg/L 1-5 mgil 0.01 mgil 7 mgfl STOCK SOLUTION 4: NaHC03 185 ug/L 415 ug/L 3 ug[L 1-5 ugL 0,01 ugfl 7 u g@L NAHCO, 50 g/L -,0mg/L ,teriliszteocksolubons1-4 by niembrane fiftrati(o0n,2um). SOLUTION 5: NUTRIENT CONCEti@RATE@ Add 100 mL of STOCK SOLUTION 1; 10 ML of STOCK SOLUTION 2; i,10 mL of STOCK SOLUTION 3; and '@10mL of STOCK SOLUTION 4. LR Mlt@55 N3188 N3194 N3281 SOWT] N 6: STANDAR AwD i 'ofthepH-a NN"utfntW,entaiC@o6*mcegnttZr6ajttdiotno m ,o he med ium after lriobrra*)nonwawnilnir -mll ENVIRONMENTAL LABORATORY FRESHWATER ALGA GROWTH INHIBITION TEST(')"@'@@'. SPECIFIC GROWTH RATE ALGAL SPECIES: Freshwater Green Algae, Selenestrum capricomutum DATE INITIATED: lnlqlqs iL,,o DATE TERMINATED. JPLL34cL4ijiooo ANA TEST SUBSTANCE CONCENTRATION mgil 24 HRS MEAN GR RATE %CFIANGEM 48 HRS MEANGRRATEI %CHANGEM 72 HRS MEANGRRATE %CHANGEC4 MEAN BK CONTROL STD roUTRIENT z.104 M1655 1000 mg,,L N3188 1000mg(L f N3194 0.551 i 15 o.sql s 80 0 1000mg,@ N3281 1000 mg/L L lss DATE/ ANALYST Testmeviodoogy co@rilo(mwsth thefoiiewtngtwo testprotocols OF:CD 0201 "Alga, Growth )Mibit@on Tes@'and Fed Reg VN 40, No 797 10'-W),'AlgalAcute T o xicitTyestA r.irxfat@Cc*owth Rate (u@ - inN InN@l Where N@ tsthe rt)oarm@(iasufed linatco oonsity N tstt)oOinaJcol densityan(i1),i;to lirneof ff)orneasurement. Part) twan r,@)ntiVo,l,iILK)f*an camf,)Wtv@dti(i)@Mea(r,@.,o@t@rVAah-x)v 100