Document 5L9MZ9MK5ayVyBBMMBjw0B9ER

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206CE. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 502. The testsample isFC206CE (2/16/89).Currentinformatioinndicatesitisa mixtureof 0.92% PFOS, 15% diethyleneglycolbutylether,76.09% water,4% urea,1.28% Sultonefoamer, 1.55% sodium octylsulfate1,.05% polyoxyethylene monooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% tolytdazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations ofimpudties. Data may not accurately retlectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static GLP: No Year completed: 1989 Species: Daphnia magna Supplier:In-houseculturesI.nitibarlood stockfrom AquaticBioSystems, Inc,FortCollinsC,O. Analyticalmonitoring: Temperature, pH, and DO Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethod: Not given. Test conditions: Dilutionwater: Carbon-filterewdellwater Dilutionwater chemistry: Temperature: 21 OC Dissolved oxygen: pH: 9.0 mg/L 7.9 Lighting: Not given. Stock and testsolution preparation: Not noted. Exposure vessels: 250 mL Pyrex glass beakers containing200 mL of test solutionand a water depth of6 cm. Number of replicates:one Number of daphnids per replicate:10 Number of concentrations: 4 plustwo blankcontrols Water chemistry during the study: pH range(O-48 hours): 7.9- 8.3 (controelxposure) 7.9- 8.0 (1000 mg/L exposure) Temperature (0-48hours): 20 *C Dissolved oxygen range (0-48hours): 9.0- 9.2mg/L (controelxposure) 3.4- 9.0mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Two blankcontrols1,,10, 100,and 1000 mg/L Element values: 48-hourEC50 = >1,000 mg/L Element valuesbased on nominal concentrations Remards: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206CE 48-hour EC50 was determined to be >1000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets the criterifaorqualitytesting. However, sample puritywas not properlycharacterizedand thestudylacks analyticaclonfirmatioonftheamount offluorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number G1358-2, 1989. OTHER Last changed: 6/26/00 INVIRDI+MAL LAImTloRy DAPMIA 7MICI7Y DATA SMU. LR -44!f aliew 7YPE 2 WEST OXkNISIZ@Wdd/@@ aiEMICAL TESMWIIVI'-- AGE or. TEST oRcA@isis:,c24 h@, ,6V TEST VESSEL: 250 mi P=x R LIlesbeaker TEST SOLLrrIOVNollM: TIME STARTEL_,ZW DATE STARTL7D_y .0 _DATE -DATE RECID-14&&/m R TRFA7@09-.W_ 200ml KKTER DEP711f:irin Oa\,PLEM e/ff2_ DILURIOlNtkTMCarhnn-filtweerlelwdnte (Temp.-gCY--D-.OC..--?,Q_=LpLi,iY!2 y ECso(951Confidence Limits) Test InitialTotal 24 H rs Vessel Conc. Amotumn Total No, pH No. mg/L Added Dead D.O. mg/L 48 Hours otal No pH Dead D.O. mg/L T- Control 21 Cl Ion 2Al Test:-I'engi.@ @7 t initias Date t@. - -7 7@ -7 A r) (2)u:s- te: Is t