Document 5L7GBxXkz4v2pnG6E9ZkBxRQR

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFX-601. (IOctanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was cc 835-34. The testsample isL-9235 (FX-601). Currentinformationindicatesitisa mixtureof 1% PFOS, 12% diethyleneglycolbutylether,81.25% water,2% propane Sultone foamer, 1% sodium octylsulfate1,% sodium decylsulfate0,.1% N-(3chloroallyhie)xaminium chloride0,.8% xanthan gum, 0.8% starch,and 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the ffuorochemical component of the testsample. METHOD Method: Activatedsludge protocolEARNVAS 5-09-84 Test type: Aerobic GLP: No Year Completed: 1984 Analyticalmonitoring: Dissolvedoxygen concentrations Statisticamlethods: Resultswere determined by calculatioonfthe oxygen consumption rate. Test organism source: Activatedsludge mixed liquorfrom theMetropolitan Wastewater Treatment Plant,St.Paul,MN Test condition: Dilutionwater: Deionizedwater. Synthetic sewage: Not given. Reference and testsolutionpreparation: The testmaterialwas added directltyothe dilutiownater on a weighttvolumebasis. Test vessels: Not given. Number of concentrations: threeplusblankcontroli,nduplicate(two studiesrun) Totalsuspended solidsand pH on day of testing:Not given. Element basis:Respirationinhibitiaosndeterminedby oxygen consumption. RESULTS Nominal concentrations: Blank control1,0, 100, and 1000 mg/L. Elementvalues: No inhibiteofrfyecotnactivatseldudgerespiratriaotne was seen at 1000 nig/Lvatha contacttimeof7 minutes. Element valuesbased on nominalconcentrations. Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand notthe fluorochemicalproportionalone. CONCLUSIONS The materialexhibitedno inhibitoreyffectsat 1000 nig/Lover a 7 minute exposure. The respiratiornateofthe testbioreactowras higherthan thatof the blankcontrols. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. The testlacksrecordofmethodology used. The method was notan agency-approved method. The inoculumis notpropedy characterized.There isa lackofcharactedzabonofthetest sample and the studylacksanalyticaclonfirmatioonftheamount of fluorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number B1684,1984. OTHER Lastchanged: 6/26/00 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to-Activated Sludge Mixed Liquor Protocol Reference: EAn/WAS 5-09-'84 Lob request no,: The activated sludge mixed liquor Metro Wastowtor Plant on Sawle - description:-,/, 1)& to -Analyst:' yst s collected from the St. poul/Minneopolis se Preparation of stock solutions. groms Of 3"910 were dlluted,to with, sionlaso reagent water, Da-te--07-preparstlon.. ml '-'TIME C@Ti min. sec. NTWTION OF T. MAT E RIA L li@8T@VATED.SL -on ol ?) I;EMI.TUU'RkR nn Al Ooi oo oo COMKNTSO IKOVMUA(- U-,ICCtt@M 4p, " &-Map 10 100 ke IEO)CX)(L:A)le _tt Acute Toxicity Of 3M Environmntal Laboratory Compound (Substance) tO-Activated Sludge Mixed Liquor Protocol Reference-,EAR/WAS 6-09-'84 Lob request no*'.' The activated sludge mixed 11 Metro Wastowtor Plant on oate:4 1 Analyst:* Was col ected from the St. Paul/Minneapolis Sawle description: Preparationof stock solution! grams of sample were diluttedto with dolonlzed reagent water. Da-t*o@prep&ratlone. mi lm Tm miln@n*1!M.ssoe'c* NTttAiiuN OF TEST MATERI-A TED ISLUDG X R 2on I Iz-,67 n -S.519 3, P3 07 COMWNTS.- Ittifs0.1g;q 09:@ C) toc) 10 cc) C)05. -cf K((Vt) r-orm 10620-8-B PWO SamplDeescription ee OIS5- EnvironmentLaalb- WorkShoot Lob RequestNo--@ Page -of Date: OIZI, lOL4qti MOA4nvtr?z6o I 0:1 41 AMA UAKK) C4 AAP-. t 0 00 Vt4 LP \/AlUe LICQ-C) o miml- --e-@ -1sk (lovk4ymk 4 Kkii@tiim@ IGO FOrm 10620-8-13 PWO Sample Descr'pt' e(4 ego Environmentalpb 7 Work Sheet Ub RequestNo. Date: Analyst: :-7 an JLff t 7y It V14,aU d kk L/ IL;,%-T- oc O-LAZ -0 I'm Y- too 0101(o too