Document 5DLQg4GvKgRK2RNX5QpvO6Lze

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-603F. (IOctanesulfonicadid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC603F. Currentinformationindicatesitisa mixtureof0.5% PFOS, 7% diethyleneglycolbutylether,82.95% water,2% acrylicfoamer,4% Alkylether sulfate1,.45% Xanthan gum, 1.4% calciumacetate,0.07% N-(3Chloroallyl)hexaminiucmhloride0,.5% tdethanolamine,0.05% sodium hydroxide,0.03% cis-1-(3-Chloroallyi)-3,5,7-t1r-iazoan-iaadamantane chloridea,nd 0.05% tolytriazole. The tbiiowingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline201, and EPA CFR 40 797.1050 Type: Acute static GLP: No Year study performed: 1995 Species: Selenastrumcapricomutum Source: In-housecultures.Originallfyrom ATCC, RockvilleM,D Element basis:AJgalcellcounts(cells/mLa)nd growthrate. Exposure period: 96 hours StatisticaMlethods: Not applicabldeue tostudyresults. Analyticalmonitoring:Not given Test Conditions: AlgalNutrientMedium: NutrienntiediumforfreshwateralgaeperOECD method. This nutrienmtedium served as the diluentforallalgaloperations. The pH ofthissyntheticalgalmedium was 7.9. Stock and testsolutionpreparation: A 1000 mg/L primarystocksolution was prepared by dilutin5g00 mg oftestsubstance in500 mL ofstandard algalnutrienmtedium. Aliquotswere then added directltyo dilutiownater in testvesselsto createtestsolutions. Exposure vessels: Not given Agitafion-Not given Number of replicates:Not given. Initiaclellloading: 1 X 104 cells/mL Number ofconcentrationso:ne plusa blankcontrol LighfingC:ontinuous. WaterChemistry:Notgiven RESULTS Nominal concentrations: Blank controland 1000 mg/L 96-Hour Algal Growth Response Exposure Conc. (mg/L) Control 1000 Mean Growth Rate 1.348 0.896 Percent Change in Growth Rate -- 34 Element values: 96-hour IC5o= >1000 n-Q/L Element valuesare based on nominal concentrations. Reversibilityof Growth Inhibition:Not given. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS FC-603F exhibitsa 96-hour IC50 of>1000 mg/L. Thisconcentrationinhibited the algalgrowth rateby 34%. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 4. Not alllaboratorydata couldbe located. Sample puritywas not propedy characterizedand the studylacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number MI 655, 1995. OTHER I Last changed: 6/26/00. ENVIRONMENTAL LABORATORY PREPARATK)N WORKSHEET FOR FRESHWATER ALGA GROWTH INHIBITION TESr' LR SEE BELOW PAGE OF DA 1 AN 4 LA &Iwairy- %t@tea.4 ;=r- 1,A,%4iA] l@ L--Irl?-q -Cul r@u rhpv"iral vAL DCJP LA-IH,4e- MIL*@= U.)1,413 iukimar Sor) L, ---Ad 43inA MadkAv4 zp" DB Pi Cl 11,4nrUldM Pei1;5u erl 2-t34- 4-rA -.PynAA 4 rw tI tiltiCl ILiu y-i- 44-co4- t CTEICV- 1,A (0 JL tol t;,x 16*1 -Ai-e O.nZ j*5 AA 1-t-lIef f%Z %&AI I' Kk!" Ri,< tt+ I Testffwthodokcmoynformwsiththefollowtiwnogtesptrotocols: OECD #201 -Alga.Growth InhibdionTest'and Fed. Reg. Vol.40, No. 797.1050."AlgalAcute ToxicityTeSt".r STANDARD ENVIRONMENTAL LABORATORY NUTRIENT MEDIUM FOR FRESHWATER ALGAE NUTRIENT CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION STOCK SOLUTION 1:MACRONUTRIENTS NH,CL MgC12 6H20 CaC6 2H20 MGSO, -7H20 KH2POI 1.5 g/L 1.2 9A1.8 9/11.5 g/L 0.16 9/L 15 mgfl 12 ffigfl 18 mgL 15 mg/L 1.6 MGIL STOCK SOLUTION 2: FE-EDTA FeCl,-6H,O Na2EDTA -2H20 80 mg/L 100 mg/L STOCK SOLUTION 3: TRACE ELEMENTS 80 ug/L 100 ug[L H,80, MnC6 -4H20 ZnC6 COC6 6H,,O CuC6 2H20 Na2MoO4,2H20 185 ffKjtL 415 MGIL 3 mg/L 1.5 mg/L 0.01 mg/L 7 mgfl 185 ug/L 415 ugil 3 ugIL 1.5 ugfl 0.01 ug/L 7 ug[L STOCK SOLUTION 4: NaHC03 NaHCO3 50 g/L Steriliszteocksolutions1-4 by membrane liftrati(o0n.2um). 50 ff)g/L SOLUTION 5: NUTRIENT CONCENTRATE Add 100 mL ofSTOCK SOLUTION 1; 10 mL of STOCK SOLUTION 2. 10 mL of STOCK SOLUTION 3. and 10 mL of STOCK SOLUTION 4. Make up to.1000mL withMillowe Milh-OTmwater. LR M1655 N3188 N3194 N3261 SOLUTION 6: STANDARD ALGAL NUTRIENT MEDIUM Add 100 mL of theph-adjustedNutrientConcentrateSolutionto900 mL of MIEWE Mil-QwTaMter.InitipaHl ofthe medium afterequilibratiwointhairis- B.O. ENVIRONMENTAL LABORATORY LR FRESHWATER ALGA GROWTH INHIBITION TEST() SPECIFIC GROWTH RATE (2) ALGAL SPECIES: FreshwaterGreen Algae,Selenastrumcapricomutum DATEINITIATED: inlcllgsit, o DATE TERMINATED: -1 (poo ANALYS TEST SUBSTANCE CONCENTRATION mg/L 24 HRS MEANORRATE %CHANGEN 48 HRS 72 HRS MEANGRRATE %CHANGEPTIMEAN MEANGRRATE %CHANGER 96 MEANORRATEI BK CONTROL STD NUTRIENT M1655 1000 mg/L z.104 1.f,48 1.411 N3188 1000 mg/L N3194 1000 mg/L q.I I o.sql 0,+ C@-SOD LO . tol 0.5ZO,"'' N3281 1000 mg/L 1.841 L 155 zz I-ios DATE/ A NALYST Test methodology conformswiththefollowintgwo lostprotocols: InA ig@l OECD 8201 'A$ga.Growth InhibitioTnosr and Fod. Reg. Vol.40,No. 797.1050. 'AlgalAcute ToxirityTesr. Speak Growth Rate (u)- InN,-InN.4W.here N. isthemean measured finaclc4 density;N istheiniticaallldensityand IIsthetimeof thenigasuretnenl Percentchange inGrowth Rate % (k%an ControlValue -Wan Sample ValuoyMean ControlValue x 100@