Document 5DK1YOavxaRa6B49GZm7Mm3qD
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity: Perfluorooctanesulfonyflluoridem;ay also be referredto as POSF, or FX-8. (1-Octanesulfonylfluoride, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluCoArSo-#, 30735-7).
Remarks: The testsubstance isa clearliquiodf uncharacterizedpurity. Itisdescribed as FX-8 (cc842-2)Lot 1 F-10101, L-1407 washed sample.
METHOD
Method: Not given Type: Staticacute GLP: No Year completed: 1984 Species: Pimphales promelas Supplier:Dale FattigB,rady, Nebraska. Analyticalmonitoring:Temperature,pH and dissolvedoxygen. Test substance concentrationnot measured. Exposure period:96-hours Statisticalmethods: Not needed. Test fishage: Not noted. Length and weight: Average length 3.88 cm.
Average weight 0.366 g Loading: 0.61 g fish/ L Pretreatment: None
Test conditions:
Dilutionwater: C@rbon-filterewdellwater, 3M well#2, St.Paul,
MN
Dilution water chemistry:
Dissolved Oxygen: 9.2 mg/L
hardness:
240 mg/L as CaC03
pH:
8.2
Stock and test solution preparation: The testconcentrations were prepared by transferrinagppropriateweights of the test substance directltyo the testchambers. Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: One gallonwide-mouth glassjarscontaining 3L of testsolution. Number of replicates:Two Number of fish per replicate:Five Number of concentrations: Four plusa negativecontrol
Water chemistryduringthe study: Dissolvedoxygen range (0- 96 hours): 6.6- 9.2mg/L (controelxposure) 5.2- 9.2 mg/L (1000 mg/L exposure) pH range (0- 96 hours) 8.0 -8.2 (controelxposure) 7.8 -8.2 (1000 mg/L exposure) Test temperature: ranged 21-22"C
RESULTS
Nominal Element
concentrations: Bk control,10, 100, 500, 1000 mg/L value: 24-hour LC50 = > 1000 mg/L
48-hour LC50 = > 1000 mg/L 96-hour LC50 = > 1000 mg/L
Allelement values based on nominal concentrations
Statisticalevaluation of mortality: Not applicable.No mortalityseen in the any concentrationtested.
Sublethal effects:After48, 72, and 96-hours of exposure, some fish were surfacingat each concentrationtested,but not inthe controls.
CONCLUSIONS
The testsample 96-hour LC5o forfathead minnow was determined to be > 1000 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133
DATA QUALITY
Reliability:Klimischranking2. This study lacksanalyticaldata for determinationof the testsubstance concentrationinthe testsolutionsand determinationof the sample purity.Itisestimated thatthisstudy was run in excess of water solubility.
REFERENCES
This study W2S conducted by the 3M Environment21 Laboratory,St Paul, MN, Lab Request Number B1229-2
OTHER
Last changed: 5/18/00
ENVIRONMENTAL CHEMICAL ENVIRONMENTAL
TOXICITY TEST:
LABORATORY EFFECTS EVALUATION
SUMMARY REPORT
Chemic,alTestedpy
2 ) LZ7 )f 4 Test Tylpe-.-T
0 6@)o-w6fj,----@yf y
E:C50/IC50/GR_50 (95% C.L.)::
4f Z!
mg/1
6?
Test S;pecies:
Test El
L.Ro.tic
Analyst(s)ts
@@(lExpo(@Esxure@sluTrees/@tTest CoonLn@dditions)
E?@posure Type:
Static Static Recirculatory_
Static/Continuous Orbital Shaking
Static Renewal
Flow-Through
Diluent: Aerated Well Water (Initial D.O.g,,,,pg/l; pH
Temp.
Water Hardness:.
Hardness Classification:
Dissolved Oxygen % Saturation:
Contrc)l(s) Lowest-.Experimental Dose tcvtt5& Highes,t Experimental Dose
48-hr PO-
96-hr Iz"
Extended Elpas ure
Lev,els:
Contrcol(s) Lowest-.Experimental Dose 10@@30 HigheE;tExperimental I)oseiO()(-)t4k
48-hr
-a-LL An I
6.0
96-hr P.()
Extended EEpSsure
Test 9
48-hr
96-hr ZZC
Extended En2LSure
OC(
Photol)eriod: Sixteen hours light with a 15-30 minute transition period.
ENVIRONMENTAL LABORATORY AOUATIC.TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST)
Type Tes
Test Organ is mt@@,n
Avg. Wt.
tll
torial Testeda-@5 ourc
7
Date Recld
grams Avg. Size,::Ia@CM No. Organisms per dose
LO
L)
Batch No-L-EY//
Test Solution Volme 3-Liters/All-Glass Tank Tank Dimensions:WIDE-MOUTH I GALLON GLASS JAR
Time Started___/,'C6
Date Started
Date Completed
Dilution Water CaThon-filtered.wellwater (Temp.
0 c, D.0.
m-m& pHjffLZ)
Investip (Full Siipgtn4a)ti
LC so
(95% Confidence
Limits)
Init ank Conc: No. __ASZI
Total Amount A dded
24 urs
41-Hours
Total
D.O.1 I Total
D.O.
No. Dead IpH PPM No.Dead pH Ipps
72 Hours
Total
0.
No.Dead H F;Opm
96 Ho
Total
.0
No Do adTHH @p.a0
Control -!Ln
1")
F, I //)(,/ 1 (-0) k@, L/
iL
C)
n n C)
2Li Li
0
c
Test Temp, C,
Date -
Co@n ts:
c
Z,2- r
Effects other than Mortality
Tank NO.
.Control
Symptoms or Reactions
24-Hr.
ECsn
Total No. Affected
48 Hr.
72-Hr.
96-Hr.
Comments:
ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST)
Type Test. To s t 0 r g an i sa Avg. Wt.
Material Tested"-/'@
A,11,eelt, 0 grams Avg. Size@@'@CM
Date Recld No. Organisms per dose
0/0/) Batc-eN of@7-
Test Solution Volume 3 Liters/Al1-Glass Tank Tank Diwnsions:WIDE-MOUTH I GALLON GLASS JAR
Time Started
Dilul-.ioWnater
Inves tiwl (Fuli.LS igpna
(le OA-1 Date Started f
Carbon-filtered.well water
;LcSo
(Temp..
Date Completed 0 Cob DOOO
(95% Confidence Limits)
@ II pps, PH-z.-2=)
init, ink Conc,, _nAL I
@Control
Totalt
Asoun
@q Added
-@@ -
24 Total No. Dead
urs DOOO
PH pps
F ,@ -/,i '
48 Hours
72
Total
D.0 Total
No.Dead vH ippo-I No.Dead
c, -- a,,1 0
o
3
96 Ho
0 Total
@.00
P,;.;IiNo. adTH- IDS-
c
Lk
C
0 K-)
0 Test To!12, C.-
Init
D at*
Effects other than Mortality
Taiik
No.
s
Conl:rol
toms or Reactions
24-Hr.
EC so
Total No. Affected
48 Hr.
72-Hr.
96-Hr.
4-1 2-R4
E'JVI;;C)I:MFNTAI- LABORATORY
STATUS
ALL RESULTS T-PNTATIVE
R*--:00-qT
LA9 REQUEST NO. B1229
QEQUESTER NA,(E: ANWIT-AR D@-DT: 0535
PROJECT 40: 9970012600 DATE RECEIVW-D: 2-21-94 DESC: FX-S (CC 942-2)
Cn-!iTkACT LA9:
C C'-TRACT LA9 COST:
0
34 E-L HOURS:
46
EST C4PLT DATE: 5-01-84
DATE C04PLETED:
SAMPLE IUMBER
NUMBER DNE.
TWO WILL BE GENERATED
FROM TWO EQUAL VOLUME WASdr-S OF SAMPLE
--------------------------------------------------------------------------------
SA-APLE
DATE
CODE
DESCRIPTION
TEST
RFSULT
------------------------------------------------------------------------------
1
WX-9
S14PL-PREP
COMPLETE
2
WASHED
SA44PLE
96-HR-LC50
#>1000 MG/L
C-BOD-05
SEE REPORT
CwBOD-10 C-BOD-20 SOLUBILITY BIOSORP
SEE REPORT SEE REPORT
SLUDGE-TOX
UPDATED DATA CONTRACT LAB