Document 5DBXZ4zYXXgXy4x5G9gJozyX8
ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH)
TEST SUBSTANCE
Identity:MixturecontainingPerfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-206A. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was cc785-22. The testsample isFC-206A. Currentinformatioinndicatesitisa mixtureof0.92% PFOS, 18.75% diethyleneglycolbutylether,75.44% water,1.83% hydroxyfoarner, 1.75% sodium octylsulfate1,.2% poloxyethylenemonooctylphenylether, 0.06% sodium laurylsulfatea,nd 0.05% benzotdazole.
The followingsummary appliesto a mixture with incompletely characterizectconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemkat component of the testsample.
METHOD:
Method: U.S. EPA 1975
Type: Acute static
GLP: No
Year completed: 1978
Species: Lepomis macrochirus
Supplier:Dale FatfingFishFarm, Brady,NE.
Analyticalmonitoring:Temperature,pH, and dissolvedoxygen. Exposure period: 96-hours
Statisticamlethods: LC50valuesand 95% confidenceintervalwsere
calculatedusingthe U.S. EPA (Duluth)probitcomputer program. Test fishage: Juveniles
Length and weight:
Average length 45 mm
Loading: 0.8g/L
Average weight 0.92g (wet)
Pretreatment:None
Test conditions:
Dilutionwater: Carbon-filterwedllwater Dilutionwater chemistry:
Hardness: 235 mg/L as CaC03 Alkalinity: 221 mg/L as CaC03 Conductivity: 418 pmhos/cm
Lighting:A dailyphotoperioodf16 hourslighatnd 8 hoursdarkwitha 30minute transitonperiodwas maintainedthroughoutthetestingperiod.
Stock and testsolutionpreparation: The testmaterialwas added directly tothe dilutiownater on a weight/volume basis. Concentrations dosing rate:Once.
StabiliotfythetestchemicalsolutionsN:otnoted. Exposurevessels:Glassaquari(a35x3Ox2cOm)containi1n6gL oftest solution. Number of replicates:2 Number offish per replicate:10 Number of concentrations: fiveplusa blank control Water chemistry during the study:
pH range (0-96 hours): 7.9 - 8.0 (controelxposure) 7.5- 7.9(1350 mg/L exposure)*
Temperature range (0-96hours): 17 - 18 OC Dissolved oxygen range (0-96hours):
5.8- 9.9 mg/L (controelxposure) 0.1 - 7,.3mg/L (1350 mg/L exposure)* 1350 mg/L (intermediatceoncentratione)xposure valuesused because the highestthreeconcentrationsused resultedintotal mortalityby 48 hours.
Remarks: Very low dissolvedoxygen concentrations(0.1- 1.8rng/L) observed inthe exposure concentrations(exceptforthe controlb)y 48 hours.
RESULTS
Nominal concentrations: Blank control1,000, 1350, 1800, 2400, and 3200 mg/L (listeidntextas g/L)
Element values: 96-hourLC50 = 1300 (1100- 1400)mg/L (Duplicate1) 1200 (1000- 1300)mg/L (Duplicat2e)
Element valuesbased on nominalconcentrations.
Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsample 96-hour LC50 range forbluegilslunfishwas determined tobe 1200 - 1300 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking2. Testingmeets the criterifaorqualitytesting. However, sample puritywas notproperlycharacterizedand the studylacks
I
analyticaclonfirmationoftheamount offluorochemicalproportioninthe soluton. REFERENCES Thisstudywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, 1978. OTHER Lastchanged: 6/26/00
3m
Fo:m 6747-11 A
TO: TECHNICAL
TECHNICAL REPORT
COMMUNICATIONS
CENTER - 201-2CN
SUMMARY
2=7;l
8
(Important- Ifreport isprinted on both sidesofpaper, sandtwocopiesto TCC.)
Division Project Report Title
Environmental
-Commercial
Laboratory
Chemicals
(EE &
FC-206A cc 785-22:
Results of
To
Authoris)
D. L. Bacon
A. N. Welter
Notebook Reference
SECURITY
rLO]pen
0 Cfosad
KEYWORDS:
(Selecterms from 3M
Thesaurus. Suggest other
applicable terms.)
EE & PC-Div. (Environmental-
Lab) Toxicity '@Aquatic)
CURRENT OBJECTIVE:
To determine
the acute
(Lepomis macrochirus) of FC-206A cc 785-22.
PC)
Aquatic
96-Hr.
@'D
op t. N Ymber@
0535
Project 4umbe -
9970012600
Floport Tu-m-S;r-
LC,--, Te. t-
003
IICAL
REGR IY S
rnployee umber i
09362
-d..,.ciuaingg.;overshost
6
-
NowCh-emRiecpaolrsted
0 Yes
No
LC
value for the bluegill sunfish
expgged to varying concentrations
REPORT ABSTRACT: alert3M'ers to Company
(2R0&0-D2.50
words) This abstractinformation isdistributedby tie Toc
iicalCommunicat-011.1 Center
The acute static 96-hr
LC
for bluegill
sunfish
macrochirus)
exposed
to' va@9ing concentrations
of
FC-206A cc 785-22 ranged from 1.2 to 1.3 g/l.
(Lepomis
InformationLiaison Initials: - K u)
FC-206A
November 2, 1978
CONCLUSIONS
In a replicated static 96-hour LC aquatic test, the calculated
LC
values for bluegill sunfish ?Pepomis macrochirus)
were 1.2
angol.3g/l respectively. Materials possessing an LC of this order of magnitude are considered to present an insiirificant
hazard based on the NIOSH aquatic toxicity criteria.
INTRODUCTION
The test substance FC-306A cc 785-22 is an amber liquid. Tests performed on this material evaluated its aquatic toxicity and the data generated form the basis of this report.
METHODS
A. Aquatic Testing
The test protocol utilized for this study was modeled after that described by US/EPA 1975.
B. Acclimation Procedure
The bluegill sunfish (Lepomis macrochirus) were obtained from a private hatchery.* Stock fish were held in a fibergla,5s holding tank filled with carbon-filtered well water at 14-15 0 C. A daily photoperiod of 16 hours light and 8 hours dark with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed "Tetra-Min"# daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated for 15 days prior to testing.
An acute short-term (96Lhour) static bioassay was performed on FC-206A. Carbon-filtered well water of known composition was used as the diluent (Attachment 1). All glass aquaria (35 x 30 x 20 em) containing 16:1 of diluent or diluent plus toxicant comprised a study chamber. Ten blugills, uniform in size, were tested at each chemical concentration. Test fish were randomly allocated to each test chamber within 30 minutes following to6icant addition. Bluegill experiments were conducted at 17 C. Mortality, temperature, dissolved oxygen level and pH of all test solutions were recorded. General observations relative to behavioral changes were similarly recorded when appropriate.
These studies were performed in replicate using the following FC-206A (cc 785-22) concentrations: 1.0, 1.35, 1.8, 2.4, and 3.2g/l. LC values with 95% confidence limits were calculated using the UG?EPA (Duluth) probit computer program. Organisms used in this study were considered to be generally healthy and free of disease.
*Dale Fattig Fish Farm, Brady, Nebraska. #Commercial Fish food of known composition.
FC-206A
-3-
November 10, 1978
RESULTS
The bluegill sunfish (Lepomis macrochirus)
used in this study
had an average weight of .92+.36 grams and a length of 4.5+0.6 cm.
The loading factor for these studies was 0.6 g/l below the-maximum
recommended loading factor of 0.8 g/l (@). Temperature and pH
ranges throughout this study were 17-18 C. and 7.6-8.0 pH units respectively.
Dissolved oxygen values decreased sharply 48-hour post initiation of this study. Test aquaria solutions originally were clear
(normal DO levels), became straw colored (low DO), and at termina-
tion of this study had assumed a grayish-white color. This latter
color change was accompanied by a relative increase in the dissolved
oxygen content. Although the DO levels determined were in the
range of 0.1-0.3 mg/l, there was no apparent increase in mortality
due to what would be assumed to be a general hypoxic situation.
The absence of an increased mortality attributable to low dissolved
oxygen content relationship is taken as presumptive evidence that
the calculated
LC 50
values are reflections
of test chemical induced
mortality, per se.
In this replicated study, the 96-hour LC values were 1.2, range
1.0-1.3 and 0.3, range 1.1-1.5 g/l respe8?ively. These values
translate to LC values of 1200 and 1300 ppm. Chemicals possessing
an LC
alue irothis range are considered to be an insignificant
hazaraotov the aquatic environment (2).
REFERENCES
(1) US/EPA - Methods for Acute Toxicity Test with Fish, Macroinvertebrates and Amphibians, Ecological Research Series 660/3-75-009, 1975.
(2) Registry of Toxic Effects of Chemical Substances, 176 Edition, Christensen, H. E., and E. J. Fairchild, Eds. p. XV and Ci, U. S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, DC, June, 1976.
A c I I, /.
ANW/cen Attachments
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ENVIlIONt@IL-'N'I'AELN(;INL-L-'RING LALiOl,A*1'01@Y AQUATIC TOXICITY DATA SHEE'L-
Type Test
,rest Organism
Avg. iVt. , c 3:
Test Solution Volume-
Time Started
c.,@
Dilution Water Carbon
Investigator (Full Signature
grams
i%laterial Tested Vendo Avg. Size
Liters/Tank No. Date Started
filtered
well (Temp. water
11:
0 C
LC 50 (95% Confidence
c
Date Recfd_2L_@@ CM.
Bitch No
Organisms per dose
Date Completed D.O.
Limits)
o !,.11-"7i
pik 7 iI
I
Conc. Control
-foat-I kmount Added
4 -Hour.s--
Total
D.O.
No. Dead Pli ppm
Total No.Dead
72 :)urs
96-flours
D.O. Total
D.O. Total
D.0
pH
m No.Dead
H m No.Dead Ff
6r
7
Test Temp. C
C-1
Signature & Date
WA-LZ@V /10/if/7r
A;ju),,
Comments:
Effects other than Mortalit),
-7C@onc. mg/i
Symptoms or Reactions
Control
24-Hr.
L4
Total No. Affected 48-flr.
72-llr.
Sj--ature Date
Comments:
lo
(Oti en)4
idi-a17 lr
96 -1Ir.
[@NV)IZONNII:N'I'ALEN(;[Nl-'I:ItlNc; AQUATIC TOXICITY DA'I'A c-IfEE'I*
Type Test
@laterial 'Tested :-C
Lot c, 7@.6--2 7-
Test Organism
Vendor
Date Rcctdt-/L/2,i/,7,]@3atcli No
Avg. lyt.
3
@rams
Avg. Siz 41
Test Solution Volume
// .(1/(; Time Started
Dilution
/,P,Vcu Water Carbon
Investigator (F7ull Signature
16
Liters/Tank
No.
Date Started
@fi.ltered well (Temp. water
0 __@L
LC So (95% Confidence
Orga nlsms per dose Date Completed
-, D. 0. Limits)
/c: Pil 7.j-
Conc. --fota-1 kmount
;z@:kjftg/l. Added
Control
.
4-Ho ur s
Total
D.O.
No* Dead -Pil- -Rpm
48-Hours
Total
No.Dead
H
D.O. m
72-Hours
Total
D.O.
No-Dead -LH ppm
96-flours
Total
D.C
No.Dead li
@--C)-i
o
.0
.0
6y 10
Test Temp. Oc
c
Id
Signature & Date
p
Comments:
Effects other than Mortalit),
Conc mg/l*
S)'mptoms or Reactions
Control
ti
24-Hr. 0
Total No. Affected 48-ffr.
72-lir.
0
0
I96-lir.
Si@nature
Date
Comments:
An 411
jot It 1-v1