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ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:PerfulorooctanesuIfonylmaido(ethyla)cetate;may alsobe referredtoas PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-1 09, or FC-109-X. (Glycine,N-ethyl-N[(heptadecafluorooctyl)sulfonpyolt]a-s,siumsalt,CAS # 299151-7) Remarks: The testsample isFC-128.Currentinformatioinndicateistis a mixtureof86% testsubstanceand 14% C4-C7 fluoroalkcyalrboxylate compounds. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1974 Species: Pimephalespromelas Supplier:Not noted. Analyticalmonitoring:pH and DO Exposure period:96-hours Statisticamlethods: Graphed Test fishage: Not noted. Length and weight: Average length Average weight Pretreatment: Not noted. 2 inches 1.5g Test conditions: Dilutionwater: Carbon Filteredcitywater Dilutionwater chemistry: Not noted. Stock and testsolution preparation: Individualweights Concentrations dosing rate:Once Stabilityof the testchemical solutions: Not noted Exposure vessels: Type not noted. 16L volume. Number of replicates:one Number of fish per replicate:Not noted Number of concentrations: fiveplus a negative control Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 5.0 - 6.1 mg/L (controlexposure) 4.0- 5.7mg/L (40 mg/L exposure)* pH range (24- 96 hours): 7.0- 7.1 (controelxposure) 6.7- 7.0 (40mg/L exposure)* 40 mg/L testgroup data given because totalmortalitoyccurredin the 50 mg/L testgroup by 72 hours and alldata was notcollected. Test temperature (24 - 96 hours): 72 to 74*F RESULTS Nominal concentrations: Bk control,10,20,30,40, and 50 mg/L Element value: 96-hourLC50 = 34 mg/L (C.In.otcalculated) Allelement values based on nominal concentrations CONCLUSIONS The testsample 96-hour LC50 forfatheadminnow was determined to be 34 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking3. This studylacksdocumentation and informationon the methodology. Sample puritywas notproperly characterized. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, completed on May 18,1974. OTHER Lastchanged: 5/18/00 FORM 10620-PWO .,cc:LAB REQUEST BOOK - 4@2-5W Water and Wastewater REQUEST FOR LABORATORY WORK TO: ENVIRONMENTAL ENGINEERING LABORATORY- Requestor File: LabRequest NO. BUILDING 53-6N Lab Personnel Name: Request Date ProjecNto.- Prob.No Code Description: Sample Date: Date Needed: HoldSampleFor-Days EstT.ime:- Hrs. Date Rec'd.SpiD le: AnaiN WflF y Date Compl eted: B :s Hrs.Spent: Sample Description Total Hardness,mg/i as CaC03 Calcium Hardness,mg/i as CaC03 7'-PA"lkalinitym,g/l as CaC03 "M" Alkalinitym,g/i as CaC03 pH Sulfate,mg/i as S04 Nitrate,mg/i as N03 lfh-loridem,g/l as Cl Silica,mg/l as Si02 Fluoride, mg/i as F Ortho Phosphate, mg/l as P Total Phosphate, mg/i as P Total Chromium, mg/l as Cr Rex. Chromium, mg/l as Cr Total Cyanide, mg/i as CN Ferrocyanide, mg/i as CN Iron, mg/i as Fe v Manganese, mg/l as Mn Surfactant, mg/l as LAS Phenol, mg/i BOD, mg/i dOD, mg/i (Short, Long, Dilute) Total Suspended Solids,mg/i N7o-latilSeuspended Solids, mg/l Turbidity, mg/l Silica Total Solids, mgA Total VolatileSolids,mg/l Conductivity, mhos Total Bacteria,No./100 ml fo-talColiform, No./100 ml Fecal Coliform, No./100 mi Fecal Streptococcus, No./100 mi Ammonia Nitrogen, mg/l as N Viedidahl Nitrogen, mg/l as N Oil and Grease, mg/l, C.CNU - Comments'@J White- Environmental Enara. Lab Canarv-EnvironmontsIE-nora. Lob Pink -Environmental Enarn.ish C'.nlei*nrnet-ReoudetersCoov 3M carbaniess Paper 740101. EWIROSWMA L ENGINEERING IABORA7%)RY AQUATIC 'TOXICITY NM SHEET -Type Test A@711/1 <1 Test Ors'an Date Started Tiii Started Dilution Water- C Material TestedLFr,-Ia.T Avg. Avg. Weigh't 5q Size_ -Z Dat CoWleted Plotted L4CS 7y- Ir oi/P L7/ 24 hrs D.0 Cone,-,-! Survival- PH 'Ppm 40 Iii3 D.0 Survival PH mn 72 hrs D.0 Survival PH ppm 96 hrs D.0 Survival pH ppm 15@ 00 '7. @-iz 70 50 76 7-0 6*f"'5o V)o &g 415, 70 @.1.5.7 too 6.?17 A/. - 0' s-I 710 5.2- 6o. 6.(7,41o 1 5.-7 1 5.o 3c) C) ao ALO D-o 6155 'kier!AeTv"rAtur AlS.CL... 7:@ 100-' LC so Wculation f 71: 1- 14 7, A4 0- 0 j lo 0 1'6 -@o 30 40 SO 60 70 80 90 100 % Survival