Document 50E61Jm0kZk276v2jxXdvkBVN

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC116,FC-1 26,FC-1 69,or FC-143. (Octanoicacid,pentadecafluoroammonium salt,CAS # 3825-26-1) Remarks: The 3M productionlotnumber was 37. The testsample isFC143. It'psuritywas notsufficientclhyaracterizedt,hough currentinformation indicatesitisa mixtureof96.5 - 100% testsubstance and 0 - 3.5% C6, C7, and Cg perfluoroanalogue compounds. METHOD: Method, USEPA 660/3-75-009,1975 Type: Staticacute GLP: No Year completed: 1980 Species: Pimephalespromelas Supplier:DaleFattigofBrady,Nebraska. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Probitanalysis Test fishage: Not noted. Length and weight: Average length 3.6cm Average weight 0.40 g Pretreatment: Not noted. Test conditions: - Dilutionwater: Carbon-filterewdellwater. Dilutionwater chemistry: Temperature: 170C Dissolved Oxygen: 10.0 ppm pH: 8.0 Test conditionsremarks: A dailyphotope@od of 16 hours lightand 8 hours dard,witha 30 minute transitiopneriodwas maintainedthroughoutthe acclimationand testingperiod. Stock and testsolutionpreparation: Not noted Concentrations dosing rate:Once Stabilityofthe testchemical solutions:Not noted Exposure vessels: 35 X 20 X 25 cm deep glassaqua@ums containing16 litertsestsolution. Number of replicates:two Number offishper replicate:20 Loading rate:0.50 g/L Number of concentrations:fiveplusa blankcontrol Water chemistry during the study: Dissolvedoxygen range(24- 96 hours):4.0- 5.0mg/L pH range(24- 96 hours):7.9- 8.0 Testtemperature(24- 96 hours):18-19*C RESULTS Nominal concentrations: Bk control5,60, 650, 750, 870, and 1000 mg/L Element value: 96-hour LC50 = 766 (743 - 789) mg/L Element value based on nominal concentrations. CONCLUSIONS The testsample 96-hour LC50 forfatheadminnow was determined to be 766 mg/L witha 95% Confidence Intervaolf743 to789 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking2. Testingmeets the criterifaorqualitytestingat the time itwas conducted. However, sample puritywas not propedy, characterizedand itlacksanalyticaclonfirmationoftestsubstance concentrations. REFERENCES 3M TechnicalReport Summary, AquaticToxicitTyesting:FC-143 (Lot-37) L.R.5625 S, Report Number 037, ProjectNumber 9970012600, M. T. Elnabarawy, May 8, 1980. ,OTHER Last changed: 5/24/00 Form6747-11-A TECHNICAL REPORT TO:TECHNICALCOMMUNICATIONSCENTER - 201-2CN SUMMARY 5/8/80 .)mporantI-freporitsprintoendboM sidesofpapwenrd,twocopietsoTCC.) Division Environmental Laboratory (EE Project PC), St. Paul, Minnesota Commercial fqepon Title Chemicals Aquatic Toxicity Testing:/-,-F@-c-143 (Lot-37) L.R. 5625 S To Owt. Numbw 0535 rrcioctnumsw- 9970012600 F,@oortNumb4w 037 E. A. Reiner Author($) c M. T. Elnabaravy Notebw* Rtforerne 50012: 23 SECURITY 0, Sd Open 2-3E 13 Closed (Special Authorization) 3M CHEMICAL REGISTFTY KEYWORDS: (Selem torrm tram Thesaurus. SugMt applicable WmL) 3M other CURRENT OBJECTIVE-. To determine the acute aquatic*LC to varying concentrations of FC-lig F-mplOYMSHUMberiol 46981 Na at FeW IncludingCoversh"t 5 Now ChwnkWs C3 Yo Reported g) No following exposure (lot-37). EE & PC - Div. (Env. Lab) xicity @Aquatic) REPORT ABSTRACT:(200-2w5o0rdsT)hisabstraicntformatiisdoinstribbuyttehdeTechnicCaolmmuWm=tionCsenteiro alwt3M'ortsoCompanyR&D. The acute static aquatic 96-hour LC50 values obtained for fathead minnows (Pimeghales promelas) following exposure to FC-143 in replicated experiments were 776(743-812) and 754 (723-787) mg/l. The combined 96hour LC50 was 766(743-789) mg/l. Data generated include LCso values,with 95% confidence limits. Bacon welter lnformatiogLiaison Initials: CONCLUSIOTIS In these replicated 96-hour LC50 aquatic tests, the calculated values for fathead minnows (Pimephales promelas) were 776 and 754 mg/l. Materials possessing an LC50 Of this order of magnitude are considered to be practically nontoxic based on the NIOSII criteria for aquatic toxicity interpretation. (2) INTRODUCTION: The test substances is a white fine powder, soluble in water at room temperature. Tests performed on this material evaluated its potential aquatic toxicity and the data generated form the basis of this report. ACCLIMATIO[G At4D EXPERIMEt4TAL DESIG[I: The test protocol utilized for this study was modeled after that described by USEPA (1975). (1) The fathead minnows (Pimephales promelas) were obtained from a private hatchery (a). Stock fish were held in a flow through system fiberglass holding tank filled with carbon-filtered wellwater at 14-16 degrees C. A daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min (b) daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated to test temperature for 2 days prior to use in assays. Twenty (20) fathead minnows, uniform in size, were tested at each chemical. All-glass aquaria, 35x2Ox25 cm containing 16 liters of diluent plus toxicant comprised a study chamber. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. The fathead minnow experiments were conducted at 19+2 degrees C. mortality, temperature, dissolved oxygen level, and pH of all test solutions'were measured at 24-hour intervals or until total mortality had occurred. General observations were recorded relative to swimming pattern and behavioral-changes induced by exposure to the test chemical. The acute short-term static bioassays were performed on FC-143 using carbon-filterea well water of known composition and constant quality as the diluent ('L"able1s,2). The 96-hour fish studies were performed in replicate using the following FC-143 logarithmic concentrations: 560, 650, 750, 870, and 1,000 mg/l. LC50 values with 95% confidence limits were calculated using the probit program and 3M TRAC Computer system. Monitoring equipment used included ASTM thermometers, Orion teinperature--coinponsating p[lmeter, a Yellow Springs dissolved oxygen meter, and a Taylor temperature chart recorder. a) Dale Fattig Fish Farm, Brady, Nebraska. b) Commercial Fish Food of known composition. RESULTS: The fathead minnows (Pimephales promelas) used in these studies had an average weight of-U.-4-grams and a length of 3.6 cm. The loading factor was 0.5-g/l; the maximum recommended loading factor is 0.8 g/l (1). Temperature, pH, and dissolved oxygen values and ranges obtained during this study were: 19+ 10 C., 7.9 - 8.0 pilunits', and 96-hour dissolved oxygen value-s ranged from 4.0 - 5.0 mg/l. These latter values calculated out to a minimal oxygen saturation of 45-56%. In this replicated study, the fish 96-hour LC50 values were 776 and 754 mg/l. Chemicals possessing an LC50 value in this range' are considered to be practically nontoxic to the aquatic environment (2). The data reported herein are based on studies.Performed by M.T. Elnabarawy. Accompanying data sheets comprise original data. A copy of this report is filed in the Environmental Laboratory Archive System, Building 2-3E. It should be noted that the generated results were reported in the absence of information relative to the "purity" and the ".stability"of the chemical substance FC-143 (Lot 37). The reported LC50 values were calcuated on the basis of the "observed mortality rates" and in the absence of the actual measured final concentrations of FC-143 (Lot 37) in test solutions. REFERENCES: (1)'US/EPA - Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians, Ecological Research Series 660/3-75-009, 1975. (2) Registry of Toxic Effects of Chemical Substances, 176 Edition, Christensen, H. E., and E. J. Fairchild, Eds., p. XV and Ci, U.S. Department of Health, Education and Welfare; Ilational Institute for Occupational Safety and Health, Washington, D.C. (June, 1976). TABLE 1 SAMPLE DATE: .SAMPLE TAKEN: WELL WATER 4/30/80 3M Bldg.. 2-3E QUALITY SAMPLE DATE 4/30/80 (TAKEN FROM TANK #4) PARAMETER pit Residual Chlorine Ammonia Nitrogen Conductivity Alkalinity Total Solids Total flardness Total Fluoride Dilute.COD Total Coliform Standard Plate Count Aluminum as Al* Arsenic as As* Boron as B* Cobalt as Co* Copper as Cu* Chromium as Cr* Cadmium as Cd* Iron as Fe* Nickel as Ni* Lead as Pb* Silver as Ag* Zinc as Zn* Mercury as Hg* PCBIS** Total Organochlorine** Pesticides CONCENTRATION 7.6 <0.02 mg/l <0.1 mg/l as N 400 umhos/cm at 250C 226 mg/l as CaC03 378 mg/i 272 mg/l as CaC03 <0.02 mg/l 1.3 mg/l negative 42 per ml 0.01 mg/l <0.005 mg/l <0.2 mg/l <0.002 mg/l 0.018 mg/i <0.005 mg/l <0.002 mg/l 0.022 mg/l 0.005 mg/l <0.005 mg/l <0.002 mg/l 0.13 mg/l <0.2 ug/l <0.50 ug/l <0.50 ug/l ENV. LAS .WATER QUALITY BOOK REF. 8 9 8 9 8 8 8 9 9 10 10 14 14 14 14 14 14 14 14 14 14 14 14 14 36 36 Total <0.50 ug/l 36 Organophosphorus** Pesticides Sample Date 3/31/80 **Sample Date 2/26/80 TABLE 2 ENVIRONMENTAL LABORATORY, BLDG. 2-3El ST. PAUL, MINNESOTA FIRST QUARTERLY REPORT, 1980 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING PARAMETER TOTAL HARDNESS* .as mg/l CaC03 TOTAL "M" ALKALINITY* as mg/l CaC03 ELECTRICAL SPECI5IC CONDUCTANCE* as umhos per em at 250C TOTAL SOLIDS* as mg/l pH** STANDARD PLATE per 1.0 ml COUNT*** January 24 February 26 March 31 260 268 264 223 220 225 380 370 400 350 408 402 7.6 7.6 7.6 29 30 19 AVERAGE (+ S) 264 + 4 223 + 3 383 + 15 387 + 32 7.6 + 0 26 + 6 CRITERIA OF CONSTANT QUALITY Monthly ranges should be less than 10% of their respective #10, ASTM Committee E-.35 on Pesticides). averages. (Draft Monthly range of pH should be less than 0.4 unit. ttee--R35on Pesticides). (Draft #10, ASTM Commi- Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017, page 101.)