Document 4vayKNayORVGnRGJ6N46ro0Qp

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 628. The testsample is FC-600 (US). Currentinformatioinndicatesitisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutylether,1.00% sodium octyl sulfate2,.00% propane Sultonefoamer, 1.00% sodium decylsulfate, 0.85% xanthangum, 0.1% N-(3-chloroallhyeix)aminium chloride0,.80% starch,and 0.05 % benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentration of impurities.Data may not accurately reflectthe toxicityof the fluorochemicalcomponent of the test sample. METHOD: Method: Not noted. Test type:Acute static(Range-finding) GLP: No Year Completed: 1983 Species: Pimephalespromelas Supplier:Commercial supplierinBrady,Nebraska Analyticalmonitoring:Temperature,pH, DO Exposure period:96-hours Statisticamlethods: NotapplicableN.o mortalitaythighest concentrationtested. Test fishage: Juveniles Average Length and weight: Length = 3.6cm Weight = 0.39 grams Loading: 0.65 g/L Pretreatment: Not noted. Test conditions: Dilutionwater: Carbon-filterewdellwater,St.Paul,MN. Dilutionwater chemistry: pH: 8.2 Dissolved Oxygen: 9.6 mg/L Temperature range: 180C Lighting:Not noted Stockand testsolutiopnreparationT:estsolutiownesrecreatebdy directweights additions. Concentrations dosing rate:Once Stabilityofthe testchemical solution:Not noted Exposure vessels:Wide-mouth one gallonglassjarscontaining3 liters of testsolution. Number of replicates:One Number of fishper replicate:5 Number of concentrations: Four plustwo blankcontrols Water chemistry during the study: pH range (0- 96 hours): 7.9- 8.2 (controelxposures) 7.7- 8.2 (1,000mg/L exposure) Dissolved Oxygen range (0- 96 hours): 5.8- 9.6 mg/L (controelxposures) 0.7- 9.6 mg/L (1,000mg/L exposure) Temperature range (0- 96 hours): 18 - 21OC RESULTS Nominal concentrations: 2 blankcontrols,1,10,100,and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L Element value based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuereportedappliestothatmixtureand not thefluorochemicalproportionalone. CONCLUSIONS The FC-600 96-hour LC50 forPimephales promelas was determined to be >1,000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. The studymeets allthe criterifaorquality testing.However, the sample puritywas not propedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemical proportioninthe solutions. REFERENCES This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 8875, 1983. OTHER Last changed: 6/28/00 %am-. Type Teste4@Zt Test Organ is MA:;@@ Avg. Wt. ENVIRONMENTAL IABORATORY AOUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDI'NG TEST) LR 7 &Ad""Ource Material Tested km Da to Recld. grams Avg. Siz*5&CM No. Organisms per dose Lot atch No. _5 Test Solution Volume 3 Liters/All-Glass Tank Time Started,!Sl@Date Started Tank Dimnsions:WIDE-MOUTH Date Completed I GALLON GLASS JAR Dilution Water Carbon-filtered.wellwater (Temp. /6 oc. Investigator (Full Signaidre)L- LCSO (95% Confide4l; D.O. a-1/ts) Mo L pHjff4Z.) init. 3nk *zonc. control Total Amount Added 2-4 Hours 48 Total D.O. Total No. Dead pH ppo I No.Dead urs D.O. PH I pps 7Z Hours Totalad LH f;l0; No.Do 16 Ho s otal 0, @NNo.De aDdIH @;m notIL i-z In L-z3L &j /pq, 0 ELI 42il a TestTair.O-C.TnLtlais4 Date Co@nts:", Effects other than Mortality Tank No --Symptoms or Reactions control 24-Hr. ECSO Total No. Affected 48 Hr. 72-Hr. 96-mr. If//7,/"C-