Document 4v1Mr53kQp0OvagBB2BGKLO1V

Z,,J5 RCV 13 79 mm (Tftei'& KEo teo; E. I. du P o n t de N em o urs & C o m p an y IN C O R P O R A T E D W i l m i n g t o n . D e l a w a r e 19898 POLYMER PRODUCTS DEPARTMENT E X P E R IM E N T A L s t a t i o n PERSONAL AND CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File l.C. - 353 - N11400 - 269 ^/S.2 Z(o~ 15SO May 20, 1981 DR. W. J. REINHARD F&F FAIRFIELD, CT. ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 811-595; PRAL Nos. 81-1758-1762; Notebooks Nos. E22514,E26238) As requested in your letter of 4/23/81 to L. J. Papa, the five blood samples submitted then have been analyzed for perfluorooctanoate (Cg) . Results and sample identification are given in the attached table. As noted there, th analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of a* 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Key Words: Perfluorooctanoic Acid Perfluorooctanoa te Blood Analysis GC S. S. Stafford There's a world ol things we're doing something aDout 000085 EXP000016 EID713830 TABLE I V CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sample PRAL No. 81-1758 81-1759 81-1760 81-1761 81-1762 Date Sampled S.S-No. 4/23/81 049-16-3890 4/23/81 244-92-6368 4/23/81 049-40-0400 4/23/81 042-26-5501 4/23/81 045-40-8095 GC Analysis _______________ (b) Date Analyzed [Cr ], pg F/g blood 5/5/81 n .d . 5/5/81 5/5/81 5/5/81 5/5/81 n.d. 0.011 0.048 0.012 (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is. + 10% relative standard deviation. The lower limit for quantitation is 0.007 ygF/g. The detection limit is ".,0.004 ugF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] < 0.004 ppm, which cannot be distinguished from reagent background. *R E D A C T E D * EXP000017 EID713831 000086