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ACUTE TOXICITY TO FISH
- /t
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TEST SUBSTANCE_______________________________________________
Identity:
Perfluorooctanesulfonate, Lithium salt; may also be referred to as PFOS Li salt, FC-94, or FC-94-X. (1-Octanesulfonlc acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, lithium salt, CAS # 29457-72-5)
Remarks: Test sample was taken from 3M production lot #1. The test sample is a mixture of the test substance in water (approximately 24.5% test substance and 75.5% water). No calculations were made to adjust for the actual concentration of the test substance in the test sample.
V*
M E T H O D ___________________________________________________
Method: Not noted. Type: Static acute GLP: No Year completed: 1994 Species: Pimephales promelas Supplier: Aquatic Biosystems Inc., Fort Collins, CO Analytical monitoring: pH and DO content Exposure period: 96-hours Statistical methods: LC50 values calculated by Trimmed Spearman -
Karber. Test fish age: 79 days. Length and weight: Average length = 2.1 + 0.3cm, Average weight =
0.069 + 0.03 g Loading: 0.69 g fish / L Pretreatment: None
Test conditions: Dilution water: Carbon filtered well water Dilution water chemistry: pH: 8.4 DO: 8.1 mg/L Stock and test solution preparation: A primary stock solution was prepared in dilution water to yield a test sample concentration of 400 mg/L. All test solutions were made by diluting the appropriate amount of stock solution with dilution water to make 1 L of solution per concentration. Stability of the test chemical solutions: Not noted. Exposure vessels: 2 L glass beakers Number of replicates: two. Number o ffis h per replicate: ten
001526
Number of concentrations: six plus a negative control Water chemistry during the study:
Dissolved oxygen range (0 - 96 hours): 6.0- 7.2 mg/L (control exposure) 4.8-7.9 mg/L (56.0 mg/L exposure)
pH range (0 - 96 hours) 8.0- 8.4 (control exposure) 8.0- 8.4 (56.0 mg/L exposure)
Test temperature range (0 - 96 hours) 19.2-19.5C
RESULTS
Nominal concentrations: Bk control, 3.2, 5 .6 ,1 0 .0 ,1 8 .0 , 32.0, 56.0 mg/L
Element value: 96-hour LC5o= 19 mg/L (95% C.I.: 16-24 mg/L) Mortality of controls: None Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample.
CONCLUSIONS_____________________________________________ .
The test sample containing 24.5% Perfluorooctanesulfonate, Lithium salt exhibited a 96-hour LCsofor fathead minnow of 19 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY ___________________________
Reliability: Klimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions; and determination of the sample purity.
REFERENCES____________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number M1018, 3/25/94.
O T H E R ________________________________________________
Last changed: 5/2/00
001527
ENVIRONMENTAL LABORATORY
LR: M 1018 Date: 3 / 2 5 / 9 4 Analyst: JBD
Plmephales promelas 96-hour Toxicity Test Data Summary
SAMPLE: FC-94-X (F-11367, Lot #1)
Sample Cone. (mg/L) 3.2 5.6 10 18 32 56
s/
24 hours 0 0 0 0 0 0
Percent Mortality
48 hours
72 hours
00
00
00
0 10
0 50
20 60
96 hours 0 5 15 50 65
100
24 h LC50 (95% C.I.) 48 h LC50 (95% C.I.) 72 h LC50 (95% C.l.) 96 h LC50 (95% C.l.)
> 56 mg/L > 56 mg/L 36 mg/L (15-85) 19 mg/L (16-24)
NOTE: Spearman-Karher method used to calculate those LC50's which could be estimated. (72 h LC50 required 40% trim, 96 h LC50 did not require any trim)
001528
ENVIRONMENTAL LABORATORY
lr r r n o i ft
96-HRACUTETOXICITYTOFATHEADMINNOW, PtMEPHALES PROMELAS
TYPETEST: STATIC X STATICRENEWAL ______
TESTSUBSTANCE:
UcV ^ Vs)_________
DILUTIONWATER: TESTFISHSOURCE:
CARBON-FILTEREDWELLWATER(3MWELL2,ST. PAUL, MN)_________
TEMP.
pH 8 * 4 , D.O. 8 , 1mg/L
t <i c ,
Tqc^ CcAV, <vs . cCs_________DATERECEIVED:
FISHAGE:
d&NjS AVG.WT.: Q.QQA .0 3 g
AVG.SIZE: S.\ l>cm
NO.OFFISH/TESTCHAMBER: ______V_Q________
LOADINGRATE:
* / l V_* oVr\ .
TESTSOLUTIONVOLUME:
I L _________
SIZEOFTESTVESSELS:
^L
DATEINITIATED: ^-5.\~ci4 (& tUQQ DATETERMINATED:
Qt W-Oq^
TEST INITIAL TOTAL
VESSEL CONC. AMOUNT 24-HR mg/L ADDED*
BK1 CONTROL BK2 CONTROL
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MORTALrrY
D.O. mg/L
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PH
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D.O. mg/L
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D.O. p mg/L
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SECONDARY EFFECTS: S a Surfacing; E a Loss of Equilibrium; Q a Quiescent; R a Erratic Swimming; H a Hemorrhaging
ANALYST:
rr~R~D_________
001529
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Sample Description
Lab Request No. r A l Q V ^ i
Environmental Lab -- Work Sheet
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001530
4 B U R L I N G T O N RESEARCH. INC. TRIMMED SPEARMAN-KARBER METHOD FOR CALCULATION OF
EC50 AND LC50 VALUES IN BI0A3SAYS
FOR REFERENCE. CITE M.A. HAMILTON. R.C. RUSSO. AND. R.V. THURSTON, 1977. TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS. ENVIRON. SCI. TEGHNOL. 11(7) 714-71? CORRECTION 12(4) 417 (19785.
DATE
/21/94
"c.37 # __
___ M 1 C 1 8
CHEMICAL
___ F C -94 - X
SPECIES _
___ P. pramslas
DURATION
96 hours
RAW DATA
C G N C E N T R A T IO N <t r -.a / L )
3.20
5 . 6 0 10.00 IB. 00
NUMBER EXPOSED
20 20 20 20
MORTALITIES
' 0 X *7* 10
SPEARMAN-KARBER TRIM
0.00
SPEARMAN-KARBER ESTIMATES
LCSO
19.4886234
957. L O W E R C O N F I D E N C E
15.83
95% UPPER CONFIDENCE
24.00
*32 a O 0 13
nriioiS
56.00 20 20
3EST COPY AVAILABLE
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BURLINGTON RESEARCH. INC. T r i m m e d S o e a r m a n - K a r b e r M e t h o d -for C a l c u l a t i o n o-f
EC50 and LC50 Values in Bioassavs
X
X
X
3 l . .7
'- 3 T 2 . 0 1 2E
3 -3
r 3X
I3
3 1 .50? ;\i 3
3 X 3 1 .0 0 6 E 3
x ''
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QDUDDDEDDDDDDDDEDUDUDDUDUDDDDDEDPVDDDDDDDDDDDEDDDDDDDDDDDDDDE
LC10
LC25
LC50
^ LC75
LC100
MORTALITIES
001532
* * 3 A T E ______ ;_________________ 3 / 2 1 / 9 4
T E S T # __________________ " M 1018
' C H E M I C A L ___________________ F C -- 94-X
S P E C I E S ____________________ P. a r m e l a s
D U R A T I O N ____________ 72 hours
RAN DATA
uz
C O N C E N T R A T I O N (m a / L )
3.20
5.60
10.00
18.00
NUMBER EXPOSED
20
20 .
20
20
MORTALITIES
000 2
3PEARMAN-KARBER TRIM
40.00
SPEARMAM-KARBER ESTIMATES
LC50
35.5052032
' 957. L O W E R C O N F I D E N C E
14.86
957. U P P E R C O N F I D E N C E
S4.S5
32.00 20 10
^ 10 18
56.00 20 12
BEST COPY AVAILABLE
001533
'YllOl
6 .907
I 3 6.044E 3 7
5 . 1S0 3 3 X
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4 % BEST COPY AVAILABLE
BURLINGTON RESEARCH. INC. T r i m m e d S o e a r m a n - K a r b e r M e t h o d -for C a l c u l a t i o n o-f
EC50 and LC50 Values in Bioassavs
T
o 2.590E N3
3 3 3 1 . 726 3 3 3 3 .8634E
3
%'050EDDDUDDEDDDDDDDDDDDEDDDDDDDDDDDDDDEDDDUDDDDDDDDDDE
LC10
LC25
LC50
LC75
LC100
MORTALITIES
001534
