Document 4abbrzrkRVoBZkD0vB8MzKdGQ

AR fi.S.6 - 0 *\o2> ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE___________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3041. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 2300. The test sample is FC3041 (cc 809-1). Current information indicates it is a mixture of 6% PFOS, 36% diethylene glycol butyl ether, 36.05% water, 12% Sultone fbamer, 9% sodium octyl sulfate, 0.55% sodium lauryl sulfate, and 0.4% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f Hie test sample. METHOD:_______________________________ ____________ _________ Method: Not given Type: Static acute GUP: No Year completed: 1981 Species: Daphnia magna Supplier: Rand McNeilly Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: LC50 values calculated using the moving average angle method. Test conditions: Dilution water: Carbon-filtered well water, St. Paul, MN Dilution water chemistry: Hardness: 253 mg/L as CaC0 3 Alkalinity: 221 mg/L as CaC0 3 Lighting: Not given. Stock and test solution preparation: A 5000 mg/L primary stock solution was prepared by diluting 500 mg of test substance in 100 mL of dilution water. Aliquots were then added directly to dilution water in the test vessels. Exposure vessels: 250 mL beakers containing 200 mL exposure solution and a solution depth of 6 cm. Number of replicates: 2 Number of daphnids per replicate: 20 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 8.0 - 8.1 (control exposure) 8.0 -8 .1 (1000 mg/L exposure) 002902 Temperature range (0-48 hours): 18 - 20 C Dissolved oxygen range (0-48 hours): 7.9 - 9.3 mg/L (control exposure) 4.0 - 9.3 mg/L (1000 mg/L exposure) R E S U L T S __________________________________________ Nominal concentrations: Blank control, 65,100,155, 240, and 370 mg/L. Element values: 48-hour LC50 = 259 (242 - 276) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. - CONCLUSIONS______________________________________________ The test substance 48-hour LC50 was determined to be 259 mg/L with a 95% confidence interval of 242 to 276 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ R eliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S __________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6508S, 1981. OTHER_____________________________________________________ Last changed: 6/26/00. 002903 ENVIRONMENTAL LAEORTORY AQUATIC TWICITf DATA SHEET ,y *jC 6 *S O a S ^ rn * T ,,t*fa ,c /k ia s jS rta f**" '*1 T* , t * * F C iV ^ Q g ^ .^ ^ 6 0 -------------- Test --iK P U h l ftffff*****^^ I0 4 rk ip jt.i^ t* e#;,i |Jh)fiyet<* Mo- A ft: F irs t-la s ta v y to if < i.S -I.*a ) Ns. Organisms Fr tre e ta s n t^ Q ____________ te s t Chamber: 2S0al glass to tte r T tst Solution V o l.: 200*1 Ttst Solution Depth: Oca. T ta. B t. to n w zJlllHi t< o>--ei.t: * ( k m a to n < s w v i < ; . i r n f l i i : __ ___ ^ t^ v u rr-A h ^ i 4 - j !(,.?> Q.la -d U ft W - f t ? ? Effects other than Mortality EC50- Tank No. Control Symptoms or Reactions 24-Hr. Total No. Affected 48 Hr. 72-Mr. 96-Hr. -- - J_______ 1:oMc.ltsV f f e < j lOft-TgPtfU. , >t l ,,Y tr r i= y '- d T -A A tteB ? VrfrrtMV --- r-- Inlfill l lfl. ^mV-l-U-i--&TLTt ----------------------- ------------ - BEST COPY AVAILABLl 002904 ( Tyro Toot___._________ _ BNVIRONMEVTAI LABORTORY KJUATIC TOXICITY DATA SHEET *S 8< A ttrtil Tiitid ____tffB S Q Q -------------------- Tost OfganirfTyp)^u^|^|yorc ^ A i D Mrklgu/ffint mc'4\jtyltfUt<:h Ho.^ - f } / Af: First-Instar yomg (S.S-l.Soa) Ho . Crgooisno For trottatoi (1___________ T w t Oituhor; asoOnul | Ul iu > hooter Toot Solution Voi}. : 1JO0OuMl Toot Solution Dtpth: *Acs Tino Storto d L S S B t-- F" f w t * t j& J tlJ Z " Silution Urtor a n k y tH M W* --I r tifa, / f f *C. O O .& S -tf*- pHJgJ) tovoitifotcr im ~ P o # X . v.C s q z .s < \ M T f c W p OTES? r A y to m r r - - A E h C J \ t 2 . 1 ., i l j U >.2.j 3 . t o j * f l U J -.ft m O Effects othor thon Mortality **50- Took No. Control Sysptoao or loootiono Total Mo. AlfoetoS a4-Hr. ___ ** Hr._______ Tamr.________-Hr._______ 1 OHUBCntSP f e g r r (! V l V . ' d iM R a '' ^rcxwBaffim: BEST COPY AVAILABLE itq m n . fl J 00290! TABLE 2 ENVIRONMENTAL LABORATORY BLDG. 2-3E ST. PAUL, MINNESOTA FIRST QUARTERLY REPORT, 1981 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING PARAMETER TOTAL HARDNESS* as mg/1 C a C 03 TOTAL "M" ALKALINITY* as mg/1 CaCOj ELECTRICAL SPECIFIC CONDUCTANCE* as umhos per cm2 at 25C TOTAL SOLIDS* as mg/1 pH** STANDARD PLATE COUNT*** per 1.0 ml JAN. 28 FEB. .26 MAR. 25 AVERAGE (+ S) 256 252 252 253 + 2 232 220 210 221 + 11 360 305 320 328 + 28 267 299 311. 292 + 23 7.7 7.7 7.7 7.7 0 19 6 11 12 + 7 CRITERIA OF CONSTANT QUALITY Monthly ranges should be less than 10% of their respective averages. (ASTM Standard E729-1980.) ** M onthly range of pH should be less than 0.4 unit. (ASTM Standard E729-1980.) *** Total bacterial densities should not be greater than 500 organisms per ml. (U S E P A - 6 0 0 / 8 - 7 8 - 0 1 7 , p. 101.) 002906