Document 4aZ87N5v0RRzdva6pQK9Krj7V

~a~-- 3I 1-k Company Sanitized . Does not contain TSCA CBI . [ ) : TRANSFORMATION POTENTIAL IN AEROBIC AND ANAEROBIC SOILS WILDLIFE INTERNATIONAL, LTD . PROJECT NO. : 112E-108 Organisation for Economic Cooperation and Development OECD Guideline 307 AUTHORS : Edward C. Schaefer Joel Stenzel Raymond L. Van Hoven STUDY INITIATION DATE: March 10, 2005 STUDY COMPLETION DATE: March 17, 2008 Submitted to: E.I . du Pont de Nemours and Company Wilmington, Delaware 19898 USA Wildlife International, Ltd. 8598 Commerce Drive Easton, Maryland 21601 USA 1 (410) 822-8600 Page 1 of 298 p. 2 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No . : 112E-108 -2GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT SPONSOR : E.I. du Pont de Nemours and Company TITLE: C ) Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROJECT NO. : 112E-108 STUDY COMPLETION DATE : March 17, 2008 This study was conducted in compliance with Good Laboratory Practice Standards as published by the U.S . Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MCICHEM (98) 17), with the following exceptions : Periodic analyses of well water for potential contaminants were performed using a certified laboratory and standard U.S . Environmental Protection Agency analytical methods. The characterization of the test substance, and the stability of the test substance under the conditions of storage at the test site, were not determined in accordance with Good Laboratory Practice Standards. Original documentation for the preparation of two combined stocks were not maintained . Documentation ofthe stocks preparation was re-created from the information on the stock label. STUDY DIRECTOR : Edward C. Schaefer U Director of Biodegradation I 7 P"'142c if pop ' . _ DATE p. 3 Company Sanitized. Does not contain TSCA CBI . Wirdl'ife International, Ltd, Project No . : 112E-108 -3- QUALITY ASSURANCE STATEMENT This study was examined for compliance with Good Laboratory Practice Standards as published by the U.S . Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MC/CHEM (98) 17). The dates of all inspections and audits and the dates that any findings were reported to the Study Director and Laboratory Management were as follows : ACTIVITY : DATE CONDUCTED: DATE REPORTED TO : STUDY DIRECTOR : MANAGEMENT : Protocol March 1, 2005 March 1, 2005 March 11, 2005 Stock Solution Preparation IIeadspace Sampling Stock Solution Preparation Analytical Data-Day 0 Analyses Analytical Data -- Week 1 Analyses Analytical Data -- Week 2 Analyses Analytical Data -Month 1 Analyses Analytical Data - Month 2 Analyses Analytical Data-Month 4 Analyses Soil Moisture Determination and Dosing Data Oxygen Measurement Dosing Viability Controls Microbial Biomas.4 Sample . Processing . March 15, 2005 March 24, 2005 April 19, 2005 October 10-26, 2005 October 10-28, 2005 October 10- November 1, 2005 October 10- November 1, 2005 October 10- November 1, 2005 October 10- November 2, 2005 October 10- December 2, 2005 October 17, 2005 October 17, 2005 October 18, 2005 March 15, 2005 March 24, 2005 April 19, 2005 October 26, 2005 October 28, 2005 November 1, 2005 November 1, 2005 November 1, 2005 November 2, 2005 December 2, 2005 October 17, 2005 October 17, 2005 October 18, 2005 March 16, 2005 April 13, 2005 April 22, 2005 . November 8, 2005 November 21, 2005 November 21, 2005 November 21, 2005 November 21, 2005 December 1, 2005 August 30, 2006 October 19, 2005 September 26, 2006 November 3, 2006 Company Sanitized . Does not contain TSCA CBI . Wirdife International, Ltd. Project No.: 112E-108 ACTIVITY: Analytical Sample Processing (6-month) Month 6 Analytical Data Analytical Draft Report 9 and 12 Month Analytical Data and Tables Biological Data and Draft Report Data and Draft Report (including 15,18 and 24 month analytical data) hinal Report 4 QUALITY ASSURANCE STATEMENT (continued) DATE CONDUCTED: DATE REPORTED TO : STUDY DIRECTOR : MANAGEMENT : October 20, 2005 November 1417,.2005 November 17-22,2005 August 21-25 and 28, 2006 October 23 November 9, 2006 October 24 November 2, 2007 October 20, 2005 November 17, 2005 November 22, 2005 August 29, 2006 November 9, 2006 November 2, 2007 October 24, 2005 November 28, 2005 December 1, 2005 October 3, 2006 December 18, 2006 November 20, 2007 March 17, 2008 March 17, 2008 March 17, 2008 All inspections were study-based unless otherwise noted . Y~ . KAo6.r Linda R. Mitchell Director of Regulatory and Ecotox Operations /7 Al"c.v .2b0$ DATE Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No . : 112E-108 -5REPORT APPROVAL SPONSOR : E.l. du Pont de Nemours and Company TITLE: C ) Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROJECT NO.: 112E-108 STUDY DIRECTQR : PZI in Edward C. Schavier Director of Biodegradation 17 4+-4,C. cef 2o ok DATE a,rc(, t'l.0. r) 19 DATE MANAGEMENT : rJ Henry O. Krueger, Ph.D. Director of Aquatic Toxicology/Terrestrial Plants and Insects 17 DATE Willard B. Nixon, Ph, Director of Chemistry ,10or DATE Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -6- TABLE OF CONTENTS Title Page . ................ .. ... ... .... ..... .. . .. .. .. ....... ..........., ...... .. ..... .. ..... .. .. ... .. .. ... .. .. ..... .. .. .. ......... .. ..... .. ....... .. ..... .. ..... .1 Good Laboratory Practice Compliance Statement .. .... ... .. .. ... .. . . . .. .. .. ..... .. ....... .... ....... .. .. .. . .. ..... .. .. .. . . ... .. . .. .. .. . .2 Quality Assurance Statement ..... ......... .. .... ... .. .. .. ... .... .. ... .. .. ..... .. . .... .. ..... .. .. ..... .... .. .. ... .. .. .. . . . .. .. . .. .... .. ... .. .. ..... ..3 Report Approval . . ........ .. .. ... .. ..... .. ... .. .. .. . .. .. .. .. ... .. .. .... . .. .. ....... ......... ....... ......... .. .. . .. .. .. .. ... .. .. ... .. .. ..... ..... .. .. .......5 Table of Contents .. ..... ....... ... .. .. . .. .. .. ... .. .. ......... .. ....... .. ..... .. .. ... .. .. ... .. .. ..... .. ... .. .. .. .. . .. .... ......... ..... ........ . ... .. .......6 Study Information.... .. . ....... ... .... ... .. .. ......... .. ..... .. .. .. .. . .. .. ... .. .. ... .. .. .. . . ... ..... .. .. ..... .. .. ........... ....... ....... .. .. .......... .16 Introduction ...... .. ..... .. .. ... .......... ................ .. .. .... . .. .. .. .. . .. .. .. . .. .. .. . .... .. ... .. ... .... .. ......... ........... .. ... .. .. .... . .... . .. .. .. . .17 Objective ... .. .. .. . .. .. ... . . .. ... .. .. ... .. ..... .. .. ... .. .. .. . .. .. .. .. ... .... ..... .. ..... .. ....... ....... ....... .. .... ..... .. .. .. ..... .. ....... .. ..... .. ..... ..17 Experimental Design ... .. ... .. .. . .. .. ... .. .. .. ..... .. .. ......... ......... ................... ..... .... ....... .. .. .. .. ... .. .. ..... ......... ....... ... ....17 Materials and Methods .. .. ..... .. ... .. ... .. .. .. ... .. .. .. ......... .. ..... .. ..... .. ..... .. ..... .. ....... .. ....... .. .. ......................... ..........18 Test Substance...... . .. .. ..... .. ... .. .. ... .. .. ... .... .. .. .. ..... .. ....... ..... .. ....... ....... .... ... .. ............................. .. ..... ....... ...18 Measured Analytes ........... ......... .. ... .. .. ...... . .. .. ... . . .... .. . .. ....... .. .. . .. .. .. ....... .................. ....... ....... .. ..... .. ..... .. .18 Internal Standards.......... ... .. ....... .. ....... ....... .. .. .. . . . .. .. .. ..... ... .. .. .. ... .... . .. .. .. .................. ....... ......... .... ... .. ... .. .21 Test Soils.. .. ....... .. ... .. ..... ... .. ....... .. ..... .. .. .. .. ..... .. .. ..... .. ... .. .. ..... ..... .... ... .. .. ......... ......... .. ..... .. .. ....... .. ... .. ... .. .22 Test Apparatus and Conditions . .. .... ... .. .. .. ....... .. ....... ..... .. ....... ....... ....... ........... .. .... ..... .. . .... ....... .. ..... .. .. ..23 Soil Pre-Incubation . .. .. ..... .. ... ......... .. ....... .... ..... .. ....... ..... .. ....... ......................... .. .. .. ..... ..... .... ......... ..... .. ..23 Preparation of the Test Chambers . .. .. .. ..... .. .............................................. .... ... .. .. .. .. ............ ......... ..... .. ..23 Maintenance of Test Chambers .. ....... .. ... .... .... ....... ....... .. ..... .............. .. ..... .. .. .. .. ... ............... ....... .. ..... ... ..24 Sampling and Measurements ......................... .. ..... .. ..... .. .. ... .. .. ... .. .. ....... ........... ........... .. ..... ....... .. ..... .....25 Quality Criteria ........ .. ..... ..... .. ....... ........... .. ..... .. .. .. .. . .. ... .. .. ..... .. ..... .. .... ... .. .. ......... ......... .. .... . .. .. ... . . ..... .....25 Viability Assessments ......... .. ........... ....... .. ..... .. .. .. .. . .. .. .. . .. .. . .. .. .. ....... ..... .. .. .... ..... .. ....... .. ..... .. .. .. .. ... .. ... .. .26 Results ..... ....... .. .. .. ... .. ... .. ..... ..... ....... .. .. .. ... .... .. ..... .. ....... ....... ....... .. .............. .. ...... . .. .. .. .. ... .. .. . .. .. .. ... .. .. ... .. ..... .26 Test Conditions ...... .. ....... ..... ..... .. .. .. ... .... .. ....... .............. .. ..... ........... ....... .. ..... .. .. .. .. ... .. .. ... .... ..... ..... .... ....26 Characterization of Soils . .. ... .. ... .... .. ................ .............. .. ......... .. ..... .. ..... .. ......... .. .. ..... .. ....... .............. ....27 Sample Processing and Analyses .. .. .. ............. ..... .. .. ... ..... ......... ....... .... .. . .. .. .... ... .. .. ..... .. ....... ................ ..27 Headspace Analyses ... ....... .. ....... .... .. ..... .. ..... .. .. ... .. .. ... .. ..... .. .. .. . .. .. .. . .... .. .. ......................... ..... .........27 Soil Analyses . ..... ............................ .. .. .. .. ... ... .. .. ....... ..... .. .. ... .. .. .. ..... .. ... ........ ....... .. ..... .. ..... .. .. ... .. .. ...27 Stock and Calibration Standard Preparation .. ..... .. ... .. ... .. .. ..... .. .. ..... .. .. ................ .. .. ..., . .. .29 Calibration Curves and Limits of Quantitation ... .. . .. .. .. ..... .. ... .. .. ....... ........... .. .., . . ., .,... ,31 References . .. ....... .......... .. .. ... .. .. . .. .. . . ..... .. ....... ........... .. ..... .. ..... ... .... .............. .. .. .. ....... .. ..................... ....... ......34 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -7TABLE OF CONTENTS - Continued - TABLES Table 1 . Frequency of Measurements . .. .. .. ... .. .. .. ..... ......... ... .. .. ..... .. ... .. .. . ....... .. .. .. ........................... .... . .....35 Table 2. Test Chamber Headspace Oxygen Content.............. ..... ....... .. . .. .. .. ..... .. ........... ....... .. .. .... . .. .. ... .. .36 Table 3. Table 4. Selected Soil Properties .. ......................... .. .. ... .. ..... ....... ..... ............ ........... .. ....... .... ................... .37 Soil Viability by Glucose Mineralization :Alfisol.. .. .... . .. .. ... .. ..... ....... .. .. .. ... .. .... ..... .. .. ......... ... ....38 Table 5 . Soil Viability by Glucose Mineralization :Inceptisol ... ... .. ... .. ........... . .. .. .. ......... ....... ...., . ..... ..... .,38 Table 6. Soil Viability by Glucose Mineralization :Ultisol ... ....... ..... .. ... ... .. .. .. .. ......... ......... ...... . .... ., ... .. ., .39 Table 7. Soil Viability by Glucose Mineralization :Mollisol .... .. ..... ... ..... .. ....... .... .. .. ..... .. ..... .. .. .. .. ... .. ... .. .39 Table 8. Soil Microbial Biomass . ... .. .. ... .. .... . .. .. .. .. ... .. .. ... ... .. .. .. .. . .. .. . .. ..... ....... . . .. .... ... .. .. ... .. .. .............. ... ...40 Table 9. Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas.. .. ..... .. 42 Table 10. Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts..... .. ....... ....... . .. .. .. .. ......... .. .. ....,.. ...,..,43 Table 11 . Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts ..... .. .44 Table 12. Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil Extracts .... ... ....... ..... ..... .. ..... .. ..... .. .. ......... .. ..... ..... ..... .. ..... .. ... .. ... .. ..... .. .. .. ........... ....... ........ . .. ... .. ..45 FIGURES Figure 1 . Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers .... ......46 Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts . .......... .. ... .... .... ....... .. ..... .. .. .. .. .., .. ,47 Figure 3. Representative ion chromatogram for 8-2 FTOH in a low-level (25.0 ng a.i ./mL) calibration standard by GC/MS . ..... . . . .... .. ..... .. ... ..... .. .... . .. ... .. ..... .. ..... .. .. .. .. ... ........................... ...48 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -8TABLE OF CONTENTS - Continued - Figure 4. Representative ion chromatogram for 8-2 FTOH in a high-level (500 ng a.i ./mL) calibration standard by GC/MS . .... . .... .. .......... .. .. .. ......... ....... .. .. ... .. .. .. . .. .. .. ... .. ... .. .. ....... .. .. ..........49 Figure 5 . Representative ion chromatogram for 8-2 FTOH in a low-level (10.0 ng a.i ./mL) calibration standard by LC/MS/MS ........ .. ..... ...... ... .. .. .. . .. .. ... .. .. .. .. ..... ..... .. .. ... .. ... .. .. .. ... .. .. .... .. .. ..50 Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i ./mL) calibration standard by LC/MS/MS .. . .. .. .. ... .. .. . ...... .. ..... .. .. ... .. .................. ..... ..... .. ....... .. ....... . . .. ..51 Figure 7. Representative ion chromatogram for 7-2 sFTOH in a low-level (5 .00 ng a.i ./mL) calibration standard by LC/MS/MS .. .. ..... .. .............. ........... .. ..... .. .. ... .. .. .. . .. .. ... .. .. ... .. .. .. ..... .. .. .....52 Figure 8. Representative ion chromatogram for 7-2 sFTOH in a high-level (250 ng a.i ./mL) calibration standard by LC/MS/MS . ....... ....... .. .. .. . .. .. .. .. . .. .. .. ....... .. ..... ... .. .. ..... .. ... .. .. ........... .. .. .. ..53 Figure 9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS . .. ... ........ ................ .. ..... .. ..... ..... .. ....... .. ......... .. .. .....54 Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS .. ..... .. ..... .. .. .. .. ....... .............. ..... .. ....... ..... .............. ..55 Figure 11. Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. . .. .. .. .. ....... ........... .............. .............. ....... .... .. .. ..... ....56 Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS... ........... .. .. ... .. .. ....... .. ....... .. ... .. ..... .. ....... ......... .... .. .. .57 Figure 13 . Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . .. .. ......... ....... .. ... ....... ....... ... ..,.58 Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5 .00 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS ......... .... .. . .. .. .. . .. .. .. . ....... .. ..... .. ..... . . .. .... .. ..59 Figure 15 . Representative ion chromatogram for PFOA in a low-level (0 .100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS ...... .. .. ..... .. ..... .. ..... .. ..... .. ..... .. ........... .. .. ... 60 Figure 16. Representative ion chromatogram for PFOA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS ....... ......... .. ... .. .. ... .. ..... .. ..... .. .. ......... .. ......61 Figure 17 . Representative ion chromatogram for PFNA in a low-level (0 .100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS .... .. .. ....... .............. ..... .. ....... ........... .. .. .. . .. 62 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -9TABLE OF CONTENTS - Continued - Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS ... .. ..... .. .................. ..... ..... ....... .... ....... .. ...63 Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . .. ....... .. ..... ...... .. ..... ............ ..... .......... . .. .. .64 Figure 20. Representative ion chromatogram for PFDA in a high-level (5 .00 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . .. .. ..... ............. ....... ............ ..... ............... .65 Figure 21 . Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by GC/MS ............................ .. ..... .. ..... .............. .. .. .. ..... .66 Figure 22. Representative ion chromatogram for PFUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS ..... ................... ........... .... . .. .... . .. ..... .. .. ... .. ....... .. .. ..... .67 Figure 23. Representative calibration curve for 8-2 FTOH by GC/MS ...... .. .... .. . .... ... ..... .. .. ... .. ....... .. .. .. ....68 Figure 24 . Representative calibration curve for 8-2 FTOH by LC/MS/MS . ...... .. .. .. . .. .. .. . .. .. ... .. ....... .. .... ....69 Figure 25 . Representative calibration curve for 7-2 sFTOH by LC/MS/MS ...... .. .... . .. .. ... .. .............. .. .. .. ....70 Figure 26 . Representative calibration curve for 8-2 FTA by LC/MS/MS ... ...... ... .... ... .. ... .. ..... .. .. .. ... .. .. .. ....71 Figure 27 . Representative calibration curve for 7-3 Acid by LC/MS/MS . ..... .. .. ..... .. ... .. ... ..... .. .. .... ... .. .. .. ..72 Figure 28 . Representative calibration curve for 8-2 FTUA by LC/MS/MS .. .. .. .. ..... ..... .. ... .. ..... .. .... . ...... ....73 Figure 29 . Representative calibration curve for PFOA by LC/MS/MS . .. .. ......... ....... ....... ..... ... .. .. .. ......... ..74 Figure 30 . Representative calibration curve for PFNA by LC/MS/MS .. .. .. ......... ....... ....... ..... .. ... .. .. ...........75 Figure 31 . Representative calibration curve for PFDA by LC/MS/MS ... .. .. ............................ ....... .. .... .....76 Figure 32 . Representative calibration curve for PFUA by LC/MS/MS .... .. .. ........... ....... ........ .. ......... .... .. ...77 Figure 33 . Representative ion chromatogram for 8-2 FTOH in the sampled headspace (Cig cartridge extract) from a Day 0 untreated control soil by GC/MS ..... ........... ....... .. ..... .. ..... ....... .. ..... .. .....78 Figure 34 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS .... .. ..... ..... ....... ....... ......... ..... .. .. .... . .. .. ... .. ................... .. .. .. .79 p. 10 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -10TABLE OF CONTENTS - Continued - Figure 35 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS ..... .. .. .. . .. .. .. .. ..... .. ....... . .... .. ..... .. ..... .. ... .. .. ........... .. .. ... .. .. .. ..80 Figure 36 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 untreated control soil by LC/MS/MS .. ... . . .. .. .. ......... .. ... .. ..... .... ............ .. ..... ......... .. .. .. . .. .... . . .......81 Figure 37 . Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS .. .. .. ..... .. .. .. .. .. ... ....... .. .. ... .. .. .. . .. .. ... ....... .. .. .. . .. .. .. .. .... ....... .. .82 Figure 38 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS .......... .. .. .... ..... .. ... .. .. ....... ............ .. ... .. .. ..... .. .... ....... .. ........83 Figure 39 . Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS .. .. . .. .... . . . .. .. . .. .... .. ................................ ....... .............. .. .. .. . .. .. .... .... ..... 84 Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS .... .. ... .. .. ....... ........... .. .. ..... .. ... .. .. ....... .. ... .... . .. .. ... .. .. .. .. ....... ...... ..... .. .. .85 Figure 41 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS ... ......... ..... .. ..... .. .. .... . .... .. ....... ..... .. .... ... ... .. .. ... .. .. ........... .. ..... . . .. .. .. . ...86 Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS .. . .. .. ... . . .. .. .. . .. .. .. ........... ....... ....... .. .. ... .. ..... ....... .. ..... .. .. .. .. . .. .. .. ...........87 Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,$ cartridge extract) from a Month 24 untreated control soil by GC/MS ... ... .. .. .. . .. .. ... .. ..... .. .. .. .. .................. 88 Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS ...... .. .. .. .. ....... ... .. .. ..... .. ..... .. .. .. ........ .. ..... .. ............. .. ..... .. .. .89 Figure 45 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS . .. .. .. ......... .... ..... ....... ...... . .... ... .. ..... ....... .. ..... ........ ....... ......90 Figure 46. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS .... .. ....... .. .. .. ..... .. ..... .. ..... .. ..... .. ..... .. . .... .. ..... ........ ..... .... ... ..91 Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS . .. .. ....... ........... .............. ....... .. ..... .............. .. .. .. .. ....... ..... .. .92 Figure 48 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS ......... .. .. .. ..... .. .. ... .. .. ..... .. . .. .. .. ..... .. . .. .. ......... ........... .. ..... ....93 p. 11 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -11TABLE OF CONTENTS - Continued - Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . .. .............. ..... .. . .. ... .. .... ........ .. .. ... . . .. .. .................... ....... ... .94 Figure 50 . Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . ...... ............ ..... ..... .. ... .. ... .. .. ..... .. .. ....... .... .. ..... .. ..... .. .. ... .. ..95 Figure 51 . Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS ....... .. .. ..... ..... .......... .. ..... ....... ........... ......... .. ..... .. .. .. . .. .... . ..96 Figure 52 . Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS .. .. .. .. ....... ... ..... .. ... ..................... .. ..... .... .. .. ..... .. ....... ....... ...97 Figure 53 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Day 0 fortified (250 [tg 8-2 FTOH/kg and 10 .0 pg PFCAs/kg) control soil by GC/MS .... ..... .. .. ... .. .... .. ........... ..... ..... ....... . .. ... .... .. ....... ....... .. ........... .. ..... .. ..... .. ... .98 Figure 54 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 ltg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. . .. .. ... .. .. ..99 Figure 55. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 itg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS . .. ... .. .. . .. 100 Figure 56 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 ~tg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS ..... ....... . 101 Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 Itg 8-2 FTOH/kg and 10.0 ~tg PFCAs/kg) control soil by LC/MS/MS ....... ...... 102 Figure 58. Representative ion chromatogram for PFNA in the -soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS.. .. ... .... .. 103 Figure 59 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 ~tg 8-2 FTOH/kg and 10.0 Ftg PFCAs/kg) control soil by LC/MS/MS. ............ 104 Figure 60 . Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS ............. 105 Figure 61 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Month 12 fortified (250 ug 8-2 FTOH/kg and 10 .0 pg PFCAs/kg) control soil by GC/MS... .. ..... .. ..... .... ..... ............. ... .. ... .. .. . ....... .............. .. .. ..... .. .. .. ..... .. ....... ... .. ..106 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -12TABLE OF CONTENTS - Continued - Figure 62 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 llg PFCAs/kg) control soil by LC/MS/MS. . . ... .. .. ... 107 Figure 63 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 ltg PFCAs/kg) control soil by LC/MS/MS . ............ 108 Figure 64 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS .... ... .. .. .. 109 Figure 65 . Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (25011g 8-2 FTOH/kg and 10.0 llg PFCAs/kg) control soil by LC/MS/MS.. .... .. ..... 110 Figure 66 . Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 jig 8-2 FTOH/kg and 10.0 ltg PFCAs/kg) control soil by LC/MS/MS. ............ Ill Figure 67. Representative ion chromatogram for PFDA in the soil extract from a Month 12 fortified (25011g 8-2 FTOH/kg and 10.0 llg PFCAs/kg) control soil by LC/MS/MS ..... .. .. .... 112 Figure 68. Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS.. .... ....... 113 Figure 69 Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Day 0 sterile soil treated with 200 mg/kg H-26634 by GC/MS . ....... .............. 114 Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kg H-26634 by LC/MS/MS ..... ........... .. ..... .. .. .. .. . .. ... .. .. .. . .. ...,., ... . ,115 Figure 71 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg (_ ]by LC/MS/MS .. .. .. ... .. .. .. .. ... .. .. ..... .. ... .. .. ... .. .. . ., . 116 Figure 72 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kC ]by LC/MS/MS . .. ... .. .. ........... .. ..... .. ..... ......... ..... ......... .. .117 Figure 73. Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg~ )by LC/MS/MS ..... .......... ..... .. ... .. .. ... .. .. ... .. 118 Figure 74 . Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg( ) by LC/MS/MS .... .. .. .................... .......... ....... ......... .......119 Figure, 75 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kgC' _Iby LC/MS/MS ............ .. .. .. .. ..... .. ..... ..... .. ... .. ,120 Company Sanitized . Does not contain TSCA CBI . Wildlrfe International, Ltd. -13TABLE OF CONTENTS - Continued - Project No.: 112E-108 Figure 76. Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg (_ ] by LC/MS/MS ....... ......... .......... ......... ....... .. ....... .. .... .. ..121 Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Month 12 sterile soil treated with 200 mg/kgL _-~y GUMS...... .. .... .... 122 Figure 78 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kg C ,)by LC/MS/MS .. .. ..... .. ..... ............................ ........... .. .. ..123 Figure 79 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kK '.) by LC/MS/MS .... ..... .. ..... ....... ....... .............. .................124 Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgG ' by LC/MS/MS .. .. ..... .. .. ..... ..... .. ..... ....... .... ..... .. .. .. .........125 Figure 81 . Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg[ .1by LC/MS/MS .... ..... .... .. . .. ..... .. ..... ....... ......... .. .. .. .... .....126 Figure 82. Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg` :) by LC/MS/MS . .. ..... ..... .. .. . .. .. .. . .. .. ... .. ... .. .. ..... .. ........ .....127 Figure 83 . Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgL )by LC/MS/MS . .. ..... .. ....... ..... ..... .. .. . .. ....... .. .. ........... .. ...128 Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg C 3by LC/MS/MS ................. ..... .. ....... ....... ........... ....... .. .. .129 Figure 85. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8 cartridge extract) from a Day O live soil treated with 200 mg/kg ~ by GC/MS .. ......... .. .... .. .. ....130 Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day O live soil treated with 200 mg/kgC 3by LC/MS/MS .. ..... .. ..... .. ..... ..... ..... ....... ......... .. .. .. .. .. ....131 Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day O live soil treated with 200 mg/kgC ,)by LC/MS/MS .. .. ... .. .. .. . .. ..... .. ..... .. ..... ....... .. ..... ........ ....132 Figure 88. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day O live soil treated with 200 mg/kgL 1by LC/MS/MS . ... .. .. . .. .. .. ... .. .. . .. ... .. ..... .. .. ... .. .. .... ......... ..133 Figure 89 . Representative ion chromatogram for 7-3 Acid in the soil extract from a Day O live soil treated with 200 mg/kg ( )by LC/MS/MS . ..... .. ....... ..... .. ... .. .. ... .. .. . .. .. ... .. .. .. ......... ..134 Company Sanitized . Does not contain TSCA CBI, Wildlife InteYnational, Ltd. Project No.; 112E-108 -14TABLE OF CONTENTS - Continued - Figure 90 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day O live soil treated with 200 mg/kg L ~~by LC/MS/MS .. . .....,.,. ... .,.,. .., .. ... ,...,.. 135 Figure 91. Representative ion chromatogram for PFOA in the soil extract from a Day 0 live soil treated with 200 mg/kg C )by LC/MS/MS ..,. ..,.. .. .....,.. ., .. ... .. .. .,.. .,. 136 Figure 92 . Representative ion chromatogram for PFNA in the soil extract from a Day O live soil treated with 200 mg/kg[ ,by LC/MS/MS . .. .. ... .. .. ... .. ... ....... ....... .. .. .. .. ... .. .. ........ ... .137 Figure 93 . Representative ion chromatogram for PFDA in the soil extract from a Day O live soil treated with 200 mg/kgt -3 by LC/MS/MS .... ...,....,...... .. ..... ...., ., ...,..,....,.., .. ,138 Figure 94. Representative ion chromatogram for PFUA in the soil extract from a Day 0 live soil treated with 200 mg/kgt 73by LC/MS/MS . .. ... .. ..... .. ... .............., . ,139 Figure 95 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C18 cartridge extract) from a Month 24 live soil treated with 200 mg/kg(* 3oy GC/MS ...... .... . .... .. 140 Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg C I by LC/MS/MS . .. . .. .. . .. .. .. . .. ..... .. ..... ....... .. .. .. .. ........... .. ...141 Figure 97 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg .t 1by LC/MS/MS .......... ....... ... .. ., .., ., .. . .,....,. .,.,. 142 Figure 98 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 mg/kg[- - ly LC/MS/MS .... .. .. . .. ..... ..... .......... .... .. .. ...................... 143 Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live soil treated with 200 mg/kg :C ~~y LC/MS/MS .................... ..,.... .. ... .... .........,. .. .. .. .. ... .. .144 Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 mg/kgC ) by LC/MS/MS . ... .. ... .. ..... ................ .. ......... .. .. .. ....... .. 145 Figure 101 . Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil treated with 200 mg/kg( )by LC/MS/MS .. ............... ... .. .. ..... .. ........... .. .. .. .. ....... . 146 Figure 102. Representative ion chromatogram for PFNA in the soil extract from a Month 24 live soil treated with 200 mg/kgc )by LC/MS/MS . ..... .. ..,.. .. . .. .. . .. .. ..... .. .. .. ....... .. .. .. .. .. ... .. 147 Figure 103. Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kg[ jDy LC/MS/MS .. ..... ......,.,.......,. ..,.,.,.. 148 p. 15 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -15- TABLE OF CONTENTS - Continued - Figure 104 . Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil treated with 200 mg/kg~ _1by LC/MS/MS ........... ... ......... .. .... . ......... ...:... .... .. .. ....149 APPENDICES Appendix I. Measured Concentration of Selected Analytes (pg/kg Soil Dry Weight) .... ....... .... ........150 Appendix II . Measured Concentration of Selected Analytes (pmol/kg Soil Dry Weight) .. .. .. ..... ........223 Appendix III. Example Calculations . .. .. .. . .. .. ... .. ..... .. .. .. ... .. .. . .. .. .. ..... .. ... .. .. . .. ... .. .. ..... .. ..... .. .. ..... .. .. ...... .....296 Appendix IV. Duration of Incubation . .. .. . .. .. .. . .. .. . .. .. .. .. ..... .. ..... .. ..... .. ..... .. . .. .. . .... ..... .. .. .. . .. ... .. .... .... .. .......298 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -16- STUDY INFORMATION Study Initiation Date : Experimental Start Date : Experimental Termination Date : Study Completion Date : 10 March 2005 11 March 2005 07 September 2007 17 March 2008 Project No. : 112E-108 Study Director: Sponsor : Sponsor Representatives: Study Personnel: Edward C. Schaefer E.I. du Pont de Nemours and Company William Berti and Bogdan Szostek Edward C. Schaefer, B.S ., Director of Biodegradation Raymond L. Van Hoven, Ph .D ., Scientist, Chemistry Joel I. Stenzel, B.S ., Senior Chemist R. Scott Flaggs, B.S ., Biologist, Biodegradation Abul Siddiqui, B.S ., Scientist, Biodegradation Frank Lezotte, B.S ., Senior Chemist, Chemistry David L. Weiss, M.S ., Chemist, Chemistry William Schutt, B.S ., Chemist, Chemistry John R. Murrell, B.S., Senior Chemist Willard B. Nixon, Ph .D ., Director of Chemistry Henry O. Krueger, Ph.D ., Director of Aquatic Toxicology/Terrestrial Plants and Insects Molly E. Matthews, Biologist, Biodegradation Jon A. MacGregor, B.S., Scientist Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -17- INTRODUCTION This report describes a laboratory research study conducted to assess aerobic and anaerobic transformation ofC In soil systems. This study was conducted by Wildlife International, Ltd. for E.I . du Pont de Nemours and Company at the Wildlife International, Ltd. research facility in Easton, Maryland . The raw data generated by Wildlife International, Ltd. and the original final report final report will be archived by the study sponsor. A certified copy will be retained under Project Number 112E-108 in the archives located at on the Wildlife International, Ltd. site . OBJECTIVE The objective of the research study was to assess the potential for transformation of the test substance in aerobic and anaerobic soils. EXPERIMENTAL DESIGN The test was conducted with four test soils under aerobic conditions . The study was initially designed to set-up to also include four soils under anaerobic conditions, however a check of the system indicated that the test chamber closures did not exclude air. The systems initially started under anaerobic conditions up to and including month 6 are included in this report because they were extracted and analyzed as planned . Four groups were established for each soil type and condition (aerobic or anaerobic) : (1) untreated (control) live soil ; (2) 200 mg test substance/kg dw treated live soil ; (3) 200 mg test substance/kg dw treated sterile soil ; (4) 250 p.g 8-2 telomer B alcohol/kg dw treated sterile soil ; 10 pig 8-2 telomer B acid /kg dw ; 10 pg 8-2 unsaturated telomer B acid/kg dw ; and 10 p,g perfluorooctanoic acid/kg dw, heptadecafluorononaoic acid, nonadecafluorodecanoic acid, and perfluoroundecanoic acid at 10 jig/kg dw all added to sterile soil . The test systems reported here were incubated in sealed containers at approximately 20C for at approximately 24 months . Two incubation chambers from each of the groups were removed at appropriate time intervals and the soil samples were extracted and analyzed for potential metabolites . In addition to the day zero samples, eight samplings were performed with all of the groups . Three additional sampling intervals were performed with only the untreated control (1) and 200 mg test substance/kg dw treated live soil (2) groups . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -18- Additional untreated and test substance treated soil chambers were prepared for use as matrix fortification samples, biomass determinations, viability controls and to monitor aerobic conditions as necessary . " The test groups were prepared using 25 grams (dry weight equivalent) of soil. While this amount of soil is less than the 50 to 200 grams specified in the OECD 307 guideline, it was chosen to allow for 1) the extraction of the entire test chamber contents and internal surfaces ; 2) sufficient headspace to minimize the need for active aeration which could result in a loss of potential transformation products . METHODS AND MATERIALS The test system and study conditions are based on the OECD Guideline for Testing of Chemicals, Guideline 307, Aerobic and Anaerobic Transformation in Soil (1). As this guideline is designed for low molecular weight substances, but not for polymers, adaptations of the method may be necessary for feasibility reasons. Nevertheless, the study was carried out in such a way that the recommendations given in the guideline were followed as closely as possible . Test Substance The following substance was used in this study : ID: ~ Date receivedd: 2 November 2004 Wildlife International Ltd. ID: 6918 Measured Analytes Name: 8-2 Telomer B Acid, 8-2 Fluorotelomer Acid Synonym used in Report: 8-2 FTA CAS Name: Decanoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro-(8CI,9CI) CAS Number: 27854-31-5 Molecular Weight : 478.1 g/mole ID : H-26210 Date received: 1 September 2004 Expiration/reanalysis date : 12 October 2007 Purity : 97%; 3% 2H-Hexadecafluoro-2-Decanoic Acid Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd, Project No.: 112E-108 -19- Description : Solid Wildlife International Ltd . ID: 6826 Name: 8-2 Telomer B Alcohol, 8-2 Fluorotelomer Alcohol Synonym used in Report: 8-2 FTOH CAS Name: 1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro- (7CI, 8CI, 9CI) CAS Number: 678-39-7 Molecular Weight : 464.11 ghnole ID: H-24691 Date received : 1 September 2004 Expiration/reanalysis date : 12 October 2007 Purity: 99.2% Description : Solid Wildlife International Ltd . ID : 6828 Name : 8-2 Unsaturated Telomer B Acid, 8-2 Fluorotelomer Unsaturated Acid Synonym used in Report: 8-2 FTUA CAS Name: 2-Decenoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-hexadecafluoro- (9CI) CAS Number: 70887-84-2 Molecular Weight : 458.1 g/mole ID : H-26211 Date received : 1 September 2004 Expiration/reanalysis date : 12 October 2007 Purity : 98%; 0.5% 8-2 FTOH; 1 .5% unknown Description : Solid Wildlife International Ltd. ID: 6829 Name : N-Perfluorooctanoic Acid Synonym used in Report: PFOA CAS Name: Octanoic acid, pentadecafluoro- (8CI, 90) CAS Number: 335-67-1 Molecular Weight: 414.07 g/mole ID: H-24749 Date received : 10 September 2004 Expiration/reanalysis date : 12 October 2007 Purity: 99.2% Description : Solid Wildlife International Ltd . ID: 6849 Name : Heptadecafluorononaoic Acid, Perfluorononanoic Acid Synonym used in Report: PFNA CAS Name: Nonanoic acid, heptadecafluoro-(8CI, 90) CAS Number: 375-95-1 Molecular Weight : 464.08 g/mole ID: H7568 Date received: 30 November 2004 Expiration/reanalysis date : 12 October 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -20- Purity: 99+% Description : Solid Wildlife International Ltd. ID : 6944 Name : Nonadecafluorodecanoic Acid, Pertluorodecanoic Acid Synonym used in Report : PFDA CAS Name: Decanoic acid, nonadecafluoro (6CI, 70, 80, 90) CAS Number: 335-76-2 Molecular Weight: 514.08 g/mole ID : R1 1K Date received : 30 November 2004 Expiration/reanalysis date : 12 October 2007 Purity : 99+% Description : Solid Wildlife International Ltd. ID: 6943 Name: Perfluoroundecanoic Acid, Perfluoroundecanoic Acid Synonym used in Report: PFUA CAS Name : Undecanoic acid, heneicosafluoro-,ammonium salt CAS Number: 4234-23-5 Molecular Weight : 564.09 g/mole m: Q30G Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99+% Description: Solid Wildlife International Ltd . ID: 6945 (9CI) Name : 2H, 211, 311, 3H Pentadecafluoro-Decanoic acid Synonym used in Report : 7-3 Acid CAS Name: Decanoic acid, 4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-pentadecafluoro- CAS Number: 812-70-4 , Molecular Weight: 442.12 g/mole 1D:26833 Date received : 20 April 2006 Expiration/reanalysis date : Not provided Purity : Not provided Description : Solid Wildlife International Ltd . ID: 7592 Name: 1-Perfluoro heptyl ethanol, 7-2 Secondary Fluorotelomer Alcohol Synonym used in Report: 7-2 sFTOH CAS Name : Not provided CAS Number: Not provided Molecular Weight : 414.11 g/mole ID : 27497 Lot: 72SFTOHGA05 Company Sanitized . Does not contain TSCA CBI, Wrldlife .InteYnational, Ltd. -21- Date received : 20 April 2006 Expiration/reanalysis date : Not provided Purity : Not provided Description: Liquid Wildlife International Ltd . ID: 7593 Internal Standards Name : N-Pertluorooctanoic Acid [1,2-di-13C] Synonym used in Report : 2-13C PFOA CAS Name: NA CAS Number: NA ID : H-25617 Dates received : 1 September 2004 and 20 April 2006 Expiration/reanalysis date : 12 October 2007 and 31 January 2007 Purity : 96 .4% (not given for WIL 7591) Description : Solid Wildlife International Ltd. ID: 6827, 7591 Standard Reanalysis Date : August 2007 Purity: Confirmed Expiration Date: August 2010 Name: 1D, 1D, 2D, 2D, 3-13C-Heptadecafluorodecanol Synonym used in Report: HDFD CAS Name : NA CAS Number: NA ID: H-25433 Dates received : 10 September 2004 and 20 April 2006 Expiration/reanalysis date: 12 October 2007 Purity: 100% (not given for WIL 7590) Description : Solid Wildlife International Ltd . IDs : 6850, 7590 Project No.: 112E-108 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No.: 112E-108 -22- Test Soils Four freshly collected United States (U.S .) soils were used to evaluate the test substance under aerobic and anaerobic conditions . The soils were collected from each of the following soil orders : Soil Order Percent of US Land* Alfisol 13 Mollisol 25 Inceptisol 16 Ultisol 13 *Percent of total land in the US, from Foth, H.D . 1990 . Fundamentals of Soil Science 8 ed . John Wiley & Sons, New York) The soils were processed between 6 and 22 days after collection. Processing included removing vegetation, larger soil fauna, stones and passing the soil through a 2 nun sieve. The soil used in the sterile groups was sterilized by cobalt irradiation . The soils were stored in the dark at 4 t 2 C. Characterization of the soils was conducted by Agvise Laboratories (Northwood, North Dakota, USA). The following is a list ofthe minimum physicochemical properties of the soil. " Texture (i .e ., percentage of sand, silt and clay) (NUT .02.06) " pH (NUT.02 .05) " Organic carbon (NUT.20.20) " Bulk density (NUT.02.10) " Field moisture capacity (% moisture at 1/3 bar) " Cation exchange capacity (NUT.02.03) The percent moisture and water holding capacity (ISO 14240-2; Annex A) of each of the soil types was determined . In addition, the microbial biomass (ISO 14240-2; fumigation-extraction) of a live sample of each soil type was determined. The soil microbial biomass was determined at the beginning of the test prior to adding the test substance and in test and control soil samples at 4, 6 and 12 months using the fumigation-extraction method. Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No. : 112E-108 - 23 - Test Apparatus and Conditions The test chambers were 250 mL glass serum bottles (Wheaton, Milleville NJ). The chambers were sealed with butyl rubber septa (Wheaton, Milleville NJ) that were covered with aluminum foil and aluminum crimp caps (Wheaton, Milleville NJ). The test chambers were identified by project number, test substance ID, test concentration, and unique identifier and incubated statically at approximately 20 t 2C. Soil Pre-Incubation - The soil moisture of each test soil content was adjusted to 40 to 60% of water holding capacity (which is equivalent to a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar). The test soil then was allowed to warm to the test temperature for at least 2 days. A pre-incubation period of at least 7 days for the aerobic soils and at least 14 days for the anaerobic soils was performed under the appropriate conditions (i.e. temperature, soil moisture content, aerobic or anaerobic). Approximately 25 grams dry weight equivalent of test soil was added to the test chambers . Sterile soil treatments were dosed at approximately 200 mg/kg (dw) with both chloramphenicol (Aldrich, 98%, CAS 56-75- 7) and cycloheximide (Sigma, min. 94%, CAS 66-81-9) to inhibit microbial growth. The chambers to be incubated under aerobic c9nditions were sealed with aluminum foil-lined septa and incubated. The chambers to be incubated under anaerobic conditions were flushed with anaerobic mixed gas, sealed with aluminum foil-lined septa and incubated. The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC). The soil moisture content of each chamber was checked and adjusted, if necessary, once during the pre-incubation period by weighing the test vessels . Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water losses . Preparation of the Test Chambers The soil moisture content was checked and adjusted, if necessary, prior to the addition of the test substance . Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water. Anaerobic treatments and controls were prepared within an anaerobic environment and stored in a temperature controlled room. The test substance was applied to the soil within the test chambers as described below. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -24- Background Blank Control : Soils with no test substance added was tested in duplicate to check for background concentrations of analytes (see Sampling and Measurements) . The entire bottle was extracted after a sample of the headspace had been taken. Test Substance : The test substance dosed treatments were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated . The entire bottle was extracted after a sample of the headspace had been taken. Abiotic Controls: The abiotic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of 6OCo-sterilized soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition . The entire bottle was extracted after a sample of the headspace had been taken. Spike Recoverv Controls: The spike recovery controls were prepared by dosing approximately 25 grams (dry weight equivalent) of 6OCo-sterilized soil with appropriate volumes of 8-2 FTOH stock (250 ~tg/mL in ethanol) and fluorinated acid stocks (10 pg/mL in water). The stocks were injected directly into the soil using a glass microsyringe . The test chambers were immediately sealed with septum lined with aluminum foil and the contents of the chambers were mixed. The entire bottle was extracted after a sample of the headspace had been taken. Viability Controls (untreated): The metabolic activity of untreated test soil was assessed on a monthly basis. Duplicate incubation chambers for each test soil and condition (aerobic and anaerobic) were dosed at approximately 100 mg/kg dw with a combination of radiolabeled and non-labelled glucose . The evolved 14COZ (aerobic and anaerobic conditions) and '4CH4 (anaerobic conditions) was measured and the percent biodegradation calculated. Aerobic Controls (treated): The aerobic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight . The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated. The oxygen content of a sacrificial chamberwas measured at monthly intervals. Maintenance of Test Chambers The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC ; a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar) . The soil moisture content was initially checked and adjusted, if necessary, at 1 to 2 week intervals by weighing the test vessels but was changed to monthly intervals based on the observed weight losses . Adding sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) compensated for water losses . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -25- The oxygen content in the headspace of two aerobic control chambers was assessed on a monthly basis to ensure aerobic conditions . If the mean measured oxygen content within the control chambers was less than 19%, aerobic test chambers was aerated with filter-sterilized, oil-free air. Test chambers did not have to be opened to add water or for monitoring purposes . Soil moisture content was adjusted by injecting the appropriate amount of sterile water through the septa and onto soil . The headspace oxygen content was measured by inserting the probe of an oxygen analyzer through the septa and into the headspace of the aerobic control chamber. Sampling and Measurements Duplicate chambers from each of the control, treated and spike recovery soils were extracted and analyzed . Sampling intervals were at 0, 1 and 2 weeks and 1, 2, 4, 6, 9 12, 15, 18 and 24 months for aerobic. Potential volatile transformation products in the headspace of the soils chambers were collected using a C18 cartridge. At each sampling time prior to opening the test vessel, the septum was pierced using a needle connected to, a C18 cartridge and syringe. A 20-ML volume of headspace gas from within the chamber was pulled through the C18 cartridge using the attached syringe. The C18 was subsequently extracted with ACN and analyzed. Sample extracts were stored at -10C or lower if analysis is delayed more than 24 hours. Extracts were analyzed for: 1 . 8-2 FTOH 2. 8-2 FTA 3. 8-2 FTUA 4. PFOA 5 . PFNA 6. PFDA 7. PFUA 8. 7-2 sFTOH 9. 7-3 Acid Quality Criteria Recovery for a given sampling time point should range between 70 to. 120% for the analysis of the 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFNA, PFDA, PFUA, and PFOA from the spike recovery control samples . These ranges should be interpreted as targets and should not be used as criteria for acceptance of the test. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -26- Viability Assessments On a monthly basis, duplicate incubation chambers for each test soil and condition were dosed with 1 mL of the stock solution containing both radiolabelled and non-labelled forms of d-glucose which resulted in a concentration of approximately 3000 mg/kg dw. The headspace of each of the aerobic test chambers was continuously purged at an air-flow rate of approximately 1 bubble per second . The air exiting the test chambers was be passed through a series of gas washing bottles with at least one containing approximately 100 mL of 1.5 N KOH. The headspace of each of the anaerobic chambers was continuously purged with a flow of nitrogen and passed through a gas collection system consisting of two sets of COZ traps and a combustion apparatus. The displaced gases initially passed through one empty bottle followed by two bottles each containing approximately 100 mL of 1.5N KOH (COZ trapping solution) followed by another empty bottle . The gas was combined with a flow of oxygen and channeled through a quartz column that was packed with cupric oxide and maintained at approximately 800C in a tube furnace to combust methane to CO2. The gas exiting the combustion column was passed through an empty bottle followed by two additional C02 traps. The COZ traps connected to the aerobic chambers and the ls` COZ trap in each set (before and after furnace) of the anaerobic mineralization apparatus were removed for analysis and replaced at least weekly. Three replicate 1 mL aliquots of C02 trapping solution were analyzed for radioactivity by liquid scintillation counting (LSC ; Packard Tri-Carb Liquid Scintillation Counter) . The assessment was terminated when 50% of the initial activity was recovered as mineralization products or after 28 days . RESULTS Test Conditions Room temperatures during the acclimation period for all four soils ranged from 18 to 22 C. Temperatures recorded during the test for all four soils ranged from 17 to 25 C. Incubation temperatures outside of the range 18 to 22 C were observed three times over the duration of the study. The frequency of measurements performed on the test soils is presented in Table 1 . The moisture contents of the soils were adjusted as needed based on soil moisture content determinations. Each vessel required water additions at least once during the initial 12-month test period, but no additions were required for shorter incubation times. The maximum amounts of water added to any vessel over 12 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Proj ect No. : 112E-108 -27- and 24 month periods were 1 .2 and 3.9 mL, respectively . The oxygen content in the headspace of the two aerobic control chambers used to assess aerobic conditions is presented in Table 2. Characterization of Soils The test soils were characterized before the start of acclimation, and the results are presented in Table 3. The results ofthe viability assessments are presented in Tables 4-7 . The results ofmicrobial biomass determinations performed at the start of the test, during the test, and at the end of the test are presented in Table 8 . Sample Processing and Analysis Soil chambers were either processed immediately upon receipt or removed from frozen storage and prepared for analysis of the candidate analytes 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA . Headspace Analyses Prior to opening the soil chambers, a 20-mL volume of headspace gas was sampled from each chamber for 8-2 FTOH using a gas-tight syringe (Becton Dickinson, 10 ML with Luer-LokT"' tip), hypodermic needle (Becton Dickinson, 18G 1%z) and a C,8 cartridge (600 mg, Alltech Maxi-Clean) . Each sampled C,8 cartridge was extracted with 10 mL of acetonitrile (Burdick & Jackson, HPLC-Grade, CAS No. 75-05-8). The extracts were fortified with 1001tL of a 0.100 mg HDFD/mL internal standard in acetonitrile to yield an internal standard concentration of 100 ng/mL in each extract. The fortifications were performed using a gas-tight syringe (Hamilton 81000 Series) . An aliquot of each sample extract was transferred into a glass autosampler vial, sealed and analyzed for 8-2 FTOH by GC/MS. A method outline and typical instrumental conditions for analysis of 8-2 FTOH in the headspace ofthe soil chambers are provided in Figure 1 and Table 9, respectively. Soil Analyses Following headspace sampling, the crimp seal from each soil chamber was removed and the aluminum foil liner was placed inside the test vessel . A 125-milliliter aliquot of acetonitrile extraction solvent (measured with a graduated cylinder) followed by a 10-milliliter aliquot of 200 mM sodium hydroxide (measured with a serological pipette and prepared with Aldrich, Lot No . 14227T0 NaOH or Fisher, Lot No . 926471 and Burdick & Jackson HPLC-Grade reagent water, CAS Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -28- No . 7732-18-5) were added to each test vessel containing the soil sample . The acetonitrile extraction solvent contained 100 ng/mL of HDFD internal standard and was prepared by fortifying four liters of acetonitrile measured with a graduated cylinder with 400 pL of a 1 .00 mg HDFD/mL primary stock in isopropyl alcohol (Burdick & Jackson, HPLC-Grade, CAS No . 67-63-0) and a gas-tight syringe. The vessels were re-sealed and placed onto a gyratory shaker table (New Brunswick Scientific, Model G10) set for 250 rpm for three hours at room temperature . The vessels were removed from the gyratory table and allowed to settle for several minutes at ambient temperature . Using a volumetric pipette, a 10-mL aliquot of the extract from each vessel was transferred into a separate polypropylene centrifuge tube (Nalgene, 50-mL polypropylene Oak Ridge type, Catalog No . 3119-0050) containing 25 milligrams of ENVI-Carb (Supelco, 120/400) . The test vessel, including the remainder of the extract, was replaced into frozen storage (operating range -10 to -20C) . The extract aliquots were centrifuged (Sorvall RC5BPlus fitted with Sorvall SS-34 rotor) for 10 minutes at 12000 rpm (17000 g) and 20 degrees C. An aliquot of supernatant was diluted with reagent water containing 1 .00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water and the diluted extract was analyzed for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by HPLC tandem mass spectrometry (LC/MS/MS) . The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes . The dilution water was freshly prepared by fortifying 100 mI, of reagent water with 100 pL of a 2-13C PFOA secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and either a gas-tight syringe or calibrated micropipettes . A second aliquot of supernatant was diluted with reagent water containing 1 .00 ng/mL of HDFD internal standard and in the proportions 1 part extract: 1 part dilution water and the diluted extract was analyzed for 8-2 FTOH by LC/MS/MS . The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes. The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 1tL of a HDFD secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and gas-tight syringe. The remainder of the soil extract supernatant was decanted into glass scintillation vials (Fisher, 20-ml, disposable, Catalog No . 03-3377) and placed in frozen storage. Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No.: 112E-108 - 29 - At the request of the Sponsor, two additional analytes, 7-2 sFTOH and 7-3 Acid, were screened for in selected stored soil extracts collected prior to the Month 18 sampling interval using LC/MS/MS . The earlier sampling intervals included Day 0 and Months 2, 6, 9, 12 and 15 . 7-2 sFTOH and 7-3 Acid were also screened for in soil extracts generated concurrent with the Months 18 and 24 sampling intervals. The soil treatments selected for the screen included one untreated control soil replicate and the live treated soil replicates in each of the four soil types. Three different sample preparation regimes were used. For concurrent analyses of 7-2 sFTOH and 7-3 Acid, an aliquot of soil extract was diluted with reagent water containing 100 ng/mL of HDFD internal standard and 1 .00 ng/mL of 2-13C PFOA internal standard in the proportions 1 part extract: 3 parts dilution water. This preparation was used for Month 18 and batch analyses on the earlier sampling intervals, except Day 0. For 7-2 sFTOH analyses only, an aliquot of soil extract was diluted with reagent water containing 1.00 ng/mL of HDFD internal standard and in the proportion of 1 part extract: 3 part dilution water. For 7-3 Acid analyses only, an aliquot of soil extract was diluted with reagent water containing 1.00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water. The latter two preparations were used for processing Day 0 and Month 24 samples where re-analyses due to instrumental or sample stability issues were required (e.g. 7-2 sFTOH stability in the dilutions was suspect) . Each of the dilution solvents in reagent water was freshly prepared before use. A method outline for the analysis 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts by LC/MS/MS is presented in Figure 2. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA are provided in Table 10. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTOH are provided in Table 11 . Typical instrumental conditions for LC/MS/MS analysis of 7-2 sFTOH and 7-3 Acid are provided in Table 12. Stock and Calibration Standard Preparation Individual component primary stock solutions (1 .00 mg/mL) were prepared for each analyte and internal standard from the neat materials using an analytical balance and volumetric glassware. Individual component secondary stock solutions (100, 10 .0, 1 .00 and 0.100 pg/mL) were also prepared by serial dilution. The 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 2-13C PFOA and HDFD (for 24 month interval only) primary stock solutions were prepared in acetonitrile. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -30- The 8-2 FTOH, 7-2 sFTOH and HDFD (except as noted for the 24 month interval) primary stock solutions were prepared in isopropyl alcohol. All secondary stocks were serially diluted with acetonitrile . The 100-pg/mL HDFD secondary stock solution was used to fortify the internal standard into soil extraction solvent, calibration standards containing 8-2 FTOH and 7-2 sFTOH and dilution solvents for analyses of soil extracts for these analytes . For GC/MS analyses of 8-2 FTOH in the headspace gas of soil test chambers, 8-2 FTOH calibration standards in acetonitrile (25 .0, 50.0, 100, 250 and 500 ng 8-2 FTOH/mL) and each fortified at 100 pg HDFD/mL internal standard, were prepared from the appropriate individual component stocks . Representative ion chromatograms for 8-2 FTOH calibration standards by GC/MS are presented in Figures 3 and 4. For LC/MS/MS analyses of 8-2 FTOH in soil extracts, 8-2 FTOH calibration standards in 50 : 50 (v/v) acetonitrile : reagent water (10.0, 25 .0, 50 .0, 100, 250 500 and 1000 ng 8-2 FTOH/mL) and each fortified at 100 pg HDFD/mL internal standard, were prepared from the appropriate individual component stocks. Representative ion chromatograms for 8-2 FTOH calibration standards by LC/MS/MS are presented in Figures 5 and 6. For 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses of soil extracts, a 100~tg/mL -combined stock of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks . Secondary combined acid stocks in acetonitrile (10.0, 1.00 and 0.100 pg/mL) were prepared by serial dilution from the 100 ug/mL combined stock solution. Combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards in 25 : 75 (v/v) acetonitrile : reagent water (0 .100, 0.250, 0.500, 0.750 and 1.00 ng/mL) and each fortified with 0.75 ng 2-13C PFOA/mL internal standard were prepared from the 0.100-pg/mL combined stock. A 5.00 ng/mL combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standard in 25 : 75 (v/v) acetonitrile : reagent water was prepared from the 1.00pg/mL combined stock. All stock and calibration standards were stored under refrigerated (-4 degrees C) conditions when not in use. For 7-3 Acid and 7-2 sFTOH analyses of Months 2, 6, 9, 12, 15, 18 and 24 (7-3 Acid only) soil extracts, two sets of combined stocks were prepared. A 10-pg/mL combined stock of 8-2 FTA, Company Sanitized . Does not contain TSCA CBI, Wildlife International, Ltd. Project No.: 112E-108 -31- 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks . Secondary combined stocks in acetonitrile (1 .00 and 0.100 gg/mL) were prepared by serial dilution from the 10 .0-pg/mL combined stock. A 10 .0-[tg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile was prepared from the individual component primary stocks . Calibration standards in 25 : 75 (v/v) acetonitrile : reagent water containing 0.75 ng 2-13C PFOA/mL and 100 ng HDFD/mL internal standards were prepared from the 0.100 and 1 .00-pg/mL combined perfluorinated carboxylic acid (PFCA) and 10 .0-ug/mL combined telomer alcohol stock solutions, respectively . The nominal concentrations of 7-3 Acid and 7-2 sFTOH in the calibration standard set (7-3 Acid/7-2 sFTOH) were 0.100/5 .00, 0.250/10.0, 0.500/25 .0, 0.750/35.0, 1 .00/50 .0 and 5.00/250 ng/mL. Due to stability concerns, fresh preparations of 7-2 sFTOH calibration standards were prepared from the 10 .0-pg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile for analyses of 7-2 sFTOH in Month 24 soil extracts . The nominal concentrations of 7-2 sFTOH were as specified above. For 7-3 Acid analyses of Day 0 soil extracts, a combined calibration standard for set for 7-3 Acid and 7-2 sFTOH only was prepared as described above from individual component 7-3 Acid (0 .100 and 1 .00-ng/mL) and 7-2 sFTOH (10.0-ng/mL) secondary stock solutions . Only 7-3 Acid results were reported from this standard set due to a delay in instrumental analyses . For 7-2 sFTOH analyses, another set of standards was freshly prepared from the 10.0-p.g/mL 7-2 sFTOH secondary stock solution. Representative ion chromatograms for 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards by LC/MS/MS are presented in Figures 7-22 . Calibration Curves and Limits of Quantitation Calibration standard sets for a given analysis were injected at the beginning ano end of each analytical sequence with one calibration standard injected after a maximum of every five study samples. For 8-2 FTOH analyses in the headspace of test vessels by GC/MS, linear regression equations were generated using adjusted peak areas (for the internal standard response ratio) versus the respective concentration of the calibration standards. The concentration of 8-2 FTOH in the sampled headspace of the soil test vessels was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation . The concentrations were converted to a mass basis using the appropriate volumes, weights and unit conversion factors. A representative calibration curve for 8-2 FTOH analyses by GC/MS is presented in Figure 23 . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -32- For soil extract analyses of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA,7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS, weighted linear regression equations were generated using peak area response ratios (analyte :internal standard) versus the respective concentration ratios (analyte :internal standard) of the calibration standards. The concentration of each analyte in the samples was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation. Representative calibration curves for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses by LC/MS/MS are presented in Figures 24-32, respectively. The limits of quantitation (LOQ) for these analyses were defined on a low-level standard equivalent basis and were calculated for 8-2 FTOH in the sampled headspace and for each analyte in soil as the product of the low-level standard concentration and the dilution factor of the untreated control samples. The LOQ for 8-2 FTOH in a 20 mL headspace gas aliquot was 12 .5 ng/mL sampled headspace gas, or 0 .250 jig for a 20-mL sample . The LOQ was 2.0 ~ig/kg for soil analyses of 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA. The LOQs for soil analyses of 8-2 FTOH and 7-2 sFTOH were 100 and 100 jig/kg, respectively. Statistical derivation of the limits of detection (LOD) was completed for all analytes with the exception of 7-3 Acid and 7-2 sFTOH during method verification trials . The calculation for each analyte was based on three times the concentration equivalent of the standard deviation of five replicate analyses of a reagent blank. Method verification trials and derivation of statistical LODs for 7-3 Acid and 7-2 sFTOH was outside the scope of the study. Measured concentrations of the analytes are presented in mg/kg soil dry weight and gmol/kg soil dry weight in Appendix I and II, respectively . Measured concentrations of 7-2 sFTOH and 7-3 Acid in the untreated soils were less than the LOQ and are not presented. Example calculations are presented in Appendix III. Representative chromatograms are presented for each of the four treatment groups in aerobic alfisol soil . Similar chromatographic characteristics were evident for the aerobic ultisol, mollisol and inceptisol soils. Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of untreated control, fortified control, sterile and live samples treated with~ a from Day 0 of the test are presented in Figures 33, 53, 69 and 85, respectively . Representative ion chromatograms for Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -33 - 8-2 FTOH in the sampled headspace of untreated control and live samples treated withC- from Month 24 ofthe test are presented in Figures 43 and 95, respectively . Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of fortified control and sterile samples treated withL ~ from Month 12 of the test are presented in Figures 61 and 77, respectively . Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from untreated control samples from Day 0 and Month 24 of the test are presented in Figures 34-42 and 44-52, respectively. Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from fortified control samples from Day 0 and Month 12 of the test are presented in Figures 54-60 and 62-68, respectively. Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from sterile samples treated with L jfrom Day 0 and Month 12 of the test are presented in Figures 70-76 and 78-84, respectively. Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from live samples treated with L jfrom Day 0 and Month 24 of the test are presented in Figures 86-94 and 96-104, respectively . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -34REFERENCE Project No. ; 112E-108 1 Organisation for Economic Cooperation and Development. April 2002 . Aerobic and Anaerobic Transfortnation in Soil . OECD Guideline 307. p. 35 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -35 - Table 1 Frequency of Measurements] Interval Day 0 Biomass ,f Soil moisture Activity Headspace oxygen content Soil metabolic activit Analytical interval Week 1 . Week 2 ,f Week 4 ,/ Week 6 Month 2 Month 3 Month 4 ,f Month 5 Month 6 Month 7 ,J Month 8 ~I Month 9 Month 10 ,J Month 11 ,/ Month 12 ,f ~1 Month 15 Month 18 ,J Month 21 ~l Month 23 I Month 24 . Addit~onal chambers for the measurement of biomass and headspace oxygen content beyond the initial 12 month test period were not prepared at the start of the study . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Interval Month 1 Month 2 Month 3 Month 4 Month 5 Month 6 Month 7 Month 8 Month 9 Month 10 Month 11 Month 12 -36- Table 2 Test Chamber Headspace Oxygen Content Alfisol 20.8 21 .0 20.9 20.8 20.8 20.9 20.8 20.8 20.8 20.9 20.8 20.9 Average Ox Ince tisol 20.8 20.9 20.9 20.8 20.7 20.9 20.7 20.8 20.8 20.9 20.8 20.9 , en Content % Mollisol 20.7 20.7 20.6 20.8 20.7 20.7 20.8 20.9 20.8 20.8 20.8 20.8 Project No.: 112E-108 Ultisol 20.8 ' 20.7 20.6 20.8 20.8 20.8 20.7 20.8 20.8 20.9 20.8 20.8 p. 37 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 Soil label Texture class Alfisol Inceptsol Ultisol Mollisol Sandy loam Loam Sandy loam Sandy clay loam 1- cation exchange capacity 2- pH in 1 :1 soil water ratio -37Table 3 Selected Soil Properties Organic carbon content % Sand Silt Clay % 1 .4 65 24 11 2.0 45 32 23 2.9 55 31 14 2.1 59 18 23 CEC' meq/100 g 7.8 16 .9 7.9 17 .7 Water holding capacity pH2 % 5.8 54 .5 6 .6 43 .3 4.8 59.3 6.9 63 .2 Company Sanitized. Does not contain TSCA CBt . Wildlife International, Ltd. Project No.: 112E-108 -38- Table 4 Soil Viability by Glucose Mineralization : Alfisol Month Glucose Mineralization Rate Aerobic 1 1 .83 2 1 .79 3 2.45 4 2 .73 5 2.54 6 2.60 7 3.99 8 3.79 7.57 10 2.64 11 2.39 12 2.00 15 1 .64 18 1.81 24 1.81 - (percent biodegradathon/elapsed time m days) x 100 Anaerobic 1 .81 2.68 7.34 7.36 4.17 3 .00 NA NA NA NA NA NA NA NA NA Table 5 Soil Viability by Glucose Mineralization : Inceptisol Month Glucose Mineralization Rate . (perce. 4 t .: Aerobic 1 2.48 2 4.15 3 8 .60 4 8.90 5 8.29 6 8.97 7 8 9.50 9.47 10.46 10 8.91 11 8.50 12 8 .04 15 8.14 18 8.06 24 8.90 egradationlelapsed time in days) x 100 Anaerobic 1.95 3.89 8.44 7.76 3 .34 3.02 NA NA NA NA NA NA NA NA NA Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -39- Table 6 Soil Viability by Glucose Mineralization : Ultisol Month Glucose Mineralization Rate Aerobic Anaerobic 1 2.23 2.22 2 2.56 2.39 3 1 .67 2.86 4 1 .95 4.22 5 4.04 5.41 6 2.46 3 .80 7 3 .79 NA 8 7.33 NA 9 7.37 NA 10 8 .83 NA 11 2.99 NA 12 3.61 NA 15 8.19 NA 18 2.37 NA 24 3 .65 NA - (percent biodegradation/elapsed time in days) x 100 Table 7 Soil Viability by Glucose Mineralization : Mollisol Month Glucose Mineralization Rate Aerobic Anaerobic _ 1 2.36 1.85 2 2.54 2.99 3 1 .72 2.57 4 3.63 3.99 5 4.06 4.82 6 3 .66 4.16 7 3 .74 NA 8 3.95 NA 9 3.60 NA 10 7.51 NA 11 2.73 NA 12 1 .78 NA 15 3 .59 NA 18 2 .11 NA 24 1 .81 NA - (percent biodegradation/elapsed time in days) x 100 Company Sanitized . Does not contain TSCA CBI . Wildrfe International, Ltd. Project No. : 112E-108 -40- Table 8 Soil Microbial Biomass ([tg/g) (ISO 14240-2; fumigation-extraction) ALFISOL Aerobic Anaerobic Treated Untreated Treated Untreated Post Inc. - 244 - 128 Month 4 317 323 435 287 Month 6 680 279 593 645 Month 12 769 675 NA NA NA-Not analyzed INCEPTISOL . Aerobic Anaerobic Treated Untreated Treated Untreated - 131 - 132 138 109 184 106 432 483 423 467 504 564 NA NA Company Sanitized . Does not contaln TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -41- Table 8 (continued) Soil Microbial Biomass (Vg/g) (ISO 14240-2; fumigation-extraction) MOLLISOL Aerobic Anaerobic Treated Untreated Treated Untreated ULTISOL Aerobic Anaerobic Treated Untreated Treated Untreated Post, Inc. - 190 - 53 Month 4 216 218 218 205 Month 6 243 259 267 270 Month 12 714 734 NA NA NA - Not analy_Zea - 312 - 54 36 31 26 34 486 194 500 407 565 570 NA NA Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -42Table 9 Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas Instrument: Detector : Analytical Column : Injector Temperature: Oven Temperature Profile: Hewlett-Packard Model 5890 Series II Gas Chromatograph (GC) Hewlett-Packard Model 5971 Mass Selective Detector Phenomenex Zebron ZB-5 (30 m x 0.25 mm, 0.25-1.un film thickness) . 150C Initial temperature: 50C Initial hold time : 2.00 minute Ramp: Rate (C/minute,) 20.00 50.00 Final Temperature (C) 100 300 Detector Temperature: Carrier Gas: Septum Purge: Split Vent : Injection Volume : Data Acquisition Mode : Acquisition Ions : Peak Retention Times: 8-2 FTOH : HDFD : Final hold time : 3.00 minute 280C Helium, -1 mL/min; CHP = -14 psi Helium, -5 mUmin -40 mL/min 2 pL (2 stops w/ 10 AL syringe), splitless SIM Component Quantitation 8-2 FTOH 31 m/z HDFD 33 m/z Approximately 5.0 minutes Approximately 5 .0 minutes Confirmation 95 m/z 98 m/z Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -43- Table 10 Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts Instrument : Analytical Column : Column Temperature : Mobile Phase: Gradient Elution Profile: Injection Volume : Monitored Ion Transitions : PFOA 2-13 PFOA (internal standard) 8-2 FTA 8-2 FTUA PFNA PFDA PFUA LC/MS/MS Analog Parameters : Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source . Operated in the negative ion multiple reaction-monitoring mode . Zorbax RX-C8 (150 mm x 2.1 mm, 3-pm particle size) 40C A: 0.1 % Formic Acid in Reagent Water B: Acetonitrile Time min 0.00 1 .00 8.00 9.00 9.10 14.00 15 .00 20 .00 50 .0 pL Flow Rate lpL/min) 250 250 250 250 250 250 250 250 Percent A 70 .0 70 .0 15 .0 15 .0 5.0 5.0 70 .0 70 .0 Percent B 30 .0 30 .0 85 .0 85 .0 95 .0 95 .0 30.0 30.0 Q1 413.0 amu 415.0 amu 477.0 amu 457.0 amu 463.0 amu 513.0 amu 563.0 amu Q3 369.0 amu 370.0 amu 393 .0 amu 393 .0 amu 419.0 amu 469 .0 amu 519.0 amu Dwell Time 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec Retention Time 9.82 min 9.82 min 12 .2 min 12 .3 min 10.6 min 11 .2 min 11 .8 min IS = -4500 TEM = 400 DP = -10 FP = -140 EP = -10 CE = -15 CXP = -25 CUR = 8 CAD = 4 NEB = 12 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -44Table 11 Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts Instrument: Analytical Column : Guard Column : Column Temperature : Mobile Phase: Isocratic Profile: Injection Volume: Monitored Ion Transitions : 8-2 FTOH HDFD (internal standard) LC/MS/MS Analog Parameters : Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source . Operated in the negative ion multiple reaction-monitoring mode . THERMO EC Betasil C-18 (50 mm x 2.1 mm, 5-gm particle size) Javelin Betasil C-18 (20 min x 2,1 min) 40C A: 0.15% Acetic Acid in Reagent Water B: Acetonitrile Ti me min 0.00 6.00 Flow Rate (pL/min) 250 250 Percent A 30 .0 30 .0 Percent B 70.0 70.0 25 .0 pL Q1 523 .1 amu 528.1 amu IS =-4500 TEM = 450 DP = -25 FP =-250 EP=-10 Q3 58 .9 amu 58 .9 amu Dwell Time 500 msec 500 msec CE = -50 CXP = -10 NEB = 12 CUR = 7 CAD=6 , Retention Time 4.18 min 4.15 min Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -45Table 12 Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil Extracts Instrument : Analytical Column : Column Temperature : Mobile Phase: Gradient Elution Profile: Injection Volume : Reported Ion Transitions : 7-3 Acid 2-13 PFOA (internal standard) 7-2 sFTOH HDFD (internal standard) LC/MS/MS Analog Parameters : Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source . Operated in the negative ion multiple reaction-monitoring mode . Zorbax RX-C8 (150 mm x 2.1 mm, 3-pm particle size) 40C A: 0.15% Acetic Acid in Reagent Water B : Acetonitrile/ 0.15% Acetic Acid Time min 0.00 1 .00 1 .01 7.00 12 .00 13 .00 20 .00 Flow Rate (pL/min) 250 250 250 250 250 250 250 Percent A 95 .0 95 .0 70 .0 15 .0 15 .0 95 .0 95 .0 Percent B 5.0 50 .0 30 .0 85 .0 85 .0 5.0 5.0 50 .0 pL Q1 441 .0 amu 415 .0 amu 473 .0 amu 528 .0 amu Q3 337.0 amu 370.0 amu 59 .0 amu 59 .0 amu Dwell Time 50 msec 50 msec 50 msec 50 msec Retention Time 11 .6 min 10.0 min 11 .9 min 12 .1 min IS =-4500 TEM = 400 EP = -10 CUR = 7 CAD = 6 NEB = 132 DP = FP = CE _ CXP = 7-3 Acid/ 2-13 PFOA -40 -350 -15 -11 7-2 sFTOH/ HDFD -25 -250 -50 -10 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -46- Project No.: 112E-108 Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers For each sample, fit a Cl$ cartridge between the end of an appropriate volume syringe and a hypodermic needle . With the plunger fully depressed, insert the needle of the disposable syringe through the septum of the sample and pull a full syringe volume. Remove needle, expel headspace air and repeat. Detach C,a cartridge and place in appropriate holder. Attach the barrel of a 10 mL disposable syringe to C,8 cartridge and use a volumetric pipette to transfer acetonitrile extraction solvent into the syringe barrel. Insert the syringe plunger into the barrel and inject the acetonitrile through the Ci8 cartridge into a 20-cc glass scintillation vial. Fortify the extract to contain 100 ng/mL 1D, 1D, 2D, 2D, 3-13C Heptadecafluoro Decanol internal standard. Pipette an aliquot of the extract directly into an autosampler vial . Cap vial and submit for GC/MS analysis of 8-2 FTOH . All sample vials not analyzed immediately are place in refrigerated storage. Store the remaining trap extract in scintillation vials under frozen conditions . Figure 1. Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers. Company Sanitized . Does not contain TSCA CBI . Wrldlrfe International, Ltd. -47- Project No.: 112E-108 Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts For each sample vessel, remove crimp seal and place foil liner into the vessel . Using a graduated cylinder, measure and transfer 125 milliliters of extraction solvent into each sample vessel . The extraction solvent consists of 100 ng/mL 1D, 1D, 2D, 213, 3-13C Heptadecafluoro Decanol internal standard in acetonitrile . Add a 10-mL aliquot of 200 mM NaOH into each sample . Re-crimp each sample vessel with a new aluminum crimp cap and the original septum . Extract the samples for approximately three hours using a gyratory shaker table set for 250 rpm . Allow suspended material in extracts to settle. Remove a 10-mL aliquot of the extract using a serological pipette and transfer into a separate 50-mL plastic centrifuge tube preloaded with approximately 25 mg of Envi-CarbT"' . Centrifuge samples at a setting of 12000 rpm for 10 minutes. Transfer extract supernatant for each sample into a separate labeled scintillation vial . For 8-2 FTOH analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL 1D, 1D, 2D, 2D, 3-13C Heptadecafluoro Decanol (HDFD) internal standard to produce a final solution composition of 1 :1 (v/v) extract: water. For 7-2 sFTOH analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL HDFD to produce a final solution composition of 1 :3 (v/v) extract:water . Perform dilution directly in an autosampler vial, cap and vortex . Submit for HPLC/MS/MS analysis of telomer alcohols. All sample vials not analyzed immediately, place in refrigerated storage. For PFCA analyses of each sample, dilute an appropriate volume of the extract supernatant (or diluted extract, ifapplicable) with reagent water fortified with 1 .00 ng/mL N-Perfluorooctanoic Acid, [1,2 di13C] internal standard to produce a final solution composition of 1 :3 extract: water. Perform dilution directly in an autosampler vial, cap and vortex . Submit for HPLC/MS/MS analysis of PFCAs. All sample vials not analyzed immediately, place in refrigerated storage . Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Store sample vessels and extract scintillation vials under frozen conditions . Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts . p. 48 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -48- Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : T :\DATA\ALFI025\0101005 .D DW 20 Apr 106 5 :41 pm GCMS#1 TA-30 25 .0 ug a .i ./L 1 un ance 1500 Ion 31 .00 (30 .70 to 31 .70) : 0101005 .D 5101 1000- 500- Time-->4 .00 Abundance ' 1 250 '4 .20- 4 .40 4 .60 4 .80 Ion 95 .00 (94 .70 to .. 5 .00, r5 .20 .5 .40 95 .70) : 0101005 .1) 5 d01 . 5 .,60 200~ 150 - Figure 3. Representative ion chromatograms for 8-2 FTOH in a low-level (25 .0 ng a.i ./mL) calibration standard by GC/MS . p. 49 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -49- Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Miac Info : Vial Number : T :\DATA\ALFI025\0501009 .D DW 20 Apr 106 7 :03 pm GCMS#1 TA-34 500 ug a .i ./L 5 A ndanc bu L', 30000 - Ion 31 .00 (30 .70 to 31 .70 : 0501009 .D 4 .199 20000,, 10000- Time-->4 .. 00. . A un ance 30001 . 4. .. 20. . . 4._..40. . . 4. .. 60. . . 4. .. 80. ~ Ion 95 .00 (94 .70 to .. . 5. .. 00. . .5. .. 20. . . 5. ..40 95 .70) : 0501009 .D 5A00 . ., 5 .60 2000- 1000 - Time-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 Figure 4. Representative ion chromatograms for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/MS . Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. -50- 'nY"MUP. .. .. -~.. ; wiii .P n. n. un :a1ie t ~i{ 7 Project No.: 112E-108 S t IM B Figure 5 . Representative ion chromatogram for 8-2 FTOH in a low-level (10 .0 ng a.i./mL) calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -51- rra..^~~~,Ea~ ... _~ ..~.,~.. :;. ,a. ..r:, ~. . ., . Project No.: 112E-108 *. n: Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -52- Project No. : 112E-108 ,.r..-~ .,..._ .. A . . -A Figure 7. Representative ion chromatograms for 7-2 sFTOH in a low-level (5.00 ng a.i./mL) calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -53 - Project No.: 112E-108 ~z~, .,a . . ....-, - --- _ -- . Figure 8. Representative ion chromatograms for 7-2 sFTOH in a high-level (250 ng a.i .1mL) calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -54- Project No. : 112E-108 R .YN:n :'~3~' 'syC~sLrmMar'sI.Glbn' hn0" bc' !1 )tql . lyp. +euJ .r latlmr o.10e v ..1 ./L C ]Nl.tr Cmv. t. t ! +y . .f ./1. ~ A .~q. D7lr~ ~i ObO~lM Nrulr. mv . Aql~lte 7w Yuly.! Cl .ule Yr .u7lw.4vld. SN.~WIdr ,IMS . Nr ++.~.,. l.u.elpceee. . 4. r. .k t+cf .. ~lt t ry. e a.oi i+ IF-,_ 7.7:e IT. :sus.s.e.l. 'ioi'sa7sm ue. . .f. mdHw . O .( rn f007 t0o7 . 0301 eem eM um ~sy 1001 7007 ]69 310 71m 3 7em r 2m 71m mm !m I7m lOm a 1 173. sos ~ ~ s . 1 s nn !sm n~ sn na an n u !s n -181 . Figure 9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildrife International, Ltd. -55- Project No. : 112E-108 'Hrt.t.N.n.ace."~:]aRalapGm~F.tI+t'.aw]a~a:]aol~wauw~rtGnn.a " ICA6L" ~nN"bt' l~la M.al smqt np~ RW00 up . .l ./L ~ I ...t aN ar . . 1 e y . .l .ns 4q . Dan aa AA " Tls. Of.3~'. SO !N XNllIN . ip w]y.e Mu .l. ~w l 7Ae t er, r.:w7~a1+1._~r".wettt- 7.IN 70N ]!N r~.mta, u~n e t~. w.D .. x~eltonet.~ Op . .t 4CIn . 4, . v . . c~stN c, w 41afv . en ii .7 .4 xe ya. be~ela,Tlr . tu, ie t7 .7 M171~t . 1~7aae. 7 s... .fe mttt7. ,e .e ]!N vN ]!N .a :aN ]AN 7]N bd Tlrlr~ I] . 7]a ae ].IN ]ON iDN 11 .t 17N 1/N Ibl V tAN 1>.1 1La 1 .IN ILI M~m. OOD). 700 . fOm. iqD . aom . ]Om . ]00 10m. OD 1 17M . . . . ._ p 11 Y O N 1F 1D 17 1! 19 Figure 10 . Representative ion chromatogram for 8-2 FTA in a high-level (5 .00 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife,International, Ltd. -56- Project No. : 112E-108 la.. :~~...., Figure 11 . Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -57- Project No. : 112E-108 3 . .. 1 '1 Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. p. 58 Company Sanltized . Does not contaln TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 m~ r, .. .~. : PekNin:'67e11 iJSy4)'14Y770.N~ Canet'IC1bM hnGYtn' lW7. ladu. a~b Syqq ruM.d uy nt ./L -1.ua tent . e.mto7 . ..7 .ns at9a~. Ti::~ ouaieiriu xdltfN. Ne qyzRk.~ avaiy+t elwtc YelulTlvublA . Y.. TAm.bia . .M... lmKT.~ IW. YleH~ s4" X.fgM. ~, ra iio~ ~ .U R7 .a1 e .os u..at.aaai.: IT, ve ry. aT. i:eetTtm~Ttm . e~i7T.ie:in "tlW~t . ilmi eed 9m. 1 ~.p 7ttL77t .in r 17 .5 Wv -58- fON~ aeN UN 44214 7eN 7aN 34"' 3270N 21N_ !6N' 23iN+ tON+ UN~ I!N t/N 13 .1 tb! AMA C0. a001 . 70m. 00 i i -i 17~0 i__T_- n v tt 7e te i) is u Figure 13 . Representative ion chromatogram for 8-2 FTUA in a low-level (0 .100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -59- Project No.: 112E-108 q Pm~ wqp.-s.psw .yr r iwj.n ~.evaswr 'q.tNw:'LYpT ~lW~1'19D!lIO.M _w Cmna'ICl69' M.N "Ix " 7.~i. LryJp u. 7i lt~AO vl ..l ./4 . c~.nlctntlm . IwLtN Cmc. t fl y..1 ./t9 71N ~ lq. T ls CiH~30 d Xaditld. stae. dtsvue.. elit. fstur i ud.. ~...Lle~ a_t.. rl ..a.u . .aetY. 1q.Yldtb . aY" f.lpN . Cy.1 .'44t1e~ Y. L.tiTa v .la ut .u slei f.ld n..at m. tp o~p. V.7 . iHOr ~ w tlnautrt... ltr . eT Ylndw~ ao.a .t. 77 .6 Yas YaN 7/H 73 .! 30 .! ue 77 .s 7.f.s /.. . .. ILW .t~ tttt "itn. 7a$ n.. . uf .t . bd Tlr~ ll .l Yn IIN 114. 1!.! 'r fes eZ u4 us IaN Lb! 1 1.5 !ICN TON Is . l11N 4- I710 I's _ 1! -- Figure 14 . Representative ion chromatogram for 8-2 FTUA in a high-level (5 .00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -60- ,.. . ' ' . ' s : .r. Project No. : 112E-108 Figure 15 . Representative ion chromatogram for PFOA in a low-level (0 .100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not .contain TSCA CBI . Wildlife International, Ltd. -61- Project No. : 112E-108 . .,v -=::1; 1 IXX . i w Figure 16. Representative ion chromatogram for PFOA in a high-level (5 .00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . p. 62 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 - 62 - dnw ..Tf .Ta._ +Fxrox w PUkfOrM99ACtl" Muf(u) "u]oxnomvf Canwf 1C1M.11" Ncdaim- efl . 7 - iaN - C.+ a . r.e . . os .o.~na re _ OOM .Iw eu ~n .na r [ ~f xaa~nn"~ ~. .Vht~ cuk aa<to . [<P ~~1 . r ~ ..f .cta! kt"tt tu cau.v. M~ 0 .5 ~b an n.n ,n m ~ru,f ., m0 i. ., 71 .a em " ft .n faw .L1. . nc "ON[ , ] L1 34- ] .N{ ]7N 70N 21M a 7EN S Iaw iii 77M IM 1 Na 1M1 17.1 t ON l000 0 IW00 10000 7J~00 00 n u 7] u v Is 1-7 ^-,I- Figure 17 . Representative ion chromatogram for PFNA in a low-level (0.100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. Project No. : 112E-108 -63- --- - -- ~- --- ----- ------- P.ANIn'ClIM-WrP(.P,Y!]SYIYUaN r.a. .wr unu.a- w.ara,- -P7. a... .-P_.. 7 .FU ud i . /~ a7cal.r .f i C n .7 ./nP 7,/7e0 " 7 -- ------ -- --- - SM! wda,.Ji Alnntnn. M.7,vr Clu.m n~iea~ u ".atn:nn . .an.~ ]f7 .t . E0" r7 ~ 1 .r. .eP . .., .,~ . . .7 . u. r~:~ ew . nin Famt~on'Lrn " .lori " f nn., 11 u . " n~n lo .n erc 7l77 x7 .5 xw 745 xa! x]a 7L7 71.7 7.07 ,43 14S I7.5 19.3 11.5 17.5 IN! 11 .3 145 PON !SN 70N eow !W ISN ]41 70 .1 1M t10 I 7 ] ros ! " 10 11 ,x Ix - ,S 1. 17 ~ L -~IY_~ Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -64- R4.IWn CtOA.'a' 4m(xl'!1]0'K90am! ' tmvnrl tCMF,!' Inaxav - 'Pls 1 . . 7+`q ~ ./4 Aeq. Y e.HVVnwvj a.w~.w r+ faui~" A1 q vn .nn~ i Uune ~i~inro e . 1 . Allw . oer. aU.. .ynt~ uo" ail k ~ Cxp. Pd . ! v~ . nNN~ . nn Lv r _.~ rm ta .n " tuc n ~ ii coa~ivn u u.e ~.n OAN !9N 0!N !aN !!N !ON . .e INJ 16N aaN 1!N a a9 !9N 7aN OaN !7N ; !ON LLL( 29N !RN 1aN 17N fM ,aN taN 17N 1 GI WWo t000 o 4- Project No.: 112E-108 Figure 19 . Representative ion chromatogram for PFDA in a low-level (0 .100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -65- Project No. : 112E-108 M7kH7we'G,O/aT k1s(rl'~+~oaio.n,u ' Cm .r~i 1CUta]' enalus,"epl" 7 ` : r T "ca . Tlnu o~ivv~eo.' o " ~Hifo M - ~Alps7 mlly., cLw~ M..~i'n.+.neie : =r. IZ. 1-112i, "'T -1 Iukertle~ FrY. A-7 . 11 V 1. R ~ Fx..~uC kire' i~nv" RT 7vP"r 79nrr on T 1 U~7 .= v. . m tn .n duvt 7fe"i Fnd pn "r 17 .0 .nn - " - ]iee ]q3 7NS zen 2 ases S _ e aa7 " S 22,5 21 . I- 1 .5 14 17 " 5 s~eE les j le " S f 1.44 1] "5 +as I 1 "5 1 .8 ao." F ok1 7 al eow 60N 70N ]ON tOkt 1M oo 1 ] . ' en +a u u n +s s + +i Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i.lmL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlrfe International, Ltd. -66-. Project No. : 112E-108 ' cfe'1s.1Y~ .iH6vu~ly.~e~.IlrrCw-M.tvWl~em.>-~6616616U .mV - ini... . .ci . r ,L cvl.a.flten ....: y . iaen nSw .~w M Inmla+a. lm.un-.~ x. an .lva .1 ...1 . nvlaJ~ u tH ~Il~ev .is , vai h~n" uk x GP. M7, e 1. u ~ GF .e1eJ . v e.LUV. Fh 4 .11 v.1 nn !. v Int . rypu Ibtmuvn ia t tv _i .o 6 Lm . rs u .l mn - - 6w tw +w I~N cxN . .e cw 7eN ]w O1N J7N 7w 7w 7w x6N uN xoN l6N lw IJN 11N lw F606 6UW0 ~6760 %60U 06 / 7 6 "-'~^r^~P y 11 , `12 1) it 16 16 N 19 Figure 21 . Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . p. 67 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 D..4Nn..'GIIJSkf We(..{ '600610U .mrf wnun uras.xl- a.wlo .- "~st].. 7+nio. . :.].a ..a i~a : i.n.r niianooi' ' nos~rso sl rada udr RIrPn.R+r RnalY.t <luqc 9 Da:mn .Ilnn.nold . m...n.]a . v "-..n.r 4 .D .~w H .',gntr [ap : vuk Rumr GrD . Ad1 . R v .l . R r o .r . uf.cvd Rl .t+ 7A tnR+n W. ROa+v. RTr Na n n An..u H.r9n .r v.ll .y ] 'i .iD~olnte p,d 7m.r 17 .i arn 1 x ws 17H16 .5 1ss" 74N" 21.5" 11.5" ILpi SM 1M 1 .0 17.5 tas ; Isa E +an !!! IUs us tla toe ow e ow 7 ow 45 M 4m SON 70N tON ao -67- nu s T--'-T"'-'i't-' ioTl IS IS IA Is Is 17 +u u Figure 22 . Representative ion chromatogram for PFUA in a high-level (5 .00 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. -68- Project No. : 112E-108 700000 T 600000 a a V 500000 cd a d 400000 - pb" 300000 - 200000 d 100000 0 +-i ; f--r 0 100 200 300 400 500 600 Concentration (gg 8-2 FTOH/L) Figure 23 . Representative calibration curve for 8-2 FTOH by GC/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -69- Project No. : 112E-108 M 042406_M12_534rcb(8-2FTCHk'LYsa"P4@grfssiai("1/)PHeglthgjy=1 .2xa0.00Bm(r=Q99W) 12.0 11B 11 .0 10.6 10A fl5 80 a5 7.5 7.0 fl a0 66 60 4.5 <0 36 3o 26 20 1 . 6 1 .0 Q5 ~/ QO ~ ,i /% 1 2 =' f i/ 3 4 6 9 Preyle Qxe l ISCarn ~rJ i ~i / .. " i 7 8 9 1o Figure 24 . Representative calibration curve for 8-2 FTOH by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -70- Project No.: 112E-108 " 052207_W4_8~TeTALMb -2FTOH) :' erarReprewbn('1 x'welphin0l:y' 1.91x+-0 .0272(r-0.9988) 5.0 49 4,5 4A . 42 4.0 3.83,634 323 0. 2J8 216 2A 22 2.0 1.81 .6IA 12 1 .0OA 015O A02- 0.1 / / ' f / /~ . % % / ./ 02 0.3 0.4 0.6 0.6 0.7 0.8 0.8 1 .0 1.1 12 18 1.4 1.6 AnaMe Coned 13 Gonc. ~ 1.8 1 .7 1.6 ~/ ` ~' 1 .0 2.0 21 22 ~ / _ 28 2.4 2.5 Figure 25 . Representative calibration curve for 7-2 sFTOH by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -71- Project No. : 112E-108 M 042003101 2 TELALAE.rdb (&2 (S)) : "Ltriear" Regresslon ("I / e weighthg): y=0.118 x 4 0.00123 (r = 0.9992) 0.80- " 0.75- 0.70- 0 .650.60 - D 0.55 7 _ 0 .50 < m 0.45 D N U.40 m 0.35 J j ./ ~ . i~ ~ / / / ~ l m D 0.30 d 0.25 ~ fi . 0.20 r,r i 0.15 s 0.10 0 .05 0 .00 ~ 0.5 1 .0 1 .5 2o 2.5 3 .0 3 .5 4 .0 4.5 5.0 5.5 8.0 AnalyOa Conc. / IS Conc. Figure 26 . Representative calibration curve for 8-2 FTA by LC/MS/MS . t 5.5 p. 72 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -72- Project No. : 112E-108 ~ '111~M5Stdlt~Wtxf~(73FfPj'l'ra~'F~~('1/~Yv~ti~ 40 35 30 25 20 . -- / / i/rf rf / I " ." % 1 J , f' 0 0 05 10 15 20 25 30 35 q0 45 50 56 60 65 Ikt~(De/19(b~ Figure 27. Representative calibration curve for 7-3 Acid by LC/MS/IVIS. Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. -73- Project No.: 112E-108 ti 042006 M12 TELALAE.rdb (6&2 (U)) : "Linear' Regression ("1 /X' weighting): y =1 .07 x+-0 .00685 (r=0.9996) 7.4 7.0- 6.5- 6.0i 5.5- D 5.0- m < 4.5 . 4 .0 m' d 3 .5 j ' i / 3 .0 D % w m 2 .5 2 .0- 1.5 - i 1 .0- rt 0.5 0.0 r 0.6 1 .0 1 .5 20 2.5 3 .0 3 .5 4 .0 4.6 5.0 5.5 AnelyEe Conc. I Is Conc . f !" ,- 6 .0 6.5 Figure 28 . Representative calibration curve for 8-2 FTUA by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -74- Project No. : 112E-108 042006_M12_TELALAE.rdb (PFOA C-8): "Unear' Regression ("1 I)eweighing): y=1.12x+0.0538 (r = 0.9985) 7.5.f 7.0- 6.5- 6.0- 5.5- D 5.0- m m 4.5 - D 4.0 - m w 3.6 /r / i ' / D 3.0 m 2.5- 2.0 - 1 .5- 1 .0.f~ 0.5 , 0 .0 ~ 0 .5 1 .0 1 .5 2.0 2 .5 3 .0 3.5 4 .0 4.5 6.0 5 .5 6 .0 8.5 Analyle Conc . I IS Conc . Figure 29 . Representative calibration curve for PFOA by LC/IvIS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -75- Project No.: 112E-108 042008 M12 TELALAE .nib(C-9Add) :"Unear"Regression("1lewelghthg) :y=1 .34x+ .0.00838(r=0 .9991) 9.0 - 8.6- , 8.0 - fr i 7.5 ~ i 7 .0 8 .5 r D 8 .0 < 5.5 rDv 5.0 r i m 4.5 4.0 /' D 3.5 m m 3.0 2.5 , 2 .0- i 1 .5- 1.0 " 0.5 - 0.0 - ~ 0.5 1 .0 1 .5 2.0 2.5 3 .0 3.5 4 .0 4.5 5.0 5.5 8.0 8.5 AnalyOa Conc. / IS Cone. Figure 30. Representative calibration curve for PFNA by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -76- Project No.: 112E-108 M 042006 M12 TELALAE.rdb(C-10Acld):"Linear"Regressbn("1 I e%elghtirg) :y=1 .4x+-0,0404(r=0.9996) 9.5 - 9. 0 - 8.5- 8 .0- 7 .5 - 7.0 . y 6.5 i , .. d 6.0 < 5.5 D 5.0 - m 4.5 - 4.0 - D m 3.5- m 3.0 - ' / ~ ` . i 2 .5- 2.0 . /~ 1 .5 1 .0 i 0.5 0.0 0.5 1 .0 1 .5 2.0 2 .5 3 .0 3.5 4 .0 4.5 5.0 5 .5 Analyle Conc./ IS Cone. Figure 31 . Representative calibration curve for PFDA by LC/MS/MS . / 6.0 6.5 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -77- Project No.: 112E-108 04200 6M12 TELALAE .rdb (C-11 Acid) : "Linear' Regressbn ("11X' welghting) : y= 1 .29 x+-0 .0743 (r=0 .9994) 8 .9 8 .5 8 .0 ,% 7 .5 j' 7 .0 6.5 i y 6.0- e 5.5 - m 5.0 - D w 4 .5- m 4 .0 - 3 .5- m 3.0 - 2.5- / / i % i ,' 2.0 - 1 .5 - 1 .0 _ 0.5 .~ 0.0 ~ 0.5 1 .0 1 .5 2.0 2 .5 3 .0 3.5 4 .0 4 .5 5 .0 5 .5 6.0 6.5 Analy1e Conc.l IS Cone. Figure 32. Representative calibration curve for PFUA by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No. : 112E-108 -78- Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C:\HPCHEM\1\DATA\ALFI005\0701011 .D fjl 30 Apr 105 GCMS#2 4 :10 pm 1570-TEX 7 Abundance Ion 31 .00 (30 .70 to 31 .70) : 0701011 .D 3000 - 2000- 1000 - {riL i f Ti-m ->e 4 .0 0 44' ..22'0 Mbund-ance 4 .40 4 . 60 Ion 95 .00 4 .80 5 .00 5 .20 5 .40 5 .60 94 .70 to 95 .70 ) ; 0701011 .D 5 .80 1000- 500 - Time-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 Figure 33. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,g cartridge extract) from a Day 0 untreated control soil by GUMS. 8-2 FTOH was not present (no peak at 5.0 min) . Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -79- Project No. : 112E-108 Figure 34 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS . 8-2 FTOH was not present (no peak at 4.18 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -80- p. 80 Project No. ; 112E-108 Ie ..-,- 3 i____-7.-"._-T----f ~. .........-... ...~+`^ti .,~+.,J ha'..-. .. . ..h1"~ 7`- -l Figure 35 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS . 7-2 sFTOH was not present (no peak at 11.9 min). p. 81 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -81- Project No. : 112E-108 p.. ...NoN.:.""m: "" 6-alRr "- 'i'Y:Wi8:'tidiv i+:.'otiiaL6u_Tiut.(r:..Ar--~ ut.wM"anaaao .n.." -- eenNnnc1nxe.tow.,w.aautcr ~ - a.Npl. 7nd.. : 14 +a.ptt T70.1 s1.w.t eme.nta" ctem 0 .09 w "" a " /N t"leulatM emc : N/A ,IAMca..ynDta .uN : 0o+T/aiso/:OoS+ m "WOOl : Mcdltt .d : xo Yol.. 7N.. .nwd, 7+ .et ap. 1 ... . W.l -: 1 :0 .71 cp. ~! Nr HeetAa : 1 Mom . , R7 Y>ndw: 7p .Y UP -4 N71 Lp . Via- 13 .7 0.70 Wn YOOOOa a.p " YdOht: ~~ eap . AdJ~ Rala~ II6Np. V" 1. bt7o : ~I Va. Rduaw RT : 0 .01 1 .00 l .ap No 7600m . .trnT1M'il~ . : Atul NopUt : ltut TiW:d Ti-, N" .11.1 N/A Nb. N/A NIA OounCa ~ln n0 "f0>00 G. 460000 >weoo: >seem:: MOAD wow Printing Date: Friday, April 22, 2005 aa ~~~ R 7 8 ~ tp.T~ +e ' 11 -" t 12 ' +3 14 16 i~ -- 7i ' u'7o- Figure 36 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . 8-2 FTA was not present (no peak at 12.2 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -82- Project No.: 112E-108 ay 3 . ..J d Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 7-3 Acid was not present (no peak at 11 .6 min) . p. 83 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -83- Project No.: 112E-108 P++tNwY'0-7IUrtLtlttlw)YSTOf.00.mif CamnI r.nl_'tIX-7W.f00.1fp~UfCY NwYFrr" lupl . 11 N F+ .pl " Typet Ftanl Canemcutlent O .OD v0 +-l .IL IOMS))) I: Ca1cW FC"d cmn I'M / / Ac9 . O+b : DI/17/D3 ' I~ACq . Tls : . D7t10:01 tN ~~I1 j N~tll"dl Ne UIn0 Y .etocl 1 II Netf+ TRtafMld . 201 .03 cpa Mt" 7Dte+RelO : 1009 .61 eps I Nu . . leeetl :a : 1 II XT ]D JI 6aptYlndettdawRT: .1.20] 1n+c i S" p- YtdtRi 0 .]0 UP . NtiORt : 0 .01 ~ E+p . tstt PK1ot S .OD i Fep . Ad~ . t+:1e . 1 .00 ~ ; 6ap . Vet . patlet ] .OD ~ Ut+ R"Ltlve RTt No 900.4 OOOt1l 76pN1 704. i li Int. TYM. Rstent3en ~iAtut I~ NetqAT : ' ltat Tlea : il Fnd T ta : No t"al N /R NfN N/A N~R N~R ~tn <OVnta t Lit, 6 ~4 ~ D.aDtl ; ~ D.DaI~ 1 FD.1 ~ 40~4 >tsD". sDa. 1eo.. :DD.. t tot. ~ tow. , 6M.00 ; ' MOOi - . -- -, t6a!-,-.~t .1yF.. ~ .~, 10.15 TT .tp Tan. 1an_ laa _ - I; t i ~ i-6 F 7 0 0 10 11 1] 17 id if - ie ni F 1'0 - - - -- ~ ~--~------- - ~ ~ ---~----- ~ ~ -------- TJ--t"= --~ ---- ._ . . . . .- -----1 Printing Date: Friday, April 22, 2005 -- .------~ --- ----------- ~- ----- - - Figure 38. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 8-2 FTUA was not present (no peak at 12.3 min). p. 84 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 P a :.~IBFeimp,e%-iD7ff"Yf' c...ONpN.tRmt:":rvacf.w+oce.r+ao.+wtwaM.tCtr"nAxMmesweo.n."n".r , .. aap.pli t.w.n lf Ii 7Tp. : e01"nR Cu.cmtutaen! .00 W a .l ./L LOOISI I~ GaleuLt"d OOncs N/A Atq . Oatu : Aeq . Tlwai Of/01/0! 07i10 .01 fN p10t1 . . NeA1f1t0 : No ,i e0nMlnq fa<teci 1 LOq" i ~ A[.a~Th["MOIGI~ ~Ma . f"pothn Rt Y1Mws ~~ LapteNd RIS ., Mp . "I 1h, up . NUqhts 4p . N.l Maw : I E[ p . hG~ . M[Sa1 Wp . Val . R.clel I V[ . R.lattve RT : 10.11 ! 10.0 9.12 O.tO 0.01 3 .o0 1 .00 i.Op Ib c[Dpa' a.c " cn l60N ; T . : AO : ' . Typ.i Mtfntion 21 ".: Ac.n N[Ipn[7 [ Les GM TI "fl NO put N/A N/A N/A Y/A N/A aun0a tpt "ln "ln 0oft,! I O SQON I -84- . i . . , zoo.4 t so.. +~" I xaoo ao " 1 - i'TM+,oMn +.W ..: nis le't tW. .^. .i.--n "--~d Figure 39 . Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . PFOA was not present (no peak at 9.82 min) . p. 85 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -85- ~'Nl+~nl~tEil~I~I,000' N I . !: II~Wa4N .mr. "eiAdf WWla): "ItfW7e.0ainu" GunnlR "117[ "t0.010B,11P1UTCY MlebOwt- i a .upl! ItW.ai ~'aa .pl! Typl : ICOnuntwtaen+ ~Glcul .l.d C.IACa . Dat.e ~Iwq . Ti- 11 alant 0.00 N/A 01/II/OS 07i10 :01 Vq a .l ./L iN 1.Op5 ~O~EIi1L1E .wlf I II Kad![I .d+ Nn I auneAtnq hemn 1 Netae TI+TUAo1d: 191 .0a tya M<. TArnhnldi 9N .ta npa ' !W . 7oeetM : 3 AT Ylndee : 70.0 6[l+sctld AT : 30 .{ etn slo . v1den3 0. :0 a .n . W~~' 0 .01 UP . teak Ratlee 5 .00 T+V " Adi " Nat1at 1 .00 Tae, Va1 . Ra1ot 7 .00 Va . RalatlV. AT: Ne I V"4 : e~y1i epMal TAON ~ LOON { .l+ ::::+ .i1lTen *+ .e+ I'I Axaeel.y:nv VI ac .cc Tiwi II 6M TbH Ne viwc N/A WA Y/A N/A an awca in sin s .o .1 i e.oDt1 ; LOp11 II $ a00N I 11 L(~ Laa1 IoDa 30.01 a EON 7 .OON 1.OON 1 !ON I 1d0a1 70000D ~ 0001 t3?. i' o ?~! . ~a! d?:.aa .eat rT .ee e.sD tOb,+o~_eo11 .~,;~t1 1uo,at,a Y 0 e 10 11 17 li f1 Printing Date: Friday, April 22, 2005 _ _ - 16~ie~ li to-itto ~ Figure 40 . Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . PFNA was not present (no peak at 10.6 min). p. 86 Company Sanitized . Does not contain TSCA CBI . Wildlrfe International, Ltd. -86- Project No.: 112E-108 , waw..a- "e-,eAer urw .q's+arvws.o . .r erwrn. " +,n.,w.toe.fsoMCr ssyls tneaa : 1" bwsYen.- swb 7yp.: n .n0 o.ao .y . . . .il, +ms GI<ulatNOaCenr: Y/A A<q. Gu : e"/71/OS . Mq . 71v, PTIOfOf .N w~mai,ino " r+rcen ie j YeUa TO[eMOle: If7J0 cpa IAaeaThroOele: q6S0 rpa !b . T.cetAat 1 '~ ' " [r"c,t" aM f ~I soP . nelnf '~ sap . nHaAra . uP " raas n.uel 30 .0 O .s ne 0 . :0 0 .01 s .eo UP : V.1 . "atlof Yae Yaatlv . Yi, ] .00 Ne ' 14[antieC7aw G w1YAtt Stul 71 :: ' u+a rl.af Ne N"Af N/A N/N NIA a rpnt .00, " ; rrral 7.OON I YfO.a I Y-04a , 0 .60N YY4ai f00Vai Loo,a 1 35b" I r ooaa : 2sd " j 1Y4a~ IS41 t04" 0000~0 000 _ ~{9L" 1 t 1 1 0 Prlnling Dale : Friday. April 22,2003 ~- , 11 Ol .g IT +SYT-~~ _ 7 ~0 f 10 11 13 ti~"11 1$ 10 " ~IY~1l~ " ~10 Figure 41 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . PFDA was not present (no peak at 11 .2 min) . p. 87 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -87- ~:na, rnrla_ t . 67V M : "017,06_OO.TIIAUE.~1"- Pa.tNa::w. "C.11aif YwyaaY~xTOR100aT1f ew,wat "la]f .,0.0WPA,olu7cr ,was[x "" ',~ aayl. rnan. Ir . sa.p~ent7.yavanon : .sla.noto .o a. ..n ,aoas, cawlataa cenn En eca. eaaa : o~nvos i Mw . Ttr, 07t10t01 PN ~I,l.i.`: .. . . .. . SLh .j IIMU7baaabla,~ ~ Nuw. Swaotwa: Nlnaw, ; " lapaetad "7t lsaavP". Nldtla Naonn aaP . reu naue : ' Eav . /"a7 . ,ualot 11 taP" Oal. Ytlel .e. .1.1- "T. 570 .1f 1 J0 .0 ILE O.iO o.at s.ao ..oo 7.eP . qa wln Ntmnm~n.a, NaIONn . tna 71we, No hat N/e N/A N/A_ c0a_a N/A wln Eqpa~ ae.0a 1 feea,~% 70DN<, ".7M1 SOWN ~ xso.al SOM.~ .WN "00,. ~ SSda~ SDOa ~ 20pa~ 1 , ue. l I ; ,PO ..Ieco:._ i ' i ~ i~ ' v 7 ~-b.lR ._-11 t~. tl~yyamtxspr r v t0 11 ,E 13 14 Is u ,i ,i -. .,o . . . . . .. ___ . . . . -~-. . ---~ . -~_ Printing Date : Friday, April 22,2005 . ._~.-~. ._ . . . . . ._-_ ._ . .._ . . . .._ . . .-- .---. '- '-. .' _ ..-. -- ----- Figure 42 . Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFUA was not present (no peak at 11 .8 min). Company Sanitized . Does not contpin TSCA CBI . Wildlfe International, Ltd. -88- Project No. ; 112E-108 WI-x'Idiifa xAtarMotienai.r Ltd . rc='Kg#2 kabser-::a'~. r~:a~:. :. '~p:e~~o:~ r~ t3it.s Aaciui~a,r.A : Y3lYl~ti1"41Ytt~txlt~: .t~~iJ.q N~?atai X*~~;qor~s'~.~~a.NnaT~~Az-r~A-1 ko .60zoa.o. i' D OW 1.1~ A~-JC ~07 3:'0'8 :G~'~3 :#~ x~ :3rT1liX> _ ~ti.~'1 Nur~e-~ o 3 ~UX024:03: 2:f1p:Q v s~r .~ r r v :y. uu uU t,~15~'~1 5 078, . ~ ~ ~r .-_. T~ - .>;x"" e^. ~t}o . ~zaQ. :n .~ .rf~l . .vv 494 ... . .7 a to ~+R~ . ' . .I~fA r. .~ ~.l~~ .V V ~ l'7 a : iQ'.Q: Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,g cartridge extract) from a Month 24 untreated control soil by GC/MS . 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -89- Project No. : 112E-108 " ;, .1 . _.. - . .. -~ . ., _ -. .-- . . .. -.. ..~.~.._.._..~..._.~._ Figure 44 . Representatide ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS. 8-2 FTOH was not present (no peak at 4.18 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -90- Project No.: 112E-108 , , a~ Figure 45 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS . 7-2 sFTOH was not present (no peak at 11 .9 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -91- Project No.: 112E-108 Ie.... 1 _T p u ~ a - b " ir-T~-j Figure 46 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace 8-2 FTA at 12 .3 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -92- Project No. : 112E-108 1 ..,.,. .N ::...,;..' , ......' . ,-s--r--- ., Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS . 7-3 Acid was not present (no peak at 11 .6 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -93- Project No. : 112E-108 -1, - - , .. P - --.T p h r~ Y i Figure 48 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LUMS/MS. 8-2 FTUA was not present (no peak at 12 .3 min) . Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. -94- Project No. : 112E-108 I -f Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . Trace PFOA at 9.91 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -95- Project No. : 112E-108 . , ,. ...i * I - I -----i~--- -r ~ i ~ A - I - .r . . i - n - : -- . - a- - A - a - n 1~ a Figure 50. Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . Trace PFNA at 10 .7 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife Intemational, Ltd. -96- Project No. : 112E-108 z. .: . .~:Y, .. ~ . . . . . . . . . . :, i Figure 51 . Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFDA at 11 .4 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -97- Project No.: 112E-108 Figure 52. Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LGMS/MS . Trace PFUA at 12 .0 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -98- Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\0801012 .D fjl 30 Apr 105 4 :29 pm GCMS#2 1719-TEX 8 Abundance Ion 31 .00 (30 .70 to 31 .70) : 0801012 .D Time-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 Ion 95 .00 (94 .70 to 95 .70) : 0801012 .D 1000 , 500- Time-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 Figure 53 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,e cartridge extract) from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10 .0 jug PFCAs/kg) control soil by GUMS . 8-2 FTOH was not present (no peak at 5.0 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -99- Project No.: 112E-108 .~.,. ~ 1 W ~C.:' . . ,. . ; `.-.' . . . . . S"a ~ `:E' .. , p .M P Ct ~~ N Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS. p. 100 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 2 p.:Nru~671lr~~aa~YT.W]i79:mf ~: I , sa .pc1 ...al..n~ea.,"tIne.loal,so9.7,weptKer Aawa9..- S~1. ~ c.elci:eiena io u9 +.l .il. t .mw' 'I cal .,aac .e cm<t -lnsu oy . .Lie Aa9 . >.edt GS IIAed . TSl .t O074t/]211t27 9N Medllladt 9. ~7lwtonanV r.ctcst 1 /; lol ., . Tns.anolat :4 .os cp. II Mu Tnnanola : lia .]l epa Ina. l~pOCns : 1 9T nnawt ]9 .0 ~) 9,--d IT, 12 .z :in ; .y. ~lacn : 0 .20 ' !.p" Il.Synst 0 .01 .00 ~lap. Adjk P-1 ., I .W ~ UaiA.l.tlwaRTt N.~ L.qppp~ 99oomf I ' 7000001 In6 7rp " t R.tmtlon T1 . .t Asut l.l9nct !uR Ta~al em ra .a : N .. Ta l.a. 17 .2 ~In 1907i . 19111.22I601~cpa .0 ~an 12 .1 eln i ~ 3609.00 I _ _ EMT.R ~' 0000 .00 I r"i aS0000I ~W000~ 110000 ~ MOO f ]E00.00' ;m99001 tMW 00000 :, Ow ~----1 ---f . ._ .~ -100- 12Z sw 102 98o 9_r91a.a 10r..,`, tay.tn 1' i]`-7s 75 ` 19 17 I 79-I Figure 55 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 jig 8-2 FTOH/kg and 10 .0 jig PFCAs/kg) control soil by LC/MS/MS . p. 101 Company Sanitized . Does not contain TSCA C81 . Wildlife International, Ltd. Project No. : 112E-108 -101- miri.:~: e _ .w In: 7 r: ' PnkNanr:'P-PIUY 64N("ty'4ST .MPP.Otmlf Camnl "K'17PE-tOP,7W,110{BPIKEY AmowPal :- ' a" api" Ind.at 1! aupl. Type, cent .ntutaen : OC 10,0 u9 100.E ~, Caleelabd Concl P .Jt7P u9 :a :.,t, :.//kkg9 . 4eq . Uae : 0" /21/OS ~ Ac9 . TSu : 07 :31137 PX do_ou-TE~wlr Ned111ed1 We Punchlnp Pactett 1 . Ne3a . Thtoholdl 201 .91 cpa ' A[ea Thteaheld: 1009.64 cpa AU. . Methe, 1 , R,. T. M11WeV : 30.0 .ap ct*d ATI 12 .3 ZX 0.20 :': -H.L.btl 0.01 cap. Peak Ratio: >.oo e .p . Ae . Aale: ".00 e .p . v+~ . Rate: 3.00 Ua" R"1Kt9 .AT: Ao 9.00.4 a3p.6 S QGa4 vw+ T i 'R ""t'mtYl"o'n T. .o : ArNI IN190t : itat TtIH : End Ttwl b"Tlt '.*l 0"u'ln 1t0610 . eoeeta 2696P .67307 cpa 11 .1 .kn 11 .3 an . 9eo.,1050.4 rr aso.~ i 300.4 1 ".Qa4 I 3,6~ .1 3 00.4 z50.6 : IifP z0o.. ; 11d1 E,R.91~a.W 1aoo j P .a lr-to,lTtu.u jueo na _ - -- a s 6-e37 atm~P ro n n 1~~=^Ri~ I 1a 1s 17 Is +o I _ , sa . . . ._ . . , . .~ .r.-.,-,. " .*.a,-e.~-..-~.----.- . . . ._~ . .. - m.an ... ----,u.. .- ,.- .J Figure 56. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 Etg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 102 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 nrolt unNt_ uro t .TITtTi:~ISISs_ao_TCI~Lt..~rt w.lwm- : "rFOncr utwhtl'11au~wD.m :r Owwn~NM," -II: X-100.10p,110(3RIfEj MwY6tn' "" Sstuw.1p9lnetT[yopee.x:7 1! x Cenwnl D.O u9 .4 ./L t00t91 . fa1w1 .tutleeG Cma 9 .1I70r v9 5,1 ./!9 My . Datt: 01/11/D! I~. Aca. Tlne: . 07 :7Lx7 PM .j xeeut .e, xe Suncn.n9 P.ctotl ! ! voiS . 74tttxald: 159.77 cpa MI. ThtuROld : 90 .14 rya 4 . ; W 4 : Ilu~ . a.aetW: 1 I IIT II1rWw : 30.0 ee Stqcc.d RT : 9.9r an S.D. vldtx : O.xe $ . " p Hi pht : OAI ~ Pxy . Put RtNol 5 .00 ~I txp . Ad7 . Yaclos 1.00 L PxP. Val. . . ._t=e. 7_00 SOh1' 75014- 700.. . I ~ 1 t T Ii Ret.ntlon Tlm: "at. To Na . 9.P1 .In ,IXl9ht : : It .tT sf .-1 . . 707Tl4469-cpa 9,67 -0 un 6.SON ~ ~'& S.OOtI . TTQii ~ sy 10.01 76G1~ 1 3.00.4 i TDON I J200N 1 1 .7Md 1 . 7I1/ON Q000.00 I OOD I- 1 ~7 -102- IIP "S 0 7 S .0 10 It-- 1777 le 41S~,1" 7bs!^h -7--l .`I= Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 Etg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS . p. 103 Company Sanitized, Does not contain TSCA CBI . Wildlife International, Ltd. -103- _ !w! ~ . ~~'' / Pw YN.~w ' "C"ASV W~b.2'Itf011, "O.k cw.w a ~ na .,a.1a. Dj " PaeT Arn!l. w banys. I,w.., Is !I aea::l::iien, soo w + .s.n Gicvl.aU canc : a~l wm DPl.r7aH/fes ua . .i ./z . Ac . Tim : 07 :)1l77 .n ".30.1 ~ aaC,faN, "'. : Fo : him I Noue .. . m2.1 91.!! cp . ; a.. . ~ x,.. mamle.i.iD...i,d . su.xs 1 .w I .a vlndw! l D. . ~D p.cbA Di, IO .f I~ LP " wlOta. D.DI a.j . M.\ ..tam 5.00 4e.PP " . b1t ". ".tioi a+a .: 1l. Vu 1 .1 ;+ n M. 1.70 LSD No :" soa ..n7t,renn: i, .., aaani, lU .a t,..: Gq Taw.~ "u. re u.e 1D .c .!n :1:"ucsase..PYa~i~P . ID .1 ID .e .(n " W.11 " M+1 a.OM4 i.l41 7.DDN 44M4 aaw~ !f0l1 600N ~ ISb17 ImN'I 7DON 3-- 1W.4 1~ 11A0+1 1 EDM WDD.DO Ow~ s~---- , a IOD " Project No.: 112E-108 Figure 58 . Representative ion chromatogram for PFNA in the soil extract from a Day 0 fortified (250 Rg 8-2 FTOH/kg and 10 .0 pg PFCAs/kg) control soil by LC/MS/MS . p. 104 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.; 112E-108 -104- .W.Ntin: _ t.0U1R'171" .h~~AtX~F- , p.ttN.m..'G10ArN 1lwtttF'61?dlfl0twf C.wwnl'117F-1M,Id.t1Ol7MIffY /wYHn- l .yL Inem U ~ fc~ua.0<1.aectt.7puam: ts0.0s .v . .1 ./t loots I eae.7a.a c.a<~ Rw . p asnav7sess uv -t .rtv ~i ura . n : pTOl :n vx I xeGltlcet XO Ywch1M r .c[on 1 yata. Taeuhste : Ia7J0 cPa Aa< Tluul .eld : Ol .so <p . I xca . Onnthn 1 N RT "1ndw : 1p.0 snp.<tN Rs . 17 .7 atn I aep . s .v . Nlt1<0ltonNe: Oo..toOl 7ap . r. .t aaa.. s.oo 90M<~ esa" : OOON ~ 7~ I i :p : v.~ : i:iia ioo '~ w. estn.. xr : xe 7oot< int. Typ. R.t.ntlm~tlq: Ivut N.t9Ma ftift ilo: pW iart 11 .ae 7e R... 11 .I da 1Tl!<e . Owta 7ltIbR7" O cp> 11 .0 xln 11 .c aan !. lRy! Y l0> " I # OooN iij/~ < 4M 100.c 130N 100N 7tON 7eM1 77Wt IOGN 500R00 G00 IIn .F1-,~,I 7 #~ III J21 6 R 7 ~ 01~0~It tt lf 1" 10 10 17 1 . 10 " Figure 59. Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 ~Lg 8-2 FTOHIkg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 105 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -105- ,,' tIlrn.:'C" 11 Add' Y[M(..k'St1N11..0 .m! II ~ CYwiWt'171f10.,t0 ..7701VIRY xWt .T." I' f+pls SM.x: IS e=:' .:<;oe. s o xa ..[ .ro 'iC .scxie[ .d Conc, +.suo~ +r ..s.rty Aw " ab, OVJI/Os Ii A[y. Tlaa 07 : )1177 )Y , oe~ t ~1 XMifNA : x .umntn7 be[en 1 Nela . 7A.n.M1a : 111 .0) aD . M.+ rhtoAold ; a70 .1! C.D tAU . axeetpa : I xr vlndoa: )o .o taD.cadtr, 1J . . .in .Dvletn : 1.n . wl~n<I oo..xmo ssxaDD ". rot Aef" X.cso, e+ese7 s.oo .wxD., eva,)s.l~.a.[.ser7. )ue.Nooo ans . rroal x.[.ec.ao rsw7 A[.at . XetpTt : ' St4[ 71w. W rlen 1+e. m .e.. )l . . ~ln 19 .p0. ants 71]1i. .1)N ry+ Il .i an 11 .0 .[n 0~ " .60H . Ioo . .e JsoH~ I J. oYw 56. 0. $ 6.OON =i a Yw ..roa. 17bt1 a OON ~ SYr .I _~ 1 1 [o..' Im..i 100000i 7 a t4 s o Met _ ....._.. ..,il~.nX[ ~ . ._ .. ~ . ... . .r Figure 60. Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (2501tg 8-2 FTOH/kg and 10.0 ltg PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -106- Project No. : 112E-108 Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : T :\DATA\ALFI025\0801012 .D DW 20 Apr 106 8 :09 pm aCMS#1 1735-TEX 112E-108,109,110 8 A-bundance Ion 31 .00 (30 .70 to 31 .70) : 0801012 .1) 2000 1000 Time-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 150 rime-->4 .00 4 .20 4 .40 T -r- F- 4 .60rT4 .80 5 .00 5 .20 5 .40 5 .60 Figure 61 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Month 12 fortified (250 Rg 8-2 FTOH/kg and 10 .0 ~Lg PFCAs/kg) control soil by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 107 - Project No. ; 112E-108 iqa~.i~ . ~ t ..=~jt 41 t i:itrG+a v-~~,t n ~t`~ t a t !w N wit 1 - Figure 62 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10 .0 Pg PFCAs/kg) control soil by LC/MS/MS . Trace 8-2 FTOH at 3.98 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -108- Project No.: 112E-108 a f~_ :6r .f: : wq . . m"a~ . ..CF. n. . .. . M ap uu q V u. ~im... rt . . us t .~~ Figure 63 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife Intanational, Ltd. - 109 - : `y'~iu. ..~n . . .. .. ., ;. ---- . '. . .~w :E~ .^.. . . .. Project No. : 112E-108 i > YWO Figure 64. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 110- Project No. : 112E-108 ....,^ - ez~.. .... . ,. : s Figure 65. Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (2501tg 8-2 FTOH/kg and 10.0 ltg PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -111- : Y~-i .i~ .uud "' "4 m.=111 ~ 41: A : . -------- ~einiw'1 ~ n " T IY Project No. : 112E-108 ---- Figure 66 . Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 jig 8-2 FTOH/kg and 10 .0 Ag PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. - 112 - Project No.: 112E-108 : E.- ml,2 Q= 1A, X n^.._* , , : E 4 Figure 67. Representative ion chromatogram for PFDA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 Etg PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -113- Project No.: 112E-108 Lryi iiipN 1 " q .n r 3 lu ~a1 q Figure 68 . . Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 ug 8-2 FTOH/kg and 10.0 Etg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 114 - Wildlife International, Ltd. GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Miec Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\1201020 .D fjl 30 Apr 105 7 :04 pm GCMS#2 1665-TEX 12 Pundance Ion 31 .00 (30 .70 to 31 .70) : 1201020 .1) 2000- 1000 ~ ITime-->4 . 00 undance 1500 -I 4 .20 4 .40 4 .60 Ion 95 .00 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 (94 .70 to 95 .70) : 1201020 .D 1000 500 Figure 69 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,g cartridge extract) from a Day 0 sterile soil treated with 200 mg/kg(, )by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -115- --~i: aae* .a s Project No.: 112E-108 Figure 70 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kgC )by LC/MS/MS . Trace 8-2 FTOH at 4.09 min was <LOD. p. 116 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. wttxun,: "wnr tr .y.p " nrmnouw" e.~cna ",Ix .,c"-" A " ~Ya[w.- s"apl .IM.a 70 s.npl . Try"t Unlnwn 4/A ,0a1 . bW xlst- .vol":" tt,uNxCa,t : 0 .0000 w/xvos u0 ".1 ./Y9 . MO " Tl..[ OY~lt:]l M Ie~Wwttnti7nWo[r" Pt: [7 b xoltt mrnn"]a: a.0. cPc A[u TIintAe]i: 370 .)1 pt v"Na 1 Ir, YloOOY : 70 .0 " ..t O17.7 PtP ...." .. .... 0.70 ftP " [w . YTnu~RU SUPi [rP" 1W7. Rtlloi 0.01 s.oo L00 U..etl " tiVe[.h %eO0 foo YO0.Y I "OCOW I rscm,ll fiNai ~I int. iieqrtlHt Mo N%AY Yn Arut i Ma"I,U I rmtT- AbpM! OOOOm- 6`n'Y, Ymm ; .oY.Y 7mnY 1700Y Iwom ICY.Y 16Y00 ,mYY YY Printing Date: Friday . April 22,200S . -116- t0Y Project No. : 112E-108 Figure 71. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg by LC/MS/MS. 8-2 FTA was not present (no peak at 12 .2 min) . p. 117 Company Sanitized. Does not contain TSCA CBI . Wildfe International, Ltd. - 117 - Project No. : 112E-108 j , " putllnnf"dOlur wWn)Y57 .OM.lO .wo' opmnrM'ltxe .lad-' Annorfar- danplt 1Mea : 20 aa.ptt Typtt VnkoOVn C-t c.ltulat"t'd IC"Z., D .oooo w . .l .nt Aoq, Drtn DtFlt/OS ' Acq . 7tw : Ofadal pN 9dOf.j ~Nodfl"d : NO wel00 RetOt7 1 11 Rots . 7Dttfheld : Aise TIRpsl .elA : 101 .93 10.9 .44 IV, cpt I; Nup, apeelhat 1 ~I Ri vi- )0.0 h50p9.ebd RT: 17 .1 n ' . Ntd111 N.p . ILfaRt : 0.20 0.01 R.p . sap. pAAdd."f1:.k At- %1- ~~ Cap . .L ttn : ~ VH MVl .tlVt FT : ~ .D9 1 .00 No 000.4 . dMs4 . . 80014 7 dON 70O4- II MCmO on,TU. : II Arul xflantl J ft.et Tbes :~ tnd Tln : s N/A N/A N/A N/A N/A twn owb ~tn ~ln I SON ~ 6.00s4 ~ f50N " 6.OOt1 : I fL. 1 SOrN ~ .00aj ,W I a oat I aso.. I 1 10.4 1 l.DOtt ~ dooD .oo , Printing Date : Friday, April 22,2005 daug 7.k 012 s. .lo.la nnsttid ta~ u.d3 o ,o 1, 19 0 14 "-ldid '~17 ~ ~4 -1o Figure 72. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg by LC/MS/MS . 8-2 FTUA was not present (no peak at 12.3 min). p. 118 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -118- !i- i.. . .'w:,- ~ w~arr- ~~t~~ea~ec~r,. c,~'tun..ov 4 ...r.a- 7.y1. ,od. .: 7e CMtlMifaroi "laX: aaetfn. .wlaad <eMl w.v . rlw: lfa..d,lrrhll ..dy :r.,Ke.c Yesa . ThruhvJd. ufa mraahad: ; M~ . fmelN. I MYt~Nm: I~ f.f "cl1eN.~ ' e.f " Yaaf4,. UP . e .af fatle. e.f . wal . .. ~X/A ef../uiul/fes w ..I .na ev.u~7t fn ! lff.n cpa fa .ff <f. I 711 .0 0 .20 "!n 0 .01 110. 1 .00 704f ILfOH SOW " eaw" " DOw i fON TaO .a ~ mr . r~al +; f.f.nclm r..a : I Ya1,1a11N' I' iM il .e : eaa. o f.n Y~n tuef . lea,f'o9layf. 10 .0 an " fo .f ~ "OON i 6" 1 nmw dqNr oow I amw aao.a I, 7SW i 10W I 114~{ f000.001 eml._ . . . l -_ _7 -7 ~~ 6 ~~ " . f &10 fi ~ iY U 4 - 7#- ,i * W, Figure 73 . Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg ( 1by LC/MS/MS . Trace PFOA at 9.81 min was <LOQ . p. 119 Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. -119- Project No. : 112E-108 S. .PL . tM.. . 20 5.W1 . TYP., U.I.- 1. N,A C.I. 1-d C n., 0. 0000 .9 . ., A9 .q. -, M19,31 W ... . .. . .. -Wdl ;-.h.1d . 96445 .,Z.3P :. M, ghti ..k R.".. Lap. M1 . X.tfsl L[D" V~1. X+bo[ V++ X.l.tfw "T: 30 . 10 .1 0 .2 . Ml S.00 60D 7.aD Xe :n`. Trpu mtlen Tfu : A[ "u N+SOht: itul TfNn ".M TtXe : Ne Owa\ X/A N/A N/A Y/A N/A s cps t aSn .In 1000~ 95M " m. ISMI 100M r w'l I 1.00H I "+DN% "OOH . SSMSOO+I +OON I'~ a00N . 7f0++ . 706" ~ 79M1~ 300N I 1 bOH ' [OM+ QOOOW~ 0DO ~ Printing Date : Friday, April 22, 2005 + ia i s ~ ---------- 7 i---`-d' 3M l.o.wrn n u ,o u -ie le v . . . _. .--------- . . _ . u 1, . _--- Figure 74, Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg( aby LC/MS/MS. PFNA was not present (no peak at 10.6 min). p. 120 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 120 - awa.l-YrwncY-e-rf~a-sa~rrwY-.~Yn~l-r0orforusw~cn-ar..ir:ai,aa TOY.rnrnt "ttab,D"" Mn.llfen.- a..p3 . 3fa.a: ie s.ro3 . rroa vntnoxn c.ieuitVt1.dqmcefm, d .xn/TAn xT ..f .a M"" D.Nf DI/il/OS Acy. Tlvt OMISf]I M f .Dp3' I, flONi wdl3Ndf ~I 31xn~lnf Iwwn ~ Yesa Thn"Mle: le 111 .30 tpt RvuThw.Reld, a7d .50 Jlu. awDUaf Rt YLdw: I up.faw arf a .Rp". 3donf wlynt : I i eTtt.o .. T..t sd3 . rtt3o . R.uw ,~ aust.D " wvtuau. vRe.ue.fn 3 7, .0 .a x .n D .3D D.D1 a.DO 4]..D0D xs II 3nt . ryn . . il aaentttn 23vf Rruf .1 Maaht: I,aotITW : end Tn .o T. aa. 11.2 ~In tlf3 .t 1336afDf9 xwtpl 11 .1 11 .7 fOG1l i eDD,I i rxw ~ i r.aow, e. tDyd i ~ a .aow j ; . m.. f .w. li .ao.. ~ aaw.: li 300" :~ i aDDJ am.l ~ LDDN i ,.fna + aDmfn' Printing Date: Frlday, April 22, 2005 . 'e ~ i -T~ib ' lu"-i~" lix'N 'tatan. 1Dia v 3i ' n ' Figure 75 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg~ >y LC/MS/MS. Trace PFDA at 11 .2 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -121- P*ShN-V-It~ C,1~1,t:d Con,, 0.01MO q P: 1 Wi th, . .0 : Z.ZP .- I:!OkhlR'Itto, PP : "V.1: "Mt" . : 0 .20 0 .0l S .00 'I" . ", T-, .. . . I . ... A~4 Z p. 121 ProjectNo .: 11213-108 3 6W4 3 OD.~ IN" :.OD.4 150.4 Printing ya~e~F ;iday, April 22,2005 Figure 76" Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg[ Iby LC/MS/MS. Trace PFUA at 11 .8 min was <LOD . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -122- Project No.: 112E-108 Wildlife International, Ltd . GC-MS#2 MSD Serial No . File : Operator : Date Acquired : Instrument : Sample Name : Misc info : Vial Number : T :\DATA\ALFI025\1301021 .D DW 20 Apr 106 GCMS#1 11 :09 pm 1682-TEX 112E-108 13 3118A02483 ~bundance, Ion 31 .00 (30 .70 to 31 .70) : 1301021 .D 30001 2000 1000 T'~ T ~Pime-->4 .00 4 .20 4 .40 T 4 .60 l T ~T T 4 . 80 5 . 00 ' 5 . 20 ' ' S . 4 0' 0 Abundance. Ion 95 .00 (94 .70 to 95 .')0)1301021 .D I 150 ime-->4 .00 4 .20 4 .40 4 .~60 4 .~80 5 .~00 5 .~20 5 .~40 5 .~60 Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Month 12 sterile soil treated with 200 mg/kgC )by GC/MS. Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -123- t. A;~` " "i ~w .._ . ,. . _ = Project No.: 112E-108 , 1 P ie , 1 94 : Figure 78 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kg(, I by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -124- Project No.: 112E-108 . ,d.:. ::"*. ,. .. .. . . .,. ws :.., ..., . a m ,-. S t 3 Figure 79 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgt 'by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -125- Project No.: 112E-108 =- - 177 ... . ,., . . W ,. Vl .. . , s * ; ; Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg( ))y LC/MS/MS. Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -126- Project No. : 112E-108 ~~. W: .,r,. .. 3 2I , r ar un ~moN i . . -. i . . . .ry_ ._ ..-7 Figure 81 . Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgL . ))y LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 127 - Project No.: 112E-108 b ., Figure 82 . Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg` ] by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -128- Project No. : 112E-108 .> . .~ ..~":3F, _ .~,~. . .. . a w ;u'R' .~ 1 ~.;k' . I Figure 83. Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg(- ,3by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wirdlrfe International, Ltd. -129- Project No.: 112E-108 ~l.a t4o ' .awi pP 4 i T- i ' ;- Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgt _) by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 130 - Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\1001014 .D fjl 30 Apr 105 5 :06 pm GCMS#2 1593-TEX 10 Abundance 3000 Ion 31 .00 (30 .70 to 31 .70) : 1001014 .1) 2000 1000- rr ime-->4 .00 GAbundance I , 15001 4 .20 4 .40 4 .60 Ion 95 .00 4 .80 5 .00 5 .~20 5 .40 5 .~60 5 .~80 (94 .70 to 95 .70) : 1001014 .D 1000 ~ 500 -, T ime--> 4 .00 4 .20 4 .40 4 .~60 4 .~80 5 .~00 5 .~20 5A0 5 .~60 5 .~80 Figure 85 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,g cartridge extract) from a Day O live soil treated with 200 mg/kk 3by GUMS . 8-2 FTOH is not present (no peak at 5.0 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 131 - Project No. : 112E-108 ;M.. ~fw r `:P': . 3 1 i~_ aa Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day O live soil treated with 200 mg/kg L jby LC/MS/MS. Trace 8-2 FTOH at 3 .93 min was <LOD. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -132- Project No. : 112E-108 'I:_ ..,- .... , ---, ----^ww--M~ ,, - -~ -s--~--z Figure 87 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day O live soil treated with 200 mg/kgt ))y LC/MS/MS . Trace 7-2 sFTOH at 11 .9 min was <LOQ . p. 133 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -133- r.[fw.r~aa L.tWe[Y'4?7 orMo0 -r Cpxn[n " 117F "tOV Mmblen~- faql[ tMtYx 17 Saqla 7yp.x Con<.nt[aaon: caleutaee cenc I hce. Wta ~ hcy. raM ; IY .Inam Y/A [ 0 01/21/05 00,14 .11 !w \ 00.[ 970000 Nmul.ex Yo ~ Eanehln9 rtebst 1 Nelu th[e[heldt 71 .06 qt lAtaa Thanheldx 120 .]t cp[ mu. YmpMa x 1 ~ Nr wlndaY : ]0 .0 Ij t[pPp .ebd Prx 12 .2 an Ntdth : 0 .]0 ~~ s .P . Y.cYhu e .ot C .p . Mak Y+tiei 5 .00 foooap OSW 00 f00pW Teaopo ~1 eq. . Va1 . IIKIex ' Uta blatfva AT ; ] .00 No 700000 ~rasntt..ntrarevn.: rt ..~ At.Pt uNsaylrcx rbei cneR .[tnrx Pa.. re t u.z ~ .in fr7 .ft ounts N] .Obt7 [ cpa .l1 utn 13 .4 0.500.00 t000m ' 060000 yII OOOD 00 E/ .50000 Impm xaum ]wo m 1l0000 ]pro.0p \500.00 tOmm 1 . . ... i _1T~ ] _ 1 ~~ . i ~_ 5 t0]I ~l7 ~-t~ 0 ._ . . . Is ~ t1 Tbn. Nn tz1] 1] 13 11 .. ~ 16 i~ ~ . 17 1! ~ 7p ..-.~~. ..a Figure 88. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day O live soil treated with 200 mg/kgC >y LC/MS/MS. Trace 8-2 FTA at 12 .2 min was <LOD. Company Sanitized . Does not contain TSCA CBI . Wildlzfe International, Ltd. -134- Project No.: 112E-108 ...., f I T Figure 89. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day O live soil treated with 200 mg/kgc` jby LC/MS/MS. 7-3 Acid is not present (no peak at 11 .6 min). p. 135 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -135- Project No. : 112E-108 I R no r"t.. . An ThC@ :h*9'4 I. .4t -ght, ".k NA.. UI..,t " .,I: Rali ; Ya . R .1K1w RTs cps tv" 12 .3 sin 0.20 0.01 5.0* 3.00 Yo lnt. 7ypa "e[enlfm Tlea~ An .: M.aaht : atatt Tue: ind 710: 'JO A/lAha 11/A N111 N/A N/A ntn . ep~t .ln wtn eaaaal em,a~ 1 7ww 7aba I l 400,4 ; ~ aaw ; ~ xaoH . i aao.a i am .1 I asow ~ 3O.D, ii I Zoo.i , , ap.a , ooaa i o owl . . i r S , ,o ttia ,i --16 ~ iev 1*8 - , -1* - - Figure 90. Representative ion chromato am for 8-2 FTUA in the soil extract from a Day O live soil treated with 200 mg/kg~ ~ ]by LC/MS/MS. 8-2 FTUA was not present (no peak at 12.3 min). p. 136 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 136 - sama .-~-'~an.Dla1V FO! W2106_ T I rCe.eIxNx.K~nr"t"nr]Er.o10e""c" rlwlmrwab.n.r.. -"al].a7no .nrr sop] . :al- n fup]. Typ .: VnYnnun Concmlrarlen : 0/4 1 OM ~ C11-11M Cenc : O .iSJ" S uq a .L/tq Atq . oata: 0~/7]/OS Mq" ttat 40a1 :]I tW OdM~O EUCAEW - I Eunentny Eaeten :~ xnla. 7nr .anetd: " Mea rnronolal x.ra. s..eena, PT WIMWt 1 Eay.eud "ti r lep. xfetn. Malqllt : ~ Ey. >u4 Ratla: EaD" adJ" Eatro: II E.D " WI :. ft-: I~ us.. nnlatlvr Rr 1 ]n.77 Oa .a 1 ]C.U 9.07 U.7U 0,41 5.00 a. W00 J. Ee epa qu Win I InA ` Ra.nSIlo- n h..t AfN7 s 81=u 17a , . : end rlu : .... te Oasa 0 .07 dn IUI7 " Cqxlll7 ]760 . 5q77 q 0 .70 oln0a la .o ~tn .OON E sora OOhll 7.lWa : r.00N 1 100a1i 007.a ~ e .ew j 600 .t~ a7aa : I OON ; ].aota,'/ ].OOra7/' iQ0.11 700r1 i 150.4 : t 00H 5= 00 f 000 1 - -'2 ' ]--%--- E " 7 ~ i -Se O -1- "t -I- 7 -" 1^ ] -" --- l"a-' - ;.--i's . Y7 S 10 Figure 91 . Representative ion chromatogram for PFOA in the soil extract from a Day O live soil treated with 200 mg/kgf _)by LC/MS/MS . Trace PFOA at 9.83 min was <LOQ . p. 137 Company Sanitized . Does not contain TSCA CBI . Wildlrfe International, Ltd. Project No.: 112E-108 - 137 - nvC'x:miTt6W_u . 1 Il~~Ct-x--'.n1.1. "2c%A -1~W Iw1eb6.n. ":,-aoillP.o . ..r ' s ..vt. I,w. . : l~l Aayl. TYpa VnYnam ConwOtfattent C .1w1 .tW Conm Ae1 . Dab : I Aeq . Tim, Y/A 0 .17996 u9 01/21106 06 :11 :19 PM ~.1 ./t0 100.696014 xodltl .dt Aa Pu1C~1p0 P.etor. I MOS. . tllrbpnlAl 792 .63 c9 . b .a TCteal..td : 964 . Z5 tOs Au . . s.eetba : i AT MM : CO..,te~AT . eM..vv.. rtatn: xaonr. ssAvtp,.. vut Ad] . A.tte: :j 6aP. Val . ~Mattll~oI: ' Vae Il.lat]Y. Pi: ]v .c . 10.6 ai e.lv o.ol s.00 Lvo ] .00 Ne 0~ J' A 60N e m.1 750N 700.1 lnts . ! A6te0ttTp0p0 U' Afaa . Mu01't : ',ft.a Tim : ; Lnd i-e . a. T. 9H1. " 10 .6 S6U .0 itln antl 084 .4i42 C Cp7 10 .1 16 .1 wan tld1~ 000 .1 i , " 6lb1 - - &~' 60M1 : EC 160N : " OON . ]9b11 I 3100m t 20W'/; 20DN1 .6b1- tmw= 7000 .00- 000 1 . " . 1 . 1--i --~7_--1---a~ 70 .11.7 ~~-1-2---1r0---.1-1 10 1! O 1~10 . -a :-+ .. ~:~ ~- . . . . ...~~ . . ._.""Y.. Figure 92 . Representative ion chromatoaram for PFNA in the soil extract from a Day O live soil treated with 200 mg/kg C --Iby LC/MS/MS . Trace PFNA at 10.6 min was <LOD . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. > , m. t~, ..:.. : x" .m.. .....~.*: ..., .m.. . i" . a, nr. rv u > . m:nm o.i ~i o una rm"> I ,w> i -138- Project No.: 112E-108 ,w>I 1m ..~ >mml , ee .> >aw ,mnm am , . i. ' 7-7, - i-- Figure 93 . Representative ion chromatogram for PFDA in the soil extract from a Day O live soil treated with 200 mg/kgG .]by LC/MS/MS . Trace PFDA at 11 .2 min was <LOQ . p. 139 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -139- NwP4N.nrta EwroN _ 7E . WYNtnw : "C .IIACIS WwIWaIqaitY " IIiE" 106" MnobEon :^ .OarnlCeiMIN' aa.p7. [M.at 17 f ..pl. typu VnYnwn t~- Cenuntrattom N/A c.tcutat.e cen. ;t Leq . Catu O .o0e0 uv t .t ./kq 01/ti/CO Acq . TL-, OttllelE PN NOdatitdt Ka Ev.Nlne facton 1 Nolan Thrtaholdt 151,1a .0IJS cpa Aru thr.aheldt cPa Nis . Eemthao 1 RT Nlndwt ]0.0 EaP.ctM RTt 11 .9 Rin SseePP.. ENladqtehtt : 9 .20 o .ol eRP . Pt.k Mtaoi 5 .00 Eae. 11dj . Rataat 1 .0000 caP. Val. Rattel 7. V Watt" Pit Xo 2-4 41,30" 0.00.4 ? .NN LOON ' 1- 7YPet I A At-Nt- T- rl NUql.h ' start Taatt !' EM 71rnt No i .ak N/A WN/AA N/A VIA K/A oYnt. cq Rin an + a00N ~ aaoN i eot. I 100.1 >~ 1 avwel aaa.~ L~.t 1.00.4 eooono ~I o.e' iTt i s i . -- tail tilaia Is~=--~i ir ' is lE Figure 94. Representative ion chromatogram for PFUA in the soil extract from a Day O live soil treated with 200 mg/kg( ]by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 140 - Project No. : 112E-108 wil.dlife .Inte;txVat:i,;o8a1, Ltd' . =^-Ni3.4$ MOD! $i~rial N.m . ~1~~ :t$~b~A113 ti~!e~strta~:a 6St~ h~te ~A~=Q.01x!et:i : 1a ~~ 107 ~fiM~lki, X1" zilP.t~3 : Y3.a!X W:t~te.,v : "7 3~i2 6 pa 1615-T-Ex. :11~~wI~:~ twa; 31 .0`.fJ (3.0 :7O t-C -3:3 ;7 . .) : *TO-1.011 15. 15fl~ }.-q 00 ~3 . :9:9 -Itrtia"-b ~ ' 4 . A'0 . ~` 4 4 -16 6. 4 : ~'07 Z &i~ ~ ~ . O:( . ~ !6i . ?Lil. . ~7-OW 1-5Q.' lJ~.~., , . v'V' Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8 cartridge extract) from a Month 24 1ive soil treated with 200 mg/kgC .]by GC/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 141 - p. 141 Project No.: 112E-108 .i . . . .r -~r.r .. .. .. - -`S Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg( ]by LC/MS/MS . Trace 8-2 FTOH at 3.96 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -142- Project No. : 112E-108 :as: ~aae .. ., ~- Figure 97. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live soil treated with 200 mg/kgC 3 by LC/MS/MS . Trace 7-2 sFTOH at 11 .9 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -143- ...., - Project No. : 112E-108 41. - . ._. Figure 98 . Representative ion chromatogram f r 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 mg/kgC ~by LC/MS/MS . Trace 8-2 FTA at 12.3 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -144- Project No.: 112E-108 .. i-- ., --s Figure 99 . Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live soil treated with 200 mg/kg~ jby LC/MS/MS . Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -145- Project No.: 112E-108 'o ,i".,1:..: .Tr.". :S'.'.r ..o.~..a ... .. .... S S d Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 mg/kfL ]by LC/MS/MS. Trace 8-2 FTUA at 12 .3 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -146- Project No. : 112E-105 J , ,. , .. , Figure 101 . Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil treated with 200 mg/kg[ Jby LC/MS/MS . Note : Secondary dilution required and reported for PFOA. calibration range. Original analysis was over Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. - 147 - Project No.: 112E-108 .~ .. ,...., - s : 3 - .,- , , Figure 102. Representative ion chromatoeram for PFNA in the soil extract from a Month 24 live soil treated with 200 mg/kgC - ~ by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -148- Project No,: 112E-108 . "...V - 1 - . - , ,. . . ,. Figure 103. Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kg(. ])y LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -149- Project No.: 112E-108 .., .... ' , .. . . , . . ---,------~ _ ,. , .. , , Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil treated with 200 mg/kgC ]by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. Project No.: 112E-108 -150Appendix I Measured Concentration of Selected Analytes (gg/kg Soil Dry Weight) Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -151 - Project No. : 112E-108 WiMfe Xnternatirmal, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (pg/kg) in Soils Treated with (' Soy 7Jype Ultisol . ' Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 1e Month 24 LOD =limit of detection (0 .206 pglkg) LOQ = limit ofquantitation (2.0 tigU Aerobic Live StaKe <LOQ <LOQ 17 .8 19 .1 32.0 36.5 57.8 61 .4 122 .1 150 .0 269.7 373 .8 376 .4 441.1 487.0 558,6 367.7 344.1 340.1 362 .6 410 .7 328 .7 365.9 329.1 <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ 2 .0 <LOQ 2.4 <LOQ 2.2 <LOQ 4 .9 <LOQ 7 .8 <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 21 .7 22 .7 26.8 23 .9 51 .5 40 .3 70 .0 73.3 114.7 133 .5 148 .6 104 .7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.5 <LOQ 2 .8 <LOQ 77iursday, November 08, 2007 Company Sanitized . Does not contain TSCA CB1 . Wildlife International, Ltd. -152- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (ILg/kg) in Soils Treated withC Soil Type Molliaol Sample Interval Day 0 Week 1 Week 2 week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD =limit of detection (0.206 #8V LOQ = limit of quantitation (2 .0 ply/kg) Aerobic Live Sterile <LOQ <LOQ 15 .4 15 .1 17 .8 18.0 21 .7 20 .2 31 .8 30.3 46.0 58 .6 74 .7 74 .4 92 .5 69 .3 103 .8 94 .0 98 .5 247.9 126.9 118 .8 229.3 207.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.9 <LOQ 3 .2 2 .3 3 .4 3 .3 3 .0 3 .1 Anaerobic Live sterile <LOQ <LOQ 14 .7 15 .1 18.8 18.1 20.2 22.3 42 .8 43 .2 78.5 61 .5 117.4 91 .0 . <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ 2,7 <LOQ 3 .6 2 .4 Tliursday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -153- Projcct No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA ( g/kg) in Soils Treated with t Soil Type Alfisol ' _ Sample -- Interval _ Day 0 Week 1 Week 2 Week 4 . Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = limit of detection (0.206 p8llcg) LOQ = limit of quantitation (2 .0 pg/kg) Aerobic Live Sterile <LOQ <LOQ 8 .5 8 .2 11 .4 11 .0 14 .9 15 .8 23 .2 25 .0 32.0 33.1 58.4 52.5 97 .9 93 .7 65 .4 84 .2 133 .1 103 .5 194 .4 155.9 196.5 190.7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 3 .3 <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ ' 9.4 10.9 14 .1 15 .4 19 .7 19 .8 17.9 31 .1 90.0 77 .8 99 .3 83 .5 <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <T.OQ <LOQ 2.0 <LOQ 2.2 <LOQ FYtday, October 11, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -154- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (Ag/kg) in Soils Treated witbr- Soil Type Incentisol Sample interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 . Month 12 Month 15 Month 18 Month 24 LOD =limit of detection (0 .206 Wg/kg) LOQ = limit of quantitation (2 .0 gglkg) Aerobic Live Sterile <LOQ <LOQ 18 .9 20 .8 28.6 29.0 55 .7 54 .7 122 .2 97 .7 190.4 189.4 264.7 250.4 288.5 339 .5 367.8 350.0 402 .3 385.3 422.5 446.5 530,9 554.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2 .1 <LOQ 2 .8 d.OQ 4.4 <LOQ 5 .6 3 .0 6.2 2.8 4.2 2 .5 Anaerobic Live Sterile <LOQ <LOQ 20 .9 17.7 26.5 26.8 75.5 48.1 138 .0 144 .3 284.5 302.6 327,0 347.1 Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 5 .0 <LOQ 7 .6 <LOQ Friday, OMObsr 17, 2007 p. 155 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -155- Project No. : 112E-108 WiTdlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FI'UA (Erg/kg) in Soils Treated with(L ] Soil Type Ullisol . Sample Interval Day 0 Week 7 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = limit of detection (0 .119 pg/kg) LOQ =limit of quantitation (2.0 pg/k$) Aerobic Live Sterile <LOD <LOQ <LOQ <LOQ 2 .1 2 .5 4.0 3.2 6.3 6.7 12 .3 12 .7 12 .1 19 .3 30.9 19.7 6 .0 7 .6 11 .3 13 .5 15 .6 6.4 15 .6 32 .3 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD ' Friday, October 19, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. -156- Project No.: 112E-108 Wildlife Xnternational, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 I'TUA (Fig/kg) in Soils Treated withc Soil Type Mollisol . Sample Interval Aerobic --- Live - Sterile - Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ 3.0 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q.OQ --WD <LOD Anaerobic Live -Sterile <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD LOD = limit of detection (0.119 pg/kg) LOQ=litnit ofquantitation (2 .0 pg/kg) Friday, oerober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -157- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (wg/kg) in Soils Treated witht Soil Type Alfisol . Sample Interval DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 . Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ Anaerobic Live Merile <LOD <LOD Q .OD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ 3.9 2.2 2.3 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD Q,OQ <LOQ LOD =limitof detection (0.119 pglkg) LOQ =limit of quantitation (2 .O Itg/1c8) Friday, Odobsr 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -158- Project No.: 112E-108 Wildlife International, Ltd, Project Number 112E-108 Measured Concentrations of 8-2 FTUA (ptg/kg) in Soils Treated with [ ] Soil Type Inccntisol , Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 ~ Aerobic Live Sterile <LOQ <LOQ <LOD Q.OD <LOQ <LOQ 5.9 5.0 25 .2 12 .3 77 .7 88 .9 164 .0 160.4 192.1 161 .0 209.5 206.9 225.6 252. 1 256.2 242.7 248,5 239.0 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 2 .7 <LOQ 2.2 <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOQ 7 .7 <LOQ 5.6 5.5 13 .0 13 .0 15 .4 10 .0 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD = Gmit of detection (0 .119 pglkg) LOQ = limit ofquantitation (2 .0 ltg/kg) Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -159- Project No . : 112E-108 Wildlife International, Ltd, Project Number 1121?-108 Measured Concentrations of 8-2 FTA (ug/kg) in Soils Treated with C Soil Type YJ1Hso1 Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic . Live Sterile <LOD <LOD <LOQ <LOQ 3 .8 3 .7 6.2 4 .7 8 .6 8 .5 9 .7 8 .0 10 .1 19 .0 19.7 12.0 3.0 4.4 4.3 3 .8 6.7 2.9 4.7 5 .2 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD 2 .0 Anaerobic Live Sterile <LOD <LOD <LOD <LOD -WD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <L0D <LOD Q.OI) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD LOD a limit ofdetecfion (0 .503 pglkg) LOQ =limit of quantita6on (2 .0 pg/kg) Friday, Oefober 11, 2007 Company Sanitized . Does not contain TSCA,CBI . Wildlife International, Ltd. -160- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (N.gJlcg) in Soils Treated with[ Soil7y'e . Mollisol Sample Interval Dayo Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live StertJe <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ 2 .3 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <AD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD=limit ofdetection~(0 .503 jig/kg) LOQ =limit of quantitation (2.O ItgAcg) Anaerobic .Give -Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Frlday, OCober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -161- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (pg/kg) in Soils Treated with e Soil Type Alfisol Sample Interval Day 0 week l Week 2 Week 4 , Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile _ _ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD QdD d.,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <1.OQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ LOD = limit of detection (0.503 ltg4cg) LOQ = limit of quantitation (2 .0 p&/kg) FrMoy, October rs 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 162- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (jig/kg) in Soils Treated with(- Soil Type-Ineentisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 ' Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = 13mit ofdetection (0 .503 ug/lcg) LOQ = limit ofquantitation (2 .0 Ng/kg) Aerobic Live Sterile <LOQ <LOQ <LOD <LOD <LOQ <LOQ 5 .5 4 .8 18 .0 13 .0 73.7 75.7 162.8 144 .6 163 .8 126 .8 133 .2 140.6 193 .0 205 .7 213 .7 226 .5 202.5 221 .7 <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 2 .9 <LOQ 2 .8 <LOQ <LO Q <LOQ Anaerobic Live bYerile <LOD <LOD <LOQ <LOD <LOD <LOD 6.6 <LOQ 2.5 2.6 6.1 5.9 8 .5 5 .3 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Friday, oaober 12, 2007 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -163- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA (pglkg) in Soils Treated withiL . Sample SollT,ppe-__--- -- Interval vltisot nay a . Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Lfve Sterile <LOQ <LOQ 7.0 7.2 11,5 12 .3 17 .6 16 .7 24 .0 21 .5 28 .0 30.0 28 .5 30.7 33 .2 30.1 8 .6 15 .7 23 .8 26 .2 29.8 24.4 31 .0 29.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ . 6.5 7.0 9.2 9 .5 15 .7 13 .7 15 .4 21 .5 23 .4 22 .4 30.0 25 .5 <1.OQ a.oQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =1'nnit ofdetection (0 .1241tg/kg) LOQ =limit ofquantitation (2.0 p8/kg) Friday, OMOber J2,2007 Company Sanitized . Does not contain TSCA CBI. Wrldlfe International, Ltd. -164- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Sail Type _ Mollisol Measured Concentrations of pFNA (gg/kg) in Soils Treated with I Sample Interi+al Aerobic Live Sterile Anaerobic Live Sterile Day 0 Weck 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 5 .8 6 .0 5 .7 8.3 10.6 12.3 18 .1 17 .2 22 .4 22 .5 21 .2 20,0 17 .3 11 .1 19.5 18 .4 14 .6 25 .8 20 .7 17 .1 25 .7 34.7 <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 4 .2 4 .3 5.8 4.6 4.3 5.9 6.5 8.2 12 .1 9 .3 16 .0 12 .1 <i.OD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ LOD = limit ofdetection (0.124 pg/kg) LOQ =limit ofquantitation (2 .0 pg/kg) Artaay, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -165- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA r9) in Soils Treated with C ! Soil Type Alfrsol Sample Inlerval Day 0 Week 1 Week 2 Wcek 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ 3.3 4.0 4,6 3 .9 6 .6 7 .7 10 .4 16 .2 10 .6 13 .5 8.9 15 .9 17.6 23 .3 13 .4 17 .5 23 .1 15 .2 15 .7 17.7 25 .3 22.0 <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ Q,OQ QAQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ 2 .4 2 .7 3.2 4.8 4.9 3 .6 6.6 8 .9 9.2 11 .2 7 .9 <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =linrit of detection (0 .124 ug/kg) LOQ =linut of quantitation (2.0 jig/kg) ~teoy, October 11 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -166- Project No. : 112E-108 Wildlife International, Ltd. Project Number 1121i 108 Measured Concentrations of PFNA (llg/kg) in Soils Treated withc 1 Soil Type Incentisol Sa-wle Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 ' Month 12 Month 15 Month 18 Month 24 LOD = limit of de2ootion(0 .124 pglkg) LOQ =1'vnitof quantitation (2 .0 ug/kg) Aerobic Live Sterile <LOQ <LOQ 20 .3 25.1 29,0 33 .4 36 .9 35.2 62,2 63 .9 59 .4 61 .9 67 .9 79.9 79 .9 79 .5 71 .5 70.7 73 .9 74 .1 79,2 80 .6 96 .5 104 .2 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 9.5 7.2 13 .1 13,5 34,9 21 .8 24 .8 26,6 28 .7 35,7 34 .0 36.1 <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ Friday, Odober 11, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -167- : Project No. : 112E-108 YN'Yldlfe International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA ug/icg) in Soils Treated withc Soil T~pe Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 . Month 18 Month 24 LOD = limit of detection (0 .114 pg/kg) LOQ = limit ofquantitation (2 .0 pg/kg) Aerobic Live Sterile <LOQ <LOQ 4.6 4 .9 7.6 8.4 10.7 11 .9 18 .0 18.5 26.6 28 .2 30 .4 35.5 37.9 39 .3 9 .2 20.9 33.3 34.0 48 .1 36 .5 50 .7 44.3 <I,OQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ ~<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 4.8 5 .2 6.9 7.3 9.3 8.5 13 .2 15 .0 25 .4 28 .4 21 .9 19.6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 77hur+day, Novnnber 0$ 2007 Company Sanltized . Does not contaln TSCA CBI . Wildlife International, Ltd. - 168 - Project No.: 112E-108 Wildltfe 1'nternational, Ltd: Project Number 112E-108 Measured Concentrations of PFDA (ug/kg) in Soils Treated withc Soll2ype Sample interval Aerobic Live Sterile Anaerobic Live Sterile Mollisol Day 0 Week 1 Wtek 2 Week 4 . Month 2 Month 4 Month 6 Month 9 Month 12 <LOQ <LOQ 3 .4 3 .7 4.7 5 .2 6 .2 6 .2 9.9 10.1 14.6 16.4 17 .3 17 .6 16:6 12 .4 19 .7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ 2 .0 <LOQ <LOQ <LOQ <LOQ 2.1 . <LOQ <LOQ <LOQ 3 .7 3 .7 5 .4 4.7 6.5 6.7 11 .0 10 .7 17 .4 14.2 21 .0 17 .5 <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ . <LOQ <LOQ <LOQ <LOQ Month 15 18.6 <LOQ 14.6 27.7 Month 18 22 .0 21 .4 Month 24 45.4 . 48 .5 LOD = limit of detection (0.114 pg/kg) LOQ a limit ofquantitation (2 .0 ug/kg) Thur.rday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 169 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (ltglkg) in Soils Treated with~ Soil Type Alfisol Sample Interval Day 0 Weck 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = limit of detection (0 .1141tglkg) LO Q = limit of quantitation (2.0 pglkg) Aerobic Live Sterile <LOQ <LOQ <LOQ 2 .1 2 .9 3 .0 4.4 4 .8 8 .1 9.2 8 .4 9 .8 9,3 12.5 17.1 19.7 12 .9 16 .1 26 .3 15 .3 23 .3 20 .7 37.6 36.8 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ q.,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ 2.4 3 .1 3 .6 5.4 5.6 4.8 8 .7 12 .9 13 .2 14.6 12 .2 <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <l.OQ <LOQ <LOQ <LOQ <LOQ <LOQ rriday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -170- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (pg/kg) in Soils Treated witlc Soil 7jrpe lncentisol Sanple Interval Day 0 Week 1 Week 2 Week4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = limit ofdetection (0.114 pg/kg) LOQ =limit of quantitatIon (2 .0 pg/k8) Aerobic Live Sterile Q,OQ <LOQ 4.5 5.4 8,1 8.3 15 .6 15 .6 34.3 29.8 43 .5 50 .4 52 .3 68.8 66.7 57 .2 62 .0 69.8 62,9 66,0 81 .4 77 .9 86.7 89 .6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.1 <LOQ 2 .6 <LOQ 3 .1 3 .1 2 .9 2 .3 2 .3 2 .0 Anaerobic Live Sterile <LOQ <LOQ 5 .3 4 .5 7 .5 7.8 20.9 11 .5 21 .4 22 .9 33.7 39.3 47.0 41 .0 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2 .7 <LOQ Friday, ocrobu 12, 2007 Company Sanitized . Does not contain TSCA CBI. Wrldl~fe International, Ltd. -171- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA glkg) in Soils Treated withC Sail Type Ultisol Sample ___Interval Day 0 Week 1 WWk2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = limit of detection (0.128 p.glkg) LOQ =limit ofquantitation (2 .0 pglkg) Aerobic Live Sterile <LOQ <1 .OQ <LOQ <LOQ 3 .0 3 .3 5.1 4.7 8 .5 8 .0 13 .2 15.0 16,0 16.4 18 .8 17 .0 3.6 10.5 15 .1 15 .5 19.2 16.1 22.5 22 .7 <LOQ <LOQ <LOQ <LOQ , <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <X.OQ <LOQ Q.OQ Q.OQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ 2 .7 2 .6 4 .1 3.9 6,9 8 .2 15 .5 13 .1 18 .2 17.6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October 11, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 172 - Project No.: 112E-108 Wrldlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA (pg/kg) in Soils Treated with I soil Type Mollisol Sample - Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month l2 Month 15 Month 18 Month 24 Aerobic Live SterAe <LOQ <LOQ <LOQ <LOQ <LOQ 2,1 2.7 2.7 6 .0 5 .7 10 .0 10 .8 9.5 9.6 8.2 4.6 8 .8 8 .6 6 .1 11 .6 10 .4 9 .2 14 .5 21 .3 <LOQ <LOQ <LOD <LOD <JOD <LOD <LOQ <LOQ <LOQ 4.OD Q.OQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live slerild <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 2.5 3 .5 6.0 4.7 6.8 5 .5 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD LOD = limit of detection (0.128 ILglkg) LOQ = limit of quantitation (2.0 wglkg) Friday, odob.r ts, aoo7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -173- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA ~g/kg) in Soils Treated withL Soil Type Atfisot Sarnple Interval Dap 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOQ GLOQ <LOQ <LOQ 2 .0 2 .3 4 .6 6 .1 4 .1 6 .2 3 .2 6 .2 7.3 11.3 7.6 9.2 13.5 5.8 7.0 7.2 14.4 10.1 <LOD <.OD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOQ <.OQ <LOD <LOQ <LOQ Anaerobic Live Sterile <.OD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 3 .0 3 .1 3.3 4.5 3.5 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD Q .OD <LOD <LOQ <Y .OQ LOD m limit of detection (0 .128 pg/kg) LOQ a limit of quantitation (2.0 p&/kg) Friday, October la, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 174 - Project No.: 112E-108 Wildlife 1'nternational, Ltd. Project Number 112E-108 Measured Concentrations of PFUA (w,g/kg) in Soils Treated witb )C Sam,ple Soll Type -- - Interval Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ <LOQ 2.4 3.9 4.3 7.1 6.0 19.0 19 .8 18 .7 18,8 20 .8 28 .0 29 .4 28 .2 25.9 29.3 26.1 25.7 34.9 33 .0 40.5 45 .3 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOD <LOD <LOQ <LOQ 3 .4 3 .5 9 .6 6 .3 14 .1 14 .2 17 .1 19 .1 22 .2 21 .1 <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ LOD =limit of detection (0.128 Ag/kg) LOQ = limit of quantitation (2 .0 pg/kg) Friday, October 11, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -175- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p.g/kg) in Soils Treated with r Soil T,'y Ulttsol Sarple Interval Day 0 Week 1 Weok2 Woek 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterlle <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOD QAD <L.OD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (22 .3 Nglkg) LOQ = limit of quantitation (100 pgU atoteday, October ls, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -176- Project No. : 112E-108 Wildlife International, Ltd. . ProjectNumber 112E-108 Measured Concentrations of 8-2 FTOH in Soils Treated withc r) Sample Soil T-y--p-e--------- --I-nt-e-r-v-al----- - Aerobic Live Sterile Mollisol ' Day 0 Week 1 Weck2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 1_2 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limitof detection (22.3 uglkg) LOQ =limitofquantitation (100 W8/lc8) Monday, October 15, 2007 Company Sanitized. Does not contain TSCA C81 . Wildlife InteYnational, Ltd. -177- Project No.: 112E-108 . Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH ()jLg/kg) in Soils Treated withc 4 Soil Type Alfisol SamPle Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterlle <LOD <LOD <LOD <LOD <LOD <LOD <LOD <I,OrI) <LOD <L0D <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 4.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (22 .3 WW LO.Q = limit of quantitation (100 pg/kg) Monday, octob.r is, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 178 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (W./kg) in Soils Treated with (- Soi171~pe.. . Sample Interval Aerobic Live Sterile Incentisot Day 0 Week l Weck 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD Q.OD <LOQ " <.OQ 174 125 148 138 <LOQ 140 100 <LOQ 131 165 173 121 <j,OQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (22.3 Nglkg) LOQ = limit of quantitation (100 pglkg) An4erobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -179- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (itg/L) in Headspaces Gases from Chambers Treated with~ Soil Tppe _ Sample Interval Aerobic Live Sterile Ultisol ' Day 0 Week l Waok2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <1,OQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (12 .5 pg/L of headspace) Anaerobic Live Sterile <LOQ <L0Q <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Company Sanitized . Does not contain TSCA C81 . Wirdlfe International, Ltd. - 180 - Project No.; 112E-108 Wildlife International, Ltd. - Project Number 112E-10a Measured Concentrations of S-2 FTOH (Itg/L) in Headspaces Gases from Chambers Treated with[ Soil Type_ _ - Sample Interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 Wcck 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (12 .5 pglL of headspace) Anaerobic Live Sterile Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, Octobn 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -181- Project No.: 112E-108 Wildlife International, Ltd. Project Number 1128-108 Measured Concentrations of 8-2 FTOH (1%glL) in Headspaces Gases ,From Chambers Treated with C Soil Type Sample Interval Aerobic Live Sterile Alfisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LO Q <LOQ LOQ =limit of quantitation (12 .5 pg/L of headnpace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October lS 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -182- Project No. : 112E-108 Wildlfe International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p.g/L) in Headspaces Gases from Chambers Treated with C Sample Interval Aerobic Live sterile lncentiso! Day 0 Week 1 Week 2 Week 4 Month 2 Mouth 4 Month 6 Month 9 Month 12 Month 15 Month ls Month 24 .< .OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ Q,OQ <LOQ <LOQ Q.OQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ=limit ofquantitation (12.5 Ng/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ tI,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October ts 2007 p. 183 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -183- Project No. : 112E-108 Wildlife International, Ltd, Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (jig/kg) in Soils Treated with[- Soy Type Ultisol Sainple _ Interval Day 0 , Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ 133.0 166.1 <LOQ <LOQ <LOQ <LOQ 115 .6 <LOQ <LOQ <LOQ <LOQ Anaerobic Live merik LOQ =limit of quantitatIon (l00 pg/kg) 7Trursday, Novimber 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -184- Project No.: 112E-108 Wildlife International, Ltd. Project Number l 12E-108 Measured Concentrations of 7-2 s FTOH (jig/kg) in Soils Treated withC Soil Type Mollisol Sample Interval Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 month is Month 24 Aerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile LOQ = limit of quantitation (100 pg/kg) Thursday, November 08, 2007 Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. -185- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (wglkg) in Soils Treated withc, SoiiType - AlGsol Sampie Intervat Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month LB Month 24 Aerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <j,OQ <LOQ Anaerobic Live Sterdie LOQ - limit of quandtation (100 pg/!cg) Thursday, Navember 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 186 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (jtg/kg) in Soils Treated with( Sample Soil Type--- _ Interpal Incentisol Day o Month 2 ' Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ Q.OQ <LOQ Q.OQ 278 .3 278.3 192.8 464.2 193 .0 118.9 173 .2 182.2 <LOQ Q,OQ <LOQ <LOQ LOQ = limit of quantitat3on (100 gg/k8) Anaerobic Live Sterile 2wu.aday, November oa, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -187- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (jig/kg) in Soils Treated wit11C Soil e Ultisol Sample Interval Day 0 Month 2 Month 6 Month 9 ' Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ Q.OQ 6.6 9.1 16 .3 21 .4 31 .3 28.7 41 .7 33 .4 34.1 40.1 27.8 17.0 54.3 56.2 Anaerobic Live Sterile LOQ = limit of quantitation (2.0 ttg/lcg) Wednesday, March 17, 2008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -188- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Sell Type Measured Concentrations of 7-3 Acid (}Ig/kg) in Soils Treated with L Sample Interval Aerobic Live Sterile Anaerobic Live Sterile Mollisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 2.4 2.7 2.7 2.9 5 .0 4.8 6.6 9 .0 3 .5 3.4 8 .9 6 .6 LOQ - linnit of quanlitation (2 .0 ug(kg) Wednesday, Mereh 12,20#8 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -189- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 So117ype Alflsol Measured Concentrations of 7-3 Acid in Soils Treated withC T Sanple Interuai Aerobic Live Sterile Anaerobic Live Sterile Day o Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q,OQ <LOQ <LOQ Q.OQ 3 .2 2 .5 3 .2 2 .5 4 .9 6 .1 7 .6 8 .9 8 .1 6 .4 6 .6 7 .3 LOQ= ltmit ofqusntitadon (2.0 ltg/kg) Wednesday, March 12,29#8 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -190- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid ()Lg/kg) in Soils Treated w9thc Soti Ty,pe Inceatisol ' SamPJe Interval Day 0 Month 2 Month 6 Month 9 Month 12 Month is Month 18 Month 24 Aerobk Live Sterlle <LOQ Q,OQ 16 .7 9.6 67 .7 81 .6 128.3 153 .2 277.0 246.2 260.7 289.7 282.3 212.2 355.2 321.9 LOQ - limit of quantitation (2 .0 Wg/lcg) ~4naerobic Live SterVe Wedneaaay, March 12, 2008 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -191- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (gg/kg) in Untreated and Fortified Soils Soll Type - Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week i Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ ' LOD =limit of detection (0.20611g/kg) LOQ = limit of quantita.tion (2.0 tlglkg) Forti}led Recoveries Aerobic Anaerobic 9.5 93% 7.8 78% 8.6 86% 9.1 91% 9.2 92% 9.2 92% 10 .4 104% 10.3 103% 12 .0 120% 11 .4 114% 10.6 106% 10.4 10496 9.2 92% 10.0 100% 7.4 74% 6.8 sa9c 9 .5 9.2 10 .5 11 .3 9.2 9.8 9.3 9.0 11 .4 10.8 11 .4 11 .3 11 .2 11 .5 95% 92% 105% lu% 920.4 9t% 93% 90% 114% los% 114% 113% 112% 115% Friday, October 11, 2007 Page l oJ4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -192- Project No.: 112E-108 Wildlife International, Ltd. project Numbers 112E-108,109,110 Measured Concentrations of PFOA (pg/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 4.OD <LOD LOD = limit ofdetection (0.206 pg/kg) i.oQ =ltnlitofp,lantiration (2.0 ug/kg) Forfifled Recoveries Aerobic Anaerobic 8 .9 9 .3 9,7 10 .0 8.8 9.4 9.1 9.0 10.7 11 .2 13 .2 12.1 13 .1 12 .0 11 .4 11 .6 9 .2 10 .4 8915 93% 97% loo% aa% 94% 91% 90% 107% 112% 132% 121% 131% 120% 114% 116% 104% 9.3 93% 10.1 101% 8.5 85% 9.6 96% 8.5 as% 8.9 a9% 9.5 95% 9.9 9s% 12.2 122% 10.9 109% 14 .0 140% 13 .9 139% 14 .5 145% 14 .3 143% Friday, October 12,2007 page 2 ofa Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 193 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (Etg/kg) in Untreated and Fortified Soils soil Type Sample Interval Untreated Controls Aerobtc Anaerobfc Alflsol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOQ d.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.206 pglkg) LOQ =limit of quantitation (1.0 kg/kg) Fortified Recoveries Aerobic Anaerobic 9.2 92% 8.3 83% 8 .9 99% 10 .0 loo% 9 .9 99l6 9 .4 94% 10 .0 100% 10 .1 loi% 11 .0 uo% 10 .7 107% L0 .8 108% 11 .5 115% 11 .0 110% 11 .1 lulx 11 .5 usx 12 .5 125% 9.2 92% 9.3 93% 9.2 92% 8.2 sz% 8.7 sr% 9.3 93% 9.6 96% 10 .0 loo% 10 .6 106% 10 .4 104% 10 .4 104% 10.7 107% 11.1 111% 10.9 10914 10.5 105% 11.2 112% Priday, October 12, 20#7 Page 3 ojI p. 194 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -194- Proj ect No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (p.g/kg) in Untreated and Fortified Soils Soil Type-- Inceutisol Sanple Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L.OQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ FortlJ7ed Recoveries Aerobic Anaerobic 10 .4 10 .5 12 .9 10 .1 10 .6 10 .9 12 .4 11 .2 11 .3 11 .7 13 .4 10 .8 12 .1 10 .6 14 .5 12 .4 9.6 9.6 104u. 103% 129% 101% 106% 109% 124% us% 113% 117% 134% 108% 121% 106% 14s% 124% 96% 96% 9.7 97% 9.4 94% 10 .9 109% 9.8 98% 10 .8 los% 10 .5 105% 10 .7 107% 10 .9 109% 11 .9 119% 11.1 111% 10.6 106% 11 .5 115% 11.3 113% 11.6 116% LOA =limit ofdetection (0 .20611g1kg) LOQ = limitofquantitation (2.O llgflcg) Friday, October 12, 2007 Page 4 of 4 p. 195 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -195- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (Ftg/kg) in Untreated and Fortified Soils Soil Type _ Ultisol ' Sanple Interval Day 0 Week i Week 2 Week 4 Mouth 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD Fortifled Recoveries Aerobic Anaerobic 7.2 72% 6.5 65% 7.7 77% 7 .9 79% 6 .9 69% 6 .9 69% 7.2 72% 7.2 72% 8.9 89% 8.0 80% 6.5 6556 7.3 73% 2.9 29% 5.4 54% 5.1 51% 4.0 40% 8.0 90% 7.4 74% 7.7 77% 8.7 87% 8.4 84% 8.2 92% 8.2 sz% 7.9 79% 9.3 93% 7.6 76% 8,2 snc 8.0 80% 6.5 65% 7.0 70% LOD -lnnit of detection (0.119 pg/kg) LOQ =limit of quantitation (2 .0 pg/kg) Friday, Oetabrr 1$1007 Page I oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 196 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (Itg/kg) in Untreated and Fortified Soils Soil Type Mollisol , Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD LOD=limit o detection (0,11911g/kg) LOQ = limit of qusntitaticel (2 .0 pg/kg) Fortifted Recoveries Aerobic Anaerobic 9 .2 9 .0 8 .1 8.5 9.1 9.5 9 .0 9 .1 8 .8 8.7 10.0 9.4 10.2 8 .2 7 .8 9 .0 5 .9 6.3 92% 90% BI% 85% 91% 95% 90% 91% 88% 97% 100% 94% 102% 22% 79% 90% 5rA 6346 7.3 8 .8 7 .7 8 .7 7.7 9.8 8 .0 8.0 9.7 7.8 9 .9 10 .7 10,0 8.5 rt% 88% 77% 87% 77% 98% ao% 80% 97% 7e% 99% 107% loe% 95% Friday, Ouober 72, 2007 Page 2 ofC Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 197 - Project No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (gg/kg) in Untreated and Fortified Soils Sample Soil Type ---,interval Al6so1 Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <.OD <LOD <LOD <LOD <.OD <LOD Fortifled Recoveries Aerobic Anaerobic 8.4 7.7 8.6 9.8 10 .5 7 .6 7 .5 9 .2 9.6 9.1 6 .6 9 .0 8 .4 9 .0 8.0 11 .1 6.2 6 .7 84% 77% 86% 98% 105% 76% 75% 92% 96% 91% 66% 90% 94% 90% 90% 111% 62% 67% 8 .9 7 .5 9 .3 9 .5 9.2 8.9 8.6 9.4 10.9 10 .8 8 .1 8 .3 10.3 8.3 99% 75% 93% 95% nx 89% as% 94% 109% los% 81% 83% 103% 83% LOD = limit of detection (0.11911g/kg) LOQ = limito quantitation (2 .O Ilg/kg) Friday, October II, 2047 Page 3 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -198- Project No. : 112E-108 Wrldlffe International, Ltd. ProjeetNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (pg/lcg) in Untreated and Fortified Soils Soll Type Tncentisol Sarnple Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <i..OD <LOD <LOD <LOD <LOD LOD = limit of detection (0,119 pglkg) LOQ = limit of quantitation (2 .0 pg/kg) V Fortified Recoveries Aerobic Anaerobic 11 .0 9.6 10 .8 8.3 8 .8 9 .7 9 .4 7 .9 8 .0 8.9 10.1 8 .1 7.0 6 .8 7 .4 6 .4 5.3 5 .2 llo% 9676 los% 93% 88% 97% 94% 79% 80% 89% 101% sl% 70% 68% 74% 64%' 53% 51% 7.9 79% 8.1 81% 8.3 83% 7.5 75% 8.5 85% 7.8 78% 7.7 77% 8.2 82% 7.6 76% . 8.8 88% 6.6 66% 7.0 70% 6.1 61% 7.1 71% Friday, October 12, 2007 p Company Sanitized . Does not contain TSCA CBI . Wrldlrfe International, Ltd. -199- Project No.: 112E-108 Waldlfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (Wg/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample Interval Day 0 Weak 1 Weak 2 Wcek4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 .503 Ng/kg) LOQ = limitofquantitation (2.0 llg/kg) Values in parentheses are <LOQ and uLOD Fortyled Recoveries Aerobic Anaerobic 6.1 61% 5.8 58% 6.2 62% 6.9 69% 5.4 54% 5.5 35% 5.4 54% 5.3 53% 3.3 33% 2.4 24% 3.4 34% 3.7 37% (1 .4) (1 .4) 4.1 41% (1 .7) 6.7 67% 6.6 66% 7.0 70% 6.9 69% 6.1 61% 6.5 65% 5.3 53% 6.3 63% 7.2 72St 5.1 51% 4.8 48% 4.6 46% 3.5 35% 3.2 321% Friday, Ouobsi 11, 2007 Page I of 4 Company Sanitized . Does not contain TSCA CBI . Wrldlfe International, Ltd. - 200 - Project No.: 112E-108 Wirdlfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (Wg/kg) in Untreated and Fortified Soils Soil Type --- -Mollisol . Sanrple Inter_val- -Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD 2 .7 <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 .5031tp4W LOQ = limitof quantitation (2 .0 pg/kg) Values in parentheses are <LOQ and >=LOD Fortoed Recoverles Aerobic ~ Anaerobic 8.7 67% 9.0 90% 8.4 8414 9.0 90% 9.0 90% 8.8 88% 8.3 93% 8.1 ei% 7.8 78% 7.9 79% 8.4 94% 6.9 sv% 6.4 64% 6.2 62% 5.0 3o% 6.2 62% 2.3 23% (1 .7) 7.0 70% 8.7 87% 7.1 71% 7.6 76% 6.4 64% 8.3 83% 6.3 63% 7.0 70% 8.0 ao% 7.1 71% 5.8 58% 6.5 65% 5.7 57% 5.2 s2% Friday, October 11, 2007 Page 2 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -201- Project No .: 112E-108 Wildlife International, Ltd. project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (ug/kg) in Untreated and Fortified Soils Soil Type Affisol Sanrple Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 4,OD <LOD <LOQ <LOQ LOD - limit of detection (0 .503 pg/kg) LOQ = limit of quantitafloa (2 .0 ylg/kg) Values in parentheses are <LOQ and >=LOD FonVedRecoverles Aerobic Anaerobic 7.8 78% 7.9 7916 7.8 78% 8.4 aa% 10,6 106% 8.7 s7% 6.8 68% 8.4 94% 7.5 75% 7.0 70% 4.2 42% 6.7 67% 6.0 60% 6.6 66% 5.1 51% 7.7 77% 4.0 40% 3.7 37% 8.8 88% 6.7 67% 8.5 85% 8.4 84% 8.4 84% 8.1 81% 7.7 77% 8.3 93% 8.8 sa% 8.3 83% 6.7 67% 6.6 66% 7,3 73% 7.0 769i Friday, Ootobar 19, 2007 Page 3 of 4 Company Sanitized. Does not contain 7SCA CBI. Wildlfe International, Ltd. - 202 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of S-2 FTA (Itg/kg) in Untreated and Fortified Soils Soil Type Incentisot Sample Interval Day 0 Week 1 Weak 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <J,OD Q,OD QOD <LOD Q,OD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD LOD =lanit of detection (0.503 pg/kg) LOQ = lirnit ofquantitation (2 .O ItgAcB) Values in parentheses are Q.OQ and >=LOD Fortlj7ed Recoverks Aerobic Anaerobic 10.7 9.6 9 .8 7.6 7.0 8 .4 7.1 6.3 5 .5 5 .8 5 .3 4.5 4.3 3 .7 3 .4 3 .4 2 .1 (1 .8) 107% 96% 99% 76% 70% 84% 71% 63% ss% st% 33% 45% 431A 37% 34% 34% 21% 7.8 78% 7.2 72% 7.0 70% 6.7 67% 7,2 n% 7.6 76% 5.9 5996 6.6 66sa 4.8 43% 6.3 63% 3.6 36% 3.4 34% 2.6 26% 3.5 35% Friday, October 1?, 2007 Page 4 ofJ Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. - 203 - Project No. ; 112E-108 Wildlife International, Ltd, Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (Itg/kg) in Untreated and Fortified Soils soil 7~pe _ Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit of detection (0 .12411g/Jcg) LOQ =limit of yuantitation (2 .0 pg/kg) Forfij7ed Recoveries Aerobic Anaerobtic 9 .4 7 .8 9.7 9.8 9 .5 9 .8 10 .4 10.1 11 .4 11 .4 10.3 10 .6 7 .0 10 .3 7.9 6.7 94% 78% 97% 98% 95% 99% 1006 101% 114% 114% 303% 1o6% 70% 103% 79% 67% 10 .0 9.9 9.9 10 .7 9 .7 10 .3 10,3 9.6 11 .2 11 .0 11 .6 11.7 10.8 11 .6 loo% 9996 99% 107% 97% 303% 103% 96% lls% 110% 116% 117% l0t% 11696 Friday, October 1$ 2007 Page j of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -204- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (gg/kg) in Untreated and Fortified Soils Soil Type Mallisol Sample Interval Day 0 Week 1 Week 2 Wock 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month le Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <.OD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD a limit of detection (0.124 pg/kg) LOQ= limit of quantitation (2 .0 Ng/kg) Fortified Recoveries Aerobic Anaerobic 9.4 9.6 9.7 9.7 9 .5 9 .9 10.2 8.6 10.7 10.6 12 .2 11 .9 12.0 11 .7 10 .0 11 .2 8.1 8 .3 94% 96% 97% 97% 95% 99% loass 86% 107% 106% 122% 119% 120% 117% 100% 119% 91% 8386 9.6 10 .1 8 .9 9 .8 9.6 9.9 9 .3 9 .8 10 .7 10.1 11 .6 12.8 12 .2 11 .9 96% 101% 89% 98% 96% 99% 93% 98% 107% lolx 116% 129% 122% 119% Friday, Odobe 12, 2007 Page? 0j4 p. 205 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 205 - Project No. : 112E-108 Wildlfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (pg/kg) in Untreated and Fortified Soils Soil Type Atrisol Sample Interval Day 0 Week I Week 2 Weck 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month I8 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOQ <LOQ <LOQ <LOQ <1,OQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ Fortifed Recoverles Aerobic Anaerobic 9.8 9 .3 9 .4 11 .2 10 .3 9.1 10.3 10.7 11 .3 11 .0 10.0 11 .4 10 .8 11 .4 11 .3 12.3 8.7 8.6 98% 93% 94% 112% ]03% 91% 103% 107% 113% 110% 100% 114% los% 114% 113% 123% 97% S69i 9.7 8 .1 9.4 10.1 9.8 10.1 10 .3 10 .0 11 .0 11 .0 10 .7 10.7 11 .5 10 .2 97% 81% 94% 101% 98% lol% 103% loo% 110% 110% 107% ]mu 115% lo2x LOD =limit ofdetection (0.12411p/kg) LOQ = limit of quantitation (2 .O Ilglkg) Friday, October !$ 2007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBI . Wildrife International, Ltd. -206- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (itg/kg) in Untreated and Fortified Soils Soil Type Inceotisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 month 12 Month 13 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ 4..OD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOD Q,OQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ Q,OD <LOQ . <LOQ <LOQ <LOQ Fortified Recoveries Aerobic Anaerobic 12 .4 11 .6 13 .0 10 .6 10 .7 10.7 12.5 10.7 11 .2 11 .9 14 .0 10.5 10.4 10.0 13 .0 10 .9 8 .9 8 .8 124% 116% 130% 106% 107% 107% 125% 107% 112% 119% 140% los% 104% loo% 130% 309% 0% 88% 10 .2 10 .0 10.8 9.8 10.3 10 .2 10 .1 10 .7 11 .1 11 .1 11 .0 10.8 10 .1 10 .8 102% 100% 108% 98% 103% 102% 101% 107% 111% Ill% lio% los% 101% los% LOD =lilnitofdetection (0.124 kg/kg) LOQ =limit of quantitation (2 .0 tlg/kg) FlJday, October 11, 2007 Page 4 0/4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -207- Project No.: 112E-108 Wildlfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils Sample So~--- Interval Ultisol Day 0 Weak I Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 4.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit ofdetection (0.114 Ng/kg) LOQ = limit of quantitation (2 .0 pg/kg) Fordjted Recoveries Aerobic Anaerobic 9.3 93% 7.4 74% 8.9 89% 8.6 86% 8.8 88% 8.9 s9% 9.4 94% 9.3 93% 10.9 309% 10.9 109% 9.6 96% 10 .0 100% 5.2 52% 9.5 95% 8.4 84% 7.0 oo5s 9.2 9.1 9.0 9.7 9,4 9.2 9 .3 8 .0 10 .4 10 .2 11 .0 10.9 9.6 10.4 92% 91% 90% 97% 94% 9245 9316 s6% 104% 102% 110% 109% 96% 104% F}idny, October 19, ?007 Page 1 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -208- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils Soil 2ype lvtollisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 . Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <.oD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <.oQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD LOD = limit ofdetection (0.114 kg/kg) LOQ =limit of quantitatia] (2 .0 llglkg) Fortified Recoveries Aerobic Anaerobic 10 .0 10,0 9 .8 10 .4 9.7 10.1 9 .8 8 .1 9 .9 9 .7 11 .7 10.9 12.1 11 .2 9.2 10 .2 7 .6 8 .0 ]oo% 100% 98% ]04% 97% 101% 98% 81% 99% 97x 117% 109% 121% 112% 92% 302% 76% saea 9.2 9 .9 8 .2 9 .5 9 .2 9.3 9.0 9,0 10.4 9 .2 11 .4 12 .1 11 .2 10 .9 92% 99% 82% 95% 91% 93% 90% 907G 104% 92% 114% 12]% 112% ]099G Friday, October 11, 2007 Page 2 oj4 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -209- Project No.; 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (pglkg) in Untreated and Fortified Soils Soil Type Altisol Sample Inlerval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <.OD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ FortYled Recoveries Aerobic Anaerobic 9.3 93% 9.3 93% 9.4 94% 11 .0 110% 10.1 lol% 8.6 96% 9,0 90% 10 .5 105% 11 .9 119% 11 .2 114% 9.3 93% 10.9 109% 10.7 107% 11 .2 l14% 10.9 109% 13 .3 133% 9.2 92% 9.3 93% 9.2 7.4 8 .7 9 .3 9 .0 9 .5 9 .7 9.6 11 .3 11 .4 9.0 9.6 11 .6 8 .6 94% 74% 87% 9376 90% 95% 97% 96% 113% 114% 90% 96% 116% 86% . LOD = limit of detection (0 .114 NgV LOQ = limit of quantitaiion (2 .0 ug/k8) Friday, October lY, 2007 Page 3 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -210- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils Soil Type Incentisol Sample Interval Day 0 Week 1 Week 2 Wcak 4 Month 2 Mouth 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <,OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ FortyledRecoveries Aerobic Anaerobic 13 .9 12.4 14 .6 11 .6 11 .3 11 .6 12 .1 11 .5 12.0 13.3 14.1 10.8 10 .7 9 .2 12 .8 10 . 9 9.5 9.2 139% 12454 w6w 116% 113% 116% 121% 115% 120% 133% 141% 108% lorn 92% 123% 309% 95% 92% 9 .9 9 .7 10 .7 9.6 10.8 10.5 10 .1 10 .7 11 .8 12 .2 10.9 11 .6 9.6 10.6 99% 97% ]07% 96% loa% los% lolos 107% 118% lux loa% 116% 96% 10696 LOD = limit of dotecfion (0 .11411g/kg) LOQ =limitofquantitation (2,0 pg/kg) Friday, Ocroba 17, 2007 page 4 aJ4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 211 - Project No. : 112E-108 WiYdrrfe International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFUA (jig/kg) . in Untreated and Fortified Soils Soil Type_ -__ Ultisol Sample interval Day Q Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <1,OQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Fortyled Recoveries Aerobic Anaerobic 8 .5 6.9 8 .2 8.2 7.6 7.8 8 .6 7.4 10.2 10 .1 9 .2 9 .3 4.1 8.4 7.2 6.2 ss% 69% 92% 82% 76s4 7B% 86'% 74% 301% loi% 92% 9345 41% 94% 72% 62% 8.5 ss% 8.8 8854 8.5 ss+a 8.4 94% 8.8 arlc 8.6 86% 9.1 91% 7.0 70% 9.8 98% 9.5 95% 9.4 94% 9.5 05% 8.8 889i 9.9 99% LOD - limit of detection (0.128 lxg/kg) LOQ =limit of qllantitation (2 .0 Ilg/kg) Friday, October !1, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 212 - Project No . : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (Kg/kg) in Untreated and Fortified Soils soil Type Mollisol ' Sample Interval DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <[,OD <LOD <],OQ <LOQ <LOQ <LOD <LOD <LOD Q.OD Q,OD <LOD <1.OD <LOD <LOD <LOD <LOD FortIfled Recoveries Aerobic Anaerobic 9.5 95% 9.6 96% 8 .7 87% 9 .3 93% 9 .0 90% 9.2 92% 8.7 87% 6.9 69% 8.4 94% 8 .4 84% 10 .1 lot% 10 .1 loi% 9.5 95% 8.7 97% 7.4 74% 8.8 BS% 6.1 61% 6.3 63% 8.2 ss% 8.6 86% 6.7 67% 7.6 76% 6.3 63% 7.1 7176 6.3 63% 8.0 a% 8.5 85% 7.5 75% 9.6 96% 9.4 94% 9.8 98% 7.9 79% LOD -limit of detection (0 .12811g/k8) LOQ - limit ofquantitation (2 .0 Ng/kg) 1:Slday, octobw 1?, 7007 p. 213 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 213 - Project No. : 112E-108 Wrldlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (jag/kg) in Untreated and Fortified Soils Soil Type Alfisol SamPle Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD 4.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <I.OQ <LOQ Fortified Recoveries Aerobic Anaerobic 9.2 92% 8 .7 87% 9.2 92% 10 .9 109% 9.4 94% 8 .0 80% 7 .7 77% 9.4 94% 10.3 103% 9.7 97% 7,5 75% 8 .9 89% 8 .9 89% 9 .3 93% 8.6 86% 12 .2 122% 8.7 97% 8.8 ss% 8.3 83% 6.1 61% 8.3 83% 8.6 06% 7.9 79% 7.9 79% 7.0 70% 7.6 76% 8.8 88% 9.9 99% 7.4 74% 7.2 72% 9.5 95% 6.7 67% LOD = limit of detection (0.128 pg/kg) LOQ =limit of quantitation (2 .0 pglkg) Friday, October 11, 2007 Company Sanitized . Does not contain TSCA C81 . Wildlife International, Ltd. -214- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (wg/kg) in Untreated and Fortified Soils Sample Soil T,ppe -- Interval Incentisot Day 0 Week 1 Week 2 Week 4 . Month 2 Month 4 Month 6 month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <1.OD <LOD <LOD <LOD <LOD <LOQ <,OQ <LOQ <LOQ <L ;oQ <LOQ <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD =limit ofdetection (0 .12811g/k8) LOQ =limit of quaniitation (2.0 pg/kg) Fordfied Recoveries Aerobic Anaerobic 12.0 11 .2 12 .5 10 .1 9 .2 10.3 9.8 8 .9 10 .4 11 .7 12 .2 8 .8 8,2 8 .3 9.9 .8.2 7 .4 6 .9 120% 112% 125% lol% 92% 103% 99% 99% 104% 117% 122% as% s2% 93% 99% e2% 74% 69% 7.9 79% 7.4 74% 7.9 79% 7.4 74% 7.4 74% 8.7 87% 8.0 ao% 8.5 as% 8.7 97% 10 .3 los% 6.9 69% 7.3 79% 7.0 70% 8.5 SS'.i Filday, October J.2, aoo7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 215 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (14g/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic Fortyled Recoveries ' Aerobk Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 289 283 244 247 210 208 165 147 102 ( 981 ( 45 1 ( 531 ( 41 ) <LOD <LOD <LOD <LOD <LOD us% 113% 97% 99% 24% 93% s6% 59% 41% 39% 18% 21% 16% o% o% o% o% o% 291 116% 288 115% 220 88% 258 103% 249 99% 181 7"1% , 179 71% 155 6290 165 66% 117 47% ( 87 ) 35% ( 54 ) 22% ( 681 37% LOD = lintit of dctection (22.3 Ilg/kg) LOQ= limit of quantitation (100 Itglkg) Values in parentheses an <LOQ and >=LOD Monday, October IS, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -216- Project No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (gglkg) in Untreated and Fortified Soils Soil TypeMollisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic Forti,fted Recoverles Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 299 110% 261 104% 213 85w 217 97% 194 73-A 112 45% 119 49x ( 51 ) 21% ( 63 ) 25% <LOD 0% ( 32 ) 13% <LOD e% <LOD 0% <LOD <LOD <LOD <.OD e% 266 106% 279 112x 244 flsx 273 109w 257 . 103% 243 97% 191 m% 192 77u ( 99) 4% 162 esx ( 70 ) 29% ( 46 ) 19% ( 36 ) 14% <LOD LOD = limit ofdetection (22.3 pWIW LOQ = limit ofquantitation (1001tglkg) Values in parentheses are <LOQ and >mLOD Monday, October 1S, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 217 - Project No, : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,1 10 Measured Concentrations of 8-2 FT4H (gg/kg) in Untreated and Fortified Soils Soil Type Alfisol Sample ---- I- nterval- Untreated Controls Aerobic Anaerobic Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 month is Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 307 123% 245 98% 210 84% 143 57% 156 62% 142 57% 117 47% 112 45% ( 75 ) 30% ( 791 32% ( 54 1 22% ( 51) 21% <LOD 0% ( 291 12% <LOD o% <LOD o% <LOD o% <LOD o% 267 107% 254 L02% 215 96% 203 sl% 205 82% 161 64% 127 51% 143 s7% ( 81 ) ss% ( 62 1 25% ( 50 ) 20% ( 37 ) 15% <LOD o% <LOD o% LOD - limit of detection (22 .3 llg/k8) LOQ = limit ofquantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD Monday, October 15,1007 Company Sanitized . Does not contain TSCA CBI, Wildlife International, Ltd. - 218 - Project No.: 112E-108 Wirdlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (ug/kg) in Untreated and Fortified Soils Soil Type Incentisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD ForfifiedRecoveries Aerobic Anaerobic 285 262 267 285 245 234 218 284 201 200 157 186 ( 86 1 ( 85 ) ( 55 ) 115 <LOD <LOD 114% 105% lors 114% 98% 94% a'1% 114% sl% 80% 63% 74% 34% 34% 22% 46% o% o% 287 115% 285 114% 258 los% 260 104% 237 95% 235 94% 230 92% 219 87% 134 54% 165 66% 106 42% 106 42% ( 42 ) 17% ( 371 13% LOD =limit ofdetection (22.3 !tg/kg) LOQ =limit of quantitation (100 llglkg) Values in parentheses are Q,OQ and >=LOD A-tonday, October 75, 3007 Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. - 219 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,710 Measured Concentrations of 8-2 k'TOH (gg/L) in Headspace Gases from Untreated and Fortified Soils Sail T,ppe Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Weak l Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 1S Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (12 .5 pg/L of headspace) Fordj7ed Soils Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 ' Pags I aj4 Company Sanitized . Does not contain TSCA CBI . Wildlzfe International, Ltd. -220- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (pg/f.) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic M0111s01 bay 0 Week 1 Week 2 Weck4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ m limit of quantitation (12.5 ug/L ofheadspaae) Fortified Soils Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ a.oQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 2 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 221 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (p,g/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Wak4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (12 .5 g/) of headapace) FortYled Soils AerobJC Anaerobio <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q .OQ <LOQ <LOQ <LOQ <LOQ <LOQ 4.OQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Page 3 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 222 - Projcct No.: 112E-108 ~ Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (Wg/Y,) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Incentisoi Day 0 Week 1 Wcok2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ -I,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit ofquantitation (12 .5 pglL of headspaco) Fortified Solls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ . <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ aronday, October IS, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -223Appendix II Measured Concentration of Selected Analytes (pmol/kg Soil Dry Weight) Company Sanitized . Does not contain TSCA CBI . Wildlfe International, Ltd. - 224 - Project No.: 112E-108 Wildlife Xnternatlonal, Ltd. Project Number 112E-1os Measured Concentrations of PFOA (Itmollkg) in Soils Treated with C Soil 7'ype Sample Interval Aerobic Live-------S-terile Day 0 <LOQ <LOQ Week 1 <LOQ 0.043 <LOQ <LOQ week 2 weex 4 0.046 0.077 0.088 0.140 0,148 <LOQ <LOQ <LOQ 0.005 <LOQ month 2 0.295 0.006 Month 4 0.362 0 .651 <LOQ 0 .005 Month 6 0 .903 0 .909 <LOQ 0 .012 1.065 <LOQ ' Month 9 1.176 0.019 Month 12 1 .349 0.888 <LOQ <LOQ Month 15 0.831 0.821 <LOQ Month 18 0 .876 0 .992 Month 24 0.794 0 .884 0.795 LOD - limit of detection (0.0005 )pmol/!cg) LoQ = limit of quantitatlon (0 .0048 pmovlcg) Anaerobic Live sterile <LOQ 4. .OQ 0.053 0.055 0.065 0.058 0.124 0 .097 0 .169 0.177 0.277 0.322 0.359 0 .253 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0 .006 <LOQ 0.007 <LOQ Thursday, November OB, T007 . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 225 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumber 112E-1os Measured Concentrations of PFOA (WmoUkg) in Soils Treated withc Soil _Type _ Sample Interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .037 0.036 0.043 0.044 0 .052 0 .049 0 .077 0.073 0.111 0.142 0.180 0 .180 0 .223 0.167 0.251 0.227 0.238 0,599 0 .307 0 .287 0.554 0.501 LOD = limit of detection (0.0005 pmol/kg) T.OQ= limit ofquantitation (0 .0048 lunoUkB) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.007 <LOQ 0.008 0.006 0 .008 0 .008 0 .007 0.008 Anaerobic Live Sterile <LOQ <L.OQ 0 .035 0 .036 0 .045 0.044 0.049 0.054 0.103 0 .104 0 .189 0 .149 0.284 0.220 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.006 <LOQ 0 .009 0 .006 Thwsdcy, November 08, 3007 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. - 226 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 1121?-108 Measured Concentrations of PFOA (umol/kg) in Soils Treated with C Soil 7ype Sample Interval Aerobic Live Slerile AlOsol Day 0 Waek 1 Wook Z Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q,OQ 0 .020 0 .020 0 .027 0.026 0 .036 0 .038 0.056 0.060 0.077 0 .080 0 .141 0.127 0.236 0.226 0 .158 0 .203 0.321 0.250 0.469 0.376 0.475 0.460 LOb =limit of detoedon (0.0005 jumoUkg) LOQ =limit ofquantitation (0 .0048 Eanol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0 Q <LOQ <LOQ <LOQ <LOQ <LOQ 0.008 <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 0.023 0.026 0 .034 0 .037 0.048 0.048 0.043 0 .075 0 .217 0.188 0.240 0.202 Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0 .005 <LOQ 0.005 <LOQ . Ffidqy, Oelober 12, 207 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 227 - Project No .: 112E-108 Wildlife International, Ltd. Project Number 1121?-108 Measured Concentrations of PFOA (gmol/kg) in Soils Treated with C Soil Z)rpe Sample Interval Aerobic Live dYerlle Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .046 0 .050 0.069 0.070 0.135 0 .132 0 .295 0 .236 0.460 0.457 0.639 0 .605 0 .697 0.820 0.888 0.845 0 .972 0 .930 1 .020 1 .078 1 .282 1 .338 LOD= limit of detection (0.0005 NmoUkg) LOQ =limit ofquantitation (0.0048 lunol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0,005 <LOQ 0,007 QAQ 0.011 <LOQ 0 .013 0 .007 0.015 0.007 0.010 0 .006 Anaerobic Live Sterile <LOQ <LOQ 0 .050 0.043 0.064 0.065 0.182 0.116 0.333 0 .349 0.687 0.731 0.790 0.838 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0,012 <LOQ 0 .018 <LOQ I Friday, October 1Y,1007 Company Sanitized . Does not contain TSCA CBI . Wirdlfe International, Ltd. Project No, : 112E-108 -2281: Wildlife International, Ltd. Project Number 112E-]08 Measured Concentrations of 8-2 FTUA (ulmollkg) in Soils Treated with( Soil Z'ype Sampk Interval Aerobic Live Sterile Ultisol Day 0 Week i Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOQ <LOQ <LOQ 0 .005 0.006 0.009 0.007 0.014 0 .015 0 .027 0.028 0.026 0.042 0.067 0.043 0 .013 0 .017 0,025 0.029 0.034 0.014 0 .034 0.071 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD QOD <LOD <LOD <LOD <LOD LOD= limit ofdetection (0.0003 itmoUkg) LOQ a limit ofquantitation (0 .0044 pmoUkg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOQ <1.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD F}fdny, October 11, 2067 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -229- Project No.: 112E-108 Wildlife International, Ltd. Project Number 11 2E- 108 Measured Concentrations of 8-2 FTUA (pmol/kg) in Soils Treated with' Soil Type Sample Intervol Aerobic Live Sterile Mollisol Day 0 Week 1 Wock2 Woek4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 19 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ Q.OQ QAQ <LOQ <LOD <LOD <LOQ <1.OQ 0 .007 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OQ <LOD <LOD LOD =limit ofdetection (0.0003 pmoUkg) LOQ = limit of quantitatiaa (O .OO44 Ixmol/kg) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD Frfday, Oaober 1$ 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 230 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-i08 Measured Concentrations of 8-2 FTUA (wmol/kg) in Soils Treated witht Santple S-oi-l ' e --------------Int_erval Aerobic Live Sterlk Anaerobic Live Sterile Alfisol Day 0 Week 1 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Week 2 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD WC&4 Month 2 Month 4 <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ 0 .008 <LOD <LOD <LOD <LOD <LOD Month 6 Month 9 <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD 0.005 0.005 <LOQ <LOD <LOQ <LOQ Month 12 <LOQ <LOQ <LOD <LOQ Month LS Month 18 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Month 24 <LOQ : <LOQ LOD =limit of detection (0 .0003 pmollkg) LOQ =limit of quantitation (0.0044 InnoUkg) FriAy, October ta, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 231 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (umol/kg) in Soils Treated wit4 Sotl Type__ Sample Interval Aerobic Live Sterile Incentisol Day 0 Week I Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD Q.OD <LOQ <LOQ 0 .013 0 .011 0 .055 0 .027 0.170 0.194 0.358 0 .350 0 .419 0 .352 0.457 0.452 0.492 0 .550 0 .559 0 .530 0.542 0.522 LOD = limit ofdetection (0.0003 Nmol/kg) LOQ -limit ofquantitation (0.0044 umol/Jcg) <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 0.006 Q.OQ 0,005 <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOQ 0 .017 <LOQ 0 .012 0 .012 0.028 0.028 0.034 0.022 <LOD <LOD <LOD <LOD Q.OD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ Friday, Ouober !2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -232-- Wildl fe International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (ILmoUkg) in Soils Treated withL Soil 4pe Sample Interval Aerobic Live Sterile Ultisol Day 0 Week 1 Wcck2 Woek 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOQ <LOQ 0.008 0.008 0 .013 0 .010 0 .018 0 .018 0.020 0.019 0.021 0.040 0 .041 0 .025 0 .006 0.009 0.009 0.008 0.014 0 .006 0 .010 0.011 LOD =liunitof detection (0 .0011 lrmmollkg) LOQ alimitof quantitation (0.0042 pmot/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q.OD <LOD <LOD <LOD 0 .004 Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Filday, Ocrobcr 12, 2607 Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. -233- V, Project No. : 112E-108 Wildlife International, Ltd. Project Number 11 2E- 108 Measured Concentrations of 8-2 FTA (ttmol/kg) in Soils Treated withC Soil Type_ _ Sample Interval Aerobic Live Slerile Mollisol Day 0 Weak 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ 0.005 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD ~ <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.oD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of dctcction (0 .0011 pmollkg) LOQ = limit of quantitation (0.0042 lunoft Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Friday, October ls 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -234I Wildlife International, Ltd. Project Number 112E-1Os Measured Concentrations of S-2 FTA (FxmoUkg) in Soils Treated witthE Soil 2~pe- Sample Interval Aerobic Live Sterile Alfisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0,0011 pmoUkg) LOQ = limit ofquantitation (0.0042 LunoUkg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <I,.OD <LOD <LOD <LOD <LOD Q.OD <LOD <LOQ n,day, October 1s,1oo7 Company SaniUzed . Does not contain TSCA CBI . Wildlife International, Ltd. -235- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (Rmol/Icg) in Soils Treated with~ Soil Type Sample Interval Aerobic Live Sterile Anaerobic Live Sterile Incentlsol Day 0 <LOQ <LOD <LOD <LOD <LOQ <LOD Q.OD <LOD Week 1 <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD Wcek 2 <LOQ <1.OD <LOD <LOD <LOQ <LOD <LOD <LOD Week 4 0.011 <LOD 0.014 <LOD 0.010 <LOD <LOQ <LOD Month 2 0.038 <LOD 0.005 . <LOD 0.027 <LOD 0.005 <LOD Month 4 0.154 Q.OQ 0.013 <LOD 0.158 <LOD 0.012 <LOD Month 6 0.341 0 .006 0.018 <LOD 0.302 <LOQ 0.011 <LOQ Month 9 0.343 0 .006 0.265 Q,OQ Month 12 0.279 <LOQ 0.294 <LOQ I i Month 15 0 .404 I 0 .430 I ! Month 18 0 .447 0.474 Month 24 0.423 0.464 LOD = limitof detection (0 .0011 pmol/kg) LOQ =limitof quantitation (0 .0042 lunoUkg) Fttaay, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 236 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112B-108 Measured Concentrations of PFNA (gmollkg) in Soils Treated witbL SoilType _ _ Sam,ple Interval Aerobic Live Staile Ultisol Day 0 Week 1 Week2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .015 0.016 0.025 0.026 0.038 0 .036 0 .052 0.046 0.060 0 .065 0 .061 0 .066 0 .072 0.065 0.018 0.034 0.051 0 .057 0 .064 0 .053 0.067 0.064 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit ofdetection (0.0003 funol/kg) LOQ m limit of quantitation (0 .0043 gmoft Anaerobic Live Sterile <LOQ <LOQ 0 .014 0.015 0.020 0.020 0.034 0 .029 0 .033 0 .046 0.050 0.048 0.065 0.055 <LOQ <LOQ <LOQ <LOQ <LOQ a,0Q <LOQ <LOQ <LOQ <LOQ <LOQ Q,0Q <LOQ <LOQ Friday, October 13, 2007, Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 237 - Project No.: 112E-108 WildYife Xnternational, Ltd. Project Number 112E-1Os Measured Concentrations of PFNA (pmol/kg) in Soils Treated with[ Soil _Type_ Sample Interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 week 4 Month 2 Month 4 Month 6 month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0,013 0.013 0 .012 0 .018 0 .023 0 .026 0 .039 0.037 0.048 0.048 0 .046 0 .043 0 .037 0.024 0.042 0.040 0.031 0 .056 0 .045 0 .037 0.055 0.075 <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L,OQ Q.OQ LOD = limit of detection (0.0003 Kmoft LOQ = limit of quantitation (0.0043 fanoUkg) Anaerobic Live Sterile Q,OQ <LOQ 0 .009 0 .009 0 .013 0,010 0.009 0.013 0 .014 0 .018 0 .026 0.020 0.034 0.026 <LOD <LOD <LOQ <LOQ <LOQ <i.OD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <1.OQ <LOQ F}Iday, October 12, 2007 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -238- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-10s Measured Concentrations ofPFNA (uluo,l/lcg) in Soils Treated witbr Soil 7ype Sample Interval Aerobic Live Sterik AIHsol , Day 0 Week 1 Wcok2 Weak 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.007 0 .009 0 .010 0 .008 0 .014 0 .017 0 .022 0 .035 0.023 0.029 0.019 0.034 0 .038 0 .050 0 .029 0 .038 0 .050 0.033 0.034 0.038 0.054 0.047 LOD = timit of deteotion (0.0003 WraoUkg) LOQ =limit of quantitation (0 .0043 ltmollkg) <LOD <LOD <LOQ Q.OQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ 0.005 0.006 0.007 0.010 0 .010 0 .008 0 .014 0 .019 0 .020 0 .024 0 .017 <LOQ <LOQ Q.OQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <T.OQ Fridar, Odober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 239 - Project No. ; 112E-108 Wildlife International, Ltd. Project Number 1 12E-1 08 Measured Concentrations of PFNA (gmol/kg) in Soils Treated with L Soi1 Type Sample Interval Aerobic Ltve Sterik Anaerobic Live Sterik Tnceutisol Day 0 Week 1 Week 2 Weak 4 Month 2 Month 4 Month 6 , Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.044 0.054 0.062 0.072 0 .079 0,076 0.134 0.138 0.128 0 .133 0 .146 0.172 0.172 0.171 0 .154 0 .152 0.159 0.160 0 .171 0 .174 0 .208 0.224 LOD =limit of detection (0.0003 Amol/W LOQ = limit of quantitation (0 .0043 lnnollkg) <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.021 0.016 0.028 0 .029 0 .075 0.047 0.053 0.057 0 .062 0 .077 0.073 0.078 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ P'Iday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 240 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (pmol/kg) in Soils Treated with L Soil Type Sample Interval Aerobic Live Sterile Anaerobic Live Sterile Ultisol Day 0 week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.009 0.009 0.015 0.016 0.021 0 .023 0 .035 0 .036 0 .052 0 .055 0.059 0.069 0.074 0.076 0.018 0 .041 0 .065 0 .066 0 .093 0.071 0.099 0.086 LOD = limit ofdetection (0 .0002 kmoUkg) LOQ limit of quantitation (0 .0039 umoUkg) <LOQ <LOQ <LOQ Q.OQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0 .009 0 .010 0 .013 0.014 0.018 0.016 0.026 0.029 0.049 0.055 0 .043 0 .038 ' <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Thursday, November 08, 3007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 --- ---- - ------ -241- Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (Wmol/kg) in Soils Treated withc Soil Type Sample Interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .007 0 .007 0 .009 0 .010 0.012 0.012 0.019 0.020 0 .028 0 .032 0 .034 0.034 0.032 0.024 0.038 0.036 0.028 0 .054 0 .043 0 .042 0.088 0,094 <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ LOD = limit of detection (0.0002 wmoUlcg) LOQ =1anit of quanfitation (0 .0039 pmoft Anaerobic Live Sterile <LOQ <LOQ 0.007 0 .007 0 .010 0 .009 0 .013 0 .013 0.021 0.021 0.034 0 .028 0 .041 0 .034 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Tbursday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . 'Wildlife International, Ltd. - 242 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (wnioUkg) in Soils Treated with[ Soil Type Sample Interval Aerobic Live Sterik A1Gso1 Day 0 Week 1 . Wcck 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month la Month 24 <LOQ <LOQ <LOQ 0 .004 0 .006 0 .006 0 .009 0.009 0 .016 0 .018 0,016 0,019 0 .018 0 .024 0.033 0.038 0 .025 0 .031 0.051 0.030 0.045 0 .040 0 .073 0.071 <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <y0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ LOD =limit of detection (0.0002 pmoVkg) LOQ = limit of quantitation (0,0039 Nmob'kg) , Anaerobic-Live Sterile <LOQ <LOQ <LOQ 0.005 0.006 0 .007 0 .011 0 .011 0.009 0.017 0 .025 0,026 0.028 0.024 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, OcYOber 11, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 243 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of l'FDA (wmo2/icg) in Soils Treated withc Sail ?ype Sample Interval Aerobic Live Sterile Yncentisoi Day 0 week 1 Week 2 Week 4 Month 2 Month 4 Month 6 . Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .009 0.010 0 .016 0 .016 0 .030 0.030 0,067 0.058 0 .085 0.098 0.102 0.134 0 .130 0.111 0.121 0 .136 0 .122 0.128 0.158 0.151 0,169 0.174 LOD =lirrut of detection (0.0002 itmoft LOQ =litnit of quantitation (0.0039 Itmol/kg) <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0,004 <LOQ 0,005 <LOQ 0.006 0.006 0.006 0.005 0.004 0 .004 Anaerobic Live Sterile <LOQ <LOQ 0.010 0,009 0 .015 0 .015 0.041 0.022 0 .042 0 .045 0.065 0.076 0 .091 0 .080 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CB1 . Wildlife International, Ltd. - 244 - Project No .: 112E-108 Wildlife International, Ltd. Project Number 11213-108 Measured Concentrations of PFUA (limol/kg) in Soils Treated with( Soil Type Sample Interval Aerobic Live Sterile Ultisol . Day 0 Week i Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 0 .005 0.006 0.009 0.008 0.015 0.014 0 .023 0 .027 0.028 0.029 0 .033 0 .030 0.006 0.019 0.027 0.028 0 .034 0.029 0.040 0.040 LOD alimitofdetection (0.0002 pmoUkg) LOQ =limit of quantitation (0 .0036 IunoUkg) <LOQ <LOQ Q,OQ <LOQ a.oQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ 0 .005 0.005 0.007 0.007 0.012 0 .015 0 .027 0.023 0.032 0.031 <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, Ootober 11, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -245- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations ofPFUA (~mol/kg) in Soils Treated w. ith( ,l Soil Type Sanrple Interval Aerobic Live Slerik MolGsol Day 0 Week l Week2 Week 4 Month Z Month 4 Month 6 Month 9 Month 12 Month 15 - Month 18 Month 24 <LOQ <LOQ <LOQ Q.OQ <LOQ 0 .004 0 .005 0.005 0 .011 0.010 0.018 0.019 0 .017 0.017 0.015 0.008 0.016 0.015 0 .011 0 .020 0 .018 0.016 0.026 0 .038 LOD = limit ofdetection (0.0002 Nmollkg) LOQ =limit ofquantitation (0.0036 1tmoUkg) <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 0 .004 0 .006 0 .011 0.008 0.012 0.010 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ Q.OD Fridaj, Oslaber ]2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 246 - Project No. : 112E-108 WbldlifC 114t8J"fZ6dti0)i6dlr Ltd. ProjectNumber 112E-108 Measured Concentrations of PFUA (pmol/kg) in Soils Treated with L Sanrple i4erobtc Anaerobtc Soil Interval Live Stoile Live Sterile AIGsol Day 0 W=k 1 Week 2 Week 4 Month 2 . Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOQ <LOQ <LOQ <LOQ 0 .004 0 .004 0 .008 0 .011 0 .007 0 .011 0.006 0 .011 0 .013 0 .020 0.014 0.016 0.024 0 .010 0 .012 0.013 0.025 0.018 LOD =limit of dctection (0.0002 fumoUkg) LOQ =limit of quantitation (0.0036 pmol/kg) <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ 0.005 0.005 0.006 0 .008 0 .006 <LOD <LOD <LOD <LOD Q.OQ <LOQ <LOD <LOD <LOQ <LOD <LOD <LOD Q.OQ <LOQ Friday, October 11, 2007 Company Sanitized, Does not contain TSCA CBI . Wildlife International, Ltd. -247- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA (pmol/kg) in Soils Treated with[ Soll Type Sample Intervol Aerobic Live Sterile Ineentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Moath 12 <LOQ <LOQ <LOQ 0 .004 0 .007 0.008 0.013 0.011 0 .034 0 .035 0 .033 0 .033 0 .037 0.050 0.052 0.050 0 .046 Month 15 Month 18 Month 24 0 .052 0.046 0.045 0.062 0.058 0.072 0 .080 LOD 31imit of detecaon (0 .0002 pmoUkg) LOQ =limit of quantitation (0 .0036 1tmoUkg) <LOQ ~ <LOQ <LOQ <LOQ Q,OD <LOD <LOD <LOD Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ Q,OQ <LOQ Anaerobic Live Sterile <LOD <LOD <LOQ <LOQ 0.006 0.006 0.017 0.011 0.025 0.025 0 .030 0.034 0.039 0.037 <LOD <LOD <LOD -COD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 248 - Project No.: 112E-108 Wildlife International, Ltd. Project Number l 12E-108 Measured Concentrations of 8-2 FTOH (pmol/kg) in Soils Treated with( Soil Type _ Sample Interval Aerobic Live Sterile Ultisol Day 0 Week i Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD Q.OQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD LOD = limit of detection (0:048 pmoUkg) LOQ =limit of quantitetion (0 .216 EtmoUkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q .OD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Monday, Ocfobsr IS, 2007 Company Sanitized, Does not contain TSCA CBI . Wildlife International, Ltd. - 249 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (jtuno1/kg) in Soils Treated withL Boll Type Sanqi/e Interval Aerobic Live Sterile Mollisol Day 0 Weok 1 week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month is Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit ofdetection (0.048 prnollkg) LOQ alimit ofqqantitation (0,216 ltcnol/kg) Anaerobic Live sterile <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Monday, October IS, 2007 Company Sanitized . Does not contain TSCA C81 . Wildlife International, Ltd. - 250 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (Itmol/Icg) in Soils Treated withC Soil Type Sample -Interval---- _- Aerobic Live Sterile Alfisol Day 0 Week 1 Week 2 Wak4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 75 Month 18 Month 24 <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD LOD = limit of detection (0 .048 EunoUkg) LOQ = limit of quantitation (0,216 kmoUkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Monday, Odaber 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -251 - Project No. : 112E-108 Wildlrfe International, Ltd. Project Namber 112E-108 Measured Concentrations of 8-2 M'OH (wmol/kg) in Soils Treated withl Soil Type Sample Aerobic _ Interval - Live Sterile Incentisol Day 0 week I Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ 0 .374 0 .269 0 .319 0 .297 <LOQ 0.302 0.216 <LOQ 0,282 0 .356 0 .374 0 .261 <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0 .048 Nmollkg) LOQ m limit of quantitation (0,216 Nmollkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOl) <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD Monday. Odober 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -252..... . . .... Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 F'TOH (wmoUL) in Headspaces Gases from Chambers Treated witlL Soil Type Sample Interval Aerobic Live Sterile Ultisol Day 0 Week 1 Week2 , Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Mouth 15 Month 18 Month 24 <LOQ Q,OQ a,oQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ - tin-it of quantitation (0 .027 Nmol/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October ls, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 253 - Project No. ; 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations, of 8-2 FTOH (Amol/L) in Headspaces Gases from Chambers Treated withC Soil Type Sample Intervol Aerobic Live Sterile Mollisol Day 0 Week 1 W=k 2 Wcek 4 Month 2 Month 4 Month6 mouth 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit ofquantitation (0.027 umol/L ofheadspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. - 254 - Project No. ; 112E-108 Wildlife International, Ltd. Project Number 112E-10s Measured Concentrations of 8-2 FTOH (p.moUL) in Headspaces Gases from Chambers Treated withC Soil Type - --- Sample Interval Aerobic Live Sterile Alfisol Day 0 Wxdc 1 Weak 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (0.027 ltmol/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <T.OQ Q.OQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ . Monday, October 15, 2067 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 255 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (Mol/L) in Headspaces Gases from Chambers Treated withE Soil_a'ppe - Sample Interval Aerobic Live Sterile Ynceatisol ' Day 0 Wcekl Week2 Week 4 Month 2 Month4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ QOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ a.oQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = linut of quantitation (0.027 luno1lL of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -256- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH Tol/lig) in Soils Treated with C Soil 7~pe Sample Interval Aerobic Live Sterile. Tllt7sot Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ Q.OQ <LOQ 0.321 0.401 <LOQ <LOQ Q.OQ <LOQ 0 .279 <LOQ <LOQ <LOQ <LOQ LOQ - limit of quantitation (0 .241tmollkg) Anaerobic Live SVerite Tleunday, November 08, ?007 Company Sanitized. Does not contain TSCA CBI . Wildlzfe International, Ltd. - 257 - Project No.: 112E-108 Wildlife International, Ltd. ProjectNumber 112E-108 Measured Concentrations of 7-2 s FTOH (umol/kg) in Soils Treated with Soil Type Sample Interval Aerobic Live Sterile Mollisol Day 0 Month 2 Month 6 Month 9 Month 12 Month L5 Month 18 Month 24 <LOQ <LOQ <LOQ <LpQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,QQ Q.OQ LOQ = limit ofquantitation (0 .24 pmollkg) Anaerobic Live Sterile 77suraday, November 00, 207 p. 258 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 258 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (Inol/kg) in Soils Treated with J Soil 7)vpe - Sample Interval _ Aerobic Live Sterile Alfsol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ Q,OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q..OQ LOQ =limit of quantitation (0.24 pmot/kg) Anaerobic Live Sterile Thursday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 259 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112B-108 Measured Concentrations of 7-2 s FTOH (praol/kg) in Soils Treated withL sample Soil Type - - - Interval Aerobic Live sterile Incentisol . Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Mouth 24 <LOQ <LOQ <LOQ <LOQ 0 .672 0 .672 0 .466 1 .121 0.466 0.287 0.418 0.440 <LOQ Q.OQ <LOQ Q,OQ LOQ = I'nnit of quan6tation (0.24 Nmol/kg) Anaerobic Live Sterile Thursday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlzfe International, Ltd. - 260 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (il.moUkg) in Soils Treated with[ Soil Type Sample Interval - --- Aerobic Live Sterile Uitisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q 0 .015 0.021 0.037 0.048 0.071 0.065 0.094 0 .076 0 .077 0 .091 0 .063 0.038 0.123 0.127 LOQ = limit of quantitation (0 .0045 Nrnol/kg) Anaeroblc Live Sterile Wednesday, Mareli 11,1008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -261- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (pmoUkg) in Soils Treated with[ Soll Type Sample I'nteryal Aerobic Ltva Sterik Molllsol . Day 0 Month 2 Month 6 Month 9 Mouth 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 0.005 0.006 0.006 0.006 0.011 0.011 0.015 0.020 0.008 0.008 0.020 0.015 LOQ - l'vnit of quantitation (0 .0045 pmol/kg) Anaerobic Live Sterile Wednas4ry, htmch 11,1oos Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 262 - p. 262 Project No.: 112E-108 Wildlfe International, Ltd. Project Number 112B-108 Measured Concentrations of 7-3 Acid (p.moUkg) in Soils Treated withc Soil Type Sample Interval Aerobic Llve Sterile Alf(sol Day 0 Month 2 month 6 Mouth 9 Mouth 12 Month 15 Month 18 Month 24 R,OQ <LOQ Q,OQ <LOQ 0.007 0.006 0.007 0.006 0.011 0.014 0,017 0 .020 0.018 0.015 0.015 0.016 LOQ - limit of quantitation (0.00451tmoUkg) Anaerobic Live sterile Wednesday, March 12,100d Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 263 - Project No. : 112E-108 Wildlife International, Ltd. ProjeotNumbor 1128-108 Measured Concentrations of 7-3 Acid ()lmol/kg) in Soils Treated witht Sofl Type sample Interval Aerobic Live Sterlle Inceptisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.038 0.022 0 .153 0 .185 0.290 0.347 0.626 0.557 0.590 0.655 0.638 0.480 0.803 0.728 LOQ=limit of quantitation (0 .0045 pmoykg) Anaerobic Live Sterile Wednesday, Mardi 12,100$ Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -264- Project No.: 112E-108 Wildlfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (p.moUkg) in Untreated and Fortified Soils Sail l)+pe_ Sample Interval Untreated Controls Aerobic Anaerobic Ultisol . , Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 month 15 Mouth 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ Q.OQ <1.OQ <LOQ LOD =limit of detection (0.000511mol/kg) LOQ = limit of quantitation (0 .0048 umol/kg) FordJled Recoverles Aerobic Anaerobic 0 .023 0.019 0.021 0.022 0.022 0.022 0 .025 0 .025 0.029 0.028 0,026 0.025 0.022 0 .024 0.018 0.017 95% 78% 86% 91% 929" 92% 104% 103% nzor.c 114% 106% 04% 92% 100% 74% 6816 0.023 0 .022 0,025 0.027 0.022 0.024 0,023 0.022 0 .027 0.026 0,027 0.027 0,027 0.028 95% 92% 105% 113% 92% 98% 93% 90% 114% 108% 114% 113% 112% 115% Friday, October R, 2007 page l of4 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. - 265 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measurea Concentrations of PFOA (pmol/kg) in Untreated and Fortified Soils Soil Type Sarple Interval Untreated Controls Aerobic Anaerobic Motlisol , Day 0 Week 1 Week 2 Woek 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limitofdetection (0 .0005 ppmol/kg) LOQ =limitof quantitation (0 .004811mol/kg) FortIfted Recoverles Aerobic Anaerobic 0 .022 0 .022 0 .023 0 .024 0.021 0.023 0,022 0.022 0 .026 0 .027 0 .032 0 .029 0.032 0.029 0.028 0.028 0.022 0 .025 s9% 93% 97% ]00% sx% 94% 91% 90% IO% 112% us% ]2l% Ill% 12094 114% 116% 97% 104% 0 .023 0 .024 0.020 0.023 0.020 0.021 0.023 0 .024 0 .03Q 0 .026 0.034 0.034 0.035 0.035 93% 101% as% 9691 es% 89% 95% 99% 122% 109% 140% 139% 14!% 143% Friday, October 12, 2007 Pap 2 of! Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - Project No.: 112E-108 266-Wildlife International, Ltd. Project Numbers 1128-108,109,110 Measured Concentrations of PFOA (itmoUkg) in Untreated and Fortified Soils Soil Type Sampk Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 <LOD <LOD <LOD <LOD Week 1 <LOD Q.OD <LOD <LOD Week 2 ' <LOD <LOD <LOD <LOD Week 4 <LOD <LOD <LOD <LOD Month 2 <LOD <LOD . <LOD <LOD Month 4 <LOQ <LOD <LOQ <LOD Month 6 <LOD <LOD <LOD <LOD Month 9 <LOD <LOD Month 12 <LOD <LOD Month 15 <LOD Month 18 <LOD Month 24 <LOD LOD =limit of detection (0.0005 Funol/kg) LOQ =limit of quantitation (0.0048 umoVkg) FortifFedRecoveries Aerobic Anaerobic 0 .022 0 .020 0.022 0.024 0.024 0 .023 0 .024 0 .024 0.026 0.026 0.026 0.028 0.027 0.027 0.028 0 .030 0 .022 0 .022 92% 83% 99% 100% 99% 94% 100% 101% 1 10% 107% los% JIM 1 10% lu% 115% iu% 92% 93% 0 .022 0 .020 0.021 0.022 0.023 0.024 0.025 0.025 0.025 0 .026 0 .027 0.026 0.025 0.027 91% 82% aT% 93% 96% 10D% 106% 104% 104% 107% llu% 109% 105% 112% I}iday, October 17, 2001 Page 3 0/'4 Company Sanitized . Does not contain TSCA C81 . Wildlife International, Ltd. - 267 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNurnbers 112E-108,109,110 Measured Concentrations of PFOA (itmol/kg) in Untreated and Fortified Soils Soil Type Sampie Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <I .OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit of detection (0.0005 pmoVkg) LOQ =limit of quantitation (0 .0048 lunoUkg) FortiJled Recoveries Aerobic Anaerobic 0.025 0.025 0.031 0 .024 0.026 0 .026 0.030 0 .027 0 .027 0.028 0.032 0.026 0.029 0.026 0.035 0.030 0.023 0.023 104% 103% 129% 10176 106% 109% 124% U2% 113% 117% 134% 108% 121% 106% 145% 124% 96% 9695 0.023 97% 0.023 94% 0.026 109% 0.024 98% 0.026 109% 0.025 105% 0.026 107% 0.026 109% 0.029 119% 0.027 111% 0.026 106% 0.028 115% 0.027 113% 0.028 116% Frfday, October 12, 2 007 Pnga 4 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 268 - Project No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 MA (pmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.0003 pmoVkg) LOQ =1vnit of Quantftation (0 .0044 Nmolnk$) Fordfied Recoveries Aerobic Anaerobic 0 .016 72% 0.014 65% 0.017 774i 0.017 79% 0.015 69% 0.015 69% 0.016 72% 0.016 72% 0.019 99% 0.017 so% 0.014 65% 0.016 73% 0.006 29% 0.012 S4% 0.011 5196 0.009 40% 0.017 so% 0.016 ~r4% 0.017 77% 0.019 87% 0.018 94% 0.018 82% 0.018 82% 0.017 79% 0.020 93% 0.017 76% 0.018 92% 0.017 ao% 0.014 65M 0.015 70% Friday, Ocfober 12, 2007 Page 1 of 4 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -269- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 WaJc 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month Is Month 18 Month 24 4.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.0003 pmoUkg) LOQ =limit ofquantitation (0.0044 gmoUkg) <LOD Q,OD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD d..OD <LOD Fortified-Recoverks Aerobic Anaerobic 0.020 92% 0.020 90% 0.018 81% 0.019 ss% 0.020 91% 0.021 93% 0.020 90% 0.020 91% 0 .019 ss% 0 .019 e7% 0 .022 200% 0.020 94% 0.022 loru 0.018 82% 0.017 79% 0.020 90% 0.013 59% 0 .014 63% 0.016 n% 0.019 8e% 0 .017 ~0 .019 87% 0 .017 0.021 98% 0.018 sox 0.018 8086 0.021 97% 0.017 78% 0.022 99% 0.023 107% 0.022 loax 0.019 esw Friday, Odober 1? 2007 Page 1 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -270- Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (pmoU[ig) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q .OD <LOD <LOD <LOD <LOD <LOD LOD = liniit of detection (0 .0003 lanoUkg) LoQ - limit ofquantaanon (0.0044111novkg) <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD ForllJled Recoverks Aerobic itnaeroblc 0.018 0 .017 0 .019 0 .021 0 .023 0.017 0.016 0 .020 0 .021 0 .020 0.014 0.020 0.018 0.020 0.018 0 .024 0 .014 0.015 84% 77% 86% 98% 105% 76% 75% 92% 96% 91% 66% 90% 14% 909i 80% 111% 62% 67% 0.020 0.016 0.020 0.021 0 .020 0 .019 0 .019 0.020 0.024 0.024 0.018 0.018 0 .023 0 .018 99% 75% 93% 95% 92% 89% 86% 94% 109% 108% 81% 93% 103% 83% F}lday, October 12,1007 Page 3 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 271 - Project No. : 112E-108 Wrldlfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (Itmollkg) in Untreated and Fortified Soils Sotl Y)pe- Sample Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit ofdetection (0 .0003 pmoUkg) LOQ =limit of quantitation (0.0044 umol/kg) Fbrfifed Recoveries Aerobic Anaerobic 0 .024 0.021 0.024 0.018 0 .019 0.021 0.020 0.017 0,018 0 .020 0,022 0.018 0.015 0.015 0.016 0 .014 0.012 0.011 11o+e 96% los% 83% a:% 97% 94% 79% BO% 89% 101% sl% 70% 68% 74% 64% ss% 52% 0.017 79% 0.018 8l% 0.018 8344 0.016 75% 0.019 95% 0.017 78% 0.017 77% 0.018 82% 0.017 76% 0.019 88% 0.014 ss% 0.015 70% 0.013 61% 0.016 71% Friday, Odober11,1007 Page 4ojl Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -272- Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (wmol/lcg) in Untreated and Fortified Soils soy T,ype Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD LOD =limit of deteo6on (0.0011 11moUkg) LOQ = limit ofquantitation (0.0042 tlmoUkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD ForttJied Recoveries Aerobic Anaerobic 0.013 61% 0.012 5e% 0.013 62% 0.014 69% 0.011 54% 0.011 55% 0.011 54% 0.011 53% 0.007 33% 0.005 24% 0.007 34% 0.008 37% <LOQ <LOQ 0.009 41% <LOQ 0.014 67% 0.014 66% 0.015 70% 0.015 69% 0.013 61% 0.014 65% 0.011 53% 0.013 63% 0.015 72% 0.011 51% 0.010 49% 0.010 46% 0.007 35% 0.007 12% Friday, Ouober 1$ ?007 Page J o/'4 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. - 273 - Project No.: 112E-108 Wildlife International, Ltd. Project: Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (ilnoUkg) in Untreated and Fortified Soils Soil Type_ _-_ISnatmeprvlael Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 ' Week 2 week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD 0 .006 <LOD <LOD Q,OD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0.0011 krnollkg) LoQ =limit of qaantitation (0.0042 kniolAcg) ForrifiedBecoveries Aerobic Anaerobic 0.018 97% 0.019 90X 0.018 94% 0.019 90% 0.019 90% 0 .018 88% '0 .017 83% 0.017 81% 0.016 78% 0.017 79% 0.017 94% 0 .014 69% 0 .013 64% 0 .013 6296 0 .010 50% 0.013 62% 0.005 23% <LOQ 0.015 70% 0.018 87% 0.015 71% 0.016 76% 0.013 6a% 0.017 83% 0.013 63% 0.015 70% 0.017 saic 0.015 71% 0.012 sa% 0.014 65% 0.012 57% 0.011 52% Friday, October 12, 7007 Page 2 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -274- Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (pmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 . Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <.OD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ LOD m limit of detection (0.0011 kmoft LOQ -limit of quantitation (0.0042 uniol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ Forti,/ied Recoveries Aerobic Anaerobic 0.016 72% 0.017 79% 0.016 78% 0.018 84% 0.022 106% 0.018 s7% 0.014 68% 0.017 94% 0.016 73% 0.015 70% 0.009 42% 0.014 67% 0.013 60% 0.014 66% 0.011 51% 0.016 77% 0.008 ao% 0.008 37% 0.018 Bi% 0.014 cr% 0.018 ts% 0.018 aa% 0.018 94% 0.017 s1% 0.016 77% 0.017 93% 0.018 ss% 0.017 93% 0.014 67% 0.014 66% 0.015 7344 0.015 70% Y)-Jday, October 11,1007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. - 275 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112U108,109,110 Measured Concentrations of 8-2 FTA (umol/kg) in Untreated and Fortified Soils Soil 7ype Sarnple Interval Untreated Controls Aerobic Anaerobic IncenHsol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD Q,OD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD a.OD <LOD <LOD <LOD <LOD <LOD LOD - limit of detection (0 .0011 Nmotlkg) LOQ =limit of quantitation (0 .0042 umoUlcg) Fort{/led Recoveries Aerobic Anaerobic 0 .022 lo"A 0 .020 %% 0.020 92% 0.016 76% 0.015 70% 0.018 84% 0.015 71% 0.013 63% 0 .011 $s% 0 .012 58% 0 .011 53% 0.009 45% 0.009 43'M 0.008 37% 0.007 34% 0.007 34% 0.004 2191 <LOQ 0.016 7e% 0.015 nx 0.015 7'+ 0.014 67% 0.015 72'K 0.016 76's 0.012 59% 0.014 "% 0.010 49% 0.013 63% 0.008 36% 0.007 34% 0.005 26% 0 .007 33% I-1riday, Octoberla, aaoy 1"ago 4o/4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -276- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (Amollkg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Ultisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month4 Month 6 Month 9 Month 12 Month 13 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =litnit of detection(0 .0003111no1/kg) LOQ a limit of quantitation (0 .0043 plnol/kg) FortiJied Recoverks Aerobic Anaerobic 0,020 0.017 0 .021 0 .021 0 .021 0 .021 0 .022 0 .022 0 .024 0 .024 0.022 0.023 0.015 0.022 0 .017 0 .014 94% 79% 9796 98% 93% 99% 104% iol% 114% 114% 103% 306% 70% ]03% 79% 67% 0,022 0 .021 0 .021 0 .023 0 .021 0.022 0.022 0.021 0.024 0 .024 0 .025 0 .025 0 .023 0 .025 loo% 99% 99% 307% 9rx 103% 107% 96% 112% llo% 116% 117% 108% 116% Friday, October 12, 2007 Page I of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -277- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (1tmoUkg) in Untreated and Forbifxed Soils Soil Type- -- Saraple --I--n-t-e-r--v-a-l - --- Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 month is Month 18 Month 24 Untreated Controls -Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q.OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD 4,01) <LOD <LOD <LOD <LOD LOD = limit ofdctection (0.0003 plllollkg) LOQ = limit ofquantitation (0.0043 lunoUkg) ForBfied Recoveries Aerobic Anaerobic 0 .020 94% 0 .021 96% 0.021 97% 0.021 97% 0.021 93% 0.021 99% 0.022 102% 0.018 96% 0.023 lar% 0 .023 106% 0 .026 122% 0 .026 119% 0 .026 120% 0 .025 117% 0 .022 las% 0.024 112% 0.017 slx 0.018 93% 0 .021 0 .022 0 .019 0.021 0.021 0.021 0.020 0.021 0 .023 0 .022 0 .025 0 .028 0 .026 0 .026 96ss 301% 89% 98% 96% 99% 93% 98% 107% 101% 116% 128% 122% 1 l9% Friday) October 11, 2007 Page ? of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -278- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (wmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated ControLv Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 19 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <i.OD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD LOD = limit of detection (0.0003 lunoUkg) LOQ = litnit of quantitation (0 .00431unollkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ ForBfeed Recoyerles Aerobic Anaerobic 0.021 0.020 0 .020 0 .024 0 .022 0 .020 0 .022 0 .023 0 .024 0 .024 0.022 0.025 0 .023 0 .025 0 .024 0 .026 0 .019 0.018 9e% 93% 94% 112% 103% 91% 103% 107% 113% 110% 100% 114% loa% 114% 113% 123% 87% 86% 0.021 0.017 0.020 0.022 0.021 0 .022 0 .022 0 .022 0.024 0.024 0.023 0.023 0.025 0.022 97% 81% 94% 101% 98% 101% 103% 100% uo% 110% 107% 1079% lls% 101% FNday, Odober 12, 2007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -279- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (ju.mol/Ikg) in Untreated and Fortified Soils Soil Type- Sample Interval Untreated Controls Aerobic Anaerobic Inceotisot Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOQ <i,OQ <LOQ <LOD <LOQ <LOD <LOQ Q.,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <1.OQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ LOD = limit ofdetection (0 .0003 pmoUkg) LOQ = limit ofquantitation (0.004311moUkg) FortlJled Recoveries Aerobic Anaerobic 0 .027 124% 0,025 116% 0.028 130% 0.023 106% 0.023 107% 0.023 107% 0.027 125% 0 .023 107% 0 .024 112% 0 .026 119% 0 .030 140% 0.023 las% 0.022 104% 0.021 100% 0.028 130% 0.023 109% 0.019 ar.6 0.019 se% 0.022 102% 0.022 100% 0.023 108% 0.021 98% 0.022 103% 0.022 102% 0.022 101% 0.023 107% 0.024 111% 0.024 111% 0.024 110% 0 .023 108% 0 .022 101% 0 .023 los% Friday, Ocroberll, 2007 Page 4 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 280 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (p.moUkg) in Untreated and Fortified Soils Soil Type _- Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <i.OQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q.OQ <LOQ <LOQ LOD = limit of detection (0.00021unol/kg) LOQ =limit of quantitation (0 .003911moVkg) Fortlfied Recoveries Aerobic Anaerobic 0 .018 93% 0 .014 74% 0.017 89% 0.017 86% 0.017 BS% 0.017 ~89% 0.018 94% 0.018 93% 0.021 l09% 0 .021 to9% 0 .019 96% 0 .019 100% 0.010 52% 0.018 95% 0.016 84% 0 .014 70% 0 .018 0.018 0.019 0.019 0.019 0.018 0.019 0.016 0.020 0.020 0.021 0.021 0.019 ().020 92% 91% 90% 97% 94% 92% 93% so% 104% 10:% uo% 109% 96% 104% Frlday, OMOber 13, 2007 Page t of4 p. 281 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. - 281 - Project No.: 112E-108 Wildlife International, Ltd. - Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (p.moUkg) in Untreated and Fortified Soils Soil Type -- Sample Interval UntreafedConrrols Aerobic Anaerobic Mollisal Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month.6 mouth 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD 4.OD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limitof detection (0 .0002 pmol/kg) LOQ = limit of quantitation (0 .0039 limollkg) ForliffedRecoveries Aerobic - Anaerobic 0.019 100% 0.019 loq% 0,019 9&% 0.020 304% 0.019 97% 0 .020 101% 0 .019 98% 0 .016 at% 0 .019 99% 0 .019 97% 0 .023 117% 0.021 109% 0.024 121% 0.022 112% 0.018 92% 0.020 102% 0.015 76% 0.016 80% 0.018 92% 0.019 99% 0.016 82% 0.018 95% 0.018 92% 0.018 93% 0.017 90% 0.018 90% 0.020 104% 0.018 92% 0.022 t14% 0.024 121% 0,022 112% 0.021 109% ' Friday, October 1?, 2007 Pass 2 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 282 - Project No.; 112E-108 Wildlzfe International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (jcmoUkg) in Untreated and Fortified Soils ' soilJType Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Weok 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ LOD = limit of datection (0 .0002 Nmol/kg) LOQ =limit ofquantitation (0 .003911moUkg) ForlifledRecoveries Aerobic Anaerobic 0 .018 0 .018 0 .018 0 .021 0 .020 0.017 0.018 0.020 0.023 0 .022 0 .018 0 .021 0 .021 0 .022 0.021 0.026 0,018 0.018 93% 93% 9456 110% 101% 96% 90% 105% 119% us% 93% 109% ]07% 112% ]09% 133% 92% 93% 0.018 92% 0.014 74% 0.017 V% 0.018 93% 0.018 90% 0.019 93% 0.019 917% 0.019 96% 0.022 113% 0.022 114% 0.018 90% 0.019 96% 0.023 116% 0.017 96% I~}fday, October J2, 2007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBi . Wildlife International, Ltd. - 283 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (wmol/kg) in Untreated and Fortified Soils Soil Type Sampk Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q .OQ <LOQ <LOQ <LOQ LOD =limit ofdetection (0 .0002 NmoUkg) LOQ = limit ofqllanritation (0 .0039 kmoUkg) ForH, jted Recovertes Aooblc Anaerobic 0.027 139% 0.024 12456 0.028 146% 0.023 116% 0.022 t13l6 0.022 116% 0.024 121% 0.022 115% 0 .023 120% 0 .026 tsa% 0 .027 t41% 0.021 108% 0.021 107% 0.018 91Si 0.025 129% 0.021 Jo9x 0.018 95% 0.018 92% 0.019 99l6 0.019 97% 0.021 107% 0.019 96% 0.021 108% 0.020 105% 0.020 101% 0.021 10756 0.023 lls% 0.024 122% 0.021 108% 0.023 116% 0.019 96% 0.021 to6% Friday, 0dober 12, 2007 rap 4 ofe Company Sanitized . Does not contain TSCA Cel . Wildlife International, Ltd. -284- Project No. : 112E-108 Wildrfe International, Ltd. Project Numbers 112B-108,109,110 Measured Concentrations of PFUA (ItmoUkg) in Untreated and Fortified Soils Soil Type -- - Sample Interval Untreated Controls Aerobic Anaerobic Ultisol bay 0 Weck 1 Week 2 Weok 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LUQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <1.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD - limit ofdetection (0.0002 ItmolJkg) LOQ = limit of quantitation (0 .0036 FunoUkg) Forli,Jled Recoveries Aerobic Anaerobic 0 .015 95% 0 .012 69% 0 .015 92% 0.014 82% 0.013 76% 0.014 78% 0.015 96% 0.013 74% 0.018 0.018 102% 101% 0.016 92% 0.017 93% 0.007 41% 0 .015 e4% 0 .013 n% 0.011 62% 0.015 95% 0.016 88% 0.015 ss% 0.015 84% 0.016 ss% 0.015 86% 0.016 91% 0.012 70% 0.017 98% 0.017 93% 0.017 94% 0.017 93% 0.016 ss% 0.018 99% Friday, Odober 12, 2007 Page 1 of 4 Company Sanitized . Does not contaln TSCA CBI . Wildlife International, Ltd. -285- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentc'ations of PFUA (ILmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Woak 1 Woek 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 1 S Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD LOD=limit of detection (0.0002 lunol/kg) LOQ = limit of.quantitation (0.0036 llmol/kg) Forttfied Recoveries Aerobic Anaerobic 0.017 95% 0.017 96% 0.015 87% 0.017 93% 0.016 90% 0.016 92% 0.015 87% 0 .012 69% 0.015 94% 0.015 84% 0.018 101% 0.018 lam% 0.017 95% 0.015 07% 0.013 74% 0.016 $a% 0.011 61% 0.011 63% 0.015 92% 0.015 86% 0.012 67% 0.014 76% 0.011 63% 0.013 71% 0.011 63% 0.014 so% 0.015 e5% 0.013 73% 0.017 96% 0.017 9496 0.017 99% 0.014 79% Friday, Oc(ober 12, 2047 Page 2 0/! Company Sanitized . Does not contain TSCA CBI . Wildlrfe International, Ltd. -286- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA ()tmoUkg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic ' A1Gsol Day 0 Weok 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 4.OD <LOD Q.OD <LOD <LOQ <LOQ LOD = limit ofdetection (0.0002 pmoUkg} LOQ =limit ofquantitation (0.00361unol/kg) Fortified Recoveries Aerobic Anaerobic 0 .016 9]% 0 .016 87% 0 .016 92% 0 .019 109% 0.017 94% 0.014 80% 0.014 7714 0.017 94% 0.018 103% 0.017 97% 0.013 75% 0.016 89% 0.016 89% 0.016 97% 0.015 86% 0.022 122% 0.016 87% 0.016 ss% 0.015 ss% 0.011 61% 0.015 83% 0.015 86% 0.014 79% 0.014 79% 0.012 70% 0.013 76% 0,016 88% 0.017 99% 0.013 74% 0.013 72% 0.017 95% 0.012 67% Friday, Odober 1?, 2007 Page 3 ojI Company Sanitized . Does not contain TSCA CBt . Wildlife International, Ltd. -287- Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFUA (p.nlol/lig) in Untreated and Fortified Soils Soil Type - Sample Interval Untreafed Conirols Aerobic Anaerobic Tnceutisol ' Day 0 Wak 1 Week 2 Week 4 Month 2 Month 4 Month 6 month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD = fin-it ofdeteotion (0 .000211moUkg) LOQ =limit ofquantiiation (0 .003611mollkg) Fardfled Recoveries Aerobic Anaerobic 0.021 120% 0.020 117% 0.022 125% 0.018 101% 0.016 92% 0.018 103% 0.017 98% 0.016 S9% 0 .018 to4% 0 .021 117% 0 .022 ln% 0.016 ss% 0.014 82% 0.015 93% 0.018 99% 0.015 sz% 0.013 74% 0.012 69% 0.014 79% 0.013 74% 0.014 79% 0.013 74% 0.013 74% 0.015 87% 0.014 so% 0.015 $s% 0.015 97% 0.018 103% 0.012 69% 0.013 73% 0.012 70% 0.015 as% Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 288 - Project No.: 112E-108 Wildlife International, Ltd. projectNumbers 1i2E-i08,109,110 Measured Concentrations of 8-2 FTOH (wmol/Icg) in Untreated and Fortified Soils Sell Type_ Sample Interval_ _ Untreated Controls Aerobic Anaerobic ForfifledRecovedeB Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <1.OD <LOD <LO?J <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD ' <LOD Q.OD 0 .622 115% 0 .609 113% 0.525 97% 0.531 99St 0.452 94% 0.448 83% 0.356 6676 0.317 39% 0.220 41% <LOQ 39% <LOQ ts% <LOQ 21% <LOQ 16% <LOQ 9% <LOQ 9% <LOQ tl% <LQQ o% <LOQ o% 0.627 116% 0.621 115% 0.475 881t 0.555 lo3x 0.536 99% 0.390 n% 0.385 71% 0.334 62% 0.356 66% 0.253 47% <1.OQ 35% Q 12% <LOQ 27% LOD =limit ofdetection (0.048 pmol/kg) LOQ =limit ofquantitation (0 .216 pmol/kg) Monday, Ouober 15, 2007 Page l of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. ~- 289 - Project No. : 112E-108 Wildlife International, .Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (wn1oU[cg) in Untreated and Fortified Soils Son Type -- Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 <LOD Weak 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Mouth 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q .OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of dctectton (0.048 pmoUlcg) LOQ = limit of quantitation (0 .216 pmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD FordfiedRecoverles Aerobic Anaerobic 0.645 120% 0.562 14% 0.459 85x 0.469 V% 0.419 79% 0.241 51A 0.256 48% <LOQ 21% <LOQ ux <LOQ 7% <LOQ 13% <LOQ 3% <LOQ 4% Q..OQ <LOQ <LOQ `'6 <LOQ x 0 .574 0.602 i106<LOD 12% 0.526 99% 0.589 109% 0.554 103 0.524 0.412 7'm 0.413 77% <.OQ 4X 0.349 65% <LOQ 28% <LOQ laK <LOQ l+u <LOQ bx . Monday, October IS, 2007 Page 2 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlzfe International, Ltd. -290- Project No. ; 112E-108 Wildlife International, Ltd. ProjectNumbers 1I2E-108,109,110 Measured Concentrations of 8-2 FTOH (itmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol , Day 0 Week 1 Week 2 Week 4 Month 2 Mouth 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.048 pmol/&g) LOQ =limit of quantitation (0.216 utnoUkg) Fort{Jied Recoveries Aerobic Anaerobic 0.662 123% 0.527 98% 0.453 84% 0.309 57% 0.336 62% 0 .307 57% 0 .252 47% 0 .241 43% <LOQ 30% <LOQ 32% <LOQ 22% <LOQ 21% <LOQ 5% <LOQ 12% <LOQ 4% <LOQ rn <LOQ o% <LOQ o% 0.574 107% 0.548 102% 0.462 86% 0.437 si% 0.441 82% 0.347 64% 0.274 51% 0.308 57% <LOQ 33% Q.OQ 21% <LOQ 2094 <LOQ is% <LOQ 4% <LOQ Tw Monday, October 15, 2007 Page 3 o14 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. - 291 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 1121;-108,109,110 Measured Concentrations of 8-2 FTOH (ItmoUkg) in Untreated and Fortified Soils Soil Type SamPle .Tnter0al Untreated Controls Aerobic Anaerobic Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.048 iunoUkg) LOQ = limit of quantitation (0.21611n1ol/kg) Fortified Recoveries Aerobic Anaerobic 0 .613 0 .565 0,575 0.613 0.529 0.505 0.470 0 .612 0 .434 0 .430 0 .338 0.401 <LOQ <LOQ <LOQ 0 .248 <LOQ <LOQ 114% 105% loiw 114% 98% 94% s7x 114% al% llox ~63% 74% 34% 34% rl% 46% o% 0.619 115% 0.613 114% 0.555 103% 0.560 104% 0.511 95% 0.507 94% 0.495 92'% 0.471 97% 0.289 54% 0.356 66% 0.228 42% 0.228 42% <LOQ 17% <LOQ 15% Monday, October 15,1007 Page 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -292- p. 292 Project No.: 112E-1fl8 Wilddife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Wcek2 Wcck4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 13 month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quanNtation (0.027 ILmoUL ofbeadspace) Fort{Jied Soils Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, Oc7obcr 15,1007 Page 1 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -293.. ...... Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils San:ple Soe - Interval_ Untreated Controls Aerobic Anaerobic Motlisol . , Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ a.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (0.027 pmoUL ofheadspace) Fordfied Soils Amble Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, oerober is 2007 pav 2 o14 p. 294 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 294 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (umol/L) in Headspace Gases from Untreated and Fortified Soils Sample Untreated Controls Soil Interval- Aerobic Anaerobic Alftsol Day 0 Week 1 Week 2 Week 4 Month 2 Mouth 4 Month 6 Month 9 Month 12 MontL 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (0.027 lanoUL of headspace) ForHfted Soils Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, OatoberlS, 2007 Page 3 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 295 - Project No. : 112E-108 Wildlife lnternational, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (gmol/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample lnterval Untreated Controls Aerobic Anaerobic Incentisot Day 0 week 1 Week 2 VValc4 Month 2 Month 4 Month 6 ' Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ LOQ = limit of quantitat3on (0 .027 punoUL of headspace) ' FordJied Solls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ tLOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <7.,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Page 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -296- Appendix III Example Calculations A. Standard Curves by Linear Repression Standard curves were prepared by plotting the ratio of analyte concentration (pg/L = ng/mL) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid to the internal standard concentration (0 .75 ng/mL 2-13C PFOA for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA and 7-3 Acid and 100 ng/mL HDFD for 8-2 FTOH and 7-2 sFTOH) in solutions with known concentrations on the abscissa and the respective peak area responses on the ordinate . Weighted regression (1/x) was applied to analysis of the data to determine the equation with respect to the abscissa as shown below: A = [m x (C /Ci ) + b] x Ai where A A m C Ci b analyte area internal standard area slope analyte concentration (ng/mL) internal standard concentration (ng/mL) intercept Concentrations of analytes in the final solutions of processed samples, just prior to injection, were calculated using a rearrangement of the above equation: C = Ci x (A/A') - b m B. Residue Concentrations of 8-2 FTOH 8-2 FTA 8-2 MA, PFOA PFNA PFDA PFUA. 7-2 sFTOH and 7-3 Acid in Soil Extracts Residue concentrations (Ag/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation : Sample Concentration in itg/kg = (C) x NO x MO x (VQ) (W) x (Vit) x (Viz) where C Ve Vi, Vi2 Vr, Vn W Concentration (ng/mL) as determined above Extraction volume (125 mL) . First initial volume (mL) Second initial volume (if required) First final volume (mL) Second final volume (mL) Weight ofsample (25 g dry weight) . Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. - 297 - Project No.: 112E-108 Residue concentrations (gmol/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation : Sample Concentration in gmol/kg = C x molecular weight (gmol/gg) where C = Sample concentration (gg/kg) as determined above C. Procedural Recoveries in Fortified Controls Procedural recoveries were calculated for fortified control samples prepared in each test condition at known concentrations in 8-2 FTOH (250 pg/kg), 8-2 FTA (10.00 ltg/kg), 8-2 FTUA (10.00 pg/kg), PFOA (10.00 pg/kg), PFNA (10.00 ug/kg), PFDA (10.00 Etg/kg) and PFUA (10.00 gg/kg) . Percent recoveries of the each analyte were determined to provide confirmation that the method was performing adequately. Percent recoveries were determined by dividing the concentration of the analyte recovered in the fortified sample by the nominal concentration added as shown below: Percent Recovery - Ag/kg Found x 100% jig/kg Added p. 298 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Sampling Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 -298Appendix IV Duration of Incubation Actual Days of Incubation Alfisol 0 7 14 . 28 63 119 182 273 365 462 546 728 Inceptisol 0 7 14 28 63 119 182 273 365 462 546 728 Ultisol 0 7 14 28 56 119 182 273 364 462 553 728 Project No.: 112E-108 Mollisol 0 7 14 28 56 119 182 273 364 462 553 728