Document 4aEnzE6a19bXgX7BxG0OomDm1

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC116, FC-1 26, FC-169,or FC-143. (Octanoicacid,pentadecafluoroammonium saltC,AS # 3825-26-1) Remarks: The 3M productionlotnumber was 83. The testsample isFC143. It'psuritywas notsufficientclhyaracterizedt,hough currentinformation indicatesitisa mixtureof96.5 - 100% testsubstance and 0 - 3.5% C6, C7, and Cg perfluoroanalogue compounds. METHOD: Not noted. Type: Staticacute GLP: No Year completed: 1978 Species: Bluegilslunfish(Lepomismachrochitus) Supplier-Not noted. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Not noted Test fishage: Not noted. Length and weight: Average length 3.0 cm Average weight 1.2 g Pretreatment: Not noted. Test conditions: Dilutionwater: Carbon-filterewdellwater. Dilutionwater chemistry: Temperature: 19"C Dissolved Oxygen: 9.9 ppm pH: 7.6 .Stockand testsolutionpreparation:Not noted Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: Tanks containing16 litertsestsolution. Number of replicates:one Number of fishper replicate:20 Loading rate:1.5g/L Number of concentrations:fiveplusa blankcontrol Water chemistry during the study: Dissolved oxygen range (24- 96 hours): 5.6- 6.5 mg/L (controelxposure) 4.9 - 5.9 mg/L (750 mg/L exposure)* pH range (24- 96 hours): 7.8 (controelxposure) 7.6- 7.8 (750 mg/L exposurer 750 mg/L testgroup (thirhdighestconcentrationd)ata given because totalmortalitoyccurredinthe highesttestconcentrationswithin48 hours. Test temperature (24 - 96 hours):19-20*C -RESULTS Nominal concentrations:Bk control4,20, 560, 750, 1000,and 1350 mg/L Element value: 96-hourLC50 = 569 (500- 636) mg/L Element valuebased on nominal concentrations CONCLUSIONS The testsample 96-hour LCso forbluegilslunfishwas determined to be 569 mg/L witha 95% confidenceintervaolf 500 to 636 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking3. Testinglacksinformatioonn the method followed.No informationon stockortestsolutionpreparations.Sample puritywas not properlycharacterizeadnd itlacksanalyticaclonfirmationof testsubstance concentrations. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 3844. OTHER Last changed: 5/24/00 ENVIRCNMMTAL ENGINEERING LABORA"V AQUATIC TOXICIIY DATA SHEET T*ilt Ck12 Test Orgoni3U -M@Kt. Material ToSted Kvg. Wt. zxwu AYS. Size Test Solution Volum iters/Tank No. Organisms per dose Time Started '3'@f3V PN,-% Date Started fdafe Completed Dilution Water Carbon Filter - Well Water (Tamp, D.O.-g@9M, pH b Ihvostixator e?)Vw@ LC SO (95% Confidence Limits) L Cdn*c. No. rganisms 24 Hrs, D.O. 48 Hrs. D.O. 72 Hrs. xposed No.Dead 9H ova No.Dead PH pps No.Dead M D,O. 96 Hrs. D.O. pps iNo.Dead pH ro- can ti@o@ CA -ac -7-0 '7.-6T,l5-1 -Z ?It 6.0 Is -7.7 56 t> 5- -)-too 2- C> r. 20 -7?. !@.-O -7,6 C) 1 1-7-4).9 >r-l -2- . Tow. covamts: ot'Ic. 2 O"C