Document 4QDXRvjabZDVmaqo76K727a1

ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-783. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotwas not noted.The testsample isFC783 (cc784-1).Currentinformatioinndicatesitisa mixtureof1.0% PFOS, 2.0% Sultonefoamer,2.3% sodium octylsulfate1,.6% polyoxyethylene monooctylphenol ether,0.1% sodium laurylsulfate2,5.0% diethyleneglycol monobutyl ether,0.05% tolytriazolaen,d 67.95% water. The followingsummary applies toa mixture with incompletely characteilzectconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: Standard Methods, 1975,and USEPA Methods forAcute Toxicity Tests withFish,Macroinvertebratesand Amphibians, 1975. Type: Acute static GLP: No Year completed: 1979 Species: Lepomis macrochirus Supplier:Osage CatfisherieIsn,c.O,sage Beach, MO. Analyticalmonitoring: Dissolvedoxygen,pH, ammonia, and temperature. Exposure period: 96-hours Statisticamlethods: LC50 valuescalculateudsingthestabsticamlethods of Litchfieladnd Wilcoxon, 1949. Test fishage: Juveniles Length and weight: Average length 28.6 mm Average weight 0.60g (wet) Loading: 0.2g/L. Pretreatment:None. Test conditions: Dilutionwater: Wellwater Dilutionwater chemistry: Hardness: 255 mg/L as CaC03 Alkalinity: 368 mg/L as CaC03 pH: 8.2 Lighting: 16 hour daylightphotoperiod. Stock and test solution preparation: The testmaterialwas added directly tothe dilutiownater on a weighvvolume basis. Concentrations dosing rate: Once StabiliotfythetestchemicalsolutionsN:otnoted Exposurevessels:5 gallognlassvesselcsontaini1n5gL oftestsolution. Number ofreplicates2: Number offishperreplicate5: Number ofconcentrationfsi:veplusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 8.2- 8.3(contreoxlposure) 8.2- 8.3(1000 rng/Lexposure) Temperature range (0-96hours): 22-22 OC Totalammonia range (0-96hours): 0.68- 1.1mg/L (controelxposure) 0.60 - 1.1mg/L (1000 mg/L exposure) Dissolved oxygen range (0-96hours): 5.0- 8.7mg/L (controelxposure) 3.8- 8.7mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Bk control,100, 180,320, 560, and 1000 mg/L. Element values: 96-hour LC5o = >1000 mg/L Element valuesbased on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-783 96-hour LC50 forbluegilslunfishwas determined tobe >1000 nig/L,the highestconcentrationtested.A 20% mortalitwyas observed. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking2. Testingmeets the criterifaorqualitytesting. However, sample puritywas not properlycharacterizedand the studylacks analyticaclonfirmationoftheamount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by AnalyficaBlioChemistryLaboratoriesI,nc., Columbia,MO atthe requestofthe 3M Company, 3M Lab Request number 4896S,1979. OTHER Last changed: 6/26/00 ANALYTICAL 610 CHEMISTRY LABORATORIES. INC. P.O. Box 1097 o Columbia, MO 65205 o (314) 474-8579 Static Acute Bioassay Report #23897 Submitted To: 3M Company Attn: Dale L. Bacon P. 0. Box 33331 St. Paul, MN 55133 Acute Toxicity of 3M Sample No. 6 (F-r- 7k-?) to Bluegill Sunfish (Lepomis macrochirus) June 19, 1979 Submitted By: Analytical BioChemistry Laboratories, Inc. 7200 East ABC Lane P. 0. Box 1097 Columbia, Missouri 65205 Prepared By: Carl M. Thompson Aquatic Biologist Alan D. Forbis Aquatic Supervisor -7lt3 Date* Date Approved By: aomAiAAgliop.-(@a.@fs A.' Ault \QLA ity Assuarsanc Officer y A suran A IW "14 JJoshnosihnnc atory Mafnager -711,31-A Date Date SLIMMARY The acute toxicity of 3M Sample No. 6 to bluegill sunfish (Lepomis macrochirus) was assessed using the methods outlined by the Committee on Methods for Toxicity Tests with Aquatic Organisms (1). Water quality parameters of temperature, dissolved oxygen, pH and ammonia were measured throughout the test and were within acceptable limits. As a quality check, the bluegill sunfish were challenged with a reference compound, Antimycin A. The observed 96 hour LCSO and 95% confidence limits (C.I.) were within the 95% confidence limits reported in the literature (2), indicating that the fish were in good condition. The results of the four day static fish toxicity study using bluegill sunfish are sil arized below. Compound 3M Sample No. 6 Antimycin A 96-hour LCSO (95% C.I.) >1@000 mg/l 0.000029 mg/i (0-000021-0.000040 mg/1) A 96 hour exposure of test fish to a logarithmic series of concentrations from 100 to 1,000 mg/l resulted in only 20 percent mortality at the highest concentration. Also, the results indicated a 96 hour, no observed effect concentration of 560 mg/l. INTRODUCTION This static bioassay was performed at the aquatic bioassay laboratory of Analytical BioChemistry Laboratories, Inc., Columbia, Missouri, for 3M Company, from June 14 to June 18, 1979, as authorized in a letter from 3M Company on June 6, 1979 (Appendix I). The purpose of this test was to determine the 24, 48 and 96 hour LCSO levels for 3M Sample No. 6 to bluegill sunfish (Lepomis macrochirus). A preliminary range-finding study was conducted from June 12 to June 14, 1979, to determine the concentration range from the definitive bioassay. The study was performed following the procedures outlined in ABC Protocol Number 7601 (Appendix I). METHODS AND MATERIALS The procedures for static bioassay, as described in Standard Methods for Examination of Water and Wastewater (3) and Methods of Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians (1), were used in this experiment. The bluegill sunfish used in the test were obtained from Osage Catfisheries, Inc. in Osage Beach, Missouri. The fish were identified to species using the taxonomic keys developed by Eddy (4). All test fish were held in culture tanks on a 16 hour daylight photoperiod and observed for at -least fourteen days prior to testing. Fish culture techniques used were basically those described by Brauhn et. al. (5). A daily record of fish observations during the holding period, along with prophylactic and therapeutic disease treatments, is included in Appendix I. During this period, the fish received a standard commercial fish food (Rangen's) daily until 48 hours prior to testing at which time feeding was discontinued. The bluegill sunfish used for this experiment had a mean weight of 0.60 g and a mean standard length of 28.6 mm. Weight and length measurements were made on a representative group of fish prior to the test and are included in Appendix I. The static fish bioassay was conducted in five gallon glass vessels containing 15 liters of laboratory well water. Characteristicsof this dilution water are shown in Table 1. These vessels were kept in a water bath at 22*C (tl.O). The test fish were acclimated to the dilution water and test temperature and held without food 48 hours prior to testing. A 48 hour range-finding test was conducted to determine the concentration range for the definitive study. The preliminary test concentrations were set at 500 and 1,000 mg/l. Based on the results of this preliminary testing and results of toxicity tests with fathead minnows (Pimephale.@ promelas) by 3M Company, five concentrations of the test compound, ranging in a logarithmic series from 100 to 1,000 mg/l, with ten fish per concentration were selected for definitive bioassay. The test was conducted with duplicate five gallon glass vessels containing five fish each as authorized by 3M Company (Appendix I). The fish were added to the test chambers by stratified random assignment within 30 minutes after addition of toxicant aliquots. 2 The 3M Sample No. 6 standard was received on June 8, 1979, in good condition. The sample upon receipt was observed to be a yellow liquid and was stored at 220C. Sample purity was not provided; therefore, the test concentrations were prepared based on the total compound. The test concentrations were prepared by transferring appropriate weights directly to the test chamber. All standard weights and dilution values are listed in Appendix 1. RESULTS Table 2 presents the predicted LCSO values and 95% confidence intervals for 3M Sample No. 6 and the reference test against Antimycin A, a piscicide. These values were obtained by employing the statistical method described by Litchfield and Wilcoxon (6). Mortality rates, test concentrations, and water quality data are presented in Table 3. The dissolved oxygen concentration which stayed between 40 and 100% saturation was considered adequate for testing. The pH values remained consistent with the control throughout the test. The ammonia concentratioas were below the toxic limit (7). The study was conducted following the intent of the Good Laboratory Practice Regulations (8) and the final report was reviewed by Analytical BioChemistry Laboratories' Quality Assurance Unit. All original raw data was provided to 3M Company, with a copy retained at Analytical BioChemistry Laboratories. 3 TABLE 1 Chemical Characteristics of Well Water at ABC's Aquatic Bioassay Laboratory. Parameter Dissolved Oxygen pH Hardness (CaCO3) Alkalinity (CaCO3) Conductivity Total Ammonia (NH3) N03-N Ortho-Phosphate Aluminum Arsenic Cadmium Chromium Cobalt Copper Iron Lead Mercury Nickel Zinc DDVP Diazinon Disyston Methyl Parathion Malathion Ethyl Parathion Concentration 9.3 ppm 8.2 255 ppm 368 ppm 50 umhos/cm <0.05 ppm 0.15 ppm 0.10 ppm <0.01 ppm <0.001 ppm <0.001 ppm 0.001 ppm <0.001 ppm <0.01 ppm 0.012 ppm 0.009 ppm <0.0001 ppm 0.0157 ppm <0.01 ppm <40 ng/l <20 ng/l <20 ng/l <80 ng/l <110 ng/l <80 ng/l Amp MW CoT2ound .3M Sample No. 6 Antimycin A*** TABLE 2 The Acute Toxicity of 3M Sample No. 6 and Antimycin A to bluegill sunfish (Lepomis macrochirus) 24 hours >lpooo 0.000070 (0-000056-0.000088)** LCSO in milligrams/liter (ppm) 48 hours >1,000 0.000040 (0.000032-0.000050)** (0 *Bioassay as conducted at 22*C (tl.O), mean weight and length, 0.60 g and 28.6 mm. **95% confidence interval. ***Antimycin.A standard obtained from Sigma Chemical Company, Type III, crystalline, Lot 125C-( Ln TABLE 3 The mortality rates and water quality parameters during the acute toxicity test of 3M Sample No. 6 with bluegill sunfish (Lepomis macrochirus). mg/l Concentration Control 100 180 320 560 1,000 Percent Mortality Hours 24 48 96 0 0 0 0 0 0 0 0 0 0 0. 0 0 0 0 20 20 20 Temp. oc 22 22 22 24 hours D.O.* mg/l PH** 8.2 8.3 8.3 8.3 NH3*** mg/l 1.1 1.2 Water Quality fem-p. oc 22 22 48 hours D.O.* mit/l pH** 5.8 8.2 5.8 8.3 NH3**- mg/l 0.75 0.75 8.3 8.3 1.1 22 4.7 8.2 0.62 Temp oc 22 22 22 *Dissolved oxygen concentrations Dissolved Oxygen Probe (Extech Model 8012) used with an Extech Mode **pH - pH Probe (Fisher Model 13-639-108) used with an Extech Model 671 pH and mV meter. ***Total ammonia concentrations Ammonia Probe (Extech Model 8002-8) used with an Extech Model 671 pH LITERATURE CITED (1) Committee on Methods for Toxicity Tests with Aquatic Organisms (C. E. Stephan, Chairman). 1975. Methods for Acute Toxicity Tests with Fish, Macro invertebrates and Amphibians. Environmental Protection Agency, Ecological Research Series EPA-660/3-75-009, April, 1975. 61 p. (2) Berger, B. L., R. E. Lennon and J. W. Hogan. 1969. Laboratory Studies on Antimycin A as a fish toxicant. U. S. Department of Interior, Investigations in Fish Control No. 26. 21 p. (3) American Public Health Association. 1975. Standard Methods for the Examination of Water and Wastewater. 14th ed. Washington, DC. 1193 p. (4) Eddy, Samuel. 1969. The Freshwater Fishes. 2nd ed. W. C. Brown Company, Dubuque, IA. 286 p. (5) Brauhn, J. L. and R. A. Schoettger. 1975. Acquisition and Culture of Research Fish: Rainbow Trout, Fathead Minnows, Channel Catfish and Bluegills. Environmental Protection Agency, Ecological Research Series EPA-660/3-75-011, May, 1975. 45 p. (6) Litchfield, J. T., Jr. and F. Wilcoxon. 1949. A Simplified Method of Evaluating Dose-Effect Experiments. Jour. Pharm. Exp. Ther. 96:99113. (7) National Academy of Sciences. 1971. Water Quality Criteria, 1972. U. S. Department of Commerce, PB-236 199. 592 p. (8) Food and Drug Administration. Regulations for Good Laboratory Practice. Federal Register, Vol. 43, No. 247, 59986-60025, December 22, 1978. 7 Quality Assurance Statement for final report #23897 entitled$ "Acute Toxicity of 3M Sample No. 6 to bluegill sunfish (Lepomis macrochirus)," for Dale L. Bacon, 3M Company, St. Paul, Minnesota-. In accordance with ABC Laboratories intent that all studies conducted at our facilities are designed and function in conformance with good laboratory practice regulations and the protocols for individual laboratory studies, an inspection of the final report for 3M Sample No. 6 was conducted and found to be in an acceptable form by a member of our Quality Assurance Unit. An inspection of the daily mortality rate of the test organisms prior to the initiation of the study indicated they were in good health and should not bias the observed mortality in the study. It was also noted that since the purity of the compound being tested had not been supplied, the LCSO would have to be calculated based on nominal concentrations of the total compound. A final inspection of all data and records on June 22, 1979, indicated that the report submitted to you is an accurate reflection of the study as it was conducted by ABC Laboratories. Should you have any questions relating to the information provided in this statement or the function of our Quality Assurance Unit, please contact me at your convenience. QJ4m'A"iatAy AA"ult As Q ity Assusulrtaance Officer Date 8