Document 4Jve4de33a9q84X88MweMEJ0Q

A R &S.6 _ O W BIODEGRADATION (BOD/COD) T E S T SU BSTAN CE___________________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFO S, FC-95, or as a component of FC-3041. (1Octanesulfonic acid) (C A S # 2795-39-3). Rem arks: The 3M production lot number was 2300. The test sample is FC3041 (cc 809-1). Current information indicates it is a mixture of 6% PFO S, 36% diethylene glycol butyl ether, 36.05% water, 12% Sultone foamer, 9% sodium octyl sulfate, 0.55% sodium lauryl sulfate, and 0.4% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the biodegradability o f the fluorochemical component o f the test sample. M ETH O D :______________________________________________________________________ Method: BOD Protocol of EA R (2129180). Type: Aerobic G LP: No Year completed: 1981 Contact time: 20 days Inoculum: Stale sewage, Chemolite, Cottage Grove, MN. R ESU LTS___________________________________________________ Biochemical Oxygen Demand (BOD): 5-Day: 12-Day: 20-Day: 16 4,0 00- 329,000 mg/kg 645,000 mg/kg 642,000 mg/kg C O N C LU S IO N S No conclusions given. Submitter: 3M Company, Environmental Laboratory, P.O . Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________________________ Reliability: Klimisch ranking 3. The study lacks description and records of the method followed. The inoculum is not properly characterized. There is no characterization of the test substance purity, and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. The testing was conducted on a mixture of chemicals having varing degrees of biodegradability, leading to potential misinterpretation of the results. 002866 REFEREN C ES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6508S, 1981. O THER________________________________________________________________________ Last changed: 6/26/00 002867 838300 vV  ./ - : Eortrlronmantal Laboratory BOD Work Staat Sampiai LabReq. N o .| Anelytt S j , a. s Z M z r " ' . g Date Started . Z/13Jti - ,-f BpD R unN o.l % f - / Day BOD Readings p . 4. S i/ L J f - fC-ZQtf} t L oT 1*000Sample OMcription ( CC S 0 9 - i ) Date j 2h t te/ Initial Dilution Initial Dilution Factor O. /Z2 of Sample diluted to /DO ml with Dl water 3 fcw r (Dilution factor = 1 if undiluted) Bottle Cone, of No. . Sample1 Sample Site3 Initial Prtant D.O. (Di ) D.O.(D2) 22. 10 8* bh !Z 8 .r n 1 .0 H .o sr / k j t e G$ ' >6 W .y o 3 . ? 3 -S'O f .7 r 2.6> < ,r Z .'l'i' < o . r Total D.O. Depletion (Dv-Oa) (,h o c^ X ^-- -5r U JL 4 \ . -* T vlS ujlJ*. Depletion Due To8ample S> -Dev BOD _ Average S Dev BOD r8 H'1<r $3.%00 Z lij O ifff^OO y * 3 % - -* --- -- . - f.tr krUi-trtv . 1C i l Fi*1v1&*- rO^L{ 'nL-- Tt AO *r; ' 5 -g ;;',:3r ,3 5 ..T.D EP , 1 S U o 6 6 ' 6 ? .'? D D S ` 4 2 C C 5 5 ^ f = BDD \3W j000 Note: 1. Cone. = Concentration of sample (ml/l o^mg/l) in BOD bottles. 2. Sample Size = ml or g of sample or diluted sample added to BOD Bottle. Comment oO " ^ rZ0,l5D BOD i E S T - `F -'E S IJL fS ^ " ' : V - ' ' 4. (fip/Gv ' ; 5 3 T D E P ` 4 ;7 5 7 3 3 3 3 3 3 ' DDS -, ' 356. 8 * ^ , BO D '' P^ eA A turyv sp ifo /jn sm till. . X~Vij 6Dt> ~r ' A l t . . 3 .7 mi - / / * s ) % S o . S BDD T E S T R E S U L T S -,;V 8 . ( '-'G' V N L th d L , W ^ d iA J r k h o s rn tL / flP a m L ^ iA Jb cti fy \ -;^ ^ r 4 .- .|s 6 7 :* !& . V: ' 2 1 0 ~55 X-f " BDD Form 19756 PWQ *$l&i: 'm t& '^P:AFH'^A`';AAA: .'] . -'k -..-.w....' Wl " M W# mS ?' Invfrofmwntal Laboratory ^^ * J90 Worte Shaat Sampisi AA''' >r :!r *; t '. '.h ' -:.i.i.V'.''-'-'-.-'5-': Lab Req. No. | ^> g g , V "y. _,y. l: ;V\t __B_*.;y ;;P)lun,/No.>' A S-A A i W M M - - y :,:,+ SI - / '* `F' . .;y-:.V'/: Analyst ^ 4 . A u ^ J7 Dete'Sterted f, yaht Analyst. /A a . ' Day BOD Readings 1 \`.2 lOate. v . U rki F C -Z O jl ' .oT 2 3 0 0Semple Description Initial Dilution o. /z s (8C#~/ ) of Sample diluted to / 0 0 ml with Dl water Initial Dilution Factor /J ./ 2 g (Dilution factor 1 if undiluted) Bottle No. 0 h it J . Cone, of Semple1 $ .r SUe2 Initial D.O.(Dn) z.o : t/lC 11 1 H- r/ Gg H'O ili n IA_ 3 -g 'i< tn : Preeent 0 .0 . (Da ) io .r <p' Total 0 .0 . Depletion 10,-Bj) Depletion Due To Semple tJ-* - Day I BOO Av6' Day BOO GY.o 4 jH - : B Q"A:T fe s 'f R e s u- l t' s-V" -r-je, : , MLyG:;; 6. 35 T IiE P y ' 5. 5 5 t ' 6 6 6 6 ? ' 'B}S"-; TtW; 825. 8 5 $ ^ i n r r _ 1. Cone. - Concentration of sample (ml/l or mg/l) in BOD bottles. 2. Sample Size = m l or g of sample or diluted sample added to BOD Bottle. 7 Comments CoO 'Izo ,tm .:,j`v -''ir-.y3>"' .v'yvs'*'*>. --r"T BEST COPY AVAILABLE Farm 1t 7S PWO mmmmmr .... --iV- v 'jcam m tnti Cone. ' :'^My-^i?tS Sample Size 3 ml or g of sample or diluted sample added to BOD Bottle. K - ;'' ' i >$<1' '-c, V-S-i, ' f*V--> 'i,-.-^y'.'-A*;". ."1L-*-? a -'Q - ^ z o , m - .... . ''- T" its* rSfe- -F o rm 1* 7 5 PW O BEST COPY AVAILABLE .V 4 :.c.--'4; 4 ^ '' v-:X'4h'J-r m n....?. >.. * Bnvtytinnwntal Laboratory BOD Work Sheet Blanks, Controls, and Standards Lab Request No. | g (?fVL4 O at S ta rte d s>, # s u ^ e ^ 3 -1 3 -S ' Saad Typ A S o v m Seed C o n c e n tra tio n . -O ji/A Nonsaedod Dilution Water Blank BOO, Run No. | * $ / - / , ' Day BOD Readings A n a ly s t _ - >v A > . Q. S . l t i P D ata t Botila No. Initial D.O. (A t ) ? Z .S o SSo 3 Saadad Dilution Watar Blank (optional) Praaant D.O . IA j I Z 'H o V -H -o ear D .O . Daplation lA ^ A a l S .io 0 . io 0 - 3 -T Avaraga D.O . Daplation ( 1 : %2l 0 .1 8 1 Botti No. 1 u ff 7 Saed Controls BottiaNo. 3 H s> Initial 0 .0 . (C ,! % S S -8 S SawpiaSI 0 Ho $o io Praaant D.O . (C al f f .x r 5 ?-a s" r-3 o " Initia(Bl1D).O.` at JZ b -- S . S 7 ./ r ^ ssr D.O . Daplation (C i -Cal O SS' > ifr ? .r r v ; Praaa(BntaDi.O. > do 3 ,t> r < o .r < o .r Avaraga D.O . Daplation (S ) - S j) 6S S - D.O(B.D,a-Bplaat)ion 3* b 5/flo '--- <tA Bottia No. 13 J3 3 J2 .0 Sampia Sita 3 .0 V o l.o Initial D.O . (Df) l> X % 1S S . S Praaant D .O . / ' a / Total D .O . Daplation (D ^ -D a l a .# * 3 .ro f sv/< r Daplation Dua To Standard IA l 33/ H<Ql % -Dav BOD _ Avaraga * - S T - -Dav BOD /?/ /? f ? A o o y n o / j t w' Bottia No. Sample Size , 8V / SV x / n t lyXx--------x-------a^-4 Initial D.O. (D nl t-% o K - fa ______d n w d i f r t f 'Ir Praaant D .O . (D j) c- t ~ '' i / g / . a w / m , . nriK,/r-r r r w u i ^ Total D .O . Daplation (D , - Da). Daplation e in * /w e* -Day DOP - &.SO i < 5 .S " 6 f30 o ;r * v O .H l 6 .V 6 - J Jy Dar 00287^ Jtovironmental Laboratory PfWrk SHset -Blank: ntrols, ind Standards p-,-: :V * ' - '-' '-V .": V.V-W SS :. V/.W ' ; ' S. - ^ ' V B: b Request No.# ($ Aftelytt Bead Type & Source V ' Date Started 2-tZ-P Saad Concentration 0ii.MiLt' CUrxtM(t Nonseedad Dilution - Water Blank BOD Run No. # zL Analyvt Pay- BOO Readings =44= : w . T "r--? m Bottle No. Initial D.O. (A,) ? .Sa Sx Z-$0 : 2 3 -... %'S^L. Seeded Dilution Water Blank (optional) PreeentP.O. (Aa) ^ < 5o % .nr t.xs~ D.O. Depletion' (At -A2) < 6 o r c o .v ^ ^ - :^ Average D.O. Depletion (! -2) Bottle N. ; I :- - V. Seed Controls ^ Bottle No. Initial D.O. \ Wti 2 TU o %-8f SempleSite - 3 H *40 : 50 . /4 0 : PreventP.O. a 7 .^ 0 < ?r 6 -^ 0 0 >. ' Initial D.O. IB|I fTc, 5. o r -1. 1 D.O. Depletion (C, -C2> : d? t ^ o " : 7 o .^ iT ' O iE T V O Average D.O. Depletion tC-t -C2I >< I r :a . Preeent D.O. D.O. Depletion (Bjjl , -r#? - V. r r p : W ^ X X :c / 6 >i^ 9 -- -- Glucose - Glutamic Acid Standard Bottle No.. SampleSize Initial D.. 1 )3 3 ,o T rO 1*0 7 o -2.o .'H i' Prevent D.O. ; (D2) Total D.O. Depletion (0,-0^ DepletionDue' ! To Standard . J -Dev BOD r -70 - 3 i 70 <4,-76 1*9>(p X%> cV^fo \) ^ 2> %. <* a -tiT ptb S ` -5 7 : 5 - . 3 ' o J )Llu,&rn jaJIZm* ^t<L-rvfkU .<x/CC=^ Average \j ' i -Dev 33o Bottle No. , >Sample Size Initial D.O. (Dr ) 8 '' sv * mm. y 8 .3 0 & S Prevent D.O . . <2> . 8-0S- t/ Total D.O . Depletion (D fD a l 'j 0/70 V 07 Depletion-Doe ^2 -Day & Uc7 ly ^ e fe d v f Or7 0 .-. ... . -,if -*****. *r ,r ,B ,Oev . PGPD fce;.l V iwirocw*ntat Laboratory BOD Work Stoat - Blanks, Controls, and Standards ; e r ^ y Lfi~ L ib Request No. | Analyst ^^ fly, $ Seed Type & Saure* Seed Concentration C!idy*0(L^i T Nonaaadad Dilution - Water Blank s3> <^c> L it n v r m 2 - r f Zo l i z LBOD Run No. | Analyst A<L - Day BOD Readings SZi . * >': : * j v , -.- Oata 3>- o f - J>/ Betti No. in itial D .O . (A ,) sy s, : V .g o S .fo S -8 o Saadad Dilution Water Blank, (optkxial) ' Bottle No. . In itiaiD .O . t si : ,, -- t : f.y- U S V: V -. .. 7 - : :: Seed Controls : ? 2 ? - ifo Present D .O . (A 2 ) g ,o o S-oo I ... S . - i . f c _______________ D .O . Depletion ` (A , * Aj> o .* O `$0 0 `70 > Average D .O . Depletion lA i - X 2) .* o rn i ,' P re e an tD .O . fc l n .x o y 1 . io ; ..: . -. 1 D .O . Depletion tet -Ca) ^ y--l:m \ r y - f y ' / . S o f ' ' :: y y ' - y n c -- V: Average O .O . . : Depletion ' ' C i "Cj) ' . \ l .... : '.rf.y h it Bottle No. Semple Size y3 n V , s> 40 : SO />o Gluc'ose Glutam: ic A cid Standard. _____ ____________ _________ ---------- --------------------------- -------- BottteNo. ! Sample Size Initial D.O . ID t l 13 ? 0 JAO 7 . 0 S .6 S " o nte No. Sample Size 8\ / "7 !> /X Initial D.O . : ID , i-% 0 X -So S 'K In itia iD .O . (B vl 2-S6 s .o r US' r .r r P re ae n tD .O . (8 3) D .O . Depletion iBt-Ba) A: : r: --- -- l ''-:"- - - -.'7 - 1 : -- T-r-- V 4* - ' v , `' ; ; : IVaaant D.O . id2> Total D O. Depletion I D , ` Da Depletion Due , .ToStandard S ', s o s '.r o `/ J 3 ' 3 r/0 : 7 .ro < ir 3v S " SjutfddL UiwteTi frill- J>4<L*4>aJ Prem ntD .O . K>a> ' Total D .O . Depletion (D t - 0 2) --7 7 V-------- Depletion BernTe Otandeid AOGr . 7^ 6/ l-7 f // 7 /. / r > / r 'Z rt) ^ /OS' . ''L " <A0 -Dav BOD H IS ) 3 3 ,3 > %C> $ J - Day *800^ V Average " ;A- d __ BOO _ O n 340 . -Day . j i * . ; ..... ' y_ v*-kfe '