Document 4J7DnxgeG0QLEZDp52QEGwxqG
AR ARG - 12 7
FINAL REPORT
g
28
32
3
sing 2 erursocchyan) Tes bance oKe s919]rcp Bioconcentrationtestof2-perfluoroalikyl (C=4-16) ethanol [This test wasperformed Clestmamber: 51518)
eo
Chemicals EviKaurnume LiRberasrey sins, ip 0000014
sists
' STATEMENT
RKueChrseumeimceanlLsibEsovraearyun,p1nd
Spon
ASAHIGLCoA,SLiSt.
vBisocopnecetnormaeiodntweisntgof22-ppeefoorrsootnly)(Ca1a6n) c(haayn nThisses
`number K-1518)]incarp
Tetmmer sisi
ofteJFytihenuRnedpeosrg(nTees,tbNeor.eS15de1c,isetdaoniFsrreapoyp3r5ov3i0d0es1.coresEnglish aston
Due StyndDiecir
February 35 2003 EEirnnaeKi[HshPion Rhn
000002
sists GLP STATEMENT
Sponsor nu
.
RCehseerairccthsnEsve,aJnapsand
Minis ofEconToramdyea,ndIndustry
Biosonsenation eof 2pefuoroslyl (Cod-16) chan (This test
was performed using 2<{perfluorooctyl) ethanol (Test substance
`numberK-1518))incarp
Test rumber sists
induTshwiials cehsetmwiacsalsp,eo"Srtmaneddairdn cCoomnpcleimainncegwiTtehsGiogodFLaacboirlatPorroyvPirdaecdtifcne ASarnidcarees4foorf MiT2n0oi0is0tc,ekryRiNaole3o9r,sdinePaoannfcaneDPiernrsegisgcncaabntihdendgCCohToeermsditiclnaaeltmiSosunbRsetBlauantrcieenasgu",t(oaENsnevhiwv3Cm1hm,eem1n9it8c4a,lARgeeSvnuicbsyse,tdaMnNcaeorsc2ha2n,1d, BPhuarmeaac,Meuiicial AoffeiraButrieaou,nMTirasdteyaonfdHnessh,andJWaeaplndafa"nOdneEC,)DNoP.5r,iBnacsiipleesnodfGsood LdaabaoratartehoavrasylPbiredaecnticcoen"f(iNromveedmbhaetrti26s, 1i9n9a7)e. psreflets he rawctsscalyan te test
Due Study Disccior
February2,2000 Signed in orginal Erna Kikushima
000003
sis
QUALITY ASSURANCE STATEMENT
[ CRheoarisi-- Evento Spore MinkyofEcoTsensdon,t
Title
nT `Bioconcentration test of 2-perfluoroalkyl (C=4-16)
was performed using 2-(perfluorooctyl) ethanol
ethanol (Test
[This test. substance
Tamme sie
mCoseTenhsEnpierc0ioxnosSidoFyf inDcitrstewebyeecGrai,eydaestraanedssUroesosfvKeosnsp?aroed 0n
Pros
Geiober 10,2000 | Octtobye 1d0,2e00r0 Novem 5,200
OuTatmaerry307,.21000010
Febru5,a2r00y0
November 2000
Tec
November1,2000| November2.2000
Rovere: 16,000| Nese142000| owes 16300
cits enus crpesnieecctotmthpefslwihprBsepdreossoesseScreht ted te ot
Due
Foran. 201
Qu Asencio Spatinrgn
Koobiorion
000004
sisi
CONTENTS
.
Tide
\
Sponsor
:
:
Testing fly
.
Objctive
\
Tesmethod
.
Applied GLP
2
Dues
,
`Stofotesrtsuabstgancee, rawdat,etc.
2
Pencnnel
.
Approval ofa report
5
SUMMARY
.
1 Testsubstance
s
2rtm tsi
,
3. Perfoofriomcoacenntcaieon test
4 Factorspossiblyaffecting sccrscy
2
5 Ress
& Bele tine
2%
7. Discussion
"
8 Remarks
000005
sisis
Contents oftables snd figs
Contemsoftables
Tabled Meanredconcentrationsof estsubstance nes wale scex)
Tez BCRs(seeten)
Tabled ConcenuaindoBnCFss ofthe etsubstainncael hepars (ee tex)
Tabled Resirtdeuofatelst subindsepurtatioan tenst(sceeteex)
Tables Caluladon ableforrecoveryand bar tes analysis fest vate)
Tables Calalaion ablefo analysis oftestwater(Level 1)
Tle? Callaion ablefoanaofltestywatseri(Levsel 2)
Tables Caledon able orrecoveryand lank est (analysisoftes sh)
Tabled Calculation tafobranlalyesis oftest sh (Level 1)
Tabel0 Calulaion ablefoanalyoftsestissh (Level 2)
Tabled] Calcablle oaranoalynsisoftest sh (Contl)
Table12 Callantable oralysisinpssoftest fh (Level 1)
Tablets Calculbleafotrailyosinsinparsoftest sh (Level2)
[J
Tables Calculation table foranalysis fetish (Level 1) depuration ex)
Table1s Calclaon table or naysof st sh (Level 2) (depuration es)
Referenc|e Anresua ltsofy utiona water
000005
sists
Cones gues Fig1 [Comrelmtonbeoveen xpos periodand boconcenzaftiorn Fig2 Comelaejyonbesween expose prod sad boconeniration coe Fig Conemvaionmoraliy cave
Fig4 M--ass fragmentogram and calibration curve of GC-MS analysis for test
Figs aMasns aagomteneisogiram ofGC-MSalysisforrecoanvdbeakreyst
Fig6 Mas agmentopamofGC-MS alyfosanialyssisof stwater
Fie Masogrmentogrmsnd clbraioncrv ofGC-MS alysisfrst sh
Fig M(aanaslsysoipmseantotgaem s BoyofGC-MSanalysisfo recoanvdbeakrsyt
Figs MassapmenopamofGC-MS alyfoseisfsish (Level )
Figl0 Mass GapmenogamofGC-MS amaforlstyfishs(Lesvel 2)
Figll Mass GapmenogamofGC-MS alfoystsfishi(Cosrl)
Fill Conceommionaf ichvedoxygen(Level 1)
Field Concenraionofdisolvedoxygen (Level)
Fight Concenuaionofdisolved xygen (Control)
FiBgEls MabseEapgnaenttoofgiGrC-aMmS slyforiehsLotve 1)
Figle MashseEngmpentogaramotf GC MSsmlysisforestsh(Level2)
Fpl? M(asaspgmmentogra ofGC-MS alysisforts ih (Love 1)
FIRS M(aespmigmmeinatsosgram ofGC-MSsly for es fh(Lovet2)
Figi51 IRspectrumoftsbsanemessedbefore he expensing
FigI92 IRspecuoftessbsancemesure aferh peincompletion
Fig) Mas spocfetbance
Reference 2 NMRspectrumof stssa
000007
sis i
Tide
1
sing2B-ipoceornvcreonoactiyoln)teetshtaonfol2-(pTeesrtfsuucbrsotaainycle u(mCb1e6r)K-e1t5h1an8o)li[Tcharspest was performed |
Sponsor M1i:s51sRyaosfumEicgoansoem,y,CThriayoddeaa-nkdu,ITnosot 100-6901span
Testing fly
CK1hu9e:rm1ui4cmaehlLisaobmBoavrcsahtuion,riyKoenrsudmeR-essie,arFcuhkIvnoksat83n0,.0J0a2pa3n, span
Objcive Tsewas performedtoeval theioconcenrainpetenial of K-1518incarp.
Tes method ANcocwuTmCihhlestmiitocenasltovfSCauhbsesmpaiencracfleosSmu(ebdlsayacpce1oe3xs,di1in9nF7gi4s,thoRBetovhidesyed""MsOecptthouboeidrfco8i,drn1t9Th9ee8s,"tTiNensogtstin,hgeMPDeletgahrioednesafnoodrf CSTnohenomcatismsoanioaofln THBeausrestan,adndE.InnvdWiusertolrnylm,e,JnatpianA)dg,enNacony3d,9"2NB,ioolBcasso,incsPcIhsnatdmisantciee:ustFilcBoawuleraAo,ufgMhiiFiBnshusTcesaotf (Ooi 305. une 14,1996 ithe OECD Guideline or Testing ofCheicls.
a.
000008
sisi
Applied GLP (0 Chemical GLP. sclThi4sotfesMtinciostmeprliiaeldorwdiitnhan"cSeuPurnedsacrrdibCionngcTeersntinlgemTsesRteilnagtiFnagcitloitNyePwroCvhiedmeidcailn S1u9b84s,ancReesviasnedd ToMiacricthy R1e,se2a0r0c0h,ofNDoes3i9g,natPeldannCihnegmicaaald SuCbosotradnicneastio(nMarBcuhre3a1,, aEnndviWonemealtaAnfgdeNanocy.r,3,NeBoa.s2,2i9cI,ndPuhsatmriiceesuBtuirceaalu,AMfifnsirsstryBuorfeaIs,neMinrismtrayTtorfoadHneeaanltldh Indus, Japan. @oEcD"GThiLsetest complied with "OECD Principles of Good Laboratory Practice" (Novemb2e6r, 1997).
Dates
Study inition date: Experimentalsatingdae: Experimental completion ate Studycompletiondate
October 10,2000 Ociober 23,2000 December22,2000 February22,2000
Stooftrestasubsgtanece, raw dat, et.
(0)Test subsance
biodeBgeracdaautsieotnhteest,etshtsuebssttsaunbcsteoanfcethiesoatmseolreodaifs earlrehaidstysestto.redaferthe endof
2) RawRdaawadnasd,mattheerpiraolt,ocetocl., documentsabout heLestpresentedbythesponsor,the finalreportandnecessarymaterialsar toed n astorageroomin his abortry
2.
000009
sists
Personnel Study Dicir
(SOtupdeyrpeornsoonfnselconeniraion 2)
Suffforfh cae
A
persontoconductoffishsate oxicytest
EraKishima
KYoajziuKmusauFnuejimoto
v
Emi Yamaguchi
Vaso Kawashima
YKaastsuuomKiaKvoasrhoima
@ mews
`Study Director
Date
Signe
February 22,2001
Signedinorignal Erion Kishin
3 000010
sis1s
SUMMARY
Tide sing2B-iopceonucoerntoroatciyon)teestthoafn2-(pTeesrtlusousbosntlainyclem(uCm=4b-e1r6K)-e1t5h1a8n)o]l i[nTchaisrptest was performed
Tet conditions
Acetoxicly tet
(1) Testfish
Orange-redkillifish(Qrziaslatipes)
(2) Duration ofexposure 96 hrs.
(3) Exposure method (R`eSenmeiwstaalticfseyssttweamter, a every 8- 161s)
Bios(a)ncTeensrtafiioshn est Crp(Copiousai)
@)Nomina concentrations ofeHtigshubestaxncepleovels(Leevel 1) 10g
()Duntonofexposure Lowdaeys xplevoel Lseveel) 1 gL
Exposure method Continuous flow system
(5) Analytical method
Gas chromatography-mass spectrometry
res ())B9i6ohcoowncreLnCrstoovnifiecors >Le1v2e1l5mg 200-1100 Lolz 87. 310
"a
000011
sists
1 Testa In irepor,K-1518hasthe allowingchemicalname,ec.
11 Chemicalname 2-{perfluorooctyl)ethanol
12 Chemicasvete,etc.
`Structuralformula
-
CF4--(CFa)s--CF:CH,CH--OH
Molarformula CulFnO Molecular weight et
13 M()aMannsufcftcnaearmce~atn~dulorDtAneIuKmrIbeN,Fri"nechenicalLaboratory,Lid (2O)loNatmmember ~~ AA-I1B82200TX01 "1 Infsuopplmiedabytthemiantoicn
14 PuTiessas 98%(G0) "The estsubsancewsweedas 100%inpity.
5 000012
sisig 15 FCigo19n[)T,fhMeasisisrrousfcmpueraesetcotsftutbi(rshtsuoeanemtcneeF.sitgsu2b0s)atanndceNiMsRcosnfpiermcetdbr(yuseimenfRreafreerden(cIeR)2s).pectrum (see 16 Stoanrdsaagltey
(1) Storagecondition Coldstorageplace +
@fSiunbdiinTlghyethtaetstIRsusbpsetcatnrcaeowftahsetseasbtlseuubnsdtaenrctehebstoeraafgnedcooanfdeririteohneasexspheroiwmnentbywtehree den(teeiFiag 1l9).
17 Stabilityundertestingconditions conditPiroinosrwa0sthceonbfiiorcmoendcbenytraaptroenlsitm,instaaebrstiy.ly ofthetetsubstanceunderthe esting
-6-
000013
sists
2. Perfoorfamcutaetnxcicey test
21 T01e02s"-T1t9h9ee8t.de71s.t)v,a"sTpeesrtifnogrmmeedtihnodscofrordainndcusetwriiatlhwJaasptaenevsaeiIn,duAsctuitael oSxtianidyared(st1SwitKh fi
22 Tesfish (Species
ORranegefscoerdtskeiloleicfiniohn (:rOTenhazidisialsyapavegaceislsai)bslseimailteasrtifshe.nsitivitytocarpand
@
SuppOligerawashoten (Address 181
Ooish-machi
Kurume:shi,
Fukuoka
830-0049,
par)
(3) Caonbdi"ntTioooenfsrifswomhfewrfariesrheleccmiahoeevtcbekedyef.odrvTeihascecalfliiymsaahttiwzteahtreieornreeceairveidnfgoan2d3tdoasyesidenmoansltorwattirnogaungyh `System following an extemal disinfection.
(@) Caocncdiim`taAitofinzserfdeostthiaencrcgel,itsmahhteeifzhtaihitssihpoenswrceicorodendwterexaentsrefmeeamrlroevddietsoiananfedncttaihocnel.riemmaTathiienzdifenirsghoafqduehameroinsushtmmwaaeinnrdge `Tmeayrheswefhroeorc4rh0etdcyakyesdifronirng8hefallotwh tcroonudigohnssyastnedmreatartehdeatneomtpheerra3t4udreaoyfs2a5fr2theCe.xterTnhael
disinfection.
wege wege 0303
@OLengh ovenge diem 7) CeniiationhourLCS0 valeoftheerence substance." o hefhoftesameot
(TFO-000801)was 0.556 mg +2 PCP-N (peniachorophenolsodiumsal, Tokyo Kasei Kogyo Co, Li)
-7-
000014
sists
23 Dion ater fortes
originGroundwater om the premises ofKurume Lborsy.
@) WaatlTeyrhzqeedudsanidytmiaoesnswaaetdnetr(fonocreetvesetrvysasxtmaoknetnhosuitnohnis AMubosrtio8,n2000T, aon miewass
MhoiwnniItsvinaoRseffcHeoerafelnitchmeea1.dndthWtetlfearelu(tDoencembteer m21e,tt19e62)i,iwshterrlquairlitasnenosf phey
(GfGuisuihidederelilieinsnees(S2hf1ao0d,aTbJeosyziinng17,Nof1v9C92h)eSm3ii0csdanlie,gnev"inFoinshHu,onegEtoaulltyyoakllsii,ftyMSapsuenncTa1ro5t8s,oirOwETCerDa) polluans No. 14 (Revised February 2, 199, Environment
(GuGiuddeeliinnees3f0r5Tnesein1go4,f1C9h9e6ricl, "Bioconcentation: FlowtAgoeungeyhoFrebOeTeyo
24 Testconsiions
(0) Testank
Glas gallonvessel
@Volumefiestuser 385Lxe2ve
Temp"eArtaatleoefxepsotsweer Beforerenewalcfistvaier
u2s41c.242C
o
) Co`BeAftonrriearlceenoxefepwoiansssleoiltvsedtoexyrgen nes5St2ow.m3ge7Lmg
por`tinsdaeteerxpose Beforerenalafter
177s
Numberor ch 10/1evl
O)Duionofexponre 361s
OFpocemetod RSeemnieswsalyfesmt tea ev,ry 61s)
5
000015
sisi
25 Prepaofrstaocktsioluotinon
(1)Dispersant HCO-20 (Hydrogecnasatotreild)
2)Prepucation dis"Tshewoiestlhsavucbetseotnaden.ceanfdeHrCtOh-ea2c0e(t4o0ntewimaessaevmaopuonrtatoeftdhfertoemtthseusbosltuatnicoen)weforne`exchangedwaterwasaddedtothemixture to prepare 1000mg/Lstocksolution.
26 Performaonfceset
(Pace 2141050 room
@Due `October 16, 20-Oct0ober020,2000
27 Estiof9m6-haoutrLCiSOovalnue: `The96-hourLCS0vahuewasestimatedbytheDoudoroffmethod.
28 Resutofest ShowLCSOvalve
> 125 mg/L" (see Fig. 3)
*3 T`heacocncoecfnttrohaetituoonxniociftttyhoefdtihsepedrsianstpatetrhtsihsattneistmteawtahisghSe0r0c0omgn/cLe.ntTarkawinatgsiinnotonot
performed,becausethe 96-hour LCS0valueof he gL.
dispwaesmorresthaan5n00t00
5. 000016
sists
3.Perfoofbrocomnceantrnatconeest
31 Tesh
(1) Species
CRarpe(Caufporrsisenluoescctaniropni:o)Theprevious data conducted withthisspecies + aandbeecoqmfopurahraaenddtalniendgthesizeoftisspecies
@ SuppliFeurlcuokaken yabegawa fishermen's cooperative association (Address: 748 Yamauchi, Yame-shi, Fuuoka 834-0012 Japan)
Datereceived Septe1m,b2e00r0
0) Conctareierscncmmiozion "Thefish werecheckedvisualyinthe receivingandthose demonstrating <= abnorwmeraerlemiovtedy. Thefish were reared.for 8daysin a flow throu system following an extemal disinfection.
(4) Condi`tiAotnesrfeoarrisnegc,ltihmeaizfiasthiownere medi10celaimiinadte parasites and transfered > an acclimaiizing aquarium. Afer the second extemal disinfection, they were acclimatized. The fish demonstrating any abnormality during this period were sryesmtoevmeadtatnhdettheermempaiendroerfao2ft5:uth4er2fiesCh. wTehreereisahrewdefroert1heSn aryanssifneraefdltoowtteshtrtoaugs andrearedatthe sametemperature inthe lowthrough systemfor25days, follow'=3 the extemal disinfection.
(Length 65-94m
(6LoNo. TFC-000901
(Age Yeung fish
()FesdFinege. d
Feed for fyofcar
Composition PLrpoitdeicnosncoenntten~t~. ZZ 433.00%%
Manufacturer Nippon FormulaFeed Mfg. Co,Lid.
Feed`iAngmoaumnotucnotrraensdpoinntdeirnvagltoabout 2%oftotalbody weightwas fedtwice 2
dayi"nThhaelfviessh.werestarvedfor 24hoursbeforesampling.
-10-
000017
sis
32 Diohnewd sae ottsinst ein23, 53 Confdsindicioumsntscses
(1) ow arogys Sup oftpestlwatyer sme i his boron vi id.
(2) Te 00. sttank gs for olatlr - bsance
0 iFirowareedosrifvtwoiasrrockrssaWu ALsnd 1600minfor iinvat ;2507
(Sok uicabotls F1e0ses lebtgreCeolin3inmeeigeen
TeTLmoedzptesetvr"et3s530s.225C6
Cows pa3s6c
( Concenenisfdislodge vate
Level 1
76mp- l(s7 eeFig9 12)
Level 2
77mg- /L (s7 eeFig9 .13)
Control
78mg8 /L(s. ee Fi0 g14)
pHoafavntai fear
1766.177
Cool 1678
( TimeR ort ovfwihge org s ap1hs)
(5) NumCbew orooflga he ei3 nof xp)
(0)DuinoteEpeotenheion: TShi0ei8nnfo3rdeaaycsh Bsoemnrdysmtevas ctrexnti.aed 0
pie
213 Aquronroom
ne
000018
372 Pretri
(1)Test water
ietsP_cotv, ont `rspsecttarokmeentfryro(mGC-aMcSh)taenasltystiasnaks,afnoldlopwrsetestd forgas chromatography.mass
: efrrciestcimtd.
e m -- ae mbte |
y Eir mrreanaiiota nomGEDo,mei ns + Filtration and dehydration (20 mml.D. column,
000019
sisig
*5 Condiotficoolnumsn chromatography
SepPak Cin Loading Elon
WEhlouelnetvoluAmceetoofnitwhlees/opluuriifoinedwawastleora(d4e1d.VN) 1mL
`Test substance was elted with te loaded olution and eluent,
-16-
000020
sisis
373 QuiThteesaamnpllyesssffoorreGsCt-suMbSstaanncaelysis in prevestment were analyzed by gas tche choncevntraotionmofthsaepetcerstotmseuotbrsytaugnncdeeirrntthheasfaomplplloewtionxgaGenaCl-ymtMiScaaalnacloynssdiisfoesnxsc.edeWdhtehne radnigeedofthe caalbcroantcienotnrcautrivon iwiebtifnishtahnealryasnigsethofsatmhpelecwaalsiwaantaolnyzecduaefe.ritvTahse bcoansciesnotrfaticonomopfatrhiseotnesotfstuhbespaensckeianrceaaocnhstahmepmlaesssofuatgomennwtaosgdreatmeormfitnheedsoanmptlhee solution withthatof astandard solution (ce Tables6, 7, Fig6 and Tables, 10, 11, Figs, 10,11),
0)AralyItncsatlrucmoenndittions
GSahsimcahdrzoumaCtoorgporraapthi-omnasstsyppeecQtrPo-m5e0t0er0
`CColoumnnodfgiascthriomoatongasgh 30mx025 mal. Fused silica
ColLuimqunied mphpa.se
H50PSCM@S mfii~mnt9h0i)ckCness--022050yCm
TInejmepcteironautermep.rate
42*C0imin, 30Chnin
Interface temp. Camiergas
H2e5l0iuCm
CTooullufmlobweaadtepressure 4100KPmain
ISnajmepcltiionngmteitmheod
S2pmlitness
Sample size
fr
ain te Ly
MIoonniiztaotriionngvofolnage
ev wiz9s
ae
000021
|
"
2) Preparationofstandard lution the sa"mTphelestsaolnudtairodnsswoaslprtueopdatreetideraosmifnnoleltohwesc. oncentraotfitohne test substance in
(8) Analysisoftestwater MBLs1o0l0umtigonooffthtehseutbessttasnucbeswtaansced.iss1o0lv0edipgn/Lesthtyalnadcaertdastoeltuotpiroenwpaarse t1h0e0n0 prepared from this solutionbydilutionwithethyl scetae.
() Analysisofest fish so1l0u0omfttghioeftotehnsetsusubbssttaannccee.was1d0i0sspogl/vLesdainadcertsoolnuteitoonwpraesptahreen1p0r0e0pmagr/eLd from this solution by dilution with acetoitrile/purifiedwater (4/1 VV),
) Calibration curve.
(6) Analysisof est water
me50ta0s,dhe1s0co0riabdnedd
200 g/L in (2s).
sTthaensdeasrdolustoliuotnisownserweearnealpyrzepeadreadcbcyotrhedtoisantmhgee
baansailsyotifcatlhecroenldaittiioonnbsedcewsecerintbehdepinea(1k).areaAocnatlhbermtiaosnsfcruarvgemewnatsogdrraamwsannodntthhee
respective concentrations.
testsubInstcaonncseidwearastrieognoarfdtehdeasno2i0s0e,lewvheil,cthhcomleoswpesotnddeetdetcota2b.le7ppega/kLoarfetahoeftethset
substance concentration (seeFig.4).
() Analyosftiesst ish metho5d0a0s,d1e00sacndri2in0(b02)egbd)L.sTthaensdaersdosloultuitoinoswnsewreeraenaplryezpeadraecdcboyrdtihngestoatmhee baansailsytiocftalhceonedliattiioonnbsdeetswcereinbtehdeipnea(1k).areAacontahemlasiscfubrravgremewnaatsogdtrraamwisnaonondttnhhee. respecItnivceoncsoindceernatrtaitoinoofnst.he noise level, the lowest detectable peak areaofthe tessubtsstuabnscteacnocnecewnatsrraetgioanrd(secdaFisg.207)0., whichcorrespondedto4.2 pg/L ofthetest:
"18. 000022
sists
374 Recoidvekreyt
(1)MbetahonWdatefroanrdefeersuype6oVf6eehr1e0gdspoikeedspnecwiaeyd admoeutn eofUine3.7e2s. t TAheeSheavvtewhndsSospeersmewedsiperamendmianndneorlwei.ht he bse,
with d nn a-- k ofhn temhss nrgtiosa gsrmoofnGC.MShTodeoopsptfvisegy 1onsd 8nd, 8.sohefaveeryiebcovrieyvieenwaats etdhsoos menicrTs f5h Semin of ses meio 8says mls
FraTiasrostweae n(pwt bcs304wi%de)
[aU ve Rina-- ne " 3%
325 UipidcoTnitedticnosnts nthe sample fo lls ofpi cone of Lev 1, Level 1 1d he col tt Tue 3730 wee dein she Choro bal
`ext witrh graavicmettriciano alysnis.
000023
sists
326 dCaaelmitnoainonof th tet substance concentration in sample nd. misma lit of 0)CalTuihoecnqufahieon eisntTabsltaensc,e7ownascaeedn1iaotiesntnittheenrconcentrations,andthey ere rouofnfod3 fiegurdes.
(2)iD hfoelble oa"wTss.ht seodfeectlhoairr tmaiattbom iafoitnnhi eeitdmefisn ot?smuofba tshthaeet ntcceaesilti bnsrtuaebsio stotnwaann tcceeurrivteiesnwsaetceorwnas3.c9a3lc(lXtoed)oan6
LLoeedi]z
00033
gl gL
(6) CheayuwtTeihreeoncrofqothueusoesiftnnifsTuadobbstlneaiengsscu,des1o0.n,s1emwteironsiedtestbsahinthconcrtonan,d
(6)DeAtsumeingrmtihtemoffintiehestanemsptlsaebsofatfncheintoeobsetn5s,hthdetermination lic"of he testsubsance nest fhwas calcltdtobe 26 ng onthebss ofht canes
fromthecalibrationcurveinsection 3.7.3 (3)b).
*5 Minimumdetermination limitofthetestsubs(tug/aLonfncg/eg)
g3,obCoE
100 * 7D
wheAe+ Minimum determination iit ofthtestsubstance onthecaliraon curve(a)
B::RSaempcliongvrvaoetleur(m%ye)of test ter (uL.)orfiesampleof ih 1)
DE::RFaitnailoovofltuhmeeoorfiosna,mupsleesdolourtaionna(lmyL)stwhol volume
Resultsweeroundedoff02 igure.
20. 000024
sists
327 Ceaxlcpuolsatei)onofmean concentrationofthe text substance in test water (duration of
Tote (Co) + + +Cwl@)I)n
heTroet Themeanconcentration of th etsubstance itestvate(gL) BCnW(l) CoNnucmenbterraotfiaonnaloyfstihsefsoersesstuwbastaenrc(emineIstuanraleysmtieisomnefstt)est we(gL) Ce) Concentrationofthetssubstainnmche anaoflestywastei(4s1)
378 CaBioclonocfeebniroaclionocneanfatcrtaottri(onBiCcP)oowr(sBnCcFa)lulated as fllows.
(1)C2a3u3tionofmeanconcenationofthe estsubsanceintetwaterforcalculating
CCWom= ( (CCw+Cw C(uoor)n1)))1+22.CW()(onn)l+y}/s3ta(nSoam l2onfydaesstniiassh)lofyesstiis)
heTrHe Themean concenmion ofthetestsubstanceintest water for CW) CocncaenltractilBonCaFoif(t4hn9e1tge)stsubsancein rthanalysisoftestwate (g/L)
@)Calculation ofbosoncentaionfactor
BCP --oo-- n
o
hy BCE Bioconcentaion cor
Cf ThCemoenmcconcetehnrntueostnvsuobsfatatnhiceentoeesttnsfubhsotanncfge)in ext waterfor
clcuBaCFi(1n91g)
"ae
000025
sisig
379 CalulableBCF
a 376 (n 9,heBObaoFsfictsahoenfebsheesiucnbbisiitnaendcwedhenteeesnrtmwiBanatCteEirocnehxlciientedeosftStoeemteflsoltlslhouewbiasngltyazneT cide scrion
was used tocalculateth followingcalculable BOF.
sume
LLeevweil21
28 30
3.2.10 Calcuolf apitdcionotennt
Lipidcontentswerecalculatedscording to thefollowingequaion.
Lipidcon(4)t=(eT nTo)t/ x 100
where
To 3
WWWeeieigghihttogoofffhvfsientsmespesle(agf)ormrapakveilmoeeutticoarnaalnyasliyssics oofnitdincovnetsese5l)
38 Treatment ofmerical values
(3
Ofvfetl0o33etdaSfsoonwrcecneiatriolounnivdaelvduaeossouifetdchwcoitretdthasntucbersowtuianntdciheniJgInoStfeZsoftn8v4ta0he1r1wa9aa9y9doffitslheemBs. eeThreceach
figures. BCPss vawlereuroeundsedoffto 2 igure.
nn
4.Factorspossibly affecting accuracy Noadverse effectsonthe elabiliyofthistetwerenoted,
2.
000026
'
ste 4 CTTrEooeERmiia nTssTeShs aaetu m wesorhooh
TS A ----
lt | 2eeA |42 5|r]2 |5se E=E (Unit:pg/L) a os
--u ----m dnta ewoiegbamid.e [i]2T=0rT030mT0=,[aE[0,T2RsT[ropoeeve|
=SinToe [Eom l%[oe [wum e| .a mwo lmTm (80) (550) [eoo w[mw e[wm (420) (950) (7180) (640)
(150) (160) (210) (150) (110) (170)
000027
sists
53 CaCeRiiscogna(soaafesBsiCbiFmesnvalevsienaasof0BdCF(s30Ce(3F,vi)Ce euese) orcthrrte mcieaS ys inian) n pido xpos
54 LipidTcheonctsriinpsid contents nee hac shown ss los BeAteoirtnermaivtaiexopnodsosfe 3492709%4
535 RessNoofscbsneomhmlosyeirnvbaioneoarspvecisvoas nrod
es
61 OmbTjsshiotvseeteassnw1edeepteerrfmoirmnetdetodcilsbrutohnecofleassseoproecesnoof heessbsnce
62 Anyssinofptrs
621 TetTmheebToorhwhichwesexposed or 4dayseresprein pas gum,
PhaCoetnoasdsmi,wisvtiiseesdcooeeffrciahmaeninnasdeklrdieixxmnacieonnpitsnuhgxeceamedap,stttseesarrliwsem,riiaea,wnesicghm3uednseTphuceriaely,siToheniTnytoegmumeenst
62
TReBouCsdswa0s3 piney
ciugrletatacchoerdcionmgetnoitveiocnquosifontin
Tsabtlaea,n13.2Tphrrespewnee
thheeTbmIehnaaceinafoosiffene hohafee cwlaidtegiehotfcnniahef,re(tchteiCpoacnerso3c,7un6nc0i.eonnooffiieonst3s.7b.s6n0)cveawsacsecioeddwiongn
TacCso,ncencaions 3 BCFLofth essquesncysseintalel peomsme,
ae 000028
E EE r = d w C--N --_m" EYEY
i" emtme e3 woe 22 |B
2
<462
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5
<520
v
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000029
sists
5 Depumiontet
631 Tetsetrfmienrgtoeedmte1stn6htweeesexrokn(ho6u4 day, ianscce ee ro y stvssadby
TestcToesntin Flow teoftvar
101600.0 l lsaifkrodrn iooan sw bsan3c5a e0 err y x
Teemu zistewratoewee spp.
632 ResidRueaslircaaelof ieeoftthseetbesstnscubestance i he estsh weresleds lls
.
eX EVY x10
PED Resuloftthe cst substain st Faron msn En Aofbtshoilsutteestq(%u)iof tecstsubsiancon aySomsao i CWF WTtehset(enagve)irageescgoohh)ncethnestesuabacieiotstnfitohonf60
daysfromstartofthistest (ng/g)
"26. 000030
sisi8
633 Re"Tshieresidual rates(%)ofthe est substancei test fishafter 2and7 days fromthe in(tiwtoiiohn)offdtehputreasttisounbswtearnceecialctuelsattfeisdhbayfceorm6p0ardaiysson(wseitThabtehse-a1v4e,r1a5g,ecFiognsc.e1n7t,ra18t)i.on
Tabled Resatieofdtestusubastanlce.
(Unie: %)
Aer
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087
2
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2m. 000031
sists 7. Discusion waisrconAsfidre6r0edtcatys,htheecvusaionf cohfanBgCeFoifsBoCvFer2n0% ninadysifcasereThieiss.hang
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a 000032
sists
& Remarks Insects spies,pci sparse nd ages, or oe es
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am f meAake
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000040
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7 tresmtn 00
ary26,200 Nene A. Fyyluce
000041
Table-ll Calctabulefloanaalytsisoif eostfnish (Control)
Tae sists
Sundard 100u31 Beforete peril ts.
Before he exprinenal sat
wn
at - 2 .
a
-
sa
.
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(bssspl)
J
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1-210
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SwFeit
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000042
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000043
Tables Calan ale or nly pacsof
(Level)
Te sin
fA n D akw
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am
ttTtoogmmar+ 1 woo . ew Cam
tsinwtt+ ng Ht
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February 5.2001
Name KFyioho
000044
Table-14 Calctabulefolranaalytsisoifteostfnish `Depuerst(aLetveol 1n)
`Sunpledescription
A 0) o
Teave. isis xM
Santa 10431
on
Tetfhate?das mo
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on
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SaTnedSaate10r0?4d1ora a0e ' as .
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L: AKveera(gPecXoAnc(m1r)io/Af(st%4b)c/BexDtxrC/hFGax-t1Ee0)00days 1700/8
M:RMeeK/sLax1l000e)
P:Concestebanrceitidarndsooles 100491
Sergi
Febery 6.2000
Nene Kl Ligicts
000045
Table-15 Calcabluefolranaalytsisoifeost nsh. Depurtesstt(Leiveoln2)
Sundard 100g. TTeensfbiiahatteerr22ddoorb Sunder 100431 TTeextffbhaatteerr7ddany
wn
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0F:iWleeigohwoyffmaes sempl0e50%)
1K:CK=o(PXnA(cofte)ta/bAtt(ssr)n/eaBtxosDxenCg/)FGx-E10}0
LM::RAvergsecaonles0em4u)ionofetmbsace fnttfbr 0ays 150g
PCM=oK/nLxc10e0esosumbsaebissaondanrdsoltfon 100g.
SeeFigs
February6,2001
Nome K. Fgjlugto
000046
foferce 1 dnalycical rosulcs of dition var
Semling tae Augus8t, 200
Te te [oprsed slid
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Date : Oot. 4.2000 Meme :K,Fujimoto
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Date Dec. 26.2000
Name 21
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