Document 4J0eD24OLn5n1kJj9L3V9GeYN

A R 30 - O 38C MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-120, or 3M Sample J2905-1. (1-Decanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heneicosafluoro-, ammonium salt, CAS # 67906-42-7) Remarks: Test sample is a mixture of the test substance in water and butyl cellosolve (approximately 25% test substance, 37.5% butyl cellosolve and 37.5% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test sample was from 3M Lot 164. METHOD Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1992 Species: Photobacterium phosphoreum Analytical monitoring: None Statistical methods: Microbics computer software package Test organism source: Microbics Microtox Reagent Test conditions: Per Microbics method Replicates: Two RESULTS Nominal concentrations: 125, 250, 500,1 000 mg/L Test Sample Element values: 30-minute EC10 = 79 (65 - 96) mg/L 30-minute EC50 = 327 (299 - 358) mg/L CONCLUSIONS FC-120 exhibited a 30-minute EC50 for Photobacterium phosphoreum of 327 mg/L. Testing was conducted on the "as-sold" mixture of the subject material in water (-25% subject material). The values reported apply to that mixture and not directly to the subject material. 000162 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY ___________ R elia b ility:: Klimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions: and determination of the sample purity. Because a mixture was tested, care must be taken when interpreting the results. The solvent in the mixture could have enhanced or restricted biological uptake and/or toxicity of the fluorochemical component. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number J2904, 4/20/92. OTHER Last changed: 5/18/00 000103 'm<0U0* 0*vnO m iC R o T O C X Sample Description X-L-13D l1 , Lot M Environmental Lab -- Work Sheet Lap Request No.. Page __\ ______ of _ i Q _____ Date: / d Q f i d ______ A nalyse . K v J ____________ Q w p 0 0 (rrcy c^- *ryV^u Tg VSt w.Tn *- `t Q vT l^rr.ay. oU - "7-7__ - Ar>>vS7iO Tn jn (p dntxn tfW i ~0 Vs IPC r^S. flOicct " Q yO G srQ V L tOT ' P ty ? g y r Q x . N > . ( J TC \ Q * jB. uP S A ~ * ,t fttyi/ytAf'ct < t<st y s >_u.wt c ^ c T t \\CT r^ l <-**** C - ^ ^ V BEST COPY AVfllUtBtE O O O lf r fr FINAL RESULTS 'NUMBER CONC. 2 5 0 .0 0 0 0 4 250.0000 cjr 5 0 0 . 0 0 0 0 6 500.0000 7 1 0 0 0 . OOOO P 1 0 0 0 . OOOO tC 5 0 = 1 6 6 0 . 7 4 2 7 m a / L -J2 9 0 4 - l . R 5 GAMMA GAMMA E S T . *<. E F F E C T O .1277 0 .13 15 0 .22 87 O . 2920 0.58 36 0 .57 22 O . 1271 0 .1 2 7 1 0 .2 6 8 5 0 .2 6 6 5 0 . 5 >7 . 0 . 5o7- 1 1 .3 2 4 ` i.o : 8 . s 11 2 2 . 0 3 2-6.8-60 3 .3 9 4 <95*/. C-ONF IF*EMCE PAm &E : 151 <?. 322:* TO 2 0 6 9 . 7 2 -e> X f i f T o u c h SP A C E -B A R t o c o n t i n u e -------- . . .GL i i { CC i C C t C t t Ci : i 000165 M I C R O T O X D A T A RE F'DR r R IL E NAME: J 2 9 0 4 - 1 . K Sam ole D e s c r io tio n : TEST DATE: TEST TIM E: F C - 1 2 0 LOT 1 6 4 YELLOW L I Q U I D : TEST CONCENTRATIONS ARE CLEAR. COLORLESS P r o c e d u r e : BASIC I n i t i a l C o n c e n tr a tio n : 1000 mg/L T est rim e: 5 m inutes Osmotic A d ju stm e n t: NaCl D ilu tio n Factor : 2 C o n c e n t r a t i o n U n i t s : mo - L NUMBER 1 0 /I CONC. uAMMA 1 8 4/ 48 1 2 5 . OOOO 8 3 / 49 1 2 5 .0 0 0 0 80/ 43 2 5 0 .0 0 0 0 4 8 4 / 45 2 50.0000 5 75/ 37 500.0000 6 8 1 / 38 500.0000 7 8 1 / 31 1 0 0 0 .0 0 0 0 8 8 3 / 31: 1 0 0 0 .0 0 0 0 0 .0 6 0 7 5 0 ."02673* 0 .1 2 7 7 1 # 0 .1 3 1 4 6 # 0 .2 2 8 6 7 # 0 .2 9 2 0 4 # 0 .5 8 3 7 9 # 0 .5 7 2 1 8 # CONTROL I T / 10 = 5 5 / R2 BLANK R A TIO = 0 . 6 0 10 ZDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDPDDDDDDOPDDDPDDDD? 3SL0PE = 1 .07 86 3 3 63 A3 M3 M A3 3 7 T_T7 7 z z : jLZ ZZ Z Z ZZ z z z z z 3 .7 ~7 33 3 S 3 3 *t 3 3 * 3 36 3 35 3 3 .EC 5 0 3 34 3 . 1 0 QDDDDBDDDDDODDDDDDDD^DUD DDDBDDDDDDDDDDB^DDDBBDDBPBDDDDDBVDDY 100 1 0 0 0 CONCENTRATION 10E4 lOEt EC 5 0 = 1 6 6 0 . 7 4 2 7 (957. CO N FID EN C E RANGE; 1 3 1 9 . 8 2 2 0 TO 2 0 3 9 . 7 2 5 8 ) 000166 # Used to r c a lc u la t io n * I n v a l i d aammas S T A T I S T I C A L R E P O R T F O R DAT. F I L E J2904-1.1 E S T IM A T IN G EQ U A T IO N : LOG C = 0 . 9 1 2 9 :< LOG b + 3 . 2 2 0 3 NUMBER OF DATA P A IR S USED: 6 RESIDUAL VARIANCE: 0 .0 0 1 3 9 957. CONFIDENCE FACTOR: 1 .2 5 8 3 1 FOR EC50 CORRELATION CO EFFIC IENT: R = 0 .9 9 2 2 9 EFFEC TIVE CONCENTRATIONS ( 9 5 X CONFIDENCE RANGE): EC 5 o --16 6 0 . 7 4 2 7 m q /L EC 2 0 = 4 6 9 . 4 9 9 0 mg / L EC 10 = 2 2 3 . 4 6 1 3 m q /L EC 1 = 2 5 . 0 3 4 9 mg / L C13 1 9 . 9 2 2 0 4 2 4 .7 4 9 3 ( 188.4842 < 14.7624 TO 2 0 8 9 . 7 2 5 8 ) * * * TO 516.7341) TO 2 6 4 . 9 2 9 0 ) * * * TO 4 2 . 4 5 5 6 ) * * * * * * EXTRAPOLATED RESULTS 000167 P-3 * 10 F IN A L RESULTS J 2?04-l . K 15 NH H hi N /'//H1Hi-lit II !1II////M-I111-I1it r!///;1HH ilitiH IH rfrV IH H I-fitilllH ililM S -iilllH H H H H H riii `IH HtlH H H H H IlH hlM H ilH H H M M H NUMBER CONC, G'AMMO GAMMA E S T . % E F F E C T I 12 5 . <1*000 0 .1 6 8 6 'T* 1 2 5 .0 0 0 0 0 .1 1 7 5 T 2 5 0 .0 0 0 0 0 .3 3 5 6 4ET 2 5 0 .0 0 0 0 5 0 0 .0 0 0 0 0 .3 4 8 4 o .9564 b 5 0 0 .0 0 0 0 0 .9 8 8 6 7 ' 1000.0000 2 .7 5 6 3 S 1 0 0 0 .0 0 0 0 2.84-90 0 .1 3 3 9 0 .1 3 3 9 O- 3646 0 .3 6 4 6 0 .9 9 3 1 0 .9 9 3 1 2 .7 0 4 6 2 .7 0 4 6 1 4 .4 2 9 10.511 25.1 26 2 5 .8 3 ^ 48.8 86 4 9 .7 1 4 7 3 .3 7 8 7 9 .0 1 9 EC 5 0 0 . 9 4 9 6 m q/L (95% CONFIDENCE RANGE: 4 6 3 . 6 8 4 7 TO 5 4 1 . 2 0 9 4 ) 11MMMH M!1II II II H H1!1!1l i H // IIN !!HH11H11i '// H !1,'<<7>!HH /' i<it HHHit H ` 1H ' ><;<n a if a -i n H HH H 111H 11MMH H H M H H H H rfH HHH C f f T o u c h SPACE BAR t o c o n t i n u e . , . . f f L f f C t i C i i l t C r i f r l l l CLCCCCCCEl ECCCCCCECCCCC 0001G8 s u o p e in a ie D 3 0 + pasn # 6 `J t O O O < * 6 0 2 - 1 * 5 01 Z * 8 9 * 2 9 * 3 9 N y a 3 3 N 3 a i 3 N 0 3 */.S6> 9 6 * 6 * 0 0 5 = 0 5 3 3 30 T *301 N0I1VM1N33N03 0001 00T A a a a a a a a a a a a a a a a a a a a v a a a a a a a a a a a a a a a a a a a ^ a a a a a * a a a a a a a a a a a a 01 * >' - V -V~ Z o-v-y.^s..^- i* O in 0 1 3 I * !? 9 N. J y ' ' w 8~ w c y 9 ~ tr5 * * *I = 3 d 0 3 S aaaaaaaaaaaaaaaaaaaaaaaaaadaaoaaaaaaaaaaaaaaaaaaaaaaaaaaaaaz 01 * zi* * o = o n y y *Nyna *206*9*2 *62956*2 *29886-0 * 0 * 9 5 6 *O *T*8*2` 0 *ZSS22 0 *9*61T -0 *29891*0 0 0 0 0 ' 0001 0000-000i 0000' 00s 0000-00s 0000*052 0 0 0 0 -0 5 2 0000*521 0000*521 26 /82 = 01/ l i 3Q31N03 6 /2S 6 /18 L I /IS 91 /S Z 92 /*8 52 /08 T /'8 02 /*8 8 L 9 5 t? z I ywwy9 --1/ Dui : s 4 t u n u o i ^ e j ^ u a d u o j - : joir-e-j u o n n ijQ I3 N : 4 u a ii'^ s r.rp y d t ^ ouisq *3 N 0 3 11/01 33awriN 3/ DUi 0 0 0 I s a ; nuTUi g j :ewTj_ } s e x : uot; j ; u3 3 oo ie i;T U I 3 1 8 0 3 :a jn p a d o jj SS3330303 *30333 3 y y s N 0 i i y a i N 3 3 N 0 3 i s s i airain m o u s a * 9 t i o n 021-03 ~ :3 W I1 1 S 3 1 :31a 1S31 :uT;dTJ35SQ sjdwes S Iy T- * 0 6 2 f :3W0N 3 1 1 3 130333 yioa x01033iw 01 p <3 STATISTICAL REPORT FOR DATA FILE J2904-1.K15 E S T IM A T IN G EQ UATION: LOG C = 0 . 6 8 6 1 x LOG ^ + 2 . 6 9 9 8 NUMBER OF DATA P A IR S USED: 8 RESIDUAL VARIANCE: 0 .0 0 1 2 5 95V. CONFIDENCE FACTOR: 1 . 0 8 0 3 7 FOR EC50 CORRELATION COEFFICIENT: R = 0 .9 9 5 8 5 E F F E C T IV E CONCENTRATIONS <957. CONFIDENCE RANGE): EC 5 0 = 5 0 0 . 9 4 9 6 mci/L EC 2 0 = 1 9 3 . 5 1 7 5 m g/L EC 1 0 = 1 1 0 . 9 3 9 8 ma / L EC 1 = 2 1 . 4 0 8 5 m g /L ( 463.6847 < 176.9699 ( 97.6970 < 16.4163 TO TO TO TO 5 4 1 .2 0 9 4 ) 2 1 1 .6 1 2 4 ) 1 2 5 .9 7 7 7 )*** 2 7 .9 1 8 9 )*** * * * EXTRAPOLATED RESULTS 00017 P*,. * O F I MAL RESULTS NUMBER CONC. i 125. 0000 a 125.0000 3 250.0000 4 2 5 0 . 0000 5 500. 0000 6 5 0 0 . 0000 7 1000. 0000 8 1000. 0000 EC 5 0 = 3 2 7 . 3 1 9 2 m q/L J 2 9 0 4 - 1 . K-30 GAMMA GAMMA E S T . 7. E F F E C T 0 .1 9 1 6 0 .2 2 6 4 0 .7 7 3 2 0 . 6550 2 .3 2 4 7 2 .1 9 1 7 4 .7 4 5 1 4 .8 8 7 0 0.23 06 0 . 2306 0 .6 6 3 9 0 . 6639 1 .9 1 1 8 1 .9 1 1 8 5 . 5052 5 .5 0 5 2 16.07B 1 8 .4 6 4 4 3 . 604 3 9 .5 7 6 69.9 22 6 8 .6 6 9 8 2 .5 9 4 8 3.0 13 (957. CONFIDENCE RANGE: 2 9 9 . 1 9 0 4 TO .0926 C C C C T o u c h SPACE BAR t o c o n t i n u e ------- C C C C C C C C C C C C C C C C C C C C C C C C C C C C f C C C C C L C C C C C C C C X O O O lT l 5UOT^H>iri3xe3 j o i pesn # S i/O O p < 9 S 6 0 '8 S 0 1 # 0 6 * 6 6 S 3 9N yy 33N3CII3N03 7.S6 ) S 6 X - Z S = O S 3 3 #301 0001 NOIiyyN33N03 00T OT aaaaaaaaaaaaaaaaaaa^aaaaaaaaa'aaaaaaaaa^aaaaaaaaaaaaaaaaaaat/ ot* 1 r OS 3 3 * A > 1 rGr . ' # V". - > * z z z z z z z z z z z z z z z z z z z z z z JTJTJTJTZ-JT<;* i ' - 9 8 y n w i. y Lr- C; S S S - 1 = 3 d0 1 S aaaaaaaaQaaaaaoaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaz 01 Q# i.2 * 0 = U1 1 0 3 MK'Oia S /S = 0 1 / 1 1 lO dlN G #9 3 9 3 8 '1 #010#/*# # 3 1 I<s T ! Z # I L V Z '2 Z # Z rS 9'0 if P 1 i_L " 0 # G # 9 S S `0 #S S I T 0 0000*0001 0000 *000 T 0 0 0 0 *OOS 0 0 0 0 *OOS o o o o *o s s 0000o s s 0 0 0 0 'S S T 0 0 0 0 'SSX S /28 S /18 6 /18 8 /SI 81 /#8 9 T /OB #S /8 SS /#8 8 L 9 s i? G 1 0WW09 "j/Dm :s^ T U f| o o i e j x u 3 u o j 3 : J O p F J U D fV lT Q T3 e N ^ u s i i ' i s n f p y d t 4 ''5 Q "3N03 1I/0 y38WfiN T/6 m 000 s e ^ n u n u 0 sauixjL 1 5 5 1 : UOT4PJ4UJ U03 IPT^TUI 3 1soa :e jn p s^ jj SS 3iy0103 3 0 3 1 3 3 3 0 S I\'O I O y iN 3 3 N 0 3 1 S 3 1 a m O H MG113A # 9 1 1 0 1 OS T- 3 3 " 3WI1 1S31 : 3iOQ 1 S 3I : uoT}aT.jDS3G atomes OO r T - f r O S f : 3W0N 3 1 1 3 x30d33 OlUa X01331W 01 * ^ ^A STATISTICAL REPORT FOR DATA FILE J2904-1.F E S T IM A T IN G EQ U A T IO N : LOG C = 0 . 6 4 6 6 ;< LOG b + 2 . 5 1 5 0 NUMBER OF DATA P A IR S USED: 8 RESIDUAL VARIANCE: 0 .0 0 2 0 1 95'/. CONFIDENCE FACTOR: 1 . 0 9 4 0 2 FOR ECSO CORRELATION COEFFIC IENT: R = 0 .9 9 3 3 1 EFFEC TIV E CONCENTRATIONS (957. CONFIDENCE RANGE*: EC 5 0 = 3 2 7 . 3 1 9 2 m g /L EC 2 0 = 1 3 3 . 5 5 5 5 m g / L EC 10 = 7 9 . 0 5 5 1 m g /L EC 1 = 1 6 . 7 7 0 1 m a /L ( 2 9 9 . 1 9 0 4 TO 3 5 8 . 0 9 2 6 ) ( 1 1 5 . 6 2 6 7 TO 1 5 4 .2 6 4 3 .( 6 5 . 0 0 9 3 TO 9 6 . 1 3 4 9 ) * * * ( 1 1 . 6 3 9 6 TO 2 4 . 1 6 2 0 ) * * * * * * EXTRAPOLATED RESULTS