Document 44r2ZJjayjrJ4MK9gRybVJEpR

FOR DU PONT USE ONLY AR226-2890 Copies to: E. I. du Pont de Nemours and Company Haskell La'.oratory for Toxicology and Industrial Medicine Elkton Road, Ne -ark, Delaware 19711 HASKELL LABORATORY REPORT NO. 866-80 Material Tested Haskell No. 13,384 . Other Codes/Synonyms Study Initiated/Completed 7/17/80 - 8/19/80 rial aitted by C&P Dept. J a c .3on Lab INHALATION MEDIAN LETHAL CONCENTRATION (LC50) he purpose of this investigation was to determine the 4-hour LC50 iin male albino Crl:CD rats. Procedures: A. general: Groups of 10 male, albino Crl:CD rats, approximately 8 weeks old and weighing :rom 224 to 293 g, were exposed via inhalation to atmospheres o f M I H i n alr for sin8le -4-hr periods. Except during exposure, rats suspended stainless-- steel re mesh cages. Purina Certified Rodent Chow 05002 and water were suppt ieX * d libitum. During exposure all rats were observed, clinical signs not4 '.'cf ihamber temperature monitored. Following exposur , rats were weighed and observed daily (excluding weekends) for a 14-day recovery period. Generation: The sample was liquified by heating to approximately 100*0. A 50 cc plastic syringe wrapped in heating tape was loaded with liquified sample and placed on syringe drive. The sample was syringe driven through a Spraying Systems Nebulizer. The aerosolized stream sprayed into a heated, 3 neck, 1 CesparryShnRTzed. Does not contain TSCA CBI round bottom flask. Dilution air was added and the resultant vapor/aerosoi mixture went through heated glass connectors into the top of a 20 1 glass I chamber containing the test r. . Analytical: Chamber samples were collected approximately every 1/2 hr by drawing a known volume of chamber atmosphere through 2 tandem midget implngers. The impingers contained acetone (ACS grade) as the trapping solvent. Samples were analyzed using a Hewlett Packard 5730A gas chromato graph equipped with a flame ionization detector. The column was a 6' x 1/4" O.D. glass coil packed with 202 Neopentyl and Glycol Sebacate on 60/80 mesh chromosorb PAH. Oven temperature wcs programmed tc hold at 80C for 2 minutes then increase 8C a minute to 220C and hold at that temperature for 4 minutes. __________ [concentrations were based on the second lars out in the G.C. chromatograph. This peak is identified asl on the basis of the G. C. peak height area and the reportesc<r sample composition. Chamber atmosoher^concgntrations were ,,ien calculated and expressed as the e q u l v a l e n t f f l H m | R c o n c e n c r a t i o n (mg/1). Results: Observations: During all exposures rats exhibited reduced response to sound, Irregular respiration, salivation, vet fur, and tearing from eyes. In exposures 1 8 . 2 mg/1 rats exhibited occasional gasping and varying degrees cf dark red ocular and nasal discharge. In correlation to dosage, rats exhibited slight to severe weight loss, lasting 24-96 hrs post-exposure followed by normal weight gain. All deaths occurred 24-96 hrs post-exposure. Mortality Data: Me3RW B i H i Concentration mg/1 (range) Standard Deviation mg/1 Fractional Mortality (# deaths/# exposed) 3.5 (2.1-4.4) 13.3 (9.2-15.9) 18.2 (12.1-23.2) 35.7 (27.3-51.4) + 0.8 + 2.0 + 4.0 + 7.5 0/10 1/10 2/10 5/10 The LC5G** was calculated to be 35.3 mg/1 with a lower limit of 23.8 mg/1 and an upper limit of 790.4 mg/1 with 952 confidence limits. 2not contain T S C A C B I Company Sanitized. Does -J m m.Summary: ,, Groups of 8 week old Crl:CD male rats were exposed to vapors of Ifor single 4 hr periods. The LC50** was calculated to be 35.3 mg/1 "% * contiidence limits and a range of 23.8 - 790.4 mg/1). Clinical signs observed during exposure included reduced response to sound, irregular respiration, salivation, wet fur, and tearing from eyes- A dark red discharge was exhibited from eyes and nose of rats exposed to IS. mg/1. Slight to severe weight loss was observed 2 4 - % hrs P^c - e x p o s u r e in accordance8with dosage and followed by normal weight gain. All deaths occurred 2 4 - % hrs post-exposure. This exposure was the highest concentration obtainable using the epreviously mentioned generation procedures and conditions. Report by: 'oseph C. Marnili leport No Approved :y : Gerald L. Kennedy Chief. Acute Investigations Section -3