Document 44og3jdVmYEdDk43rw7QR5kpx

ACUTE TOXICITY TO FISH (MUMMICHOG) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 1. The testsample isFC203. Currentinformationindicatesitisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol,2.66 % Sultone foamer, 3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: DTNSRDC Standard StaticMarine Bioassay Procedure for Shipboard Chemicals (Liberatore). Type: Acute static GLP: No Year completed: 1979 Species: Fundulusheteroclitus Supplier: Commercial supplierinMassachusetts. Analyticalmonitoring: Temperature,pH, salinitayn,d DO Exposure period: 96-hours Statisticamlethods: LC50 valuescalculateudsingthe Stephan computer program, 1978. Test fishage: Juveniles Length and weight: Average length 43 mm Average weight 0.91g (wet) Loading: Not given. Pretreatment:None Test conditions: Dilutionwater: InstanOtcean@ dissolvinigndeionizedwater Dilutionwater chemistry: pH 8.2 Salinity 15 ppt Alkalinity: 52 mg/L as CaC03 Conductivity: 23,000pmhos/cm Lighting:A dailyphotoperiodof16 hourslighatnd 8 hoursdarkwas maintainedthroughoutthe testingperiod. Stock and testsolutionpreparation: The testsolutionwas added directly to the dilutiownater and mixed by stirrinwgitha glass rod. Concentratiodnossingrate:Once StabiliotfythetestchemicaslolutionsN:otnoted Exposurevessels:19.6Lglasjsarcsontaini1n5gL oftesstolution. Number ofreplicate2s: Number offishperreplicat5e: Numberofconcentrationfsi:vpelusa blankcontrol Waterchemistrdyuringthestudy: Salinirtaynge:(0-9h6ours) 15- 17ppt(contreoxlposure) 16 - 17 ppt(3600 mg/L exposure)* pH range (0-96hours): 7.2- 8.1(controelxposure) 6.7- 8.1(3600mg/L exposure) Temperature range (0-96hours): 19.5*C Dissolvedoxygen range (0-96hours): 6.2- 8.3mg/L (controelxposure) 3.0- 8.2mg/L (3600mg/L exposure) RESULTS Nominal concentrations: Bk control6,00,1000,1700, 2400, and 3600 mg/L Element values: 96-hour LC50 = 2500 (1700 - 3600) mg/L 96-hourNOEC = <600 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conductedon themixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsample 96-hourLC50formummichog was determinedto be 2500 mg/L witha 95% confidenceintervaolf 1700 to3600 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking= 2. Testingmeets the criterfioarqualittyesting. However, the sample puritywas notpropedy characterizeadnd the study lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution. REFERENCES Testwas conducted by EG&G Bionomics,ofWareham, MA atthe requestof the3M Company, St.Paul,MN, Lab Request number 4971 S, 1979. OTHER Last changed: 6/27/00 1 ACUTE TOXICITY OF 7902 TO THE COMMON MMMICHOG (Fundulus'heteroclitus). LR Al EAZ 7 :).41.s TOXICITY TEST REPORT SUBMITTED TO THE 3M COMPANY ST. PAUL, MINNESOTA REPORT #BW-79-8-526 E G & G, Bionomics Aquatic Toxicology Laboratory 790 Main Street Wareham, Massachusetts August, 1979 INTRODUCTION The purpose of this study was to estimate the acute toxicity of 7902 to the common mummichog (Fundulus heteroclitus) under static conditions. A 96-hour definitive test was conducted from 27-31 July 19.79 at the Aquatic Toxicology Laboratory of E G &.-G, .Bionomics, Wareham, Massachusetts. All raw data generated are stored at the a:bove location. MATERIALS AND METHODS.- Unless otherwise stated, procedures used in this acute toxicity test followed those described in "DTNSRDC Standard Static Marine Bioassay Procedure for Shipboard Chemicals" (Liberatore). All values are reported to various levels of significance depending on the accuracy of the measuring devices employed during any one process. The 7902.- an amber liquid, received from the 3M Company, St. Paul, Minnesota on 6 July 1979, was tested on a formulation basis., Nominal test concentrations are reported as milligram .0f.7902 per liter of test solution (mg/1). The-common mummichog (lot #79A7) used in this study-were obtained-from a commercial fish supplier in Massachusetts.. Upon receipt at Bionomics, the fish were held in a 600-1 fiberglass 2 tank containin9 a solution of Instant OceanR which was recirculated through dolomite in a biologically active filter bed to preserve water quality during holding and acclimation. This water was characterized as having a pH ra.nge of -0-'7@- a dissolved oxygen (DO) range of 89-90%'of saturation (Weekly Record of Fish Holding Water Characteristics, Vol. I),.a specific conductance range of 18,000-23,000 micromhos per centimeter (_pmhos/cm),a salinity of 15-19 parts per thousand (p/oo) and a temperature range of 19.0-20.50C (.Da.ilRye-cord of Fish Holding Conditions, July 1979). Experimental animals were maintained under these* conditions for a minimum of 14 days. All fish were fed a dry pelleted food,rad libitum, daily except during the 48-hours prior to testing. There was no mortality durincj.this2 day period. The pH was xqeasured with a Model 1175 Instrumentation Laboratory PH meter and combination electrode, the temperature with a-"@ial thermometer, the DQ with. a Model 157 YSI meter and probe, the specific conductance with a 14odel*133. YSI conductivity meter, and-the salinity with an Ai4eri@can()ptical refrictometer. The definitive teiit'wasconducted in 19.6-t glass jars which contained 15 1 of test solution. The dilution water used in this test was -prepared by adding Instant OceanR to deionized water and mixing until -dissolved. This water had a pH o<@F) a total alkalinity as calcium carbonate of 52 mg/i (APRA et al., 1975), a specific conductivity of 23,000 pmhos/cm and a salinity 3 of 15 o/oo (ReconstitutedWater, Water Quality Analysis, Vol. II). The 7902 was added directly to the dilution water and mixed by stirring with a glass rod. Control jars (A and B replicates)-containing the.same dilution. water and maintained under the same conditions as-test cqncen- . trations, but containing no 7902, were e stablished. All test solution temperatures were controlled by a system designed to. maintain temperatures at 19'-+10C. All test-solutions were gently aerated during the exposure period. The photoperiod during acclimation and testing was 16 hours light and 8 hours darkness. Ten common mummichog (five per replicate) with a mean (.range, N=30) wet weight and total length of 0.91 (0.28-2.79).grami'-@and 43 (32-60) millimeters (Fish Weights and Lengths Log, Vol. III) were.,randomly distributed to each test concentration within 15 minutes after the solutions we're pxepared. Mortalities were recorded and removed from each test jar every .24 hours during testing. Biological observations of the fish and obs6rvations*of the physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pHi DO concentration and salinity of test solutions were measured at 0, 24, 48, 72 and 96 hours in both replicates of the control, high, middle and low test concentrations. Temperature 4 was also measured in each control jar replicate at the above mentioned time intervals. When results permit, the concentrations tested and the corres-,-ponding mortality data deri'ved-from-"--the-toxicity,teasrte used-@to estimate 24-,. 48-,-72- and 96-hour median lethal.concentr-ations (LC50) and 95%-confidence intervals. The LC50 is defined as the concentration (nominal or measured) of the test compound in dilution water which caused 50% mortality in*th"etest animal population at the stated exposure interval. The computer program utilized (Stephan, 1978, personal co=unication) estimates LCSO values using one of three statistical methods in the following order of preference: moving average angle analysis, probit analysis, binomial probability. The'. method selected is determined by the characteristics of the data bcise (.-i.e.p.resence or absence of 100% mortality, number of partial mortalities, etc.l. The computer program scans the data'"base, identifies the most preferred statistical method and performs the analysis. RF.SULTS, The 96-hour LC50 fox the common mummichog exposed to 7902, esti- . mated by the binomial probabil4ity method, was@ Table 1 5 summarizes the 24-,, 48-i 72- and 96-hour LC50's and 95% confidence intervals and states the no discernible'' ef f'ect'-Concentration through 96-'hours. The no effect concentration is-the highest concentration' tested at which there were no mortalities or observed behavioral and'physical ab*n''o'r'm"alities, loss of equilibrium, at surface, 'darkened picjm'entation. The pH, DO and salinity measured during the test are presented in Table 2. The temperature of the control sol-ution was 0 19.5 c during exposure. Table 3 presents the nominal test concentrations, the corresponding cumulative mortalities and the observations made. .......... ....... Deviations from th6Protocol Cited -'Th,6 common mummichog were o-4.3 centimeters long. Nineteen. point six* liter widemouth glass bottles were used as test containers. Instant Ocean R solution was used for acclimation and testing." The fish*were acclimated in recirculating water. The* salinity for acclimation and testing was 15 o/oo. Fish were acclimated in water with a pH range of 7.0-7.2 and tested in water having a pH of 8.2. 6 The test jars contained 15 L-of solution and 5 test fish. 77 The'test conce'ntritionsweria.ri6@lic'aied.''. LC50's'were calculated by StephAn- 1978, personal co-mmunication. -7 It -isour judgment'..thatnone of these Aeviations.have affected: the.integrity of the test results... LITERATURE CITED APRA, AWWA,, WPCF. of water.and 1975. Standard methods for the examination wastewater. .-14t]@Edition-, Washington,,. D.C. 1193 pp. Liberatore,,@Giacomo'." David W. Taylor Navel Ship Researcli:'and Development Center. Standard Static Marine Bioassay.: Procedure for Shipboard Chemicals. Report TM-28-76-29. Stephan, Charles.. 1978. U.S. EPA, Environmental'Research Laboratory, Duluth, Minnesota. Personal communication. SUBMITTED BY: PREJ@IIMD BY:-REVIEWED BY: APPROVED BY: DATA AUDITED BY: E G G, Bionomics Aquatic Toxicology Laboratory 790 Main Street Wareham, Massachusetts -.August, 1979Charlotte' Stiefdl Principal Inv6stigator Robiert J. Buccafusco Study Directo'ra George A. Cary Director, Aquat4c Biology Ronni lr"xasny Director, Qual3*.tyAssdrance Unit Table . The, 24-t-,48-,@72- and 96-hour LC50.,values and..95% confidence interv the common mummichdg (Fundulus hete'rocl-itus)exposed to 7902. 24 hour.a .............. .......... 0 (Imgzt.) .. ..... No dis LC5 effect co 48 hour b .72.hour 96.hour b. through 96 ... .......... . .......................... >3600 2900 2900 2500 <6 (2400-3600)c (.2400-3600) (1700-3600) a Empirally estimated. Estimated by the binomial probability method. c 95% confidence intervals. d Lowest concentration tested. Table 2 The PH",i.DO.,and ma@l@nity Measux.eigents made during the 96-hour toxicity;teat with:79 mummichp4 . (Pundulus heteroclitus). ]Ranges of measurements made in rep licate conce repor:ted Now inal concentration (mg/t) 0 hour 24 ho'ur- 48.hour 72 -hour:, * pH 3i 600 8.1 8.0-8.1 6.7 6.7 1,700 8.0 8.0 6.8 6.7-6.a 600 control B..o 8.0-8.1 7.9-8.1 7.9-8.0 7.0-7..l 7.2-7.5 7,..1-7.'2 7'.2-7 .'6 DO (mg/1) 3,600 1,700 600 control 8.2 a 748-7.9 (84-850) 8.0-8.&(86-88) 7.7-7.9(83-851 8.2(88) 7.7(83) 8.0-8.3(86o-.-89) 7.4-7.5(8d-81) 3.X 3,j7(33-40) 4.5-5@'2 (48@56)'. 6.0-7.3(65-78) 7.1-8.1(76-87) 3 46'1!r3.8*(j3-4.1 5.3-6.9(57-74) 6.2-8.1(67-87) salinity (0/00) 3,600 1,700 600 control 16 16 17 15-16 15-16 16' 15 is 16 15 15 16 17: 17 16-17 17rl7 a t of saturation at 190C. .Table 3 Concentrations tested and corresponding cumulative mortalities of the common mummichog (Fundulus. bet6r6clitus) exposed t6'*7'902-for 24-- 48, 7 2 -.-iii9d6 '7 hours. Ten fish were exposed to each concen:Eiiition,, five fish per replicate-.-.--..,- .. . . . . . . . . . . . . . .. .. . . . . . . . . . a.. Nominal concentration Cumulative morta:l:iti-es('A 'and B replicates)' (mg/t) .. ....2.4..ho.u.r........48..h.o.ur..... 72 . hour 96 hour 3,600 .21400 11700 1,00 0 600 'control 0 b,c,d,e 10 10 b,d 0 1b,d 1b 0b 0b 0 b 0 0 0 0 0b 0b 0 0 0 10 2 b,d 0 b,,d b 0 b 0 .0 a Foam present on surface of all solutions, except .throughout testing. b Some fish were at the surface. c some fish had partially lost their equilibrium. d Some fish were lethargic. e .": . -..I ... . - I . I.. - Some fish were respiring rapidly. the controls,