Document 44mdDb1RJDJYx333m3xRamV6V
AR 226 - 0767
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
IESTSUBSTANCE
0000
Identity: rMeifxetrurreedctoonatasinPiFnOgSp,erFfCl-u9o5ro,oocrtaansesaulcfoomnpatoen,ewnhtiocfhFmCa-y20a3lsCoF-bXe or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: FC-203CF.
CTurhreen3tMinpfroordmuacttiioonnilnodtincuatmebseirtiws aasmi3x0t0u3r4e.ofTh1e%tPesFtOsSa,mp2l0e%is
diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3%
sodium octyl sulfate, 1% triethanolamine, 2% sufonated alkyl ether saits, and
0.05% tolyltriazole.
The following summary applies to a mixture with incompletely rcehfalreaccttteorxiizcietdycoofnctehnetfrlautoirooncshoefmiicmaplurciotimepso. nDeanttaofmtahye ntoetstacscaumrpaltee.ly
METHOD:
000000
MMaectrhooidnv:erStteabnrdaatersd,GauniddeAmfoprhiCbonidauncst(inAgSTAcMuEt7e2T9o-x8i8cait)y,T1e9s9t5s with Fishes,
Type: Acute static renewal
GLP: No
YSepeacriceosm:plPeitmeedp:hal1e9s99promelas
Supplier: In-house culture. Inc., Fort Collins, CO.
Original
brood
stock from Aquatic Biosystems,
Analytical monitoring: Temperature, pH, conductivity, and dissolved
oxygen.
Exposure period: 96-hours
Statistical methods: The LCs values were calculated by Trimmed
Spearman-Karber method. The NOEC values were calculated by Fisher's
Exact test using Toxstat statistical software, Version 3.5.
Test fish age: Juveniles, 26 to 28 days old
-
Length and weight: Average length = 17 mm
Loading: 0.12gL Average weight = 0.037 g (wet)
Pretreatment: None
Test conditions:
Dilution water: Dechlorinated City of Duluth, MN tap water, post-
Dcialrubtoinonfilwtaert.eTrhcehweamtiesrtrwya:s aerated for 24 hours prior to the test. _
AHlakradlinneistsy:: 4524 mmgg//LL aass CCaaCCOO;s
pH:
803
001300
Lighting: Cool-white fluorescent and 8 hours dark was maintained
ltihghrtos.ughAoudatitlyhephteosttoipnegrpieordioodf.16
hours
light
Stotothcekdailnudtitoenswtastoelruotnioan vporleupamrea/tvioolnu:meTbhaesitse.st material was added directly
Concentrations dosing rate: Renewal at 48 hours.
SEtxapboilsiutryeofvetshseetless:t
chemical solutions: 4 L wide mouth glass
Not jars
noted. containing
3
L
test
solutions.
Number of replicates: 2
NNuummbbeerroofffciosnhcepnetrraretpiloincsa:te:fiv1e0.plus a blank control
`Water chemistry during the study:
pH range (0-96 hours):
7.80 --8.03 (control exposure)
7.33 7.89 (720 mg/L exposure)"
TCoenmdpuecrtaitvuirtey rraannggee ((00--9966 hhoouurrss)):: 24.8 -25.6 C
114235---113529
pmhos/cm pmhos/om
(control exposure) (720 mg/L exposure)*
Dissolved oxygen range (0-96 hours):
7.4-83 mglL (control exposure)
*
720
5.28.4 mg (720 mg/L exposure)" mg. (intermediate concentration) exposure values
used
because the highest two concentrations resulted in total mortality by 24
hours.
Reswts
000000
Nominal concentrations: Blank control, 259, 432, 720, 1200, and 2000
mg/L.
Element values: 96-hour LCso = 839 (766 -- 920) mg/L 96-hour NOEC = 720 mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on the mixture as described in the Test SthuebsftlauonrcoechReemmicaarlksprfoipeoldr.tioTnhealvoanleu.es reported apply to that mixture and not
c0 oncl0 usio0 ns 00000000
`The test sample 96-hour LCso for fathead minnow was determined to be 839 _
mOgb/sLerwvietdh aEf9fe5c%t Ccoonncfeindternacteioinnt(erNvOalEoCf)7i6s672to09m2g0lLm.g/L. The 96-hour No
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
001301
p0 ata0 uau0 ry 0000000000
taRenesaltliiyantbgii.clailtHyoc:woneKfviieirrmm,iastsciahomnropaflnektihpneugrai=tmy2o.wuanTsthoenfostftipuurdooyrpoemcrehleeytmcishcatarhlaecpctrreoiprtoeirrziteaidofonarniqdnutaithlieltaycks solution.
R0 EFERENCES0000
This test was conducted by AScl Corporation, Duluth Environmental Testing
Division, Duluth, MN at the requestof the 3M Company, St. Paul, MN, Lab
Request number W1127, 1999.
omer
0000
Last changed: 6/28/00
001302
oF .
`EnviroamentalASTeelsiCnogrpDoirvaitsiioonn ASeSlubSmuidceydI0DF35M0-10.209698
STUDYTITLE
ACUTE TOXICITY OF FC-203CF TO FATHEAD MINNOW (Pimephales promelas)
. DATASTANDARD
ASTME 729 - 88a
STUDY COMPLETED
February 22, 1999
TESTINGFACILITY
ASI Corporation Environmental Testing Division . 4444 Airpark Boulevard
Duluth, MN 55811
Tel. No. (218) 722-4040
:
Fax No. (218) 722.2592
STUDYIDENTIFICATIONNUMBERS
-
ASI Study IDF 5010-068
3M Company Lab Request W1127
S`Sppoonnssoorr:S3MuIDYdW11y27 (FC-205CF)
1
\
001303
.
EnviroameotalATsecstiCnogmDoivriisiaonn
SSlubSmiytedDr33M01-020698
1.0 GENERAL INFORMATION
Study Tide
`Sponsor's Representative
-
Sponsor's Lab Requests
{| Testing Facility
1 i
|EPrinciT pal Investigator Ting Fi Bi
Definitive Test Dates Data Validation and Report Review
Retention of Raw Data and Final Report I Location of Raw Data and Final Report I|
"Acute Toxicity of FC-203CF to Fathead |
Minnow (Pimephales promelas).
i
ASTM Designation: E 729-88.
|
3M Environmental Technology and Safety
Services, Building 2-3E-09, 935 Bush Avenue,
St. Paul, MN 55133; Tel No. (651) 778-7452.
Susan Beach, 3M Environmental Technology | and Safety Services, Building 2-3E-09, 935
Bush Avenue, St. Paul, MN 55133;
Tel No. (651) 778-7452.
W1127.
ASI Corporation
Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218)
722-4040.
[oepienss1]
TY SS { [oomeiom| February 9-13, 1999.
Alan Mozol
Report Signature: |
Z 7 2 |! `Two years from the date study is completed.
`AScl Corporation Testing Division, 4444 ANior.pa(r2k18B)ou7l2e2v-a4r0d4,0.Duluth, MN 55811; Tel. |
SSppoonnssoorr:S3yM ID# W112 (FC203CE)
2
001304
EnvinmSeunbamiltATSecdslitonCgo3r4Dpi-ovs2iii5oo9nn Asc Su Io Soioaes
2.0 OBJECTIVES)
"nToo odbesteerrvmaibnleetehfefe9c6t-choonucren1tr0a%tiaonnd(5N0O%ECl)etohafltchoencteesnttrsautbisotnasnc(e,LCFgC-a2n0d3CLFC, ,forafnadthtehaed9m6i-nhonuorw |
(Pimephales promelas) under static-renewal test conditions.
"This study was conducted according to ASTM Designation: E 729-88a, Standard Guide for
Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians, 1995.
3.0 TEST METHODS
"Test Substance
l{
Test Organisms
|| |
{|
| Dilution Water
I
P1r)o,psetratbilees,:anFdC-m2is0c3iCbFle, waimtbhewratseorl.ution, specific gravity = 1.0 (water =
2Te0s0t0comngc/eLn.traTteisontss:olu0ti(odnilpurtieopnarweadtebrycvoontlruoml)e 2a5d9d,iti4o3n2,of72t0es,t s1u2b0s0t,anacned to dilution water followed by stirring. | oSbotuaricnee:d fHeraolmthAyS,cjluvienn-ihloeusfeatchuelatdurmesi.nnOorwig(inPailmebprhoaoldesstporcokmefloarst)hewecruleture | was obiained from Aquatic Biosystems. Inc., Fort Collins, Colorado,
Hhoaltdcihnegd/oacnclJiamnautairoyn:12,Th1e3,baantdch1o4f, f1i9s9h9a.ccTlihmeatfeidshfowrertehisacsctluidmyatweadsto test wcoansdi0t%io,nsanfdort1h4e fdiasyhs.werFeornotthefe1d4 fdoary2s6prheocuerdsinbgeftoerset itensittiiantiitoina,timoonr.taAligtey of the organisms at test initiation was 26 to 28 days. The fish mean length wwaass c1a.l7cu+lat0e.d2 tcomb,ea0n.d12megaonfwfeitshwpeeirghLt tweastss3ol7ut+ion1.2 mg. Loading rate |
Use in test: Used 10 organisms/replicate and 2 replicates/test
-
concentration.
| Source: Dilution water was carbon treated City of Duluth, MN, tap water.
`The most recent chemical analysis is in Raw Data Package. The same
I
batch of dilution water was used for the entire acclimation period and the |
toxicity test. The batch of dilution water was aerated continuously during |
storage.
|
Characteristics: At test time, the dilution water had a total hardness 42
mg/L, an alkalinity of 54 mg/L (both as CaCO;) and a pH of 8.03.
SSpohnsoorn: Suadmy IDA W127 (FC205CR)
3
001305
Environ!ATetsiCngomborroann ASlunSmittyed1a044$012055
[ECxhpaomsbuerres
[ espason { Observations
{I Endpoint Calculations L
Type: 4-L wide mouth clear glass jar.
tUesste, ikneptetstc:ove3rLed,ofedxicleupttiownhweanteerxpoerritemsetntsaolluoibosne/rcvhaatmiboensr.orDsuorluitnigonthe | irnetneerwvaall,s awnedreaemraatdieo.n wTaesstnsootlustuipopnlsiewderduerirnegnetwheedexaptotshuere4.8-hour test || `Duration: 96 hours.
"fDlaviolryepscheonttoplearmiposd.: 16 light and 8h dark, maintained using cool-white
Temperature: 25.0 + 1C.
}
Bsitroelsosg,icaanld: deDaaidlyte-stToersgtanoirsgmasn.isms with abnormal behavior and signs of {
aWnadtearlicahleimniitsyt,ry(:2) (d1a)ilAy-t tteesmtpeirniattiuartei,onDaOn,d scoolnudtuicotnivrietnye,waaln-d tpoHta.l hAalrldness
determinations were made according to APHA methods (1998).
LC; US EPA ICp Analysis.
LCs: Spearman-Karber Method.
|NOEC: Fisher's Exact Test(Toxsiat Version 3.5)
_
3.0 RESULTS
{ TCeosntfiSduebnscteanIcneteTrovaxlisc)i.tyPEenrdtipnoeinnttsdaatnadar(e95in% Table 1. || {
{
|| 2m4g-/hL)L.Cs: 906 mg/L (95% C1 862.952
4mg8/hL)L.Cs: 839 mg/L (95% C1766-920 .
7mg2/hL)L.Cs: 839 mg/L 95% CI 766-920
96-h Ly: mg/L).
839 mg/L (95% CI 766-920
96h LC;:. 576 mg/L (95% CI Not Reliable).
96h NOEC: 720 mg/L.
SSproanmsoorr;SIuMb IDA WHIZ? (FC203CP)
`
001306
EnvironmenalATsienCgorpDoirvastiioonn AsScuSbudmyiIttDoFe3MSd0-10209698
Ranges of Water Chemistry Parameters
L
_
Hardness (mg/L as CaCO): 42-44.
Alkalinity (as CaCO mg/L): 46-52.
Temperature (C): 24.825.6.
DO (mg/L): 5.28.4. renewal at the 48-hour
Maintained via test interval.
solution
PH: 7338.03.
{
|Specific conductivity qumhostem): 125-177. |
4.0 TEST QA/QC
Qc Ciera
low
7]
.
| tMhoarntal1i0t%y.in control exposure must not be more| 0% mortality.
`The lowest DO concentration at test | tsaetrumriantaitoinonvamluusetatbeteastt tleeamspter6a0t%ureo.f the air
`The lowest DO concentration measured was
63% of the air saturation value at 25C.
| Test duration must be 96 h.
"The test duration was 96 h.
| Reference Substance Toxicity Test.
RtheefeRraenwceDattoxaicPiatcykategset.ing data is included in
5.0 REFERENCES
[American Public Health Association (PHA). 1998. Standard Methods for the Examination of |
| `Water and Wastewater. APHA, Washington, D.C.
|
|{CAoSnTduMc.ti1n9g95A.cuSteectTiooxnic1i1t.yVToelsutsmewi1t1h.0F5i.sheDse,siMgancartoiionnv:erEte7b2r9a-t8e8sa,.aSntdanAdmaprhdibGiuaindse. for
SSppoonnssoorr:St3udMy IDA W1127 (FC-203CF)
5
001307
BvioA rmSense!e JoarnegnmpBiosoon--
TABLE 1. ACUTE TOXICITY OF FC-203CF TO FATHEAD MINNOW (P. promelas)
Co [combinumberor adOpieodPoeMorais
Concentration(mg/L)
ue
Jeb me
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[ [ @ |vso0 | | s| 0 o | | oeoo | | oeoo ||
[0m | vo | seo [| 4a | sem |
[ow | wa | maw | oem | wom |
+ Peonasianpgureenthssis
LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS
[ [Ceoeeeo,rT Te w erT| aovm corte
|] |
| enc |eTe |
[ mwiee Tee Tew
[ooor ie 1 T |w o owe mae ||
Valuesbed on soestoi ceril ons.
.
-- soSY
.
001308