Document 3w8gvRj5rmqpXbJXkMeqvqdx

A M & 'W lf BIODEGRADATION TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-Xor R1904. (Glycine, Nethyl-N-[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C7 fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg. date 3/97. The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data does not accurately relate biodegradation of the test sample with that of the test substance. METHOD: Method: Not noted. Type: Aerobic GLP: No Year completed: 1997 Contact time: 28 days Inoculum: Polyseed NX, lot D257 RESULTS COD: 462,000 mg 0 2/ kg chemical BOD First Replicate BOD Day Mean Value (mg/kg) 5 <39,800 10 186,900 20 82,700 28 289,000 Second Replicate BOD Mean Value (mg/kg) <39,800 156,750 179,400 289,000 Remarks: The BOD and COD values are expected to be the result of biodegradation of the organic solvent components of the test sample. These values are not expected to be representative of the Perfluorooctanesulfonylamido(ethyl)acetate. 000353 CONCLUSIONS This testing indicated that some portion of the test sample biodegraded in aerobic environments capable of supporting biodegradation. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. This study lacks information on the methodology. Testing cannot conclude what portions of the test sample were responsible for the apparent biodegradation values. Comparison with the COD value for determination of the level of biodegradation is not valuable without a description of how the COD value was obtained. REFERENCES This study was conducted at AScI Corporation of Duluth, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00 0Q354 SAuSEbAcmnISv-icDtitrIeoEdCnTmotDorepn3SotMrtaualdtiTCyoeonIsm/DtAipnS#agcn5ID0y-1Di-v0ui-7sl0u/i69oth87n R1904 BOD REPORT FOR 3M COMPANY Submitted to 3M Environmental Technology and Safety Services 9 3 5 Bush Avenue Building 2-3E-09 PO Box 33331 St. Paul, MN 55133-3331 Prepared by AScI Corporation/AScl-Duluth Environmental Testing Division 4 4 4 4 Airpark Boulevard Duluth, Minnesota 55811 SSppoonnssoorr:St3uMdyCIDom# pRa1n9y04 JULY 1997 000355 COD = 462,000 mg 0 2 / kg chemical. Dates of BOD test: 6/11/97 - 7/9/97. Water bath temperature range during the test: 19.0 - 20.0C. Lot number of Polyseed NX: D257. SAuSEbAcmnISv-icDitrtIeoEdCnTmotDorepn3SotMrtaualtdiTCyoeonIsm/DtAipnS#agcn5IDy0-D1i-v0ui-7ls0u/i69oth87n TABLE 1. BOD Dilution Information for R 1904 Sample ID (mg/L) 10 18 25 50 Stock Solution Concentration (mg/L) 1005.04 1005.04 1005.04 1005.04 Sample Volume (ml) 3.0 5.5 7.5 15.0 Sample Concentration (mg/L) 10.1 18.4 25.1 50.3 TABLE 2. BOD Blank Calculations for R 1904 Sample ID Day FIRST REPLICATE SECOND REPLICATE Initial D.O. Final D.O. D.O. Drop Initial D.O. Final D.O. D.O. Drop Blank 5 8.89 8.90 -0.01 8.84 8.84 0 Blank 10 8.89 8.78 0.11 8.84 8.64 0.20 Blank 20 8.89 8.52 0.37 8.89 8.50 0.34 Blank 28 8.89 8.40 0.49 8.84 8.52 0.32 SSppoonnssoorr:St3uMdyCIDom# pRa1n9y04 -i 000356 SAuSEbAcmnISv-icDtitrIeoEdCnTmotDorepn3SotMrtaualtdiCTyoeonIsm/DtAipnS#agcn5IyD0-D1i-v0ui-7ls0u/i96oth78n TABLE 3. BOD Seed Correction Factor Calculations for R 1904 Smeeld/BCoottnlee. Day IDni.tOia.l FIRST REPLICATE DFi.nOa.l DD.rOop. SCF* 10 5 8.90 6.01 2.89 0.58 10 10 8.90 5.87 3.03 0.61 10 20 8.90 4.46 4.44 0.89 10 28 8.90 3.52 5.38 1.08 SECOND REPLICATE IDni.tOia.l DFi.nOa.l DD.rOop. 8.91 5.63 3.28 8.91 6.02 2.89 8.91 4.05 4.85 8.91 3.56 5.35 SCF 0.66 0.58 0.97 1.07 AvSeCrFage 0.62 0.60 0.93 1.08 a SCF = (D.O. drop * ml seed in test) / ml seed in seed blank. TABLE 4 . BOD Reference9 and Tox Blanks Calculations for R 1904 Cmogn/eL. Day SCF IDni.tOia.l FIRST REPLICATE DFi.nOa.l DD.rOop. CoDrrreocpted mBgO/LDb IDni.tOia.l SECOND REPLICATE DFi.nOa.l DDr.Oop. CoDrrreocpted mBOg/DL 0 5 0.62 8.90 4.80 4.72 4.10 205 8.89 4.64 4.25 3.63 182 50.3 5 0.62 8.91 2.34 6.57 5.95 298 8.90 3.62 5.28 4.66 233 a Reference = 150 mg glucose and 150 mg glutamic acid in 1 liter BOD dilution water, tested at 2% dilution. b BOD mg/L = (corrected D.O. drop) * 300 ml BOD volume / 6 ml reference. SSppoonnssoorr:St3uMdy CIDom# pRa1n9y04 - 2 - 000357 SAuSEbAcmnISv-icDitrtIeoEdCnTmotDorepn3SotMtraualdtiTCyoeonIsm/DtAipnS#agcn5IDy0-D1i-v0ui-7ls0u/i69oth87n TABLE 5. BOD Calculations for R 1904 Cmogn/eL. Day SCF FIRST REPLICATE IDni.tOia.l DFi.nOa.l DD.rOop. CoDrrdroepcte mBgO/kDg* SECOND REPLICATE IDni.tOia.l DFi.nOa.l DD.rOop. CoDrrreocpted mBgO/kDg 10.1 5 0.62 8.90 8.00 0.90 0.28 -- 8.91 8.32 0.59 -0.03 18.4 5 0.62 8.89 7.96 0.93 0.31 -- 8.90 7.83 1.07 0.45 25.1 5 0.62 8.90 7.74 1.16 0.54 - 8.90 7.72 1.18 0.56 50.3 5 0.62 8.88 6.61 2.27 1.65 32,800 8.90 6.70 2.20 1.58 -- _ -- 31,400 Mean <39,800 Mean <39,800 10.1 10 0.60 8.90 6.28 2.62 18.4 10 0.60 8.89 7.20 1.69 25.1 10 0.60 8.90 2.74 6.16 50.3 10 0.60 8.88 1.31 7.57 2.02 200,000 8.91 7.60 1.31 1.09 - 8.90 6.02 2.88 5.56 222,000 8.90 3.54 5.36 6.97 138,600 8.90 0.05 8.85 Mean 186,900 0.71 2.28 123,900 4.76 189,600 8.25 _ Mean 156,750 10.1 20 0.93 8.90 7.02 1.88 18.4 20 0.93 8.89 6.00 2.89 25.1 20 0.93 8.90 5.69 3.21 50.3 20 0.93 8.88 4.20 4.68 0.95 - 8.91 7.28 1.63 1.96 - 8.90 6.04 2.86 2.28 90,800 8.90 1.48 7.42 3.75 74,600 8.90 2.95 5.95 Mean 82,700 0.70 ~ 1.93 6.49 258,600 5.02 100,200 Mean 179,400 10.1 28 1.08 8.90 4.96 3.94 18.4 28 1.08 8.89 2.40 6.49 25.1 28 1.08 8.90 0.48 8.42 50.3 28 1.08 8.88 0 - 2.86 283,000 8.91 5.06 3.85 5.41 294,000 8.90 2.25 6.65 7.34 - 8.90 0.12 8.78 - - 8.90 0 - Mean 289,000 a BOD = corrected drop x 1,000,000 / test concentration. Omitted data points: D.O. drop corrected for SCF too low (< 2.0). Final D.O. < 0.50 mg/L. SSppoonnssoorr:St3uMdyCIDom# pRa1n9y04 - 3 - 000358 2.77 274,000 5.57 303,000 7.70 -- ---- Mean 289,000 SAuSEbAcnmlSv-icDitrtIeoEdCnTmotDorepn3oStMrataultdiTCoyenoIs/mDtAipnS#agcn5IDy0-D1i-v0ui-7ls0u/i96oth78n APPENDIX A - Raw Data Sheets SSppoonnssoorr: St3uMdy CIDom# pRa1n9y04 000359 BIODEGRADATION (METHYLENE BLUE ACTIVE SUBSTANCE) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, Nethyl-N-[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory as sample G1208-1 and replicate analysis as sample G1208-4. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C7fluoroalkyl carboxylate compounds. Test material was from 3M production lot 564. The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data does not accurately relate biodegradation of the test sample with that of the test substance. METHOD: Method: EPA Method 425.1 Type: Methylene Blue Active Substance GLP: No Year completed: 1989 RESULTS G1208-1: 2200 mg/kg G1208-4: 2800 mg/kg (duplicate of G1208-1) Duplicate EPA LAS Reference samples: 60,000 mg/kg 60,000 mg/kg CONCLUSIONS This testing indicated that the test sample exhibited minimal activity in this MBAS study. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY 000360 Reliability: Klimisch ranking 2. This study lacks information on the methodology and testing parameters. Test substance purity within the test sample was not sufficiently characterized. REFERENCES This study was conducted at Pace Analytical of Minneapolis, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00 00036 02/22/1989 ENVIRONMENTAL LABORATORY FINAL REPORT *** REVISED *** 1 LAB REQUEST NO. G1208 REQUESTOR NAME: EA REINER DEPARTMENT: 0535 PROJECT NO: 9970010100 DATE RECEIVED: 02/03/1989 DESC: MBAS OF FC-129, TRITON H66 CONTRACT LAB: PACE CONTRACT LAB COST: 162 3M E-L HOURS: 0 EXP COMP DATE: 02/17/1989 DATE COMPLETED: 02/15/1989 SAMPLES SHOULD BE DELIVERED IN MY NAME TO THE LAB. RUN A MEASUREMENT ON THE STANDARD LAS SOLUTION FOR COMPARISON. DO ALL EXTRACTIONS AND BACK WASH CALLED FOR IN PROCEDURE. FC-129 IS 50% SOLIDS. ITS MV IS 623. THE MV OF THE LAS STANDARD IS PROBABLE ABOUT 325. TEST FC-129 AT A MOLAR CONCENTRATION EQUAL TO THAT OF LAS. G1208-3 IS AN EPA LAS REFERENCE MATERIAL: LOT #1138, AVE. M.V. 318, ACTIVE LAS 6.00 %, PREPARED BY EMSL CINCINNATI US EPA IN COOPERATION VITH SOAP AND DETERGENT ASSOCIATION, DATE 985. Revised report shows correction in project description above. SAMPLE DATE CODE DESCRIPTION RESULT MIN DET LIMIT 1 FC-129 LOT 564 SURFACTANTS - MBAS INORGANIC TESTS REPORT 2200 MG/KG SEE ATTACHED REPORT 2 TRITON H66 RH-RM 6-1536 SURFACTANTS - MBAS 1000 MG/KG 3 LAS STD. EPA LOT 1138 SURFACTANTS - MBAS 60000 MG/KG 4 DUPLICATE #1 SURFACTANTS - MBAS 2800 MG/KG 5 DUPLICATE #2 ........... SURFACTANTS - MBAS 1200 MG/KG 6 DUPLICATE #3 SURFACTANTS - MBAS 60000 MG/KG * = CONTRACT LAB k iSUBMITTED BY: DATED: 5 5 V. 5 5 5 5 000362 fX K C REPORT OF LABORATORY ANALYSIS bboratories,me February 10, 1989 Mr. Hade Schell 3M Environmental Laboratory 935 Bush Avenue Bldg. 2-3E St. Paul, MN 55133 Offices: Minneapolis, Minnesota Tampa, Florida Coralville, Iowa Novato, California &IZ0? V if/S i Dear Mr. Schell: Enclosed 1s the report of laboratory analyses for samples received 02/03/89. If you have any questions concerning this report, please feel free to contact Tom Halverson or me. - Sincerely, Roger C. Splinter, Ph.D. Director, Laboratory Services Enclosures 000363 1710 Douglas Drive North c Minneapolis, MN 55422 Phone (612) 544-5543 an equal opportunity employer poce laboratories, x REPORT OF LABORATORY ANALYSIS Offices: Minneapolis, Minnesota Tampa, Florida Coralville, Iowa Novato, California 3M Environmental Laboratory 935 Bush Avenue Bldg. 2-3E St. Paul, MN 55133 February 10, 1989 PACE Project Number: 890203509 Attn: Mr. Wade Schell G1208 Date Sample(s) Collected: By Client Date Sample(s) Received: 02/03/89 PACE Sample Number: Parameter INORGANIC ANALYSIS INDIVIDUAL PARAMETERS Surfactants (1) Units mg/kg 032110 GI2Q8-1 032120 G12Q.&-2. 032130 Gl 208-3 2200 1000 60000 MDL Method Detection Limit 000364 1710 Douglas Drive North Minneapolis, MN 55422 Phone (612) 544-5543 an equal opportunity employer M et laboratories, me Mr. Wade Schell Page 2 REPORT OF LABORATORY ANALYSIS Offices: Minneapolis, Minnesota Tampa, Florida Coralville, Iowa Novato, California February 10, 1989 PACE Project Number: 890203509 PACE Sample Number: Parameter INORGANIC ANALYSIS INDIVIDUAL PARAMETERS Surfactants (1) Units 032140 J ffil G1208-4 032150 G1208-5 032160 G1208-6 mg/kg 5 2800 1200 60000 MDL Method Detection Limit (1) Analysis performed by EPA Method 425.1. The data contained In this report were obtained using EPA or other approved methodologies. All analyses were performed by me or under my direct supervision. Thomas L. Halverson Inorganic Chemistry Manager * 000365 1710 Douglas Drive North Minneapolis, MN 55422 o Phone (612) 544-5543 an equal opportunity employer ANALYSIS: MBAS jL DATE ANALYZED: -IIpjjS? ANALYZED BY: 737 ^ TIME: . DATA REVIEWED BY: z/ 7 ENTERED BY: 7? rK FOR CLP USE ONLY? T f ^ ^CLIENT NAME: PROJECT NUMBER:_______ PROJECT NAME: ______ FILE #: ____ _ DATE RECEIVED: ______ , DATE COLLECTED: 1 ANALYSIS ------------------------------------------------ :--------------------- SAMPLE TiESULTS UNITS COMMENTS B IK /o.oi S id Oo Z27<} { o.s *9 rtfIt- j<toglmi 7 ^-fo o u j 3 l \ 1 Z2DO too r*l G lZ O fc- ) ml Sample loo m 10 1# ABS 0 .1 5 5 7.00$ 0.0s yg LAS <S 00 & 22- 3 1 ) 2 1,000 " - ~l 1 r T 0.02, to 3113 t'{>0,000 Hi - 3 OrZ -S' 0.30$ 32 N 2600 fly * 2800 \ ~ H \ # O.olo Vo 31 OO 1 0.O1S 3 i 32 tS 3Z U j> IZOO lo 0 ,0 0 0 f * ; v ' *' : ~ 5 ;; 1 * r v 02X13 l i ' s v 7 " ^ - 0 .30S //? - - ]_________ _ 1 \Gto0366 ; .