Document 3pqNoYY4jwD88Bjxa3nKeyrD

A R Z K > - 0 5 l5 MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as P F O A ammonium salt, Ammonium perfluorooctanoate, PFO , FC-116, FC-126, FC-169, FC-143, or as a major component of FX-1003. (Octanoic acid, pentadecafluoro-, ammonium salt, C A S #3825-26-1) Remarks: The 3M production lot number w as 2327. The test sample is FX-1003. It's purity w as not sufficiently characterized, though current information indicates it is a solution of <45% ammonium perfluorooctanoate* 50% water, <3% inert perfluorinated compound and 1 - 2% C5 and C7 perfluoro analogue compounds. The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD: Method: Microbics Microtox "B A S IC " Procedure Test type: Static acute GLP: No Year Completed: 1990 Species: Photobacterium phosphoreum Analytical monitoring: Light loss. Exposure period: 30-minutes Statistical methods: EC10 and EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Not noted. Test conditions: Diluent: 2% NaCI Microbic's Reagent. Test temperature: Not noted. Stock and test solution preparation: A 2000 mg/L stock solution was prepared in Millipore Milli-Q water. Test solutions were prepared using aliquots of the stock solution. Concentrations dosing rate: O nce Stability of the test chemical solutions: Not noted Exposure vessels: Microbic's cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control G038S0 RESULTS Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L Element value: 5-minute E C 50 = >1,000 mg/L 15-minute E C 50 = >1,000 mg/L 30-minute E C 50 = >1,000 mg/L All element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. C O N C LU S IO N S The FX-1003 30-minute E C 50 for Photobacterium phosphoreum was determined to be >1,000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY _____________ ________ Reliability: Klim isch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number G2882. OTHER Last changed: 5/25/00 0038*3- ENVIRONMENTAL LABORATORY MICROBICS MICROTOXR SYSTEM RAW DATA LR Z . A & S o l P& ^ H TEST SUBSTANCE:---- F Y ~ I n C ) ^ TEST DATE: 3 ~ 7 - H O __________ ANALYST: ( 2 &Pcxrd^ Lo 3 < 2 j SAMPLE CUVETTE m--------- -- -- r ~ " INITIAL 1AE "READING! FINAL ASSAY CONCENTRATION l L____________--____ & ,&-- , READING 1(0) 5-MIN. 1(5) 15-MIN. 1 30-MIN. 1(15) 1(30] 81 BLANK CONTROL 1<6 77 68 s? Cl DUPLICATE to n ss~ 73 7 3 B2 log 7 1 61 C2 i> ^ p lic c d c 02 7 g ip b ip ck 63 7> "o log 7 6 6 1 S *\ C3 T)up )i<ra4e )O S' 7 7 1*0 SO B4 ^ o o I I I 7 6 6 3 L*o C4 T>u p l i c a e i O b 7 7 LO sz? B5 )OoO lo g 11 V 6 ' C5 i coAe IO<=( 1*7 li^ cicL s V*o+vc A A Co o c K S o \io . - J o o o r r Q jL BuJU> 1 .0 ry d i ^ 2 . 0 m * 3 * ? . Subs+w w a. cip p e^ty M iic. b lt u h U m A l. ||i - a ' i c M J-n.+ .< a r W= (a. 8 J a j L j w ^ X + p / U . 7 lo rtV > <1, U ^ U J. 00 A f k c i , i o io m lL S w p U . ^ 'W iv V * T '. s i+l<3 C W j -U rliSS T v ', V a b . 'U w ,crob\cs % AiatC >p|a+lofu C038! m . O ttC e n lra + to n s uuer*~ l< .'fit* . f ln a j Lt^T1/ C >^ilf-rA.+io^s. merH/**ct j.bc*v. TnViojdiM r<~ In iKonsAj SnJ. U ser*- 4U-eK. 4*"<*.HS:$/>J {'soJs "** 8 +C . / MICRQTOX(r) DATA SHEET Microto X 5-min exp FX-- 1003 LR 6288 2 3/7/90 sab W b & PAIR # CONC. Io/It 1 125.000 109.0/ 79.0 125.000 108.0/ 78.0 3 250.000 109.0/ 76.0 4 250.000 105.0/ 74.0 5 500.000 111.0/ 76.0 6 500.000 106.0/ 74.0 7 7.1000. 000 108.0/ 65.0 7.1000.000 109.0/ 67.0 BLANK Bo/Bt= 100 / 81 BLANK R A T 10= 0.8100 G-OBS 0. 118 0. 122 0. 162 0. 149 0. 183 0. 160 0.346 0.318 EC 50 = 7.9347.118 t 7.2565.755 TO 7.34051.820 ) R =0.93295 SLOPE = 1.9111 INTERCEPT = +9.1428 G-EST 0.112 0.112 0. 154 0. 154 0.211 0.211 0.289 0.289 /o i It t l*\ 3 la * 0 , Cnobs. * lo 1 &C5 o ) (Ooo o H-------- ---------- V -- ## RES. PROB. 1 -- V-- i o 0. 4 2 0 0.402 3 0.290 4 0. 311 5 0.279 57 53 27 31 25 1 11 1 111 6 0.292 7 0.555 28 -1 > 100 1 < 7. 8 0.502 82 1 1 t111 -2 >11 <1 ---- 3 ---------- 4---- -5--6 ----- 8-. < 1.IJ ' !1.. -- --* 11 1 -3 + ------- 4 5 LOG CONC. 6 7 MI CR OTOX is a R e gi st er ed T r ad e Mark of Microbios Corporation. ) I> 3 3 3> O r 003883 Microtgx 15-min exp. rllCROTOX(r) DATA SHEET FX-1003 LR G2882 3/7/90 sab c# H AIR # CONC. lo/It 1 125. 0 0 0 109.0/ 64.0 2 125.000 w"T 250. 000 108.0/ 66.0 109.0/ 64.0 4 250.000 105.0/ 60.0 5 500. 000 111.0/ 63.0 6 500.000 106.0/ 60.0 7 7.1000. 000 108.0/ 49.0 8 7.1000. 000 109.0/ 52.0 B L A N K B o / B t = 100 / 71 BLANK RATIO 0.7100 G-OBS 0.209 0. 162 0.209 0. 242 0.251 0.254 0.565 0.488 EC 50 7.3347.798 ( 7.1084.118 TO 7.10338.120 ) G-EST 0. 167 0. 167 0.231 0.231 0.321 0.321 0.444 0.444 L o c ^C n H nn Ao o Sto 33 G ~ o ^. X too I + rbS R =0. 90172 SLOPE 1.7282 INTERCEPT = +8.1161 i+ V V - ## RES. PROB. 1 0. 400 o 0.546 3 0. 400 33 "6f2-t 4 0. 348 25 5 0.341 24 6 0.339 24 0 > 7 0.691 100 8 0. 588 72 7. -1 > 8 -. < 4 ----------- 6 1 3---------- -2 + 4 5 LOG CONC. 6 COX>3 2 3>en Q O r MICRQTQX is a Registered Trade Mark of Microbios Corporation. 0038S4 Microtox 30-min exp. iilCRQX (r) D A T A SH E E T FX-1003 LR G2882 3/7/90 sab P 4 PAIR # CONC. lo /It 1 125.000 109.0/ 62. 0 125.000 108.0/ 62. 0 3 250.000 109.0/ 59. 0 4 250.000 105.0/ 56. 0 5 500.000 111.0/ 60. 0 3 5 0 0 . 0 0 0 106.0/ 56. 0 7 7 . 1 0 0 0 . 0 0 0 1 0 8 . '0 / 4 6 . 0 8 7.1000. 000 109. 0/ 4 9 .0 B LANK B o / B t = 100 / 71 BLANK RAT 10= 0.7100. G-OBS 0 .2 4 8 0. 237 0.312 0.331 0 .3 1 3 0.344 0.667 0.579 EC 50 = 7.2918.197 ( 7.1094.210 T O 7.77B2.670 R =0.91498 SLOPE = 2.0386 INTERCEPT = +7.9787 G-EST 0.232 0.232 0.308 O. 308 0.409 0.409 0.544 0.544 I1 3H Ho 31 I -t&rCbii ^ s o > z 00 ## RES. 0. 448 2 0. 480 .ji 0.334 4 0.319 5 0.332 6 0.314 7 0.647 8 0.539 PROB. 48 55 27 24 26 24 100 70 V---------------------------V 7. 8. -1 > 4 6 - 3- 5 1 < ( D i > 3 2 i > c n en o r -2 + 4 5 LOG CQNC 6 7 M I C R Q T O X is a R e g i s t e r e d T r a d e M a r k o-f M i c r o b i c s C o r p o r a t i o n . C-03SS5