Document 3pm4v8ygx8Xr29X8jJ6d6NwE

M AS.6 - 0788 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (SCUD) T EST SUBSTANCE___________________________________________ __ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1 % sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctyl phenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:________________________________________________________ Method: Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms Type: Acute static GLP: No Year completed: 1974 Species: Gammarus fasciatus Supplier: In-house cultures maintained at Bionomics, EG&G, Inc., Wareham, MA. Analytical monitoring: Not given. Exposure period: 48-hours Test organism age: 1 2 - 2 4 hours old fourth instar Statistical method: TL50 (median tolerance limit) values calculated using a linear regression equation. Test conditions: Dilution water: Well water, aged 24 hours Dilution water chemistry: Not given Lighting: Not given Stock and test solution preparation: Not given. Exposure vessels: 250 mL beakers with 200 mL exposure solution Number of replicates: 4 Number of organisms per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: Temperature range (0-48 hours): 20 1 C 001515 RESULTS Nominal concentrations: Blank control, 3200, 4200, 5600, 7500, and 10,000 mg/L. Element values: 48-hour TL50 = 5170 (4190 - 6370) mg/L 48-hour NOEL = 3200 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS____________________________ =______________________ The FC-206 48-hour TL50 was determined to be 5170 mg/L with a 95% confidence interval of 4190 to 6370 mg/L. The 48-hour no observed effect level (NOEL) was 3200 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteriaTor quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_________________________________________ Test was conducted by Bionomics, EG&G, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1974. O T H E R ___________________________________________________________ Last changed: 6/28/00. 001516 BIOASSAY REPORT SUBMITTED TO 3M COMPANY ST. PAUL, MINNESOTA ) V ACUTE TOXICITY OF SAMPLE C TO WATER FLEA (Daphnia magna), AND SCUD (Gammarus fasciatus). Bionomics E G & G, Inc. Environmental Consultants 790 Main Street Wareham, Massachusetts August, 197^ 001517 METHODS AND MATERIALS These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of water flea (Daphnia magna), and scud (Gammarus fasciatus) to Sample C, a brown liquid, v/as evaluated under static bioassay conditions for a ^ - h o u r period. Results were expressed as the median tolerance limit (TL^q ), the nominal concentration of the test compound in water causing 50 percent immobilization for the Daphnia and Gammarus. The TL^q value and its 95%> confidence intervals were calculated by converting the test concentrations and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. r Procedures for static bioassays as described in the Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms were followed, except for those conditions described below. The Daphnia, and Gammarus were acquired from laboratory cultures. The Daphnia and Gammarus bioassays were conducted at 20C (+_ 1.0). The invertebrate tests were conducted in 001518 Page two 2 5 0 ml beakers containing approximately 200 ml of well water which was aged for 24- hours. Five 12-24- hour old Daphnia, and five .fourth instar Gammarus were placed in each beaker (4- replicates/concentration). In the Daphnia bioassays, Daphnia food'*' was added at a rate of 0.5 ml/liter after the introduction of the compound into the test vessels. RESULTS The predicted TL^q values, and 95% confidence intervals for the Daphnia and Gbmmarus are presented in Table 1. Table 2 presents a summary of observed immobilization for each individual test after 24- and 4-8 hours. ^Food was prepared by homogenizing 5 g of starter trout food and 1 g of cerophyl in 100 ml of water. 001519 SUBMITTED BY; Bionomics E G & G, Inc. Environmental Consultants ?90 Main Street Wareham, Massachusetts August, 197^ PREPARED BY: APPROVED BY: Robert E. Bentley fLtrt le- Chief, Acute Toxicit/ Investigations / v Bevier Hasbrouck Sleight, III Freshwater Programs 001520 Table 1 -- Acute toxicity of Sample C to Daphnia3 and Gammarus.^ The data are based on results of bioassays conducted at the fish toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species TL^Q-milligram active ingredient/liter 24 hour 48 hour No Effect Level (mg/l) Daphnia Gammarus > i 0 ,000 5,310(4,340-6,510) 5,850(4,810-7,110) 3,200 5.170(4,190-6,370) 3,200 aBioassay conducted at 20C (_+ 1.0), Daphnia 12-24 hours old at the initiation of tests. ^Bioassays conducted at 20C (+_ 1.0) Gammarus 12-24 hours old at the initia.tion of tests. Q 95$ confidence intervals. 001521 Table 2 -- Concentrations tested and observed percent immobilization for Daphnia and Gammarus exposed to Sample C after 24 and 48 hours. Species Daphnia Gammarus Concentration (mg/l) 10,000 7,500 5,600 4,200 3,200 control 10,000 7,500 5,600 4,200 -3,200 control % immobilization observed 2<Oiaur 48 hour 20 100 25 50 0 40 0 10 00 00 100 80 v 60 45 0 0 100 V 80 75 5 0 0 001522