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FOR DU FONT USE ONLY AR226-2891 Copies Co E. I. du Font do Nemours and Company Haskell Laboratory for Toxicology and Industrial medicine Elitton Road, Newark, Delaware 19711 iemlcaj.5 & Pigmeacs Dapc. Jackson Laboratory EYE IBRITATION TEST IM RABBITS Procedure; One-tenth nillJUiter of undiluted test material was placed into the right conjunctival aac of each of 2 male albino rabbits. After 20 seconds, 1 tred'ced eye was washed with tap water for 1 minute* The treated eye of the other rabbit was not washed. Observations of the cornea, iris and conjunctive were made with an ophthalmoscope at 1 and t* hours, and at 1, 2 and 3 days; Fluor-i-strip stain and a slit-lamp bioiBicroscope were used at examinations after the day of treatment. ot contain TSCACBI Results; Dose Treatment Cornea Ocular Effects Iris Conjuncciva 0.1 ml Not Washed undiluted test material A small area of slight cloudiness 1 br-2 days. None* Redness; Mild 1 hr- 1 day. Swelling;Mild 1-4 hrs. Discharge .'Moderate 1-^ hrs and severe 1-2 days. Hamas tisfS positive 1 hr-2 days. 0.1 ml undiluted cesc material Washed A small area of slight cloudiness 1-4 hrs. Slight cloudiness was seen with the biooicroscope 1-3 days. None. Redness: 1 hr-i day. Swelling;Slight 1-4 hrs. Discharge:None. Summary; t^^^^^^BB^Hj produced a small area of transient slight corneal cloudTnessand mild conjunctivitis with no iritic effects after treatment in both the washed and unwashed rabbit eyes. There was copious discharge in the unwashed rabbit eye. The unwashed rabbit eye was normal within ? days and Che washed eye was normal within 7 days after treatment. This material produced mild ocular effects with severe discharge. In the event of eye contact, flush copiously with water. Synonym; CAS Registry Number: Composition: ^|| Company Sanitized. Does not contain TSCA CBI Report by: ^^ 1^1^ ^ --.J--to--rh--ih--n--n--ER--.---H--H--o--en--n--rrv--y----// Ji Technician Approved by: Chief, Gerald L. Kennsoy Acute Investigarioas Section JEHsjrg acudy Direceor; 0. L. .Date Issued: February _1^------------W^ Report No. 70-81 Dashlell 1?, l98i ,^.0----------------"-- 3 -