Document 3pdBLgKx5XKGB1NDxeeMwr2y
AR 226 _ 0&5
TOXICITY TO AQUATIC PLANTS
IESTSUB~ S~ T~ AN 00C 0000E 000
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m6a0y3EaFls.o (b1eOctanesulfonic acid) (CAS # 2795-39-3).
FRCe-m6a0r3kEsF:. CuTrhreen3tMinpfroordmuacttiioonnilnodtincautmebseitrisisanomtixntoutreed.ofT1h.e30t%estPsFaOmSp,le10is%
diethylene glycol butyl ether, 54.15% water, 22.5% ethylene propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3-
glycol,
2.6%
Chioroally)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0%
triethanolamine, and 0.05% tolyl triazole.
.
The following summary applies a mixture with incompletely
rcehfalreaccttteorxiizcietdyocofnctheentfrlautoirooncsheomfiicmaplurciotimepso.nDeanttaofmtahye ntoetastccsuamrpaltee.ly
METHOD:
0000
Method: OECD Guideline 201
Type: Acute static
GLP: No
Year completed: 1996
SSpoeucricees:: ISne-lhoeunsaestcrulutmurcea.prOirciogimnnaultluymfrom ATCC, Rockville, MD
Element basis: Algal cell counts (cells/mL) and average specific growth
rEaxtpeso.sure period: 96 hours
KStaartbiesrtiMcaelthMoedthaondds:ToxCstoatvsaolfutewsadreetpearcmkiangeed,
by the Trimmed Version 3.5.
Spearman-
Analytical monitoring: pH and temperature.
Test Conditions:
aAlllgmailneNruatlrineunttriMenetdsieusmse:ntPiaelrfOorEaClgDalmegrtohwotdh. aTnhdisalnsuotrsieenrtvemdeadsiuthmepdriolvuiednetd for all algal operations. The pH of this synthetic algal medium was 9.06.
Stock and test solution preparation: A 1000 mg/L primary stock solution __
was prepared by diluting 150 mgof test substance in 150 mL of standard algal nutrient medium. Aliquots were then added directly to dilution water in test vessels to create test solutions.
E50xpmoLsuorfetevsetsssoellutsi:on1a2n5dmcLapbporeodsiwliitchatfeo-aglmassstoEprpleersn.meyer flaskscontaining
002317
Agitation: Number of
Srehpalkiecantecso:nti3nfuooruselxypoastu1r0e0cropnmc.entrations,
6
for
blank
control.
NInuitmiablecrelolflcooandcinegn:tra1t.i0oxns1:0fcievlelpsl/umsL a(1b.l2axnk10conatcrtoulal)
WLiagthetrinCgh:emCiosnttriyn:uous 400 ft-candles using cool-white fluorescent lamps.
TpeHstratnegmepe(r0a-t96urheourrasn)g:e7(.04-896- h9o.u0r6s): 24.2 25.8 C
R0 ESwr0 s 000000
Nominal concentrations: Blank control, 31.25, 62.5, 125, 250, and 500 mg/L
Algal Growth Response EC values
Exposure
(Contact) Hours
Cell-Count mglL (95% C.1
[28 TT30(117-165)|
118583 ((117243--210894,)
Css
147 (123-162
"Average Specific
Growth Rate mglL (95% C.1 171 (146-199 227950 ((225542--330300))
Element values are based on nominal concentrations.
wReevreersdiibliutleidtywiotfh
GarlgoawltmheIdnihuibmitainodn:cAulltiuqrueodtsfoorf
the 500 7 days.
mg/L. test Based on
solution growth
aolbgsisetravtiecd. in the recovery phase, the effect on algal growth was found to be
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetevadlounestrheepmoirxtteudraepapslydetosctrhiatbemdixitnutrheeaTnedstnot the fluorochemical proportion alone.
C0 oncl0 usi0 ons0000000000
mTgh/eLt)esatnsduabs9t6a-nhcoeurexEh/iCbsiotsgrao9w6t-hhorautre
ECs cell value of
count value of 141 (123 204 (265 -- 326) mglL.
--
162
cTohuent96a-nhdou1r2N5omgOlbLsefrorvegdroEwftfhecrtatCeo.ncTehnitsratetsitonsu(bNsOtaEnCc)e iws a3s1.d2e5temrgmliLnefodrtcoell
be algistatic.
--
SPauublm,itMtienrn:esot3aM, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St.
002318
tReesltiianbgi.litHyo:weKvieirmi,sschamrpalnkeipnugri=ty2.waTshinsotstpurdoypemreteytcshatrhaectcreirtiezriead faonr dqutahleitsytudy lacks analytical confirmation of the amount of fluorochemical proportion in the
solution.
This study was conducted by AScl Corporation, AScl-Duluth Environmental Tneusmtbinegr DPi2v5is8i1o,n,1D9u9l6u.th, MN at the requestofthe 3M Company, Lab Request
Last changed: 6/26/00.
002319
BEST COPY AVAILABLE (sT icmsa sios
STupy TITLE GROWTTOH GIRNEHIEBNITAINOLTNGCO3F(%e,FlCAn-GRtOAs3uFEFnF,FcLaIpGrHiTcWoArTnER(TA)
DATA STANDARD OECD GUIDELINE 201
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i
Environmental Testing Division.
| Study Title
`Growth Inhibition of FC-603EF. Light Water
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S(eTlMe)naAsTirCim3%c,opAriRc-oArFnuFuFn)10 Green Alga
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[oaasanwd
[OECD Guideline 201 IM Environmental Technology
and
Services.
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MBuNild$i5n1g332:.3TEe:l09N,o.935(61B2u)sh77A8v-e7n4u5e7. St. Pal.
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SaunsdaSnerBveiaccehs,, IBuMildEinnvgir2o-n3mEe.n0t9a.l9T3e5chBnuoslhogy
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Testing Facility
| |recipe tovesigaor
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[Tesiog FactDirecir {| DDaetfamiViavliedTateisonDaunedsReport Review
Cas
| boniaoum | `NAolavn eMomzbolaer 1996.
| Report Signor: |! is
2 Es
i i RetentionofRaw Data and Final Report
LocationofRawDataandFieyral Report21
A
Two years from the date study is completed.
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|| TAeSsctlinCgorDpiovirsaitoino,n/4A4S4e4l-ADiurlpuatrhk EBnovvilrevoanrmde.nal| +&
Duh, MN S581: Tel. No. 18) 7224040. | [4
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[ "cTooncdeentterramtiinoen t(hNeO4E-Cd).meadnidaanlgeifcfiedcatlcAonlcengtraatieonec(tEC.o.f.iethees4-dsunbosaonbcsee.rsa1bCl-e60efIfEeFct. Ligh | Wcoantdietri(onTsM,) ATC 3%. AR-AFFF for green alga (Selenastrum capricornutim) under static test This study was conducted sccodin to OECD Guideline 201 (OECD 1993)
30 TEST METHODS
[| vest Substance [ Psraobpleer,tieasn:d cAommpbleert-ecloyloSroeldublleiquiind.wastpeercific gravity ca. 1.06 (water = 11. ||
Tet cocenratons: ia msm como, 31.35. 62.5. 138, 250. ang |
Test Organisms | 5So0u0rcmeg:/L.ATCC 23662
I
Andoacyuslubmefproerpa1rastinoni: anAl.gal stock was subcultured in OECD medium for ||
l
Use in est: Stock cular was diluted and spiked ino est solutions to
|
ocbolnaciennatnaiionnit)i.al cell density of 1.0 X 10+ cells per ml (1.2 X10! actual
i)
`Algal Medium
| cPNhr&eempEaiDrcaTatlAio)ann:atloyDswieisilkofinonirzOdeedEiCownaDitzeredewcwaoasmlemsrepniikcseedidnwcRioatnhwcmeDnaiaiaainotPnascs,ktoaTcgkhes.e(mnoelsudrencgent |
+ 2
32
aCuhtaroaccltaevreisptriicosr: toAtusteesitn ttihmee,testt.he medium haad pHof9.06.3 Sterilized via
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ECxhpaomsbuerres | | [ ncatation |
|
Observations
ECnadlpcoulianttions
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Tfyopaem: stSotpepreirlsi.zed 125-mboroslicate lass Erlenmeyer Masks sealed wits | Ussepeliicnateessta.nd50thmrieorfeapllgicaaltemsepdeirutmeostrstuebssttaonlcuetcoonn/ccehnatmrbaetiro.n wSeirxecontrol l eobmspelrovyaetdi.onsDwuerriengmtadhe.testS,olkuetpitonssopwpeerreedn.otexacreapitedwhdeunrinegxptehreimeexnptoaslures. Duration: 4days. DNauiolryespcheonstoplaemrpiso.: "CToenstcihnuaomubserast $e0l0d foincashnadkieeruasbilneg ceotola wh1i0t0epn. |
Temperature; 25+.20C.
| BIimoplrogoivceald:NeDuabialuyercelhlecmooucnyttsomuestinegr direct microscopic cramer
vi
|
aWnadtetrecmhepmiesriray:, 2()1)dAatleysttenmipteraattiuorne.andAltlerGmienramtiionn:atipHo.n cwoenrdeucmtiavdiety according to APHA methods (1993). | a1v-e,r2a-,ge3s.peacinfdic4gdroECw,h/'r.ateasndGs4).-4 ANlgOiEciCdalb/aaslegdisoantibcodtehecremlilncaotuinotns waansd made post toriciy test termination.
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40 RESULTS
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WCeIllACoEunCt 34775ECm:et1(5825m2y30/0L).(173.208) |
|
Coll Count #4 EC, 131 mgr 12162 |
4dECy: 294 mg/L (265-326).
1
|
JCeldl CNOEoC4::4uN1O25nECm:e131.35 mi.
|| "Test Conditions
l5g0i0stmaetc.bcaosnecdenotnrarteicoonvery of cells exposed to Temperature (C): 232.25.8.
|
Light intensiry fi-cdls: 400.
l
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pl: 7,489.06,
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| Specific conductivity umhosiemy: 137-152|.
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39.
6.0 REFERENCES
W`AamteerricaanndPWuabsltiecwaHteearl.lhAAPssHoAci,atWiaosnh(iAngPtAo)n,. D.1C9.95. Sandard Method for he Feammaton or ||
| |
eOrsgtainnigzaotfioCnhefmoircaElcso.noOmEicCDCooPpuebrliactaitoinonanIdnfDoermvaetlioopnmeCnetnte(rO,ECWDa)s.hin1g9t9o3.n.ODE.CC.D
Guidelines
for
| |
|
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CaonncdenRi.rVa.tiTohnusrsitnoTno.xi1c9i7t7.y (3: 417 (1978)
BiToraissmamye.d
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