Document 3eqjG9JKDMeozqavGxZRj5o0x

AR 226-0096 EEF CHRONIC TOXICITY TO EARLY LIFE STAGE OF FISH TESTSUBSTANCE Identity: PFeCr-f9l5u.oro(o1c-tOacnteasnuelsiuolnfaotnei;cmaaciyd,al1s,o1,b2e,2r.e3f,e3r,r4e,d4.t5o,5a,s6,P6,F7O.7S.8o.r8.8heptadecafluoro-, potassium salt, CAS# 2795-35-3) Remarks field: taken from 3M lot nuTmhbeerte2s1t7.subSsatmapnlcee is a white powder. was stored under Sample ambient was conditions prior HMR, "F-NMR atondtesetlinegm.enPtuarlitayndaeltyesremsitneecdhntioqubees.90.49% by LC/MS, 'H- METHOD TMyeptheo:d:FlOowE-CthDro2u1g0h, OPPTS chronic 850.1400 GLP: Yes SYpeeacriecso:mpPlietmeedp:halSetsudpyrcoommepllaested 1999. Report completed 2000 ASunpapllyiteirc:alImno-nhiotuosreincugl:turPeFs,OSWilmdeiiafseuIrnetdernoantidonaayls, Ltd., 0, 4, Easton, MD 7, 14, 21, 28, 35, 42,and 47 Exposure period: 47 days Statistical methods: contingency tables to Discrele-variable identify treatment data were analyzed groups that showed using 2 X 2 a statistically csiognntiifniucoaunts-dviafrfiearbelneceda(tpa<0w.e05r)e fervoamlutahteendefgoartniovremcaolnittryolusgirnogupS.haAplilro- oWfilvka'rsitaenscteaannddfoDrunhnoemtotg'esnteeisttyweorfevaursieadncteouesvianlguaBtaertdleitftf'esrfeensct.es Abneatlywseies.n treatment and control means PTreesttrfeiasthmeangte:: eggs < None 24-hours old at test initiation Test conditions: Dilution Dilution water: water 0.45 um filtered well water chemistry (during the 4-week period immediately preceding the test): hardness: alkalinity: 112762 ((112740--112782)) mmgg//LL aass CCaaCCOO,3 pH: 82 (828.3) ToC: <1.0mglL Conductivity: 321 (315-330) umhosicm CO502 Swattoecrkaatn8d8.t4esmtgs/oLluatnidonmipxreepdaurnattiiloanll: Primary stock test substance prepared dissolved in dilution prior to use. with After mixing, dilution water the primary stock solution was to prepare five additional stock proportionally solutions at diluted cwoenrceenptrreaptairoends eovfe4r4y.2t,hr2e2e.1t,o 1f1o.u0r,d5a.y5s2,daurnidng2.t7h6e mtegs/tL..TShteocsikxssotlouctkisons `wwehreereintjheecytewdeirnetomtihxeeddilwuittehr dmiilxuitinogn cwhataemrbe(ratsa(artataeroafte11of66m.U0mmiLn/umtien)utteo) achieve the Flow dtehsrioruegdhtersattec:onAcpepnrtorxaitimoantse.ly six volume additions of test water every 24-hours Stability of the test Exposure vessels: chemical solutions: SL glass aquaria filled Exiremely stable with approximately 7 L ionfctuebsattsioolnutciuopnswiwtehrae cdoenpstthroufctaepdprforxoimmagltaeslsy c1y7licnmd.ersEmbryo aalptparcohxeidmattoelthye5b0omttmominwidtihasmieltiecronweitshea4la2n5t.umThneylcounpsscwreeerne mesh suspended attached to in a rtohcekewrataerrmcwoiltuhman roefceiparcohcaStLingglmaosstiaoqnuaofrium and approximately 2 rpm. Number of replicates: four Number Number of of fish per replicate: twenty concentrations: six plus a negative control fFiersetd7indga:ysLipvoestb-rhiantecshh.riOmnp dnaauypslii8.thFreodug3ht4i0mepsospte-rhadtacyh,dufreidng3 the ftoirmeats ldeaaislty 4o8n hwoeuerksdparyisoratnodth2ettiemremsindaatiiloynoonfwteesetkteondalsl.owNfootr fgeudt cWlaetaerranccheempriisotrrtyo wdeuirgihntgmtehaesustruedmye:nts. Dissol7v.e6d-o8x.y2gmegnlLra(ncgoentr(o0l-ex4p7odsauyrse)): pH 7.6-8.2mglL (1.2 range (0 - 47 days) mg/L exposure) 8.0-8.4 8.0-8.4 (control exposure) (1.2 mg/L exposure) Test temperature range (0 - 47 days) 24.4-24.7C (control exposure) 24.3-24.7C (1.2 mg/L exposure) Method mean of calculating mean measured concentrations: arithmetic Reswuts NMoemaisnuarledcocnocnecnetnrtartaitoinosn:s: Bk<LcoOnQtr,ol0,.105.,140,.300.,290,.600.,571,2,1.12,.42,.34,.64.m6gm/gLlL Cceso3 Element value: 5-day hatchability NOEC = 4.6 mg/L. 42-day post-hatch survival NOEC = 0.30mg/L 42-day post-hatch growth NOEC = 0.30 mg/L 42-day post-hatch survival LOEC = 0.60 mg/L All element values based on mean measured concentrations Statistical Evaluation of Mortality: The statistical difference for growth at concentrations equal to and higher than 0.60 mg/L was not evaluated due to a significant effect on survival. No statistically significant difference between the negative control and the highest concentration tested was seen forhatchability. Analytical Methodology: Analyses of test solutions were performed at Wildlife International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentration of the test substance in the test solutions, the same and most prominent peak response for perfluorooctane sulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 0.0458 mg/L in this study. The mean percent recovery of matrix fortificationsanalyzed concurrently during sample analysis was 102. Samples collected atpretest ranged from 91.4 to 105% of nominal. Samples at test initiation had measured values from 95.5 to 114% of nominal. Measured values for samples taken at test termination ranged from 95.2 to 111% of nominal. [L5 mnnmideae]e= `Summaryof analytical chemistry data: [m| mecorab|ssmgmmeegeenn[ | || || | |mmmmmmge||| <e|s| h EO heTm sTs e |a|v] Co39.1 Biological observations Hatching success and time to hatch: All viable fathead minnow embryos hatched on Day 4 or 5. There were no`apparent differences between the time to hatch in the negative control and the PFOS treatmentgroups. Survival: All fish surviving to test termination appeared normal with gneoneorvaerltlysaigpnpseaorfesdubtloetbhealswtoiximcmitiy.ng Fish which erratically did not prior to survive death. Gmrgo/wLtfho:r 4F2isdhaeyxsppoossetd-htaotcPhFsOhSowatedconnocesnttartiasttiiocanlslyofsi0g.n1if5icoarnt0.30 reduction in total length, wet weight or dry weight in `comparison to the negative control. Hatchability pCremeT te Tew f[eolfeuTfo ootTa]l Ce CoeTTe eeoe To e[e e ew |eTTa e eooToe o a] Larval Survival [py e eT T] ] CO305; Growth [[eCowme T|ooJJoumm[[meoen]omaen] [on To |werom [wow] ream] I [oe o T |o T[soersaionm[mseernss]]msasssvoonn|| CONCLUSIONS iE ------------ Fathead minnows exposed to potassium perfluorooctanesulfonate at concentrations <0.30 mg/L for 42 days post-hatch showed nostatistically significant reductions in time tohatch, hatching success, survivalor growth. The most sensitive endpoint in this study was post-hatch survival. `PSauublm,itMtienrn:es3oMta,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St, DATAQUALITY Reliability: Klimisch ranking = 1 ees sentnt. REFERENCES ------------ rTehqiusessttudofy twhaes3cMConodmupcatendya.t Wildlife International Ltd. . Easton, MD at the OTHER Last changed: 5/3/00 sec----ee---- ceecos