Document 3eov56MaQ87vDy2ED0MDLZda

i TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER - Z O I - X N (Important -- I f report i s print& ort;bohh -~ Division of paper, send tw copier to TCC.) - Environmental Laboratory (EE&PC), S t Paul, Minnesota Projoct Commercial Chemicals %qioT\TitT Daphnid B i o a s s a y : CS-2151, L o t 3 (LR-6457s) BACK TO MAIN -- . --_ 9970012600 R o p o r t Number 048 . Authortr) h\E M. T. Elnabarawy kotobook Rofmrence Employw Numborfr) 46981 No. of P-r lncludlngCovorrhaot KEYWORDS: (Select terms from 3M Thesaurus. Suggest othei applicable terms. 1 EE & PC Div , (Env. Lab) ' Toxicity (Auat i c ) XJRRENT OBJECTIVE:- To d e t e r m i n e t h e 48 Hour Leso Daphnia magna. of C!S-2151, Lot 3 t o WPORT ABSTRACT: (200250words) Thir abstract iinformetbnu diritributed by the Technical CommunicationsCenter to ilert 3M'ers to Companv R&0. It is Company confidential materiel. Replicate s t a t i c a q u a t i c $&hour LC values o b t a i n e d for daphnids ( D a h n i a maanla) were 78v64-125) and - 7 3 ( 6 2 - 1 0 6 ) ( 6 6 93) mg/l mg/l, combined 48-hour LC50 was 74 Data are p r e s e n t e d a s LC50 values w i t h 95%c o n f i d e n c e limits. 4 -4 . 1)aphnid Bioassay MTE . Paqe 2 February 5, 1981:. BACK TO MAIN In these replicated 48-hour LC50 aquatic tests,, the calculated values for possessing anDaphnia LC50 magna were 76 of this order and 73 mg/l. of magnitude Materials are considered to be slightly toxic based on the NIOSH criteria for aquatic toxicity interpretation (2). This report describes tests performed o'n CS-2151 (light yellow . liquid) to evaluate its acute toxicity to Daphnia e n a . - The report also provides the data generated in thisstudy. ) METHODS AND MATERIALS PI__- a9- The Daphnia ma na culture was obtained from a private hatchery (A). Stock aphnids were held and cared for'at recommended test temperature (21+2OC). A -daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period, was maintained throughout the holding and testing period. Daphnids were fed up . to the beginning of the test with a specially prepared food suspension combination of active dry yeast and fish food (Tetra-Min) (B) First-instar young were segregated 24 hours prior to use in assays. Each 250-ml glass beaker containing 200 ml test solution comprised a test chamber. Twenty (20) Eirst-instar young were introduced into each test chamber within 30 minutes following toxicant addition. The acute short-term static bioassays were performed on CS-2151 using carbon-filtered well water of known composition and constant quality as the diluent (Tables l,2). The studies performed in replicate used the fol.lowing toxicant logarithmic concentrations: 21.75, 28.75, 3 8 . 7 ! 5 , 5 2 . 5 , and 70 mg/l. Mortality or immobility, temperature, dissolved oxygen level, and pH of all test solutions were measured at 24-hour intervals. Monitoring equipment used included ASTM thermometers, Orion temperature-compensating pH meter, a Yellow Springs dissolved qxygen.meter and a Taylor temperature chart recorder. LC50 values with 95% confidence limits were calculated using .the probit program and 3M TRAC Computer system. The test protocol utilized for this study was modeled after that described by ASTM 1980 (1). A. MOgUl-Ed, Oshkosh, Wisconsin. 3. A commercial fish food o f known nutritional composition. - -. Daphnid Bioassay MTE Page 3 February 5, 1981 BACK TO MAIN -R-E-SULTS : In this replicated study, the Daphnia 48-hour LC50 values were 76 and 73 mq/l. Chemicals possessing an LC50 valule in this range are considered to be slightly toxic to the aquatic environment (2) % The data reported herein are based on studies performed by M. T. Elnabarawy and R. R. Robideau. Accompanying data sheets comprise original d a t a . A copy of this report is; filed in the Environmental Laboratory. The reported LC50 values were calculated on the basis of the "observed mortality rates" and of the cadculated initial $' concentrations of CS-2151 in test solutions. N o measurements of the actual concentrations-were made at the end of the 48-hr. bioassay. REFERENCES: (1) ASTM-STandard Practice E729-80. Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macro- . invertebrates, and Amphibians. knerican Society'for Testing and Materials, Philadelphia, PA (1980). ( 2 ) Registry of Toxic Effects of Chemical Substances, ' 7 6 Edition, Christensen, H. E., and E. ,J. Fairchild, Eds., p. X V and Ci, U.S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, DC (June, 1976). MTE/cel BACK TO MAIN TABLE 1 WELL WATER QWLITY SAMPLE DATE: 12/29/80 SAMPLE TAKEN: 3N Bldg. 2-3E, S t . Paul b PARAMETER SAMPfrE DATE 12/29/60 (TAKEN FROM TANK #I) CONCENTRATION ENV. LAB WATER QUALITY BOOK REP. PH R e s i d u a l Chlorine Ammonia Nitrogen Conductivity M-Alkalinity Total S o l i d s 9otal Hardness Total Fluoride Dilute COD Total CoAifom Strndatd Parte Count Aluminum aa A&* Arsenic 88 As* Boron as Cobalt as Cor Copper as Cu* Chromium as Cr* Cadmium as Cd* Iron as Fe* Nickel as N i * Lead as Pbf Silver as ~ g * Zinc as Zn* Mercury as Hg' 7.7 8 <0.02 mg/1 9 (0.1 ng/l as N 8 -340 umhos/cm &t 25OC 9 221 m q / l CIS CaCO3 8 291 mg/l 8 260 mg/l as CaCO3 8 0.04 Htg/l 9 (3.4 mg/l 9 Negativa 10 131 per rl 10 0.01 rg/i 14 <O.QOS ~ 1 8 14 (6.2 mq/1 14 <0.002 mg/l 14 0.018 mg/l 14 < 0 . 0 0 5 mg/l 14 t 0 . 0 0 2 mg/l 14 0.022 mg/l 14 0.005 mg/l 14 <0.005 mg/l 14 <0.002 mg/l 14 0.13 mg/l 14 (0.2 ug/l 14 PCB's** <0.50 ug/l 36 Total Organochlorine** t 0 . 5 0 ug/l 36 Pesticides Total ( 0 . 5 0 uq/l 36 Orqanophosphorus* Pesticides Sample Date 3/31/80 **Sample Date 2/26/80 BACK TO MAIN TABLE 2 CNVI C#)NClENTAL LABORATOII,Y RLffi. 2-3B STI PAUL, MINNESOTA POURTR QUARTERLY REPORT, 1980 WELL WATER SUPPLY FOR AOUA'TIC TOXICITY TESTING PARAMETER TOTAL AARDNeSS* a s mg/l CaC03 TOTAL "M" ALKALINITY* a s mg/l CaCO) 244 240 260 ' 251 2 0 230 214 22L 222 -+ 8 TOTAL SOLIDS* a s mg/l pr1* * STANDARD PLATE COUNT*** per 1.0 m l 324 300 291 305 -+ 17 7.7 7.6 7 . 6 -+ 0 108 139 131 126 -+ 16 - C R I T E R I A OF CONSTANT QUALITY * Monthly ranges should be less than 10%of t.heir respective a v e r a g e s . (ASTM Standard E729-1980.) * * Monthly range of pH s h o u l d be less than 0.4, u n i t . E729-1980.) (ASTM Standard *** Total b a c t e r i a l d e n s i t i e s should n o t be g r e a t e r than 500 organisms p e r m l . (USEPA-600/8-78-017, p . 1 0 1 . )