Document 3eE40QBd9vYnwGRKBykVBJKGO

BACK TO MAIN SANITIZED REPORT , .. ' , EXPLORATORY 28-DAY ORAL TOXICITY STUDY WITH TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS AND PFOS (positive control) BY DAILY GAVAGE IN THE RAT FOLLOWED BY A 14/28-DAYRECOVERY PERIOD NOTOX Project 242933 NOTOX Substances 83484, 83493, 83502, 84456 and 84141 3M Test No. T-7023, T-7024, T-7022, T-7032, T-6295 - page 1 o f 451 - TELOMER ALCOHOL/TELOMER ACRYLATE/ REPORT APPROVAL PFHS/PFOS BACK TO MAIN NOTOX Project 242933 Study Director Ir. S.C.M. de Hoog . . n Management Drs. W.J.A.M. Frieling - Page 2 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ CONTENTS PFHS/PFOS NOTOX Project 242933 . REPORT APPROVAL SUMMARY PREFACE ; STUDY PLAN MATERIALS AND METHODS ' Test Substances Test Substance Formulation Purpose and Rationale Guidelines Arch iving Test System Allocation Animal Husbandry Treatment Recovery Observations Mortality / Viability Clinical Signs Body Weights Food Consumption Water. Consumption C inical Laboratory Investigat ons Haematology Clinical Biochemistry Ur ina 1ys is Patho 1ogy Necropsy Organ Weights Liver Sampling Histotechnology Histopatho logy Statistical Analysis RESULTS Observations Mort a1 ity Clinical Signs I- 2 5 '7 8 9 9 10 11 11 11 12 12 13 13 13 14 14 14 14 14 14 14 15 16 17 17 17 18 18 18 19 19 20 20 20 20 - Page 3 - BACK TO MAIN .. TELOMER ALCOHOL /TELOMER ACRYLATE/ PFHSlPFOS NOTOX Project 242933 RESULTS (continued) 21 Body Weight Food Consumption Clinical Laboratory Investigations Haemato 1ogy Clinical Biochemistry Pathology Macroscopic Examination Organ Weights Microscopic Examination Discussion and Conclusion 21 21 22 22 23 24 24 . 24 25 25 FIGURES Body Weights Body Weight Gain Food Consumption Relative Food Consumption - TABLES SUMMARY DATA Clinical Signs Body Weights Body Weight Gain Food Consumption Relative Food Consumption Haematology Clinical Biochemistry Organ Weights -Organ/Body Weight Ratios Historical data Haematology / Clinical B i ochemistry -27 28 29 30-31 -32-33 34 35 36 -37 60 61 -64 -65 68 -69-72 73 76 -77 88 -89 100 - 101 112 113-118 TABLES - INDIVIDUAL DATA Mortality C1 inical Signs, Daily Body Weights Body Weights Gain Food Consumption Relative Food Consumption Key to Missing Values and Remarks Haematology I 119 - 120 125 - 126 152 - 153 160 - 161 168 -169 172 - 173 184 Clinical Biochemistry Haematology Clinical Biochemistry Macroscopic Findings Organ Weights-Organ/Body Weight Ratios 185 -186 221 222-257 - 258 281 - 282 305 APPENDIX 1 Pathology Report LAST PAGE OF REPORT -1 146 45 1 - Page 4 - TELOMER ALCOHOLlTELOMER ACRYLATE/ - PFHS/ PFOS BACK TO MAIN NOTOX Project 242933 SUMMARY Exploratory 28-day oral toxicity study with Telomer Alcohol , Telomer Acrylate , PFHS and PFOS (positive control) by daily gavage in the rat followed by a 14/28-day recovery perdod. Dose levels for this study, provided by the sponsor, were selected to be 0 (Vehicle Control), 3 (PFOS), 1 0 (PFHS) and 30 rng/kg body weightlday (Telomer Alcohol, Telomer Acrylate 1. The study was based on the following guidelines: - EEC Directive 96/54/EEC, B.7 Repeated Dose (28 days) Toxicity .(oral), 1996. - OECD 407, Repeated Dose 28-day Oral Toxicity Study in Rodents, 1995 The test substance, formulated in 1% aqueous carboxymethyl cellulose, was administered daily for at least 28 days by oral gavage to SPF-bred Sprague Dawley rats. One vehicle control group and five treated groups were tested, each consisting of 8 males and 8 females. An extra 3 animals per sex per group were allowed 14 days of recovery and another 3 animals per sex per group were allowed 28 days of recovery. The following parameters were evaluated: clinical signs twice daily (treatment) or once daily (recovery); body weight twice weekly; food consumption weekly; clinical pathology after 4 weeks and at the end of both recovery periods; mtaicsrsousecso.py at termination; organ weights and histopathology on a selection of RESULTS Te1omer A cohol: (30 mglkg day) Te 1omer A rylate: (30 mglkglday) (30 mglkglday) 1. Total protein, globulin and total cholesterol were decreased (a) after treatment. Total protein was still decreased after 2 and 4 weeks recovery (a). 2. Midzonal/centrilobular hypertrophy was observed in the liver, and exocrine apoptosislsingle cell necrosis in the pancreas (3)after treatment and had partially or completely resolved after 4 weeks recovery. 1. Body weights were decreased at the end of treatment ( 9 ) 2. Total protein, globulin and total cholesterol were decreased (a)and albumin/globulin ratios were increased (a) after treatment. 1. Total protein, globulin, aspartate aminotransferase activity (a) and prothrombin time (8) were decreased and albumin/globul in ratios were increased (a) after . treatment. Prothrombin weeks recovery (8) time was still decreased after 2 - Page 5 - BACK TO MAIN .. TELOMER ALCOHOL/TELOMER ACRYLATE] PFHSlPFOS NOTOX Project 242933 PFHS : (10 mg/kg/day) PFOS : (3 mg/kg/day) 1. Total cholesterol (a and 8), total -protein, globulin, triglycerides, aspartate aminotransferase activity (6)and prothrombin time (8and 8 ) were decreased; albumin/globulin ratios and alkaline phosphatase activity were increased (a)after treatment. Prothrombin time was still decreased after 2 weeks recovery (8). 2. Liver weights were increased after treatment (8). 3. Midzonal/centrilobular hypertrophy was observed in the liver (a) after treatment and had completely resolved after 4 weeks recovery. 1. Body weights and food consumption were decreased during treatment and recovery ( 8 ). 2. Total protein, globulin, total cholesterol, triglycerides, aspartate aminotransferase activity (a) and prothrombin time (5') were decreased; albumin/globulin ratios were increased (8)after treatment. 3. Autopsy body weights were decreased (8). Liver weights were increased after treatment and recovery (8and ? ) . 4. Midzonal/centrilobular hypertrophy was observed in the liver ( 8 and 8) after treatment and remained in evidence among males after recovery. From the results presented in this report it can be concluded that the positive control compound PFOS dosed at 3 mg/kg/day was indeed the compound with the most striking and persistent effects, mainly in the liver. Compounds with comparable but less severe effects as PFOS included PFHS (10 mg/kg/day) and TELOMER ALCOHOL (30 mg/kg/day). Despite slightly reduced body weights in females (TELOMER ACRYLATE only) and some slight changes in the blood after treatment, 30 mg/kg TELOMER ACRYLATE or per day did not result in clear systemic toxicity and organ dysfunction at macroscopic or microscopic level. I - Page 6 - TELOMER ALCOHOL/TELOMER ACRYLATE/ - .. PFHSIPFOS BACK TO MAIN NOTOX Project 242933 PREFACE .. Sponsor Study Monitor Invoice Contact Testing Facility Study Director Histopathology 3M Toxicology Services 3M Center, B,ldg 220-2E-02 St. Paul, MN 55144-1000 USA , Marvin T. Case, DVM, PhD 3M Toxicology Services 3M Center, B l d g 220-2E-02 St. Paul MN 55144-1000 Telephone (651)-733-5180 Fax (651)- 733-1773 Barbara Nelson 3M Procurernent Operation s 3M Center, Bldg 224-lN-04 St. Paul, MN 55144-1000 Telephone (651)- 733-0766 Fax (651)- 733-1799 NOTOX B.V. Hambakenwetering 3 5231 DD 's-Hertogenbosch The Netherlands Ir. S.C.M. de Hoog Dr. J. Th. Wilson ... - Page 7 - .. e BACK TO MAIN TELOMER ALCOHOL /TELOMER ACRYLATE/ 'PFHS/PFOS NOTOX P r o j e c t 242933 STUDY PLAN S t a r t of Treatment 06 October 1998 (males) 08 October 1998 (females) S t a r t Recovery 04 November 1998 (males) 06 November 1998 (females) Blood sampling 03 November 1998 (Main groups, males) 04 November 1998 (Main and Recovery groups 1 and 2, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1 , males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2 , males) 04 December 1998 (Recovery groups 2, females) Urine collection predose day 7 day 7 day 14 day 14 end of treatment end of treatment end of recovery end of recovery end of recovery end of recovery : 29-30 : 11-12 : 13-14 : 18-19 : 20-21 : 03-04 : 05-06 : 17-18 : 19-20 : 01-02 : 03-04 September 1998 October 1998 October 1998 October 1998 October 1998 November 1998 November 1998 November 1998 November 1998 December 1998 December 1998 (Recovery (Recovery (Recovery (Recovery (Recovery (Recovery (Recovery (Recovery (Recovery (Recovery (Recovery groups groups groups groups groups groups groups groups groups groups groups 1 and 2) 1 and 2, males) 1 and 2, females) 1 and 2, males) 1 and 2, females) 1 and 2, males) 1 and 2, females) 1, males) 1, females) 2, males) 2, females) Necropsy 03 November 1998 (Main groups, males) 04 November 1998 (Main groups, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main groups, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) d 04 December 1998 (Recovery groups 2, females) - Page 8 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS MATERIALS AND METHODS NOTOX Project 242933 TEST SUBSTANCES The sponsor is responsible f o r the completeness of all test substance data. All available information on characteristics of the test substance were taken into account before the start of the study in order to determine if they conflict with/might affect the proceeding of the study. NOTOX SUBSTANCE 83484 Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Specific Gravity Stability in vehicle TELOMER ALCOHOL TPa- 7l0e2t3o medium tan waxy solid Not indicated by sponsor; treated as 100% pure At.room temperature in the dark Stable 07 September 2000 1.7 1% aqueous carboxymethyl cellulose: not indicated NOTOX SUBSTANCE 83493 Identification Description . Batch Purity Test substance storage Stability under storage cond it ions Expiry date Density Stability in vehicle TELOMER ACRYLATE -Yellow liquid T 7024 Not indicated by sponsor; treated as 100% pure At room temperature protected from light Stable 8 07 September 200 Approx. 1.6 g/cm at 50 'C 1% aqueous carboxymethyl cellulose: not indicated NOTOX SUBSTANCE 83502 Identification Description. Batch Purity Test substance storage Stability under storage conditions Expiry.date Stability in vehicle -White powder T 7022 Not indicated by sponsor; treated as 100% pure At room temperature in the dark Stable 07 September 2000 1%aqueous carboxymethyl cellulose: not indicated - Page 9 - .. c BACK TO MAIN TELOMER ALCOHOL lTELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 NOTOX SUBSTANCE 8445618 Identification PFHS - f Description Off-white crystalline solid Batch Purity Test substance storage T 7042 Not indicated by sponsor; treated as 100% pure At room temperature in the dark Stability under storage conditions . Stable Expiry date 25 September 2000 Stability in vehicle 1% aqueous carboxymethyl cellulose: not indicated NOTOX SUBSTANCE 84141 Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date - Stability in 'vehicle PFOS -White solid T 6295 Not indicated by sponsor; treated as 100% pure At room temperature in the dark Stable 07 September 2000 1% aqueous carboxymethyl cellulose: not indicated Veh icle Rationale for vehicle Stability in vehicle 1% aqueous carboxymethyl cellulose Based on trial formulations performed at NOTOX Stability over 4 hours will be determined by the sponsor TEST SUBSTANCE FORMULATION Method Formulations (w/w)were prepared daily within 4 hours prior to dosing. No adjustment was made for specific gravity of the test substance. Storage conditions At ambient temperature No chemical analysis of dose preparations was performed. - Page 10 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHSlPFOS NOTOX Project 242933 PURPOSE AND RATIONALE The nature and purpose of this exploratory study was to determine and compare the relative oral toxicity in rats following oral (gastric gavage) doses administered daily over 28 days and identify developmental compounds of lesser toxicity for commercial development, This study should provide part of a rational basis for toxicological risk assessment in man. The oral route was selected as it is a possible route of human exposure during manufacture, handling or use of the test substance. GUIDELINES The protocol was reviewed and agreed by the Article 14-functionary and the Ethical Committee of NOTOX as required by the Dutch Act on Animal ' Experimentation. The study procedures described in this report were based on the following guidelines: 1) EC Directive 96/54/EEC, Annex IV.D, replacing EEC Directive 67/548/EEC, Part B : Methods for the Determination of Toxicity and other Health Effects; B.7: 'Repeated Dose (28 days) Toxicity (oral)'. Official Journal o f the European Communities No. L248, September 1996. 2) OECD Guideline for Testing of Chemicals, Guideline 407: 'Repeated Dose 28-day Oral Toxicity Study in Rodents', Paris Cedex, 27 July 1995 The protocol, study procedures and the report were not inspected by the NOTOX Quality Assurance Unit. ARCHIVING .. NOTOX B . V . , will a r c h i v e the following d a t a for at least 10 years: protocol, raw data, report and all specimens. No data will be withdrawn without the sponsor's written consent. - Page 11 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE] PFHS/PFOS NOTOX Project 242933 TEST SYSTEM Test System Age at Start of Treatment Number of anima S Randomisation Identification Sprague Dawley rat Cr1:CD BR (outbred, SPF-Quality) Recognised by international guidelines as the recommended test system (e.g. EPA, FDA, OECD, EEC). Females were nulliparous and non-pregnant. Source : Charles River, Sulzfeld, Germany Approximately 6 weeks. 84 males, 84 females At least 5 days before study start, by computergenerated random algorithm according to body weight, with all animals within F: 20% o f the sex mean. A health inspection was performed prior to commencement of treatment to ensure that the animals were in a good state of health. Earmark and tattoo ALLOCATION Group Identification 1 Main group 1 Recovery group 1 1 Recovery group 2 2 Main group 2 Recovery group 1 2 Recovery group 2 3 Main group 3 Recovery group 1 3 Recovery 'group 2 4 Main group 4 Recovery group 1 4 Recovery group 2 5 Main group 5 Recovery group 1 5 Recovery group 2 6 Main group 6 Recovery group 1 6 Recovery group 2 Test Substance Number of animals males females Vehicle control 8 8 Vehicle control 3 3 Vehicle control 3 3 Telomer Alcohol 8 8 Telomer Alcohol 3 3 Telomer Alcohol '3 3 Telomer Acrylate 8 8 Telomer Acrylate 3 3 Telomer Acrylate 3 3 8 8 3 3 .3 3 PFHS 8 8 PFHS 3 3 PFHS 3 3 PFOS (positive control) 8 8 PFOS (positive control) 3 3 PFOS (positive control) 3 3 Animal numbers males females 1- 8 9-11 13-15 17-24 -25 27 29-31 -33 40 41-43 -45 47 -49-56 57 59 61-63 -65 72 -73 75 -77 79 81-88 -89-91. 93 95 -97 104 - 105 107 109-111 113-120 - 121 123 - 125 127 - 129 136 137-139 - 141 143 - 145 152 - 153 155 - 157- 159 161 168 169- 171 173-175 - 177- 184 185 187 189-191 Test substances Telomer Alcohol (substance 83484), Telomer Acrylate (substance 83493) and (substance 83502) were tested at a dose level of 30 mg/kg body weight /day. PFHS (substance 84456) was tested at a dose level of 10 mglkg body weightlday. PFOS (substance 84141) was tested at a dose level of 3 mg/kg body weightlday. - Page 12 - BACK TO MAIN TELOMER ALCOHOLITELOMER ACRYLATE/ 'PFHSIPFOS NOTOX Project 242933 ANIMAL HUSBANDRY Conditions Air-conditioned room with approximately 15 air changes per hour and the environment controlled with optimal conditions considered as being a temperature of 21C and a relative humidity of 50%. Lighting was 12 hours artificial fluorescent light and 1 2 hours dark per day. Deviations from these optimal conditions occurred, but were considered not to have affected study integrity. Accommodation Group housing o f 3-4 animals per sex per cage in stainless steel suspended cages with wire mesh floors. Acclimatisation period was at least 5 days before start of treatment under laboratory conditions. Diet Free access to standard pelleted laboratory animal diet (from Carfil Quality BVBA, Oud-Turnhout, Belgium). Each batch is analysed for nutrients and contaminants are analysed on a regular basis. Results are examined and archived. Water Free access to tap-water. Certificates of analysis (performed quarterly) were examined and archived. TREATMENT Method Frequency Dose volume Dose level Oral gavage, using a stainless steel stomach tube, Formulations were placed on a magnetic stirrer during dosing. Once daily for at least 28 days, approximately the same time each day, 7 days per week. Main group animals were dosed up to the day prior to necropsy 5 mllkg body weight, Actual dose volumes were calculated weekly according to the latest body weight. Group Group Group Group Group Group 1: 0 mglkg b.w./day 2: 30 mglkg.,b.w./day 3: 30 mglkg' b.w./day 4: 30 mglkg b.w./day 5: 10 mglkg b.w./day 6: 3 mg/kg b.w./day (Vehicle Control) (Telomer Alcohol) (Telomer Acrylate) 0 (PFHS) (PFOS, positive control) RECOVERY Duration Recovery group 1: at least 14 days Recovery group 2: at least 28 days - Page 13 - ~ e ~~~ .. BACK TO MAIN TELOMER ALCOHOLlTELOMER ACRYLATE/ PFHSlPFOS NOTOX Project 242933 OBSERVATIONS Mortality / Viability Clinical signs Body weights Food consumption Water consumption Twice daily. During the treatment period detailed clinical observations were made twice daily in all animals: once immediately after dosing and once approximately 4 hours after dosing. During the recovery period only one observation per day was performed. The time o f onset, degree and duration were recorded. All symptoms were recorded and graded according to fixed scales: Maximum grade 4: grading slight ( 1 ) to very severe (4) ' Maximum grade 3: grading slight ( 1 ) to severe ( 3 ) . Maximum grade 1: presence is scored ( 1 ) . Treatment period : on days 1, 5, 8, 12, 15, 19, 22, 25 and 28. Recovery period : on days 1, 5 , 8 , 12, 14, 19, 22, 25 and 28. Weekly. Subjective appraisal was maintained during the study, but no quantitative investigation introduced as no effect was suspected. CLINICAL LABORATORY INVESTIGATIONS Blood samples were collected under light ether anaesthesia, between 7.30 and 9.30 a.m. immediately prior to post mortem examination for Main and Recovery group animals, and for Recovery group animals also at the end of the treatment ' period. The animals were fasted overnight (with a maximum of 20 hours) before blood sampling, but water was provided. Blood samples were drawn from the retroorbital sinus of all Main and Recovery ratslsexlgroup and collected into tubes prepared with EDTA for haematological parameters (0.5 ml), with citrate for clotting tests (1.O ml) and untreated tubes for clinical biochemistry parameters (>2.0 ml). From animal numbers 17-20, 33-36, 49-52, 65-68, 81-83, 113, 115, 131 and 132 a second EDTA-sample was collected after 4 weeks, due to inexplicable clotting of the first sample. - Page 14 - . BACK TO MAIN .. TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 HAEMATOLOGY The following haematology parameters were determined from blood prepared with EDTA as an anti-coagulant, using the instruments listed: Parameter/Abbreviation Unit Instrumentation Erythrocyte countlRBC HaemoglobinlHB T/l mmo1/1 Sysmex K-1000 Sysmex -K 1000 Haematocrit/HCT 111 Sysmex K-1000 Mean corpuscular volumelMCV f1 Sysmex K-1000 Mean corpuscular fmol haemoglobinlMCH Mean corpuscular haemoglobin mmo111 concentration/MCHC Sysmex K-1000 Sysmex K-1000 Platelet count Gll ' Sysmex K-1000 Red cell distribution w i dt h /RDW % Total leucocyte count1WBC 611 Sysmex K-1000 Sysmex K - 1000 Differential leucocyte count/SEG (Neutrophils) l(re1) Man ua 1 (Microscope) I EO (Eosinophils), BAS0 (Basophils), LYMPH (Lymphocytes), MONO (Monocytes) I The following haematology parameters were determined from blood prepared w i t h citrate as an anti-coagulant, using the instruments listed: ParameterlAbbreviation Unit Instrumentat ionlMethod Prothrombin time/PT sec Partial thromboplastin time/PTT sec Sysmex CA-5000 Sysmex CA-5000 - Page 15 - BACK TO MAIN .. TELOMER ALCOHOLITELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 CLINICAL BIOCHEMISTRY The following clinical biochemistry parameters were determined from serum samples using the instruments listed: Parameter/Abbreviation Unit Instrumentation Alanine aminotransferase/ (ALAT / GPT ) pkat/l Epos select ve analyser 5060 (Eppendorf ) Aspartate aminotransferase/ pkat/l ( ASATIGOT) Epos select ve analyser 5060 (Eppendorf) , Bilirubin, total/ BILI T. pmolll Epos select ve analyser 5060 (Eppendorf) Cholesterol, total/ , ' CHOLEST. T. mmo1/1 Epos selective analyser 5060 (Eppendorf) Triglycerides/ TRIGL. mmo1/1 Epos selective analyser 5060 (Eppendorf) Creatinine pmolll Epos selective analyser 5060 (Eppendorf) Glucose mmo / I Epos se.lective analyser 5060 (Eppendorf) Urea mmo /l Epos selective analyser 5060 (Eppendorf) Protein, total/ PROTEIN T. g/ 1 Epos selective analyser 5060 (Eppendorf) Protein, albumin/ 911 ALBUMIN . .' Protein, globulin/ gll GLOBULIN Epos selective analyser 5060 (Eppendorf) Calculation [PROTEIN T.-ALBUMIN] Albumin Globulin ratio 1 A/G RATIO Cal cu 1at ion [ALBUMIN/GLOBULIN] Alkaline phosphatase/ ALP pkatll Epos selective analyser 5060 (Eppendorf) Sodium mmol/l EFIX-5055 Flame Photometer Eppendorf Potassium mmol/l EFIX 5055 Flame Photometer Eppendorf Chloride mmo1/1 Jenway PCLM3 Calcium mmol/l EFIX 5055 Flame Photometer Eppendorf Phosphorus/ INORG. PHOSPH mmol/l . Epos selective analyser 5060 - Page 16 -(E.ppendorf) .. d BACK TO MAIN TELOMER ALCOHOL /TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 URINALYSIS (Recovery group animals only) Urine samples were collected overnight (approximately 16 hrs) from all animals assigned to the Recovery groups according to the following schedule: - predose - during the treatment period on days 71 1 4 and at the end of treatment - at the end of the recovery period, prior to scheduled necropsy. During the sampling period animals were deprived of food but water was provided. Urine was collected into a specimen vial, using a metabolism cage. After collection the samples were frozen and stored at -20Cat NOTOX until further analysis. PATHOLOGY NECROPSY . All animals surviving to the end of the observation period were deeply anaesthetised using ether vapour and subsequently exsanguinated. All animals assigned to the study were necropsied and descriptions of all macroscopic abnormal it ies recokded . Prior to exsanguination a blood sample (1-2 ml) for serum collection was taken from the aorta. Serum samples were stored at -20C at NOTOX until further analysis. Inadvertently, no serum sample was collected from all Main males and females from groups 1, 2, 3 and 6 (animal numbers 97-100, 113-116, 129-132 and 177-180). Instead, reserve samples intended for clinical biochemistry might be used for possible further analysis. Samples o f the following tissues and organs were collected from all animals at necropsy and fixed in neutral phosphate buffered 4% formaldehyde solution: I Identification marks Adrenal nlands Aorta Brain Caecum Co 1on Duo den um Epididymides Eyes with optic nerve and Harderian gland Female mammary gland area Heart Ileum Jejunum Kidneys L i ver Lung, Lymph niondfeussed- with formalin mandibular, mesenteric Oesophagus Ovaries Pancreas - Page 17 - TELOMER ALCOHOL /TELOMER ACRYLATE/ * ._ PFHS/ PFOS BACK TO MAIN NOTOX Project 242933 Peyer's patches (jejunum, ileum) if detectable Pituitary gland Prostate gland R e c t um Salivary glands - mandibular Sciatic nerve Seminal vesicles Soinal cord -cervical, midthoracic, lumbar sp 1 een Sternum with bone marrow Stomach Testes Thymus Thyroid including- .parathyroid Trachea : Urinary bladder 1 Uterus All gross lesions ORGAN WEIGHTS The following organ weights (and terminal body weight) were recorded from the surviving animals on the scheduled day of necropsy: Adrenal Brain Heart Kidneys Liver Ovaries Sp 1 een Testes glands Both absolute and relative (% body weight) organ weight data are included in the study report. LIVER SAMPLING From all animals surviving until the scheduled necropsy at least 1 gram, of the left lateral liver lobe was frozen in isopentane/Np. Liver samples were stored at -2O'C at NOTOX and on 01 February 1999 dispatched on dry ice to the sponsor. Analysis is performed under the responsibility o f the.sponsor. Results may be reported by the sponsor-in a separate document. -. HISTOTECHNOLOGY All underlined organ and tissue samples (see under Necropsy) from all Main and Recovery group animals were processed, embedded and cut at a thickness of 2-4 micrometers and stained with haematoxylin and eosin. - Page 18 - BACK TO MAIN .. TELOMER ALCOHOL/TELOMER ACRYLATE1 'PFHS/PFOS NOTOX Project 242933 HISTOPATHOLOGY Slides of all underlined organs and tissues (see under Necropsy) collected at the scheduled sacrifice from all Main group animals of all dose groups as well as from all animals of all dose groups which died spontaneously, and all gross lesions of all animals (all dose groups) were examined by a pathologist. Based on treatment related morphologic changes, the following organs were also examined from all rats of Recovery proups 1 and 2: -- liver from males of the Vehicle control, Telomer Alcohol, PFHS and PFOS groups; liver from females of the Vehicle Control and PFOS groups; - pancreas from males of the Vehicle Control and Telomer Alcohol groups. All abnormalities were described and included in the report. I STATISTICAL ANALYSIS ! The following statistical methods were used to analyse the data: Univariate one-way analysis of variance was used to assess the significance of intergroup differences. , If the variables could be assumed to follow a normal distribution, the Dunnetttest (many-to-one t-test) based on a pooled variance estimate was applied for the comparison o f the treated groups and the control groups for each sex. The Steel-test (many-to-one rank test) was applied instead o f the Dunnett-test when the data could not be assumed to follow a normal distribution. All tests were two-sided and in all cases p 0.05 was accepted as the lowest level of significance. Group means were calculated for continuous data and medians were calculated for discrete data (scores) in the summary tables. Test statistics were calculated on the basis of exact values for means and pooled variances. Individual values, means and standard deviations may have been rounded off before printing. Therefore, two groups may display the same printed means for a given parameter, yet display different test statistics values. References : - C.W. Dunnett A Multiple Comparison Procedure for Comparing Several Treatments with a Control, J . Amer. Stat. Assoc. 50, 1096-1121 (1955). - R.G. Miller Simultaneous Statistical Inference, Springer Verlag, New York (1981). - Page 19 - TELOMER ALCOHOLlTELOMER ACRYLATE/ RESULTS - PFHS/ PFOS BACK TO MAIN NOTOX Project 242933 OBSERVATIONS MORTALITY No treatment-related mortality occurred during the study period. One female receiving TELOMER ACRYLATE died following blood sampling, prior to necropsy. This was considered to be an accidental death. CLINICAL SIGNS TELOMER ALCOHOL Rales were noted in one male immediately and 4 hours after dosing. This was observed at the end of the treatment period until the third week of recovery. TELOMER ACRYLATE A hunched posture was observed in two Main group females in weeks 2 and 3 of the treatment period and in two Recovery group females at the end of treatment and during the first week(s) of recovery. Piloerection was also observed in the one female during the first week of recovery. In general the findings were observed immediately and 4 hours after dosing. A hunched posture was observed immediately and 4 hours after dosing in one Main group female from week 3 until the end of the treatment period. - P F H S A hunched posture was observed immediately and 4 hours after dosing in one male at the end of treatment until the second day of recovery. A similar finding was seen 4 hours after dosing in one Main group female on one occasion at t h e end o f treatment. - P F O S A hunched posture was observed in two Main group females in weeks 3 and 4 of treatment. A swelling on the abdominal skin was observed in one Main group male at the end of the treatment period. Other findings such as alopecia, salivation, a missing tail apex, red discolouration of the skin, wounds, scabs, scales, fissures with bleeding on the - apnadwlso,rswcoenltlrionlg aonfimtahles.eaTrheansde feiynedaibnngosrwmearleiticeonsswiedrereednott eod baemoonfg nothteotxriecaotleodgical significance as incidences remained within the range of biological variation for rats of this age and strain or could be attributed to the blood sampling procedure. .. - Page 20 - BACK TO MAIN .. TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 BODY WEIGHT TELOMER ALCOHOL Body weight gain of males was decreased at the end of the recovery period when compared to the corresponding control values. Absolute body weights of these animals remained however within the range of control values. TELOMER ACRYLATE Body weishts of females were slightly decreased when comDared to control values at the etid of the treatment period. - - PFHS Body weight gain of males was decreased at the end of the recovery period. Absolute body weights of these males were also below control values, but did not differ with statistical significance. - PFOS Body weights and body weight gain of females were decreased with statistical significance from week 2 of treatment until the end of the recovery period. Statistical significance was achieved in the majority o f cases, mainly for absolute body weights. Among males, body weight gain was reduced with statistical significance in weeks 3 and 4 of the recovery period. Absolute body weights of these males were also below control values, but did not differ with statistical significance. Body weights and body weight gain of animals receiving remained in the same range as controls over the treatment and recovery period. FOOD CONSUMPTION - PFHS Absolute food consumption among males was slightly reduced at the end of the 4- week recovery period. However, after correction for body weight this effect was not observed anymore. - PFOS Absolute food consumption of females was reduced from week 3 of treatment until the end of recovery. This effect was less apparent after allowance for body weight. Among males, absolute food consumption was slightly reduced at the end of the 4week recovery period, but relative food consumption was within control values. -. There were no differences in food consumption before or after allowance for body - weight between animals receiving TELOMER ALCOHOL, TELOMER ACRYLATE or and control animals. - Page 21 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 CLINICAL LABORATORY INVESTIGATIONS HAEMATOLOGY TELOMER ALCOHOL After 4 weeks, red blood cell count, haemoglobin and haematocrit values were decreased in females. After 2 weeks recovery, prothrombin time was decreased in females. After 4 weeks recovery, red cell distribution width was decreased in males. TELOMER ACRYLATE After 4 weeks, total red blood cell count was slightly decreased in males. This decrease was no apparent anymore after the 2- and 4-week drug-free recovery periods. After 4 weeks, differential leucocyte count revealed decreased relative values of neutrophilic granulocytes and increased relative lymphocytes values in males. Partial thromboplastin time was increased in males of this dose group. Prothrombin time was decreased in females. After 2 weeks recovery, prothrombin time was still decreased in females. After 4 weeks recovery, red cell distribution width was decreased in males. - PFHS After 4 weeks, total red blood cell count, haemoglobin, haematocrit, total number of platelets and prothrombin time were decreased in males. Total red blood cell count, haematocrit and prothrombin time were decreased in females. After 2 weeks recovery, prothrombin time and total platelet numbers were decreased in females. After 4 weeks recovery, no statistically significant differences when compared to controls were observed. - PFOS After 4 weeks, mean corpuscular haemoglobin was increased in males and haematocrit and prothrombin time were decreased in females. A f t e r the 2- and 4-week drug-free recovery periods no statistically Significant differences were observed. - Page 22 - TELOMER ALCOHOL/TELOMER ACRYLATE/ .- PFHS/ PFOS BACK TO MAIN NOTOX Project 242933 CLINICAL BIOCHEMISTRY TELOMER ALCOHOL After 4 weeks, total cholesterol, total protein and globulin values were decreased in males. After 2 weeks reco-very, total protein was still decreased in males. After 4 weeks recovery, total cholesterol, total protein, potassium and calcium values were decreased in males. TELOMER ACRYLATE After 4 weeks, total cholesterol, total protein and g-lobulin were decreased and albumin/globulin ratios were increased in males. After 2 weeks recovery these differences were not apparent anymore. After 4 weeks recovery, total protein, potassium and calcium values were decreased in males. After 4 weeks , aspartate aminotransferase activity, total protein, globulin and potassium values were decr.eased and albumin/globulin ratios were increased in males. After 2 weeks recovery, glucose values were increased and sodium values were decreased in males. Urea was increased in females. After 4 weeks recovery, total protein, inorganic phosphate, potassium and calcium values were decreased in males. PFHS After 4 weeks, aspartate aminotransferase activity, total cholesterol , triglycerides, total protein and globulin values were decreased and albumin/globulin ratios, alkaline phosphatase activity and chloride were increased in males. Total cholesterol was decreased and chloride values were increased in females. After 2 weeks recovery, no statistically significant differences were observed anymore. After 4 weeks recovery, total cholesterol, glucose and potassium values in males were below control values with statistical significance. _ PF_ OS. After 4 weeks , aspartate aminotransferase activity, total cholesterol, triglycerides, total protein and globulin values were decreased and albumin/globulin ratios and chloride were increased in males. Urea and chloride values were increased in females. After 2 weeks recovery no such findings were observed. After 4 weeks recovery, aspartate aminotransferase activity, total bilirubin, total.protein, potassium and calcium values were decreased and chloride was increased in males. - Page 23 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ `PFHS/PFOS NOTOX Project 242933 PATHOLOGY MACROSCOPIC EXAMINATION Macroscopic observations at necropsy did not reveal any alterations that were considered to have arisen as a result of treatment. The few macroscopic findings recorded at all sacrifices were unremarkable and d i d not distinguish treated rats from the vehicle control group. ORGAN WEIGHTS - PFHS After 4 weeks, liver weights and 1iver:body weight ratios were increased in males. After 2 and 4 weeks recovery, this effect had disappeared. Increased brain: body weight and testes: body weight ratios observed after the 4week recovery period is attributed to the slightly low autopsy body weights of these animals. PFOS After 4 weeks, autopsy body weights were decreased in females. Liver weights and 1iver:body weight ratios were increased in males and females. Kidney:body weight ratios were increased in females. After 2 weeks recovery, liver weights and 1iver:body weight ratios were still increased in males. After 4 weeks recovery, 1iver:body weight ratios were increased in males and females and kidney:body weight ratios were increased in females. Other changes noted in PFOS treated animals included decreased absolute heart weights and increased brain:body weight ratios in females after 4 weeks of treatment and 2 weeks-recovery. In addition, absolute spleen weights were increased in males after 2 weeks recovery and testes:body weight ratios w e r e increased after 4 weeks recovery. All these changes were likely to reflect slightly low or high body weights at autopsy. Organ weights and organ:body weight ratios of animals treated with TELOMER ALCOHOL, TELOMER ACRYLATE or were considered to be similar to those of control animals. - Page 24 - TELOMER ALCOHOL/TELOMER ACRYLATE/ a PFHSIPFOS BACK TO MAIN NOTOX Project 242933 MICROSCOPIC EXAMINATION (see Appendix 1) TELOMER ALCOHOL After 4 weeks, midzonal/centrilobular hypertrophy was seen at a minimal or slight degree in six males. In the pancreas a slight increase in the incidence of minimal or slight degrees of exocrine apoptosislsingle cell necrosis was recorded in six males. After 2 and 4 weeks recovery, the liver changes had partially or completely resolved and the pancreas findings returned to the level of the vehicle control group. - PFHS After 4 weeks, midzonal/centrilobular hypertrophy was seen at slight to moderate degrees in all males. After 2 weeks recovery, this finding had partially resolved. After 4 weeks recovery, this finding had completely resolved. - PFOS After 4 weeks, midzonal/centrilobular hypertrophy was seen at minimal to moderate degrees in all males and females. After 2 and 4 weeks recovery, this finding had partially or completely resolved in females, however remained in evidence in males. The remainder of the microscopic findings recorded after treatment and recovery were within the range of background pathology encountered in Sprague Dawley rats o f this age ad strain and occurred at similar incidences and severity in both control and treated rats. DISCUSSION AND CONCLUSION Following daily oral administrations o f TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS or PFOS to male and female Sprague Dawley rats for at least 28 days, treatment-related changes were most pronounced in the TELOMER ALCOHOL, PFHS and PFOS groups. Hepatocellular hypertrophy i n the liver together with higher liver weights was seen in animals receiving PFHS and PFOS. In the TELOMER ALCOHOL group the hepatic hypertrophy was not accompanied by increased liver weights. This liver enlargement is most likely related to the higher enzymatic activity in metabolising a xenobiotic compound. Among males receiving PFOS this effect was rather persistent since it was still observed at the end of the 4-week drugfree period. Minimal to slight exocrine apoptosis/single cell necrosis was recorded in the pancreas of males receiving TELOMER ALCOHOL for 4 weeks and had resolved after the recovery period. - Page 25 - .. TELOMER ALCOHOL lTELOMER ACRYLATE/, 'PFHSlPFOS BACK TO MAIN NOTOX Project 242933 Investigations of the blood showed that various parameters were slightly reduced in one or both sexes after treatment. The most characteristic decreases were: - total protein and globulin in all dose groups; - total cholesterol in all dose groups except for the group; - triglycerides in the PFHS and PFOS groups; - aspartate aminotransferase activity and prothrombin time in the 1 PFHS and PFOS groups. In the majority of cases these changes were not observed anymore after a 2-week drug-free period. A possible explanation for these changes could be liver damage as the liver is involved in the synthesis and metabolism of these compounds. However, the only microscopic change noted in the liver was hepatocellular hypertrophy in the TELOMER ALCOHOL, PFHS and PFOS groups only. Moreover, it should be noted that the majority of values remained within the range of values that can be expected for animals of this age and strain. Changes in clinical biochemistry parameters noted after 4 weeks of recovery but not after 2 weeks were considered to have occurred by chance. At this time point in the study it seems very unlikely that they represent an effect of treatment. Fluctuations in electrolyte levels were seen at all time points in all dose groups. Moreover, urea levels and kidney weights were increased after treatment in females receiving PFOS. However, in the absence o f evidence of renal dysfunction at microscopic level these changes are considered to be of little toxicological relevance. Haematological examination revealed slightly decreased red blood cell counts, haemoglobin and/or haematocrit values in animals treated with TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS and PFOS. However, since the effects were slight in nature and no further evidence of an anaemic response was obtained at macroscopic or microscopic level, the toxicological relevance of these findings is doubted. Other haematological changes noted were not supported by corresponding signs of toxicity and again the toxicological impact is considered to be of little relevance also because the values were within the range of historical data. An effect on body weight and food consumption was most pronounced among females receiving PFOS. This effect lasted until the end of the recovery period. Body w e i g h t s o f f e m a l e s r e c e i v i n g TELOMER ACRYLATE w e r e s l i g h t l y reduced at t h e e n d of treatment. Other effects on body weight gain and food consumption were mainly seen at the end of the recovery period and therefore no toxicological relevance is attached to these findings. Clinical signs such as a hunched posture, piloerection andlor rales were observed incidentally in all five dose groups in a few animals only. At this low incidence and the severity observed these symptoms are considered not to represent a clear indication of toxicity. From the results presented in this report it can be concluded that the positive control compound PFOS dosed at 3 mglkglday was indeed the compound with the most striking and persistent effects, mainly in the liver. Compounds with comparable but less severe effects as PFOS included PFHS (10 mglkglday) and TELOMER ALCOHOL (30 mglkglday). Despite slightly reduced body weights in females (TELOMER ACRYLATE only) and some slight changes in the blood after treatment, 30 mglkg TELOMER ACRYLATE or per day did not result in clear systemic toxicity and organ dysfunction at macroscopic or microscopic level. - Page 26 - TELOMER ALCOHOL/TELOMER ACRYLATE/. PFHS/PFOS BACK TO MAIN NOTOX Project 242933 FIGURES 27 NOTOX Project 242933 BODY WEIGHTS MALES GRAM rREATMNT 40C :ECOVERY BACK TO MAIN - BW-SPLT 1 08-DEC-98 300 200 100 6 0 I I I I I I I 'I 8 WEEKS NOTOX Project 242933 BODY WElGHTS FEMALES GRAM lXEATMENT IRECOVERY BACK TO MAIN BW-SPLT - 2 08-DEC-98 100 0 0 2 4 I 6I 1 8I I WEEKS - - - - - - - - .-----.....-GG-RROOUUPP 1 2 WEHICLE (TELOMER CONTROL) ALCOHOL) GROUP 3 (TELOMER ACRYLATE) -------------- -.-l---l--- GROUP 4 GROUP 5 (PFHSI GROUP 6 (PFOS) 29 NOTOX Project 242933 BODY WEIGHT GAIN MALES x RREATMENT 100 lECOVUiY 80 60 40 BACK TO MAIN BWG-SPLT - 1 08-DEC-98 0- 0 2 4 6 - - - - - - - - ------.---CGRR-OOUU-PP.21-(TV-EELHOICMLEERCAOLNCTORHOOLL)) ----------- ----- CROUP 3 (TELOMER ACRYLATE) . GROUP 4 GROUP 5 (PMS) -------I- GROUP 6 (PFOS) 30 I 8 WEEKS NOTOX Project 242933 BODY WEIGHT GAIN FEMALES - 'REATMENT - 100 - 80 - 60 - lECOVERY BACK TO MAIN BWG-SPLT - 2 08-DEC-98 0 1 I 1 1 I 1 1 I I 0 6 8 WEEKS - - - - - - - - GROUP 1 (VEHICLE CONTROL1 -.----..--G-R-OU-P.2.(TELOMER ALCOHOL) GROUP 3 (TELOMER ACRYLATE) -I-*------- ------------- GROUP 4 GROUP 5 (PFHS) GROUP 6 (PFOS) 31 NOTOX Project 242933 FOOD CONSUMPTION MALES WANIMAVDAY :REATMENT .. . BACK TO MAIN FC-SPLT - 1 01-MAR-99 lECOVERY I 1 I I I 1 I I I 2 4 6 8 WEEKS NOTOX Project 242933 FOOD CONSUMPTION FEMALES WANIMAVDAY rREATMWT lECOMRY BACK TO MAIN FC-SPLT - 2 0 I-MAR-99 . l 1 I 2 I I I I I 6 8 WEEKS NOTOX Project 242933 RELATIVE FOOD CONSUMPTION MALES G/KG BODY WEICHT/DAY 'REATMENT 120 ECOVERY BACK TO MAIN - RFC-SPLT 1 0 1-MAR-99 ' * 100 80 60 40 20 -.. 0 I 0 I. I 2 I I 4 I Q I i I WEEKS - - - - - - - - ----.---CG-RROO-UUPP-21-((-TVEE-LHOIC-MLEER CAOLNCTORHOOLL)) -.--------- GROUP GROUP 3 4 (TELOMER ACRYLATE) ----- GROUP 5 (PMSI -----.I-- GROUP 6 (PFOS) 34 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION FEMALES WKG BODY WEIMT/DAY 'REATMENT 120 lECOVERY . I 100 80 60 BACK TO MAIN RFC-SPLT - 2 0 1-MAR-99 20 0, 1 I I I I I 1 I I 2 4 6 8 WEEKS GROUP 1 (VEHICLE CONTROL) ---.-1-*--1---- ------------------------ ------.-- GROUP 2 (TELOMER ALCOHOL) GROUP 3 (TELOMER ACRYLATE) GROUP 4 GROUP 5 (PFHS) GROUP 6 (PFOS) , 35 / TELOMER ALCOHOLlTELOMER ACRYLATE,' PFHS/ PFOS BACK TO MAIN NOTOX 242933 TABLES - SUMMARY DATA 36 BACK TO MAIN NOTOX Project 242933 SYM-SUM 1 7 -MAR -- 1 9 9 CLINICAL SIGNS (-Y) MALES GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GRADE) WEEKS: 1..............2 . . . ..........3 .............4" ............5. DAYS:. 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ALOPECIA ( 3 ) ' (FORELEGLEFT) G: %: .................................................................... l1l1l1l1l1ll1l1l1l1.1........................... ......... 0: %: ........................................................1111111111111111111111111111111111111.1..................... G: %: .................... ~..........llllllllllllllllllllllllllllllllllll.. 111111111111111111111111111111111111.. G: %: ....1111............................................................................................................ G: %: ......11121212121212121212121211111111111l1l1lllllllllllllllllllllllllllllllllll.................................... G: %: . . ........111111111111111111111111111131313111113111111111~1111111.1..1............................................ G: %: . . ........11111111111111111111111111111111111111111111111111111111111111111111.................................... G: %: .. ............................111111111111lllllllllllllllllllllllllllllllllllllll.l.l.l............................ .G: %: .................................................................................................................... G: . %: .................................................................................................................... .... G: Median value of the highest individual grades %: Percat of affected animals (0 = less than 5%, 1 = between 5% and 15%, Time after treatment (H.Mi) A: 0.00 E: 4.00 A = mre than 95%) NOTOX P. r o. j . e - c.-t 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GF!ADE) LO2ATION RECOVERY wws: 1......2......3......4......5. G: %: ............2122222222222222.............................. G: %: 1112...................................................... G: %: 1121....................................................... BACK TO MAIN - SYM-SUM 2 25 -MAR- 99 ... G: %: Median value of the Percat of affected highest animals individual daily grades ( 0 = less than 5%, 1 = betwea 5% and 15%, .... A = more than 95%) 38 NOTOX Project 242933 -- BACK TO MAIN - SYM-SUM 3 17-MAR-9 9 CLINICAL SIGNS (SUMMARY) MALES GROUP 2 (TELOMEEt ALCOHOL) SIGN (=.GRADE) TREAmmr WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. m: G: ...................................................... .111 %: .......................................................lll G: %: .............................................................. 11111111111111111.1................................... G: ..........................................lllll........... %: ..........................................lllll........... G: %: ............1111111111111111111111111111111111111111111111 ............1111111111111111111111111111111111111111111111 0: ....'..................................ll.................. %: .......................................ll.................. G: %: ......................................................;.....t.........................1.1.1.11.111111111............ G: %: .................................................................................................................,1..~ G: ..................-...............,............l....-.~-.. %: .............................................,.l.......... G: ........................................11......1111...... %: ........................................ll......llll...... G : ...............................................l.......... %: ...............................................l.......... G: %: .................................................................................................................... G: %: ................................................................................................................... . .... G: Median value of the highest individual grades %: Percent of affected anifials (0 = less than 5%, 1 = between 5% and 15%, T h e after treatment ( H . d ) A: 0.00 B: 4.00 39 A = m r e than 95%) NOTOX Project 242933 CLINICAL SIGNS, DAILY {SUMMARY). MALES GROUP 2 (TELOMER AIX3OHOL) SIGN (MAx.GRADE) LOCATION RECOVERY WEEKS: 1......2......3......4......5. G: %: .. 1111111111111111..1111..... ..;.... 1222222222222223..3333 G: %: 112121212.1................................................ G: %: 112:1..................................................... G: %: ..............2l2l2l...................................... G: %: ..........21.............................................. BACK TO MAIN - SYM-SUM 4 25-MAR-99 G: %: M e d i a n value of the Percent of affected highest Mimals individual daily grades ( 0 = less than 5%, 1 = between 5% and IS%, .:. , A = mre than 95%) 40 NOTOX Project 242933 CLINICAL SIGNS (SUMMARY) MALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN .. - SYX-SUM 5 17-MAR-9 9 S I a (MAX.GRADE) WEEKS: 1.............2.............3.............4....*........5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.~.1. TIME: 3 G: %: ............ ..............................llllll...................... ;.................111111...................... G: %: 1111111121212111111111111111111111111111............................................................................. G: %: .................... 11122222221111111111lll1llllllllllll...................... 11111111111111111111llllllllllllllll.. G: %: 111111212212121221111111111111111111.1..1.1.1....................................................................... 0: ..................................... .11.. .1111.. ......... %: ......................................ll...llll........... G: ...........................................lllllllllllll.. %: ...........................................lllllllllllll.. G$3:: .................................................................................................................... ... G: Median value of the highest individual grades %: Percent of affected animals ( 0 = less than 5%, 1 3 between 5% Md 158,. T h after treatmat (H.Mi) A: 0.00 B: 4.00 41 A = more than 9 5 % ) NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 3 ("EL- ACRYLATE) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1......2......3......4......5. S..IC..IN.../..F.U..R../..P..L.U.MPGE RED ST- (3) (NECK) G: %: ............................................313131313131.. BACK TO MAIN - SYM-SUM 6 - 2 5 -MAR 93 G: Median value of the highest individual daily grades %: Percent of affected animds ( 0 = less than 5%, 1 = between 5% 42 is%, .... A = more than 95%) NOTOX Project .242933 - - .. BACK TO MAIN - SYM-SUM 7 - 17-MAR 99 CLINICAT; SIGNS (SUMMARY) MALES GROUP 4 SICS (MAX.GRADE) .... TRExmEm WEMS: l......... 2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: G: %: ..........................:........................................................................................... G: 8: .......................................................................................................... .. ll... 11. G: %: ................................................................ 1l1l1L1t1l11l1l1l1l1l1.l.l.......................... G: %: .......................................... llllllllll................................................................ G: %: .................................................................................................................... G: %: .................................................................................................................... 0: %: .................................................................................................................... G: %: .................................................................................................................... .... G: Median value of the highest individual grades %: Percent of affected anbmls (0 = less than 5%. 1 = between 5% and 15%, Time after treatment (H.Mi) A: 0.00 B: 4.00 43 A = more than 9 5 % ) NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROW 4 SIGN (=.GRADE) LCCATTON r WEEKS: .... RECOVERY 1.. 2......3..,...4.... ..5. s..K..I.N../.m../.P..L..u.M...A.G E ALOPECIA (3) (EYE RIGHT) G: %: ........................12121213131313131313131313131313.. EYE RIGHT, INNRCD (1) G: 1 1 1 1 1 ~ ~ ~ ~ ~ ~ ~ 1 1 ~ 1 1 ~ 1 1 1 1 1 1 1 1 1 1 . %: 1222222222222223333333333333. G: .22222....................... %: .22222....................... 0: %: 112121212121.............................................. G: %: 213121212121.............................................. BACK TO MAIN - SYM-SUM ,o 25-MAR- 99 c G: %: Median value of the highest Percent of affected. animals individual daily grades ( 0 = less than 5%, 1 = between 5% and E%, .... A = more than 95%) 44 BACK TO MAIN NOTOX Project 242933 - SYM-SUM 9 17-MAR-39 CLINICAL SIGNS (SCIMMARY) MALES G R O U P 5 (PFHS) SIGN (MAX.GRADE) TxEamEw WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.1.1.2.3.4.5.6.1.1. TIME: G: %: ............:..... ......................................................llll ....................................llll G: %: ................................................l.ll........................,.1..1..1............................... G: %: ..................1111111111111111111111............................................................................. G: ....................................................ll.... %: .....................................................ll.... G: %: .................................................................................................................... G: %: .................................................................................................................... 0: %: .................................................................................................................... G: M d a n value of the highest individual grades %: Percent of affected animls (0 = less than 5%, 1 il between 5% Theafter treatment (H.bfi.1 A: 0.00 B: 4.00 45 is%,.... A = m r e than 95%) .. NOTOX P r o j e c t 242933 CLINICAL SIGNS, DAILY (SUMMARY) . MALES GROUP 5 (PFHS) SIc;N (MPX.GRADE) LOCATION RECOVERY WEEKS: 1......2......3.,....4......5. G: 11............................ %: 12........................... 0: %: ..............2ll2........................................ G: %: ..................2~21212~2121313133131313131313133131.11. G: %: l1l2,................................................... .. BACK TO MAIN SYM2 -5 S-UMMAR-- 9t 9o G: %: Median value of the Petcent of affected highest mhd.s individual daily grades ( 0 = less than 5%, 1 = between 5% and 15%, .... A = more than 95%) 46 BACK TO MAIN NOTOX Project 242933 CLrNICAL SIGNS ( m y ) MALES GROUP 6 (PFOS) SYM-SUM -;I 17-MX3-9 9 SI- (MAX.GRADE) WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.1.1.2.3.4.S.6.7.1.2.3.4.5.6.7.1. TIME: G: ..........................l~llllllllllll......~........... %: ..........................llllllllllllll.................. G: .................................lllllll.................. %: .................................lllllll.................. 0: .%: .................................................................................................................... G: Median value of the highest individual grades %: Percent of affected Mkrals (0 = l e s s than 5%, 1 = between 5% Time dfter treatment (H.Mi) A: 0.00 B: 4.00 47 is%, .... A = mre t h 95~%) - NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES . GROUP 6 (PFOS) SIc;N (MAX.GRADE) LaA!rION RECOVERY WEEKS: 1......2......3.......4......5. S-K--IN---/-F-U--R--/--P-L-UM-A-G-E- RED STAINING (3) (HEAD) G: %: .......................................313131313131313131.. BACK TO MAIN SYM-SUM -12 2 5 -MAR- 9 9 .. . G: %: Median value of the Percent of affected highest animals individual daily grades (0 = less than 5%, 1 = between 5% Md 15%, .... 48 A = more than 95%) BACK TO MAIN NOTOX Project 242933 - SYM-SUM 13 17-MAR-99 CLINICAL SIGNS (SUMMARY) FEMALES GRom 1 (VEEIICLE CONTROL) SIGN (MAX.GRADE) ...... ... ... TP.EAnEm WEEKS: l....... 2.............3.......... 4.......... 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: G: %: ...............;..........;......................;................................................................... G: %: .................................................................................................................... G: %: ..............................................................11.1111111111111111............................... ..... G: %: .................................................................................................................... G: 1111222211111111111111111111111111111111111111111111111111 %: 1111111111111111111111111111111111111111111111111111111111 G: %: .............................................................................. iiiiiiiiiiiiiiiiiii 1111111111111111111 G: %: ...........111111111111111.1111111111111111111111111111111 ...........111111111111111.1111111111111111111111111111111 G: %: ........................................................... ...... 1111111111111111111111. ..... 1111111111111111111111.. G: %: ......11111111111111111111.1,1................................................. ...111111111111111111....... ......... G: %: 1111111111111111111111111111.........................................11111111111111111111111111111111..........111111 G: %: ........................................................................ lllllllllllllll....... lllllllllllllll....... G: %: ...................................................................................................... 11111111111111 G: %: .................................................................................................... 11.............. G: %: ................................ll......................................................................... ......... G: %: .................................................. 1111111111111111111111111111111.. 111111111111111111111~111111111.. .. G: Median value of the highest individual grades %: Percent of affected animals (0 f less than 5%, 1 = between 5% and IS%, ,, A = more than 9 5 % ) Time after treatment (H.Mi) A: 0.00 B: 4.00 49 NOTOX Project 242933 CLmCAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 1 (VEBICLE CONTROL) SIGN (MAX.GRADE) LCCATION WEEKS: . RECOVERY 1......2 . . . . . 3 . . . . . .4... . . . 5 . s..K.I.N../.F..u.R../.P.L..u.M..A. GE ALOPECIA (3) (GENERAL) G: %: ..................................3131313131313131313131 G: %: ...............................3131...................... G: %: ............2121212121212121213131...................... G: %: ....................21212121............................ G: %: ............2121212121212121213131...................... G: %: 1121212121212121212121212121213131...................... G: %: 1112122222222222232323232222222323...................... G: %: ......212121212121212121212121.......................... G: %: 112121212121212121212121212121.......................... 0: %: ......21212121212121212121.............................. BACK TO MAIN SYM-SUM -.i4 2 5 -MAR-9 3 G: 0: Median value of the Percent of affected highest -1s individual daily grades (0 = less than 5%, 1 = between 5% and 15%,.... A = mre than 95%) 50 NOTOX Project 242933 BACK TO MAIN - . . SYX-SUM I5 17-MAR- 0 9 CLINICAL SIGNS (SUMMARY) FEMALES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) TREA?MENT WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: 3 S-K-I-N-/--F-U-R-/--PL-U-M-A-C-E---.. ALOPECIA (3) (HEAD) G: %: ................................................................................................................... . G: ..........~............................ll~lll~l~l......... %: .......................................llllllllll......... G: %: ................................................................................................~.l.............. 11 G: %: ............................................................................................ ............1111111111 G: %: ........ ........ ......... 11111111111111111 11111111111111111 I........... l.................... ...................... 0: %: ............................................................................................. lllllll112.. 1111111111.. G: %: .................................................................................................................... G: %: ........................ .......................................................................................... G: %: ........................ ..........................................l1l1............................................ G: %: ......................... l............................................ l..........................................., .... G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% & 15%, T h e qfter treatrent (H.Mi.1 A: 0.00 B: 4.00 A = more than 95%) . 51 - NOTOX Project 242933 CLINICAL SIGNS, DAILY.(SUMMARY) FEMALES GROUP 2 ("EL- ALCOHOL) s1m (MAx.GRADE) LdcATIoN RECOVERY WEEKS: 1......2......3......4......5. G: %: ..............22222222221212.... ........................ ............ G: .111222222222'2.. %: .2222333333333.............. G: %: ........3132323232323323232.2........................... G: %: ..............12121212121212............................ G: %.: ..............12121212121212............................ BACK TO MAIN SYM-SUM 2 5 -MAR -- 2 6 9 9 G: %: ~ e d i a nvalue of the pe&ent of affected highest Mimals individual daily grades (0 = less than 5%, 1 = between 5% and 15%, .... 52 A = mre than 95%) BACK TO MAIN NOTOX Proiect 242933 - SYM-SUM 17 17-MAR- 43 CLINICAL SIGNS (SUMMARY) -FEMALES GROUP 3 (TELOMES AcRyI;ATE) SIGN (MAX.GRADE) ...... ...... TREATMENP WEEKS: l....... 2.............3....... 4*............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: G: %: ........................;...................1l.l1ll1l1ll1l1ll~l~ll1...~..~..1...1......................11111111111111 0: %: ................................................................................................................... . G: 1111222211111111111111l11111112122222211111111111111111111 %: 1111111111112222111111111133321111111122222232222222211111 G: %: .................................................. llllllllllllllllllllll............................................ G: %: .............................................. llllllllllllll...,.................................................... G: %: .............................................................................. ll.............:.. ll................. . G: %: ................................................................................................................ 11 11 G: %: ........................................................................................................ 111111 111111 G: %: ...................................................11.1111........................................ 1111111.. 1111111.. G: %: .....................................................................................ll.llllllllll.................- I G: %: ................................................111111111111111111111111.1.1.~...............1.11.1..1..1..1.1...... G: %: .................................................................................. 111111111111111.. 1111111~1111111.. G: Median d u e of the highest individual grades %: Percent of affected anirrnls (0 t l e s s than 5%, Time After treatment (H.Mi) A: 0.00 8: 4.00 53 1 = between 5% and 15%,.... A = more than 95%) NOTOX Proiect 242933 * .. CLINICAL SIGNS, DAILY (.SUMMARY) FEMALES GROUP 3 (TELOMER ACRYLA'I'E) SIM (MAX.GRADE) LKATION WEEKS: ..... RECOVERY 1. 2......3......4... .. 5 . G: %: 2131313113131312121212121212............................ 0: .1111....................... %: . 2 2 2 2 . . . . . . . . . . . . . . . . . . . . . . . G: %: 111212121212121212121121212.2........................... G: %: .................................................... 11 77 BACK TO MAIN SYM-SUM -.18 25 -MAR-99 ,, -. G: %: Median d u Percent of e of.the affected highest animals individual daily grades (0 = less than 5%, 1 = between 5% and 15%..... A = more than 95%) 54 NOTOX Project 242933 CLINICAL SIGNS CxIMMmY) FEMALES GROUP 4 .. c BACK TO MAIN - SYM-SUM l g 17-MAR-99 SIGN (MAX.GRADE) WEMS: DAYS : 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ALOPECIA ( 3 ) G: %: ........................i............;.. .. .. ................1111111111111111111111111111111111l1l1l1l1l1l1l1l1l1l1l1 G: %: ........................11111111111111111111111111.................. .................................................. G: %: ..................................111111111111111111111111......11111111111111111111111111 .... ....1111111.. 1111111.. G: %: ............................................1111111111111111111111111111111111111111111111111111~112121212121212121 G: %: ............................................212121212121212121212121212121212121212121212121212121212121212111111111 G: %: ..............................................111111111111111111111111.1.1.......................................... G: %: .................................................................................................................... G: %: .................................................................................................................... G: %: ..................................................................................................................... G: %: ............................................111111111111111111....................................................... G: %: ............1111111111111111111111111111111111111111111111 ............1111111111111111111111111111111111112211111111 WATERY DISCHARGE E'RCM EXE (3) (EYERIW) . G: %: .................................................................................................................... .... G: Median value of the highest individual grades .. %: Percent of affected animals (0 = less than 5 % , 1 = between 5% and 15%, A = mre than 95%) Time after treatment (H.Mi) A: 0 . 0 0 B: 4.00 55 NOTOX Proiect 242933 CL3cNICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 4 SIGN (MAX.GRADE) LOCATION RECOVERY WEMS: 1......2......3......4 ......5. S..K.I.N.../..F.U..R../...P.L.U.MAGE SCABS (3) RED ST- (3) (PERIORBITALREGION RIGHT) G: %: .................................................... 1. 3. G: %: ......l2l2..... .. .. .. .. .:. . . . ... .. ..... ..... ..... ....... G: %: ..........2l2l....................31313131.............. s..E.c..R.f.i.I.O..N./...E.x.c..R.f.iIchl WAmY FRDM ME (3) G: ..............ll............ D1SCHARC;E (EYE RIGHT) %: ..............23......... ... BACK TO MAIN - SVM-SUM 20 25 -MAP- 99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5 % , 1 = b e m e n 5% and 15%,..., 56 A = more than 95%) NOTOX P r o j e c t 242933 - .. BACK TO MAIN SYM-SUM - 21 CLINICAL SIGNS FEMALES GROUP 5 (PFHS) WEEKS: T1R.E.A.T.M.E.N.T......2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIMI?.: P-o------- IIuNcHeD WSrcTRE (1) m .../.F..u.R../.p..L..u..M..p..I ; E ALOPECIA (3) (BAM) ALOPECIA (3) 1-( G: %: ..........................;............................................................................1......... -1.. G: %: ............................................ 1111111111111112111111111111............................................ G: ..11111111122222211111lllllllllllllllllllll12222222222222.. %: ..111111111111111111113333333344333322222222211111111111.. G: %: ............1111111111222222222222222222222222111111111111 ............1111111111111111111111111111111111111111111111 G: ............-...........................,..............l.. %: .......................................................l.. G: %: ..................................................................................................................... G: 1111112222111111111111222222111111111111111111111111111... %: 11111111111111111111lllllllllllllllllllllllllll~lllllll... G: %: ................1111....111111111112121212121111.................................................................... G: %: .......l.................................................. .......l.................................................. , ... G: Median value of the highest individual grades %: P e r c e n t of affected animals (0 = less than 5%, 1 = between 5% Md is%,. Time after treatment (H.Mi) A: 0 . 0 0 E: 4.00 A = mre than 95%) 57 `NOTOX P r o i e c t 242933 CLINICAL SIGNS, DAILY (.SUMMARY) FEMAtES G R O U P 5 (PFHS) SIGN (MAX.GRADE) LmATIoN WEEKS: ..... RECOVERY 1. 2......3......4......5. s.K..I.N../.F.u.R../.P.L..u.M..A..GE ALOPECIA ( 3 ) (BACX) G: %: .................................................... 11 33 G: . 111. iiiiiiiiiiiiiiiiiiiiill %: 122..22222333?37777777777777 G: %: .... ..... 111 111111111111111111111 122 222222223333333333333 G: %: ............12122121.e .................................. BACK TO MAIN - SYM-SUM 22 25-MAR- 99 .. G: %: M e d i a n value of the highest individual daily grades P e k e n t of affected animals (0 = less than 5%. 1 = betwen 5% and IS%,.,., 58 A = more than 9 5 % ) BACK TO MAIN NOTOX Project 2 4 2 9 3 3 - SYM-SUM 23 17-MA3.- 99 CLINICAI; SIGNS ( m y ) FEMALES GROUP 6 (PFOS) SIGN (MAX.GRADE1 TFEmMEm WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: RED S T A I N I x (3) (NECK) G: %: ...........................,............;...............................1111111111111111............................ G: %: ............... ...............1111111111111111111111111111...111111111111 11111lllllllllllllllllllllll...llllllllllll 0: %: ............................................................ 111111112..222222222222222.. 111111111..111111111111111.. G: %: ............................................................. 222222222..222222222222222.. lllllllll..lllllllllllllll.. G: %: .......................................................................... 11111111111111............................ G: %: .................................................................................................................... G: %: .................................................................................................................... G: 5: .................................................................................................................... G: %: ...................................................................................................... .. llll... 1111. G: 8: ........................................................................................llllllll.................... G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 Ibetween 5% and IS,..., T h e after treatment (H.Mi) A: 0 . 0 0 B: 4.00 59 A = mre than 95%) NOTOX Proiect 242933 CLINICAL SIGNS, DAILY (.SUMMARY) FEMALES GROUP 6 (PFOS) SIGN (MAx.GRADE) LCCATION -s.K..I.N../.F.u.R../.P.L..r.J.M..A.GE RED (3) (HEAD) RED -AINms (3) (EAR LEFT) RECOVERY WEEKS: 1......2......3......4......5. G: %: 1121212121............................................... G: %: i1i2i2i5i5i3i3i2i2i2i2l2i3l3............................ G: %: ......121212121212121212................................ G: %: ..................122121................................ G: %: ..................1221212121............i............... BACK TO MAIN SYM-SUM - 24 2 5-MAR- 9 9 G: %: Median value of the highest individual daily grades Pelrcent of affected animals (0 = less than 5%, 1 = between 5% and 15%, .... A = more than 95%) 60 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS BACK TO MAIN BW0-9S-UDMEC- - 981 BODY WEIGHTS (GRAM) S U M M A R Y MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS DAY 1 MEAN 208 212 209 210 208 210 WEEK 1 ST.DEV. 14.0 13.5 9.2 9.4 13.8 10.9 N 14 14 14 14 14 14 DAY 5 MEAN 232 237 234 WEEK 1 ST.DN. 14.9 14.4 8.9 N 14 14 14 238 . 231 10.8 16.5 14 14 237 13.9 14 DAY 8 MEAN 248 252 248 252 246 25 2 WEEK 2 ST.DEV. 19.8 17.6 11.8 13.2 23.4 17.8 N 14 14 14 14 14 14 DAY 12 WEEK 2 MEAN ST.DEV. N 272 277 273 20.7 18.6 9.3 14 14 14 279 270 279 17.7 24.5 21 .o 14 14 14 DAY 15 MEAN 281 288 283 288 275 288 WEEK 3 ST.DEV. 25.2 22.9 10.9 19.4 26.3 25.2 N 14 14 14 14 14 14 DAY 19 MEAN 302 308 304 312 301 310 WEEK 3 ST.DV. 26.8 23.2 10.3 24.1 30.4 29.0 N 14 14 14 14 14 14 DAY 22 MEAN 312 316 315 WEEK 4 ST.DN. 27.4 23.6 9.5 N 14 14 14 319 26.5 14 307 31.9 14 318 31.4 14 DAY 25 MEAN 324 329 328 333 321 333 WEEK 4 , ST.DEV. 29.8 25.4 12.6 28.4 34.8 33.5 N 14 14 14 14 14 14 DAY 28 MEAN 336 340 338 347 328 342 I WEEK 4 ST.DN. 29.9 27.5 13.0 31.6' 37.1 33.7 N 14 14 14 14 14 14 I * * : D u n n e t t - T e s t based on pooled v a r i a n c e s i g n i f i c a n t a t 5% (') o r 1%(") l e v e l 61 NOTOX P r o j e c t 242933 TELOMER ALCOHOL lTELOMER ACRYLATE/ .. PFHSI PFOS- BACK TO MAIN - BW-SUM 2 09-DEC- 98 BODY WEIGHTS (GRAM) SUMMARY MALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE' GROUP 4 GROUP 5 PFHS DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 14 WEEK 2 DAY 19 WEEK 3 DAY 22 WEEK 4 *DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 304 27.9 6 338 31.2 6 346 32.8 6 358 32.8 6 362 32.4 6 391 24.6 3 399 24.2 3 412 25.2 3 415 26.7 3 298 20.2 6 314 12.4 6 331 351 28.8 23.5 6 6 338 28.1 6 356 20.9 6 350 ' 372 28.5 21.0 6 6 35 1 28.6 6 374 20.8 6 375 389 35.7 7.2 3 3 382 399 33.5 5.5 3 3 391 407 38.5 7.8 3 3 39 1 412 37.1 2.6 3 3 330 28.2 6 372 31.8 6 383 35.2 6 400 36.9 6 398 38.0 6 419 35.0 3 426 37.6 3 437 38.7 3 435 37.6 3 29 1 40.4 6 327 45.4 6 338 46.9 6 344 48.5 -6 345 47.6 6 347 19.1 3 351 19.1 3 368 20.8 3 362 20.8 3 GROUP 6 PFOS 313 40.4 6 352 46.8 6 360 46.0 6 374 46.8 6 376 47.6 6 351 39.7 3 359 38.6 3 371 34.8 3 370 39.3 3 ' I * * : Dunnett-Test based on pooled varlance significant at 5% ( * ) or 1%(") level .I 62 NOTOX P r o j e c t 242933 TELOMER ALCOHOLlTELOMER ACRYLATE]. BACK TO MAIN - BW-SUM 3 - 09-DEC 98 BODY WEIGHTS~GRAM)SUMMARY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 15 WEEK 3 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK' 4 DAY 28 ' WEEK 4 MEAN ST.DEV. .N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DW. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 174 11.9 14 181 . 14.3 14 188 15.6 14 202 16.2 14 206 19.8 14 219 20. I 14 221 19.4 14 232 21.4 14 236 21.8 14 169 8.0 14 176 8.5 14 '182 11.9 14 194 11.9 14 200 15.0 14 213 15.5 14 216 15.7 14 227 16.5 14 230 16.9 14 166 10.0 14 173. . 11.2 14 179 13.2 14 191 13.3 14 192 15.0 14 203 15.5 14 207 16.9 14 218 17.3 14 219 17.0 14 170 11.9 14 177 14.5 14 182 15.9 14 198 16.8 14 202 18.2 14 214 19.6 14 218 20.4 14 230 18.6 14 231 20.5 14 169 8.0 14 174 10.4 14 180 9.0 14 194 10.9 14 196 11.3 14 209 11.3 14 212 12.6 14 222 13.0 14 224 12.6 14. ~~ / * * : Dunnett-Test based on pooled variance significant at 5% ( * ) or 1%(") level GROUP 6 PFOS 166 8.7 14 171 9.8 14 176 10.1 14 188 ' 9.8 14 187 11.5 14 198 * * 12.7 14 200 ' * 13.2 14 207 * * 14.3 14 207 * * 13.3 14 63 NOTOX P r o j e c t 242933 TELOMER ALCOHOL lTELOMER ACRYLATE/ PFHS /bFOS- BACK TO MAIN BW-SUM - 4 09-DEC-98 BODY WEIGHTS (GRAM) SUMMARY FEMALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS DAY 1 MEAN 213 WEEK 1 ST.DEV. . 21.9 N. 6 .. .' DAY 5 . MEAN 239 WEEK 1 ST.DN. 24.1 N 6 DAY 8 WEEK 2 MEAN ST.DEV. N 244 25.1 6 f DAY 12 MEAN 251 WEEK 2 ST.DEV. 25.8 N 6 DAY 14 MEAN 257 . WEEK 2 ST.DEV. 27.3 N 6 DAY 19 MEAN 254 WEEK 3 ST.DEV. 27.5 N 3 DAY 22 MEAN 258 WEEK 4 ST.DEV. 28.0 N 3 DAY 25 MEAN 263 WEEK 4 ST.DEV. 28.6 N 3 DAY 28 MEAN 268 WEEK 4 ST.DEV. 27.8 N 3 200 9.9 6 226 11.3 6 229 12.7 6 238 12.3 6 237 16.7 6 246 13.2 3 25 1 12.9 3 251 10.8 3 251 12.7 3 193 16.2 6 219 18.2 6 222 19.9 6 233 20.2 6 231 20.6 6 245 26.6 3 248 25.3 3 249 25.4 3 248 26.0 3 212 23.9 6 236 20.3 6 240 21.1 6 248 23.0 6 248 20.0 6 253 29.1 3 258 30.3 3 265 31.0 3 .262 35.5 3 206 185 12.8 7.0 6 6 231 207 14.4 9.5 6 6 235 213 14.1 8.6 6 6 243 219 12.8 8.2 6 6 241 13.6 6 220 .* - 5.5 6 243 12.9 3 226 11.3 3 246 . 224 14.5 9.8 3 3 254 17.3 3 226 10.7 3 254 13.2 3 229 13.0 3 / * * : Dunnett-Test based on pooled variance significant at 5% ( * ) or 1% (") level I 64 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - PFHS/PFOS BACK TO MAIN BWG-SUM - 1 09-DEC-98 BODY WEIGHT GAIN ('Yo) SUMMARY MALES TREATMENT GROUP 1 VEHICLE CONTROL DAY 1 WEEK 1 DAY 5 WEEK 1 DAY a WEEK 2 DAY 12 WEEK 2 DAY 15 WEEK 3 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N , MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 0 0.0 14 12 6.3 14 19 7.4 14 si 7.9 14 35 9.5 14 . 45 10.1 14 50 10.4 14 56 11.2 14 61 11.4 14 GROUP 2 TELOMER ALCOHOL 0 0.0 14 12 1.9 14 19 3.3 14 30 4.8 14 36 6.5 14 45 7.6 14 49 7.9 14 55 8.8 14 60 9.1 14 GROUP 3 TELOMER ACRYLATE GROUP 4 0 0 0.0 0.0 14 14 12 13 1.5 2.3 14 14 19 20 . 3.7 3.5 14 14 31 33 4.7 5.5 14 14 35 37 5.0 6.5 14 14 45 4a 5.8 9.0 14 14 51 52 5.7 9.9 14 14 57 58 6.5 11.4 14 14 62 65 7.1 12.9 14 14 GROUP 5 PFHS . GROUP 6 PFOS 0 0 0.0 0.0 14 14 11 13 2.2 ' 1.5 14 14 ia 20 4.6 4.2 14 14 30 33 4.5 4.a 14 14 32 37 7.2 7.4 14 14 45 47 6.7 8.9 14 14 48 51 7.3 10.3 14 14 54 58 8.5 10.8 14 14 58 62 9.6 11.1 14 14 I .* : Dunnett-Test basad on pooled variance significant at 5% ( * ) or 1% ( " ) level NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS I PFOS BACK TO MAIN BWG-SUM - 2 09-DEC-98 BODY WEIGHT GAIN ("h)SUMMARY MALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS DAY 1 MEAN 51 WEEK 1 ST.DEV. 7.9 N 6 DAY 5 MEAN 68 WEEK 1 ST.DEV. 9.7 N 6 DAY 8 MEAN 72 WEEK 2 ST.DEV. 9.8 N 6 DAY 12 MEAN 78 WEEK 2 ST.DEV. 9.1 N 6 DAY 14 MEAN 80 WEEK 2 ST.DEV. 9.0 N 6 DAY 19 MEAN 92 WEEK 3 ST.DEV. 6.4 N 3 DAY 22 MEAN 97 WEEK 4 ST.DEV. 5.7 N 3 DAY 25 MEAN 103 WEEK 4 ST.DEV. 6.2 N 3 DAY 28 MEAN 105 WEEK 4 ST.DEV. 6.5 N 3 45 51 5.8 3.8 6 6 61 69 8.7 7.4 6 6 65 72 8.6 6.8 6 6 71 79 8.2 7.3 6 6 71 80 8.9 7.0 6 6 77 88 13.0 7.1 3 3 80 93 12.0 6.3 3 3 84 97 14.2 6.9 3 3 84 99 13.5 6.1 3 3 57 10.6 6 78 12.3 6 83 12.8 6 91 14.1 6 90 15.8 6 93 5.8 3' 97 6.8 3 102 7.3 3 101 7.6 3 45 12.6 . 6 63 13.9 6 69 14.4 6 72 15.6 6 72 15.2 6 77 7.1 3 79 7.2 3 87 9.9 3 84 8.1 3 ~ I * * : Dunnett-Test based on pooled varlance significant at 5% ( * I or 1% (") level GROUP 6 PFOS 49 14.5 6 68 16.5 6 71 16.5 6 78 16.6 6 79 17.2 6 70 5.6 3 74 4.9 3 80 * 2.7 3 80 4.5 3 66 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. PFHSlPFOS BACK TO MAIN - BWG-SUM 3 0 -9DEC9- 8 BODY WEIGHT GAIN ("7) SUMMARY FEMALES. TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 DAY 1 MEAN 0 0 0 0 WEEK 1 ST. OW. 0.0 0.0 0.0 0.0 N 14 14 -. 14 14 DAY 5 MEAN 4 5 4 4 WEEK 1 ST.DEV. 2.7 2.5 2.3 2.3 N 14 14 14 14 - DAY 8 MEAN 8 .8 a 7 WEEK 2 ST. DEV, 3.2 3.9 2.6 3.2 N 14 14 14 14 DAY 12 MEAN 16 15 15 17 . WEEK 2 ST.DEV. 4.3 4.2 2.9 3.5 N 14 14 14 14 DAY 15 MEAN 18 18 16 19 WEEK 3 ST.DEV. 5.5 5.8 4.3 4.2 N 14 14 14 14 DAY 19 MEAN 26 26 1 23 26 WEEK 3 ST.DEV. 5.6 5.8 5.3 4.2 N 14 14 14 14 DAY 22 MEAN 27 28 25 28 WEEK 4 ST. DEV. 4.5 5.5 6.2 4.6 N 14 14 14 14 DAY 25 MEAN 33 34 31 35 WEEK 4 ST.DEV. 6.7 5.8 5.6 3.9 N 14 14 14 14 DAY 2a MEAN 36 36 32 36 WEEK 4 ST.DEV. 6.7 7.0 4.8 4.5 N 14 14 14 14 GROUP 5 PFHS 0 0.0 14 3 3.2 14 7 2.9 14 14 3.4 14 16 3.7 14 23 3.9 14 26 4.5 14 31 4.3 14 33 4.9 14 GROUP 6 PFOS 0 0.0 14 . 3 3.4 14 6 2.7 14 13 4.0 14 13 4.6 14 19 6.4 14 21 6.9 14 24 * * 7.3 14 25 * * 7.5 14 I ' . : Dunnett-Test based on pooled variance significant at 5% ( * ) o r 1% (") level 67 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. PFHS/ PFOS BACK TO MAIN BWG-SUM - 4 0 9 - D E C -98 BODY WEIGHT GAIN (%) SUMMARY FEMALES RECOVERY GROUP 1 VEHICLE CONTROL DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 14 WEEK 2 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 23 5.7 6 37 6.4 6 41 7.0 6 44 7.2 6 48 8.8 6 47 12.5 3 50 12.1 3 53 12.7 3 56 13.8 3 GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE I GROUP 4 21 18 25 3.7 7.7 4.4 6 .6 6 37 35 39 4.6 9.5 3.7 6 6 6 39 36 41 5.4 9.9 '5.7 6 6 6 44 43 46 4.8 9.8 6.1 6 '6 6 43 42 46 7.8 9.8 4.6 6 6 6 50 48 45 7.0 4.8 5.1 3 3 3 53 50 48 6.7 6.2 3.7 3 3 3 53 50 52 5.4 3.8 2.8 3 3 3 53 50 50 6.2 3.0 5.6 3 3 3 GROUP 5 PFHS 22 5.0 6 37 6.1 6 39 6.2 6 44 7.2 6 43 8.56 45 8.6 3 47 9.5 3 52 7.6 3 52 8.8 3 GROUP 6 PFOS 15 6.1 6 29 7.7. 6 33 7.3 6 37 7.2 6 37 5.9 6 41 10.3 3 39 9.5 3 40 10.0 3 42 11.5 3 I *' : Dunnett-Test based on pooled variance significant at 5% (') or 1% (") level 68 NOTOX Project 242933 TELOMER ALCOHOL lTELOMER ACRYLATE/ PFHS lbFOS& BACK TO MAIN FC-SUM - 1 01-MAR- 99 FOOD CONSUMPTION (WANIMAUDAY) SUMMARY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 ' GROUP 3 TELOMER TELOMER ALCOHOL ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS DAYS 1 - 8 MEAN 26 26 27 26 26 27 WEEKS 112 ST.DEV. 2.1 1.9 1.0 1.0 2.4 0.9 N (CAGE) 4 4 4 4 4 4 DAYS 8-15 MEAN 28 28 28 29 27 29 WEEKS 213 ST.DEV. 2.7 2.4 0.9 0.6 2.1 1.6 N (CAGE) 4 4 4 4 4 4 DAYS 15-22 MEAN 30 ' 29 30 30 29 30 WEEKS 314 ST.DEV. 3.3 1.3 1.5 2.3 1.2 2.7 N (CAGE) 4 4 4 4 4 4 i DAYS 22-28 MEAN 30 30 31 . 31 30 31 WEEK 4 ST.DEV. 2.3 1.5 1.1 2.4 1.6 3.0 N (CAGE) 4' 4 4 4 4 4 I MEAN OF MEANS OVER TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS MEAN 28 28 29 29 28 29 ' I * * : Dunnett-Test based on pooled variance s i g n i f i c a n t a t 5% (') o r 1%(") l e v e l 69 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - P F H S /PFOS BACK TO MAIN FC-SUM - 2 01-MAR - 99 FOOD CONSUMPTION (GIANIMAUQAY)SUMMARY MALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 DAYS 1-8 MEAN 30 28 30 32 WEEKS 112 ST.DN. 2.5 2.0 1.8 1.4 N (CAGE) 2 2 2 2 DAYS 8-14 MEAN 30 28 31 31 . WEEK 2 ST.DEV. 1.8 1.8 1.4 2.0 N (CAGE) 2 2 2 2 DAYS 14-19 WEEKS 213 MEAN ST.DEV. 3.0.. 2.8.. 3.0.. 3-0.. N (CAGE) 1 1 1 1 ... ... ... DAYS 19-22 MEAN 31 29 32 31 WEEKS 314 ST.DEV. .I_ N (CAGE) 1 1 1 1 ... -.. ..- DAYS 22-28 MEAN 31 28 30 29 WEEK 4 ST.DEV. . I N (CAGE) 1 1 1 1 GROUP 5 PFHS 30 0.8 2 29 1.2 2 2.6.. 1 27 -e. 1 27 e-. 1 GROUP 6 PFOS 30 3.7 2 30 4.0 2 27 e.. 1 28 *-. 1 27 1-. 1 MEAN OF MEANS OVER RECOVERY MEAN GROUP 1 VEHICLE CONTROL 30 GROUP 2 TELOMER ALCOHOL 28 GROUP 3 TELOMER ACRYLATE 30 GROUP 4 ~ 30 GROUP 5 PFHS 28 GROUP 6 PFOS ~ 28 I * * : Dunnett-Test based on pooled variance s i g n i f i c a n t a t 5% ( * ) or 1%(") l e v e l 70 NOTOX Project 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ .. PFHS IPFOS- BACK TO MAIN FC-SUM - 3 01-MAR * 99 FOOD CONSUMPTION (WANIMAUDAY) SUMMARY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 DAYS 1-8 WEEKS 112 DAYS 8-15 WEEKS 213 DAYS 15-22 WEEKS 314 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) 19 18 0.7 1.2 4 '4 21 20 0.5 0.5 4 4 22 22 1.3 0.8 4 4 24 23 1.2 0.7 4 4 19 19 1.2 0.6 4 4 20 21 1.1 0.8 4 4 22 22 1.2 1.1 4 4 23 23 1.3 0.5 4 4 GROUP 5 PFHS 20 1.6 4 21 1.4 4 22 1.3 4 23 0.7 4 GROUP 6 PFOS 19 1.9 4 19 0.8 4 20 1.0 4 20 * * 0.5 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 22 GROUP 2 TELOMER ALCOHOL 21 GROUP 3 TELOMER ACRYLATE GROUP 4 21 21 GROUP 5 PFHS 22 GROUP 6 PFOS 19 ~ ~~ I * * : Dunnett-Test based on pooled variance significant at 5% ( * ) or 1%(") level 71 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. PFHSIPFOS- FOOD CONSUMPTION(WANIMAUDAY) SUMMARY FEMALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 . DAYS 1.8 MEAN 25 24 WEEKS 112 ST. DEV 0.4 1.0 N (CAGE) 2 2 DAYS 8-14 MEAN 24 23 WEEK 2 ST.DEV. 0.2 1.1 N (CAGE) 2 2 . ... ... DAYS 14-19 MEAN 22 22 WEEKS 213 ST. DEV N (CAGE) 1 1 DAYS 19-22 WEEKS 314 MEAN ST.DEV. 2.4.. 2.3.. N (CAGE) 1 1 DAYS 22-28 WEEK 4 MEAN ST.DEV. 2.4-. 22 N (CAGE) 1 1 24 1.2 2 23 0.8 2 2-2.. 1 22 1 22 .e. 1. 24 1.1 2 23 '1. 2 2 2.0.- 1 21 e. 1 22 I. 1 BACK TO MAIN FC-SUM - 4 01 -MAR - 99 GROUP 5 PFHS GROUP 6 PFOS 24 0.6 2 24 0.6 2 2-0.- 1 22 1 21 1 21 * * 0.2 2 20 0.3 2 1.9.. 1 1.9.- 1 1-9.- 1 MEAN OF MEANS OVER RECOVERY MEAN GROUP 1 VEHICLE CONTROL 24 GROUP 2 TELOMER ALCOHOL 23 GROUP 3 TELOMER ACRYLATE GROUP 4 22 22 GROUP 5 PFHS 22 GROUP 6 PFOS 20 / * * : Dunnett-Test based on pooled variance significant at 5% ( * ) or 1% (") level 72 NOTOX P r o j e c t 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ .. PFHSIPFOS- - BACK TO MAIN - RFC-SUM 1 01 -MAR 99 RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL DAYS 1-8 MEAN 114 WEEKS 112 ST.DEV. 8.7 N (CAGE) 4 DAYS 8-15 MEAN 103 WEEKS 213 ST.DEV. 9.3 N (CAGE) 4 QAYS 15-22 MEAN 99 WEEKS 314 ST.DEV. 11.1 N (CAGE) 4 DAYS 22-28 MEAN 93 WEEK 4 ST.DEV. 6.8 N (CAGE) 4 112 3.6 4 . 102 3.3 4 96 ' 4.5 4 91 1.1 4 GROUP 3 TELOMER ACRYLATE 115 3.8 4 103 2.0 4 99 5.2 4 94 3.4 4 GROUP 4 ill 2.9 4 102 2.3 4 96 5.9 4 92 5.3 4 GROUP 5 PFHS 112 7.1 4 101 2.6 4 98 5.5 4 93 3.6 4 GROUP 6 PFOS 115 3.7 4 '104 1.9 4 98 6.8 4 94 6.1 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 102 GROUP 2 TELOMER ALCOHOL 100 GROUP 3 TELOMER ACRYLATE GROUP 4 103 100 GROUP 5 PFHS ' GROUP 6 PFOS 101 103 / * * : Dunnett-Test based on pooled variance s i g n i f i c a n t a t 5% (') or 1%( " ) level 73 NOTOX P r o j e c t 242933 TELOMER ALCOHOLlTELOMER ACRYLATE/ ._ PFHSIPFOS- RELATIVE FOOD CONSUMPTIONSUMMARY (G/KG BODY WEIGHTDAY) MALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL DAYS 1 - 8 MEAN 90 WEEKS 112 ST.DEV. 1.5 N (CAGE) 2 DAYS 8-14 MEAN 84 WEEK 2 ST.DN. 0.1 N (CAGE) 2 DAYS 14-19 MEAN 77 WEEKS 213 ST.DEV. 1-1 N (CAGE) 1 DAYS 19-22 WEEKS 314 MEAN ST.DN. 7.8.. N .(CAGE) 1 DAYS 22-28 MEAN 75 WEEK 4 ST.DEV. I-. N (CAGE) 1 85 0.9 2 . 80 . 0.7 2 7.4.. 1 7.6.. 1 7-2-. 1 GROUP 3 TELOMER ACRYLATE 87 4.9 2 82 2.6 2 7.6.. 1 8.0.. 1 7.4- - 1 GROUP 4 85 4.1 2 78 5.8 , 2 7.1.. 1 7.2.. 1 6.7.- 1 BACK TO MAIN RFC-SUM - 2 01-MAR- 99 GROUP 5 PFHS GROUP 6 PFOS 91 86 0.3 2.1 2 2 83 81 0.1 0.2 2 2 - - - 76 76 ..I 1 1 MEAN OF MEANS OVER RECOVERY * MEAN GROUP 1 VEHICLE CONTROL 81 GROUP 2 TELOMER ALCOHOL n GROUP 3 TELOMER ACRYLATE ao GROUP 4 74 GROUP 5 PFHS ao GROUP 6 PFOS 79 / : D u n n e t t - T e s t based on pooled v a r i a n c e s i g n i f i c a n t a t 5% ( * ) o r 1%(") l e v e l 74 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHSiP F O ~ BACK TO MAIN - RFC-SUM 3 - 01- MAR 99 RELATIVE FOOD CONSUMPTION SUMMARY (WKG BODY WEIGHTDAY) FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL DAYS 1 - 8 MEAN 106 104 WEEKS 112 ST.DEV. 1.2 6.1 N (CAGE) 4 4 DAYS 8-15 MEAN 104 105 WEEKS 213 ST.DEV. 2.4 2.8 N (CAGE) 4 4 . DAYS 15-22 MEAN 103 105 WEEKS 314 ST. DEV 6.8 6.7 N (CAW 4 4 DAYS 22.28 MEAN 102 102 b WEEK 4 ST.DEV. 6.1 4.6 N (CAGE) 4 4 GROUP 3 TELOMER ACRYLATE 110 5.0 4 107 4.7 4 108 5.7 4 105 2.8 4 GROUP 4 108 5.4 4 107 4.7 4 105 6.2 4 101 3.4 4 GROUP 5 PFHS 113 11.8 4 109 8.0 4 107 4.9 4 104 1.9 4 GROUP 6 PFOS- 111 11.4 4 103 6.4 4 100 7.9 4 95 4.6 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 104 GROUP 2 TELOMER ALCOHOL 104 GROUP 3 TELOMER ACRYLATE 107 GROUP 4 105 GROUP 5 PFHS 108 GROUP 6 PFOS 102 . .. ~ -~ I * * : Dunnett-Test based on pooled variance significant at 5% ( * ) or 1%(") level 75 NOTOX P r oSect 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/, - .. PFHSIPFOS BACK TO MAIN - RFC-SUM 4 01-MAR-99 RELATIVE FOOD CONSUMPTION SUMMARY (WKG BODY WEIGHT/DAY) FEMALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS DAYS 1-8 MEAN . WEEKS 112 ST.DEV. N (CAGE) DAYS 8-14 WEEK 2 MEAN ST.DEV. N (CAGE) DAYS 14-19 WEWS 213 MEhN ST.DEV. N (CAGE) . . DAYS 19-22 MEAN WEEKS 314 ST.DEV. N (CAGE) . DAYS 22-28 MEAN WEEK 4 ST. DEV ! N (CAGE) 105 3.5 2 95 0.2 2 8.6.- 1 9.2.. 1 9.1.- 1 104 107 102 106 100 2.7 2.4 5.3 1.2 4.0 2 ' 2 2 2 2 95 97 94 97 93 3.7 1.0 4.0 0.2 3.9 2 2 2 2 2 88 88 I-- I-. 1 1 8.1.- 84 1 1 85 I-. 1 .-- --. .-- 92 87 83 88 86 I.. . e 1 1 1 1 1 .-. ... -.- 86 90 81 . 84 85 I.. a.. 1 1 1 1 1 MEAN OF MEANS OVER RECOVERY MEAN GROUP 1 VEHICLE CONTROL 94 GROUP 2 TELOMER ALCOHOL 93 ' GROUP 3 TELOMER ACRYLATE 94 GROUP 4 88 GROUP 5 PFHS 92 GROUP 6 PFOS 90 / '* : Dunnett-Test based on pooled variance significant at 5% ( * ) or 1%(") level 76 NOTOX P r o j e c t 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ PFHS/ PFOS BACK TO MAIN - HEM-SUM 1 09-NOV-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS MALES TREATMENT HAEMATOLOGY PARAMETERS -_I.-....._.._..__..-. RBC TI1 HB moll1 HCT 111 MCV fl MCH fmol MCHC 'mmolll RDW x PLATELETS GI 1 WBC GI 1 SEG . I EO. 1 BAS0 . 1 MONO. 1 LYMPH. 1 MEAN ST.DEV. N MEAN ST.DN. N MW ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DN. N MEAN' ST.DEV. N MEAN ST.DEV. N MEAN ST:DEV. N MEAN ST.DN. N GROUP 1 VEHICLE CONTROL 7.05 0.24 10 9.1 0.4 10 0.407 0.011 10 57.8 1.1 10 1.294 0.036 10 22.4 0.4 10 12.4 0.4 10 1182 110 10 11.3 3.5 10 0.124 0.033 10 0,007 0.005 10 0.000 0.000 10 0.026 0.006 10 0.844 0.030 10 GROUP 2 TELOMER ALCOHOL 6.72 0.32 14 8.8 0.4 14 0.395 0.016 14 58.8 1.4 14 1.313 0.030 14 22.3 0.4 14 12.0 0.7 14 1025 116 14 10.0 2.0 14 0.125 0.041 14 0.011 0.011 14 0.000 0.000 14 0.022 0.012 14 0.841 0.048 14 GROUP 3 TELOMER ACRYLATE GROUP 4 6.61 0.27 14 8.7 0.4 14 0.388 0.014 14 58.7 1.0 14 1.317 0.027 14 22.4 0.5 14 12.2 0.6 14 1115 158 14 10.2 2.1 14 0.117 0.035 14 0.007 0.005 14 0 a 000 0.000 14 0.020 0.010 14 0.857 0.040 14 6.79 0.32 13 8.8 0.5 13 0.397 0.021 13 58.5 1.2 13 1.297 0.045 13 22.2 0.4 ' 13 12.0 0.3 13 1050 158 13 11.7 2.4 13 0.087 + 0.025 13 0.007 0.004 . 13 0.000 0.000 13 0.023 0.009 13 0.883 + 0.031 13 GROUP 5 PFHS 6.57 ' 0.52 14 8.5 *' 0.6 14 0.383 0.026 14 58.4 ' 1.8 14 1:297 0.035 14 22.2 0.4 14 12.0 0.7 14 870 * * 306 14 9.7 3.0 14 0.148 0.049 14 0.008 0.007 14 u.000 0.000 14 0.026 0.013 14 0.818 0.053 14 GROUP 6 PFOS 6.66 0.39 12 8.9 0.4 12 0.392 0.019 12 59.0 1.9 12 1.338 * 0.047 12 22.7 0.4 12 12.0 0.a 12 1092 75 12 10.7 2.2 12 0.114 0.039 12 0.005 0.005 12 0.000 0.000 12 0.023 0.009 12 0.858 0.041 12 *I**Du:nnett-test based on pooled variance sig. at 5% or 1%level. +: Steel-test s i g at 5% level. 77 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/' .. PFHSlPFOS HAEMATOLOGY SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL HAEMATOLOGY PARAMETERS .._._......_.__....I.. PT MEAN 14.9 SEC ST.DEV. 0.6 N 13 PTT MEAN 16.1 SEC ST.DEV. 0.8 N 13 GROUP 2 TELOMER ALCOHOL 15.0 0.6 14 16.0 0.7 14 GROUP 3 TELOMER' ACRYLATE GROUP 4 14.9 0.6 13 17.1 2.2 13 14.4 0.6 12 18.2 3.2 11 BACK TO MAIN - HEM-SUM 2 09-NOV-98 GROUP 5 PFHS GROUP 6 PFOS 14.1 ** 0.4 9 16.8 0.8 9 14.7 0.6 13 17.2 1.8 13 * I . . : Dunnett-test based on pooled variance s i g . at 5% or 1% level. +: Steel-test s i g . at 5% level. 78 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - .. PFHS/PFOS BACK TO MAIN - HEM-SUM 3 -09 NOV-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS HAEMATOLOGY PARAMETERS RBC MEAN 6.72 6.27 *' 6.61 6.62 6.35 6.41 TI 1 ST.DEV. 0.14 0.39 0.34 0.36 0.28 0.37 N 11 13 13 13 13 11 HB MEAN 9.1 8.5 8.8 9.0 8.7 8.6 moll1 ST.DEV. 0.3 0.5 0.5 0.4 0.4 0.5 N 11 13 13 13 13 11 HCT MEAN 0.378 0.355 *' 0.370 0.377 0.358 0.359' 111 ST.DN. 0.012 0.018 0.019 0.014 0.017 0.019 N 11 13 13 13 13 11 MCV f1 MCH fmol MEAN ST.DEV. N MEAN ST.DEV. N 56.2 1.9 11 1.350 0.034 11 56.6 1.4 13 1.356 0.035 13 56.0 1.7 13 1.329 0.049 13 57.1 1.9 13 1.363 0.053 13 56.3 2.6 14 1.356 0.050 13 56.0 1.9 11 1.348 0.041 11 KHC MEAN 24.0 24.0 23.7 23.9 24.3 24.1 moll1 ST.DEV. 0.5 0.6 0.6 0.5 0.5 0.2 N 11 13 13 13 13 11 ROW MEAN 11.3 11.2 11.4 11.3 11.3' 11.2 % ST.DEV. 0.4 0.5 0.6 0.3 0.6 0.5 N. 11 13 13 13 13 11 PLATELETS MEAN 1166 1141 1149 1169 1035 1134 GI 1 ST.DEV. 127 149 125 74 236 131 N 11 13 13 13 13 11 WBC GI 1 SEG . 1 MEAN ST.DEV. N MEAN ST.DEV. N 7.4 2.1 11 0.082 0.027 11 7.5 1.6 13 0.103 0.041 13 6.8 2.3 13 0.109 0.039 13 7.6 1.8 13 0.087 0.024 13 6.3 1.9 13 0.080 ' 0.035 13 8.4 2.4 11 0.077 0.020 11 EO. 1 BAS0 . 1 MEAN ST.DEV. N . MEAN ST. DEV N 0.010 0.007 11 0.000 0.000 11 0.004 0.005 13 0.000 0.000 13 0.009 0.009 13 0 * 000 0.000 13 0.011 0.006 13 0.000 0.000 13 0.009 0.006 13 0.000 0.000 13 0.005 0.004 11 0.000 0.000 11 MONO. 1 MEAN ST.DEV . N 0.025 0.011 11 0.020 0.011 13 0.017 0.009 13 0.024 0.008 13 0 .a24 0.012 13 0.020 0.007 11 L W H. 1 MEAN ST.DEV. 0.883 0.034 0.873 0.044 0.865 0.040 0.877 0.030 0.887 0.039 0.898 0.026 N 11 13 13 13 13 11 '/*': Dunnett-test based on pooled variance sig. at 5% or 1%level. t: Steel-test sig. at 5% level 79 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. c PFHS/PFOS HAEMATOLOGY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT HAEMATOLOGY PARAMETERS . . . I . - . . . . . . . . - . . . . . . - PT MEAN SEC ST.DEY. N PTT MEAN SEC 3T.DEV. N GROUP 1 VEHICLE CONTROL 15.4 1.0 14 15.9 1.4 14 GROUP 2 TELOMER ALCOHOL 15.0 0.6 14 15.0 0.9 14 GROUP 3 TELOMER ACRYLATE GROUP 4 15.0 0.8 13 15.7 0.8 12 14.5 0.5 13 17.0 0.9 13 BACK TO MAIN HEM-SUM - 4 09-NOV-98 GROUP 5 PFHS GROUP 6 PFOS 14.5 0.7 12 16.8 2.0 11 ' 14.4 *I 0.4 14 16.5 2.2 14 ' 1 " : Dunnett-test based on pooled variance s i g . at 5% or 1%level. t: Steel-test s l g . at 5% level. 80 NOTOX P r o j e c t 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ .. d PFHS /PFOS BACK TO MAIN HEM-SUM - S 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS H..A.E.M..A.T..O.L.O..G.Y...P.A..R.A.METERS RBC MEAN 7.23 7.04 6.97 7.00 6.97 7.01 T I1 ST.DEV. 0.18 0.25 0.27 0.27 0.21 0.22 N 3 3 3 3 3 3 HE MEAN 9.1 8.9 8.7 8.8 8.6 8.9 moll1 ST.DEV. 0.4 0.1 0.5 0.1 0.1 0.0 N 3 3 3 3 3 3 . HCT MEAN 0.417 0.404 ' 0.401 0.402 0.392 0.408 111 ST. DEV 0.011 0.008 0.018 0.014 0.004 0.006 N 3 3 3 3 3 3 MCV MEAN 57.7 57.3 57.5 57.4 56.3 58.2 f1 ST.DEV. 0.1 1.0 0.6 0.3 1.6 1.0 N 3 3 3 3 3 3 MCH fmol MEAN ST.DEV. N 1.259 0.036 3 1.260 0.037 3 1.253 0.028 3 1.259 0.034 3 1.230 0.020 3 1.270 0.041 3 MCHC MEAN 21.8 22.0 21.8 21.9 21.8 21.8 mol / 1 ST.DN. 0.6 0.3 0.3 0.5 0.3 0.3 N 3 3 3 3 3 3 ROW MEAN 14.1 13.7 13.4 13.3 14.4 , 13.1 % ST.DEV. 0.5 0.6 0.8 1.3 0.5 0.8 N 3 3 3 3 3 3 PLATELETS GI1 MEAN ST.DEV, N 1177 106 3 1081 102 3 1186 1080 170 49 3 '3 1053 22 3 1078 58 3' WBC MEAN 8.5 12.0 12.1 ' 12.2 8.5 1.1.6 GI 1 ST.DEV. 1.8 3.2 1.9 1.8 2.4 1.9 N 3 3 3 3 3 3 SEG. MEAN 0.112 0.127 0.088 0.058 0,133 0.137 1 ST.DEV. 0.043 0.073 0.048 0.019 0.063 0.016 N 3 '3 3 3 3 3. EO. 1 BAS0 . 1 MEAN ST.DEV. N MEAN ST.DEV. N 0.013 0.003 3 0.000 0.000 3 0.005 0.005 3 0.000 0.000 3 0.005 0.005 3 0.000 0.000 3 0.003 0.003 3 0.000 0.000 3 0.012 0.003 3 0.000 0.000 3 0.002 0.003 3 0.000 0.000 3 MONO. 1 MEAN ST.DEV. N 0.027 0.003 3 0.022 0.008 3 0.025 0.005 3 0.023 0.006 3 0.018 0.008 3 0.018 0.003 3 LYMPH. MEAN 0.848 0.847 0.882 0.915 0.837 0.843 1 ST.DEV. 0.043 0.077 0.055 0.022 0.073 0.020 N 3 3 3 3 3 3 ' I . ' : Dunnett-test based on pooled variance s i g . at 5% o r 1%level. +: Steel test s i g . at 5% level. 81 NOTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ PFHS /PFOS BACK TO MAIN HEM-SUM - 6 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT HAEMATOLOGY PARAMETERS ...................... PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 14.6 0.4 3 16.2 1.1 3 15.2 0.5 3 . 15.3 0.7 3 GROUP 3 TELOMER ACRYLATE GROUP'4 GROUP 5 PFHS 14.9 0.7 3 16.0 1.0 3 14.6 0.6 3 15.0 1.3 3 15.2 , 0.4 2 15.2 . 0.2 2 GROUP 6 PFOS 15.5 0.5 3 14.7 1.6 3 * I * * : Dunnett-test based on pooled variance sig. at 5% or 1%level. +: Steel-test sig. at 5% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS BACK TO MAIN HEM-SUM - 7 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS H..A.E.M..A..T.O..L.O.G..Y...P.A..R.AMETERS RBC MEAN 7.02 6.47 6.62 6.71 6.61 ' 6.53 T I1 ST.DEV. 0.09 0.19 0.23 0.51 0.35 0.33 N 3 3 3. 3 3 3 . HB MEAN 9.0 8.4 8.7 9.0 8.6 8.5 moll1 ST. DEV 0.2 0.2 0.3 0.4 0.5 0.4 N. 3 3 3 3 3 3 HCT 111 Mcv fl MCH fmol MCHC moll1 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N 0.404 0.009 3 57.5 0.7 3 1.277 0.021 3 22.2 0.2 3 0.378 0.005 3 58.4 1.7 3 1.305 0.060 3 22.4 0.4 3 0.389 0.012 3 58.9 2.8 3 1.310 0.055 3 22.3 0.2 3 0.396 0.012 3 59.2 2.7 3 1.339 0.048 3 22.6 0.4 3 0.383 0.030 3 57.9 2.1 3 1.301 0.041 3 22.5 0.5 3 0.387 0.016 3 59.3 0.6 3 1.307 0.012 3 22.1 0.2 3 ROW MEAN 14.5 15.2 15.4 15.3 14.6 15.7 x ST.DEV. 1.5 1.1 1.3 1.7 1.1 0.1 N 3 3 3 3 3 3 PLATELETS GI1 MEAN ST.DEV. N 1232 165 3 1117 144 3 1066 133 3 1111 854 56 145 3 3 1034 233 3 WBC MEAN 7.3 8.7 7.3 6.7 6.6 6.8 GI 1 ST.DEV. 1.8 2.8 3.3 1.3 1.0 2.5 N 3 3 3 3 3 3 SEG. MEAN 0.075 0.077 0.065 0.080 0.050 0.100 1 ST. DEV. 0.035 a. 020 0.018 0.033 0.030 0.015 N 3 3 3 3 3 3 EO. 1 . BAS0 1 'MEAN ST.DEV. N MEAN ST.DEV. N 0.005 0.005 3 0.000 0.000 3 0.007 0.008 3 0.000 0 9 000 3 0.003 0.003 3 0.000 0.000 3 0.010 0.005 3 0.000 0.000. 3 0.008 0.010 3 0.000 0.000 3 0.002 0.003 3 0.000 0.000 3 MONO. 1' MEAN ST.DEV. N 0.017 0.008 3 0.025 0.010 3 0.017 0.013 3 0.033 0.013 3 0.010 0.009 3 0.027 0.008 3 LYMPH. .MEAN 0.903 0.892 0.915 0.877 0.932 0.872 1 ST.DEV. 0.038 0.023 0.022 0.023 0.032 0.010 N. 3 3 3 3 3 3 * I * * :Ounnett-test based on pooled variance sig. at 5% or 1% level. +: Steel-test sig: at 5% level. 83 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - .. 'PFHS/PFOS HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 . BACK TO MAIN HEM-SUM - 8 09- DEC - 98 GROUP 5 PFHS GROUP 6 PFOS PT MEAN 16.0 14.9 15.6 14.8 * 14.8 15.5 SEC ST.DEV. 0.4 0.4 0.6 0.3 0.1 0.1 N 3 3 3 3 2 2 PTT MEAN 15.5 14.4 15.1 14.8 15.7 13.7 SEC ST.DEV. ~ 2.0 0.3 1..1 0.9 1.3 0.0 N 3 3 3 3 2 2 */": Dunnett-test based on pooled variance sig. at 5% or 1%level. t: Steel-test sig. at 5% level. 84 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE! -.- PFHSIPFOS BACK TO MAIN HEM-SUM - 9 0 -9DEC9- 8 HAEMATOLOGY SUMMARY ARER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS HAEMATOLOGY PARAMETERS ...-..._..-I__._..-_.- RBC MEAN 7.35 7.01 6.85 7.01 6.89 6.99 T I1 ST.DEV. 0.50 0.14 0.16 0.17 0.08 0.31 N 3 3 3 3 3 3 HB MEAN 9.1 9.1 9.1 9.2 9.0 9 .o mmolll ST.DEV. 0.6 0.2 0.3 0.3 0.4 0.2 N 3 3 3 3 3 3 MEAN ST.DEV. N 0.396 0.026 3 0.391 0.005. 3 0.384 0.012 3 0.392 0.015 3 0.390 0.014 3 0.389 0.015 3 MCV MEAN 53.9 55.8 56.1 55.8 56.7 55.7 1 fl ST.DEV. 1.6 0.8 1.6 0.8 1.4 1.8 N 3 3 3 3 3 3 MCH fho1 MEAN ST.DEV. N , 1.243 0.052 3 1.294 0.037 3 1.323 0.020 3 1.317 0.031 3 1.307 0.042 3 1.284 0.041 3 MCHC moll1 RDW % PLATELETS GI 1 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 23.1 0.3 3 14.5 0.4 3 1082 113 3. 23.2 0.3 3 12.9 ' 0.5 3 1025 95 3 23.6 0.4 3 13.7 0.3 3. 1076 149 3 23.6 0.6 3 12.9 ' 0.4 3 1179 70 3 23.1 0.2 3 13.6 0.3 3 966 71 3 23.1 0.3 3 13.7 0.9 3 1077 27 3 WBC G I1 SEG . 1 MEAN ST.DEV. N MEAN ST.DEV. N 32.2 0.9 3 0.103 0.053 3 12.3 3.0 3 0.095 0.028 3. 10.8 4.2 3 0.090 0.033 3 9.4 1.3 3 0.060 0.009 3 7.7 1.6 3 0.063 0.003 3 11.2 3.3 3 0.078 0.046 3 EO. 1 BAS0 . 1 MEAN ST.DEV. N MEAN ST.DEV. N 0.007 0.006 3 0.000 0 * 000 3 0.003 0.006 3 0.000 0.000 3 0.010 0.005 3 0.000 0.000 3 0.005 0.005 3 0.000 0.000 3 0.007 0.003 3 0.000 0.000 3 0.007 0.003 3 0.000 0.000 3' MONO. 1 MEAN ST.DEV. N 0.052 0.025 3 0.055 0.018 3 0.047 0.033 3 0.020 0.009 3 0.025 0.009 3 0.027 0.020 3 . LYMPH. MEAN 0.838 0.847 0.853 0.915 0.905 0.888 1 ST. DEV 0.056 0.050 0.068 0.005 0.013 0.059 N 3. 3 3 3 3 3 ' I " : Dunnett-test based on pooled variance s i g . at 5% or 1%level. +: Steel-test s i g . at 5% level. 85 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ :PFHS/PFOS HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 BACK TO MAIN HEM-SUM -io - 09-DEC 98 GROUP 5 PFHS GROUP 6 PFOS PT MEAN 13.1 13.0 13.4 13.8 14.2 14.0 SEC ST.DEV. 0.6 0.4 0.2 ~ 1.1 0.5 0.2 N 2 2 3 2 2 3 PTT MEAN 16 .O 15.2 16.8 16.5 15.2 14.6 SEC ST. DN. 0.1 0.8 0.2 0.8 0.4 2.0 N 2 2 3 2 2 3 * I * . : Dunnett-test based on pooled variance rig. at 5% or 1% level. +: Steel-test s i g . at 5% level. , 86 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - _* /PFHS/PFOS BACK TO MAIN HEM-SUM -11 ' 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER . ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS H..A.E.M..A.T..O.L.O..G.Y...P.A..R.A.METERS RBC MEAN 6.87 6.86 6.79 6.99 6.80 6.56 TI 1 ST.DEV. 0.19 0.09 0.27 0.22 0.25 0.15 N 3 3 3 3 3 3 HB moll1 HCT 111 MCV fl MCH fmol MCHC moll1 MEAN ST.DEV. N MEAN ST.DEV. N mm ST.DEV. N mm ST.DEV. N MEAN ST.DEV. N 9.3 0.1 3 0.397 0.023 3 57.8 1.8 3 1.349 0.020 3 23.4 1.0 3 9.4 0.2 3 0.402 0.002 3 58.7 1.1 3 1.366 0.018 3 23.3 0.4 3 9.0 . 0.2 3 0.389 0 .a09 3 57.4 2.3 3 1.322 0.033 3 23.1 0.4 3 9.1 0.2 3 0.393 0.008 3 56.2 0.9 3 1.297 0.030 3 23.1 0.2 3 8.9 0.3 3 0.389 0.022 3 57.2 3.4 3 1.314 0.049 3 23.0 0.5 3 8.7 0.6 3 0.379 0.030 3 57.8 3.3 3 1.321 0.058 3 22.9 0.6 3 RDW MEAN 12.8 13.5. 13.3 12.6 13.3 12.9 % ST.DEV. 0.8 0.5 0.2 0.3 1.1 0.9 N 3 3 3 3 3 3 . PLATELETS MEAN 972 980 965 1014 978 994 GI 1 ST. DEV 54 103 126 40 127 80 N 3 3 3 3 3 3. WBC G I1 SEG . 1 MEAN ST.DEV. N MEAN ST.DEV. N 6.0 0.6 3 0.053 0.031 3 8.8 1.1 3 0.070 0.013 3 7.7 3.7 3 0.038 0.015 3 6.2 0.4 3 0.077 0.045 3 6.8 2.3 3 0.075 0.017 3 8.7 2.3 3 0.062 0.025 3 EO. MEAN 0.008 0.003 0.010 0.020 0.007 0.005 1 ST.DEV. 0.010 0.003 0.000 0.000 0.003 0.009 N 3 3 3 3 3 3 BASO. 1 . MEAN ST. DEV N 0.000 0.000 3 0.000 0.000 3 0 IO00 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 MONO. 1 . MEAN ST. DEV N 0.015 0.005 3 0.027 0.008 3 0.018 0.014 3 0.030 0.000 3 0.035 0.018 3 0.017 0.008 3 LYMPH. 1 . MEAN ST.DEV. N 0.923 0.032 3 0.900 0.005 3 0.933 0.010 3 0.873 0.045 3 0.883 0.030 3 0.917 0.033 3 'I**: Dunnett-test based on pooled varfance s i g . at 5% or 1%level. t: Steel-test s i g . at 5% level. 87 NOTOX Project 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ /PFHSlPFOS BACK TO MAIN HEM-SUM -12 09 - D E C -98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL HAEMATOLOGY PARAMETERS .-......._._..._.._._I PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N 12.3 0.6 3 15.7' 1.2 3 GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 12.2 0.3 3 14.6 0.5 3 11.9 0.4 .3 15.7 1.1 3 12.2 0.6 3 15.0 0.6 3 GROUP5 PFHS 11.7 0.3 3 15.3 0.2 3 GROUP 6 PFOS 12.2 0.4 3 14.9 1.1 3 * l o * : Dunnett-test based on pooled variance sig. at 5% or 1% level. +: Steel-test s i g . at 5% level 88 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. c PFHS/ PFOS BACK TO MAIN CHE-SUM - 1 09-NOV-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS ~~ - ALAT( GPT) MEAN 0.64 0.61 0.61 0.57 0.67 0.59 ukatll ST.DEV. 0.12 0.10 0.14 0.11 0.25 0.06 N 14 14 13 13 14 14 ASAT( GOT) MEAN 2.80 2.62 2.58 2.31 *' 2.39 * 2.37 ukatll ST.DEV. 0.38 0.41 0.46 0.30 0.26 0.45 N 14 14 14 14 14 14 B I L I T. MEAN 2 2 2 2 2 2 umol I 1 ST.DEV. 1 0 1 0 0 1 N 14 14 14 14 14 14 CH0LEST.T. MEAN 2.00 1.28 * * 1.43 * * 1.76 0.97 " 1.13 ' molt1 ST.DEV. 0.39 0.44 0.47 0.40 0.20 0.40 I N 14 14 14 14 14 14 TRIGL. MEAN 0.80 0.63 0.73 0.65 0.43 *' 0.48 * * moll1 ST.DEV. 0.25 0.29 0.39 0.21 0.15 0.17 N 14 14 14 14 14 14 CREATININE M W 33. 30 31 34 35 32 umolll ST.DEV. 4 3 2 4 5 3 N 14 14 14 14 14 14 GLUCOSE MEAN 6.32 6.48 6.24 6.59 6.72 6.38 moll1 ST.DEV. 0.75 0.95 0.73 1.07 1.47 1.24 N 14 14 14 14 14 14 UREA MEAN 6.9 7.0 6.7 6.9 7.7 7.6 moll1 ST.DEV. 1.1 1.0 1.1 1.0 1.5 1.2 N 14 14 14 14 14 14 PROTEIN T. MEAN 56.8 53.2 * * 53.9 53.8 ' 53.7 * * 54.1 gll ST.DEV. 2.9 2.1 2.9 2.2 2.8 2.0 N 14 14 14 14 14 14 ALBUMIN MEAN 29.3 gll ST.DEV. 0.9 N 14 GLOBULIN MEAN 27.5 gll ST.DEV. 2.5 N 14 28.7 1.3 14 24.4 t 1.8 14 29.3 1.7 14 24.6 t 2.0 14 29.8 1.3 14 24.0 t -1.5 14 30.4 1.4 14 \ 23.3 t 2.4 14 29.3 1.2 14 24.8 + 2.1 14 AIG RATIO MEAN 1.1 1.2 1.2 t 1.2 t 1.3 t 1.2 t ST.DEV. 0.1 0.1 0.1 0.1 0.1 0.1 N 14 14 14 14 14 14 ALP MEAN 7.76 8.08 7.70 8.00 9.33 ' 7.26 ukatll ST.DEV. 1.75 1.94 1.14 1.16 1.50 1.48 N 14 14 14 14 14 14 * I t * : Dunnett-test based on pooled variance sig. at 5% or 1%l e v e l . t: Steel-test s i g . at 5% l e v e l . 89 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE] - - PFHS/PFOS BACK TO MAIN CHE-SUM - 2 09-NOV-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL . GROUP 2 TELOMER ALCOHOL INORG PHOSPH MEAN molll ST.DEV. N SODIUM molll MEAN ST.DEV. N POTASSIUM moll1 MEAN ST.DEV. N CALCIUM moll1 MEAN ST.DEV. N CHLORIDE moll1 MEAN ST. DEV. N 2.83 0.16 14 142.6 1.3 14 4. a5 0.31 14 2.53 0.08 14 100 2 14 2.75 0.13 14 ' ' 142.9 0.9 14 4.60 0.27 14 2.47 0.06 14 . 100 2 14 GROUP 3 TELOMER ACRYLATE GROUP 4 2.88 0.19 14 143.2 0.7 14 4.72 0.39 14 2.50 0.06 14 102 1 14 2.71 0.23 14 143.0 0.7 14 4.50 * 0.22 14 2.47 0.07 14 101 2 14 GROUP 5 PFHS 3.02, 0.30 14 143.3 0.8 14 4.55 0.46 14 2.51 0.08 14 103 * * 2 14 GROUP 6 PFOS 2.96 0.20 14 142.9 0.9 14 4.66 0.35 14 2.52 0.08 14 103 '* 3 14 * I r g : Dunnett-test based on pooled variance s i g . at 5% or 1% level. +: Steel-test s l g . at 5% level. 90 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ * PFHS/ PFOS BACK TO MAIN - CHE-SUM 3 - 09-NOV 98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TE.LOMER ACRYLATE GROUP 4 Gf?ouP 5 PFHS GROUP 6 PFOS ALAT(GPT) MEAN 0.52 ukatll ST.DN. 0.12 N 14 ASAT( GOT) MEAN 2.62 ukatll ST.DEV. 0.34 N 14 B I L I T. MEAN 3 umolll ST. DEV. 1 N 14 . CHOLEST T MEAN 2.21 moll1 ST.DEV. 0.44 N 14 TRIGL. MEAN 0.60 moll1 ST.DEV. 0.18 N 14 CREATININE MEAN 40 umolll ST.DN. 3 N 14 GLUCOSE mmolll UREA moll1 MEAN ST.DEV. N. MEM ST.DEV. N 6.05 0.52 14 8.3 1.8 . 14 I PROTEIN T. MEAN 65.9 g l1 ST.DN. 3.3 N 14 ALBUMIN ,. g l l MEAN ST.DEV. N 34.0 1.4 14 GLOBULIN MEAN 31.9 gl1 ST.DEV. 2.2 N 14 AIG RATXQ MEAN 1.1 ST.DEV. 0.1 N 14 . ALP MEAN 4.46 ukatll ST. DEV 0.62 N 14 0.47 0.09 , 14 2.37 0.53 14 2 1 14 2.26 0.40 14 0.57 0.18 14 38 4 14 5.90 0.58 14 8.4 1.5 14 64.8 2.8 14 33.8 1.4 14 31.0 ' 2.4 14 1.1 0.1 14 4.04 0.95 14 0.55 0.14 14 2.24 0.36 14 3 0 14 2.09 0.40 14 0.58 0.14 14 39 4 14 6.14 0.70 14 8.8 1.7 14 65.4 2.5 14 34.1 1.6 14 31.3 1.9 14 1.1 0.1 14 4.52 1.18 14 0.52 0.12 14 2.30 0.35 14 3 1 14 2.32 0.33 14 0.56 0.13 14 41 4 14 6.00 0.50 14 9.0 1.7 14 66.2 2.9 14 93.8 2.2 14 32.4 2.0 14 1.0 0.1 14 4.30 0.87 14 0.57 0.09 14 2.45 0.69 14 2 1 14 1.81 * 0.28 14 0.51 0.16 14 40 3 14 6.02 0.72 14 8.5 0.9 14 65.4 4.1 14 33.7 1.5 14 31.7 3.1 14 1.1 0.1 14 4.06 1.10 14 0.63 0.32 14 2.32 0.38 14 3 1 14 2.04 0.35 14 0.54 0.16 14 41 4 14 5.98 1.02 14 10.2 1.9 14 66.8 3.1 14 34.9 1.7 14 31.8 2.4 14 1.1 0.1 14 4.30 1.14 14 * I * * : Dunnett-test based on pooled variance s i g . at 5% or 1%level. +: Steel-test s i g . at 5% level. 91 NOTOX P r o j e c t 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ - a PFHSIPFOS BACK TO MAIN CHE-SUM - 4 0 9 - N O V -9 8 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL INORG PHOSPH MEAN moll1 ST.DEV. N SODIUM moll1 MAN ST.DEV. N POTASSIUM moll1 MEAN ST.DEV. N CALCIUM moll1 MEAN ST.DEV. N CHLORIDE moll 1 MEAN ST.DEV. N 2.58 0.23 14 142.2 1.6. 14 4.13 0.39 14 2.56 0.09 14 97 3 14 2.53 0.16 14 142.6 0.8 14 4.14 0.34 14 2.60 0.06 14 99 2 14 GROUP 3 TELOMER ACRYLATE 2.47 0.20 14 142.6 1.1 14 4.11 0.32 14 2.60 0.05 14 98 2 14 GROUP 4 ~~~ 2.42 0.19 14 142.0 1.0 14 4.19 0.24 14 2.60 0.05 14 99 2 14 GROUP 5 PFHS - 2.43 0.21 14 141.8 0.7 14 4.08 0.28 14 2.56 0.06 14 102 * * 2 14 GROUP 6 PFOS 2-.5_ 9_ 0.23 14 142.5 1.1 14 4.06 0.30 14 2.60 0.07 14 101 * * 2 4 * I * * : Dunnett-test based on pooled variance s i g . at 5% or 1% level. +: Steel-test s i g . at 5% level. 92 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - - ._ PFHS/PFOS BACK TO MAIN ' CHE-SUM - 5 0 9 - D E C -9 8 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT ' GROUP 1 GROUP 2 GROUP 3 GROUP 4 VEHICLE CONTROL TELOMER . ALCOHOL TELOMER ACRYLATE ALAT(GPT) ukatll ASAT ( GOT) ukatll B I L I T. umolll CH0LEST.T. mol/l TRIGL. mmol/l CREATININE umolll GLUCOSE moll1 UREA moll1 PROTEIN T. gl1 ALBUMIN 81 1 GLOBULIN gll AIG RATIO ALP ukatll MEAN ST.DEV. N MEAN * ST.DEV. N MEAN ST.DEV. N . MEAN ST. DEV N MAN ST.DEV. N MEAN ST. DEV N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN S T . DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 0.86 0.19 3 2.80 0.55 3 3 0 3 1.95 0.50 3 0.53 0.09 3 35 8 3 5.65 0.39 3 6.8 1.3 3 55.9 1.5 3 29'. 0 1.0 3 26.9 2.1 3 1.1 0.1 3 6.59 2.07 3 0.62 0.08 . 3 2.70 0.40 3 3 1 3 1.67 0.69 3 0.61 0.20 3 34 2 3 6.23 1.66 3 7.3 0.5 3 51.5 ' 1.0 3 28.1 0.5 3 23.4 0.8 3 1.2 0.0 3 6.93 1.02 3 0.65 0.12 3 2.91 0.03 3 3 1 3 1.57 0.04 3 0.89 0.35 3 33 2 3 6.85 0.93 3 6.9 0.7 3 57.6 0.9 '3 29.5 0.3 3 28.1 0.8 3 1.0 0.0 3 5.82 1.22 3 0.85 0.18 3 2.86 0.59 3 3 1 3 1.93 0.43 ., 3 0.70 0.10 3 39 2 3 8.33 0.11 3 6.8 0.5 3 53.6 3.2 3 27.8 . 1.3 3 25.8 2.1 3 1.1 0.1 3 5.94 1.02 3 GROUP 5 PFHS 0.81 0.13 3 2.43 0.42 3 2 0 3 1.38 0.22 3 0.65 0.09 3 39 4 3 7.14 0.16 3 6.7 0.8 3 54.7 0.7 3 28.9 1.0 3 25.8 0.9 3 1.1 0.1 3 6.72 0.80 3 GROUP 6 PFOS 0.64 0.17 3 1.92 0.48 3 3 1 3 1.27 0.19 3 '0.50 0.10 3 39 9 3 6.22 1.02 3 7.4 0.7 3 56.5 0.7 3. 29 -7 0.6 3 26.8 0.5 3 1 ..1 0.0 3 5.61 1.21 3 *I*': Dunnett-test based on pooled variance sig. at 5% or 1% level. +: Steel-test sig. at 5% level. 93 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/' - /PFHS/PFOS BACK TO MAIN CHE-SUM - 6 09-DEC- 98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY MALES , TREATMENT INORG PHOSPH MEAN moll1 ST.DEV. N SODIUM molll MEAN ST.DEV. N POTASSIUM molll MEAN ST.DEV. N CALCIUM moll1 MUIN ST.DEV. N CHLORIDE moll1 MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 2.81 0.06 3 144.3 1.1 3 4.46 0.42 3 2.48 0.04 3 103 1 3 GROUP 2 TELOMER ALCOHOL 2.78 0.16 3. 143.4 2.1 3 4.51 0.23 3 2.44 0.06 3 102 3 3 GROUP 3 TELOMER ACRYLATE GROUP 4 2.74 0.23 3 142.0 0.8 3 4.95 0.13 3 2.48 0.03 3 100 2 3 2.64 0.11 3 141.1 ' 0.7 3 4.41 0.20 3 2.50 0.11 3 100 1 3 GROUP 5 PFHS 2.51 0.14 3 142.4 0.9 3 4.56 0.15 3 2.45 0.04 3 102 2 3 GROUP 6 PFOS 2.65 0.12 3 142.'a 0.1 3 4.56 0.24 3 2.57 0.07 3 103 2 3 * I * * : Dunnett-test based on pooled variance s i g . a t 5% or 1% level. +: Steelatest sig. at 5% l e v e l . 94 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - - PFHS/PFOS BACK TO MAIN CHE-SUM - 7 09 -0EC- 98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT GROUP i VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 ALAT(GPT) ukatll MEAN ST.DEV. N. ASAT(GOT) ukatll . MEAN ST. DEV N B I L I T. umol I 1 MEAN ST.DEV. N CH0LEST.T. moll1 MEAN , ST.DEV. N TRIGL. moll1 . MEAN ST. DEV N CREATININE urnolll MEAN ST.DEV. N GLUCOSE mmol/l MEAN ST.DEV. N UREA moll1 MEAN ST.DEV. N PROTEIN T. gl1 . MEAN ST.OEV. n ALBUMIN ' gll MEAN ST. DEV. N GLOBULIN gll MEAN ST.DN. N AIG RATIO MEAN ST.0EV. N ALP . ukatll MEAN ST.DEV. N 0.47 0.05 3 2.53 0.58 3 3 1 3 2.16 0.38 3 0.69 0.24 3 39. 2 3 7.34 1.68 3 6.8 0.7 3 63.6 2.8 3 32.2 2.7 3 31.5 1.2 3 1.0 0.1 3 3.30 0.12 3 0.63 0.34 3 2.61 0.69 3 2 1 3 1.92 0.43 3 0.43 0.03 3 40 2 3 6.52 0.66 3 7.3 1.4 3 61.7 3.8 3 32.7 2.8 3 29.0 2.8 3 1.1 0.2 3 3.60 1.10 3 0.49 0.11 3 2.10 0.36 3 2 0 3 1.74 0.40 3 0.61 0.24 3 41 5 3 , 7.31 0.54 3 8.2 1.1 3 61.1 1.6 3 31.6 1.9 3 29.5 2.3 3 1.1 0.1 3 4.36 1.08 3 0.57 0.08 3 2.53 0.34 3 3 1 3 2.41 0.28 3 0.73 0.26 3 45 7 3 7.41 1.04 3 9.8 1.1 3 65.2 3.1 3 33.8 1.7 3 31.4 1.8 3 1.1 0.0 3 3.85 0.88 3 GROUP 5 PFHS GROUP 6 PFOS 0.52 0.12 3 0.45 0.07 3 1.66 0.15 3 2.09 0.17 3 3 2 0 1 3 3 2.11 0.40 3 2.02 0.37 3 0.40 ' 0.31 0.09 0.08 3 3 44 42 4 1 3 3 7.55 1.70 3 7.69 ' 0.33 3 7.6 8.0 0.4 1.2 3 3 66.4 4.2 3 60.6 5.2 3 31.4 32.5 1.o 1.2 3 3 35.1 3.3 3 28.1 4.0 3 0.9 1.2 0.1 0.1 3 3 3.27 1.20 3 4.32 1.51 3 */*e: Dunnett-test based on pooled variance sig. at 5% or 1%level. +: Steel-test s i g . at 5% level. 95 NOTOX Project 242933 TELOMER ALCOHOLlTELOMER ACRYLATE/' -- lPFHSlPFOS CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL INORG PHOSPH MEAN molll ST.DEV. N SOD I UM molll MEAN ST.DEV. N POTASSIUM molll MEAN ST.DEV. N CALCIUM molll MEAN ST.DEV. N CHLORIDE moll1 - MEAN ST.DEV. . N 2.47 0.09 3 142.4 1.0 3 4.08 0.09 3 2.63 0.14 3 99 1 3 . 2.57 0.36 3 142.8 1.3 3 3.96 0.20 3 2.59 0.06 3 99 1 3 GROUP 3 TELOMER ACRYLATE GROUP 4 2.32 0.06 3 142.0 1.0 3 4.05 0.42 3 2.57 0.09 3 99 4 3 2.55 0.27 3 141.3 1.5 3 4.34 0.38 3 2.68 0.03 3 98 2 3 BACK TO MAIN - CHE-SUM - 8 09 DEC - 98 GROUP 5 PFHS GROUP 6 PFOS 2.42 0.26 3 141.6 0.8 3 3.90 0.23 3 2.68 0.14 3 98 3 3 2.29 0.10 3 142.5 0.8 3 4.11 0.15 3 2.63 0.07 3 100 2 3 * I * . : Dunnett-test based on pooled variance sig. at 5% or 1% level. +: Steel-test sig. at 5% level. NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. L 'PFHS/PFOS CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL ALA1 (GPT) ukatll ASAT (GOT ukatll BILI T. umolll . CHOLEST.T moll1 TRIGL. molll CREATININE umolll GLUCOSE molll UREA moll1 PROTEIN T. 1 ALBUMIN 1 GLOBULIN gl1 AIG RATIO ALP ukat/l MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MCPN ST.ON. N MEAN ST.DEV. " MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N 0.59 0.17 3 2.65 0.25 3 3 1 3 1.94 0.18 3 0.76 0.39 3 36 1 3 8.01 0.40 3 6.3 0.3 3 62.2 0.1 3 28.6 0.6 3 33.5 0.6 3 0.9 0.0 3 4.33 0.65 3 0.68 0.07 3 2.44 0.25 3 2 1 3 1.25 0.13 3. 0.58 0.03 3 33 4 3 7.45 1.37 3 6.3 0.2 3 55.9 1.8 3 27.3 0.5 ' 3 28.6 2.3 3 1.0 0.1 3 6.20 1.91 3 GROUP 3 TELOMER ACRYLATE GROUP 4 0.60 0.03 3 2.06 0.24 3 3 1. 3 1.53 0.39 3 0.53 0.11 3 34 4 3 7.06 1.05 3 6.6 0.9 3 55.4 3.5 3 27.0 1.4 3 20.4 2.3 3 1.0 0.0 3 5.28 1.46 3 0.58 0.17 3 2.39 0.50 3 3 1 3 1.66 0.35 3 0.56 0.02 3 38 1 3 6.12 0.55 3 7.5 0.7 3 57.0 2.3 3 27.7 1.3 3 29.3 1.8 3 0.9 0.1 3 5.43 1.31 3 BACK TO MAIN - CHE-SUM - 9 09- NOV 98 GROUP 5 PFHS GROUP 6 PFOS 0.75 0.17 3 2.40 0.20 3 2 1 3 1.24 0.09 3 0.36 0.19 3 43 6 3 5.57 0.70 3 8.3 2.0 3 57.3 1.2 3 27.3 0.6 3 30.0 1.0 3 0.9 0.0 3 6.00 1.94 3 0.64 0.12 3 1.87 ' 0.13 3 2' 0 3 1.34 0.37 3 0.45 0.12 3 32 4 3 6.70 0.62 3 6.0 0.5 3 54.5 * * 2.5 3 27.5 0.7 3 26.9 2.0 3 1.0 0.1 3 4.73 0.19 3 * I * ' : Dunnett-test based on pooled variance sig. at 5% or 1%level. +: Steel-test sig. at 5% level. 97 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/. - 'PFHSIPFOS BACK TO MAIN CHE-SUM -10 09-NOV- 9 8 CLINICAL BIOCHEMISTRY SUMMA-RY AFTER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS INORG PHOSPH MEAN 3.02 2.64 2.64 2.46 * 2.65 2.59 molll ST.DEV. 0.13 0.28 0.24 0.18 0.18 0.20 N 3 3 3 3 3 3. SODIUM molll MEAN ST.DEV. N 141.6 0.9 3 142.9 1.3 3 142.5 1.2 3 142.0 0.6 3 143.8 1.0 3 142.7 0.2 3 POTASSIUM MEAN 5.33 4.59 4.64 4.55 *. 4.57 4.65 ' mmol/l ST.DEV. 0.21 0.14 0.33 0.12 0.42 0.11 N 3 3 3 3 3 3 ! CALCIUM MEAN 2.58 2.48 ' 2.48 2.46 ' 2.49 2.48 moll1 ST.DEV. 0.02 0.06 0.05 0.03 0.02 0.05 N 3 3 3 3 3 3 CHLORIDE MEAN 102 103 104 104 103 107 mol/l ST.DEV. 2 3 2 1 1 2 N 3 3' 3 3 3 3 ' / * * : Dunnett-test based on pooled variance slg. at 5% or 1% level. +: Steel-test s l g . at 5% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - IPFHSIPFOS BACK TO MAIN CHE-SUM -11 09-NOV-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 ALAT(GPT) ukatll ASAT(GOT) ukatll B I L I T. umolll CHOLEST. T. molll . TRIGL molll CREATININE umolll GLUCOSE mol11 UREA moll1 PROTEIN T. gll ALBUMIN gll GLOBULIN gll AIG RATIO ALP ukatll MEAN ST.DEV. . N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DEV. N 0.38 0.09 3 2.25 0.31 3 2 1 3 2.50 0.38 3 0.63 0.13 3 41 3 3 7.16 1.26 3 7.5 1.6 3 64.9 4.1 3 31.3 1.7 3 33.6 2.5 3 0.9 0.0 3 2.95 0.46 3 0.37 0.04 2 ' 2.31 0.33 3 2 1 3 2.41 0.36 3 0.74 0.27 3 42 5 3 6.43 0.47 3 7.5 1.3 3 ' 64.3 2.5 3 31.6 1.2 3 32.6 1.9 3 1.0 0.1 3 3.03 1.24 3 0.46 , 0.12 3. 1.89 0.45 3 3 1 3 2.30 0.11 3 0.47 0.04 3 42 3 3 6.90 0.37 3 8.0 1.0 3 67.7 2.2 3 33.4 3.4 3 34.4 1.5 3 1.0 0.1 3 3.11 0.92 3 ' 0.63 0.31 3 2.45 0.26 3 2 0 3 2.51 0.49 3 0.51 0.18 ' 3 44 4 3 6.34 1.56 3 8.3 1.5 3 65.3 3.4 3 30.6 1.3 3 34.7 2.1 3 0.9 0.0 3 2.93 0.85 3 GROUP 5 PFHS GROUP 6 PFOS 0.47 .0.16 3 0.50 0.02 3 2.52 0.49 3 1.92 0.05 3 2 2 0 1 3 3 2.06 0.35 3 2.13 0.22 3 0.40 0.06 3 0.50 0.21 3 39 39 1 1 3 3 6.86 0.41 3 7.65 1.09 3 7.0 10.7 0.3 3.7 3 3 69.4 0.9 3 34.2 0.7 3 65.3 5.4 3 32.8 2.0 3 35.2 1.4 3 32.5 3.3 ' . 3 1.0 1.0 0.1 . 0.0 3 3 2.70 0.85 3 3.38 0.19 3 .I.*: Dunnett-test based on pooled variance sig. at 5% or 1%level. +: Steel-test s i g . at 5% level. 99 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - - .. PFHS/PFOS BACK TO MAIN CHE-SUM -12 -09 NOV- 98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS INORG PHOSPH MEAN 2.33 2;24 1.98 2.22 2.16 2.27 moll1 ST.DEV. 0.17 0.12 0.20 0.30 0.08 0.26 N 3 3 ' 3 3 3 3 SODIUM moll1 . MEAN ST. DEV N 142.3 1.8 '3 141.6 0.5 3 140.7 1.7 3 141.3 0.7 3 141.0 1.4 3 141.1 1.6 '3 POTASSIUM MEAN 4.12 4.14 4.48 4.26 4.02 4.16 moll1 ST.DEV. 0.34 0.29 0.89 0.51 0.33 0.18 > N 3 3 3 3 3 3 CALCIUM MEAN 2.52 2.53 2.55 2.57 2.60 2.64 moll1 ST.DEV. 0.05 0.10 0.07 0.04 0.01 0.03 N 3 3 3 3 3 3 CHLORIDE MEAN 100 101 100 99 moll1 ST.DEV. 4 3 4 3 N 3 3 3 3 102 103 3 5 3 3 * I * * :Dunnett-test based on pooled variance s i g . at 5% or 1%level. +: Steel-test s f g . at 5% level. 100 NOTOX Project 242933 TELOMER ALCOHOLITELOMER ACRYLATE; .. d PFHS/PFOS BACK TO MAIN OW-SUM - 1 08-DEC - 98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS ~ ~ BODY W. MEAN 315 315 305 306 308 318 ST.DEV. 30 16 13 27 28 25 N 8 8 , 8 8 8 8 BRAIN MEAN ST.OEV. N 2.01 1.96 1.95 2.00 1.96 0.07 0.Q7 0.06 0.06 0.09 8 8 8 8 8 - 2.00 0.05 8 HEART MEAN 1.133 1.134 1.097 1.154 1.107 1.131 ST.DEV. 0.108 0.144 0.115 0.106 0.088 0.103 : N 888888 LIVER MEAN 10.05 10.35 9.36 9.04 13.78 * * 11.79 ST.DEv. 1.24 0.68 0.60 1.08 1.83 1.00 N 8 8 8 8 8 8 KIDNEYS MEAN 2.30 2.34 2.20 2.16 2.34 2.19 ST.DEV. 0.36 0.18 0.15 0.25 0.22 0.18 'N 8 8 8 8 8 8 ADRENALS MEAN ST.DEY. N 0.045 0.006 8 0.053 0.008 8 0.052 0.007 8 0.046 0.008 8 0.050 0.009 8 0.046 0.013 8 SPLEEN MEAN ST.DEV. N 0.580 0.119 8 0.566 0.069 8 0.567 0.065 8 0.578 0,075 8 0.570 0.076 8 0.558 0.096 8 TESTES MEAN 2.87 3.05 2.78 2.86 2.80 2.85 S i . DEV. 0.23 0.22 0.15 0.19 0.80 0.18 N 8 8 8 8 8 8 * / * * : Dunnett-test basad on pooled variance slg. at 5% or 1% level. 101 NOTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE1 .. d PFHSlPFOS BACK TO MAIN OW-SUM - 2 08-DEC-98 ORGANBODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 BRAIN I%) HEART (90 LIVER (90 KIDNEYS (%I ADRENALS (%I SPLEEN (%I TESTES (%I MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 315 30 8 0.64 0.05 8 0.362 0.037 8 3.19 0.21 8 0.73 0.09 8 0.014 0.002 8 0.183 0.027 8 0.91 0.05 8 315 16 8 0.62 0.03 8 0.361 0.046 8 3.30 0.29 8 0.74 0.06 8 0.017 0.003 8 . 0.180 0.017 8 0.97 0.09 8 305 13 8 0.64 0.03 8 0.360 0.037 8 3.07 0.11 8 0.72 0.05 8 0.017 0.002 8 0.186 0.015 8 0.91 0.06 8 306 27 8. 0.66 0.05 8 0.378 0.026 8 2.95 0.14 8 0.70 0.03 8 0.015 0.003 8 0.189 0.018 8 0.94 0.12 8 GROUP 5 PFHS GROUP 6 PFOS 308 28 8 0.64 0.05 8 318 25 8 . 0.63 0.05 8 0.360. 0.023 8 0.357 0.041 8 4.47 * * 0.40 8 3.71 *. 0.30 8 0.76 0.69 0.05 0.07 8 8 0.016 0.003 8 0.014 0.003 8 0.185 0.016 8 0.175 0.026 8 0.90 0.24 8 0.90 0.05 8 e/*.: Dunnett-test based on pooled variance s i g . at 5% or 1%l e v e l , NOTOX Project 242933 TELOMER ALCOHOLlTELOMER ACRYLATE / -. PFHSIPFOS BACK TO MAIN - OW-SUM 3 08 -DEC- 98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS FEMALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES MEAN ST.DEV. N MEAN ST.0EV. N MEAN ST.DEV, N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 214 22 8 1.88 0.08 8 0.852 0.054 8 6.62 0.50 8 1.50 0.15 8 0.060 0.009 8 0.423 0.051 8 0.124 0.023 8 211 ,16 8 ' 1.93 0.08 8 0.813 0.054 8 7.18 0.55 8 1.63 0.15 8 0.064 0.009 8 0.450 0.037 8 0.133 0.020 8 GROUP 3 TELOMER ACRYLATE GROUP 4 200 16 8 1.85 0.05 7 0.790 0.071 7 6.37 0.47 7 1.51 0.11 7 0.060 0.009 7 0.417 0.086 7 0.135 0.015 7 208 15 8 1.90 0.08 8 0.805 0.073 8 6.53 0.72 , 8 1.53 0.17 8 0.070 0.037 8 0.608 0.373 8 0.124 0.030 8 GROUP 5 PFHS 20 1 13 8 1.88 0.09 a 0.806 0.083 8 6.71 0.70 8 1.51 0.12 8 0.058 0.009 8 0.407 0.033 8 0.132 0.018 8 GROUP 6 PFOS 187 16 8 1.88 0.05 8 0.748 0.073 a 7.80 * * 0.88 8 1.46 0.12 8 0.054 0.006 8 0.412 0.046 8 0.128 0.019 8 * I * ' : Dunnett-test based on pooled variance s l g . at 5% or 1% level. .. NOTOX P r o j e c t 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ . PFHS/PFOS BACK TO MAIN OW-SUM - 4 08-DEC-98 ORGANBODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS BODY W. (GRAM) BRAIN (%I HEART LIVER (a) KIDNEYS (%I ADRENALS (%I SPLEEN (%I OVARIES (90 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 214 22 8 0.89 0.08 8 0.401 0.035 a 3.11 0.19 8 0.70 0.03 8 0.028 0.006 8 0.198 0 9 024' 8 0.058 0.010 0 211 200 16 . 16 8 8 0.92 0.05 8 0.93 0.06 .7 0.385 0.027 8 0.395 0.023 7 3.40 0.13 8 3.19 0.13 7 0.77 0.07 8 0.76 0.04 7 ' 0.031 0.005 ' a 0.030 0.002 7 0.214 0.020 8 0.208 0.037 7 0.063 0.012 a 0.068 0.008 7 208 15 8 0.91 0.04 8 0.388 0.030 8 3.14 0.28 a 0.74 0.08 8 0.033 0.016 8 0.298 0.201 8 0.060 0.015 a 201 13 8 0.94 0.05 .a 0.402 0.034 8 3.35 0.35 8 0.75 0.06 8 0.029 0.005 8 0.204 0.016 8 0.066 0.008 8 GROUP 6 PFOS 187 16 8 1.01 0.07 8 0.399 0.028 a 4.15 ' 0.18 8 0.78 0.03 8 0.029 0.003 8 0.220 0.013 8 0.069 0.013 8 . .. * / * * : Dunnett-test based on pooled variance s i g . a t 5% or 1% level. 104 NOTOX P r o j e c t 242933 - .. TELOMER ALCOHOL/TELOMER ACRYLATE/ . /PFHS/PFOS BACK TO MAIN - OW-SUM 5 08 -0EC- 98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT BODY W. MEAN ST.DEV. N BRAIN MEAN ST.DEV. N ' HEART i ! LIVER MEAN ST.DEV. N MEAN ST.DEV. N KIDNEYS MEAN ST.DEV. N ADRENALS MEAN ST.DEV. N SPLEEN MEAN ST.OEV. N TESTES MEAN ST.OEV. N GROUP 1 VEHICLE CONTROL 320 37 3 1.95 0.12 3 1.091 0.117 3 8.24 0.78 3 2.04 0.31 3 0.042 0.007 3 0.441 0.084 3 2.81 0.24 3 GROUP 2 TELOMER ALCOHOL 314 17 3 1.98 0.07 3 1.062 0.053 3 8.27 0.63 3 2.08 0.03 3 0.039 0.008 3 0.568 0.012 3 2.13 0.89 3 GROUP 3 TELOMER ACRYLATE GROUP 4 344 31 3 1.95 0.13 3 1.172 0.097 3 9.89 1.91 3 2.41 0.31 3 0.043 0.006 3 0.543 0.037 3 3.09 0.16 3 366 44 3 2.02 0.11 3 1.153 0.027 3 10.30 1.19 3 2.39 0.25 3 0.055 0.012 3 0.623 0.102 3 3.35 0.17 3 GROUP 5 PFHS 324 69 3 1.93 0.18 3 1.149 0.145 3 9.55 2.63 3 2.18 0.51 3 0.041 0.005 3 0.541 0.080 3 2.61 1.09 3 GROUP 6 PFOS 380 12 3 2.03 0.03 3 1.299 0.054 3 14.07 *' 0.63 3 2.65 0.06 3 0.048 0.007 3 0.635 ' 0,121 3 3.03 0.26 3 * / * ' : Dunnett-test based on pooled variance s l g . at 5% or 1%level. 105 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - IPFHSIPFOS BACK TO MAIN - OW-SUM 6 0 -8DE9C- 8 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT BODY W. (MAMI BRAIN (%I HEART (%I LIVER (XI KIDNEYS (%I ADRENALS (%I SPLEEN (%I TESTES (%I . MEAN ST. DEV N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 320 37 3 0.61 0.04 3 0.342 0.007 3 2.58 0.11 3 0.64 0.06 3 0.013 0.002 3 0.138 0.016 3' 0.88 0.04 3 GROUP 2 TELOMER ALCOHOL 314 17 3 0.63 0.04 3 0.339 0.029 3 2.63 0.07 3 0.66 0.05 3 0.013 0.003 3 0.181 0,011 3 0.68 0.30 3 GROUP 3 TELOMER ACRYLATE GROUP A 344 31 3 0.57 0.06 3 0.344 0.055 3 2.86 0.30 3 0.70 0.03 3 0.013 0.001 3 0.159 0.024 3 0.90 0.09 3 366 44 3 0.55 0.04 3 0.318 0.042 3 2.82 0.12 3 0.65 0.05 3' 0.016 0.005 3 0.170 0.007 3 0.92 0.06 3 GROUP 5 PFHS 324 69 3 0.61 0.09 3 0.361 0.045 3 2.93 0.20 3 0.67 0.04 3 0.013 0.001 3 0.169 0.019 3 0.78 0.22 3 GROUP 6 PFOS 380 12 3 0.53 0.02 3 0.342 0.009 3 3.70 '* 0.22 3 0.70 0.02 3 0.013 0.002 3 0.167 0.028 3 0.80 0.04 3 * / * * : Dunnett-test based on pooled variance 519. at 5% or 1% level. 106 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ . PFHSIPFOS BACK TO MAIN OW-SUM - 7 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT' GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.0EV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 239 29 3 1.96 0.10 3 o.89a 0.053 3 7.69 1.33 3 1.54 0.16 3 0.055 0.003 3 0.420 0.042 3 0.285 0.250 3 211 17 3 1.89 0.07 3 0.858 0.052 3 6.82 0.86 3 1.51 0.17 3 0.063 0.010 3 0.424 0,097 3 0.130 0.005 3 GROUP 3 TELOMER ACRYLATE GROUP 4 . GROUP 5 PFHS 202 14 3 1.91 0.03 3 0. a31 0.056 3 6.38 0.91 3 1.52 0.16 3 0.065 0.017 3 0.416 0.094 3' 0. I26 0.010 3 217 2a 3 1.91 0.03 3 0.936 0.052 3 7.07 0.09 3 1.60 0.12 3 0.069 0.005 3 0.414 0.016 3 0.145 0.020 3 224 11 3 1.92 0.02 3 0. a40 0.054 3 7.19 0.67 3 1.51 0.06 3 0,058 0.003 3 0.449 0.077 3 0.133 0.015 3 GROUP 6 PFOS 200 3 3 1.99 0.06 3 0.723 * * 0.018 3 7.55 0.56 3 1.38 0.11 3. 0.056 0.001 3 0.392 0.025 3 0.132 0.006 3 * / * * : Dunnett-test based on pooled variance s i g . a t 5% or 1% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE; + PFHSIPFOS ORGANBODY WEIGHT RATIOS SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT BODY W. (GRAM) BRAIN (%I MEAN ST.DEV. N. . MEAN ST.DEV. N HEART (%I MEAN ST.DEV. N LIVER (%I MEAN ST.DEV. N KIDNEYS (%I MEAN ST.DEV. N ADRENALS (%I MEAN ST.DEV. N SPLEEN (%I MEAN ST.DEV. N OVARIES ('lb) MEAN ST.DN. N GROUP 1 VEHICLE CONTROL 239 29 3 0.83 0.08 3 0.378 0.024 3 3.21 0.18 3 0.65 0 .a4 3 0.023 0.002 3 0.177 0.026 3 0.113 0.085 3 GROUP 2 TELOMER ALCOHOL 211 17 3 . 0.90 0.04 3 0.408 0.042 3 3.22 0.23 3 0.72 0.08 3 0.030 0.006 3 0.199 0.028 3 0.062 0.005 3 GROUP 3 TELOMER ACRYLATE GROUP 4 202 14 3 0.95 0.07 3 0.412 0.013 3 3.15 0.23 3 0.75 0.03 3 0.032 0.006 3 0.205 0.035 3 0.063 0.007 3 217 28 3 0.89 0.10 3 0.435 0.042 3 3.30 0.45 3 0.74 0.04 3 0.032 0.005 3 0.192 0.018 3 0.067 0.003 3 BACK TO MAIN OW-SUM - a' 08-DEC- 98 GROUP 5 PFHS GROUP 6 PFOS 224 11 3 0.86 0.04 3 0.375 0.008 3 3.21 0.23 3 0.68 0.06 3 0.026 0.001 3 0.202 0.041 3 0.059 0.004 3 200 3 3 1.00 0.03 3 0.362 0.007 3 3.78 0.32 3 0.69 0.06 3 0.028 0.001 3 0.197 0.015 3 0.066 0.004 3 - ' / * * : Dunnett-test based on pooled variance s i g . at 5% or 1% level. '. . .. 9 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .. 'PFHSIPFOS BACK TO MAIN OW-SUM - 9 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS BODY W. MEAN 389 363 378 401 335 344 ST.DEV. 28 33 3 36 20 38 N 3 3 . 3 3 3 3 BRAIN MEAN 1.97 2.05 ST.DEV. 0.15 0.04 N 3 3 2.09 0.03 3 2.11 0.19 3 2.02 0.08 3 2.00 0.07 3 HEART MEAN ST. DEV. N 1.196 0.093 3 1.207 0.018 3 1.217 0.077 3 1.299 0.147 3 1.048 0.153 3 1.231 0.248 3 3 ' LIVER MEAN ST.DEV. N 10.77 1.37 3 9.78 0.44 3 10.30 0.69 3 10.06 0.35 3 8.84 0.63 3 11.17 1.94 3 KIDNEYS MEAN 2.44 2.42 2.51 2.59 2.24 2.48 ST.DEV. 0.23 0.06 0.04 0.24 0.41 0.21 N 3 3 3 3 3 3 ADRENALS MEAN ST.DEV. N 0.046 0.011 3 0.050 0.009 3 0.054 0.004 3 0.056 0.013 3 0.047 0.003 3 0.046 0.009 3 SPLEEN MEAN ST.DW. N 0.614 0.040 3 0.540 0.082 3 0.591 0.061 3 0.720 0.037 3 0.552 0.065 3 0.629 0,136 3 TESTES MEAN 2.86 3.19 3.11 3.10 3.09 3.27 ST.DEV. 0.14 0.43 0.06 0.22 0.11 0.30 N 3 3 3 3 3 3 * / * * : Dunnett-test based on pooled variance s i g . at 5% or 1% level. NOTOX Project 242933 TELOMER .ALCOHOL /TELOMER ACRYLATE/ IPFHSIPFOS ORGANBODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT BRAIN HEART (%I LIVER (%I KIDNEYS (%I ADRENALS SPLEEN (%I TESTES (%I MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. , N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST. OW. N MEAN ST.OEV. N GROUP 1 VEHICLE CONTROL 389 28 3 0.51 0.03 3 0.308 0.027 3 2.76 0.17 3 0.63 0.05 3 0.012 0.003 3 0.159 0.017 3 0.74 0.04 3 GROUP 2 TELOMER ALCOHOL 363 33 3 0.57 0.04 3 0.335 0.030 3 2.70 0.13 3 0.67 0.04 3 0.014 0.002 3 0.149 0.019 3 0.88 0.14 3 GROUP 3 TELOMER ACRYLATE GROUP 4 378 3 3 0.55 0.01 3 0.322 0.018 3 2.72 0.18 3 0.66 0.01 3 0.014 0.001 3 0.156 0.016 3 0.82 0.02 3 401 36 3 0.53 0.03 3 0.324 0.016 3 2.52 0.21 3 0.65 0.08 3 0.014 0.002 3 0.181 0.026 3 0.78 0.05 3 BACK TO MAIN - OW-SUM 10 08-DEC - 98 GROUP 5 PFHS GROUP 6 PFOS 335 20 3 0.60 0.01 3 0.313 0.042 3 2.64 0.03 3 0.67 0.14 3 0.014 0.000 3 0.164 0.010 3 0.92 0.02 3 344 38 3 0.59 0.05 3 0.356 0.035 3 3.23 0.22 3 0.72 0.02 3 0.014 0.004 3 0.182 0.022 3 0.95 ' 0.08 3 * I * * : Dunnett-test based on pooled varfance s l g . a t 5% or 1%level. 110 I NOTOX Project 242933 -- TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS BACK TO MAIN ow-SUM - 11 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT BODY w. BRAIN HEART LIVER KIDNEYS ADRWALS SPLEEN OVARIES MEAN ST.DEV. N MEAN ST.0EV. 'N MAN ST. DEV. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 243 27 3 1.91 0.09 3 0.916 0.118 3 7.01 1.14 3 1.60 0.25. 3 0.065 0.012 3 0.445 0.056 3 0.133 0.013 3 GROUP 2 TELOMER ALCOHOL 228 11 3 1.97 0.06 3 0.851 0.109 3 6.87 0.57 3 1.63 0.09 3 0.064 0.013 3 0.443 0.140 3 0.146 0.029 3 t GROUP 3 TELOMER ACRYLATE GROUP 4 226 25 3 1.88 0.06 3 0.870 0.046 3 6.85 0.86 3 1.54 0.14 3 0.062 0.008 3 0.426 0.065 3 0.145 0.013 3 235 35 3 1.96 0.05 3 0.918 0.092 3 7.30 1.56 3 1.68 0.25 3 0.061 0.001 3 0.504 0.104 3 0.165 0.040 3 GROUP 5 PFHS . 226 13 3 1.88 0.08 3 0.860 0.065 3 6.83 0.20 3 1.62 0.12 3 0.065 0.003 3 0.355 0.024 3 0.153 0.047 3 GROUP 6 PFOS 208 11 3 1.88 0.09 3 0.825 0.028 3 7.49 0.30 . 3 1.68 0.12 3 0.058 0.010 3 0.427 0.060 3 0.141 0.003 3 * / * * : Dunnett-test based on pooled variance sig. at 5% or 1% level. 111 NOTOX P r o j e c t 242933 TELOMER ALCOHOLlTELOMER ACRYLATE/ -- . PFHSIPFOS BACK TO MAIN OW-SUM - 12 0 -8DEC9- 8 ORGANBODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL BODY W. (GRAM) BRAIN (%I HEART LIVER (W KIDNEYS (%I ADRENALS (96) SPLEEN (%I OVARIES (%I MEAN ST.DN. N MEAN ST.DN. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DN. N 243 27 3 0.79 0.08 3 0.379 0.039 3 2.88 0.20 3 0.66 0.04 3 0.027 0.005 3 0.184 0.023 3 0.055 ' 0.009 3 228 11 3 0.86 0.03 3 0.372 0.029 3 3.02 0.21 3 0.72 0.07 3 0.028 0.006 3 0.193 0.052 3 0.064 0.010 3 GROUP 3 TELOMER ACRYLATE GROUP 4 226 25 3 0.84 0.06 3 0.387 0.021 3 3.03 0.14 3 0.69 0.07 3 0.028 0.004 3 0.192 0.046 3 0.065 0.011 3 235 35 3 0.85 0.12 3 0.392 0.021 3 3.09 0.29 3 0.71 0.05 3 0.026 0.003 3 0.216 0.048 3 0.070 0.012 3 GROUP 5 PFHS 226 13 3 0.83 0.01 3 0,380 0.017 3 3.02 0.17 3 0.72 0.08 3 0.029 0.001 3 0.157 0.019 3 0.068 0.023 3 GROUP 6 PFOS 208 11 3 0.90 0.04 3 0.398 0.032 3 3.61 *' 0.20 3 0.81 0.03 3 0.028 0.006 3 0.206 0.031 3 0.068 0.002 3 * / * * : Dunnett-test based on pooled variance s l g . at 5% or 1%level. 112 NOTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ ' HISTORICAL DATA HAEMATOLOGY ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES PARAMETER SEX N MEAN RBC M TI 1 F HB M mol I 1 F HCT M 111 F MCV M fl F MCH M fmol F MCHC M moll1 F PLATELETS M G/ 1 F RDW M x F WBC M 61 1 F SEG M l(re1) F EO l(re1) BAS0 l(re1) LYMPH l(re1) MONO m l(re1) F PT M sec F PTT M sec F 50 44 50 44 ' 50 44 50 . 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 44 37 44 36 7.08 6.63 9.8 9.5 0.415 0.386 58.6 58.3 1.387 1.441 23.7 24.7 1137 1114 12.2 11.7 11.8 9.2 0.11 0.097 0.006 0.008 0 0 0.855 0.87 0.028 0.025 13.2 13.9 16.5 15.5 * .. PFHS/PFOS ST. DEV. MIN. 0.36 0.32 0.5 0.3 0.019 0.016 1.8 1.4 0.073 0.055 1.1 0.6 148 139 0.7 0.6 2.7 2.6 0.049 0.065 0.007 0.008 0 0 0.061 0.07 0.021 0.020 1 0.9 1.8 1.9 6.42 6 8.8 8.9 0.384 0.353 55 55.4 1.245 1.345 21.1 23.5 760 753 11.1 10.6 4.7 5.3 0.015 0.02 0 0 0 0 0,705 0.705 0 0 11.2 12 11.9 10.3 MAX. 7.95 7.5 10.8 10.4 0.463 0.437 63.6 60.7 1.52 1.584 25.5 26.3 1486 15 13 14.4 13.1 17.5 18.7 0.235 0.27 0.02 0.03 0 0 0.985 0.965 -0.085 0.075 15.5 15.7 19.7 19.4 BACK TO MAIN HIS-SUM - 1 22-APR-99 Data are from Sprague Dawley control rats whlch received the vehicle (in general 0.9% NaC1) Via continuous intravenous infusion,.Due to the limited availability of data no confidence intervals were calculated. 113 NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ ' HISTORICAL DATA HAEMATOLOGY ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES - .. PFHSl P F O S PARAMETER SEX N MEAN ST. DEV. MIN. MAX. RBC 42 TI1 30 HB 42 moll1 30 HCT M 42 111 F 30 MCV M 42 P1 F 30 ! MCH M 42 fmol F 30 MCHC M 42 moll1 F 30 PLAELETS M 42 011 . F 30 RDW M 42 % F 30 WBC M 42 Gl 1 F 30 SEG M 42 l(re1) F. 30 EO M 42 I l(re1) F 30 8 i BAS0 H 42 l(re1) . f . 30 , LYMPH M 42 l(re1) F 30 MONO M 42 l(re1) F 30 PT M 38 ret F 29 PTT M 38 sec F 29 7.34 6.81 9.3 8.8 0.399 0.376 54 55.2 1.251 1.3 23.1 23.6 1096 1130 14.6 12.9 14.2 8.4 0.157 0.148 0.009 0.008 0 0 0.803 0.823 0.031 0.020 14.2 13.8 16.4 16.3 0.73 0.47 I.3 0.6 0.047 0.027 2.9 2.1 0.087 0.054 0.8 0.5 170 145 1 .a 0.8 5.6 2 0.093 0.102 0.009 0.008 0 0 0.108 0.105 0.025 0.016 1.3 1 2.1 1.9 5.59 5.59 5.6 7 0.264 0.313 45.3 51.5 0.961 1.19 21.2 22.4 669 855 12.7 11.6 6.1 4.7 0.04 0.04 0 0 0 0 0.45 0.54 0 0 11.9 11.9 11.6 12.3 8.7 7.65 11 9.9 0.472 0.423 59.6 59.8 1.391 1.42 24.4 24.6 1455 1515 20.5 15.2 33.9 12 0.505 0.42 0.035 0.03 0 0 0.935 0.945 0.105 0.065 17.3 15.6 21.2 19.6 BACK TO MAIN HIS-SUM - 2 22-APR-99 ' Data are from Sprague Dawley control rats which received the vehicle (in general 0.9% NaC1) via continuous intravenous infusion. Due to the limited availability of data no confidence intervah were calculated. 114 .: NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE PFHS/PFOS ' HISTORICALDATA CLINICALBIOCHEMISTRY ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES BACK TO MAIN HIS-SUM - 3 22-APR-99 PARAMETER SEX N MEAN ST. O N . MIN. MAX. ALATIGPT M 51 Mat1 1 F 45 0.66 0.11 0.45 0.98 0.54 0.15 0.24 1.06 ASATI G O T M 51 Mat1 1 F 45 2.51 0.6 1.54 3.8 2.46 0.43 1.69 3.48 BILI T. M 51 2 poll1 F 45 3 1 1 4 1 '2 5 CHOLEST. T. M 51 moll1 F 45 . TRIGL moll1 M 51 F 45 1.95 2.05 0.69 0.42 0.34 0.4 0.37 0.09 1.25 1.08 0.34 0.26 2.98 3.31 2.11 0.68 CREATININE M 51 33 6 poll1 F 45 40 6 25 54 31 56 GLUCOSE moll1 M 51 F 45 6.03 1.07 3.86 8.45 5.32 0.68 9.87 7.4 UREA moll1 M 51 F 45 6.1 1.6 3 8.9 8.1 2.1 4.5 12.8 PROTEIN T. M 51 gll F 45 64.1 2.9 67.4 4.2 57.7 70.9 59.5 75.5 ALBUMIN . gll M 51 F 45 29.5 1.8 32.6 2 24.5 32.8 28.4 36.6 GLOBULIN M 51 911 F 45 34.6 2.5 34.8 4.5 29.1 40.9 2s.a 43.7 AIG RATIO M 51 0.9 0.1 0.6 1 i 1 F 45 1 0.1 0.7 1.3 ALP pkatll M 51 F 45 7.38 1.87 2.94 12.3 4.3 1.19 2.63 7.86 SODIUM mol I1 M 51 F 45 143.3 1.6 141.2 1.6 138.5 137.5 146.3 144.8 POTASSIUM M 51 4.83 0.43 3.35 5.97 mm0lIl F 45 I 4.59 0.3 3.97 5.32 CHLORIDE M 51 moll1 F 45 100 3 101 3 95 106 94 ll0 Data are from Sprague Dawley control rats which received the vehicle (in general 0.9% NaC1) Via , continuous intravenous infusion. Due to the limited availability of data no confidence intervals were calculated, NOTOX P r o j e c t 242933 TELOMER ALCOHOL /TELOMER ACRYLATE - PFHS1PFOS ' HISTORICALDATA CLINICAL BIOCHEMISTRY ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES PARAMETER SEX N MEAN ST. OW. MIN. MAX. CALCIUM M 51 moll1 F 45 2.6 0.09 2.41 2.8 2.67 0.09 2.46 2.86 INORG. PHOSPH. M 51 ' 2.78 0.21 2.29 3.27 m o lI1 F 45 2.55 0.25 2.09 3.38 BACK TO MAIN HIS-SUM - 4 2 2 - A P R -99 Data are from Sprague Dawley control fats whlch received the vehicle ( i n general 0 . B NaC1) via contlnuous intravenous infusion. Due to the llmlted availability of data no confidence intervals were calculated, 116 BACK TO MAIN NOTOX P r o j e c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS ' HISTORICALDATA CLINICAL BIOCHEMISTRY ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES - HIS-SUM 5 -22 APR- 99 PARAMETER SEX N MEAN ST. DEV. MIN. MAX. I I I , ./ . ALATIGPT M 42 pkatl 1 F 28 ASATIGOT M 42 pkatll F 28 BILI T. plolll M 42 F 28 CHOLEST. T. M 42 mmolll F 28 . TRIGL moll1 M 42 F 28 CREATININE M 41 plolll F 28 GLUCOSE molll M 42 F 28 UREA mmolll M 46 F 32 PROTEIN T. M 42 gl1 F 28 ALBUMIN gll M 42 F 28 GLOBULIN M 42 gll F 28 A/G RATIO 'M 42 1 F 28 ALP pkatll M 42 F 28 SODIUM molll M 42 F 28 POTASSIUM M 42 . molll F 28 CHLORIDE M 42 moll1 F 28 o.n 0.64 - 2.64 2.31 2 3 1.93 2.49 0.58 0.47 37 42 . 6.38 5.93 5.5 5.7 66.4 72.5 28.1 32 38.2 40.5 0.7 0.8 4.21 3.32 142 140 5 4.43 101 103 0.38 0.19 0.55 0.42 1 1 0.43 0.45 0.25 0.19 4 5 1.22 0.71 1.4 1.5 3.8 3.5 3 3.8 4.2 4.4 0.1 0.2 1.04 2.51 2.4 2.7 0.36 0.29 3 3 0.47 0.33 1.65 1.45 1 2 0.98 1.69 0.23 0.27 27 35 4.21 5.04 3 3.7 56 66.8 21 23.9 31 34.4 0.4 0.5 2.45 1.15 137.1 135.8 4.38 3.87 96 98 2.98 1.15 4.05 3.09 4 5 2.8 3.27 1.13 1.24 46 52 10.65 7.43 8.5 9.6 74 81 32.8 38.2 49 50.8 1 1.1 , 7.55 14.54 148.3 147.1 5.81 5.19 107 110 ' Data are from Sprague Dawley control rats which received the vehicle (in general 0.9% NaC1) Via continuous Intravenous infusion. Due to the limited availability of data no confidence interVal. were calculated, 117 \. .. NQTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE PFHS/PFOS ' HISTORICALDATA CLINICALBIOCHEMISTRY ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES PARAMETER SEX N MEAN ST. ON. MIN. MAX. CALCIUM moll 1 M 42 F 28 INORG. PHOSPH. M 42 moll1 F 28 2.53 2.5 2.6 2.1 0.14 0.17 0.24 0.23 2.23 2.26 2.17 1.65 2.78 2.8 3.34 2.56 BACK TO MAIN HIS-SUM - 6 22-APR-99 I ' Data are from Sprague Dawley Control rats which received the vehicle ( I n general 0.9% NaCl) via contlnuous intravenous infusion. Due to the limlted avallablllty of data no confidence Intervals Were calculated. 118 .. TELOMER ALCOHOL/TELOMER ACRYLATE/ lPFHSlPFOS BACK TO MAIN NOTOX 242933 TABLES INDIVIDUAL DATA 119 NOTOX Project 242933 TELOMER ALCOHOLlTELOMER ACRYLATE - .. PFHSlPFOS MORTALITY DATA MALES GROUP 1 (VEHICLE CONTROL) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 1 03-NOV-98 2 03-NOV-98 3 03-NOV-98 4 03-NOV-98 5 04-NOV-98 6 04-NOV-98. 7 04-NOV-98 8 04-NOV-98 9 18-NOV-98 10 18-NOV-98 11 18-NOV-98 13 02-OEC-98 14 02-DEC-98 15 02-DEC-98 06-OCT.98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 '06-OCT-98 06-OCT.98 06 -0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 02-NOV-98 02-NOV-98 02-NOV- 98 02-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03 -NOV. 98 03-NOV-98 03-NOV-98 03-NOV-98 MALES GROUP 2 (TELOMER ALCOHOL) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 17 03-NOV-98 18 03-NOV-98 - 19 03-NOV-98 20 03-NOV 98 04-NOV- 98 04-NOV-98 23 04-NOV-98 24 04-NOV-98 - 25 18-NOV-98 26 18 NOV-98 27 18-NOV-98 29 02-DEC-98 30 02-DEC-98 3 1 02-DEC-98 , . 06-0CT-98 02-NOV-98 . 06-OCT-98 02-NOV-98 - - 06-OCT-98 02-NOV-98 06 OCT 98 02-NOV-98 06-OCT-98 03-NOV-98 - - 06-0CT-98 06 OCT 98 06-0CT- 98 06 -0CT-98 06-OCT-98 06-OCT-98 03-NOV- 98 03-NOV- 98 03-NOV-98 - 03-NOV 98 - 03-NOV 98 03-NOV-98 06-0CT-98 03-NOV- 98 06-OCT-98 03-NOV-98 06-OCT-98 03-NOV-98 BACK TO MAIN - MORT-IND 1 - 24-FEB 99 120 NOTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ PFHSIPFOS- .. MORTALITY DATA MGRAOLEUSP 3 (TELOMER' ACRYLATE) SCHEDULED ANIMAL NECROPSY OTHER TfEATMENT FROM TO 33 03-NOV-98 34 03-NOV-98 35 03-NOV-98 - - 36 03-NOV-98 37 04 NOV 98 38 04- NOV -98 39 04-NOV-98 - - 40 04 NOV 98 41 18-NOV-98 - - 42 18 NOV 98 43 18-NOV-98 45 02-DEC-98 46 02- DEC-98 47 02-DEC-98 06- OCT-98 06-OCT-98 - - 06 OCT 98 -06 OCT-98 -06 OCT-98 06-OCT-98 06-OCT- 98 06-OCT-98 06-OCT-98 - - 06 OCT 98 - - 06 OCT 98 06-OCT-98 -06-OCT-98 06 OCT-98 02-NOV-98 - - 02-NOV-98 02 NOV 9a 02-NOV- 98 03-NOV- 98 - 03-NOV 98 03-NOV-98 - 03-NOV 98 03-NOv- 98 - 03-NOV 98 03-NOV-98 - 03-NOV-98 03-NOV 98 03-NOV- 98 I MALES GROUP 4 SCHEDULED ANIMAL NECROPSY - - 49 03 NOV 98 50 03-NOV-98 51 03-NOV-98 52 03-NOV-98 53 04-NOV-98 - 54 04- NOV-98 55 04-NOV 98 56 04-NOV-98 57 18-NOV-98 58 18-NOV-98 59 18-NOV- 98 61 02-DEC-98 62 02- DEC-98 63 02-DEC-98 OTHER .. TREATMENT FROM TO 06- OCT-98 06 -0CT-98 - 06-0CT-98 06- OCT 98 06 -0CT-98 06 -0CT-98 06 -0CT -98 06 -0CT-98 06-OCT- 98 06-OCT-98 06 -0CT-98 06 -0CT- 98 06 *OCT-98 06-OCT-98 - 02-NOV-98 02-NOV 98 02-NOV- 98 02-NOV -98 03-NOV-98 03-NOV- 98 03-NOV -98 -03.NOV- 98 03 NOV- 98 03-NOV- 98 03-NOV-98 03-NOV- 98 03-NOV- 98 03-NOV- 98 BACK TO MAIN -- MORT-IND 2 24 * FEB 99 121 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE / .. PFHSIPFOS- MORTALITY DATA MALES GROUP 5 (PFHS) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 65 03-NOV-98 66 03-NOV-98 67 03-NOV-98 68 03-NOV-98 69 04-NOV-98 70 04-NOV-98 71 04-NOV-98 72 04-NOV-98 73 18-NOV-98 74 18-NOV-98 75 18-NOV-98 77 02-DEC-98 I 78 02-DEC-98 79 02-DEC-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 02-NOV-98 02-NOV-98 02-NOV-98 02-NOV-98 03-NOV-98 03-NOV-98 , 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 MALES I GROUP 6 (PFOS) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO - 81 03-NOV-98 82 03-NOV 98 83 03-NOV-96 - 84 03-NOV-98 85 04-NOV 98 ' 86 ,04-NOV-98 87 04-NOV-98 88. 04-NOY-98 89 18-NOV-98 90 18-NOV-98 91 18-NOV-98 93 02-DEC-98 94 . 02-DEC-98 95 02-MC-98 -06 OCT-98 02-NOV-98 06-OCT-98 02-NOV-98 - 06-0CT-98 02-NOV 98 -06 OCT-98 02-NOV-98 -06 OCT-98 03-NOV-98 06-0CT-98 03-NOV-98 06-OCT-98 03-NOV-98 06-OCT-98 03sNOV-98 06-0CT-98 03-NOV-98 06-OCT-98 03-NOV-98 06-OCT-98 03-NOV-98 06-OCT-98.03-NOV-98 06bOCT-98 03-NOV-98 06-0CT-98 03-NOV-98 BACK TO MAIN MORT-IND - 3 24-FEB-99 122 NOTOX P r o j e c t 242933 TELOMER ALCOHOLITELOMER ACRYLATE1 PFHS/PFOS- MORTALITY DATA FEMALES GROUP 1 (VEHICLE CONTROL) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 97 05-NOV-98 98 05-NOV.98 99 05-NOV-98 100 05-NOV-98 101 06-NOV-98 102 06-NOV-98 103 06-NOV-98 104 06-NOV-98 105 20-NOV-98 106 20-NOV-98 107 20-NOV-98 109 04-DEC-98 i 110 04-OEC-98 I 111 04-DEC-98 08-OCT-98 -08-OCT-98 08 OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08.OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 04-NOV-98 - - 04-NOV-98 04 NOV 98 04-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV- 98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 . FEMALES GROUP 2 (TELOMER ALCOHOL) SCHEDULED ANIMAL NECROPSY OMER TREATMENT FROM TO - 113 05-NOV 98 - 114 05-NOV 98 115 05-NOV-98 116 05- NOV- 98 117 06-NOV-98 118 06-NOV-98 - 119 06-NOV-98 120 06-NOV 98 121 20-NOV-98 122 20-NOV-98 123 20-NOV-98 125 04-DEC-98 126 04-DEC-98 127 04-DEC-98 08-OCT-98 08-OCT-98 08-OCT-98 08.OCT-98 08 -0CT- 98 08 -0CT-98 08.OCT- 98 08-OCT-98 08 OCT- 98 08-OCT-98 08 -0CT-98 08 -0CT-98 08-OCT-98 08 -0CT-98 04- NOV- 98 04-NOV- 98 04-NOV-98 04-NOV-98 05-NOV-98 05-NOV- 98 05-NOV-98 05-NOV-98 - - 05-NOV-98 05 NOV 98 05-NOV- 98 05-NOV-98 05-NOV- 98 05-NOV- 98 BACK TO MAIN MORT-IND - 4 24-FEB- 99 123 NOTOX Project 242933 .. TELOMER ALCOHOL ITELOMER ACRYLATE/ !PFHS / PFOS- MORTALITY DATA FEMALES . GROUP 3 (TELOMER ACRYLATE) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 129 130 05-NOV-98 - 05-NOV 98 - 08-OCT-98 04-NOV 98 08-OCT-98 04-NOV-98 131 05-NOV-98 08-OCT-98 04-NOV-98 132 05-NOV-98 08-OCT-98 04-NOV-98 133 06-NOV-98 08-OCT-98 05-NOV-98 134 06-NOV-98 08-OCT-98 05-NOV-98 135 06-NOV-98 08-OCT-98 05-NOV-98 136 06-NOV-98 08-OCT-98 05-NOV-98 137 20-NOV-98 138 20-NOV-98 . '- 08- OCT-98 05-NOV-98 08-0CT-98 05-NOV-98 139 20-NOV-98 08-OCT-98 05-NOV-98 141 04-DEC-98 08-OCT-98 05-NOV-98 142 04-DEC-98 08-OCT-98 05-NOV-98 143 04-DEC-98 08-OCT-98 05.NOV-98 FEMALES GROUP 4 SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 145 05-NOV-98 146 05-NOV-98 147 05-NOV-98 148 05-NOV-98 149 06-NOV-98 150 06-NOV-98 151 06-NOV-98 152 06-NOV-98 153 20-NOV-98 154 20-NOV-98 - 155 20-NOV-98 157 04-DEC 98 158 04-DEC-98 159 04-DEC-98 08-OCT-98 08- OCT- 98 08-OCT-98 08-OCT-98 08- OCT-98 -08-OCT-98 08 OCT-98 08-OCT-98 08- OCT-98 08 -0CT-98 08-OCT-98 08-OCT-98 08 * OCT-98 08- OCT- 98 04-NOV-98 04-NOV-98 04-NOV-98 04-NOV- 98 - 05-NOV-98 05-NOV 98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 - 05-NOV 98 05-NOV-98 - - 05 NOV 98 05-NOV-98 BACK TO MAIN - MORT-IND 5 24-FEB -99 124 NOTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE / - .. 'PFHS /PFOS MORTALITY DATA FEMALES GROUP 5 (PFHS) SCHEDULED ANIMAL NECROPSY OTHER 161 05-NOV-98 162 05-NOV-98 163 05-NOV-98 164 05-NOV-98 165 06-NOV-98 166 06-NOV-98 167 06-NOV-98 168 06-NOV-98 169 20-NOV-98 170 20-NOV-98 171 20-NOV-98 173 04-DEC-98 174 04-DEC-98 175 04-DEC-98 FEMALES GROUP 6 (PFOS) SCHEDULED ANIMAL NECROPSY - 177 05 NOV.98 178 05-NOV-98 179 05-NOV-98 180 05-NOV-98 181 06-NOV-98 182 06-NOV-98 183 06-NOV-98 184 06-NOV-98 185 20-NOV-98 186 20-NOV-98 187 20-NOV-98 - 189 04-DEC 98 190 04-DEC-98 - 191 04-DEC 98 OTHER TREATMENT FROM TO - 08-OCT-98 04-NOV 98 08-OCT-98 04-NOV-98 - - 08-OCT-98 04 NOV 98 08-OCT-98 04-NOV-98 -08 OCT-98 05-kOV-98 08-0CT-98 05-NOV-98 ' 08-0CT-98 05-NOW98 08-OCT-98 05-NOV-98 08-0CT-98 05-NOV-98 08 -0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 05 -NOW98 -05 NOV-98 -05 NOV-98 05-NOV-98 08-0CT-98 05-NOV-98 TREATMENT FROM TO 08-OCT-98 08-OCT-98 -08-OCT-98 08 OCT-98 08-OCT-98 08- OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 04-NOV-98 - 04-NOV-98 04-NOV 98 04-NOV- 98 05-NOV- 98 05-NOV-98 05 -NOV-98 05-NOV-98 - - 05-NOV-98 05 NOV 98 05-NOV- 98 05-NOV-98 05-NOV-98 05-NOV-98 BACK TO MAIN MORT-IND - 6 24-FEE-99 125 BACK TO MAIN NOTOX Project 242933 - SYM-IND 1 2 5-FEE-99 CLINICAL SIGNS MALES GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GRADE) ............ m T M E N T WEEKS: 1.............2.............3....,........4. 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: 3 .__________ .. .. .. .......................................:...:..:..:..:..:..:.. .. : .. : . : .. :: : : G: ............ : - : : :. . . . . . . . . . . . . . . . . . ~......................111111111 . . ................... G: ............................ ~ ~ ~ ~ ~ 1 ~ ~ 1 1 .1..1..1..1..1..1 ~ ~ 1 G:. ...1222222222221111ll11111llllllll11llll.................. G: ......11111111111113311111111111111........................ G: ......1111111111111111111111111111111111.................. I I A_N_IE_IA_c3______________-..._...-..-.-...--...-..-.-..-...-.-..-..-. ... ... ... .. .. .. .. ................................ : : SKIN / FUR / PLmmGE ALOPECIA (3) G: ..11......................:..:...:..:..:..:..:...:..:..:..:..:..:...:..:.: : (CHESp) SCABS (3) G: ................111111111111111111111111~1............... (NECK) A-N_I_M_At_4 _____________-_..-..-.-.-..-.-...-..-.-..-..-.-...-...... .. .. .. .. .. ....................... .. .. .. .. .. . . sxm/FuR/PLuMAGE ALOPECIA (3) G: ................................................... . . ...... llllll.... ................... ALOPECIA ( 3 ) G: ................................. lllllll.......... ........ (=I A..N.I.M..A.L.9......................... . . .. ... ... .................................. ..... .... .... .... .... .... ....................................... .... .... .... .... . . SmN/FuR/PLuMAGE ALOPECIA (3) G: .................... 111111111111111111111111111111111111.. (ABDOMEN) ALOPECIA (3) G: ........................................................... 1-( A-N-I-M-b-t--1_5__--_____----------....--...--...-.-.. . .. . .. .. ................ : : ........... .. .. .. .. . . . . : . : . : . : . : . . . : . : . ::: .. : VARIWS BrJusIx?S EYE' (1) 0: ...............................:..:..:..:..:..:..:...:..:..:..:..:..:..: : (EYE RIGHT) (3) G: ............................................................. (EYE RIGHT) No FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades T b , a f t e r treatment (H.Mi) A: 0.00 B: 4.00 126 .. NOTOX Project 242933 CLINICAL SIGNS, DAILY . MALES GROUP 1 (VEHICLE CONTROL) SIGN (MAx.GRADE)' (LOCATION) RECOVERY WEEKS: 1......2......3......4......5. BACK TO MAIN - SYM-IND 2 2 S -MAR-99 BVLGING EYE (1) (EYE RIGHT) DARK ( 3 ) (MERIGHT) G: 11........................... G: 11 ........................... ND RlIlTHER CLINICAL s1ms NOTED IN THIS GROUP G: Higabstdaily grades 127 NOTOX Project 242933 - - .. BACK TO MAIN - SYM-IND 3 2 5-FEB-99 CLINICAL SIGNS MALES a. GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) lwnm WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.S.6.7.1. TIME: A.N..I.M.A.L..1.7........................ . .: . .: .. . .. . ..................................................................................... .... .... sKIN/FUR/PLuMAGE ALOPECIA ( 3 ) G: ............:................;............11111........... (NMO ANIMAL 18 --_---_----L--------------------- SKIN / FUR / PLuMAGE SCABS (3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . G: .:....:...:..:....:....:...:...:.....:..:..:.....:...:...:....:...:....:...:...:...:...:...:.. : . ::::: .... l.......... : (-) ANIMAL 19 -_____________-_----____________L ... .. . .. . ......................................................................... .... ......:.................... .. . . sKIN/FuR/pMIMAGE ALOPECIA (3) G: ...................................... ll.................. (SmJLDEZ LEFT) A..N.IM..A.L..2.0........................ ... .. . .. . ..... ...................... ..... ... ... ... ... . ........:...:....:....:....:...:...:.. :.. :... :... .:....:....:...:..:.. SKIN / FUR / PLI1MAC;E KlUND (3) 0: ............................................... l.......... (GkNITAL REx;IoN) - A.N..I.M.A.L..2..1....................... . : . : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : SKIN / FUR / PLUMAGE SCABS (3) G: ........................................................... . . ..... . . . . . . . . 11......1111.. (-1 A.N.I..M.A.L..2.3........................ ... .. . .. . .. . .......... ... ... ... ... ... ... ... ... .......:.. :.. :.. .. . ... .. . .. . .. . .... .. .. . . SKIN/EwR/PLUMAGE ALOPECIA ( 3 ) G: ............................................. lllllll... ... (FORELEG LEFT) . A.N..l.M.A.t..2.5........................ . : . . .: . .: ..:...:........... .... ... .. . ... ... .......................:. .... :. :. :. :.. :.. ::: .:..:..:..:. . .: : :: sKIN/FuR/PLmAGE RED (3) . G: ........................................................... (PERIORBITAL REGION LEFT) '. A.N..I.M.A.t..2.6........................ . . . . . . . . . . . . . . . . . . . . . . . . : : : : : : ::::: : :: : : : :::::: ::: ::::::::::::: . . . . . . .. .. .. .. . . . . . . . . . . . . . . . . . . . . . SKIN / FUR / PLUMAGE SCABS (3) G: ............................ :............ ...............1 1-( A.N..I.M.A.L..2.7........................ .. .. .. ........... .. .. .. .:. .:..:...:..:..:..:..:..:..:..:..:..:..:...:..:.: : m / FUR / PLUMAGE RED ST- (3) G: :...:..:..:..:...:..:..:..:..:...:..:..:..:..:...:..:..:..:..:...:..:..:..:..:...:..: : : (PERIORBITALRXXSCN RIG") ANIMAL 29 -------------____---*------------ . .. .. . ...................................:..:..:. :. .: .: .: .................... : . : . : :.. : :.. : :.. : .:. : . :. : : : : BRwrHIm RALES (3). G: ...................................................... .I11 sKIN/FUR/PLuMAc;E ALOPECIA (3) G: ............11111111111111111111llllllllllllllllllllllllll 1-( G: Highest grades Timepfter treatment (I.& A: ) 0.00 B: 4.00 128 NOTOX Project 242933 BACK TO MAIN - . . SYM-IND 4 25 -FEB-9 9 CLINICAL SIGNS MALES GROUP 2 (TELOMER ALCOHOL) SI= (=.GRADE) ...... ............. TReATMpNT WEEKS: l....... 2.............3 4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: A..M.M..A.C..3.0........................ ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... .................................... sKIN/FuR/PLuMAGE . SLOPECIA ( 3 ) G: ............:..................111111111.................. (- RIc;IIT) No FURTHER C L I N I W SIGNS rsoTED IN THIS c;Roup , ! ' G: Kighest grades T h , a f t e r treatment ( H . a ) A: 0.00 B: 4.00 129 NOTOX Project 242933 C L m C A L SIGNS, DAILY MAtES GROUP 2 (TELOMER U O H O L ) SIGN (MAx.c;RADE) (LOCATION) RECOVERY WEEKS: 1......2......3......4......5. BACK TO MAIN - SYM-IMD 5 25-MAR- 9 9 0: .. 11111111111~1111 llll....... G: 11111........................ m FURTHER CLIMCAL SIGNS NOTED IN THIS GROUP 0 : Ki?hest daily grades 130 BACK TO MAIN NOTOX Project 242933 - SYM-IND 6 25 -FEB-99 CLINICAL SIGNS MALES GROUP 3 (TELOMER ACRYLATE) SIW (MAX.GRADE) ......... ............ TREATMENT WEEKS: 1.............2.... 3.............4. 5. mYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: c A.N.I.MA.C. .33. . . . . . . . . sKIN/FUR/Pw1MAGE . . . . . . . . . ......... ... ............................ .............. ....................................................................... . . ALOPECIA (3) 0: ............;.......,..........llllll..... ......;.......... (NECK) ALOPECIA (3) G: 11122222221111111111l111111l1l111111...................... (CHEST) ALOPECIA ( 3 ) (FORELPCI RIGHT) 0: 11122222221111111111...................................... sKIN/FUR/pLuMAGE . -ALOPECIA (3) (FLaNK LEE;T) ALOPECIA (3) DiEST) ALOPECIA ( 3 ) (FORELm RIGHT) (3) 0: 1.11.1.22.21.11.11.1.11.11.11.1.......................................................... G: ............................ llllll........................ 0: ..111111111111111111...................................... G: ........................................... 1~11111111111.. (TAIL) A..N.IM..A.L..4.5........................ .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . ..........................................:.....:.....:....:....: : : sKIN / FUR / PLUMAGE -s (3) 0: ....................................... ll...llll... ........ RED STAINING (3) 0: ........................................................... G: Highest grades T h e a f t e r treatment (H.MI) A: 0.00 B: 4.00 131 NOTOX Project 242933 CLINICAL SIGNS, DAILY . MAtES GROUP 3 (TELOMER ACRYLATE) SIGN (MAx.GRADE) (UCATION) RECOVERY WEMS: 1......2......3......4..,....5. ,..111111. BACK TO MAIN - SYM-IND 7 25-MAR-.99 G: Highest daily grades 132 BACK TO MAIN NOTOX P r o j e c t 242933 - SYM-IND B 2 5 -FEB-99 CLINICAL SIGNS MALES GROUP 4 SIGN (=.GRADE) ..... ....... ......... TREA?MENT WEEKS: 1.............2........ 3....., 4.... 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.~. TIME: A..N.I.M.A.L...5.1....................... SKIN / FUR / PLUMAGE ALOPEICIA (3) G: FORELEG RIGHT) ______ ANIMAL 56 .................... SKIN / FUR / PLUMAGE RF9 S T m ( 3 ) 0: (PERIomITALRmIaNLEFT) . .--------- G: .. . ... ... ......................:...... ... ... .. . ..:..:..:...:...:...:...:... .:.. .:.. .:.. ..:....:...:...: : : ............:........................................ l~.,. ... .... .... .................................................................... . . :. .: .: .:.: : : ..................... ::::::::::::::: lllll.........................,...... ... ... ..... .. .. .. .. .. .. .. .. .. .. .. .. .................................. .......:..:..:...................................................................... G: G: ..................................................................................................................... G: .......................................................... e: .......................................................... A..N.IM..A.L...6.2....................... ....: . : .. . . :. :. :. :. ... ... ... ... ... ... ...... .: . : . : . : . : . : . : . : . : . : . : . : VARIOUS DARK (3) G: ....................................................................................... (EYE RIGHT) A.N..I.M.A.L..6..3....................... .. .. .. ....................... .. .. .. .. ......................... .. .. .. .. ... . . SKIN / FUR / PLUMAGE SCABS (3) 0: .:..:................................................. . . . .. ............. .. llllllllllll........ . ......... (Nwo r D FURTHER CLINIw SICNS NOTED IN THIS a a J P G: Highest grades .. T i m e p f t e r treatment (H.Mi) A: 0.00 B: 4.00 133 c NOTOX Project 2 4 2 9 3 3 CLINICAL SIGNS, DAILY . WS GROUP 4 S I a (MAX.GRADE) (LOCATION) RECOVERY WEMS: 1......2... ...3 . . . ...4......5. (EYE RIGHT) VAXOVS - . E mRIGHT, INJURFS (1) DEHYDRATED ( 3 ) (EYE RIGHT) BULGINO EYE (1) (EYE RIQrrl iwuc (3) ' (EYE RIGHT) . G: G: 111111111111111111111 .22222............... 1.1.1.1.1.1... G: llllll............... ....... 0: llllll............... ....... G: 11. .......................... No FURTNFlR CLINICAL SIGNS WrED IN THIS GROUP I BACK TO MAIN - SYM-IND 9 2 5 -MAR-9 9 G: Higbest daily grades 134 BACK TO MAIN NOTOX Project 242933 SYM-IND -14 25-FEE-99 CLINICAL SIGNS MALES GROUP 5 (PFHS) S I a (MAX.ORADE) ........... ............. ... TREAlMENT wws: l... 2.......,.....3 4.......... 5. m Y S : 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: A_N_IM_AL_6_5 ______________... _... -...-.-..-...--...-.-..-...--....-.-............:..:..::..:"..'."...".."..."............................... sxIN/FUR/PLuMAGE RGD slTAmmG (3) 0: .........lllrlllllll.......:...:..:..:...:..:..:...:..:..:...:..:...:..:. : : (EAR LEET) ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ............................ ............................. 111............................ .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .......................... ........................................................................................... .. . . .. 1111 A-N_lM_A_t _79_______________..-..--..-.-.-..-.-.-.-.-.-..-.... .. .. .. .. .. ..... . . . . . . . . . . SKIN/FUR/PLuMAGE RED STAmnm (3) G: ........................................................:.......:...:..:...:...:..:...:...:..:...:.: (NMC) RED SpAININO 13) G: .......................................................... ~CEKVICALREGION) VARIOUS DRRK (3) (EYE RIGHT) G: .......................................................... No EVRTmR CLINICAL 'SIGNSNOTED IN THIS GROUP G: Highest grades T h after treatmsnt (H.Mi) A: 0 . 0 0 B: 4.00 135 NOTOX Proiect 242933 ' . .x .. a CLINICAL SIGNS, DAILY MALES GROUP 5 (PFHS) BACK TO MAIN SYH-IND -it 2 S -MAR-9 9 sKIN/FUR/PLumGE REJJ STAIMNC (3) (Nux) RED ST- (3) (CERVICAL REGION) 0: .......11.................... G: .........11~1~1~111111111111. G: 11........................... 136 BACK TO MAIN NOTOX Project 242933 ..... SYM-IND -l2 2 5 -FEB-99 C L I M C A l G SIGNS MALES ' GROTE 6 (PFOS) S I a (MAX.GRADE) .......... !rREAmENT WEEKS: l... 2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. m: 3 - A.N..I.M.A.t..0..1....................... .. . ... ... .... .... .......... .... .... .. . .. . ... ... ... ..................:... :... :.. :.. .:. .:. .:. .:....:. : . : . SXIN/FUR/FmMAGE SWELLING (3) G: ........................................ ..........111111.. 1-( A..N.IM..A.L..0.7........................ .. . ..................."............. .......................................".........................................~......~..........~... ... . . . SKIN / FUR / PLUmm ALOPECIA (3) G: .......................... llllllllllllll.....~........ ..... (CHEEK LEfi) A_N-l_MA-L_9_3_______-__-_-----...--...-..-.-..-..-....-.-....-........................................................................................... . . sKIN/FUR/PLLTMAGE SCABS (3) G: ................................. lllllll......... .......... ~NEcK) A_N-lM-A_G_9_5_________--------...--...--...-..-.-...-.-....-.........................................:...:...:...:....:...:...:...:...:....:. . .: . .: .. .:.: : : SKIN/FUR/pLuMPGE RED (3) 0: .......................................................... (HEAD) No FURTHER CLINICAL SIGNS NOTED IN THIS c;RLwp ! G: Highest grades T h , a f t e r treatment (%Mi) A: 0.00 B: 4.00 137 NOTOX Project 242933 CLINICAL SIGNS, DAILY .MALES GR.0UP 6 (PFOS) No FURTHER CLINICAL s1ms NdTED IN THIS mom BACK TO MAIN SYM-IND -13 2 5-MAR-9 9 G: Highest daily grades 138 NOTOX Project 242933 BACK TO MAIN - . . SYM-IND 14 25-FEB-9 9 CLmCAL SIGNS FEMALES GRom 1 (VEHICLE CONTROL) SIGN (MAX.GI?ADEl TREATMENT WEEKS: 1.............2.............3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TF: A.N..I.M.A.L..9.7........................ ... ... .- . . ...-.....*.....-*....*.~......:....:.. :.. .: . .: . ....:.............................................................................. . VARIOUS MIssm (1) G: ............;. ...........1111111111111111111111111111111.. (TAIL APEX) A.N..I.M.A.L..9..8....................... . .: .. . .. . ... . ............. .... ... . ... ... .. . .. . ... . .....:....:....:....:.:. :.: :... . :.. . ... . . ... ....:. :. : . VARIOUS MISSING (1) G: ......................... :::::: 1111111111111111111111111111111~. (TAIL APEX) A.N..I.M.A.L..1.0..4...................... ... ... ... ..................... .... .. .: .: .:...................*...:...:..:..:........ .....:. sxIN/FUR/puIMAGE ALOPECIA (3) ' G: ........................:...:..:..: : :~1:1:11:11:11.:..:..:..:..:..:.:..:;.:.. A..N.I.M.A.L...1.0.5...................... ... ... ... .. : ..:............ ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... . : sIou/FuR/PLLlM&GE SCABS (3) G: .............................................................................:...~ ....~ .............11..11..1.1.1 A.N..I.M.A.L..1.0..6...................... ... ... .......................................................................................... .. .. -/FuR/PLmPECIA (3) G: .......:..:..:...:.:..,.:..:..:...:..:..:..:..:..:...:..:..:..:..:...:..:..:..:..:..: : (-1 A.N..I.M.A.L..1..0.9...................... .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ... .................. sxIN/FuR/PLuMAGE ALOPECIA (31 . G: .................................. ;....................................................................... ( F U N K EUGHT) ALOPECIA- (3). G: ...11111111111........................... 111111~11........ (THIGH HIM) LEET) ALOPECIA (3) G: 11111111111111 .................... llllllllllllllll.....lll (THIGH HIM). RIGKT) A..N.IM..A.L..1..1.0...................... :... ::::::::::::::::::::::::: : :, : : : : : : : : : : : : : : : : : : : : : : : : .sxIN / FUR / PLuM?!GE ALOPECIA (3) G: ...................11.1.11.11.11.11.1.11.11.11.11.1.11.11.11.11.1.11.11.11.311.11.111111 1-( SECRETION /EzcmmIoN .SALIVATION (3) 0: ................l......................................... I------- ... ... ... ... ................................................................................................... G: .......................................................... G: .......................................................... G: .............. ............................................ G: 11112222111111111111111111................................ G: ....................................... 1111111111111111111 G: ...........111111111111111.1111111111111111111111111111111 G: Highest grades Time-after treatrent (H.Mi) A; 0.00 B: 4.00 139 BACK TO MAIN .. NOTOX P r o j e c t 242933 . .-.. SYX-IND - S 25-FEB- 99 CLINICAL SIGNS FEMALES . GROUP 1 (VEBICLE CONTROL) SIGN (MAX.GR?iDE) ALOPECIA (3) ~SHOrJLDERLEFT) ALOPECIA (3) ~HmDLEG TRFJTmNr WEEKS: 1.............2.............3.............4......*......5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.1.1.2.3.4.5.6.7.1. TIME:' G: ............................ ....... ;1111111111111111111111 G: ....................................111111111111111... .... No FURTHER CLINICAL SIGNS NOTED IN THIS QUXTP I .. G: Highest grades T h p f t e r treatmat (H.Mi) A: 0.00 E: 4.00 140 NOTOX Project 242933 - . .* CLINICAL SIGNS, DAILY . FEMALES GROTJP 1 (vEE3IcLE CONTROL) s1m (MAx.GRADE) (LOCATION) WEEKS: ..... RECOVERY 1. 2......3.. ...4.... ..5. SKIN / FUR / PLUMAGE SCABS ( 3 ) G: .. ...iiiiiiiiii BACK TO MAIN SYM-IND -36 25-MAR-33 G: .................111llllllll G: ...............ll.... ....... G: ......11111111111........... G: ......11111111111........... G: 11111111111111111........... 0: 11122222222222222........... .. G: liiqhest daily grades 141 BACK TO MAIN NOTOX Project 242933 - SYM-IND 17 25-FEB-9 9 CLINICAL SIGNS FEMAtES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.CRADE) TmAmFNr WEEKS: 1.............2.............3.............4..........:..5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: m A.N..I.M.A.L..1.1..3...................... .. .. ........................ .. .. .. .......................................................... . . SKIN/m/muMAGE A L o h I A -(3) G: ..................:................................................ . ....... 1111111112.. (m-) ' A.N..I.M.A.L..1..1.7...................... ... ... ... ... .............. ... ... ... ... . .: . .: . .: .......... .: .:.:.:.:.:.:.:.:. . :. . :. . :. : : sIcLN/FuR/PLuMAGE RED ST- (3) G: ............ll.... ........................................ (EAR RIGHT) A.N..I.M.A.L..1.1..9...................... .. .:.. .. ........................ ... ... ... ........:..:..:..:..:..:..:..:..;...:..:.: : sKIN/FuR/PLuM?GE ALOPECIA (3) G: ................................................................................. . 11111 A_N_IM_AL_1_2_1 _________-_----..--..-..--..-..--..-.-.-..-.......................... . . :. :. .: .: .: .: : : SKIN / FUR / PLUMAGE ALOPECIA (3) , G: .: .:.:..:.:..:.:..:.:..: .*...........:..:..:..:.......................................... .. .. (HEAD) mPECIA (3) (CHEEK LEFT) I G: ........................................................... G: ......................................................... 11 G: ......................... l................................ G: .................................. ll........ .............. A.N..I.M.A.L..1..2.2...................... ... .. . .. . ....................... .. ......... .. . .. .. .. ....................... .. ............ .. .. .. ... .... . . I sKIN/FUR/PLUMAGE ALOPECIA (3) G: .......................................................... (CHEEK LEFT) ! &OPECIA (3) 0: ........................................................... (CHEEK RIGHT) G: .......................................................... 0: .......................................................... A.N..I.M.A.L..1..2.3...................... .. .. .. ............... .. .. .. .. . . . . . . . . . . . . . . sKIN/FuR/pLuMAGE AtopEeIA (3) G: ..................................,..:..:..::..::..::..::..::..::.11::11::11::11::11::..::.:.:.:.:.:.:. ( q = K LEFT) A-N--R-4-A-L_1_26---_--------------------..- .. .. .. ........... .. ... ... ................... . : . : . : . : . : .... :::: : : sKIN/FUR/PLuMAGE ALOPECIA (3) G: .............. 11111111111111111........... . ............................................ (NECK) No FuRlTrm CLINICAL SIGNS Norm IN THIS GROUP G: Highest grades Time,after treatment (H.Mi) A: 0.00 B: 4.00 142 NOTOX Project 242933 - .\ CLINICAL SIGNS, DAILY . FEMALES GROUP 2 ( W M E R ALCOHOL) SIGN (MAX.GFIADE) (LOCATION) RECOVERY wws: 1......2......3......4......5. ALOPECIA (3) ALOPECIA (3) (CHEEK LEFT) ALOPECIA. (3) (CHEEK RIGHT) G: .......2222211. G: .1112222222222. G: ....1222222222. ..... G: 112222222. .... G: 1222222222. 0: .......1111111. G: .......1111111. BACK TO MAIN - SYM-IND - 1 9 25 -MAR 93 I G: Hiqhest daily grades 143 BACK TO MAIN NOTOX P r o j e c t 242933 - .-/L - SYM-IND 19 25-FEB-9 9 CLINICAL SIGNS FEMAWES GROUP 3 (TEM)MwACRYLATW SI= (MAX.GRADE) ............ T R E A m WEMS: 1.............2. 3.............4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: - A..N.X.M..U..1..2.9...................... .. .. .. .. ...:.:.:.:.:.:...: .: .: .: .: .: .: .: .: .: :. :. :. :. :. :. :. : F0.mJFf.E H U x a E D POSTURE (1) G: ....................,.:..:..:..:..:..:..1:11:111:11:1..:..:...:...:..:. : : :..:..:..:..:.. A_N_IM_At_1_31______________..._...-.-..~-....--~....-.-..-~..-.-...-:.-..,.-....:....:................................................... ... ................ .... . . . . SKIN / FUR / PLmAGE ALOPECIA (3) G: ......................... 11111111111~...................... (a==.) RED STAINING (3) G: ................................................. 1111111.. (HEAD) RED (3) G: ............................ 111111111....... .............. 1 ... (Mmc) A.N..I.M.A.L..1..3.2...................... .. .. ........... .. .. .. .. .. .. .. .. .. .. ................... . . 0: ..................................... . . .... ::::::: llll..........................,. : : : : : : : G: .......................1111111............................ G: ........................... l............................l.. G: ........................................... 111111.. ....... G: ........................ lllllllllllll............llll... .. A..N.lM..A.L..1.3.3...................... .. .. .. .. .. ...... .. .......................... ... . : . : ........... . . . ::::::::: , : #)sNRE HUNCHED WSfuRE (1) G: ................................ . . ......... lllllllllllll... ............................ A..N.I?.f.A.L..1..3.4...................... ............................. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .................................... sKxN/FuR/PLuMAGE SCABS (3) G: .................................................... 111111 1-( A.N..I.M.A.L..1..3.6....................... . : . : . :::::::::::::: ............... : . : . : . : . :::::::: ........ : .... .SKIN/FUR/SCABS (3) G: ..................................................................................... 11 (BACK) RED SpAININO ( 3 ) ' G: .................................................. l....... (HEAD) A.N..l.M.A.L..1..3.7...................... .. . ... ... .... ................. .... .... ... .. ................:...:...:...:......... .... .................. sKIN/FuR/PLuMAGE ALOPECIA (3) G: .......... 1111222211111111 :::::: lll..... ..........111111111... .. 1-( ~ A.N.I.M A.L. .13.8. . . . . . . . . . . . . . . . . ..'.. . ... . .......:.. ..:...:....:..................... .... ........... .......................".......................:.. ..:....:. ..: ..:...: : . : . WSNRE IiuKnm POSTURE (1) G: ................................................... 1111111 SKIN/FuR/PLmAGE PILOERECTION (1) ALOPECIA (3) 0: G: .............................................................................l.l.ll.l.l.l........................... 1-( G: Highest grades Time after treatment (H.Mi) A: 0.00 B: 4.00 144 BACK TO MAIN NOTOX P r o j e c t 242933 . . ..* - SYM-IND 20 25-FEE-9 9 CLINICAL SIGNS FEMAI;ES GRom 3 (TELOMWACRYLATE) SIC3 (MAX.GRADE) ............ ............ m- WEEKS: 1.............2. 3. 4.............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.~.1.2.3.4.5.6.7.1. TIME: m A_N_D__fA_L_ _1_3_9_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ .._ . .. . ..... ..... ............. ..... ..... ... ... .. . .. . ..... ................................. .... .... .. .. .. .. .............................. .. . . PoSrvRE HUNCHED pospuRE (1) G: ............:...........................................1. sKIN/FuR/pLuMAGE ALOPECIA (3) G: ............11111111111111222221222~2222222222222211111111 )-( A.N.l..M.A.G..1.4..1...................... . . : .. .. ................ .. .. .. ................ .. .. . . .. . . . : . : . : . : . : . : . : sxIN/FuR/PLIIMAGE RED SPAIMEX; ( 3 ) G: ....................................................:..:..:..:..:..:...:..:..:..:..:. : : (HEAD) ANlMA ____ G _ _ 142 __^ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ ..._ _...^ _ .... ..:..:...:.. :.. :... :...:....:..:..:..:...:...:.. :.. : .: : . : : .: : .: : .: : : ::.::.::.::.::.:...:.... sKIN/FuR/pLuMAGE RED srAs"3 (3) G: .......................... 111............................. (HEAD) A_N_XM_PiL_1_43____________--..._..-.-...--.,..-.-..-....-.-...-.-...-..-.:...:..*:..::..::..:.:.:...:....:..:.. ::.. ::. : :. :: . .. : : : .: . : .: : : : . : . : :. : : . sKIN/FuR/PLmAGE ALOPECIA (3) G: . 1111222211111111.iiiiiii iiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiii 1-( FISSURES WITH m E S S S (3) G: ....................................... ll................. (PAW HIM) RIGHT) RED STAINING ( 3 ) G: .................................. ~......lllllllllllllll.. (PAW HIM) RIGHT) No FURTHER CLINICAL SIGNS NDTED IN THIS cau3up ' G: Highest gra& Time-aftertreatment (H.Mi) A: 0.00 B: 4.00 145 .. NOTOX Project 242933 SIGN (MAX.GPADE) (LOCATION) POSNRE wws: R1.EC.O.V;E.R.2Y......3......4 ......5. G: 11111111111111. 0: 1111111.... .... G: 11111111111111. BACK TO MAIN S'tM-IND - a 2 5 -MAR-9 3 G: .......................... 11 No FUR- CLINICAL SIGNS NOTED IN THIS GROUP G: Highest daily grades 146 BACK TO MAIN NOTOX Project 242933 - a SYM-XND 25 -FEB- 99 CLINICAL SIGNS FEMALES GRom 4 ( 1 SIGN (MAX.GRADE)' ....... ......... lR?xImm WEEKS: l...... 2.............3.............4.... 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: E ANR4AL 147 -,,,-,,,,,,,,,,,,,,-,,_,,,------------ .. . ... ..... ........................... .... ... ... .... .............................. .... .... .... .......................... .. . . sKIN/NR/PLuMAGE ALOPECIA (3) G: ..................111111111111...1111111111111. ...1111111.. _________ (BACK) AN~MA1~48 __________-...--...--...-..-..-.............................. ... ..... ............................................. . . sKIN/NR/PLuMAGE SWEttIhx: (3) G: ............ : : : : : : : 1111111111111.................... : : :.:..:..:..:..:..:.. (EAR FuGHT) RED SPAINING ( 3 ) G: ...................... 111111111............. .............. (EAR RED ST- (3) G: ............ ....... 11111111111111111111111111111111111111. ... (EAR RIGHT) A_N_IM_?G_1_51___________--_:.--.:--.:-.-:-.:-.-.-.-.-.-..-....................................... ... ... ............ sKIN/FuR/PLuMAGE ALOPa2IA ( 3 ) G: ........................................................................... . . .. 11111111 (NECK) A..N.IM..A.L...1.5.2...................... WSNRE HUNCHED WSNRE (1) 0: sKIN/FuR/PLuMAGFl RED STAINING (3) G: (EAR RIGHT) A.N..IM..A.L..1..5.5...................... sKIN/FuR/pLuMAGE RED STNNim (3) G: (EAR RIGHT) A--M-M-A-G---1-5-7----------------------- sKIN/NR/puTMAGE ,---_. ALOPECIA fNM(I ' (3.) G: A u l p E c I A (3) G: mRoA!r FlDsIoN) SCALES ( 3 ) G: (THROATRDGTON) ' A.N..I.M.A.L..1.5..9...................... sKIN/FwR/PLuMAGFl A L O m I A (3) G: (NECK) SCABS (3) G: (NECK) RED STAINING (3) G: (EYE RIGHT) RED SrAINIw (3) G: (PERIORBITAL REGION RIGHT) .. . .. . .. . ... . ............. .... ... . .. . .. . .. . .. . ..... ...:...:....:... :.. :.. : . : . .: . : . .: . .:. .:..:. : . : . .............................. ::::::::<::: 1111111111111111111111111111 ...................... 111111111111111111111111111111111111 ............................. ................................................................:.....:....:....:....:.....:.....:....:. . :. . :. ... :. :. : : : 1111111111 .. .. .. .................................. .. .. .. .. .. .. .. .. .. ....................................................................................... . :.::: 111111111 ....................... 222222222222222222222222222222221111 ....................... lllllllllllll.................-...- . . . . . . . . . . . . . . . . . . .: : : : : : : : : : . . . . . . . . . . . . . .. : : : : : : : : : : : : : : : ................... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ~~~111111111111111111111111111111111111 ........................................................... .......................................................... ........................................................... -ON / McRfiIoN WATEflY DISCHARGE EX@f EY (3) G: .......................................................... (EYE RIGm No FURTHER CLXNICAC s1ms NOTED IN THIS GROUP G : Highest grades T i m e e f t e r treatment (I4.a)A: 0.00 B: 4.00 147 .. NOTOX Proiect 242933 A - ..- CLINICAL SIGNS, DAILY ' FEMAL;ES GROUP 4 ( 1 SI= (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1......2......3......4......5. G: .......................... 1. G: ...11....................... ..... .......... RED STAINms (3) G: 11 (PERIORBITAG m 1 G N XGm) llll... .... .......... ION / E c R E r I O N W A ~ Y D I X H A R G E ~ E( 3X) G: (EYE RIGHT). :...I1 ............ No FURTHER CLINICAL SIGNS M)Tp) IN THIS GROUP I BACK TO MAIN - SYM-IND 2 3 25 -M3rR-9 9 G: Highest daily grades 148 BACK TO MAIN NOTOX P r o j e c t 242933 .... - SYM-IND 14 25-FEB-9 9 CLINICAt SIGNS FEMALES GROUP 5 (PFHSI SIGN (MAX.GRADE) , TREATMENT WEEKS: 1.............2.............3.............4.......*.*...5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: W JNLMAL 162 ... ______________________^__________ ... .... .......................... ... .. .. .. ........................ ... .. .. ...................... .. .. sxIN/FuR/PL- :::::::::::::::::::::: ALOPECIA (31 G: ..1111111112222221111111112222222222111122222222~2222222.. 1-( SCABS (3) G: 1111112222111111~~1111222222111111111111111111111111111... w(NEmCK) (3) G: ........11..111112222211..................... ............. (NECK) ANIMAL 163 * -----------------------T----------- ~ .:> ... ... ... ... ... ... .. .. ..................... ::::::::::::::::::::: : : : : WSNRE HUNCHED POSTURE (1) G: ............................................................................. . 1.. A..M.M.A..G..1..6.6...................... ... ... ... .......................................................... .. .. ............... . . sKIN/FuR/ALOPECIA (3) G: ....................... . . ......... llllllllll....... . ........................... . (NECK) A..M.M..A.G...1.6.7...................... . .: ... ... .......................................... ... .. .. . . . :. :. .: .: ..:..:..: : : sKIN/FuR/PLumGE ALOPECIA (3) G: ..................................... . . .......... lllllllllllllll... ............. (NMO A..M.M.A..G..1.6..9...................... ... ... .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. sKIN/FuR/PLuMAGE ALOPECIA (3) G: ............................................... . . ..... l............ . . ................... 1-( ~oN/~ON SALIVATION (3) 0: A..M.M.A..G..1.7..3...................... sKIN/FuR/PLuMAc;E ALOPECIA (3) G: (NECK) ALOPECIA (3) G: (ABDaQIEN) ALOPECIA (3) G: (SIIOULDER FuGtpT) .......l.................................................. ... ... .... ................... ... ... ... ... ... ... ... - ... ... ... ... ... ... ... ... ... ... .... .......... . . ...................... :: 11111111111111111111U2.......r..~~~ ............11111111...........111111111111............... ................................................. 1.. ANlMAL 174 --------------------__^__________ SKIN/m/k?5 ALOPECIA (3) ... ... ... ................. ...................... ... ............................................... : : 0: ......................1 1 1 1 l 1 1 2 1 1 1 1 1 1 ~ ~ ~ ~ ~ ~ ~ G: ............1111111111222222222222222222222222111111111111 )-( A.N..l.M.A.L..1..7.5...................... .. .. .. ..................... .. .. .. ........................... ................ sKIN/FuR/PLuMAGE .. :::::::::::.::: ::::::::::: :: ALOPECIA (3) G: ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ (NECK) ALOPECIA (3) (HIUDLEG Rim) G: .......................................................... No F U R m CLINICAL SIGNS EPOTP) IN THIS GROUP G: Highest grades T h ' a f t e r treatment (I3.M) A: 0.00 8 : 4.00 149 NOTOX P r o j e c t 242933 CLINICAL SIGNS, DAILY . FEMAI;ES G R O U P 5 (PFHS) SIGN (MAx.QuIDE) (LOCATION) RECOVERY WEEKS: 1......2......3......4.,..,.5. sKIN/FUR/KuMAGE ALOPECIA (3) (NECK), G: .....11111111111111111111111 ALOPECIA (3) 1-( 0: ......1111.................. m FURTHER CLINICAL s1ms NOTED IN THIS GROUP BACK TO MAIN - SYM-IND 25 25 -MAR-99 G: Highest daLly grades 150 .. NOTOX Project 242933 - , ..~ BACK TO MAIN - S Y M - I N D 26 25-FEB-99 C L m c A L SIGNS FEMALES GROUP 6 (PPOS) SIGN (MAX.GRADE1 ........... .......... TRFATMmT WEEKS: 1.. 2.............3.............4... 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: A..N.IM..A.L..1..7.8...................... ...- ..-. : .. .:-...-...*...-....:..:....:...:....:...:...:..:..:..:....:...:...:...:...:...:...:...:.....:.....:.. : : POSNRE HUNCHED POSNRE (1) G: ....................................... llllll.............. sKIN/FUR/PLJJM?ux RED S T A r " i (3) G: ................................................... llll... 1-( A.N..I.M.A.L..1..7.9...................... ... ... .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ... ............................... POSNRE HUNCHED POSNRE (1) G: ...................................................... . ....... llllllll.... ............. 0: .............................. 111111112..222222222222222.. G: ............................... 222222222..222222222222222.. A.N..I.M.A.L..1..8.3...................... :... .:.. .... .:.. ................ . ".. ............ .......... ... .................. ... ... ......... . .. . .. . .. . . . . . SKIN / FUR / PLUMAGE SCABS (31 0: ..................................... 1111111.............. (NECK) A.N..I.M.A.L...1.8.5...................... ... ... ... ... ... ... ... ... ... ... ... ... ... ... .......................................................... sKIN/FUR/PLlmmGE RED STAININ3 ( 3 ) G: ........................................................... (HEAD) A..N.IM..A.L..1..8.6...................... ... ... ... ........................ .. .. .. .. ...........:.: f.: .: : .: : .: : .: :.: . SKIN/FUR/pLuMAGE ALOPECIA (3) 0: ............... : : : : : : : : : : : . : : : : : 111111111111111111111111................... : : : 1-( RED ST- (3) G: .......................................................... 1-( ... ... ... .. . ......... .. ..,...: : : . . : :.::.": .:..:....: ..:.:...:..:..:..:..."..:..":..:..:...:.:...: ......... G: ...................................................:..:..:..:. : .......................................................... .......................................................... ........;...................................llll.......... A..N.lE..lA.c...1.8.9...................... .. . ... ... ............... .. :. ......................... .. .. .. . :. :. :. :. .: .: .: .: .:.:.:. :. :. .: .: . .: ... .:.:.: : : SKIN / FUR / PLUMAGE. ALOPECIA (3) G: ................llll...... ................................ (ABDOMEN) .. A--M-M-A--L--1-9-0----------------------- .. . .. . ... . ..... ....................... .... .... .... ... .... ..... ....................................... ..... ........................... SKIN / FUR / PLUMAGE ALOPECIA (3) G: ......................... llllll......llllll...llllllllllll (ABDOMEN) No FURTHER CLIMCAL SIGNS NOTED IN THIS GROUP G: Highest grades T h e after treatment (H.Mi) A: 0.00 B: 4.00 151 NOTOX Project 242933 - . .-* CLINICAL SIGNS, DAILY . FEMALES GROUP 6 (PFOS) sIc;N (M?iX.GRADE) (LOCATION) RECOVERY WEEKS: 1......2.. ....3. ....4......5. G: ...11.......... -/NR/PLuMAGE RED SI- (3) (HEAD) G: ...11111111111, ... G: 1113211.....11. 0: ...111111111 G: .........lll... G: .........11111. G: 11111....................... BACK TO MAIN - SYM-IND 27 2 5 -MAR- S 9 G: Highest daily grades 152 NOTOX P r o j e c t 242933 BACK TO MAIN - BW-IND 1 17 -MAR- 9 9 1 232 219 23 5 25'2 262 .- 2 202 253 274 297 311 3 228 252 274 -300 313 4 196 214 23 0 246 255 5 209 235 258 278 288 6 221 249 270 288 304 7 222 245 269 297 316 8 197 221 244 266 280 9 2 07 236 247 274 277 10 184 206 207 229 226 11 12 2-0-7- 2--3-2 2-4-0- 2--6-4 2-7-7- 13 208 238 250 281 285 14 190 217 227 254 262 15 16 2-1-1- 233 . 243 270 275 17 224 245 265 286 299 18 23 0 261 280 3 07 329 19 219 241 259 277 288 20 209 285 2-~1 189 287 22 228 321 23 216 302 24 228 300 25 205 230- 237 265 265 26 190 209 217 237 245 27 28 197 223 . 2-3-5- 2-5-6- 2-6-0- 29 208 232 240 270 277 30 217 249 257 290 297 31 32 2-1-2- 234 2-4-5- 2--67- 2-7-3- -UP 3 (mACRrnTE) 33 220 243 261 278 296 34 191 216 234 259 273 35 224 . 242 250 2 67 277 36 211 23 3 250 269 281 37 220 243 2 63 278 290 38 211 23 6 261 282 298 39 . 206 23 1 251 280 287 40 206 232 255 276 290 41 216 245 261 292 294 42 197 220 227 257 262 43 44 2-1-0- -23--5 -2-4-6 2-7-5- 45 201 227 23 5 269 271 46 213 242 249 278 283 47 48 2-0-6- 2-2-9- 2-3-4- 2-6-7- 270 GROUP4 49 222 23 9 248 268 278 50 201 228 244 268 280 51 217 246 264 294 310 52 244 267 294 308 53 20191 236 252 278 288 1 153 281 289 301 3-0_ 9_ 332 341 354 365 341 351 3 60 377 271 282 288 298 301 308 322 330 319 334 350 356 341 349 368 381 297 304 ~~ 317 322 303 310 322 33 6 245 251 260 276 2-9-5-' -31-0- 311 323 286 299 3-0-9- -31-9- 319 354 Jii 300 ,310 343 322 307 289 269 283 292 320 2-8-9- 32~ 9 . ~ 359 322 . 307 ' 322 348 328 321 292 277 2-8-8- 298 333 2-9--7 342 3_ 84~ _ 327 320 338 363 336 333 308 287 3-0-3- 310 347 3-1-3- ,359 389 342 328 348 386 343 346 319 294 3-1-1- 317 360 3-2-0- 317 327 346 3 52 288 301 312 318 294 306 314 329 301 3l3 323 328 3 04 316 327 331 320 327 340 355 314 322 334 349 306 3 19 327 332 319 331 355 3 65 289 300 310 325 3-2-6- 3-3--7 304 3 10 326 341 304 311 325 336 298 313 324 3--3-8 294 303 308 324 3_ 01 . ~ 303 316 333 336 345 . 360 378 335 338 347 360 315 324 346 354 NOTOX Project 242933 BODYWEIGHTS (GRAM) MALeS 1 2 3 11 12 3-1-0- 3-3-9- 13 334 370 14 297 337 15 16 3-1-4- 354 17 18 19 20 21 22 23 24 25 26 27 28 2-8-9- 3-1-8- 29 289 315 30 336 385 31 32 2-9-6- 3-3-4- 33 34 35 36 37 38 39 40 41 42 43 44 45 314 352 46 312 351 47 48 310 --e 3-5--1 GRaJP4 49 50 51 52 53 ' BACK TO MAIN - BW-IND 2 17-MAR- 9 9 -----e 440 387 419 NOTOX Project 242933 BODYWEIGZITS (GRAM) . MALES BACK TO MAIN - BW-IND 3 1 -M7 AR9- 9 DAYS WEMS 1 5 8 12 15 19 22 25 28 1 1 2 2 3 3 4 4 4 c;RIxTp4 t 223 252 270 296 300 321 330 340 3 52 554 ' 206 23 2 246 263 268 290 293 306 313 56 200 223 232 249 252 267 266 278 285 57 206 2-4~ 0 . 252 ' 290 298 336 348 374 3 98 58 203 235 ' 247 270 281 309 321 334 348 59 199 228 237 266 276 299 304 314 325 --- --- --- --- --- --- --- 60 - . ,--- 61 229 264 278 317 327 359 3 69 380 397 62 215 238 253 283 288 316 321 345 359 63 205 23 0 240 ' 268 274 291 300 316 329 --- 64 -5 (Pm.5) 65 234 257 282 310 284 351 364 381 392 66 199 217 231 254 267 282 285 298 299 67 211 232 248 270 281 300 304 315 327 68 2-_14 ~ 233 249 273 283 3 01 310 322 324 69 204 225 246 2 69 273 296 295 308 3 15 70 226 257 281 305 318 34~3- 355 375 379 ~ 71 208 234 . 258 282 296 315 317 330 337 72 211 237 259 280 291 310 314 329 334 73 222 254 268 297 3 07 338 343 365 380 74 . , 189 203 201 224 219 241 245 255 256 75 --- --- --- --- --- --- --- --- 76 197 , 220 2-3-1- 2 64 266. 296 307 321 333 --- --- --- --- --- --- 77 206 2_3_3- 240 267 272 299 306 318 327 78 184 210 211 233 23 6 263 " 271 287 290 79 200 226 238 255 255 276 285 291 301 80 GROUP 6 ( N S ) 81 198 221 242 2 63 283 300 310 322 331 82 203 231 252 275 283 298 302 312 327 83 200 228 240 269 281 300 307 316 325 84 213 240 260 281 295 311 314 333 338 85 222 254 277 314 328 360 371 388 394 86 213 237 252 275 279 294 295 311 322 87 227 256 281 310 327 350 3 61 380 381 a8 211 236 252 279 ' 292 312 320 336 345 89 222 253 270 304 . 317 3 43 352 374 386 90 212 240 254 284 289 321 335 349 364 --- --- --- --- --- --- --- 91 92 204 e-- 23 4 242 276 282 318 333 351 3--6-2 93 187 204 207 229 227 242 247 2 61 265 94 220 247 250 277 276 . 299 305 320 325 95 211 237 246 275 275 295 301 312 321 155 NOTOX Project 2 4 2 9 3 3 - ..* BACK TO MAIN - BW-IND 4 1 7-MAR- 9 9 -4 ( 54 --- .. . --- 55 56 57 4~ 26~. 44-9_ ~ --- 58 367 383 59 60 --- --- 344 3-5-7- 61 3 62 406 424 437 62 326 370 383 402 63 302 342 353 370 --- ---e-- --- -----e --- ----e 453 462 473 420 428 441 383 387 396 64 GROUP5 --- 65 --- --- 6676 e-- -e- --- 68 69 70 ---e-- 71 72 73 4ia 74 275 75 3 61 e-- --- --- 76 77 356 357 360 369 314 321 324 335 --- --- 319 325 331 337 GRWP6 --- .84 . 85 --- 86 87 --- 88 89 90 -e- -e- 354 408 412 428 '429 . 340 380 384 397 404 --- ------ --- --- 91 339 377 408 92 93 245 276 298 326 94 306 343 3 62 401 95 293 329 355 384 --- --- 96 156 NOTOX Project 242933 BODY WEIGHTS (GRAM) FEMALES BACK TO MAIN - BW-IND 5 17-MAR-99 97 168 172 176 189 200 222 220 98 164 170 179 193 196 208 217 99 165 171 180 195 199 224 227 100 179 178 193 208 197 228 244 101 182 190 208 221 234 246 248 267 268 102 194 200 204 212 231 238 243 248 251 103 163 164 172 180 177 189 200 207 206 104 179 193 201 213 222 23 5 233 248 252 105 158 169 171 189 189 200 202 , 217 219 106 196 210 218 237 241 255 257 268 275 --- --- --- --- --- --- ..-- 107 172 180 185 202 200 213 206 225 232 108 e-- 109 163 . 162 166 179 181 188 195 199 204 110 169 180 185 204 200 219 215 23 8 247 111 --- --- --- --- 112 184 e-- 192 197 211 2-1-6- 2-3-0- 2-3-5- 243 2..4--3 GROUP 2 (WALCOHOL) 113 170 176 184 191 205 213 222 233 227 114 182 191 206 220 230 249 255 266 271 115 182 184 195 203 208 222 219 234 23 8 116 180 188 194 207 218 229 233 242 242 I 117 157 167 173 184 191 214 224 118 168 174 176 190 200 , 119 163 168 178 193 202 227 221 220 233 120 166 182 i9i 198 " 211 238 241 121 163 170 168 178 178 189 195 208 208 122 163 171 169 184 180 193 206 212 209 123 124 175 1-8-3- 1-8--7 2-0-0- 2-0-4- 2-1-6- 2-2-1- 2-3-6- 23 8 125 162 165 172 184 186 204 203 209 216 126 164 168 166 181 183 197 199 208 211 127 128 1-6-6- 1-8-2- 1--8-4 2-0-5- 2-0-2- 2-2-0- 2-2-3- 2-3-0- 2-3-6- ' GROUP 3 ~TELCMERACRYWLTE) 129 170 179 185 192 190 205 204 220 225 130 154 161 167 177 175 186 191 206 208 131 171 171 186 195 .201 211 212 218 222 132 175 180 193 201 209 212 221 228 231 133 1_ _53- 164 ~~ 166 176 180 191 192 203 203 134 172 181 192 197 198 206 218 234 236 135 181 192 198 216 222 237 244 250 249 136 166 17 0 183 192 184 199 204 218 215 137 157 164 167 181 188 204 212 218 214 138 167 177 176 184 '182 185 183 195 201 13 9 158 157 162 175 176 191 197 202 199 --- --- --- --- --- --- 140 -e- --- --- --- --- --- --- --- --- --- 141 154 163 169 186 186 200 197 203 206 142 160 164 166 182 ' 178 191 192 205 204 143 183 192 199 215 215 230 229 249 248 144 145 167 176 180 193 195 204 201 217 220 146 181 193 197 210 218 233 229 248 250 147 181 187 187 205 219 231 237 242 2 43 148 152 154 159 175 183 194 196 207 205 149 184 185 203 215 216 230 238 240 248 157 NOTOX Project 242933 - .-. BODYWEIGHTS (-1 FEMAweS 97 98 99 100 101 102 103 104 105 106 107 108 2-0-6- 2-2-5- 109 185 210 110 217 245 111 222 251 112 121 ' 122 123 124 125 126 127 128 GROUP 3 -( 129 130 131 132 133 134 135 136 137 138 13 9 140 141 142 143 144 GROUP4 ( 145 146 147 148 149 191 211 193 218 2-1--4 241 198 225 194 22 6 2--1-1 2-3-7- ACRYLATE) 201 23 2 177 198 182 2-1-5- 190 211 184 211 221 249 BACK TO MAIN - BW-IND 6 17-3iAR-9 9 NOTOX Project 242933 BODYWEIGHTS (GRAM) FEMALES ' - .L BACK TO MAIN - BW-IND 7 17-MAR- 9 9 . 1_ 5_ 0 ~ 151 152 153 154 155 156 ' 157 158 159 160 1_ 7_2 181 192 213 222 232 234 237 245 160 167 170 187 194 196 . 209 223 222 162 158 169 159 178 is9 188 172 186 177 202 187 206 196 221 205 225 207 177 192 194 214 211 229 232 251 255 163 169 173 191 188 199 206 217 219 --- --- --- --- --- --- --- 167 172 192 195 212 212 218 218 162 169 189 188 199 196 218 210 194 210 231 238 251 262 269 272 --- --- --- e-- GRDuP5 m3.S) 161 166 171 179 191 192 2~0~5 - 208 210 216 162 169 177 185 203 200 211 210 222 227 16~3~ 1_ 67. 179 187 182 197 204 214 214 . 164 171 181 191 194 207 206 218 223 165 157 167 180 191 202 214 220 166 173 187 195 203 216 237 234 167 166 164 180 189 200 209 211 168 185 190 197 213 224 233 250 250 169 177 189 191 204 203 223 231 23 6 170 167 174 177 187 186 200 203 217 215 171 --- --- --- --- --- --- 172 168 -1-83- 187 -2-0-4 199 214 222 232 237 --- --- --- --- --- ..-- 173 175 177 181 196 194 202 209 220 216 174 175 185 185 2 02 204 221 223 23 6 23 6 175 153 161 164 176 179 193 190 204 206 176 e-- ORDUP 6 (PFOS) 177 177 187 197 212 215 229 233 245 240 178 154 152 162 172 169 179 179 185 186 179 173 181 187 193 184 196 2 02 210 212 180 169 167 174 184 186 192 194 2-0.0. 194 181 185 182 188 194 198 204 201 205 206 182 169 178 179 190 199 208 207 217 220 183 172 172 184 196 193 202 209 215 210 184 164 166 178 191 184 198 204 206 212 185 ' 161 176 172 189 189 211 209 212 204 186 161 173 172 189 192 202 199 203 ,206 187 188 1--5-7 _15-9- 168 -a- -1-8-0 -17-8- 1--8-7 1--9-4 2-0-1- -20-3- 189 160 . 162 165 179 118 187 188 193 197 --- --- --- --- --- --- 190 157 163 167 180 179 195 200 208 215 191 192 1-6-6- 172 169 -1-8-1 180 . 186 183 192 e-- 193 159 NQTOX P r o j e c t 242933 BACK TO MAIN - BW-IND 8 17-MAR-99 150 ------ --- -e- 151 152 --- --- --- 153 189 221 228 154 23 0 257 263 155 156 2--0-1 228 -23-4- 157 205 225 233 158 197 220 214 159 252 267 268 160 --- --- --- --- -e- -e- --- --- --- 235 240 250 243 237 240 241 245 240 277 286 293 301 303 -5 wms) 161 162 ------ 163 --- 164 165 166 --e------ 167 168 ------ ------ --- --- 169 220 23 6 247 248 248 170 198 220 227 23 6 232 171 172 2-1-6- 2-4-3- 2-4-5- 2-5-8- 2-5-7- --- --- --- 173 200 22 6 225 233 226 250 174 216 249 251 256 254 273 175 188 211 217 227 229 239 176 (;Roup 6 (PFOS) 177 178 111188789120 --------- 183 184 --- --- --- 185 188 216 218 22 6 186 187 212 219 221 187 188 1--8-4 189 178 190 196 191 192 . 1--7-7 -21-4- 2-2-3- --- --- 204 209 215 219 222 227 228 239 201 209 213 . 220 160 NOTOX P r o j e c t 242933 BODY WEIGET GAIN (%) MAtES BACK TO MAIN - BWG-IND 1 17-MAR-99 1 0 -6 1 11 13 21 25 30 33 2 0 25 36 47 54 64 69 75 81 3 0 11 20 32 37 50 54 58 65 4 0 9 17 26 30 38 44 47 52 5 0 12 23 33 38 44 47 54 58 6 0 13 22 30 38 44 51 .58 61 7 0 10 21 34 42 54 57 66 72 8 0 12 24 35 42 51 54 61 63 9 0 14 19 32 34 46 so 56 62 10 0 12 l3 24 23 33 36 41 50 17 0 9 18 28 33 42 ' 47 53 60 ia 0 13 22 33 43 54 56 67 69 19 0 10 18 26 32 42 47 49 56 20 0 12 20 29 36 44 47 53 57 21 0 15 26 43 52 64 70 79 84 22 0 11 21 33 41 50 53 59 I 69 23 0 12 21 33 4_ 0 . 49 52 56 59 24 ~~ 0 9 16 25 32 35 41 46 52 25 0 12 16 29 29 41 42 50 56 26 ' 0 10 14 25 29 42 46 51 27 28 --0- -1-3- --19- 0 12 15 0 15 18 31 32 ---0 10 --16- -4-4- -4-6- -5--4 40 43 49 47 53 60 -3-6- -4-0- -4--8 33 0 lo 19 26 35 44 49 57 60 34 0 13 23 36 43 51 58 63 66 0 8 12 19 24 31 37 40 47 0 10 18 27 33 43 48 53 55 0 10 20 26 32 38 44 49 50 0 12 24 34 41 52 55 61 68 39 0 12 22 36 39 52 56 62 69 40 0 13 24 3~ 4 . 41 49 55 59 61 41 0 13 21 35 36 48 I 53 64 69 42 0 12 15 30 33 47 52 57 65 43 0 44 45 0 46 0 47 48 --0- --- --- --- 37 44 49 -5-5- 60 --- --- --- --- 17 34 35 51 54 62 7. 0. 17 31 33 43 46 53 58 14 30 31 45 52 57 64 e-- 49 0 8 12 21 25 32 36 39 46 5~ 0 . 0 13 21 33 39 50 51 57 66 51 ' 0 13 22 35 43 55 - 59 66 74 52 0 16 27 39 46 59 60 64 71 53 0 13 21 33 38 51 55 66 69 161 I .. NOTOX Project 242933 - .L BODY WEIGH" (8) M2UIES BACK TO MAIN - BWG-IND 2 17-MAR-99 11 12 -5-0- --6-4 13 61 78 14 56 77 15 16 -4-9- -6-8- --- i c;Roup2 (TELaMER ALCOHOL) 17 --a 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 -4 I I 49 50 51 52 53 .. NOTOX P r o j e c t 242933 - .. BODY WEIGET GAIN (%) MALES BACK TO MAIN - BWG-IND 3 17-MAR-99 5-~4 0 55 56 57 58 59 6_ 0 . --0- 61 0 62 0 63 0 13 21 33 35 44 48 52 58 13 19 28 30 41 42 49 52 25 26 34 33 39 43 41 45 63 69 82 93 --- --- --- --- --- --- 33 38 52 58 65 71 34 39 50 53 58 63 15 21 38 43 57 61 66 73 11 18 32 34 47 49 60 67 12 . 17 31 34 42 46 54 60 65 0 66 0 67 0 68 0 69 0 70 0 71 0 72 0 73 0 74 0 75 76 --0- 77 0 78 0 . 79 80 --0- GR3WP 6 (PFOS) 81 0 82 0 83 0 84 0 85 0 86 0 87 0 88 0 89 0 90 0 91 92 --0- 93 0 94 0 95 96 --0- 10 21 9 16 10 . 18 9 16 10 21 14 24 13 24 12 23 14 21 7 6 17 15 19 12 22 14 24 14 24 13 22 14 25 11 18 13 24 12 19 14 22 13 20 --15- 9 12 -1-2- 32 21 50 56 28 34 42 43 28 33 42 44 28 32 41 45 32 34 45 45 35 41 52 57 36 42 . 51 52 33 38 47 49 34 38 52 55 19 16 28 30 -3-5- 30 32 49 27 28 47 28 28 -4-3- 63 68 50 50 49 55 50 51 51 54 66 68 59 62 56 58 64 71 35 35 59 58 51 -e- 33 43 52 57'. 63 67 35 39 47 49 s4 ~~ 61 35 41 50 54 58 63 32 38 46 47 56 ,59 41 48 62 67 75 77 29 31 38 38 46 51 ~~ 37 44 54 59 67 68 32 38 48 52 59 64 37 43 55 59 68 74 34 36 51 58 65 72 163 NOTOX Project 242933 BODY WEIGHT GAIN (a) MALES BACK TO MAIN .. - BWG-IND 4 17 -MAR- 9 9 --- --e --- --e -e- 57 77 101 107 iia 12 1 58 58 79 81 89 89 --- --- 59 51 70 --73- 79 79 --- --- 85 98 102 107 104 78 95 99 105 106 72 87 89 93 92 --- --- e-- 73 75 90 74 76 96 63 66 77 e-- --- 68 76 78 I6.4 NOTOX Project 242933 - .." BACK TO MAIN - BWG-IND 5 17-MAR- 99 GmuP 1 (MIICLE -Id) 97 0 2 5 13 19 25 29 32 31 98 0 4 9 18 20 27 26 27 32 99 0 4 9 1-~ 8 2~1 - 2_ _9 28 38 3.8_ 100 0 -1 8 16 10 22 23 27 36 101 0 4 14 21 29 35 36 47 47 102 0 3 5 9 19 . 23 25 28 29 \ 103 0 1 6 10 9 16 23 27 26 104 0 8 12 _ 19 . 2~4- 3- _1 3-~0 3-~9 41 105 0 7 8 20 20 27 28 37 39 I 106 0 7 11 21 23 30 31 37 40 113 0 114 0 115 0 116 0 117 0 0 0 3 0 10 0 4 122 0 5 123 124 ---0 --5- 12 5 0 2 126 0 2 127 128 -,--o -1-0- GRCQIP 3 rmLmER ACRYLATE) 129 0 5 13 0 0 5 131 0 0 132 0 3 133 0 7 134 0 5 13 5 0 6 13 6 0 2 137 0 4 138 0 6 13 9 140 ---0 ----1 141 0 6 142 0 3 143 144 ---0 ---5 8 12 21 13 21 26 7 12 14 8 15 21 10 17 22 5 l3 19 9 18 24 15 19 27 3 9 9 l3 10 -1-4- -1-7- 14 15 10 12 ---2-3 -2-2- 9 13 12 a 15 14 9 14 18 10 15 19 8 15 18 12 15 15 9 19 23 10 16 11 6 15 20 5 10 9 -1-1- -1-1- 21 21 14 11 -1--7 17 -4( 145 146 147 148 149 d, 0 5 8 16 17 0 7 9 16 20 0 3. 3 13 21 0 1 5 15 20 0 1 10 17 17 165 25 31 37 34 37 40 46 49 22 20 29 31 27 29 34 34 30 27 36 43 26 29 35 32 29 31 35 43 51 29 43 45 16 . 20 28 28 18 26 30 28 -3-5- -3-6- . 29 33 27 29 -3-9- -4-2- 21 20 29 32 21 24 34 35 23 24 27 30 21 26 30 32 25 25 20 27 31 35 20 23 30 35 39 36 11 10 17 20 30 . 19 26 28 -2-6- 32 34 28 28 --- 36 36 -a- 22 20 30 32 29 27 37 38 28 31 34 34 28 29 36 35 25 29 30 35 NOTOX Project 242933 c - . .. BACK TO MAIN - BWG1-7I-NMADR- 996 97 ' 98 99 100 101 102 103 104 105 106 107 108 -2-0- -3-1- 109 13 29 110 28 45 111 112 -2-1- -3-6- Qu3vp 2 WLOMER ArXaIoII) 113 114 115 116 117 118 123 124 125 126 127 128 133 13 4 13 5 13 6 13 7 13 8 --- 139 140 -1-5- 36 141 23 37 142 15 32 --- 143 21 36 144 -e- GROUP 4 ( 145 146 147 148 149 166 --e --- 51 46 52 NOTOX Project 242933 BODY WEIGBT GAIN FEMALES - - .. BACK TO MAIN - BWG-IND 7 17-MAR-9 9 c;Roup4( . , 150 0 151 0 . 152 0 153 0 154 0 155 0 --- --- --- --- --- --- --- 156 . 157 0 158 0 159 0 --- --- --- --- 160 --- 5 12 24 29 35 36 38 42 4 6 17 21 23 31 39 39 4 10 16 15 25 27 36 39 1 1 9 12 18 24 30 31 8 10 21 19 29 31 42 44 4 6 17 15 22 26 33 34 e-- 4 3 15 17 27 27 31 31 4 4 17 16 23 21 35 30 7 8 19 23 29 35 39 40 . 161 162 163 164 168 169 170 171 172 173 . 174 175 176 0 3 8 15 16 . 23 25 27 30 0 5 9 20 ia 25 24 31 34 0 3 7 12 9 18 22 28 28 0 1 6 12 1~ 3 . 21 20 27 30 -1 6 15 22 29 34 36 40 -1 8 13 17 25 31 37 35 -2 -1 8 14 20 22 26 27 3 6 15 21 26 30 35 35 7 8 15 15 26 25 31 33 4 6 12 11 20 22 30 29 --- --- --- ..-- 9 11 21 ia 27 1 6 3 6 12 is 11 15 17 26 19 27 26 35 23 35 ---5 7 15 17 26 24 33 35 177 6 11 20 21 29 32 38 36 178 -1 5 12 10 16 16 20 21 179 5 a 12 6 l3 17 23 180 -1 3 9 10 14 15 15 181 -2 2 5 7 10 9 11 11 182 5 12 18 23 22 28 30 183 0 14 12 22 25 22 184 1 16 12 26 29 185 .o 9 17 17 32 27 186 0 7 17 19 26 28 187 188 ---0 1 ---7 --- --- 189 0 1 3 12 11 17 18 21 23 190 0 4 6 15 14 24 27 32 37 191 0 4 2 9 8 12 . 10 16 16 192 --a 167 NOTOX P r o j e c t 242933 BODY wEI(;HT GAIN (%) FEM74LES BACK TO MAIN -- BWG-SND 8 17-MAR 99 150 151 ------ 152 --- 153 20 154 30 155 156 -2-3- 157 23 158 22 159 30 160 --- CROUP5 (PFHSJ --- 161 162 --- 163 . 164 165 166 ---e----- 167 168 ------ --- 169 24 33 170 19 32 --- ---e-- 51 54 -4-6- 40 42 32 37 38 44 40 39 -e ------- -----e --e ---e-- --- --- --- -e- 44 46 49 51 48 51 55 56 --- .. --- --- 34 40 38 32 37 37 168 .. NOTOX Project 242933 - .* FOOD CONSTJMPTION (G/ANIMAL/DAY) MALES BACK TO MAIN - FC-IND 1 01-MAR-9 9 1 26 2 27 3 24 4 29 5 28 6 28 7 24 8 26 9 10 11 12 -4( 13 14 15 16 -5 17 18 19 20 2_ 6_ 28 26 27 ) 27 27 25 27 (PFHS) 26 29 24 24 26 27 29 29 25 28 31 34 29 30 .30 29 ~~ 25 27 29 30 2- .7 28 29 30 28 31 28 32 29 29 28 28 29 33 29 32 28 28 3_ 0 . 3i 26 30 25 29 28 30 34 29 28 31 29 33 31 31 28 30 30 30 32 31 30 , 29 34 31 29 32 30 28 29 31 36 29 29 32 33 31 --- --- 30 30 29 28 169 .. NOTOX Project 242933 FOOD CONSUMPTION (G/MALES /DAY) BACK TO MAIN - FC-IND 2 0 1-MAR- 9 9 170 NOTOX Project 242933 - .-. FOOD CONSIIMPTION (G/ANIMAL/DAY) FEKALES 25 18 20 21 22 26 20 21 22 23 27 20 21 23. 25 20 19 21 24 25 29 19 . 21 22 24 3301 ' 1197 . 2201 2212 2232 32 18 20 23 23 GROVP 3 ~TELOMERACRYLATE) 33 34 35 36 -4. 37 38 39 40 18 ' 1~9 . 20 22 ~~ 21 22 22 24 18 20 22 21 19 21 23 23 .: 19 20 21 23 19 21 22 23 20 22 23 24 19 22 23 23 GROUP5 (PFHS) 41 19 19 21 22 42 22 23 24 24 43 19 21 23 23 44 19 22 23 23 GRWP 6 (PFOS) 45 .22 20 20 20 46 18 19 19 19 47 17 19 19 19 48 19 20 21 20 BACK TO MAIN - FC-IND 3 0 1-MAR-99 171 NOTOX Project 242933 - _.- FOOD CONSUMPTION ( G I-/DAY) FEMALES DAYS WEEKS CAGE E--O-V--E-R-Y-- . 22-28 4 BACK TO MAIN - FC-IND 4 0 1-MAR- 9 9 172 NOTOX Project 242933 - . ..-. RELATIVE POOD CON!3Ul@l'ION (G/KG BODY WEIGH!l'/DAY) MAI;ES GROUP 1 (VEHIClJ3 CONTROL) P 110 94 89 86 2 115 102 93 90 3 107 99 99 96 4 126 116 114 101 , 1 2 3 --- 4 IS , BACK TO MAIN - RFC-IND 1 01-MAR-9 9 173 BACK TO MAIN .. NOTOX P r o j e c t 242933 - .* - R3C-IND 2 01-MAR-99 RELATIVE FOOD CONsuMpTION (G/KG BODY WEIGHT/DAY) MALES GROUP 2 (TELOMER ALCOHOL) 5 115 101 93 90 6 116 106 91 91 7 109 98 97 93 8 109 104 101 92 5 6 7 8 174 NOTOX Project 242933 R E L A T ~FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 3 (TELOMER ACRYLATE) 9 112 101 95 93 10 119 105 95 89 11 111 103 103 97 12 116 105 105 95 I 9 10 ~~ 11 12 BACK TO MAIN - RFC-IND 3 01-MAR- 99 NOTOX Project 242933 RELATIVE FOOD CONs13MpTION (G/KG BODY WEIGHT/DAY) MALFSS 103 91 90 102 93 90 15 107 105 104 100 16 111 99 98 a9 BACK TO MAIN - RFC-IND 4 O 1-MAR-99 176 NOTOX Project 242933 - . .* REL2lTIVE FOOD CONSDMPTION (G/KG BODY WEIGIET/DAY) MALES GROUP 5 (PFHS) DAYS WEmS CAGE 17 18 19 20 1-8 8.-15 15-22 22-28 ~~ i/2 2/3 3/4 4 110 101 91 88 122 ' 105 97 95 106 99 102 96 108 100 102 94 I R-E-C-O-V-E-R--Y- a DAYS 22-28 WEEKS 4 CAGE 17 18 --------- 19 20 73 ' BACK TO MAIN - RPC-IND 5 01-MAR-9 9 1-8 8-14 ' 14-19 19-22 1/2 2 2/3 3/4 177 NOTOX Project 242933 - . .* RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGE'T/DAY) MALES GROUP 6 (PFOS) . 21 120 106 94 92 22 114 102 91 87 23 111 105 104 100 24 113 103 103 99 BACK TO MAIN - RFC-IND 6 01-MAR- 99 . . ._, 178 NOTOX Project 242933 - . .* RELATIVE FOOD CONS[TMPTfON (G/KG BODY WEIGHT/DAY) FEMALES GROUP 1 (TlEEmxZ CONTROL) -- 25 106 104 98 101 26 105 102 97 94 27 103 103 104 104 28 107 107 112 109 BACK TO MAIN - R F C-IN 0 1 -M D A R - 9 7 9 179 NOTOX P r o j e c t 242933 - . .* RELATIve FOOD CONSUMPTION . (G/KG BODY wEIGHT/DAY) FI.3MALES 29 ' 30 31 32 104 102 98 97 110 108 100 102 96 105 110 101 105 106 111 108 DAYS WEEKS' CAGE. 22-28 4 BACK TO MAIN - R F C - I N D 8 01-MAR- 99 180 NOTOX P r o j e c t 242933 - . .% RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 3 (TELOMER ACRYLATE) . 33 105 100 100 101 34 117 110 107 107 35 108 110 112 105 36 110 109 112 107 BACK TO MAIN - RFC-IND 9 0 1-MAR- 9 9 181 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES DAYS WEEKS CAGE 37 38 39 40 1-8 8-15 15-22 22-28 i/2 2/3 3/4 4 104 104 98 100 110 102 102 100 115 113 112 106 104 107 106 100 BACK TO MAIN - RFC-IND 10 01-MAR-9 9 1-0 8-14 14-19. 19-22 1/2 2 2/3 3/4 182 NOTOX Project 242933 RELATIVE FOOD C0NS"I'ON (G/KG BODY WEIGHT/DAY) FEMALES G R O U P 5 (PFHS) 41 110 100 100 103 42 - 130 118 111 105 43 102 105 107 102 44 109 113 111 106 BACK TO MAIN - RFC-IND 11 01-MAR-99 183 NOTOX Project 242933 RELATIVE FOOD COJ!TSUKFTION (G/KG BODY WEIGHT/DAY) FEMAWES GROUP 6 (PFOS) DAYS WEEKS CPGE 45 46 47 48 1-8 8-15 15-22 22-28 1/2 2/3 3/4 4 125 106 101 95 104 , 97 92 92 100 100 96 93 115 111 110 102 BACK TO MAIN .. - RFC-IND 12 0 1-MAR- 9 9 1-8 8-14 14-19 19-22 1/2 2 2/3 3/4 .. 184 BACK TO MAIN - . .- NOTOX P r o j e c t 242933 TELOMER ALCOHOL ITELOMER ACRYLATE/ /PFHS/PFOS KEY-IND - 1 22 -MAR-99 KEY TO MISSING VALUES AND REMARKS HAEMATOLOGY I CLINICAL BIOCHEMISTRY AFTER 4 WEEKS Animal 1, 2, 3, 4, 56 81, 84, 97, 98, 99, 114, 129, 145, 164, 177, 179, 180, Animal 1, 33, 55, 59, 65, 72, 77, 78, 79, 89, 132, 145, 162, 168, - - - = EDTA sample clotted - - - = Citrate sample clotted Animal 62, 174 Animal 129 Animal 2, 17, 18, 19, 25, 36, 40, 49, 50, 66, 67, 71, 72, 74, 79, 87, 88, 89, 90 Animal A6 and 59 -- -1 -- = = PTT in plasma not reproducible deviating curve noted f o r PTT in plasma Serum sample showed haemolysis - - - = ALAT in serum not reproducible AFTER 2 WEEKS RECOVERY Animal 75, 169, 186 AFTER 4 WEEKS RECOVERY Animal 14, 29, 61, 78 Animal 127 - - - = Citrate sample clotted - - - = Citrate sample (partly) clotted - 1 - = ALAT in serum not reproducible .. 185 NOTOX Project 242933 - . ._ HAEM24TOLOGY AFTER 4 WEEKS MALES GROUP 1 (VEHICLE CONTROL) - - .. BACK TO MAIN - HEM-IND 1 30-MAR-99 1 ---,.-e ------ ------ 342 ----a- ------ ------ 5 9.7 0.423 56.3 1.290 22.9 12.3 6 9.2 0.406 59.4 1.347 22.7 12.2 7 7.25 9 :6 0.415 57.2 1.324 23.1 12.2 8 7.01 9.4 . 0.411 58.6 1.341 22.9 11.6 9 6.85 8.9 0.403 58.8 1.299 22.1 12.4 10 7.11 9.0 0.413 58.1 1.266 21.8 12.4 11 6.91 9.0 0.406 58.8 1.302 22.2 12.4 13 6.97 8.6 0.390 56.0 1.234 22.1 13.4 14 7.29 9.2 0.418 57.3 1.262 22.0 12.3 15 6.80 8.7 0.389 57.2 1.279 22.4 12.5 --- ------ --- ------ - --- --- --- -- 15.5 15.5 --- 15.6 14.7 16.2 17.2 5 10.9 0.105 0.010 0.000 0.025 0.860 14.2 16.1 6 17.6 0.140 0.010 0.000 0.030 0.820 15.2 16.3 7 14.1 0.075 0.000 0.000 0.030 0.895 14.3 -17.5 a 16.2 0.090 0.005 0.000 0.030 0.875 14.5 16.1 9 8.8 0.190 0.015 0.000 0.010 0.785 14.7 14.9 10 6.6 0.145 0.005 0.000 0.020 0.830 14.8 16.0 11 9.9 0.110 0.010 0.000 0.030 0. a50 14.9 . 15.7 . 13 10.9 0.145 0.000 0.000 0.025 0. 830 14.3 15.7 14 9 ..3 0.120 0.010 0.000 0.030 0 . 840 16.2 14.8 . 15 9.0 0.120 0.005 0.000 0.025 0.850 14.7 16.4 186 NOTOX P r o j e c t 242933 - .- HAEM24WLOGY AFTER4WEms MALES GROUP 2 (TELOMER ALCOHOL) - - .. BACK TO MAIN - HEM-IND 2 30-MAR-99 17 6.19 8.3 0.386 62.4 1.341 21.5 10.7 842 '*. 18 6.36 8.5 . 0.371 58.3 1.336 22.9 11.5 19 6.21 8.4 0.368 59.3 1.353 22.8 12.4 20 6.49 8.2 0.377 58.1 1.263 21.8 11.2 21 6.70 9.0 0.403 60.1 1.343 22.3 12.0 22 6.76 8.9 0.391 57.8 1.317 22.8 12.2 1216 23 7.09 9.2 24 6.93 9.2 25 6.83 8.9 0.405 57.1 0.407 58.7 0.399 58.4 1.298 1.328 1.303 22.7 22.622.3 11.1 1_2_._412.5 992 1047 1119 26 6.93 9.1 27 7.02 8.9 29 ' 7.21 9.5 30 6.51 8.7 31 6.80 8.6 0.411 59.3 1.313 22.1 12.7 897 0.404 57.5 1.268 22.0 11.7 984 0.425 58.9 1.310 22.4 12.7 1051 0.389 59.8 1.336 22.4 13.0 1140 0.391 57.5 1.265 22.0 11.7 1184 17 10.1 0.085 0.000 0.000 0.015 0.900 15.8 18 7.2 0.085 0.025 0.000 0.015 0.875 14.8 19 6.4 0.190 0.005 0.000 0.01s 0.790 15.6 20 7.3 0.155 0.015 0.000 0.040 0.790 14.5 21 10.4 0.105 0.000 0.000 0.015 0.880 14.4 22 12.3 0.115 0.015 0.000 0.040 0.830 14.5 23 8.6 0.120 0.005 0.000 0.020 0.855 15.0 24 10.9 0.080 0.010 0.000 0.010 0.900 15.4 25 10.9 ' 0.130 0.015 0.000 0.025 0.830 13.9 26 11.7 0.105 0.040 0.000 0.005 0.850 15.3 27 10.5 0.100 0.000 0.000 0.035 0.865 14.8 29 8.6 0.225 0.010 0.000 0.040 0.725 15.7 30 12.5 0.uo 0.015 0.000 0.015 0.840 15.3 31 11.9 0.130 0.005 0.000 0.015 0.850 14.5 16.0 14.9 15.4 16.4 15.8 16.9 14.6 16.6 15.5 16.5 16.2 ~~~~ 17.1 15.9 15.8 187 NOTOX P r o j e c t 242933 - .. .* MSEMATOLOGY AFTER 4 WEEKS M2YLES GROUP 3 (-MER ACRYLATE) - - BACK TO MAIN - HZM-IND 3 30-MAR- 99 H ..A.m.A.m ..L.C.G.Y ...P.A.R.A.M.F.P.E.R.S................................................................... ANplAtRBc HB HCT mcv XK MlcHc Rcw PLATELETS NUMBER T/l rmp1/1 1/1 fl fmDl nmol/l B G/1 33 6.12 8.2 34 6.50 8.5 35 6.36 8.0 36 6.43 8.2 37 6.76 9.0 38 6.83 9.2 39 6.55 8.5 40 7.16 9.4 I 41 6;49 8.7 42 6.34 8.3 43 6.83 9.0 .. 45 6.73 8.9 46 6.56 8.9 41 6.82 9.0 0.367 60.0 0.382 58.8 0.372 58.5 0.380 59.1 0.397 58.7 0.402 58.9 0.372 56.8 0.409 57.1 0.387 59.6 0.374 59.0 0.409 59.9 0.386 . 57.4 0.390 59.5 0.403 59.1 . 1.340 1.308 1.258 1.275 1.331 1.347 1.298 1.313 1.341 1.309 1.318 1.322 . 1.357 1.320 22.3 22.3 21.5 21.6 22.7 22.9 22.8 2-3_..0. 22.5 22.2 22.0 23.1 22.8 22.3 12.2 11.5 11.5 11.5. 12.7 12.3 12.5 11.6 13.1 12.4 11.8 12.1 12.1 13.4 1244 957 -1-0-6-3 93 0 1388 1354 1191 1_ 11_ 4 _. 1099 113 7 944 1268 904 1022 33 34 35 36 37 3~ 8 . 39 40 41 42 43 45 46 47 11 .8 9.9 9.1 11.2 13.4 9.4 9.6 9.3 11.2 11.8 8.9 13.8 6.7 6.8 0...1-70 0. 155 0.080 0.140 0.100 0.065 0.090 0.080 0.095 0.090 0.120 0.145 0.155 0.150 0~.0.0.5 . 0.010 0.000 0.015 0.005 0.005 0.020 0.005 0.005 0.000 0.005 0.005 0.005 0.010 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 . 0.000 0.025 0.020 0.015 0.020 0.020 0.010 0.020 0.020 0.015 0.025 0.020 0.020 0.045 0.000 0.800 0.815 0.905 0.825 0.875 0.920 0.870 0.895 0.885 0.885 0.855 0.830 0.795 0.840 --- 15.6 15.0 15.3 13.9 14.3 14.7 15.6 15.1 13.9 15.5 15.5 14.4 14.8 --- 15.5 17.4 ' 23.8 ~ 17.1 16.5 18.7 15.5 16.2 15.9 16.7 15.3 16.1 17.8 Ia 8 NOTOX Project 242933 BACK TO MAIN - HEM-IND 4 30-MAR-9 9 49 7.07 8.7 50 6.54 8.2 51 6.25 7.6 52 6.62 8.5 53 6.65 8.7 --- --- 54 7.18 9.6 55 7.13 9.4 56 57 6.42 8.7 58 6.48 8.7 ~~ 59 7 I2i 9.2 61 6.82 8.9 62 6.94 9.4 63 6.90 8.8 0.40.6. ~~ 0.371 0.358 0.387 0.384 0.429 0.418 0.386 0.389 0.418 0.402 0.418 0.395 57.4 56.7 57.3 58.5 57.7 59.7 5--8-.6 60.1 60.0 58.0 58.9 60.2 57.2 1.231 1.254 1.216 1.284 1.308 1.337 -1-.3-18 1.355 1.343 1.276 1.305 1.354 1.275 21.4 22.1 21.2 22.0 22.7 22.4 22.5 L-- 22.5 22.4 22.0. 22.1 22.5 22.3 11.7 11.5 11.7 11..7 11.9 11.7 1--2-.1 12.2 11.9 12.5 12.4 12.1 12.1 1063 1149 1030 1072 1462 874 -8-7- 6 1084 1084 860 I 1049 932 1121 49 12.4 0.100 0.010 0.000 0.025 0.865 14.9 15.7 50 15.2 0.105 0.005 0.000 0.020 0.870 14.9 15.2 51 i4.3 0.070 0.000 0.000 0.020 0.910 1_ 3 ...7 16.0 52 14.5 0.085 0.010 0.000 0.010 0.895 15.4 19.0 53. 10.6 0.065 0.010 0.000 0.015 0,910 15.2 17.4 54 8.2 0.055 0.005 0.000 ..0.020 0.920 14.1 19.5 --- 55 10.8 0.110 0.010 0.000 * 0.040 0.840 --- --- --- --- --- --- 56 13.8 26.8 57 14.1 0.095 0.000 0.000 0.035 0.870 i J .a 16.7 58 10.4 0.100 0.010 0.000 0.015 0.875 13.8 16.7 --- ..-- 59 12.2 0.075 0.010 0.000 0.030 0.885 61 9.6 0.055 0.005 0.000 0.020 0.920 14.8 19.5 --- 62 63 11.7 7.6 0.140 0.070 0.005 0.015 0.000 0.000 0.035 0.015 0.820 0.900 14.1 14.5 . 18.1 189 NOTOX P r o j e c t 242933 BACK TO MAIN .. - HEM-IND 5 30-MAR- 99 65 6.14 8.0 66 5.71 7.5 67 5.55 7.6 68 6.50 8.1 69 7.04 9.1 70 7.06 9.0 71 7.47 9 -7 72 6.40 8.4 73 6.64 8.5 74 6.72 8.7 75 6.65 8.5 77 '6.35 8.5 78. 6.95 8.6 79 6.80 9.0 0.357 0.347 0.347 0.365 0.395 0,409 0.442 0.371 0.388 0.388 0.380 0.378 0.395 0.404 58.1 60.8 62.5 56.2 56.1 57.9 59.2 58.0 58.4 57.7 57.1 59.5 56.8 59.4 1.303 1.313 1.369 1.246 . 1.293 1.275 1.299 1.312 1 * 280 1.295 1.278 1.339 1.237 1.324 22.4 11.8 21.6 11.1 21.9 . 13.1 22.2 11.0 23.0 11.1 22 .o 11.6 21.9 12.2 22.6 11.8 21.9 12.6 22.4 12.3 22.4 12.0 22.5 12.8 21.8 12.2 22.3 12.8 1017 929 946 969 1074 977 1011 873 997 943 1092 1031 221 105 65 7.3 0.175 0.005 0.000 0.030 0.790 --.. --- 66 8.1 0.255 0.020 0.000 0.020 0.705 13.9 15.9 67 11.1 0.185 0.010 0.000 0.015 0.790 14.4 15.8 68 9.2 0.125 0.000 0.000 0.035 0.840 14.1 15.8 69 7.1 0.085 0.000 0.000 0.020 0.895 14.1 17.1 70 13.6 0.170 0.010 0.000 0.025 0.795 13.5 17.4 71 72 14.2 12.1 0.140 0.115 0.000 0.010 0.000 0.000 0.040 0.025 0.820 0.850 1-4-.-7 1-8-.-1 73 13'.9 0.220 0.005 0.000 0.050 .O .725 14.7 17.3 74 8.7 0.100 0.020 0.000 . 0.025 0.855 13.7 17.4. 75 6.4 0.130 0.000 0.000 0.025 0.845 13.7 16.7 --- 77 11.0 0.105 0.015 0 9 000 0.040 0.840 -e- --- --- 78 6-0 0.160 0.005 0.000 0.005 0.830 --- --- 79 6.5 0.105 0.015 0.000 0.005 0.875 'I 90 NOTOX p r o j e c t 242933 HAEMATOLOGY AFTw4wEEKs MALES GROUP 6 (PFOS) - . .* BACK TO MAIN - HEM-IND 6 30 -MAR-9 9 81 --- --- --- 82 6.44 8.7 83 84 6-.-2-0 8.2 85 6.36 8.9 86 7.37 9.4 87 6.95 9.6 88 6.15 8.3 89 6.44 9.0 90 6.48 8.7 91 6.55 8.9 93 7.13 8.9 94 7.02 9.1 95 6.82 9.1 --- --- 0.377 58.5 0-.-3-61 . 5-8-.-2 0.395 62.1 0.429 58.2 0.414 59.6 0.367 59.7 0.395 61.3 0.382 59.0 0.390 59.5 0.389 54.6 0.409 58.3 0.398 58.4 --- --- 1.351 23.1 1-.-3-23 2-2-.-7 1.399 22.5 1.275 21.9 1.381 23.2 1.350 22.6 1.398 22.8 1.343 22.8 1.359 22.8 1.248 22.9 1.296 22.2 1.334 22.9 --- 11.3 1-1-.-5 11.2 12.7 11.3 13.7 11.5 1~2-.3 ~ 12.2 12.7 11.8 11.5 --- 1120 1-1-5- 0 1031 935 1109 1239 1019 1_ _1_141111 1127 ~ 1071 1082 81 82 83 8.4~ 85 86 87 88 8.9_ 90 91 93 94 95 --- 7.5 -10-.-2 11.3 10.8 12.3 8~. 4. ~ 13.2 14.2 10.4 7.4 10.2 12.9 --- 0.110 -0-.0- 95 0.075 0.165 0.075 0.190 0.080 0.145 0.075 0.095 0.115 0.145 --- 0.000 0-.-0-10 0.015 0.005 .0.005 0.005 0.005 0.005 0.000 0.005 0.000 0.000 --- 0.000 -0.-0-00 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 --- 0.025 -0.-0-20 0.035 0.010 0.015 0.025 0.015 0.035 0.010 0~..030 ~~ 0.025 0.035 --- 0.865 -0.-8-75 0.875 0.820 0.905 0.780 0.900 0.815 0.915 0.870 0.860 0.820 14.1 14.0 15.6 15.4 14.7 14.4 14.5' 1-4-.-1 15.6 14.7 14.6 14.5 13.8 20.8 15.2 20.0 15.0 16.9 16.1 . 19.2 1-5-.-9 17.1' 16.4 18.0 17.7 15.7 191 NOTOX Project 242933. BACK TO MAIN - HEM-IND 7 3 0-MAR- 9 9 97 ------ ------ 98 99 --- --- 100 6.50 8.9 101 6.66 9.5 102 6.80 9.0 103 6.82 9.2 104 7.02 9.6 105 6.78 8.9 106 6.79 9.2 107 6.68 8.7 109 ~~ ~ ~ 6_.5.4_ _ 8.7 110 6.63 9.0 111 6.73 9.1 ------ ------ --- --- 0.376 57.8 1.369 23.7 0.392 58.9 1.426 24.2 0.369 54.3 1.324 24.4 0.379 55.6 1.349 24.3 0.389 5_5._ 4- . 1.368 24.7 0.363 53.5 1.313 24.5 0.389 57.3 1.355 23.7 0.369 55.2 1.302 23.6 0.356 54.4 1.330 24.4 0.392 59.1 1.357 23.0 0.380 56.5 1.352 23.9 11.5 1324 11.1 1050 11.6 . 1302 11.5 1335 10.9 1078 11.9 1214 11.4 1019 11.6 996 10.5 ' 1121 999978 - --e----- --------- --- --------- --------- 111433...299 111467...217 100 ~~ ~ . 7.0 0.080 , 0.015 0.000 0.020 0.885 14.3 18.2 101 11.1 0.060 0.000 0.000 0.040 0.900 16.5 17.0 102 7.0 0.070 0.010 0.000 0.045 0.875 17.3 14.1 103 9.8 0.045 0.005 0.000 0.010 0.940. 15.6 17.5 104 9.5 0.070 0.015 0.000 0.020 0.895 15.5 16.3 105 9.0 0.060 0.010 0.000 0.015 0.915 16.0 17.0 106 6.3 0.090 0.005 0.000 0.020 0.885 15.3 16.1 107 4.9 0.130 .0.025 0.000 0.040 0.805 15.9 14.1 109 5.2 0.075 0.010 0.000 0.025 0.890 16.0 15.5 110 5.9 0.095 0.010 0.000 0.025 0.870 15.1 14.6 111 5.5 0.125 0,:ooo 0.000 0.020 0.855 15.4 14.7 192 NOTOX P r o j e c t 242933 - - , .* HAEMATOLOGY AFTER4wEEKs .. FEMALES GROUP 2 (TELOMEFl ALCOHOL) BACK TO MAIN HEM-IND 3 0 -MAR -- 9a9 115 5.59 7.7 116 6.52 8.7 117 6.28 8.9 118 6.76 9.2 119 6.51 9.0 120 6.21 8.6 121 6.00 8.3 122 6.61 8.6 123 6.29 8.2 125 6.50 8.7 126 6.55 8.8 127 6.29 8.7 0.331 0.376 0.362 0.372 0.365 0.353 0.340 0.356 0.348 0.363 0.369 0.367 59.2 57.7 57.6 55.0 56.1 56.8 56.7 53.9 55.3 55.8 . 56.3 58.3 1.377 23.3 10.8 1.334 23.1 11.2 1.417 24.6 11.2 1.361 24.7 11.2 1.382 24.7 10.7 1.385 24.4 11.2 1.383 24.4 11.0 1.301 24.2 11.8 1.304 23.6. 11.6 1.338 24.0 iZ.1 1.344 23.8 11.9 1.383 23.7 10.7 1114 970 . 1134 1463 1275 1262 1283 1097 1016 ' 1165 1095 920 113 114 -8.-9- 115 6.9 116 8.8 117 5.6 118 8.5 119 6.8 120 8.3 121 9.8 122 4.2 123 8.6 125 7.8 126 5.4 127 8.2 -0.-1-35 0.110 0.040 0 .i75 0.060 0.105 0.160 0.075 0.115 0.140 0.065 0. oa5 0.070 -0-.0-00 0.020 0.000 0.005 0.005 0.005 0.005 0.005 0.000 0.000 0.000 0.005 0.000 0--.0-00 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0-.-0-00 0.035 0.010 0.025 0.040 0.010 0.025 0.025 0.015 0.020 0.025 0.025 0.010 0.865 0.835 0.950 0.795 0.895 0.880 0.810 0.895 0.870 0.840 0.910 0.885 0.920 13.8 14.6 14.4 i4.i 15.3 15.6 15.8 15.3 15.1 15.2 14.7 15.8 15.3 15.6 16.2 16.4 14.5 15.2 15.5 16.4 15.0 15.1 13.5 15.0 14.9 13.8 14.6 14.2 NOTOX P r o j e c t 242933 4 - . .* HAEX24lVLOGY AI?= 4 WEEKS FEMALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN H E M 3 0 -IND MAR - - 9 9 9 130 7.23 9.4 131 5.98 i.5 132 6.30 8.1 133 6.69 8.9 134 6.36 8.9 1 35 6.82 9.6 136 6.48 8.7 137 6.54 9.0 138 6.70 8.8 139 6.90 8.8 141 6.83 8.9 142 6.86 9.0 143 6.22 8.6 0.408 0.327 0.360 0.372 0.370 0.388 0.359 0.379 0.362 0.373 0 ;366 0.378 0.363 56.4 54.7 57.1 55.6 58.2 56.9 55.4 58.0 54.0 54.1 53.6 55.1 58.4 1.300 1.254 1.286 1.330 . 1.399 1.408 1.343 1.376 1.313 1.275 1.303 1.312 1.383 23.0 22.9 22.5 23.9 24.1 24.7 24.2 23.7 24.3 23.6 24.3 23.8 23.7 11.3 1 2 -0 11.0 1.5 10.9 10.7 10.8 10.5 12.2 12.6 11.5 11.6 11.1 1136 1004 1089 1082 1383 1304 1180 1062 1247 1_ 06. .8. 1317 1040 1029 129 --- 1 30 5.3 1 31 6.4 1 32 133 . 6.8 5.7 1 34 8.2 1 35 6.6 1 36 5.4 137 8.2 1 38 6.4 1 39 4.2 141 13.2 142 7.9 143 4.7 --- 0.130 0.195 0.070 0.085 0.110 0.110 0.075 0.095 0...0.9.0 0.095 0.120 0.070 0.175 --- 0.005 0.005 0.000 0.000 0.000 0.015 0.005 0.030 0.020 0.015 0.005 0.015 0.005 --- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 --- 0.025 0.015 0.020 0.015 0.005 0.015 0.010 0.030 0.025 0.000 0.030 0.015 0.015 --- 0.840 0~ .7.8-5~ 0.910 0.900 0.885 0.860 0.910 0.845 0.865 0.ago 0.845 0.900 0.805 14.7 13.8 1--3-.7 14.4 15.6 15.9 15.9 16.0 14.9 15.4 15.3 15.5 14.1 17.1 15.5 16.4 16.0 16.3 14.7 14.7 U.7 14.6 15.6 194 NOTOX Project: 242933 - ...* BACK TO MAIN - H E X - I N D IiJ 30 -MAR-93 . 145 --- --- 146 6.23 8.9 147 6.64 9.0 148 6.45 8.7 149 6.66 9.6 150 6.07 0.4 151 6.86 9...4. 152 6.64. 9.2 153 6.79 8.7 154 6.99 9.4 155 6.22 8.6 157 7.33 9.4 158 6.86 8.9 159 6.33 9.0 --- --- --e 0.380 61.0 1.429 23.4 11.5 1194 0.388 58.4 . 1.355 23.2 11.5 1032 0.363 56.3 1.349 24.0 11.5 1102 0.392 58.9 1.441 24.5 11.3 1172 0.347 57.2 1.384 24.2 11.3 1276 0.385 56.1 1.370 24.4 11.4 1134 0.383 57.7 1.386 24.0 10.5 113 6 0.378 55.7 1.281 23.0 11.9 1137 0.382 54.6 1.345 24.6 11.4 1177 0 -363 58.4 1.383 23.7 11.0 1~1-6~ 9 . 0.402 54.8 1.282 23.4 11.4 1176 0.375 54.7 1.297 23.7 11.3 1332 0.368 58.1 1.422 24.5 11.0 '1165 --- 145 --- --- --- --- --- --- --- 146 10.1 0.075 0.005 0.000 0.020 0.900 14.3 16.8 147 7.1 0.080 0.005 0.000 0.015 0.900 14.8 16.6 148 9.1 0.075 0.005 0.000 0.030 0.890 13.5 16.4 149 8.3 0.090 0.010 0.000 0.010 0.890 14.5 17.5 150 4.9 0.065 0.020 0.000 0.025 0.890 14.4 17.5 151 9.8 0.075 0.005 0.000 0.035 0. 885 14.7 18.2 152 6.5 0.080 0.010 0.000 0.025 .o. 885 14.9 17.6 153 5.8 0.145 0.015 0.000 0.025 0.815 14.6 16.2 154 7.8 0..1_ 05 0.015 0.000 0_._03_0_ - 0_.8.5_0 _ . 14.4 15.5 155 4.7 0.125 0.015 0.000 0.040 0.820 i3.8 15.7 157 8.9 0.070 0.020 0.000 0.025 0.885 15.5 16.6 158 9.0 0.070 0.005 0.000 0.020 0.905 14.4 17.4 159 6.9 0.080 0.015 0.000 0.015 0.890 14.6 18.7 195 NOTOX Project 242933 BACK TO MAIN - HEM-IND 1 1 3 -M0 AR9- 9 161 5.92 8.4 0.356 60.1 1.419 23.6 10.9 1112 162 6.55 9.0 0.375 57.3 1.374 24.0 10.9 314 163 664 6-.-7-0 8-.-7- 0-.-3-66 5-4-.-6 1-.-2-99 23.8 11.8 ---. -9--75 165 6.23 8.6 0.353 56.7 1.380 24.4 11.7 1192 166 6.58 9.0 0.374 56.8 1.368 24.1 11.0 1178 167 6..65 8.8 0.360 54.1 1.323 24.4 11.9 1044 168 6.36 9.1 169 6.07 8.5 0.374 58.8 1.431 24.3 10.6 1120 0.333 5_ 4_.9- 1.400 25.5 11.3 1053 170 6.42 8.4 0.342 53.3 1.308 24.6 10.9 1066 171 6.50 9.1 , 0.381 58.6 1.400 23.9 10.7 1157 173 6.63 8.5 0.350 52.8 1.282 24.3 11.6 1019 174 5.94 8.7 175 6.02 7.8 0.359 60.4 1.343 24.8 10.6 938 0.325 54.0 1.296 24.0 12.7 1281 161 162 163 164 . 165 i66 167 168 169 170 171 173 174 175 6.3 4.8 -5-.-2 7. ..2_ 10.8 5.8 9.1 4.6 6. .. 7. 5.5 4.5 6.1 7.2 0.065 0.050 0-.-0-65 0.040 0.040 0.090 0.065 0.115 0.110 0.045 0.095 0.155 0.100 0.005 0.005 0-.-0-15 0.010 0.010 0.020 0.005 0.005 0.010 0.005 0.020 0.005 0.005 0.000 0.000 0--.0-00 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.010 0-.-0-05 0...0.4_ 5 _ 0.035 0.035 0.015 0.035 0.015 0.020 0.025 0.035 0.020 0.910 0.935 -0.-9-15 0~ .9.0~ _5_ 0.915 0.855 0.915 0.845 0.865 0.930 0.860 0.805 0.875 -13-.-7 14.0 14.3 14.1 13.9 1-5-.-8 15.9 14.4 14.2 14.4 15.1 14.0 15.8 15.7 15.1 16.4 17;9 2-2-.-1 16.0 15.5 18.1 1-5-.-9 16.8 NOTOX Project 242933 - . .- BACK TO MAIN - HEM-IND 1 2 3 -0MAR9- 9 .. .. 184 6.51 9.0 185 6.38 8.5 186 5.98 8.0 187 6.66 8.9 189 6.07 8.1 190 5.95 8.6 191 6.21 8.2 0.369 56.7 24.4 10.4 1208 0.354 55.5 1.332 24.0 12.0 1229 0.334 , 55.9 1.338 24.0 12.0 1187 0.371 55.7 1.336 24.0 11.3 1069 0.338 55.7 1.334 24.0 11.1 1423 0.361. 60.7 1.445 23.8 10.7 1033 0.333 53.6 1.320 24.6 11.3 1024 177 --- --- --- --- 178 179 180 ---4-.--5 ---0.055 e-- --0--.0--00 0----.0--00 -0.-0-10 e-- 13.3 13.6 13.9 12.9 14.8 16.7 14.7 14.6 181 7.4 0.075 0.005 0.000 0.015 0.905 14.2 16.8 182 11.8 0.080 0.005 0.000 0.020 0.895 14.7 17.7 183 11.1 0.040 0.005 0.000 0.025 0.930 14.3 20.0 184 8.3 0.085 0.005 0.000 01025 0.885 14.3 20.5 185 7.2 0.090 0.000 0.000 0.010 0.900 14.8 15.0 186 6.6 0.120 0.010 0.000 0.030. 0.840 14.4 16.2 187 6.7 0.080 0.010 0.000 0.025 0.885 14.7 15.8 189 7.9 0.070 0.000 0.000 0.020 0.910 14.1 16.4 190 8.3 0.080 0.010 0.000 0.025 0.885 14.7 16.0 191 12.1 0.075 0.005 0.000 0.015 0.905 14.5 18.1 ..I 197 NOTOX Project 242933 ImEM2lmm AFTER 2 WEEKS RECOVERY' MALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN - HEM-IND 13 3 0- M A R - 99 HAEMAMLOGY P T ----------------------------------------------------.--------------------------------------- ANIMAtRBc HB m mcv Kn M3Ic RDW NUMBER T/1 mnol/l 1/1 fl fm41 ml/l % 9 7.05 8.7 10 7.41 9.2 11 7.23 9.4 0.406 57.6 0.428 57.8 0.417 57.7 1.234 21.4 1.242 21.5 1.300 22.5 14.1 14.5 13.6 PLATELETS G/1 1293 1086 1152 9 8.1 0.150 0.010 0.000 0.030 0.810 14.6 17.4 10 6.9 0.120 0.015 0.000 0.025 0.840 14.3 15.3 11 10.5 0.065 0.015 0.000 0.025 . 0.895 15.0 15.9 198 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY' MALES GROUP 2 (TELOMER ALCOHOL) -- BACK TO MAIN - HEM-IND 14 30-MAR-9 9 25 . 6.79 8.8 26 7.06 8.9 27 7.28 8.9 0.395 58.2 0.407 57.6 0.409 56.2 1.296 1.261 1.223 22.3 . 14.2 21.9 13.9 21.8 13.0 1198 1015 103 0 - 25 14.9 0.075 0.005 0.000 0.015 0.905 15.3 15.0 26 8.6 0.210 0.000 0.000 0.030 0.760 15.6 16.1 27 12.5 0.095 0.010 0.000 0.020 . 0.875 14.7 14.8 199 NOTOX Project 242933 HAEUATOLOGY AFTW 2 WEEKS RECOVeRY' MALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN - HEM-'IND 1 5 30-MAR- 99 ' ! 41 12.1 0.090 0.010 0.000 0.025 0.875 15.3 17.1 42 U.9 0.040 0.000 0.000 0.020 0.940 14.1 15.8 43 10.2 0.l35 0.005 0.000 0.030 0.830 -15.2 15.2 200 .. NOTOX P r o j e c t 242933 - ... HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 4 ( 1 BACK TO MAIN - HEM-IND 16 3 0-MAR-99 57 6.76 8.7 58 6.94 8.8 59 7.29 8.9 0.389 0.400 0.416 57.5 57.6 57.1 1.287 1.268 '1.221 22.4 22.0 21.4 12.9 . 12.3 14.8 . 1099 1116 1024 57 14.0 58 12.2 59 10.5 0.080 0.050 0.045 0.000 0.005 0.005 0.000 0.000 0.000 . 0.030 0.020 0.020 0.890 0.925 0.930 15.0 ' 16.2 14.8 15.3 13.9 13.6 201 NOTOX P r o j e c t 242933 HAEMAToLoGy AFTER 2 WEHCS RJ3COVERY MALES GROUP 5 (PFHS) - -... BACK TO MAIN - HEM-IND 17 30 -MAR- 99 73 6.86 8.5 74 6.84 8.5 75 7.21 8.7 0.388 56.6 0.395 57.7 0.394 54.6 1.239 21.9 1.243 21.5 1.207 22.1 14.0 15.0 14.2 1028 1071 1059 --- --- 73 11.1 0.200 0.015 0.000 0.025 0.760 15.5 ' 15.3 74 6.4 0.075 0.010 0.000 0.010 0.905 14.9 15.0 75 7.9 0.125 0.010 0.000 0.020 0.845 202 NOTOX Project 2 4 2 9 3 3 HAEMATOLOGY AFTEFt 2 WEEKS RECOVERY MALES GROUP 6 (PFOS) - - .. - . .- BACK TO MAIN - HEM-IND 1 8 30-MAR-9 9 89 12.7 0.125 0.000 0.000 0.020 . 0.855 15.6 ' 16.3 90 12.8 0.155 0.005 0.000 0.020 0.820 15.9 14.6 91 9.4 0.130 0.000 0.000 0.015 0.855 14.9 13.2 203 NOTOX Project 242933 HaEMAToLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 1 (VEEICLE CONTROL) BACK TO MAIN - HEM-IND 19 30-MAR-99 105 7.02 8.8 106 7.11 9.2 107 6.93 8.9 0.399 56.8 0.414 58.2 0.398 57.4 1.254 1.294 1.284 22.1 22.2 22.4 15.8 12.9 14.7 1410 1083 1204 105 9.2 106 7.1 107 5.7 0.055 0.115 0.055 0.000 0.010 0.. 005 0,000 0,000 0.000 0.025 0.015 0.010 0.920 0.860 0.930 16.2 . 17.7 15.5 14.7 16.3 14.0 204 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER AI;COHOL) BACK TO MAIN - HEM-IND 20 3 0-MAR-99 121 6.26 8.6 122 6.63 8.4 123 6.52 8.3 0.378 60.4 1.374 22.8 14.8 1283 0.382 57.6 1.267 22.0 16.4 1024 0.373 57.2 1.273 22.3 14.4 1045 121 10.9 ~ 0.095 0.000 0.000 0.0.35_ _ 0..8_70- -1-4.6- ' 14.6 122 5.6 0.080 0.005 0.000 0.025 0.890 14.9 14.1 123 9.6 0.055 0.015 0.000 0.015 0.915 15.3 14.6 205 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 3 (TELOMW ACRYLATE) BACK TO MAIN - HEM-IND 2 1 30-,MAR-99 137 6.49 8.9 1 38 6.88 8.7 1 39 6.49 8.4 0.402 61.9 0.387 56.3 0.379 58.4 1.371 22.1 1.265 22.5 1.294 22.2 15.9 13.9 16.3 . 1053 1205 939 I 137 ii.0 1 38 6.0 139 . 4.8 . 0.060 0.050 0.085. 0.005 0.005 0.000 0.000 0.000 0.000 0.030 0.005 0.015 0.905 0.940 0.900 ' 16.1 ' 15.8 15.0 16.3 14.8 14.2 206 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALeS GROUP 4 ( 1 -- BACK TO MAIN - HEM-IND 22 - 30-MAR 9 9 153 7.9 154 6.9 155 . 5.3 0.075 0.050 0.115 0.005 0.015 0.010 0.000 0.000 0.000 0.045 0.035 0.020 0.875 0.900 0.855 14.8 . 15.1 14.5 15.7 14'.0 14.6 207 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY l?EMALES G R O U P 5 (PPHS) - ....m - - .. BACK TO MAIN - HEM-IND 23 3 0 -MAR- 9 9 169 6.22 8.2 170 6.70 8.4 171 6.91 9.2 0.357 0.376 0.416 57.4 56.1 60.2 1.318 23.0 1.254 22.3 1.331 22.1 14.0 15.8 13.9 1010 a2a 723 , 169 5.9 170 7.7 171 6.1 0.085 0.005 0.000 0.015 0.895 --- --- 0.035 0.020 0.000 0.000 0.945 14.7 16.6 0.030 0.000 0.000 0,015 0.955 14.9 14.8 208 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) - . .* BACK TO MAIN - HEM-IND 24 30-MAR-9 9 185 186 6.48 6.23 8.5 0.382 59.0 8.2 . 0.314 60.0 1.312 1.316 22.3. 21.9 15.8 15.8 1206 769 187 6.88 8.9 0.405 58.9 1.294 22.0 15.6 1127 I 185 5.3 186 9.6 187 5.4 0.115 0.100 0.085 0.000 0.000 0.005 0.000 0.000 0.000 0.025 0.020 0.035 0.860 . 0.880 -15-.-4 ' 1-3-.-7 0.875 15.5 13.7 209 NOTOX Project 242933 HAEMATOLOGY AFTW 4 WEEKS RECOVERY MALES GROUP 1 (VEBICLE CONTROL) BACK TO MAIN - HEM-IND 25 30-MAR-9 9 13 7.52 8.9 14 7.75 9.8 15 6.79 8.7 0.391 52.0 0.424 54.7 0.373 54.9 1.184 22.8 1.265 23.1 1.281 23.3 14.4 14.1 14.9 1062 981 1204 AMMAtwBc NUMBER G/1 13 13.0 14 12.5 15 11.2 SEG. 1 0.055 0.160 0.095 Eo. 1 0.010 0.010 0.000 BASO. 1 0.000 0.000 0.000 mN0. 1 0.075 0.055 0.025 LYKPH. 1 0.860 0.775 0.880 FT FTr SEC SEC 1-2-.-7 ' 1-6-.-1 13.5 15.9 . .. . 21 0 NOTOX Project 242933 HAEMAT0uX;y AFTER 4 WEKS ~ C O V W Y MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN - HEM-IND 26 3 0 -MAR- 99 . 29 7.16 9.1 30 6.88 9.2 31 6.98 8.9 0.396 55.3 0.390 56.7 0.386 55.3 1.271 1.337 1.275 23.0 23.6 23.1 12.7 12.6 13.5 917 1097 1062 =- A N I M ? l L w E c . w. BASO. mN0. LYMPH. Pr FTr G/1 1 1 1 1 SM: SEC 29 10.7 0.120 0.010 0.000 0.075 0.795 --- --- 30 10.5 0.065 0.000 0.000 0.040 0.895 13.3 14.6 31 15.8 0.100 0.000 0.000 0.050 0.850 12.7 15.7 21 1 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEERS RECOVERY MALES GROUP 3 (-MER ACRYLATE) BACK TO MAIN - HEM-IND 27 30-MAR- 99 . 46 6.70 8.9 47 7.01 9.4 0.386 57.6 1.328 23.1 13.8 960 0.395 56.3 1.341 23 .a 13.3 1024 45 15.6 0.085 0.005 0.000 0.025 0.885 13.3 . 16.1 46 8.6 0.125 0.015 ' 0.000 0.085 0.775 13.6 16.6 47 8.2 0.060 0.010 0.000 . 0.030 0.900 U.2 . 17.0 21 2 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALeS GROUP 4 ( ) -i L.._ BACK TO MAIN - HEM-IND 28 30-MAR-9 9 61' 6.84 9.2 62 ,7.18 9.5 63 7.01 9.0 0.379 55.4 0.408 56.8 0.388 55.3 1.345 24.3 1.323 23.3 1.284 23.2 .13.4 12.6 12.7 1232 1206 1100 61 10.7 62 . 9.2 63 8.2 0.055 0.055 0.070 0.005 0.010 0.000 0.000 0.000 0.000 0.030 0.015 0.015 0.910 0.920 0.915 --- . --- 13.0 15.9 14.6 17.0 21 3 NOTOX Project 242933 HAEMAT0MX;y AFTER 4 WEEKS RECOVERY MALES GROUP 5 (PF'HS) BACK TO MAIN - HEM-IND 29 30-MAR-9 9 .. 77 9.5 78 7.1 79 6.5 0.060 0.065 0.065 0.005 0.010 0.005 0.000 0.000 0.000 0.020 0.035 0.020 0.915 0.890 0.910 -14-.-5 ' -14-.-9 13.8 15.5 21 4 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEHZS ReCOVERY MALES GROUP 6 (PFOS) BACK TO MAIN - HEM-IND 3 0 30-MAR-9 9 .. 93 7.14 8.9 94 7.20 9.2 95 6.63 8.8 0.383 53.6 1.246 23.2 14.7 1083 0.406 56.4 1.278 22.7 13.2 1047 0.378 57.0 1.327 23.3 13.2 1101 93 7.5 0.040 0.005 0.000 0.030 0.925 14.2 ' 13.2 94 12.7 95 13.5 0.065 0.010 0.000 0.005 0.920 13.9 0.130 0.005 0.000 0.045 0.820 13.8 16.9 u .7 21 5 NOTOX Project 242933 . I .* HAEMATOLOGY AFTW 4 WEEKS RECOVERY FEMALES GROUP 1 (VHIICLE CONTROL) BACK TO MAIN - HEM-IND 31 3 0 -MAR-9 9 .. 109 6.76 9.2 110 . 7.09 9.4 111 6.76 9.2 0.380 56.2 0.423 59.7 0.389 57.5 1.361 1.326 1.361 24.2 22.2 23.7 12.2 13.7 12.5 911 992 1013 21 6 NOTOX Project 242933 HAEMATOLLOGY AFTER 4 WEEKS RECOVWY FEMAI;ES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN - HEM-IND 32 3 0-MAR-9 9 .. 125 8.7 126 9.9 127 7.8 0.055 0.075 0.080 0.005 0.005 0.000 0.000 0.000 0.000 0.035 0.025 0.020 . 0.905 0.895 0.900 11.9 . 14.1 12.2 ' 14.7 12.5 15.0 21 7 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEKS RECOVWY FEMAz;ES GROUP 3 (TELOMER Am-m) BACK TO MAIN - HEM-IND 3 3 - 30-MAR 99 . 141 6.87 8.9 142 7.01 9.2 143 6.48 8.8 0.381 55.5 0.398 56.8 0.388 59.9 1.295 1.312 1.358 23.4 23 -1. 22.7 13.3 13.1 13.4 1093 841 961 141 10.7 0.025 0.010 0.000 0.035 .0.830 12.2 . 14.6 142 8.9 0.055 0.010 0.000 0.010 0.925 11.9 16.7 143 3.5 0.035 0.010 0.000 0.010 0.945 11.5 15.9 NOTOX Project 242933 aAEMAToLOGY AFTER 4 WEKS ReCOVERY EZMALES GROUP4 ( 1 --.. .* - - .. BACK TO MAIN - HEM-IND 34 - 30-MAR 99 157 7.25 9.2 ,158 6.88 8.9 159 6.85 9.1 0.402 55.4 1.269 22.9 12.9 1028 0.386 56.1 1.294 23.1 12.5 1045 0.391 57.1 .1.328 23.3 12.3 969 157 6.3 158 6.5 159 5.8 0.025 0.095 0.110 0.020 0.020 0.020 0.000 0.000 0.000 0.030 0.030 0.030 0.925 0.855 0.840 12.5 ' 11.5 12.6 14.6 14.6 15.7 21 9 NOTOX Project 242933 .. .. BACK TO MAIN - HEM-IND 35 3 0-MAR-9 9 173 7.08 9.0 174 6.73 9.2 175 6.59 8.6 0.388 54.8 1.271 23.2 13.7 939 0.411 61.1 1.367 22.4 12.1 874 0.368 55.8 1.305 23.4 14.1 1120 ANIMALwBc NUMBER G/1 173 6.9 174 4.5 175 9.1 SEG. 1 0.095 0.065 0.065 m. 1 0.010 0.005 0.005 BASO. 1 0.000 0.000 0.000 Mom. 1 0.040 0.050 0.015 LYMPH. 1 0.855 0.880 0.915 FT SEC 11.8 . 12.0 11.4 FTr SM: 15.2 15.5 15.1 220 NOTOX Project 242933 HAEMAToM)(Jy AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) BACK TO MAIN - HEM-IND 36 30-MAR- 99 189 6.49 0.7 190 6.73 9.2 191 6.45 8.1 0.370 0.413 0.355 57..0 61.4 55.0 1.341 1.367 1.256 23.5 , 22.3 22.8 11.9 13.3 13.5 1062 906 1013 221 NOTOX P r o j e c t 242933 CLINICAL BIOCHEMISTRY- AFTw4wEws MAT;ES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN - CHE-IND 1 30-MAR-9 9 1 0.88 3.21 3 2 0.69 2.55 1 3 0.79 2.34 4 . 0.60 3128 5 0.66 3.30 6 0.51 2.77 7 0.67 2.76 8 0.50 2.16 2 9 0.58 2.42 3 10 0.70 2.33 3 11 0.56 3.13 2 13 0.54 2.92 2 14 0.53 2.86 2 15 0.80 3.10 3 1.58 0.75 35 6.63 6.5 1.94 0.80 29 5.67 5.9 2.63 0163 37 6.01 8.8 2.36 1.27 31 5.65 5.8 1.47 0.54 32 7.14 6.5 2.14 0.83 29 7.50 5.9 2.07 , 1.12 2~ 9 . 6.31 5.5 1.70 0_ .59_ - . 28 5.56 6.5 2;4i 0.53 38 6.70 8.1 2.53 0.88 38 6.04 8.6 1.53 0.61 33 6.93 7.7 2.04 1.05 38 6.14 7.5 1.85 0.52 30 . 4.94 5.9 1.64 1.07 39 7.31 6.9 1 54.4 29.0 25.4 1.1 2 56.6 29.6 27.0 1.1 57.5 30.4 27.1 1.1 57.8 30.4 27.4 1.1 59.9 30.4 29.5 1.0 59.1 28.9 30.2 1.0 62.5 30.2 32.3 0.9 57.5 28.5 29.0 1.0 9 55.2 28.5 26.7 1.1 10 50.8 27.7 23.1 1.2 11 53.5 30.0 23.5 1.3 13 55.9 2~ 9... 2 26.7 1.1 14 57.6 28.6 29.0 1.0 15 56.6 29.2 27.4 1.1 12.54 7.29 6.03 7.45 8.56 6.65 6.99 8.54 6.11 . 10.02 7.26 7.58 7.29 6.26 2.76 J I22 2.79 2.63 2.86 2.71 2.85 3.04 2.73 2.70 2.78 2.80 3.00 2.78 143.5 i43. i 143.1 143.1 144.8 142.3 143.3 142.8 141.6 143.1 143.3 141.3 139.8 140.6 4.70 5 -32 4.50 4.72 4.50 4.72 4.70 4.69 5.10 4.70 5.07 4.67 5.56 4.96 1 .. 2 3 4 5 6 7 8 9 10 11 13 14 15 2.46 104 2.68 101 2.53 101 2~ .55 . - ~ 102 2.63 98 2.58 97 2.64 99 2.48 100 2.47 100 2.43 100 2.47 99 2.49 102 2.58 95 2.49 97 222 NOTOX Project 242933 CL7NICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN .. - CHE-IND 2 30-MAR-9 9 ANIMAt NUMBER 17 18 19 20 21 22 23 24 25 26 27 29 30 31 ~ ......................................................................................... AtAT(GPT) ASAT(G0T) BILIT. CH0LEST.T. TRIG. CREATI"3GUCOSE VREA ukat/l ukat/l uniol/l m l / l m l / l umol/l mnol/l mnol/l 0.56 1.85 2 0.47 2.26 2 0.71 2.48 2 0.83 3.02 2 0.52 2.20 2 0.62 2.63 2 0.70 2.63 2 0.65 2.38 2 0.56 2.46 2 0.48 2.64 3 0.64 2.96 3 0.51 2.68 3 0.60 3.14 2 0.66 3.41 2 1.37 0.87 29 6.37 6.2 1.67 1.23 31 6.47 6.5 1.34 1.20 31 . 7.16 7.4 0.99 0.30 34 5.68 8.1 1.01 0.37 26 8.71 6.7 1.11 0.80 29 7.19 6.1 0.86 0.47 31 6.58 6.1 1.02 0.39 30 6.85 8.3 2.58 0.54 32 6.17 6.7 1.14 0.43 30 4.50 8.3 1.31 0.56 34 6.68 8.3 1.48 0.56 29 6.33 5.5 1.13 0.60 29 5.58 5.9 0.92 0.49 24 6.49 7.2 17 5~3.~0. 28.0 25.0 1.1 ' 6.46 2.60 143.9 4.25 18 53.8 29.3 24.5 1.2 8.92 3.04 143.3 4.65 19 54.3 31.5 22.8 1.4 7.59 2.93 143.1 4.40 20 54.9 30.8 24.1 1.3 21 54.2 27.3 26.9 1.0 6.81 2.69 143.3 4.55 8.12 2-64 143.4 5.07 22 5~ 3.~ 0 . ~ 28.8 24.2 i.2 7.04 2.90 142.6 4.64 23 52.6 27.6 25.0 1.1 6.03 2.69 142.1 4.49 24 52.6 29.5 23.1 1.3 6.84 2.76 141.4 5.14 25 53.1 27.8 25.3 1.1 . 8.21 2.65 142.3 4.60 26 53.1 28.2 24.9 1.1 11- 53 2.71 143.5 4.34 27 50.5 141 .a 4.78 29 58.0 144.8 4.69 30 51.9 142.5 4.24 31 49.1 21.7 1.3 6.01 2.76 141.9 4.51 ~~~~~ 17 2.58 101 18 2.48 100 19 2.55 102 20 2.50 103 21 2.49 94 22 2.47 99 23 2.43 101 24 2.44 99 25 2.42 101 26 2.42 102 27 2.37 101 29 2.59 io0 30 2.43 101 31 2.44 100 223 NOTOX Project 242933 I.-.- CLiXICAL BIOCHEMISTRY. AFTER 4 WEERS MALES GROUP 3 (TELOMER A(SRYLATE) BACK TO MAIN - CWE-IND 3 - 30-MAR 99 . 33 0.58 3.74 2 3_ 4_ 0...8~5- 2.81 2 35 0.64 2.72 1 36 0.62 2.46 2 37 0.52 2.00 2 38 0.45 2.31 2 5" 39 0.81 2.85 40 0 -44 1.87 41 0.48 2.46 2 42 0.50 2.27 2 43 0.73 2.62 . 2 --- 45 0.72 2.98 3 46 2.48 2 47 0.57 2.52 2 1.28 2.72 1.19 0.89 1.13 1.91 1.13 1-10 i.64 1.40 1.51 1.72 1.02 1.33 1.72 31 0.71 2-9_ 0.62 34 0.44 33 0.66 28 1.34 29 0.46 30 0.._ 68 ._ 3_ 2_ 1.U 31 0.56 31 0.43 30 0.51 34 0.43 31 0.57 , 27 6.30 5.7 5-._63- _ 6.0 6.80 7.2 5.77 7.2 6.56 5.7 6.66 7.5 6.95 6.2 7.-4-8_ 8.5 7.21 5.7 6.26 8.1 5.24 6.2 5.48 7.7 5.86 6.5 5.22 4.9 33 57.8 33.4 24.4 1.4 34 58.1 30.0 28.1 1.1 35 51.9 29.6 22.3 1.3 36 52.6 29.5 23.1 1.3 37 51.9 30.2 21.7 1.4 38 56.0 29.9 26.1 1.1 39 54.2 ~ ~.~ 28.4 25~ .8.~ 1.1 40 55.2 29.0 26.2 1.1 41 55.4 29.2 26.2 1.1 42 50.9 26.9 24.0 1.1 43 54.0 29.5 24.5 1.2 4s 52.7 29.2 23.5 1.2 46 47.6 25.8 21.8 1.2 47 56.3 29.4 26.9 1.1 NUMBER 33 34 35 36 37 38 39 40 41 42 43 45 46 47 ..................... CALCIUM CHLORIDE mnol/l m l / l 2.57 101 2.53 100 2.38 103 2.62 102 2.48 102 2.55 100 2.48 100 2.57 102 2.54 104 2.44 104 2.47 102 2.50 101 2.44 103 2.46 101 8.10 7.91 8.31 8.85 7.99 6.99 5.23 8.66 7.93 6.91 6.98 6.06 9.35 8.53 2~ .88. . 2.95 2.80 3.10 3.04 2~ .6..9. 2.90 3.22 2.92 2.79 2.55 2.90 3.01 . 2.61 .143.7 143- 5 143.2 144.5 143.1 144.0 142.6 143.7 143.6 143.0 142.4 141.7 143.0 142.6 4.81 ~ ~~ 3.92 4.81 4.32 4.95 4.85 4.66 5.14 5.30 4.69 4.58 4.81 5.13 4.09 224 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GRom4 ( 1 -- BACK TO MAIN - CHE-IND 4 3 0-MAR-9 9 49 0.68 2.49 2 50 0.47 2.41 2 51 0.63 2.09 2 52 0.52 2.30 3 53 0.69 2.85 2 54 0.53 2.16 55 0.73' 2.43 56 0.62 2.19 57 0.67 2.48 58 59 -0-.4- 9 2.58 2.52 .. 61 0.45 2.28 2 62 0.38 1.67 2 63 0.52 . 1.85 2 1.62 ~.~~ 0.56 35 1.73 0.58 34 1.75 1.22 29 1.76 0.85 31 1.65 0.51 29 1.39 0.50 37 1.33 0.73 34 2.56 0.84 32 2.37 0.66 42 2.23 0.75 32 1.54 0.60 35 1.15 0.45 38 1.98 0.50 40 1.51 ' 0.40 31 7.70 6.58 8.07 7.05 5.86 7.41 7.04 7.39 7.38 5.75 6129 6.57 4.62 4.58 7.8 6.7 . 6.4 6.8 5.7 5.6 7.1 6.9 8.6 6...8- 6.2 6.6 9.0 6.2 49 57.3 31.0 26.3 1.2 SO 51I9 30.2 21.7 1.4 51 57.0 31.0 26.0 1.2 52 56.7 32.5 24.2 1.3 53 54.2 28.8 25.4 1.1 54 52.8 28.9 23.9 1.2 55 53.5 30.4 23.1 1.3 56 53.2 28.6 24.6 1.2 57 51.0 29.3 21.7 1.4 58 56.4 31.1 . 25.3 1.2 59 53.2 28.6 24.6 1.2 61 53.3 30.5 22.8 1.3 62 51.8 28.3 ' 23.5 1.2 63 50.7 28.2 22.5 1.3 ANIMAL NUMBER 49 50 51 52 53 54 55 56 57 58 59 61 62 63 ------------------c-- CALCIUM nmol/l MLORIDE nol/l 2.45 2.43 2.52 2.55 2.48 2.41 2.45 2.36 2.54 2.57 2.37 2.47 2.56 2.45 101 104 100 98 . 102 102 102 101 103 101 99 95 104 102 8.01 7.08 8.24 10.97 7.10 9.03 8.06 8.42 8.47 7.39 6.63 6.76 7.03 8.81 2.56 2.84 2.67 2.59 2.83 2.72 2.54 2.72 3.19 2.40 2.58 2.76 3.15 2.45 143.8 143.1 141.3 142.6 143.2 143.4 142.4 143.0 142.5 142.7 143.3 143.0 143.8 144.3 4.67 4.81 4.46 4.51 4.70 4.60 4.31 4.65 4.50 4.21 4.15 4.33 4.85 4.29 225 .. NOTOX Project 242933 - i L.% CLINICAL BIOCHEMISTRY. AFTw4wEEKs MALES .. GROUP 5 (PFHS) BACK TO MAIN - CHE-IND 5 3 0-MAR-99 65 0.56 , 1.95 2 66 0.68 2.21 1 67 0.59 2.08 2 68 0.63 2.30 1 69 0.50 2.69 2 70 0.57 2.44 2 71 0.60 2.73 2 72 0.66 2.57 2 73 0.61 2.42 2 74 0.65 2.43 3 75 0.59 2.01 2 77 0.56 2.27 2 70 1.53 2.74 2 79 0.64 2.60 2 1.22 0.83 32 1.20 0.46 31 1.12 0.59 30 0.91 0.37 32 0.87 0.29 37 0.83 0.30 32 0.83 0.42 36 1.15 0.51 34 0.78 0.33 34 1.19 0.51 36 1.23 0.40 35 0.92 0.36 42 0.70 ' 0.23 50 0.68 0.37 32 9.60 7.63 6.33 7.08 7.01 8.35 6.43 7.18 5.22 6.65 7.43 4.36 4.03 6.75 ~ 7.7 7 .O 7.9 7.5 8.4 6.2 5.9 9.0 6.9 8.2 7.3 8.3 11.8 6.0 65 56.0 28.2 27.8 1.0 66 55.2 31.1 24.1 1.3 67 51.6 31.2 20.4 1.5 68 50.1 29.9 20.2 1.5 69 54.0 30.1 23.9 1.3 70 52.3 29.4 22.9 1.3 71 58.3 32.9 25.4 1.3 72 59.1 32.5 26.6 1.2 73 51.0 30.4 20.6 1.5 74 53.0 29.7 23.3 1.3 75 55 -3 3i.5 23.8 1.3 77 54.0 28.6 25.4 1.1 78 50.4 29.8 20.6 1.4 79 51.1 29.7 21.4 1.4 7.03 10.00 8.63 8.60 9.07 10.97 10.10 7.64 8.94 9.14 11.09 9.33 7.62 12.50 ~ 3.16 2.75 3.05 2.65 3.00 3.05 3.74 2.85 3.15 3.23 2.88 2.49 3.11 3.12 ~~ 143.1 143.1 143.9 143.1 144.3 142.9 144.6 142.2 144.5 143.0 143.4 142.4 142.1 143.7 ~ ~~ 4.44 4.04 4.56 4.23 4.48 4.62 5.64 4.35 4.17 5.41 4.39 4.24 4.30 4.78 66 2.45 101 67 2.56 103 68 2.40 101 69 2.54 105 70 2.51 102 71 2.64 105 72 2.53 104 73 2.54 104 74 2.56 102 75 2.44 98 77 2.40 103 78 2.49 103 79 2.48 106 225 NOTOX Project 242933 CLINICAL BIOCIIEMISTRY . AFTER4wEEKs MALES GROUP 6 (PPOS) - . - .. . BACK TO MAIN - CHE-IND 6 3 0 -MAR- 9 9 - 81 0.60 1.88 '* .. 3 82 0.58 2.80 1 83 0.49 1.70 2 1.99 0.47 30 1.09 0.91 28 0.81 0.34 38 84 0.67 2.05 85 0.49 1.67 86 0.62 2.88 87 0.60 2.06 88 0.54 2.61 3 0.96 0.48 30 89 0.62 2.78 3 ' 1.16 0.43 33 90 0.66 i.28 3 1.49 0.41 29 91 0.56 3.05 3 1.19 0.79 37 93 0.62 2.40 2 0.40 0.27 30 94 0.65 2.34 2 0.88 0.39 28 95 0.61 2.68 2 0.91 0.42 32 5.84 6.5 7.19 6.5 8.85 8.8 4.50 8.1 6.45 7.9 6.00 7.4 5.13 9.0 6.20 7.7 6.92 6.9 6.35 6.9 81 53.0 31.2 21.8 1.4 82 54.9 30.3 24.6 3.2 83 54.9 31.4 23.5 1.3 84 55.9 29.5 26.4 1.1 85 53.1 30.1 23.0 1.3 86 54.7 29.2 25.5 1.1 87 57.5 29.4 28.1 1.0 88 51i6 28.2 23.4 1.2 89 53.0 28.5 24.5 1.2 90 53.7 28.4 25.3 1.1 91 56.4 27.7 28.7 1.0 93 49.7 28.0 21.7 1.3 94 53.6 28.1 25.5 1.1 95 55.0 29.7 25.3 1.2 5.41 8.58 9.87 4.56 5.55 8.46 7.00 7.24 7.98 6.12 8.68 8.14 7.23 6.78 2.60 2.95 3 .ll 2.95 2.91 2.80 3.07 2.83 3.01 3.37 3.16 2.67 2.94 3.03 143.4 143.9 143.1 142.7 144.6 143.3 143.3 143.2 143.2 142.0 141.8 141.9 142.5 141.6 4.39 4.99 5.27 5.00 3.98 4.55 4.39 4.67 4.46 4.75 4.59 4.72 5.15 4.34 81 2.59 101 82 2.47 100 83 2.58 102 a4 2.52 106 85 2.58 105 86 2.66 101 87 2.57 106 88 2.44 102 89 2.50 104 9_ 0_ 2.57 106 91 ' 2.52 100 93 2.35 103 94 2.53 106 95 2.45 99 227 NOTOX Project 242933 C L m c A t BIO-STRY . AFTER 4 WEEKS FEMALES GROUP 1 (VEBICLE CONTROL) BACK TO MAIN - CHE-IND 7 30 -MAR- 99 97 0.69 2.74 4 ' 98 0.39 1.94 3 99 0.44 2.47 2 100 0.69 3.09 2 101 0.61 2.94 4 102 0.53 2.97 3 103 0.47 2.75 3 104 0.52 2.70 2 105 0.65 2.39 2 106 0.40 2.35 3 107 0.46 2.86 2 109 0.33 2.26 3 110 0.64 2.93 2 111 0.46 2.22 3 2.74 1.68 2.44 1.81 1.97 1.87 1.75 2.44 1.68 2.86 1.92 2.45 2.47 2.81 0.81 0.33 0.71 0.40 0.80 0.51 0.44 0.62 0.48 0;88 0.37 0.68 0.69 0.74 39 37 35 40 42 41 38 46 38 39 44 . 42 40 40 5.58 5.92 5- -..9-8 6.07 5.96 5.69 5.65 6.20 5.66 7.58 6.41 5.67 6.42 5.95 10.1 6.1 6.2 6.4 11.0 11.6 7.1 8.6 9.4 8.8 8.3 7.9 6.7 8.6 AMMAL .NUMBER ......................................................................................... PRCXEZNT. ALBUMIN GLOBULIN W G R A T I O ALP INORGPHOS SODIUM POTASSIUM g/l 911 g/l. ukat/l m l / l m l / l mnol/l 97 63.1 32.0 31.1 1.0 98 67.9 35.1 32.8 1.1 99 . 61.3 32.2 29.1 1.1 100 67.4 34.1 33.3 1.0 101 67.1 34.4 32.7 1.1 102 61.5 33.4 28.1 1.2 103 70.1 36.4 33.7 1.1 104 70.9 34.9 36.0 1.0 105 67.3 34.0 33.3 1.0 106 ~~ 65.5 34.6 30.9 1.1 107 64.8 32.6 32.2 1.0 109 60.8 32.1 28.7 1.1 110 69.8 36.1 33.7 1.1 111 64.7 34.2 30.5 1.1 3.33 4.34 5.17 5.53 4.09 ' 4.41 3.91 5.50 4.21 3.87 4.50 4.79 4.35 4.39 2.87 2.50 2.67 2.61 2.68 2.93 2.27 2.52 2.87 2.68 2 .SO 2.12 2.37 2.49 142.4 144.1 146.1 11g.8 143.7 142.1 141.6 141.7 141.0 ,141.4 142.0 141.4 142.7 141.1 4.85 3.41 3.96 4.59 4.03 4.78 4.08 4.18 4.13 4.09 3.77 3.91 3.87 4.21 g.97 2.72 103 2.60 101 2.59 100 100 2.47 98 101 2.66 93 102 2.52 98 103 2.59 98 104 2.58 96 105 2.59 93 106 2.66 94 107 2.45 96 109 2.37 97 110 2.52 96 111 2.58 96 228 NOTOX Project 242933 CLINICAL BIOCEEMISTRY . AFTER 4 WEEKS FEMALES GFtOlB 2 (TELOMER ALCOHOL) BACK TO MAIN - CHE-IND 8 30-MAR-9 9 113 0.60. 1.59 3 114 0.48 2.06 3 115 0.56 2.28 3 116 0.43 2.22 3 117 0.39 1.98 2 118 0.47 3.45 3 119 0.66 3.15 3 120 0.35 2.12 2 0.51 2.34 2 0.38 2.91 2 0.39 2.15 2 0.37 . 2.76 2 0.45 2.40 2 0.47 1.75 2 2.41 0.47 33 2.66 0.52 34 2.22 1.07 36 2.56 0.48 44 1.70 0.47 35 2.14 0.60 42 2.86 0.69 40 2.14 0.46 36 2.24 0.52 4~1 - 2.15 0.60 39 1.46 0.42 36 2.57 0.63 37 1.85 0.31 39 2.69 0.69 44 5.30 5.86 5.63 6.82 6.08 6.00 5.66 5.90 7...4~ 0 . 5.79 5.70 5.76 5.38 5.29 7.4 7.1 6.9 10.1 7.2 11.7 9.1 8.6 9.5 7.4 6.3 8.5 8.6 8.7 113 66.6 34.2 32.4 1.1 114 66.1 30.8 35.3 0.9 115 68.2 34.1 34.1 1.0 116 65.6 34.0 31.6 1.1 117 61.3 32.5 28.8 1.1 118 67.0 35.0 32.0 1.1 119 67.9 33.7 34.2 1.0 120 63.6 33.6 30.0 1.1 121 59.6 32.5 27.1 1.2 122 68.8 37.0 31.8 1.2 123 64.6 34.6 30.0 1.2 125 63.3 34.2 29.1 1.2 126 63.2 33.9 29.3 1.2 127 61.3 32.9 28.4 1.2 2.57 4.21 3.54 3.67 4.13 3.19 5.23 4.08 5.43 5.30 3.19 2.78 4.25 5.05 2.57 141.5 2.69 142.5 2.43 143.3 2.63 143.1 2.28 143.0 2.70 142.2 2.69 140.9 2.71 142.5 2.60 143.2 2.39 143.5 2.48 142.0 2.57 , 142.4 2.41 142.7 2.20 143.7 4.20 4.43 4.08 3.91 3.79 4.79 4.55 4.14 4.44 4.19 3.79 4.04 4.10 3.49 113 2.66 102 114 2.60 99 115 2.67 102 116 2.63 99 117 2.50 100 iie 2.57 101 119 2.63 97 120 2.60 96 121 2.57 96 122 2.65 98 123 2.57 96 125 2.65 98 126 2.54 97 127 2.51 99 229 NOTOX Project 242933 - .-..a CLINICAL BIOCHEMISTRY . AFTw4wEKs EZMAlXS GROUP 3 (TELOMW ACRYLATE) .- . . BACK TO MAIN - CHE-IND 9 - 3 0 -MAR 99 129 0.30 1.66 2 130 0.57 2.18 3 131 0.47 2.08 2 132 0.49 1.97 2 133 0.84 2.32 3 134 0.76 2.15 3 13 5 0.66 2.60 3 136 0.60 2.45 3 137 0.43 1.99 2 138 0.44 2.82 2 139 0.47 1.73 3 141 0.55 2.47 3 142 0.58 2.12 3 143 0.51 2.82 3 1.74 0.51 32 1.83 0.49 37 1.76 0.67 34 2.50 0.56 37 2.88 0.74 37 2.53 0.81 38 2.17 0.80 44 1.68 0.62 38 1.41 0.41 45 2.03 0.60 40 1.99 0.53 40 2.14 0.38 41 2.18 0.40 38 2.39 0.59 43 5.27 5.23 6.67 s.ia 6.32 5.83 5.62 6.15 5.83 6.89 6.20 6.08 5.98 7.96 5.5 8.4 7.0 9.4 10.4 9.7 9.5 8.5 11.4 8.4 7.3 11.3 7.0 9.6 129 130 131 132 . 133 134 135 136 137 138 l39 141 142 143 64.4 66.2 68.0 62.0 69.5 62.8 65.6 66.7 60.2 65.8 64.2 66.9 66.4 66.8 32.3 34.0 35.3 33.5 3-4~ .9 . - 32.0 33.5 34.5 31.2 35.0 - 36.2 33.8 35.5 36.4 32.1 32.2 32.7 28.5 34.7 30.8 32.1 32.2 29.0 30.8 28.0 33.1 30.9 30.4 1.0 1.1 1.1 1.2 1.0 1.0 . 1.0 1.1 1.1 1.1 1.3 1.0 1.1 1.2 3.01 4.48 3. .. 0.2 ~ 5.49 3.20 5.05 5.59 3.32 5.91 5.72 3.94 6.27 3.43 4.90 2.43 2.58 2.40 2.73 2.41 2.72 2~.6..9. 2.38 2.29 2.45 2.14 2.64 2.55 2.10 ~ 143.0 143.2 143.1 142.7 142.8 140.9 140.9 143.4 142.6 144.3 143.0 142.0 143.1 140. a ~ 3.81 4.28 3.64 3.97 ' 3.89 4.31 4.41 4.04 4.44 3.63 4.01' 4.59 ' 4.02 4.56 129 2.60 99 13 0 2.60 99 131 2.54 100 13 2 2.57 99 133 2.72 98 134 2-55 96 13 5 2.64 9.6. 13 6 2.61 96 137 2.52 100 13 8 2.60 98 139 2.65 96 141 2.63 102 142 2.64 95 143 2.54 100 230 NOTOX Project 242933 CLIXICAL BIOCHEMISTRY' AFTw4- FEMALES GROUP 4 ( 1 BACK TO MAIN - CHE-IND 10 3 0-MAR- 99 . ,145 0.41 2.21 2 146 0.26 2.15 3 147 0.49 2.27 3 148 0.57 1.80 2 149 0.44 1.74 3 1.73 0.65 37 2.25 0.53 42 2.61 0.46 41 2.38 0.56 34 2.20 0.73 36 5.98 6.7 6.02 8.2 5.37 8.5 5.79 7.1 6.92 9.8 150 . 0.61 2.67 5 2.70 0.47 42 5.40 10.6 151 0.56 2.34 4 2.80 0.87 43 6.00 9.9 152 0.55 2.21 '4 2.08 0.44 45 5.98 10.6 , 153 0.49 2.67 2 154 0.47 2.18 2 2.04 0.56 43 2.55 0.56 37 5.78 8.9 6.69 5.5 155 0.54 2.64 2 2.14 0.42 43 6.21 10.9 157 0.49 2.99 3 2.83 0.45 44 5.68 9.2 158 0.68 1.97 3 2.05 0.48 40 5.39 8.4 159 0.74 2.31 3 2.09 0.62 46 6.74 11.3 A"AL NUMBER 145 146 147 148 149 150 151 152 153 154 155 157 ......................................................................................... PKYE.INT. ALBUMIN GLOBULIN A/GRATIO ALP INORGPHOS SODIUM POTASSIUM g/l g/l g/l ukat/l mnol/l m l / l m l / l 67.8 35.0 32.8 1.1 67.5 33.0 34.5 1.0 64.8 30.1 34.7 0.9 61.7 32.0 29.7 1.1 67.7 34.5 33.2 1.0 67.2 34.1 33.1 1.0 65.3 34.7 30.6 1.1 70.3 37.6 32.7 1.1 70.7 38.3 32.4 1.2 60.9 32.9 28.0 1.2 65.0 32.4 32.6 1.0 6..7..6. 32.7 34.9 0.9 67.0 33.6 33.4 1.0 63.3 32.8 30.5 1.1 3.53 2.18 143.7 3.82 5.95 2.34 143.0 4.06 4.82 2.30 141.9 4.45 4.31 2.46 140.4 3.91 3.45 2.60 142.7 4.47 3.68 2.61 142.0 4.31 3.07 2.90 141.6 4.28 3.66 2.29 143.4 3.82 3.72 2.25 141.9 4.30 5.74 2.33 141.9 4.24 4.54 2.47 143.2 4.46 4.17 2.28 140.6 4.37 5.22 2.50 141.5 3.93 4.36 2.30 140.8 4.30 145 2.57 100 146 2.63 103 147 2.52 99 148 2.58 98 149 2.64 95 150 2.63 98 151 2.66 98 152 2.64 97 153 2.60 101 154 2.57 99 155 2.55 102 157 2.58 100 I58 2.68 100 159 2.50 99 231 ' NOTOX Project 242933 CLIJUCAL BIOCHEMISTRY AFTER4wEEKs FEMALES GROUP 5 (PFHS) BACK TO MAIN - CHE-IND 11 - 30-MAR 99 161 0.52 1.77 3 162 0.50 3.54 3 163 0.78 2.18 2 1-~64165 0.53 0.57 1.61 2.01 3 3 166 0.49 3.77 2 167 0.57 2.27 3 168 0.62 2.86 2 169 0.65 2.49 2 170 0.59 1.75 2 171 0.60 1.61 2 173 0.40 2.79 2 174 0.62 2.88 2 175 0.53 2.73 3 1.93 1.00 38 6.34 7.7 2.16 .0.52 40 5.01 7.2 1.64 0.48 3.8. 4.93 9.6 1.80 0.32 35 ' 6.21 7.9 2.23 0.55 35 5.64 7.8 1.76 0.45 37 5.33 7.6 1.46 0.49 39 6.02 9.6 1.94 0.55 45 5.96 9.1 1.59 0.59 44 5.77 8.5 2.06 0.44 40 5.92 8.2 2.16 0.44 42 7.44 7.8 1.32 0.38 44 6.18 8.6 1.66 0.33 44 6.23 9.2 1.68 0.53 42 7.30 9.9 161 162 163 1_ 64. 165 166 167 168 169 170 171 173 174 175 74.6 66.1 71.1 6~ 6...563.9 60.5 63.1 66.2 63.2 62.4 70.0 64.5 60.1 63.5 35.6 32.4 35.0 34~ .7. ~ 33.3 31.3 33.8 33.9 32.2 ~~ ~ 33.2 35.8 33.8 31.3 35.4 39.0 33.7 36.1 31.8 30.6 29.2 29.3 32.3 31.0 29.2 34.2 30.7 28.8 28.1 0.9 . 1.0 1.0 1.1 1.1 1.1 1.2 1.0 1.0 1.1 1.0 1.1 1.1 1.3 3.89 6.82 4-..6~3- 3.95 3.02 4.31 3.49 4.11 5.07 3.03 3.41 2.49 5.06 3.54 2.16 2.28 2.42 2.32 2.81 2.79 2.64 2.56 2.39 2.21 2.35 2.47 2.36 2.22 141.3 141.8 141.5 142.2 141.9 ,143.1 141.9 142.3 140.8 142.7 142.8 141.0 141.1 141.0 3.90 3.86 3.90 4.06 4.19 4.49 3.89 4.34 4.14 3.70 3-91 4.53 4.47 3.79 161 2.70 104 162 2.57 101 163 2.62 98 164 2.53 102 165 2.61 99 166 2.54 99 167 2.53 103 168 2.55 101 169 2.55 104 170 2.52 105 171 2- .64 98 ~~ i73 2.52 104 174 2.50 102 175 2.51 103 232 NOTOX Project 242933 CLIXICAL BIOCHEkUSTRY. AFTER 4 WEEKS FEMALES GROUP 6 (PFOS) BACK TO MAIN - CHE-IND 12 3 O -MAR- 99 . 177 0.56 2.04 2 i78 0.42 2.09 2 179 0.72 2.36 3 180 0.42 2.48 4 181 0.38 2.45 3 182 0.63 1.85 3 183 0.78 2.32 4 184 0.48 1.52 3 185 0.51 2.59 3 . 186 0.67 2.95 3 187 0.45 2.59 2 189 o .57 2.39 2 19 0 0.61 2.77 2 191 1.67 2.01 3 2.56 0.60 41 1.46 0.30 35 2.29 0.38 38 2.14 0.52 47 1.71 0.58 36 2.48 0.82 41 2.19 0.63 43 1.97 0.63 49 1.96 0.58 44 2.00 0.38 39 1.46 0.33 41 2.47 0.65 42 2.06 0.77 37 1.83 0.39 45 5.31 5.90 4.83 5.02 . 5.85 6.81 4.80 5.38 5.12 5.93 6..3-9 - ~ 7.96 7.71 6.64 11.3 8.6 7.9 12.6 9.1 11.1 9.8 11.6 9.3 9.8 8.2 10.3 8.2 14.4 177 65.0 35.1 29.9 1.2 178 66.6 35.7 30.9 1.2 179 71.5 33.3 38.2 0.9 180 65.3 32.6 32.7 1.0 181 67.5 35.9 31.6 1.1 67.5 34.7 ,32.8 1.1 70.6 37.6 33 .O 1.1 68.8 35.8 33.0 1.1 185 68.8 35.3 33.5 1.1 186 63.9 34.0 29.9 1.1 187 63.1 33.8 29.3 1.2 189 61.2 32.2 29.0 1.1 190 64.5 35.0 29.5 1.2 191 70.3 38.1 32.2 1.2 2.04 3.47 3.85 3.07 3.12 4.80 5.14 4.60 4.12 3.71 7.14 4.32 5.50 4.50 2.55 2.15 2.47 2.69 2.92 2.62 2.64 3.01 2.77 2.74 2.37 2.57 2.36 2.37 143.7 143.4 143.3 142.2 142.9 142.2 142.0 143.4 141.8 144.3 141.9 142.5 141.7 140.0 4.03 3.80 3.77 4.14 4.09 , 4.03 3.82 3.77 4.87 4.05 3.84 4.41 4.27 3.95 177 2.58 178 2.54 179 2.59 180 2.56 181 2.59 182 2.13 183 2.72 . 184 2.62 185 2.63 186 2.49 187 2.57 189 2.59 190 2.53 191 2.63 101 102 99 99 102 103 ' 99 104 99 103 104 102 102 99 233 NOTOX P r o j e c t 2 4 2 9 3 3 CLINICAL BIOCHEKISTRY. A F T W 2 WEEKS RECOVERY GROUP 1 (IKEXICLE CONTROL) BACK TO MAIN - CHE-IND 1 3 3 0-MAR-9 9 10 i.07 2.46 3 11 0.70 3.43 3 2.11 0.46 35 1.39 0.63 28 5.21 7.9 5.95 5.3 ANIMAL NUMBER 9 10 11 ......................................................................................... -T. ALBUMIN GLOBULIN MGRATIO ALP hlow;PHosmIuM POTASSIUM g/l 911 g/l ukat/l rmnol/l Mnol/l ml/l 57.6 28.4 29.2 1.0 4.77 2.87 144.9 4.53 54.9 28.4 26.5 1.1 8.85 2.76 144.9 4.01 55.1 30.1 25.0 1.2 6.16 2.80 143.0 . 4.85 9 2.52 103 10 2.44 102 11 2.48 103 I 234 NOTOX Project 242933 CLINICAL BIOCHEMISrny- AFTER 2 WEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN - CHE-IND 14 30-MAR-99 25 0.71 2.42 3 26 0.58 3.16 3 27 0.56 2.52 2 2.42 0.67 36 1.07 0.39 33 1.53 0.77 32 7.39 6.8 4.33 7.4 6.96 7.7 25 52.0 28.6 23.4 1.2 7.04 2.94 142.1 4.59 26 50.3 27.7 22.6 1.2 7.89 2.76 145.9 4.26 ! 27 52.2 28.0 24.2 1.2 5.86 2.63 142.3 4.69 25 2.50 100 26 2.39 105 27 2.44 102 I .. . 235 NOTOX P r o j e c t 242933 -- BACK TO MAIN - CHE-IND 15 3 0-MAR-9 9 CLJ3IIcAI; BIOCHEMISTRY. AFTER 2 WEEKS RECOVERY .. MAI;ES GRom 3 (TELOMW ACRYLATE) 41 0.61 2.89 3 42 0.55 2.95 3 43 0.78 2.89 2 1.61 1.23 31 1.57 0.89 34 1.54 0.54 33 7.79 6.2 6.81 7.5 5.94 7.1 . . 41 57.5 29.7 27.8 1.1 7.13 2.98 142.6 4.97 42 56.8 29.2 27.6 1.1 43 . 58.6 29.6 29.0 1.0 4.72 5.60 2.73 2.52 142.2 5.07 141.1. 4.82 41 2.51 99 42 2.46 102 43 . 2.47 99 236 NOTOX P r o j e c t 242933 - .- CLINICAL BIOCI-IEMISTRY AFTHi 2 WEEKS RECOVERY MALES GROUP 4 ( 1 - -. . BACK TO MAIN - CHE-IND 16 30 -MAR- 99 . I 57 52.3 27.9 24.4 1.1 6.34 2.73 140.3 4.35 58 57.3 29.1 28.2 1.0. 6.70 2.68 141.2 4.64 59 51.3 26.5 24.8 1.1 4.78 2.52 141.7 . 4.25 57 2.57 99 58 2.56 101 59 2.37 101 237 NOTOX P r o j e c t 242933 C L ~ C A T JB1OCHEMXS"RY~ AFTER 2 WEWS REZOVERY MALES GROUP 5 (PFHS) .. .. BACK TO MAIN - CHE-IND 1 7 3 0 -MAR-9 9 73 53.9 20.4 25.5 1.1 5.85 2.67 142.1 4.71 74 55.1 28.2 26.9 1.0 6.09 2.43 143.4 4.57 75 55.1 30.0 25.1 1.2 7.43 2.42 . 141.7 . 4.41 73 2.44 101 74 . -2.49 104 75 2.42 100 238 NOTOX Project 242933 CLINICAL BIOCBEMIS'JXY' AFTER 2 WEEKS RECOVERY MAz;ES GROUP 6 (PPOS) BACK TO MAIN - CHE-IND 1 8 30-MAR-99 .. 89 0.83 90 0.61 91 . 0.49 2.48 2 1.63 3 1.66 3 1.22 0.51 34 1.48 0.40 34 1.12 0.60 49 7.21 7.3 6.29 6.7 5.17 8.1 a9 56.3 30.0 26.3 1.1 90 55.9 29.0 26.9 1.1 91 51.3 30.1 21.2 1.1 6.36 2,52. 142.8 4.52 4.22 2.67 142.7 4.82 6.26 2.75 142.0 . 4.35 89 2.53 101 90 2.65 102 91 2.53 JO5 239 NOTOX P r o j e c t 242933 - - .. -.-.,a CLINICAL BIOCHEMISTRYAFTW 2 WEEKS RECOVERY FEMlLES GROUP 1 (vEErcI;E CONTROL) BACK TO MAIN - CHE-IND 1 9 3 0 -MAR- 99 . 105 0.52 2.50 3 106 0.43 1.96 3 107 0.46 3.12 2 1.95 0.56 37 2.59 0.97 38 1.93 0.55 41 6.72 6.7 9.25 6.2 6.06 7.6 105 65.2 32.4 32.8 1.0 3.43 2.45 143.3 3.98 106 65.3 ' 34.7 30.6 1.1 3.21 2.39 142.5 4.14 107 .60.4 29.4 31.0 0.9 . 3.26 2.56 141.4 . 4.12 105 2.60 98 106 2.79 99 107 2.51 99 .. ; 240 NOTOX Project 242933 CLINICAL BIOCHEMISTRY. AFTER 2 WEEKS RECOVERY FEM24LES GROUP 2 (TELOMER ALCOHOL) - - .. n BACK TO MAIN -- CHE-IND 20 30-MAR 9 3 121 0.52 2.44 2 122 1.02 3.36 3 123 . 0.36 2.02 2 2 .a4 0.43 40 2.20 0.40 41 1.42 0.46 38 7.26 6.3 6.31 8.9 5.98 6.7 1_ _2_1 58.1 29.6 28.5 1.0 122 61.3 34.9 26.4 1.3 123 65.6 33.6 32.0 1.1 4.38 2.64 4.07 2.89 2.34 2.18 142.4. 3.95 144.2 4.16 141.7 3.77 121 2.54 98 122 2.65 100 123 2.59 99 241 NOTOX Project 242933 - .- 4 CLINICAL BIOCHEMISTRY . AFTER 2 wEEs RECOVERY FEMALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN - CHE-IND 21 - 3 0-MAR 99 137 0.58 2.27 2 13 a 0.51 2.35 2 139 0.37 1.69 2 1.27 0.39 37 1.97 0.87 40 1.97 0.57 47 6.71 7.1 7.44 9.3 7.77 8.1 137 60.7 29.5 31.2 0.9 13 a 62.9 32.5 30.4 1.1 13 9 59.7 32.9 26.8 1.2 5.56 2.27 4.02 . 2.38 3.49 2.30 143.1 3.76 141.4 4.54 141.5 3.86 137 2.46 9a 13 8 2.61 103 139 2.63 96 242 NOTOX P r o j e c t 242933 - ....- CLJXICAL BIOCHEMISTRY - A F m 2 WEEKS RJEOVERY FEMALES GROUP 4 ( 1 BACK TO MAIN - CHE-IND 2 2 3 0-MAR-99 .. 153 0.48 2.35 3 154 0.63 2.32 2 155 0.60 2.93 3 2 :17 1.03 43 2.72 0.53 39 2.34 0.63 53 7.47 0.41 6.34 9.5 8.9 11.0 I 153 67.8 35.7 32.1 1.1 3.09 2.70 142.7 3.90 154 61.8 32.4 29.4 1.1 4.81 2.23 139.7 4.61 155 66.1 33.4 32.7 1.0 3.65 2.71 141.4 4.50 153 2.69 100 154 2.64 98 155 2.70 97 243 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 5 (PFHS) BACK TO MAIN - CHE-IND 23 3 0 -MAR- 99 169 0.48 1.83 3 170 0.43 1.56 3 171 0.65 1.58 . 3 1;66 0.50 46 2.42 0.35 39 2.24 0.34 47 7.25 7.7 6.01 7.2 9.38 7.9 ANIMAL NUMBER 169 170 171 CALCIUM mm1/1 CHLORIDE mpol/l 2.58 100 2.62 99 2.84 95 244 NOTOX P r o j e c t 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) BACK TO MAIN .. - CHE-IND 2 4 30-MAR-99 185 0.37 2.22 2 ' 2.04 0.40 I43 186 0.51 2.15 3 2.37 0.25 41 187 0.48 1.89 2 1.64 0.29 42 8.03 8.2 7.67 9.0 7.37 6.7 ANIMAL NUMBER 185 186 187 ......................................................................................... PROTEINT. ALE= g/l d 1 GLOBULIN N G R A T I O ALP g/l ukat/l INDRGPHOSSODIUM m l / l mnol/l POTASSIUM ml/l 66.6 33.9 32.7 1.0 57.4 31.6 25.8 1.2 57.9 32.0 25.9 1.2 3.66 2.40 141.9 3.97 3.25 2.21 ,143.4 4.27 6.04 2.27 142.2 4.08 185 2.65 99 186 2.56 103 187 2.69 99 245 NOTOX Project 242933 CLINICAL BIO<SBEMISTRY . AFTER 4 WEEKS RECOVERY MALIES GROUP 1 (VEEIICIZ CONTROL) BACK TO MAIN - CHE-IND 25 - 30-MdB 9 9 . 13 0.78 2.88 4 14 0.54 2.39 3 15 0.44 2.69 3 2.01 1.17 37 8-42 6.1 2.01 0.39 35 . 7.63 6.1 1.73 0.71 37 7.97 6.7 13 62.1 29.3 32.8 0.9 14 62.2 28.2 34.0 0.8 15 62.2 28.4 33 .a 0.8 4.61 2.91 4.79 3.17 3.58 2.99 140.8 5.15 142.5 5.56 141.6 5.28 13 2.56 104 14 2.60 . 101 15 2.58 102 246 NOTOI Project 242933 CLmCAL BIOCHEMISTRY. AFTER 4 WEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN .. - CHE-IND 26 3 0-MAR-9 9 29 0.70 2.66 2 30 0.61 2.49 2 31 0.74 2.17 ' 3 1.39 0.57 29 1.22 0.55 33 1.14 0.61 36 5.87 6.5 8.32 6.3 8.16 6.2 29 57 .a 26.7 31.1 0.9 30 54.2 27.5 26.7 1.0 31 55.6 27.7 27.9 1.0 6.15 2.44 143.4 4.53 8.13 2.51 141.4 4.50 4.31 2.96 143.9 4.75 29 2.44 106 30 2.46 103 31 2.55 101 .. 247 NOTOX Project 242933 BACK TO MAIN - CHE-IND 27 30-MAR- 99 45 0.62 2.00 3 46 0.57 2.33 2 47 0.60 1.86 3 1.93 0.42 32 1.16 0.53 39 1.51 0.64 31 7.91 7.4 7.38 6.7 5.08 5.6 45 58.4 27.7 30.7 0.9 46 51.6 25.4 26.2 1.0 47 56.2 27.9 2a .3 1.0 3.03 2.67 141.4 .. 4.06 6.75 2.86 143 .a 4.81 5.27 2.38 142.3 4.26 45 2.53 102 46 2.47 105 47 2.44 104 I J 2 4 8. NOTOX P r o j e c t 242933 CLINICAL BIOCHEMISTRY. AFTER 4 lmEKs RECOVERY MALES GRom 4 ( 1 BACK TO MAIN - CHE-IND 28 30-MAR- 99 .. 61 0.67 2.96 3 62 0.38 2.16 2 63 0.69 2.05 . 3 1.37 0.55 39 2.05 0.59 37 1.56 0.55 39 5.58 6.9 6.68 0.2 6.11 7.3 61 59.5 29 .O 30.5 1.0 62 54.9 27.7 27.2 1.0 63 56.6 26.5 30.1 0.9 4.68 2.46 . 141.5 4.61 4.66 2.64 141.8 4.62 6.94 2.28 142.7 4.41 ANIMAL NUMB? 61 62 63 CACCIUM CHLORIDE m l / l mnol/l 2.49 103 2.45 . 104 2.44 105 249 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES G R O U P S (PFHS) BACK TO MAIN - CHE-IND 29 30-MAR- 99 77 0.60 2.61 3 78 0.94 2.21 2 79 0.71 2.38 2 1.22 0.57 47 1.34 0.26 47 1.16 0.24 36 5.95 8.1 4.77 10.4 6.00 6.5 77 57.9 27.0 30.9 0.9 78 58.1 28.0 30.1 0.9 79 56.0 27.0 29.0 0.9 5.79 2.55 143.5 4.40 4.17 2.55 142.9 4.26 8.04 2.86 144.9 5.04 77 2.48 104 78 2.49 103 79 2.51 103 250 NOTOX P r o j e c t 242933 C L m C A L BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 6 (PPOS) - - . .. BACK TO MAIN - CHE-IND 3 0 3 0 -MAR- 9 9 93 0.56 1.99 2 94 0.77 . 1.73 2 95 0.58 1.89 2 0.92 0.35 29 1.47 0.58 31 1.62 0.43 36 6.01 5.8 7.23 5.6 6.85 6.5 ANIMAL NUMBER 93 94 9s ......................................................................................... PRI)TEINT.ALBUMTN GLOBULIN I v G R A T I O ALP INORG PIDS SODIUM PO'IASSIUM g/l g/l g/l ukat/l m 1 / 1 m 1 / 1 m W l / l 51.7 27.0 24.7 1.1 55.0 27.3 27.7 1.0 56.7 28.3 28.4 1.0 4.89 2.58 142.8 4.60 4.78 2.40 142.7 4.78 4.52 2.79 ' 142.5 4.58 93 . 2.43 105 94 2.52 107 95 2.49 109 251 CLIMCAL BIOCHEMISTRY AFTER 4 WEWS RECOVERY FEMALES GROUP 1 (llEEIcLE CONTROL) - - .. BACK TO MAIN - CHE-IND 31 30-MAR- 99 109 61.4 30.1 31.3 1.0 110 69.4 33.2 36.2 0.9 111 63.8 ' 30.5 33.3 0.9 3.41 2.46 144.0 3.84 2.49 2.14 142.5 4.01 2.96 2.39 140.5 4.50 109 2.47 103 110 2.55 101 111 ,2.55 96 252 NOTOX P r o j e c t 242933 CLINICAL BIOCHEMISTRY. AFTER 4 WEEXs ~ C O V E R Y -s GFt0I.JP 2 ("ELOMER ALCOHOL) BACK TO MAIN - CHE-IND 32 3 0 -MAR-9 9 125 0.39 2.66 2 126 127 0-.-3-4 2.26 2 2.01 . 3 2.68 1.00 39 2.01 0.47 40 2.55 . 0.76 4a 6.83 6.7 6.54 6.8 5.91 9.0 125 67.0 32.2 34.8 0.9 126 62-2 30.3 31.9 0.9 127 63.6 32.4 31.2 1.0 1.73 2.26 141.4 4.25 3.18 2.35 142.2 4.36 4.19 2.11 141.3 3.82 125 2.64 104 12 6 2.49 98 127 2.46 102 253 NOTOX Project 242933 CLINICAL BIOCHeMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 3 ("ELOMEFt ACRYLATE) BACK TO MAIN - CHE-IND 33 - 30-MAP 0 9 141 0.43 1.74 2 142 0.36 1.54 3 143 0.59 2.40 3 2.18 0.43 40 2.39 0.50 41 2.32 - 0.49 45 6.64 a -3 6.73 6.9 7.32 , 8.8 141 . 65.5 29.5 36.0 0.8 142 67.8 34.7 33.1 1.0 143 69.9 35.9 34.0 1.1 3 .a6 2.08 3.40 2.19 138.7 . 5.43 1.95 141.6 3.66 1.79 ' 141.7 4.34 141 2.47 96 142 2.60 103 143 2.59 102 .. 254 NOTOX Project 242933 -..-\- CLINICAL BIOCHEMISTRY. AFTER4wEEKsRECOvERY ' FEMALlES GROUP 4 ( 1 BACK TO MAIN - C H E - I N D 3 4 - 3 0-MAR 99 157 0.43 2.75 2 158 0.98 2.30 2 159 0.47 2.31 2 2.99 0.41 43 2.52 0.40 40 2.02 0.72 48 5.89 8.2 8.08 6.9 5.05 9.9 157 64.8 30.3 34.5 0.9 158 68.9 32.0 36.9 0.9 159 62.2 29.4 ' 32.8 p.9 2.48 2.20 141.0 4.51 3.91 1.93 142.1 3.67 2.40 2.52 140.9 4.59 157 2.60 100 158 2.57 96 159 2.53 101 255 NOTOX P r o j e c t 242933 CLINICAL BIOCHEMISTRY. AETER 4 WEWS RECOVERY FEMALES G R O U P 5 (PFHS) , BACK TO MAIN - CHE-IND 3 5 30-MAR- 99 173 0.33 2.27 2 174 0.64 3.09 2 175 0.45 2.21 2 1.68 0.40 38 2.12 0.34 39 2.37 0.46 39 - 6.77 6.8 6.50 6.9 7.31 7.3 173 68.5 34.9 33.6 1.0 174 69.4 33.5 35.9 0.9 175 70.2 34.2 36.0 1.0 1.78 2.15 139.9 4.17 3.46 2.08 140.5 4.25 2.05 2.24 142.6 3.65 173 2.59 104 174 2.59 99 175 2.61 102 I 256 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTW 4 WEEKS RECOVERY FEMALES GROUP 6 (PPOS) - - .. BACK TO MAIN - CHE-IND 36 30-MAR-93 189 0.52 1.96 2 190 0.49 1.94 2 191 0.50 1.86 3 2.38 0.39 39 1.99 0.74 38 2.01 0.37 39 6.77 9.4' 8.87 7.9 7.31 14.9 189 . 59.5 30.6 28.9 1.1 190 66.2 33.2 33.0 1.0 191 70.1 34.6 35.5 1.0 3.56 2.56 142-.7 4.13 3.19 2.18 141.0 4.36 3.40 2.07 139.5 4.00 189 2.63 100 190 2.61 100 191. 2.67 100 2 5 7. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ - c-/PFHS/PFO< MACROSCOPIC FINDINGS MALES GROUP 1 (VEHICLE CONTROL) ANIMAL 1 BACK TO MAIN - MAC-IND 1 25 -FEB- 99' (SCHEDULED NECROPSY, 03-NOV-98) NO FINDINGS NOTED ANIMAL 2 (SCHEDULED NECROPSY, 03-NOV-98) NO FINDINGS NOTED ANIMAL 3 (SCHEDULED NECROPSY, 03-NOV-98) NO FINDINGS NOTED ANIMAL 4 I NO FINDINGS NOTED (SCHEDULED NECROPSY, 03-NDV-98 ) ANIMAL 5 NO FINDINGS NOTE0 (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 6 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 7 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 8 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 9 SKIN ................ CHEST REGION: ALOPECIA. ANIMAL 10 (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-N0b-98) NO FINDINGS NOTED ANIMAL 11 (SCHEDULED NECROPSY, 18-NOV-98) NO FINDINGS NOTED 258 - NOTOX Project 242933 - TELOMER ALCOHOLITELOMER ACRYLATE/ - .- /PFHS/PFOS MACROSCOPIC FINDINGS MALES GROUP 1 (VEHICLE CONTROL) ANIMAL 13 NO FINDINGS NOTED ANIMAL 14 NO FINDINGS NOTED ANIMAL 15 NO FINDINGS NOTED BACK TO MAIN .. MAC-IND - 2, 25-FEE- 99 .. (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 259 NOTOX P r o j e c t 242933 - .. TELOMER ALCOHOL/TELOMER ACRYLATE/ - c-./PFHS/PFOS BACK TO MAIN - MAC-IND 3. 25- FEB-99 MACROSCOPIC FINDINGS MALES GROUP 2 (TELOMERALCOHOL) ANIMAL 17 (SCHEDULED NECROPSY, 03-NOV-98) NO FINDINGS NOTED ANIMAL- 18 (SCHEDULED NECROPSY, 03-NOV-98) NO FINDINGS NOTED ANIMAL 19 NO FINDINGS NOTED . ANIMAL 20 NO FINDINGS NOTED (SCHEDULED NECROPSY, 03-NOV-98) - (SCHEDULED NECROPSY, 03-NOV-98) ANIMAL 2 1 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 2 2 NO FINDINGS NOTED ANIMAL 23 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) ~~ ~~ ~~ ~ ANIMAL 2 4 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 25 NO FINDINGS NOTED ANIMAL 26 NO FINDINGS NOTED ANIMAL 2 7 TESTES. ............. REDUCED I N SIZE, FLACCID. (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) ~ (SCHEDULED NECROPSY, 18-NOV-98) 260 NOTOX Project 242933 d TELOMER ALCOHOLITELOMER ACRYLATE/ - --IP-FHSIPFOS MACROSCOPIC FINDINGS MALES GROUP 2 (TELOMER ALCOHOL) ANIMAL 29 NO FINDINGS NOTED ANIMAL 30 NO FINDINGS NOTED ANIMAL 31 NO FINDINGS NOTED BACK TO MAIN -. MA2C5-I- FNEDB-- 949, (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) . 261 NOTOX P r o j e c t 242933 - TELOMER ALCOHOL /TELOMER ACRYLATE/ - ./-PFHS/PFOS MACROSCOPIC FiNDlNGS MALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 33 NO FINDINGS NOTED ANIMAL 34' NO FINDINGS NOTED ANIMAL 35 NO FINDINGS NOTED ANIMAL 36 NO FINDINGS NOTED ANIMAL 37 NO FINDINGS NOTED ANIMAL 38 . NO FINDINGS NOTED ANIMAL 39 NO FINDINGS NOTED ANIMAL 40 ' NO FINDINGS NOTED ANIMAL 4 1 NO FINDINGS NOTED ANIbiAL 42 NO FINDINGS NOTED ANIMAL 43 NO FINDINGS NOTED BACK TO MAIN - . . MAC-IND 5 25-FEB-99 (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) 262 - - NOTOX P r o j e c t 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ - IPFHS~PFOS BACK TO MAIN MAC-IND - 6 25 -FEE -99 MACROSCOPIC FINDINGS MALES GROUP 3 (TELOMER ACRYLATE) -ANIMAL 45 (SCHEDULED NECROPSY, 02-DEC-98) NO FINDINGS NOTED ANIMAL 46 (SCHEDULED NECROPSY, 02-DEC-98) NO FINDINGS NOTED ANIMAL 47 (SCHEDULED NECROPSY, 02-DEC-98) NO FINDINGS NOTED ! , . .. 263 NOTOX Project 242933 TELOMER ALCOHOLlTELOMER ACRYLATE. - I IPFHSIPFOS BACK TO MAIN MAC-IND - 7. 25-FEB- 9 9 MACROSCOPIC FINDINGS MALES GROUP4( ,) L . ANIMAL49 ~ NO FINDINGS NOTED ANIMAL50 . ~ ~~ NO FINDINGS NOTED ANIMAL 5 1 NO F I N D I W NOTED ANIMAL 52 NO FINDINGS NOTED ANIMAL 53 NO FINDINGS NOTED ANIMAL 54 NO FINDINGS NOTED ANIMAL 55 NO FINDINGS NOTED ANIMAL 56 ' NO FINDINGS NOTED ANIMAL 57 NO FINDINGS NOTED ANIMAL 58 NO FINDINGS NOTED ANIMAL 59 NO FINDINGS NOTED (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSYl 03-NOV-98) ~~~ (SCHEDULED NECROPSY, 03-NOV-90) ~ ~~ ~ (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSYI 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) 264 - NOTOX Project 242933 TELOMER ALCOHOL /TELOMER ACRYLATE/ - .- /PFHS/PFOS MACROSCOPIC FINDINGS MALES GROUP4( ) ANIMAL 61 ~ EYES................ RIGHT SIDE, LENS: REDUCED IN SIZE. ANIMAL 62 -~ NO FINDINGS NOTED ANIMAL 63 NO FINDINGS NOTED BACK TO MAIN .. MAC-IND - 8 25 -FEB-99 (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 265 NOTOX P r o j e c t 242933 TELOMER ALCOHOUTELOMER ACRYLATE/ - IPFHSIPFOS -I - BACK TO MAIN MAC-IND 9 25-FEB -99' MACROSCOPIC FINDINGS MALES GROUP 5 (PFHS) ANIMAL 65 (SCHEDULED NECROPSY, 03.NOV -98 ) NO FINDINGS NOTED ANIMAL 66 LUNGS ............... STOMACH.. ........... TESTES .............. EPIDIDYMIDES ........ HEMORRHAGE. GLANDULAR MUCOSA: HEMORRHAGE. REDUCED I N SIZE. REDUCED I N SIZE. 1 ANIMAL 6 7 NO fINDINGS NOTED (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) ANIMAL 68 (SCHEDULED NECROPSY, 03-NOV-98) NO FINDINGS NOTED ANIMAL 69 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 70 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 71 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 72 LIVER ............... ENLARGED. (SCHEDULED NECROPSY, 04-NOV-98) ANIMAL 73 NO FINDINGS NOTED (SCHEDULED NECROPSY, 18-NOV-98) ANIMAL 74 TESTES .............. REDUCED IN SIZE. (SCHEDULED NECROPSY, 18-NOV-98) 266 NOTOX Project 242933 .. TELOMER ALCOHOLITELOMER ACRYLATE/- ~PFHS~PFOS c- BACK TO MAIN MAC-IND -10 25-FEB-99' MACROSCOPIC FINDINGS MALES GROUP 5 (PFHS) ANIMAL 75 -~ NO FINDINGS NOTED ANIMAL n (SCHEDULED NECROPSY, 18-NOV-98) ~ (SCHEDULED NECROPSY, 02-DEC-98) NO FINDINGS NOTED ANIMAL 78 (SCHEDULED NECROPSY 02-DEC-98) NO FINDINGS NOTED . ANIMAL 79 (SCHEDULED NECROPSY 02-DEC-98) NO FINDINGS NOTED 267 - - NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHSIPFOS L MACROSCOPIC FINDINGS MALES GROUP 6 (PFOS) ANIMAL 8 1 NO FINDINGS NOTED ANIMAL 82 NO FINDINGS NOTED ANIMAL 83 NO FINDINGS NOTED ANIMAL 84 NO FINDINGS NOTED ANIMAL 85 NO FINDINGS NOTED ' ANIMAL 86 NO FINDINGS NOTED ANIMAL 87 NO FINDINGS NOTED ANIMAL 88 ' NO FINDINGS NOTED ANIMAL 89 NO FINDINGS NOTED ANIMAL 90 LIVER;. ............. ANIMAL 9 1 ACCENTUATED LOBULAR PATTERN. NO FINDINGS NOTED BACK TO MAIN MAC-IND -11. 25 -FEB-99 (SCHEDULED NECROPSY, - 03-NOV 98 ) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) ~ ~~~ (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) (SCHEDULED NECROPSY, 18-NOV-98) NOTOX P r o j e c t 242933 - TELOMER ALCOHOLlTELOMER ACRYLATE/- . I IPFHSIPFOS MACROSCOPIC FINDINGS MALES GROUP 6 (PFOS) ANXMAL 93 NO FINDINGS NOTED ANIMAL 94 NO FINDINGS NOTED ' ANIMAL 95 NO FINDINGS NOTED BACK TO MAIN .. MAC-IND -12. 25-FEB-99 (SCHEDULED NECROPSY, 02-OEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 269 !. NOTOX P r o j e c t 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ .. IPFHWPFOS BACK TO MAIN MAC-IND -13 25 - FEB -99 MACROSCOPIC FINDINGS FEMALES . GROUP 1 (VEHICLE CONTROL) ANIMAL 97 NO FINDINGS NOTED ANIMAL 98 OVARIES ............. LEFT SIDE: WATERY CYST. ANIMAL 99 NO FINDINGS NOTED ANIMAL 100 NO FINDINGS NOTED ANIMAL 1 0 1 NO FINDINGS NOTED ANIMAL 102 ~~ NO FINDINGS NOTED ANIMAL 103 NO FINDINGS NOTED ANIMAL 104 NO FINDINGS NOTED ANIMAL 105 SKIN ................ ANIMAL 106 ABDOMINAL REGOIN: SCAB FORMATION. NO FINDINGS NOTED ANIMAL 107 NO FINDINGS NOTED (SCHEDULED NECROPSY, 05-NOV-98 ) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY. 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) ~~~~ ~ (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) 270 NOTOX P r o j e c t 242933 JPFHSIPFOS TELOMER ALCOHOL~TELOMERACRYLATE/ -Y MACROSCOPIC FINDINGS FEMALES . GROUP 1 (VEHICLE CONTROL) ANXMAL 109 BACK TO MAIN .. - MAC-IND -14 25 - FEB 99 (SCHEDULED NECROPSY, 04-DEC-98) NO FINDINGS NOTED ANIMAL 110 (SCHEDULED NECROPSY, 04-DEC-98) NO FINDINGS NOTED ANIMAL 111 (SCHEDULED NECROPSY, 04-DEC-98) NO FINDINGS NOTED 271 NOTOX P r o j e c t 242933 TELOMER ALCOHOL lTELOMER ACRYLATE1 - --IP-FHSIPFOS- MACROSCOPIC FINDINGS FEMALES GROUP 2 (TELOMER ALCOHOL) ANIMAL 113 NO FINDINGS NOTED ANIMAL 114 NO FINDINGS NOTED ANIMAL. 115 NO FINDINGS NOTED ANIMAL 116 NO FINDINGS NOTED ANIMAL 117 KIDNEYS............. RIGHT SIDE: PELVIC DILATION. ' ANIMAL 118 NO FINDINGS NOTED ANIMAL 119 NO FINDINGS NOTED ANIMAL 120 ' NO FINDINGS NOTED ANIMAL 121 NO FINDINGS NOTED ANIMAL 122 SKIN.. .............. ALOPECIA. ANIMAL 123 NO FINDINGS NOTED BACK TO MAIN .. MAC-IND -15 25-FEB-99' (SCHEDULED NECROPSY, 05-NOV-98) - (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY; 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEWLED NECROPSY, 06-NOV-98) (SCHEWLED NECROPSY, 20-NOV-98) . (SCHEDULED NECROPSY, 20-NOV-98) (SCHEWLED NECROPSY, 20-NOV-98) 272 I NOTOX Project 242933 .. TELOMER ALCOHOLITELOMER ACRYLATE/ IPFHSIPFOS- -C I BACK TO MAIN - MAC-IND -16 25-FEB 99 MACROSCOPIC FINDINGS FEMALES GROUP 2 (TELOMER ALCOHOL) ANIMAL 125 ~~ NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-DEC-98) ANIMAL 126 ~~ NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-DEC-98) ANIMAL 127 ~ NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-OEC-98) 273 NOTOX Project 242933 TELOMER ALCOHOLI TELOMER ACRYLATE/ PFHS~ P F O S BACK TO MAIN - - MAC-IND -17 25 FEB 99' MACROSCOPIC flNDlNGS FEMALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 129 (OTHER, 05-NOV-98) NO FINDINGS NOTED ANIMAL 130 NO FINDINGS NOTED .ANIMAL 131 (SCHEDULED NECROPSY, 05-NOV-98) .. (SCHEDULED NECROPSY, 05-NOV-98) NO FINDINGS NOTED ! ANIMAL 132 NO FINDINGS NOTED ANIMAL 133 NO FINDINGS NOTED ANIMAL 134 ~~~~~ ~ NO FINDINGS NOTED ANIMAL 135 NO FINDINGS NOTED 3 ANIMAL 136 t No FINDINGS NOTED ANIMAL 137 NO FINDINGS NOTED ANIMAL 138 NO FINDINGS NOTED ANIMAL 139 NO FINDINGS NOTED (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) ~ (SCHEDULED NECROPSY, 20-NOV-98) ~~ - (SCHEDULED NECROPSY, 20-NOV-98) 274 NOTOX P r o j e c t 242933 TELOMER ALCOHOL~TELOMERACRYLATE/ MACROSCOPIC FINDINGS FEMALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 141 NO FINDINGS NOTED ANIMAL 142 NO FINDINGS NOTED ANIMAL 143 NO FINDINGS NOTED .. IPFHS~PFOS- BACK TO MAIN - MAC-IND -18 25 FEB -99 (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) I .. 275 NOTOX Project 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ :PFHS/PFO< MACROSCOPIC FINDINGS FEMALES GROUPQ( .) ANIMAL 145 NO FINOINGS NOTED ANIMAL 146 NO FINDINGS NOTED ANIMAL 147 NO FINDINGS NOTED ANIMAL 148 NO FINDINGS NOTED ANIMAL 149 NO FINDINGS NOTED ANIMAL 150 NO FINDINGS NOTED ANIMAL 1 5 1 UTERUS.. ...,........ LEFT SIDE: CYST(S). ANIMAL 1 5 2 NO FINDINGS NOTED ANIMAL 153 BACK TO MAIN MAC-IND -19 25-FEB- 99 (SCHEDULED NECROPSY, 05-NOV-98 ) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05 -NDV- 9 8 ) (SCHEDULED NECROPSY, 06-NOV-98) . (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) ANIMAL 1 5 4 NO FINDINGS NOTED ANIMAL 155 ~ NO FINDINGS NOTED (SCHEDULED NECROPSY, 20 -NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) ~ 276 ~~ NOTOX P r o j e c t 242933 .. TELOMER ALCOHOLITELOMER ACRYLATE1 IPFHSIPFOS- - -\.- -- BACK TO MAIN MAC-IND -20 25-FEB- 99 MACROSCOPIC FINDINGS FEMALES GROUP4( ) ANIMAL 157 (SCHEDULED NECROPSY, 04-DEC-98) NO FINDINGS NOTED ANIMAL 158 (SCHEDULED NECROPSY, 04-DEC-98 ) NO FINDINGS NOTED ANIMAL 159 (SCHEDULED NECROPSY, 04-OEC-98) NO FINDINGS NOTED 277 NOTOX Project 242933 TELOMER ALCOHOLITELOMER ACRYLATE/ IPFHS~PFOS BACK TO MAIN - MAC-IND -21 25 FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 5 (PFHS) ANIMAL 161 NO FINDINGS NOTED ANIMAL 162 NO .FINDINGS NOTED ANIMAL 163 NO FINDINGS NOTED ANIMAL 164 NO FINDINGS NOTED ANIMAL 165 NO FINDINGS NOTED ANIMAL 166 NO FINDINGS NOTED ANIMAL 167 NO FINDINGS NOTED ANIMAL 168 NO FINDINGS NOTED ANIMAL 169 NO FINDINGS NOTED ANIMAL 170 NO FINDINGS NOTED ANIMAL 171 NO FINDINGS NOTED .. (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) .. (SCHEDULED NECROPSY, 06-NOV-98) >- (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) 270 NOTOX P r o j e c t 242933 - TELOMER ALCOHOLITELOMER ACRYLATE/ - L IPFHSIPFOS MACROSCOPIC FINDINGS FEMALES GROUP 5 (PFHS) ANIMAL 173 NO FINDINGS NOTED ANIMAL 174 . . NO FINDINGS NOTED ANIMAL 175 NO FINDINGS NOTED BACK TO MAIN MAC-IND -22, 25-FEB- 99 (SCHEDULED NECROPSY, 04-DEC-98 ) (SCHEDULED NECROPSY, -0 4 DEC- 9 8 ) (SCHEDULED NECROPSY, 04-DEC-98) 279 NOTOX P r o j e c t 242933 -- TELOMER ALCOHOL/TELOMER ACRYLATE/ - -.- rPFHS/PFOS MACROSCOPIC FINDINGS FEMALES GROUP 6 (PFOS) ANIMAL 177 NO FINDINGS NOTED ANIMAL 178 BACK TO MAIN - MAC-IN0 -23 25 - FEB 99 (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-NOV-98) ANIMAL 179 (SCHEDULED NECROPSY, 05-NOV-98) ANIMAL 180 NO FINDINOS NOTED ANIMAL 181 NO FINDINGS NOTED ANIMAL 182 NO FINDINGS NOTED ANIMAL ia3 NO FINDINGS NOTED ANIMAL 184 ... NO FINDINGS NOTED ANIMAL 185 NO FINDINGS NOTED ANIMAL 186 (SCHEDULED NECROPSY, 05-NUV-98) (SCHEDULED NECROPSY, 06-"4-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) (SCHEDULED NECROPSY, 20.NOV-98) ANIMAL 187 NO FINDINGS NOTED (SCHEDULED NECROPSY, 20-NOV-98) . 280 NOTOX Project 242933 - TELOMER ALCOHOL ITELOMER .. - ACRYLATE/ IPFHSIPFOS I .- MACROSCOPIC FINDINGS FEMALES GROUP 6 (PFOS) ANIMAL 189 NO FINDINGS NOTED ANIMAL 190 NO FINDINGS NOTED ANIMAL 191 NO FINDINGS NOTED BACK TO MAIN .. MAC-IND -24 25 -FEB-99 (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) 281 NOTOX Project 242933 BACK TO MAIN - OW-IND 1 30-MAR-9 9 GROUP 1 (VEEICLE CONTROL) ANIMAL NoMBER BODY W. BRAIN HEART 1 284 2 332 3 348 4 270 5 3 03 '6 327 7 351 8 301 1.95 1.081 2.04 1.390 2.03 1.108 2.00 1.070 1.92 1.141 2.01 1.088 2.14 1.123 1.99 1.059 GROUP 2 (TELOMHZ ALCOHOL) ANIMAL NUMBER BODYW. BRAIN HEART 17 ' 322 1.99 i- _a 2-9_5- 2.01 19 312 2.00 20 299 1.96 21 315 1.84 22 348 2.05 23 3 13 1.87 24 312 . 1.95 1.149 1.300 0.935 1.033 1.129 1.309 0.972 1.241 LIVER 8.04 10.76 10.40 8.54 10.49 10.38 11.92 9.84 LIVER 10.16 11.71 9.71 9.50 10.48 10.71 10.15 10.42 KIDNEYS ADRENAts SPLEEN TESTES 1.84 0.038 0.434 2.71 2.59 0.050 0.565 2.79 2.41 0.037 0.563 3.32 1.75 0.040 0.482 2.53 2.54 0.053 0.488 2.91 2.06 0.044 0.733 2.82 2.63 0.051 0.766 3.03 2.59 0.047 0.605 2.82 KIms AaRFNAts SPLEEN TESTES 2.35 0.049 0.465 3.05 2.57 0.050 0.551 3.27 2.35 0.053 0.541 3.19 2.24 0.055 0.540 3.21 2.40 0.050 0.565 2.63 2.57 0.049 0.712 3.18 2.12 0.072 0.560 2.87 2.09 0.049 0.590 2.96 33 3 13 34 288 35 293 36 293 , ,: 37 38 303 324 39 319 40 304 GFtOUP 1.97 1.93 1.95 1.94 2.02 1.98 I 1.99 1.82 1.127 9.89 2.08 0.943 8.88 2.16 1.131 8.71 2.08 0.972 8.90 2.14 1.321 9.66 2.50 1.079 10.40 2.37 1.077 9.48 2.19 1.122 8.95 2.12 0.053 0.046 0.038 0.059 0.098 0.056 0.046 0.056 0.637 0.498 0.476 0.530 0.603 0.592 0.656 0.547 2.52 2.77 2.77 2 -74 2.84 2.97 49 50 5_ -1 52 53 54 55 . 56 290 1.96 296 2.07 3- 4_2_ 2.11 324 ' 1.97 320 1.98 330 1.99 284 1.97 2 63 1.93 1.055 8.23 1.99 1.193 9.16 2.08 1.156 10.83 2.54 1.224 9.52 2.26 1.331 9.39 2.36 1.207 9.53 2.25 1.025 8.50 2.07 1.043 7.18 1.73 0.046 0.488 3.18 0.033 0.627 3.00 0.046 0.719 2.77 0.053 0.559 2.96 0.042 0.629 2.58 0.041 0.547 2.70 0.045 0.537 2.95 0.058 0.519 2.75 282 NOTOX Project 242933 ORGANWEIGHTS (GRAM) . AFTER4wEEKs MALES GROUP 5 (PFHS) BACK TO MAIN - OW-IND 2 30-MAR-9 9 65 352 2.02 66 270 1.89 67 297 2.03 68 297 1.94 69 287 1.86 70 . 347 2.07 71 311 1.83 72 306 2.04 GROUP 6 (PFOS) 1.267 16.85 2.77 0.037 0.595 3.22 0.992 11.52 2.14 0.038 0.504 0.85 1.131 13.86 2.49 0.059 0.549 3.18 1.148 13.10 2.31 0.046 0.541 3.07 1.096 12.96 2.11 . 0.058 0.570 2.82 1.153 14.49 2.47 0.057 0 -745 3.18 1.030 11.84 2.19 0.051 0.516 3.05 1.040 15.62 2.28 0.056 0.539 3.04 81 303 82 295 83 3 08 84 318 85 3 62 86 293 87 351 88 317 1.99 1.102 11.04 2.15 0.039 0,501 2.80 1.98 1.165 11.17 2.21 0.055 0.433 2.86 2.06 0.961 10.67 1.91 0.039 0.560 2.82 2.00 1.171 11.52 2.42 0.032 0.510 2.79 1.96 1.223 13.46 2.34 0.060 0.692 2.88 1.93 1.259 12.90 2.28 0.039 0.664 2.58 1.97 1.163 12.33 2.26 0.067 0.634 3.22 2.07 1.004 11.19 1.96 0.040 0.469 2.84 283 NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS MALES GRom 1 (VEHICLE CONTROL) BACK TO MAIN O W 3 0 IND -MAR - - 9 3 9 .. 1 284 0.69 0.381 2 332 0.61 0.419 3 348 0.58 0.318 4 270 0.74 0.396 5 303 0.63 0.377 6 327 0.62 0.333 '7 351 0.61 0.320 8 301 0.66 0.352 GROUP 2 (TELOMWALCOHOL) ANlMAt NOMBER BODYW. (GRAM) BRAIN HEART 17 3- 2-_2 is 295 19 312 20 299 21 315 22 3 48 23 313 24 312 0.62 0.357 0.68 0.441 0.64 0.300 0.65 0.345 0.58 0.358 0.59 0.376 0.60 0.311 0.62 0.398 2.83 3.24 2.99 3.16 3.46 3..18 3.40 3.27 0.65 0.78 0.69 0.65 0.84 . 0.63 0.75 0.86 0.013 0.153 0.95 0.015 0.170 0.84 0.011 0.162 0.95 0.015 0.179 0.94 0.017 0.161 0.96 0.013 0.224 0.86 0.015 0.218 0.86 0.016 0.201 0.94 LIVER 3.15 3.97 3.11 3.18 3.33 3.08 3.24 3.34 KmhlGls ADRmALs S P m m TESTES oii 0.73 0.015 0.144 0.95 0.87 0. 0.187 1.11 0.75 0.017 0.173 1.02 0.75 0.018 0.181 1.07 0.76 0.016 0.179 0.83 0.74 0.014 0.205 0.91 0.68 0.023 0.179 0.92 0.67 0.016 0.189 0.95 33 3x3 34 288 35 293 36 293 ' 37 303 38 324 39 319 40 304 0.63 0.67 0.66 0.66 0.67 0.61 0.62 0.60 0.360 0.327 0.386 0.332 0.436 0.333 0.338 0.369 3.16 3.08 2.97 3 -04 3.19 3.21 2.97 2.94 0.66 0.75 0.71 0.73 0.83 0.73 0.68 0.70 0.017 0.016 0.013 0.020 0.019 0.017 0.014 0.018 0.204 0.173 0.162 0.181 0.199 0.183 0.206 0.180 0.80 0.96 0 -95 0.94 0.94 0.92 0.84 0.97 49 290 50 296 5--1 3-4-2- 52 324 53 320 54 330 55 284 56 263 0.67 0.70 0-.6.-2~ 0.61 0.62 0.60 0.69 0.73 0.364 2.84 0.403 3.09 0.338 3.17 0.378 2.94 0.416 2.93 0.366 2.89 0.361 2.99 0.397 2.73 0.69 0.016 0.168 1.10 0.70 0.011 0.212 1.01 0.74 0.013 0.210 0.81 0.70 0.016 0.173 0.91 0.74 0.013 0.197 0.81 0.68 0.012 0.166 0.82 0.73 0.016 0.189 1.04 0.66 0.022 0.197 1.05 284 NOTOX, Project 242933 --.C. I ORGANIBODY WEIGH" RATIOS (%) AFTER 4 WEEKS MALES G R O U P 5 (PFHS) AMMAL NUMBER BODYW. (GRAM) BRAIN HEART LIVER 6--5 -3-5-2 66 270 67 297 68 297 69 287 7.0_ 347 71 311 72 306 0_ .5_ 7 0.70 0.68 0.65 0.65 0.60 0.59 0.67 0.360 0.367 0.381 0.387 0.382 0.332 0.331 0.340 4.79 4.27 4.67 4.41 4.52 4.18 3.81 5.10 GROUP 6 (PFOS) BACK TO MAIN - OW-IND 4 30 -MAR-99 . KIDNEYS ADRmALs SPLEEN TESTES 0.79 0.79 0.84 0.78 0.73 0.71 0.76 0.74 0.011 0. oi4 0.020 0.015 0 * 020 0.016 0.016 0.018 0.1_ 69 . 0.187 0.185 0.182 0.199 0.2-1~5~ 0.166 0.176 0.91 0.31 1.07 1.03 0.98 0.92 0.98 0.99 81 3 03 82 295 83 3 08 84 318 85 362 86 293 87 351 88 317 0.66 0.364 0.67 0.395 0.67 0.312 0.63 0.368 0.54 0.338 0.66 0.430 0.56 . 0.331 0.65 0.317 3.64 3.79 3.46 3.62 3.72 4.40 3.51 3.53 0.71 0.7.5 0.62 0.76 0.65 0.78 0.64 0.62 0.013 0.019 ` 0.013 0.010 0.017 0.013 0.019 0.013 . 0.165 0.147 0.182 0.160 0.191 0.227 0.181 0.148 0.92. 0.97 0.91 0.88 0.80 0.88 0.92 0.90 285 NOTOX Project 242933 -- Le BACK TO MAIN - OW-IND 5 3 0-MAR- 99 ANIMAL NUMBER 97 _98_ 99 100 101 102 103 104 BODYW. 201 -1-94- 204 217 241 229 183 242 BRAIN 1.93 _ 1.92. 1.89 1.96 1.91 1.89 1.70 1.85 HEART 0.861 0.799 0.750 0.869 0.917 0.901 0.855 0. a67 LIVER 6.95 6.34 6.13 6.82 7.19 6.61 5.79 7.13 GROUP 2 ("ELOMEFt ALCOHOL) ANIMAL NUMBER BODYW. BRAIN HEART 113 204 114 246 115 215 116 218 117 194 118 200 119 201 12 0 213 1.91 2.03 1.92 2.08 1.82 1.89 1.93 1.88 0. a90 0.883 0.785 0.827 0.749 0. a29 0.751 0.786 LIVER 6.96 8.13 7.39 7.59 6.97 6.78 6.35 7.29 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER mmw. BRAIN --- 129 204 13 0 182 1.84 13 1 132 i 133 196, 205 181 1.86 1.88 1.77 13 4 215 1.92 13 5 227 1.85 13 6 193 1.79 HEART --- 0.744 0.736 0. a47 0.770 0.799 0.917 0.714 LIVER --- 5.70 6.48 6.23 6.19 6.84 7.07 6.09 G R O U P 4 ( ,) ANmAL MJMBER BODYW. BRAIN 145 193 1.80 1_ 6_6_ 222 1.89 147 2.01 148 1.84 149 220 1.96 150 219 2.00 iii 200 1.84 152 201 1.85 HEART 0.794 0.961 0.794 0.767 0.855 0.773 0.778 0.719 LIVER 6.44 6.76 6.05 5.72 8.10 6.71 6.22 6.28 I(IDNEys AmmALs SPLEEN OVARIES 1.44 0.060 0.424 0.089 1.39 0.075 0._ 34_5. _ 0.1_42_ 1.35 0.052 0.398 0.128 1.56 0.057 0.483 0.119 1.71 0.046 0.458 o.l.30 1.49 0.065 0.366 0.163 1.36 0.062 0.426 0.100 1.71 0.061 0.481 0.122 IalNEYs ADREMLLS SPLEFN OVARIES 1.47 1.85 1.75 1.44 1.68 1.59 1.48 1.75 KImEYs 0.055 0.056 0.078 0.062 0.073 0.054 0.067 0.069 0.470 0.479 0.501 0.378 0.433 0.435 0.446 0.457 - SPLEEN --- --e --- 1.42 0.052 0.298 1.61 0.053 0.300 1.59 0.060 , o .479 1.36 0.054 0.454 1.57 0.063 0.494 1.62 0.077 0.487. 1.41 0.060 0.409 0.129 0.130 0.123 0.121 0.172 0.144 0.104 0.142 OVARIES --- 0.127 0.162 0 .U8 0.125 0.127 0.146 0.118 xIDNExs ADRFNAts SPLEm OVARIES 1.53 1.46 i.64 1.50 1.89 1.48 1.41 1.33 0.066 0.160 0.057 0.043 0.055 0.068 0.049 0,061 1.527 0.529 0.511 0.506 0.420 0.478 0.463 0.429 0.131 0.055 0.138 0.116 0.156 0.130 0.136 0.129 286 NOTOX P r o j e c t 242933 BACK TO MAIN - OW-IND 6 30-MAR-9 9 161 193 162 201 163 191 164 202 165 198 166 208 167 183 168 228 1.94 1.93 1.78 1.78 1.81 1.98 1.86 1.99 0.769 0.703 0.816 0.800 0.910 0.881 0.688 0.880 7.96 6.70 5.97 6.57 7.08 6.67 5.68 7.08 1.61 1.42 1.32 1.68 1.46 1.48 1.47 1.62 0.068 0.056 0.060 0.047 0.071 0.062 0.049 0.052 0.436 0.419 ' 0.397 0.443 0.361 0.431 0.357 0.415 0.150 0.102 0.121 0.138 0.138 0.152 0.115 0.141 177 215 178 163 185 173 185 199 183 191 184 188 1.86 0.788 9.28 1.69 0.053 0.474 0. I11 1.79 0.660 6.47 1.29 0.044 0.327 0.135 1.91 0.663 7.23 1.48 0.047 0.386 0.108 1.86 0.673 7.16 1.41 0.061 0.394 0.115 1.88 0.818 7.62 1.39 0.055 0.404 0.146 1.96 0.845 8.41 1.47 0.059 0.420 0.110 1.91 0.777 7.85 1.43 0.059 0.442 0.154 1.88 0.757 8.36 1.52 0.057 0.450 0.148 287 NOTOX Project 242933 -C L ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEERS FEMALES GROTJP 1 (VEKICLE CONTROL) -- BACK TO MAIN - OW-IND 7 3 0-MAR-9 9 97 201 98 194 99 204 100 217 101 241 102 229 103 183 104 242 0.96 0.428 0.99 0.412 0.93 0.368 0.90 0.400 0.79 0.380 0.83 0.393 0.93 0.467 0.76 0.358 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER Booyw. 1-( BRAIN HEART 113 204 114 246 115 215 116 218 117 194 118 200 119 201 120 213 0.94 0.83 0.89 0.95 0.94 0.95 0.96 0.88 0.436 0.359 0.365 0.379 0.386 0.415 0.374 0.369 3.46 3.27 3.01 3.14 2.98 2.89 3.16 2.95 LIVER 3.41 3.31 3.44 3.48 3.59 3.39 3.16 3.42 0.71 0.71 0.66 0.72 0.71 . 0.65 0.74 0.71 0.030 0.039 0.025 0.026 0.019 0.028 0.034 0.025 0.211 0.1780.195 0.223 0.190 0.160 0.233 0.199 0.044 0.677 0.063 0.055 0.054 0.071 0.055 0.050 KIDNEYS ADRENALS SPLEEN ovARn?s 0.72 0.027 0.75 0.023 0.81 0.036 0.66 0.028 0.87 . 0.038 0.80 0.027 0.73 0.033 0.82 0.032 0.230 0.195 0.233 0.173 0.223 0.218 0.222 0.215 0.063 0.053 0 .OS7 0.056 0.089 0.072 0.052 0.067 ANIMAL NUMBER BODYW. (GRAM) 129 204 130 182 131 196 132 205. 13 3 181 134 215 135 227 U6 193 BRAIN --- 1.01 0.95 0.92 0.98 0.89 0.81 0.93 HEART --- 0.409 0.376 0.413 0.425 0.372 0.404 0.370 LIVER --- 3.13 3.31 3.04 3.42 3.18 3 .ll 3.16 KlzhlEys --- 0.78 0.82 0.78 0.75 0.73 0.71 0.73 AaRENALs SPLEEN OVAnEs --- 0.029 0.027 0.029 0.030 0.029 0.034 0.031 --- 0.164 0.153 0.234 0.251 0.230 0.215 0.212 --- 0.070 0.083 0.067 0.069 0.059 0.064 ' 0.061 145 Y33 146 222 ,147 223 148 186 149 220 150 219 151 200 152 201 0.93 0.411 3.34 0.79 0.034 0.791 0.068 0.85 0.433 3.04 0.66 0.072 0.238 0.025 0.90 0.356 2.71 0.73 0.026 0.229 0.062 0.99 0.412 3.07 0.80 0.023 0.272 0.062 0.89 0.389 3.68 0.86 0.025 0.191 0.071 0.91 0.353 3.06 0.68 0.031 0.218 0.059 0.92 o .3a9 3.11 0.70 0.025 0.232 0.068 0.92 0.358 3.12 0.66 0.030 0.213 0.064 288 NOTOX Project 242933 --c BACK TO MAIN - OW-IND 8 30-MXR-99 161 193 162 201 163 191 164 202 165 198 166 208 167 183 168 228 ,1.01 0.96 0.93 0.88 0.92 0.95 1.01 .0.87 GROUP 6 (PPOS) ANIMAL NUMBER WDYW. (GRAM) BRAIN 177 215 0.86 178 163 1.10 179 185 1.03 180 173 1.07 . 181 185 1.02 182 199 0.99 183 191 1.00 184 188 1.00 0.398 0.350 0.427 0.396 0.460 0.424 0,376 0.386 HEART 0.367 0.405 0.358 0.389 0.442 0.425 0.407 0.403 4.13 3.33 3.13 3.25 3.57 3.21 3.10 3.10 LNER 4.32 3.97 3.91 4.14 4.12 4.23 4.11 4.45 0.83 0.035 0.226 0.078 0.70 0.028 0.208 0.051 0.69 0.031 0.208 0.063 0.83 0.023 0.219 0.068 0.74 0.036 0.182 0.070 0.71 0.030 0.207 0.073 0.80 0.027 0.195 0.063 0.71 0.023 0.182 0.062 KIrwEYs ADREMlts SPLEESI OVARIES 0.79 0.025 0.79 0.027 0.80 ' 0.025 0.82 0.035 0.75 0.030 0.74 0.030 0.75 0.031 0.81 0.030 0.220 0.201 0.209 0.228 0.218 0.211 0.231 0.239 0.052 0.083 0.058 0.066 0.079 0.055 0.081 0.079 289 NOTOX Project 242933 - --e ORGANWEIGHTS (GRAM) . .AFTER 2 WEEKS RECOVERY MALES GROUP 1 (VEBXCLE C O r n O L ) RNIMAL NUMBER BODYW. BRAIN HEART 9 344 10 277 11 338 2.03 1.81 2.01 1.185 ~~~ 0.960 1.128 . LIVER 8.98 7.42 8.32 GROUP 2 (TEZOMER ALCOHOL) BACK TO MAIN - OW-IND 9 30 -MAR- 99 KIDNEYS ADRRGus SPLEEN TESTES 2.02 0.039 0.440 2.88 1.74 0.037 0.358 2.55 2.35 0.050 0.526 3 .Q1 25 328 26 295 27 320 1.93 1.007 8.62 1.96 1.068 7.54 2.05 1.112 8.65 GROUP 3 (TEw)MER ACFtY-LArn) ANlMAt NUMBER BODYW. BRAXN HEART 41 379 42 319 43 334 2.01 2.04 1.80 1.1l3 1.284 1.119 LIVER 12.10 8.88 8.71 GROUP4 ( \ 2.04 0.041 0.578. 2.67 2.11 0.046 0.571 2.62 2.09 0.031 0.555 . 1.10 IumEYs ADRmms SPLEEN TESTES 2.75 0.047 0.504 3.16 2.15 0.036 0.578 3.19 2.33 0.047 0.547 2.91 57 415. 58 354 59 329 2.13 2.02 1.91 1.125 1.179 1.155 11.41 10.45 9.05 2.56 2 .50 2.11 0.041 0.063 0.062 0.739 0.582 0.548 3.53 3.35 3.18 ANIMAL NUMBER 73 74 75 BODYW. 386 249 336 BRAIN 2.00 1.73 2.06 HEART 1.309 1.027 1.112 LIVPC 12.15 6.89 9.62 KIDNEYS ADRmArs SPLEm TESTES 2.54 0.045 0.630 3.27 1.60 0.035 0.476 1.34 2.41 0.043 0.517 3.21 NOTOX P r o j e c t 242933 ORGANWEIGaTS (GRAM) . AFTER, 2 WEWS RECOVWY HALES GROUP 6 (PFOS) ANR4AL NOMBER BODY W. BRAIN HEART 89 394 90 370 91 377 2.03 1.333 2.07 1.237 2.00 1.328 -- BACK TO MAIN - OW-IND 10 30-MAR-99 LIVER IamEYs ADRENALS SPLEEN 14.20 2.66 14.62 - 2.58 13.39 2.70 0.041 0.048 0.054 -0 758 0.631 0.517 TESTES 3- -31 2.80 2.97 291 NOTOX Project 242933 ---.e BACK TO MAIN - ON-IND 1 1 3 0-MAR-9 9 GROUP 1 (VeElr-rW CONTROL)' ANSMAG NUMBER mmw. 1-( BRAIN HEIRT 9 344 10 277 11 338 0.59 0.344 0.65 0.347 0.60 0.334 LIVER 2.61 2.68 2.46. GROUP 2 (TELOMWALCOHOL) KIDNEYS nDRmmrJs SPLEEN TESTES 0.59 0.011 0.128 0.84 0.63 o.ol3 0.129 0.92 0.70 0.015 0.156 0.89 25 328 26 295 27 320 0.59 0.307 2.63 0.62 0.013 0.176 0.81 0.67 0.362 2.56 0.72 0.016 0.194 0.89 0.64 0.348 2.70 0.65 0.010 0.173 . 0.34 41 379 . 0.53 42 319 0.64 43 334 0.54 0.294 3.19 0.403 2.78 0.335 2.61 0.73 0.012 0.133 0.83 0.67 0.011 0.181 1.00 0.70 0.014 0.164 0.87 57 415 ' 0.51 0.271 2.75 0.62 0.010 0.178 0.85 58 354 59 329 0.57 0.333 2.95 0.71 0.018 0.164 0.95 0.58 0.351 2.75 0.64 0.019 0.167 0.97 73 386 74 249 75 33 6 0.52 0.339 3.15 0.66 0.012 0.163 , 0.85 0.69 0.412 2.77 0.64 , 0.014 0.191 0.54 0.61 0.331 2.86 0.72 0.013 0.154 0.95 292 NOTOX Project 242933 --..- ORGAE;I/BOm WEIGHT RATIOS ( 8 ) AFTEfl: 2 WEEKS RECOVERY I!!IAIZs: GROUPi 6 (PFOS) BACK TO MAIN - OW-IND 12 - 30-MA2 9 9 89 394 90 370 91 377 0.52 0.338 3.60 0.68 0.56 0.334 3.95 0.70 0.53 . 0.352 3.55 0.72 0.010 0.013 0.014 0.192 0.171 0.137 0.84 0.76 0.79 293 NOTOX Project 242933 - L ORGANWEIGHTS (GRAM) . AFTW 2 WEEKS RECOVERY FEMALES GROUP 1 (VEEICLE CONTROL) BACK TO MAIN - OW-IND 13 30-MAR-9 9 105 217 106 272 107 227 1.98 0.867 6.95 1.51 0.052 0.449 0.150 2.05 0.960 9.23 1.71 0.056 0.439 0.574 1.85 0.868 6.90 1.39 0.057 0.372 0.l32 G R O W 2 (TELOMER ALCOHOL) 121 200 122 203 123 231 1.85 1.85 1.98 0.812 0 * 915 0.848 6.72 6.01 7.72 GROUP 3 (TELOMER ACRYLATE) ANIMAL m BODYW. BRAIN HEART 137 217 13 8 189 13 9 200 1.88 1.91 1.94 0.873 0.767 0.853 LrvER 7.40 5.66 6.06 GROUP 4 ( I ) 1.60 0.074 0.367 0.125 1.31 0.057 0.370 0.135 1.62 0.057 0.536 0.131 KImEYs ADREMlts SPLEEN OvxUEs 1.70 0.080 0.523 0.120 1.41 0.047 0.382 0.120 1.44 0.067 0.344 0.138 153 214 ' 154 246 155 191 1.89 1.95 1.90 0.980 0.950 0.878 7.05 ,7.00 7.18 ~ 1.61 1.72 1.48 0.075 0.065 0.068 0.604 0.432 0.405 0.149 0.162 0.123 169 228 1.90 0.844 6.77 1.54 0.061 0.362 0.135 170 211 1.92 0.784 6.84 1.55 0.055 0.508 0.118 171 23 2 l.95 0.892 7.96 1.45 0.059 0.476 0.147 294 NOTOX Project 242933 ----.e OF"wEIGH!rS (GRAM) . AFTER 2 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) ANfMAt NUMBER BODYW. BRAIN HEART 185 197 2.02 0.702 186 -199 1.93 0.735 187 203 2.04 0.732 LIVER 8.18 7.10 7.37 BACK TO MAIN .. - OW-IND 14 30-MAR- 0 9 KIceJcls ADRmaLs SPLEEN OvARizS 1.48 1.41 1.26 0.057 0.056 0.055 0.397 0.415 0.365 0.138 0.132 0.127 295 NOTOX Project 242933 -- & ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER B O D Y W . BRAIN (GRAM) ' HEART LIVER 105 217 106 272 107 227 0.91 0.400 3.20 0.75 . 0.353 3.39 0.01 0.382 3.04 .. c BACK TO MAIN - OW-IND 15 30 -MAR- 99 KIDNEYS ADREMLts SPLEm OVARIES 0.69 0.024 0.207 0.069 0.63 0.021 0.161 0.211 0.61 0.025 0.164 0.058 ANIMAt NUMBER BODYW. (GRAM) 121 200 122 203 123 231 BRAIN 0.93 0.91 0.86 HEART 0.406 0.451 0.367 LNER KImEYs ADRENACS SPLEEN OVARIES 3.36 0.80 2.96 0.65 3.34 ' 0.70 0.037 0.028 0.025 0.184 0.192 , 0.232 0.063 0.067 0.057 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODYW. (GRAM) BRAIN HEART LIVER 137 217 13 a 189 13 9 200 0.87 1.01 0.97 0.402 3.41 0.406 3.00 0.427 3.03 laSaJcr.5 ADRENAm SPLEm ovauEs 0.79 0.037 0.241 0.055 0.75 0.025 0.202 0.063 0.72 0.034 0.172 0.069 GROUP 4 ( - :) ANIMAL NUMBER BODYW. (GRAM) BRAIN 153 214' 154 246 155 191 0.88 0.79 0.99 HEART 0.458 0.386 0.460 LIVER 3.29 2.85 3.76 KIDNEYS ADRENAm s!?LEm OVARIES 0.75 0.70 0.77 0.035 0.026 0.036 0.189 0.176 0.212 0.070 0.066 0.064 169 a2a 170 211 171 232 0.83 0.370 2.97 0.91 0.372 3.24 0.84 0.384 3.43 0.68 0.74 0.62 ' 0.027 0.026 0.025 0.159 0.241 0.205 0.059 0.056 0.063 296 NOTOX P r o j e c t 242933 - C& ORGAN/BODY WEIGHT RATIOS (%) A F T W 2 WEEKS RECOVERY FEMALeS GROUP 6 (PFOS) BACK TO MAIN - OW-IND 1 6 - 3 0 -MAR 99 185 197 186 199 187 203 1.02 0.356 4.15 0.75 0.029 0.202 0.070 0.97 0.369 3.57 0.71 0.028 0.209 0.066 1.00 0.361 3.63 0.62 0.027 0.180 0.063 297 BACK TO MAIN NOTOX Project 242933 - . I ORGANWEIGHTS (GRAM) - AFTER 4 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODYW. BRAIN HEART 13 415 14 360 15 392 1.97 1.284 1.83 1.206 2.u 1.099 LIVER 12.26 9.57 10.47 . .. - - OW-IND 1 7 30 -MAR 99 KIDNEXS ADRzNXs SPLEEN l?zsTEs 2.69 0.038 0.576 2.86 2.38 0.041 0.611 2.73 2.24 0.058 0.656 3.01 GROUP 2 (TELOMER ALCOHOL) ANDGUl B o o y w . BRAIN HEART LIVER KIsmYs ADRmus SPLEEN l%sTEs \ kxM0ER --- --- --- --- --- --- --- --- I1 29 330 2.01 1.195 9.41 2.35 0.048 0.456 2.97 30 396 2.08 1.199 10.27 2.48 0.059 0.544 2.92 I : 31 3 62 2.08 1.228 9.66 2.44 0.042 0.620 3.68 32 GROUP 3 (TExmmR ACRYLATE) ANPIAL NUMBER BODYW. BRAIN HEART 45 380 2.07 1.282 46 380 2.07 1.237 47 375. 2.12 1.132 LIVER 11.08 9.77 10.05 KIDNEXS ADRENACS S P I 5 N TEsqs 2.55 0.050 0.656 3.06 2.48 0.056 0.534 3.11 2.50 0.057 0.582 3.17 ANIMAL NUMBER 61 62 63 WDYW. 434 406 363 , BRAIN 2.33 1.99 2.00 HEART 1.378 1.389 1.129 LIVER 9.91 10.46 9.81 KIDNEYS ADREMlcs SPLEEN TESPES 2.79 2.32 2.67 0.070 0.692 0.046 . 0.705 0.051 ' 0.762 3.36 2.97 2.98 77 358 78 321 79 326 2.11 1.95 1.99 1.105 0.875 1.164 9.57 8.43 8.54 2.13 1.89 2.69 0.050 0.045 0.047 0.625 0.529 0.502 3.22 3.03 3.01 298 BACK TO MAIN NOTOX Project 242933 - .& ORGANWEIGarS (GFWd) . AFTER 4 WEEKS RECOVERY MALES GROUP 6 (PFOS) ANIMAL NUMBER BODYW. BRAIN WEART LIVER 93 301 94 374 95 356 1.96 0.953 8.98 2.08 1.a29 12.66 1.98 1.311 11.87 KIDNEYS 2.26 2.66 2.53 .. . ADRFNACS SPLEEN 0.055 0.037 0.047 0.505 0.774 0.607 - OW-IND 1 8 3 0-MAR-9 9 TEsT!Is 3.10 3.62 3.09 j 299 BACK TO MAIN NOTOX Project 242933 --c ORGAN/BODY WEIGRP RATIOS (%) AFTER 4 WEEKS RECOVERY MALES GROTJP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODYW. (GRAM) BRAIN HEART LIVER 13 415 0.47 14 360 0.51 15 392 . 0.54 0.309 0.335 0.280 2.96 2.66 2.67 - OW-IND 19 30-MAR-9 9 laENE!fs ADRmALs SPLEEN lTsrEs 0.65 0.009 0.u9 0.69 0.66 0.011 0.170 0.76 0.57 0.015 0.167 0.77 G R O W 2 (TEfx)MwALCOHOL) ANIMAL NUMBER BODYW. (GRAM) BRAIN HEART LIVER KIcucls AaRENALs SPLEEN T)?srEs -. 29 330 0.61 0.362 2.85 0.71 0.015 0.U8 0.90 30 396 0.52 0.303 2.59 0.63 0.015 0.137 0.74 31 3 62 0.57 0.339 2.67 0.67 0.012 0.171 1.02 45 380 46 3 80 47 375 GROUP 4 ( 0.54 0.55 0.57 1 0.337 0.326 0.302 2.92 0.67 2.57 . 0.65 2.68 0.67 0.013 0.173 0.80 0.015 0.141 0.82 0.015 0.155 0.85 61 434 0.54 0.318 2.28 0.64 0.016 0.159 0.77 62 406 0.49 0.342 2.58 0.57 , 0.011 0.174 0.73 63 3 63 0.55 0.311 2.70 0.74 0.014 0.210 0.82 I -G R O U P 5 (PPHS) iWM4L BODY W. (GRAM) BRAIN HEART LIVER ICIDNEYS ADRENACS SPLEEN T!%srEs 77 358 78 321 79 326 0.59 0.61 0.61 0.309 2.67' 0.59 0.014 0.175 0.90 0.273 2.62 0.59 ' 0.014 0.165 0.94 0.357 . 2.62 0.83 0.014 0.154 0.92 300 NOTOX Project 242933 - --.- ORGAN/BODY WEIGHT RATIOS ( 8 ) AFTER 4 WEWS RECOVERY MALES GROUP 6 (PFOS) ANIMAL NUMBER BODYW. (GRAM) BRAIN HEART LIVER 93 301 94 374 95 356 0.65 0.317 2.98 0.56 0.382 3.39 0.56 0.368 3.34 BACK TO MAIN - OW-IND 2 0 - 3 0-MAR 9 9 .. KIDNEYS ADRmaLs SPLEEN TEsEs 0.75 0.11 0.71 0.018 * 0.010 0.013 0.168 0.207 0.171 1.03 0.97 0.87 .. 301 NOTOX Project 242933 ORGANWEIGHTS (GRAM) AFTW 4 WEEKS RECOVERY FEMALES GROUP 1 (VEBICLE CONTROL) ANmU NIIMBp;R BODYW. BRAIN HEART - 109 212 110 261 111 255 1.86 1.87 2.01 0.847 1.052 0.850 GROUP 2 (TELOMER ALCOHOL) AMMAL NmBm BODYW. BRAIN HEART 125 223 126 220 127 128 -24-1- 2.00 0.826 ---1.89 0.757 2.00 -0-.9- 71 -- BACK TO MAIN - OW-IND 21 30 -MAR-99 LIVER 5.83 8.12 7.09 KImEYs ADRmALs SPLEEN OVARIES 1.34 0.062 0.426 0.137 1.84 0.078 0.508 0.144 1.60 0.055 . 0.402 0.118 LIVER 7.21 6.22 7-.-2-0 KIcNGls ADREMlls SPLEEN OVARIES 1.62 0.079 0.430 0.122 1.72 0.053 0.309 0.138 -1.-5-3 -0-.0-61 -0.-5-89 -0.-1-79 ANIMAL NUMBEX BODYW. 141 2 12 142 211 143 254 BRAIN 1.83 1.86 1.94 HEART 0.854 0.834 0.922 LIVER 6.69 6.09 7.79 IcrmEYs ADRmALs SPLEEN OVARIES 1.64 0.068 0.429 0.160 1.38 0.053 0.489 0.138 1.61 0.066 0.360 0.136 I GROUP 4 ( 1 ANIMAt mER mw. BRAIN HFaRT LIVER KIDNEYS ADRmALs SPLEEN OVARIES 157 219 158 211 159 275 2.02 0.900 6.04 1.46 0.061 0.595 0.125 1.94 0.836 6.82 1.62 0.061 0.391 0.167 1.94 1.018 9.04 1.95 0.062 0.525 0.204 173 219 174 241 175 219 1.85 0.866 6.99 1.75 0.064 0.377 0.123 1.97 0.922 6.88 1.58 ' 0.068 0.329 0.128 1.81 0.793 6.60 1.52 0.063 0.358 0.207 302 NOTOX Project 242933 - -I ORGANWEIGHTS (GRAM) . AFTER 4 WEKS RECOVERY FEMALES G R O m 6 (PFOS) ANlMAt NUMBER BODYW. BRAIN HEART LIVER 189 201 190 220 191 202 1.78 0.854 7.72 1.92 0.799 7.61 1.93 0.823 7.15 BACK TO MAIN .. - OW-IND 22 30-MAR-9 9 KIDNEYS ADRENALS SPLEEN OVARIES 1.55 0.069 0.485 0.139 1.80 0.049 0.431 0.144 1.68 0.055 0.366 0.141 . I .. . I 303 .. NOTOX Project 242933 --c_- ORGAN/BODY WEIGBT RATIOS (%) AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEEICLE CON!rROL) BACK TO MAIN - OW-IND 23 3 0-MAR- 99 109 212 110 261 111 255 0.88 0.71 0.79 0.400 0.403 0.333 GROUP 2 (TELOMES ALCOHOL) ANIMAL NUMBER BODYW. (GRAM) BRAIN HEART 125 223 , 126 220 .. 127 128 2-4-1- 0.96 0.370 0.86 0.344 -0.-8-3 -0.-4-03 2.75 3 .ll 2.78 LIVER 3.23 2.83 -2.-9-9 0.63 0.029 0.70 0.030 0.63 0.022 KICNEYS AD- 0.73 0.035 0.70 0.024 -0.-6-4 -0.-0-25 0.201 0.195 0.158 0.065 0.055 0.046 S P L E Z N , OVARIES 0.193 ---0.140 0.244 0.055 ---0.063 0.074 141 212 142 211 143 254 0.87 0.403 3.15 0.77 0.032 0.202 0.075 0.88 0.395 2.89 0.66 0.025 0.232 0.065 0.76 '0.363 3.07 0.63 0.026 0.142 0.054 157 219 1. , 150 211 159 275 0.92 0.411 2.76 0.67 -0.020I 0.272 0.057 0.92 0.396 3.23 0.77 0.029 0.185 0.079 0.10 0.370 3 -29 0.71 , 0.023 0 f 191 0.074 173 219 114 241 175 219 0.85 0.395 3.19 0.80 0.029 0.172 0.056 0.82 0.383 2.86 0.65 0.020 0.137 0.053 0.83 0.362 3.02 0.69 0.029 0.163 0.095 304 NOTOX Project 242933 -L1 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEKS RECOVERY FEMALES GROUP 6 (PFOS) BACK TO MAIN - OW-IND 24 30 -MAR- 99 189 201 190 .220 191 202 0.88 0.87 0.96 0.425 3.84 0.363 3.46 0.407 3.54 o .77 0.82 0.83 0....0.3_ 4 0.022 0.027 0..241 0.196 0.181 0- .-0-6-9- 0.065 0.070 305 ProPath GmbH APPENDIX 1 BACK TO MAIN '.NOTOX Safety El Environmental Research RV. Pathology Report Exploratory 28-Day Oral Toxicity Study with Telomer Alcohol, Telomer Acrylate, , PFHS and PFOS (positive control) by Daily Gavage in the Rat followed by a 14/28-Day Recovery Period NOTOX Project 242933 NOTOX Substances83484,83493,83502,84456and 84141 3M Test no. T-7023, T-7024, T-7022,T-7032, T-6295 This report contains 146 pages Dr. J.Th Wason Obarernattstr.52a Pralteln 4133 Swiherland tek +4i 81 823 04 10 W + 4 1 61 8230411 BACK TO MAIN ... PATHOLOGY REPORT T e s t A r t i c l e : TELCMER ALCOHOL/TELOMER ACRYLATE/ /PMS/PFOS T e s t System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology S e r v i c e s ~ ~~~ Page 2/146 . NOTOX no : 242933 .. Piopath no 99010 Date : 21.Apr. 1999 ~ T a b l e of Contents Table of contents .............................................................. 2 Authentication .................................................................. 3 Summary ........................................................................ 4 Materials and methods .......................................................... 4 Results ........................................................................ 4 Conclusions .................................................................... 4 Incidence table .macroscopic findings ......................................... 4 Incidence t a b l e .s e l e c t e d microscopic findings with grades .................... 4 Incidence table .microscopic findings ......................................... 4 Individual animal microscopic findings ......................................... 4 Animal data list ............................................................... 4 Individual animal data records ................................................. 4 BACK TO MAIN PA!J!IOLOGY REPORT --c_- Test Article: TELOMER ALCOHOLITELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 3/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Authentication The undersigned hereby declares, that the histopathology data in this report were compiled by him, and that they reflect accurately the primary data records. This . report, GmbH consisting of 146 pages, was created by the computer system of Propath Jeffrey Th. Wilson, Dr.med.vet, BVSc, MSc, MRCVS Toxicologic Pathologist Propath QnbII CH-4133 Pratteln, Switzerland BACK TO MAIN --c PATHOLOOY REPORT T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ .I'PEUS/BFOS T e s t System : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services Page 4/146 NOTOX no. : Piopath no.: Date : 242933 99010 21.Apr.1999 summary P'athomorphologic examination was performed on 168 Sprague Dawley rats (84 m a l e s , 84 females) which had been subjected t o an e x p l o r a t o r y 28-day o r a l (gavage) t o x i c i t y s t u d y w i t h t h e test a r t i c l e s T d o n m r alcohol/ Telomsr acrylate/ -/ p m s / PFOS. The r a t s were assigned to' s i x dose groups each c o n t a i n i n g f o u r t e e n males and f o u r t e e n females. T e s t a r t i c l e s were a d m i n i s t e r e d once d a i l y by gavage a t d o s e s of 30 (telomer alcohol, telomer acrylate and ), 1 0 (PFHS') and 3 mg/kg (PFOS)( d o s e groups 2, 3, 4, 5 and 6 r e s p e c t i v e l y ) f o r 28 days. The r a t s of the c o n t r o l group 1 received t h e v e h i c l e , 1%aqueous carboxymethyl c e l l u l o s e , a l o n e . A t t h e end of t h e t r e a t m e n t p e r i o d e i g h t animals of each sex from a l l groups were k i l l e d and subjected t o complete necropsies (main study). Three males and t h r e e females from each dose group were s u b j e c t e d t o necropsy f o l l o w i n g 14-day o r 28-day treatment-free p e r i o d s (recovery a l l o c a t i o n 1 and 2 ) . All r a t s w e r e n e c r o p s i e d . H i s t o p a t h o l o g i c examination was performed on a d r e n a l - glands, kidneys, l i v e r , lymph nodes mandibular and mesenteric, ovaries, pancreas, spleen, testes, thymus and a l l organs o r t i s s u e s with macroscopic . f i n d i n g s All animals s u r v i v e d t h e i r a l l o c a t e d t r e a t m e n t p e r i o d s , H e p a t o c e l l u l a r hypertrophy was p r e s e n t i n group 2 (30 mg/kg/day telomer a l c o h o l ) , group 5 (10 mg/kg/day PFHS) males and i n b o t h s e x e s of group 6 (3 mg/kg/day PFOS) a t t h e end o f t h e main study. I n t h e p a n c r e a s a s l i g h t i n c r e a s e i n t h e i n c i d e n c e o f e x o c r i n e a p o p t o s i s / s i n g l e cell necrosis was recorded i n group 2 males. Following t h e recovery p e r i o d s , t h e h e p a t o c e l l u l a r hypertrophy had undergone p a r t i a l o r complete r e s o l u t i o n i n group 2 and 5 males and i n group 6 females. The i n c i d e n c e of p a n c r e a s a p o p t o s i s / s i n g l e c e l l n e c r o s i s i n group 2 males had a l s o returned t o t h e level of t h e vehicle control group. BACK TO MAIN PATHOLOGY REPORT Test Article: TELWR ALCOHOL/TELOMEACRRYLATE/. - ,/eFHs/pFos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 5/146 NOTOX no. : 242933 Piopath no.: 99010 Date : 21.Apr. 1999 Materials and Methods Study Design Dose Dose group m g / W ~ Number of rats males fdes main males rec.1 Animal numbers tec.2 females m a i n rec.1 rea.2 ,. . . I 1 Vehicle control 2 Telomes 30 alcohol ' 3 Telomes 30 rcrylate 4 30 I!?EL lo I so:, 8 3/3 8 3/3 1 8 9-11 13-15 97-104 105-107 109-111 8 3/3 8 3/3 17-24 25-27 29-31 113-120 121-123 125-127 8 3/3 8 3/3 33-40 . 41-43 45-47 129-136 137-139 141-143 8 3/3 8 3/3 49-56 57-59 61-63 145-152 153-155 157-159 8 3/3 8 3/3 65-72 73-75 77-79 161-168 169-171 i73-175 8 3/3 8 3/3 81-88 89-91 93-95 177-184 185-187 189-191 Administration of the T e s t Article .The test article was administered daily by gavage for 28 days. The recovery group rats were allowed a 14-day (recovery 1) or 28-day (recovery 2) treatment free period. Rats of the control group 1 received the vehicle, 1% aqueous carboxymethyl cellulose, alone. Necropsy and Histopathology A t the end of the assigned study periods, the rats were killed by exsanguination following anesthesia by ether vapour. Complete necropsies were performed on all rats. Representative tissue samples of the following organs were preserved in 4% _ .8' phosphate buffered neutral formaldehyde solution (10% formalin). Adrenal glands, aorta, bone - sternum and femur including joint; bone marrow - - - sternal, brain, clitoral glands, epididymides, esophagus, eyes with optic nerve and Harderian glands; heart, kidneys, lacrimal glands - exorbital, large intestine cecum, colon and rectum; larynx, liver, lungs, lymph nodes mandibular and mesenteric; female mammary gland area, nasopharynx, ovarie.9, pancreas, pituitary gland, preputial glands, prostate gland, salivary glands - mandibular and sublingual; sciatic nerve, seminal vesicles, skeletal muscle, - skin, small intestine duodenum, jejunum and ileum with Peyer's patches; spinal - cord cervical, Inidthoracic and lumbar; spleen, stomach, testes, thymus, thyroid glands with parathyroid glands, tongue, trachea, urinary bladder, uterus with uterine cervix, vagina and all organs or tissues with macroscopic a b n O ~ l i t i e S - BACK TO MAIN - PA"H0LOGY REPORT T e s t Article: TELCMER ALCOHOLITELOMER ACRYLATE/ / e m w p m T e s t System : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3~ Toxicology S e r v i c e s Page 6/146 NOTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Materials and Methods Following f i x a t i o n , t h e f o l l o w i n g organs from a l l animals, were trimmed, p r o c e s s e d and embedded i n p a r a f f i n wax. S e c t i o n s w e r e c u t a t a t h i c k n e s s of 2-4 micrometers and s t a i n e d w i t h hematoxylin and e o s i n . Numbers of s e c t i o n s made a r e given i n parentheses. - Adrenal: glands ( 2 ) , kidneys ( 2 ), l i v e r (21, lymph nodes mandibular ( 2 ) and m e s e n t e r i c (I), o v a r i e s (21, p a n c r e a s (11, s p l e e n (11, testes (21, thymus (1) and a l l o r g a n s or t i s s u e s w i t h macroscopic f i n d i n g s . . I n i t i a l l y s e c t i o n s of t h e above organs from a l l main atudy r a t s were examined by l i g h t microscopy. Following t h i s examination, s e c t i o n s of liver f r o m g r o u p s 1 ( v e h i c l e c o n t r o l ) , 2 ( t e l o m e r a l c o h o l ) , 5 (PFHS) and 6 (PFOS) males and g r o u p s 1 and 6 females recovery allocations 1 and 2 were examined. S e c t i o n s o f pancreas f r o m group 1 and 2 males of recovery a l l o c a t i o n s 1 and 2 were a l s o examined. The s e c t i o n s w e r e i n February/March, 1999. Data Compilation The animal d a t a and macroscopic f i n d i n g s were e l e c t r o n i c a l l y t r a n s f e r r e d from t h e n e c r o p s y raw d a t a f i l e s of NOTOX i n t o t h e computer system of Propath GmbH where t h e m i c r o s c o p i c f i n d i n g s w e r e r e c o r d e d by t h e undersigned p a t h o l o g i s t u s i n g online input Macroscopic f i n d i n g s are p r e s e n t e d i n summary i n t h e table " I n c i d e n c e o f Macroscopic Findings" and i n f u l l d e s c r i p t i v e terms i n t h e "Individual Animal Data Records". Wherever p o s s i b l e , macroscopic f i n d i n g s were c o r r e l a t e d w i t h a microscopic finding. Microscopic findings a r e listed f o r each animal along with severity grades i n t h e table " I n d i v i d u a l Microscopic F i n d i n g s " and summarized i n t h e t a b l e s " I n c i d e n c e of A l l Microscopic Findings" and "Incidence T a b l e - Selected Microscopic Findings w i t h Grades". They a r e f u r t h e r g i v e n i n f u l l d e s c r i p t i v e terms i n t h e " I n d i v i d u a l Animal Data Records". H i s t o l o g i c changes w e r e described according t o t h e i r d i s t r i b u t i o n , severity, and morphologic character. BACK TO MAIN - -c PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMERACRYLATE/ Jems/eFos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 7/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Results Mortality A iemale group 3 rat, no. 12% L 3 d at blood sampling on the day of scheduled sacrifice. All other rats survived their assigned study periods. Macroscopic Findings The few macroscopic findings recorded at a l l aacrifices were unremarkable and did not distinguish treated rats from'the vehicle control group. Microscopi c Findings Treatment related microscopic findings were noted in the liver and pancreas. A t the end of the main study, midzonal/centrilobular hypertrophy was seen at a minimal or slight degree in six group 2 (telomer alcohol) males, minimal to moderate degrees in all group 5 (PFHS) males and all group 6 (PFOS) rats of both sexes. By the end of the 28-day recovery period (recovery 2)this finding had partially or completely resolved in group 2 and 5 males and in group 6 females, however remained in evidence in group 6 males. In the pancreas a slight increase in the incidence of minimal or slight degrees of exocrine apoptosis/single cell necrosis was recorded in group 2 males at the end of the main study. The incidence of this finding returned to the level of the vehicle control group following the recovery periods. The remainder of the microscopic findings recorded at both necropsies, were within the range of background pathology encountered in Sprague Dawley rats of this age and strain and occurred at similar incidences and severity in both control and treated rats. BACK TO MAIN PATHOLOGY REPORT - --a Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 8/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Fqr.1999 Conclusions Morphologic alterations following the administration of Telomer alcohol/Telomer acrylate/ /PFHs/PFOS by once daily gavage to Sprague Dawley rats for twenty eight days, were present in the liver of telomer alcohol, PFHS and PFOS treated rats and in the pancreas of telomer alcohol treated rats. Hepatocellular hypertrophy was present in group 2 (30 mg/kg/day telomer alcohol), group 5 (10 mg/kg/day PE'HS) males and in both sexes of group 6 (3 mg/kg/day pros) at the end of the main 8tUdy. In the pancreas a slight increase in the incidence of exocrine apoptosidsingle cell necrosis was recorded in group 2 males. Following the recovery periods, the hepatocellular hypertrophy had undergone partial or complete resolution in group 2 and 5 males and in group 6 females. The incidence of pancreas apoptosis/single cell necrosis in group 2 males had also returned to the level of the vehicle control group. BACK TO MAIN PATHOLOGY REPORT ----.I Test Article: TELOMER ALCOHOL~TELOMERACRYLATE/. ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 91146 NOTOX no. : Prbpath no.: Date : 242933 99010 21.Apr.1999 Inddence T a b l e - Macroscopic Findings M a i l l Study SEX: DOSE GROUP: number of animals Lungs Hemorrhage Stomach Hemorrhage - Liver Enlarged Kidnevs Pelvic dilation .Testes Reduced in s i z e Epididymides - Reduced i n s i z e Ovaries Watery c y s t . -E 123456 888888 0 0 0 0 1, 0 0 0 0010 0 0 0010 0 0 0000 0 0 0010 0 0 0010 ZmfaLE 123456 888888' 000000 000000 000000 010000 100000 000100 BACK TO MAIN PATHOLOGY REPORT - - L Test Article: TELOMER ALCOHOL/TELOMERACRYLATE/ /PFHS/PFos Test System : Exploratory 28-Day Gavage Study in Rats sponsor : 3M Toxicology Services Page 10/146 NOTOX no. : Prdpath no.: Date : 242933 99010 21.Apr.1999 Inddence Table - Macroscopic Findings Recovery Allocation 1 SEX : DOSE GROUP: number of animals - Liver Accentuated lobular pattern - Testes Reduced .in size Skin Alopecia general chest region Scab formation abdominal region MaLE 123456 333333 000001 010010 000000 100000 000000 EmaJz 123456 333333 000000 010000 000000 100000 ~~ BACK TO MAIN PATHOLOGY REPORT - - < e. Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/- /Pms/pms Test System : ExplOKatOIy 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 11/146 NOTOX no. : 242933 Pfopath no.: 99010 Date : 21.Apr.1999 Incidence Table - Macroscopic Findings See- Allocation 2 SEX: DOSE GROUP: number of +mals Eyes Reduced i n s i z e lens m&E 123456 333333 000100 -1 2 3 4 5 6 334333 0 0 0 0 0 0 . I BACK TO MAIN PATHOLOGY REPORT - --.- T e a t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rata Sponsor : 3M Toxicology Services Page 12/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr. 1 9 9 9 Incidence T a b l a - Selected MiCrOSCOpiC Findings w i t h Grades Main Shdy SEX : DOSE GROUP: number of animals - Liver number examined Midzonal/ centrilobular hypertrophy, diffuse biXlfma1) (slight) (moderate) Total Pancreas WLLa. 123456 8 8 8 8 8 8 888888 Rmma 123456 8 8 8 8 8 8 8 8 8 8 8 8 050000 010022 000066 066088 0 0 0 0 0 1 000004 0 0 0 0 0 3 o o o a o 8 number examined Apoptosis/single cell necrosis, exocrine (minfmal) ( s l i g h t) Total 8 8 8 8 8 8 142021 120101 2 6 2 1 2 2 8 8 8 8 8 8 200010 000000 2 0 0 0 1 0 I. I BACK TO MAIN PATHOLOGY BEPORT - .<_- T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PMS/PFOS T e s t System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services Page 13/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr. 1999 Incidence Table - Selected Microscopic Bindings with Grades Ret- Allocation 1 SEX : DOSE GROUP: MaLZ 12'3 4 5 6 RmfALE 123456 number o f animals 3 3 3 3.3 3 333333 Liver number examined 33 33 3 3 Midzonal/ centrilobular hype rtrophy, di fuse (minhl.) 01 10 0 1 (slight) 00 11 0 0 (moderate) 00 02 0 0 Total 01 23 0 1 R~CQT AlJoaation 2 SEX: DOSE GROUP: number of animals Liver number examined Midzonal/ centrilobular hypertrophy, diffuse (minimal .) (slight) . (moder a te) . I Total Pancreas number examined Apoptosis/single c e l l necrosis, exocrine (minimal.) MBLE 123456 333333 33 33 R-EMALE 123456 333333 3 3 01 01 1 00 01 0 0 0 01 0 01 03 1 33 10 BACK TO MAIN PATHOLOGY REPORT - %& Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/. ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology services Page 14/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 , i . I. Incidence Table - Microscopic Findings Maip Study SEX : DOSE GROUP: nunbar of animala Lunqs number examined Intra-alveolar blood, necropsy artefact Vascular m i n e r a l i s a ti o n , focal Stomach 'number examined Liver number examined Inflammatory c e l l foci Single c e l l necrosis Midzonal/ centrilobular hypertrophy, diffuse Hepatocellular vacuolation Hematopoietic cell foci Coagulative necrosis, focal Pancreas number examined Apoptosis/single cell necrosis, exocrine Exocrine atrophy, focal w. 123456 888888 1 1 1 1 888888 8 8 8 8 8 8 666366 060088 100034 300001 000010 888888 262122 011000 EmazE 123456 888888 8 8 8.8 8 8 8 8 8 8 8.8 765354 00000.8 101010 000100 000000 888888 200010 001000 BACK TO MAIN PATHOLOGY REPORT - -L& Test Article: TELOMER ALCOHOLITELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services P a g e 15/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Incidence Table - Microscopic Findings SEX: DOSE GROUP: MAIa 123456 ambar of animala 888888 Pancreas Inflammation, mixed granulocytic/ lymphocytic 000010 Kidneys number examined 888888 Tubular atrophy, cortical 355346 Interstitial inflammation, lymphocytic 312132 Hyaline cast (s) 110202 Tubular mineralization 010000 Pelvic inflammation, lymphoid 001000 Pelvic dilation 000000 Cystic tubules 000011 Pelvic mineralization 010000 Tubular epithelial hypertrophy, focal 000000 Testes number examined 888888 Spennatidic giant cell (s) 100001 Seminiferous tubular atrophy 100010 ., ZmaLE 123456 888888 000000 888888 412105 002103 000000 100000 0 0 0 0 0'0 010000 000110 100000 000010 BACK TO MAIN PATHOLOGY REPORT - --c T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/' -/PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 16/146 NOTOX no. : Prbpath no.: Date : 242933 99010 21 .Apr. 1999 Incidence T a b l e - Microscopic Findings SEX: DOSE GROUP: MLLB 123456 number of animals 8 8'8 8 8 8 Epididymides number examined Atrophy hpda ' - Ovaries number examined Uterus number examined Cyst ( 5 ) Adrenal Glands number examined 888080 Extracapsular nodule 000110 Vacuolation, multifocal i n 2. fasiculata 000002 Atrophy, cortical diffuse 000001 Spleen . . ~ .% number examined Hemopoiesis , primarily erythropoiesis 880888 000008 Hemosiderin pigment 615744 Thymus number examined - Lymphoid atrophy involution 0 a a 0.0 8 .001102 l?mzIz 123456 8 8 8 0 8 8 888888 011011 000000 0 0 0 0 0 0 880888 700505 808808 000080 113111 BACK TO MAIN PATHOLOGY REPORT ----.- T e s t b t i c l e : TELOMER ALCOHOL/TELOMERACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study i r i Rats Sponsor : 3M Toxicology Services Page 17/146 NOTOX no. : Prbpath no.: Date : 242933 99010 21.Apr.1999 Incidence Table - Microscopic Findings SEX : W S E GROUP: MALE 123456 number of animals Thymus 8 8'8 8 8 8 Lymphocyt o l y s i s , increased 001100 Mesenteric Lymph Node number examined 888888 Congestion/ erythrophagocytosis 010100 Mandibular Lmph Node number examined 888888 Plasmacytosis 563242 Lymphoid hyperplasia 200100 Congestion/ erythrophagocytosis 001120 rmA.LE 123456 888888 011110 888888 0 0 0 0 0 0 888888 032112 112310 000000 BACK TO MAIN PATHOLOGY REPORT - -.L Test Article: TELOMER ALCOHOL/PEELOMER ACRYLATE/ JPFHS/PFONSOTOX no. : Test System : Exploratory 28-Day Gavage Study in Rats ' Pmpath no.: Sponsor : 3M Toxicology Services Date : 242933 99010 21.Apr.1999 Incidence Table - Microscopic Findings Rea- Allocation 1 SEX: WSE GROUP: -. 123456 number o f animals - Liver number examined 3 3 3'3 3 3 33 33 Inflammatory c e l l foci 33 33 Single cell necrosis 23 32 Midzonal/ centrilobular hypertrophy, d i f f u s e 01 23 Hepatocellular vacuolation 10 03 Hematopoietic cell foci 01 00 Pancreas number examined 33 Exocrine atrophy, focal 01 Testes number examined 1 1 Seminiferous tubular atrophy - Skin number examined 1 1 Inflammation, exudative ZzUuE 123456 333333 3 3 3 3 2 1 0 1 0 0 11 10 BACK TO MAIN PATHOLOGY REPORT ---...- Test Article: TELCMER ALCOHOL/TELOMER ACRYLATE/.. ,/PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology services Page 19/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Incidence Table - Microscopic Findings Recoveq Ruocation 2 SEX: DOSE GROUP: baLtE. 123456 FE24aLE 123456 number of animals Liver number examined Inflammatory cell foci 333333 333333 33 33 3 3 33 3 3'3 3 Single cell necrosis 22 22 1 2 Midzonal/ 01 03 0 1 cent rilobular hypertrophy, diffuse Hepatocellular vacuolation 10 11 0 Coagulative necrosis, focal 01 00 0. Pancreas number examined Apoptosis/single cell necrosis, exocrine a number examined Periorbital inflammation due to blood sampling 33 10 1 1 BACK TO MAIN PA!CHOLOGY REPORT --c_I T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/' ,/PFHS/PFOS T e s t s y s t e m : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services Page 20/146 NOTOX no. : 242933 Prbpath no.:' 99010 Date : 21.Apr. 1999 Individual. h i n d . Microscopic Findings Codes and symbols in t a b l e heading: rn Males f Females Codes and symbols in animal lines: p Planned s a c r i f i c e (main study) r Recwery 1 (planned sacrifice) s Recwery 2 (planned s a c r i f i c e ) o Other Grading system used i n finding lines: 0 finding not present lminimal 2 slight 3 moderate 4 severe 5 very severe .. x present O n l y organs/groups w i t h findings are l i s t e d i n the table BACK TO MAIN PATHOLOGY REPORT --._- Test Article : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 21/146 NOTOX no. : 242933 Prbpath no.: . 99010 Date : 21.Apr.1999 Individual Animal a c r o s c o p i c Findings I Males, Dose group 1 1 2 3.4 5 6 7 8 m .m m m m m m m necropsy status Liver P P P P P P P P Inflammatory cell foci 2 2 2 2 1 1 2 2 Single cell necrosis 0 1 1 1 3. 1 2 0 Hepatocellular vacuolation 0 0 0 0 0 0 1 0 Hematopoietic cell foci 1 0 1 0 0 0 1 0 Pancreas Apptosis/single cell necrosis, 0 0 1 0 2 0 0 0 exocrine Kidneys T u b u l a r atrophy, cortical 0 0 0 1 0 1 0 1 Interstitial inflammation, lymphocytic 0 0 0 0 1 1 0 1 Hyaline cast (s) Testes 0 0 0 0 0 0 1 0 Spermatidic giant cell (s) 0.1 0 0 0 0 0 0 Seminiferous tubular atrophy .. Spleen 0 1 0 0 0 0 0 0 Hemopoiesis, primarily erythropoiesis 2 2 2 1 1 1 2 1 Hemosiderin pigment Mandibular Lymph Node 0 1 1 1 2 2 1 0 Plasmacytosis Lymphoid hyperplasia 2 2 2'2 0 3 0 0 0 2 2 0 0 0 0 0 BACK TO MAIN PATHOLOGY REPORT - --< Test Article: TELOMER ALCOF~OL/TELOMERACRYLATE/. ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 22/146 NOTOX no. : PrGpath no.: Date : 242933 99010 21.4r.1999 Individual Animal Microscopic Findings Xales, Dose group 1 9 10 11 13 14 15 m .m m m m m necropsy status r r r S s s Liver Inflammatory cell foci 2 3 2' 2 2 2 Single cell necrosis 0 2 1 1 1 0 Hepatocellular vacuolation 0 0 1 1 0 0 Pancreas Apoptosis/single cell necrosis, 0 0 0 0 0 1 exocrine BACK TO MAIN PATHOLOGY REPORT --c- Test Article: TELWER ALCOHOL/TELOMER ACRYLATE/ /PE'HS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 23/146 NOTOX no. : Pr'opath no.: Date : 242933 99010 21.Apr.1999 Individual Anixual Microscopic Findings m a s , Dose group 2 17 18 1 9 - 20 21 22 23 24 m .m m m m m m m necropsy status Liver P P P P P P p .P Inflammatory cell f o c i Single cell necrosis 1 2 1 2 2 2 2 2 1 0 0 1 1 1 1 1 Midzonal/centrilobular hypertrophy, diffuse 0 1 1 1 1 2 0 1 Pancreas Apoptosis/single cell necrosis, 2 1 exocrine Exocrine atrophy, focal 0 0 Kidneys Tubular atrophy, cortical 2 1 0-i 1 1 0 0 Interstitial inflammation, lymphocytic 0 0 0 0 1 0 0 0 Hyaline cast (s) 0 0 0 0 1 0 0 0 T u b u l a r mineralization 0 1 0 0 0 0 0 0 Pelvic mineralization 0 1 0 0 0 0 0 0 ': Spleen Hemopoiesis, primarily erythropoiesis 1 1 Hemosiderin pigment 0 1 Mesenteric Lymph Node Congestion/erythrophagocytosis 0 0 1 0 0 0 0 0 Mandibular Lymph Node Plasmacytosis 2 3 2 0 2 0 2 2 BACK TO MAIN PA!L'HOMGY =PORT --id T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PEOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 241146 NOTOX no. : 242933 Piopath no.: 99010 Date : 21.Apr. 1999 Individual A n i m a l Microscopic Findings Males, Dose group 2 necropsy status Liver 25 26 27 29 30 31 m .m m m m m r r r S s s Inflammatory c e l l foci 2 3 3 2 2 2 Single cell necrosis 1 1 1 0 1 1 Midzonal/centrilobular hypertrophy, diffuse 0 1 0 1 0 0 Hematopoietic c e l l f o d 1 0 0 0 0 0 Coagulative necrosis, focal 0 0 0 0 1 0 Pancreas Exocrine atrophy, focal Testes 0 0 1 0 0 0 Seminiferous tubular atrophy 4 BACK TO MAIN PATHOLOGY REPORT ---- Test Article: TELOMER RLCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 25/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Animal Microscopic Findings W e s , Dose group 3 necropsy status Liver 33 34 3 5 - 36 37 30 39 40 m .m m m m m m m P P P P P P P P Inflammatory cell foci 2 1 Single cell necrosis 1 1 .. Pancreas Apoptosis/single cell necrosis, 1 0 0 0 0 0 1 0 exocrine Exocrine atrophy, focal Kidneys 0 0 0 0 0 0 1 0 Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1. 0 0 1 1 0 1 1 0 1 0 1 0 0 0 0 Pelvic inflammation, lynphoid Spleen 0 0 0 0 0' 0 1. 0 Hemopoiesis, primarily erythropoiesis 2 1 1 1 2 2 2 1 Hemosiderin pigment 1 0 0 0 1 1 1 1 Thymus L' Lymphoid atrophy - involution 0 0 0 0 0 1 0 0 Lymphocytolysis, increased 0 0 0 0 0 0 0 2 Mandibular Lymph Node Plasmacytosis 0 2 0 0 2 2 0 0 Congestion/erythrophagocytosis 0 0 0 0 0 0 0 3 BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOYOL/TELOMER ACRYLATE/' ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual 24nimal W.croscopic Findings 49 50 51' 52 53 54 55 56 m .m m m m m ni m necropsy status Liver P P P P P P P P Inflanrmatory cell foci Single cell necrosis 2 1 1 1 2 2 '2 1 1 1 0 0 0 1 0 0 Pancreas Apoptosis/single cell necrosis, 0 0 0 0 0 0 2 0 exocrine Kidneys Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 Hyaline cast (s) Adrenal Glands 0 0 0 0 0 1 0 1 Extracapsular nodule 0 0 0 0 0 0 0 X Spleen Hemopoiesis, primarily erythropoiesis 1 1 Hemosiderin pigment 0 1 Thymus Lymphoid atrophy - involution Lymphocytolysis, increased Mesenteric Lymph Node 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0. 2 . Congestion/erythrophagocytosis 0 0 0 1 0 '0 0 0 Mandibular Lymph Node Plasmacytosis 0 0 0 2 .o 0 2 0 Lymphoid hyperplasia 0 0 0 2 0 0 0 0 Congestion/erythrophagocytosis 0 0 0 2 0 0 0 0 BACK TO MAIN PATROLOGY REPORT ---- T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/' ./PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 27/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l Microscopic Findings Males, Dose group 4 57 50 59 61 62 63 m .m m m m m necropsy status I r r S s S Eyes Periorbital inflammation due to 3 blood sampling . ; .I . BACK TO MAIN PATIlOLOGY REPORT --c_- Page 281146 Test Article: TELOMER ALCOHOL/'I'ELOMER ACRYLATE/. :/PE'HS/PFOS NOTOX no. : 242933 ' Test System : Exploratory 28-Day Gavage Study in Rats Propath no.: 99010 Sponsor : 3~ Toxicology Services Date Z1.ApK. 1999 Individual A n i m a l mcroscopic Findings M e s , Dose group 5 65 66 67 . 68 69 70 71 72 m .m m m m m m m necropsy status Lungs P P P P P P P P Intra-alveolar blood, necropsy X artefact Vascular mineralisation, focal 1 _. Liver Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 2 1 2 2 2 2 2 2 1 0 1 0 1 1 1 1 3 3 3 3 3 2 2 3 Hepatocellular vacuolation Coagulative necrosis, focal Pancreas 2 0 1 0 0 0 0 1 0' 0 1 0 0 0 0 0 Apoptosis/single cell necrosis, 0 1 0 1 0 0 0 0 exocrine Inflaxmation, mixed granulocytic/lymphocytic 0 0 0 0 0 1 0 0 Kidneys Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1 1 1 1 0 0 0 0 0 0 0 1 1 1 0 0 Cystic tubules Testes 0 0 0 0 0 0 2 0 Seminiferous tubular atrophy Epididymides 0 4 0 0 0 0 0 0 Atrophy 3 Aspermia X BACK TO MAIN PATHOLOGY REPORT - - T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3~ Toxicology Services Page 29/146 NOTOX no. : 242933 Plropath no.: 99010 Date : 21.Apr.1999 Individual Animal Microscopia Findings m e s , Dose group 5 65 66 67 68 69 70 71 72 m .m m m m m m m necropsy status Adrenal Glands P P P P P P P P Extracapaular nodule 0 0 0 0 X 0 0 0 Spleen Hemopoiesis, primarily erythropoiesis 2 1 2 1 1 2 1 2 Hemosiderin pigment ' 0 1 0 2 0 0 1 2 Mandibular Lymph Node Plasmacytosis 0 3 2 0 0 0 2 2 Congestion/e rythr ophagocytosis 0 1 0 0 0 0 0 2 BACK TO MAIN PATHOLOGY REPORT --<I Page 30/146 Test Article: TELOMER =COHOL/TELOMER ACRYLATE/ /PFHS/ems Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services NOTOX no. : 242933 Piopath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l kdicrosaopic Findings W e s , Dose group 5 73 74 75 77 78 79 rn . m rn m m m necropsy status r r r S 3 3 Liver Inflaxmatory cell foci 3 2 2 2 2 2 Single c e l l necrosis 2 1 1 1 1 0 Midzonallcentrilobar hypertrophy, diffuse Hepatocellular vacuolation Testes 2 0 1 0 0 0 .o 0 0 0 0 1 Semhiferous. tubular atrophy 3 . :. BACK TO MAIN PATHOLOGY REPORT --\- Test Article: TELOMER ALCOHOL/TELMER ACRYLATE/ /PFHs/PFos Test System : `Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 31/146 NOTOX no. : 242933 Pfopath no.: 99010 Date : 21.Apr. 1999 Individual Animal Microscopic Findings Males, Dose group 6 0 1 02 8 3 - 84 85 86 a7 a0 m .m m m m m m m necropsy status Liver P P P P P P P P Inflannnatory cell foci Single cell necrosis MidzonaUcentrilobular hypertrophy, diffuse 2 2 2 2 1 2 2 2 1 0 1 1 0 1 1 1 2 3 3 3 3 2 3 3 Hepatocellular vacuolation Hematopoietic cell foci Pancreas 0 1 1 0 0 1 2 0 1 0 0 0 0 0 0 0 Apoptosis/single cell necrosis, 0 . exocrine 2 0 0 0 0 0 1 Kidneys Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1 1 0 1 1 1 1 0 0 0 2 1 0 0 0 0 Hyaline cast (s) Cystic tubules Testes 0 0 0 0 0 1 1 0 0 0 2 0 0 0 0 0 Spermatidic giant cell (s) Adrenal Glands 0 0 1 0 0 0 0 0 Vacuolation, multifocal in z. fasiculata 0 0 0 0 0 1 0 1 Atrophy, cortical diffuse 0 0 0 2 0 0 0 0 BACK TO MAIN PATHOLOGY REPORT ---- T e s t A r t i c l e : TELOMER ALCOUOL/kLOMER ACRYLATE/ / P M S /PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 32/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual. A n i m a l Microscopia Findings Males, Dose group 6 81 82 8 3 . 84 85 86 87 88 m .m m m m m m m necropsy status Spleen Hemopoiesis, primarily erythropoiesis Hemosiderin pigment ' 1 Thymus Lymphoid atrophy - involution Mandibular Lymph Node Plasmacytosis P P P P P P P P 1 1 1 1 1 2 1 1 1 1 1 0 0 0 0 1 0 0 0 0 0 2 0 1 3 0 0 2 0 0 0 0 BACK TO MAIN PATHOLOGY REPORT Test & t i d e : TELOMER ALCOHOL/TELOMER ACRYLATE/ .'PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual ?idma1Microscopic Findings Males, Dose g-roup 6 89 90 91 - 93 94 95 m .m rn m m m necropsy status r r r s s s Liver Inflamuatory cell foci 2 2 2 1 2 2 Single cell necrosis 1 1 0 1 0 1 Midzonal/centrilobular hypertrophy, diffuse 2 3 3 1 3 2 Hepatocellular vacuolation 1 2 2 0 1 0 BACK TO MAIN PATHOLOGY REPORT -:._- T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t S y s t e m : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services Page 34/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 I n d i v i d u a l Animal Microscopic F i n d i n g s Eemales, Dose group 1 necropsy status Liver 97 98 99 100 101 102 103 104 f .f f f f f f f P P P P P P P P Inflanunatory cell f o c i Single cell necrosis 2 1 2 1 2 2 2 1 1 0 1 1 1 1 1 1 Hepatocellular vacuolation Pancreas 0 0 0 0 0 0 0 1 Apoptosis/single cell necrosis, 0 1 1 0 0 0 0 0 exocrine Kidneys Tubular atrophy, cortical 1 0 0 1 0 1 0 1 Tubular mineralization 0 0' 0 0 1 0 0 0 Pelvic mineralization Spleen 0 1 0 0 0 0 0 0 Hemopoiesis, primarily erythropoiesis 1 1 1 1 0 1 1 1 Hemosiderin pigment 3 2 2 2 3 3 2 2 Thymus ~* Lymphoid atrophy - involution 0 0 0 0 0 0 1 0 M a n d i b u l a r Lymph Node Lymphoid hyperplasia 0 0 0 0 2 0' 0 0 BACK TO MAIN PATHOLOGY REPORT --c- Test ActiCle: TELOMER ALCOHOL/TELOMER ACRYLATE/ !PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 35/146 NOTOX no. : 242933 Piopath no.: 99010 Date : 21.Apr.1999 Individual Animal Microscopio Findings Females, Dose group 1 105 106 107 109 110 111 f .f f f f f necropsy status r r r S S s Liver Inflammatory cell foci 2 2 2 2 2 1 Single cell necrosis 1 1 0 1 0 0 Hepatocellular vacuolation . 0 2 0 0 0 0 Skin Inflanunation, exudative 2 BACK TO MAIN PATHOLOGY REPORT ----a Test Article: TELOMER ALCOHOL/TELCPZEARCRYLRTW .IPFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 36/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Aninul Xicroscopic Findings Females, Dose grorrp 2 113 114 115 116 117 118 119 120 f .f f .f f f f f necropsy status Liver P P P P P P P P Inflarmnatory cell foci 2 2 1 1 1 1 1 2 Single cell necrosis 1 1 0 1 0 1 1 1 Kidneys Tubular atrophy, cortical 0 0 0 0 0 0 1 0 Pelvic dilation 0 0 0 0 X 0 0 0 Adrenal Glands Extracapsular nodule 0 0 0 0 X 0 0 0 Spleen Hemopoiesis, primarily erythropoiesis 1 1 1 1 1 1 1 1 Hemosiderin pigment Thymus Lymphoid atrophy - involution Lymphocytolysis, increased 2 2 2 2 2 2 3 2 0 0 0 0 0 .1 0 0 0 0 0 0 0 0 1 0 Mandibular Lymph Node Plasmacytosis 2 2 0 0 0 3 0 0 Lymphoid hyperplasia 2 0 0 0 0 0 0 0 BACK TO MAIN PATIIOLOGY REPORT - -6 Page 37/146 Test Article : TELOMER ALCOHOL/TELCMERACRYLATE/ ../ems/pFos NOFOX no. : Test System : Exploratory 28-Day Gavage Study in Rats ' Propath no.: Sponsor : 3M Toxicology Services Date : 242933 99010 21 .Apr. 1999 Individual h i m a l mcrosaopio Findings Females, Dose group 3 necropsy status Liver 129 130 131 132 133 134 135 136 f .f f f f f f f 0 P P P P P P P Inflanrmatory cell foci 2 2 1 1 2 1 1 2 Single cell necrosis 1 1 1 0 2 0 0 1 Hepatocellular vacuolation Pancreas 0 1 0 0 0 0 0 0 Exocrine atrophy, focal Kidneys 0 0 0 1 0 .o 0 0 T u b u l k c atrophy, cortical 0 1 0 0 0 1 0 0 Interstitial inflammation, lymphocytic 0 0 0 0 0 1 0 1 Adrenal G l a n d s Extracapsular nodule Spleen 0 0 0 0 0 0 0 X Hemopoiesis, primarily erythropoiesis 2 1 1 1 2 1 1 1 Hemosiderin pigment 1 2 2 1 2 2 2 3 . . Thymu Lymphoid atrophy - involution 0 1 0 0 1 0 0 1 Lymphocytolysis, increased 0 0 0 0 0 1 0 0 Mandibular Lymph Node Plasmacytosis 0 0 0 0 0 0 2 2 Lymphoid hyperplasia 0 0 0 0 0 0 2 2 ~ BACK TO MAIN PATHOLOGY REPORT Page 38/146 Test Article: TELCMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services NOTOX no. : 242933 Pfopath no.: 99010 Date : 21.Apr.1999 Individual AnimaJ. Microscopic Findings E d e s , Dose group 4 necropsy status Liver Inflammatory cell foci Single cell necrosis Hematopoietic cell foci .. Kidneys T u b u l a r atrophy, c o r t i c a l Interstitial inflammation, lymphocytic -1 2 1 2 2 2 2 1 0 1 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 Cystic tubules Uterw 0 0 2 0 0 0 0 0 Cyst(s) X Spleen Hemopoiesis, primarily erythropoiesis 1 1 0 1 1 0 0 1 Hemosiderin pigment 2 2 2 2 2 3 2 2' Thymus Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node 0 0 0 0 0 0 0 .1 0 0 0 1 0 0 0 0 Plasmacytosis 0 0 0 0 0 2 0 0 Lymphoid hyperplasia 2 0 2 0 0 2 0 0 BACK TO MAIN PATHOLOGY REPORT --c- Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 39/146 NOTOX no. : 242933 Prepath no.': 99010 Date : 21.Apr.1999 Individual Animal Microscopic Findings Females, Dose g z o q 5 161 162 163 164 165 166 167 168 f .f f f f f f f necropsy status Liver P P P P P P P P Inflammatory cell foci 2- 2 3 2 1 1 1 1 Single cell necrosis 0 1 1 0 1 0 1 1 Hepatocellular vacuolation 0 0 0 0 0 0 0 1 Pancreas Apoptosis/single cell necrosis, 0 0 0 0 1 0 0 0 exocrine Kidneys Cystic tubules 0 0 0 0 0 0 0 1 Tubular epithelial hypertrophy, 1 0 0 0 0 0 0 0 foca1 Adrenal Glands Extracapsular nodule 0 0 0 0 0 X 0 0 Spleen Hemopoiesis, prharily erythropoiesis 1 1 2 1 1 1 1 1 ., Hemosiderin pigment Thymus Lymphoid atrophy - involution Lymphocytolysis, increased 2 2 2 2 2 2 2 3 0 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 Mandibular Lymph Node Plasmacytosis 0 0 2 0 0 0 0 0 Lymphoid hyperplasia 0 0 0 0 0 2 0 0 BACK TO MAIN PATHOLOGY REPORT --< - T e s t Article : TELOMER ALCOHOL/TELOMER ACRYLATE/ i/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 40/146 NOTOX no. : Prbpath no.: Date : 242933 99010 21.Apr.1999 Individual Animal Microscopic Findings Femaleas, Dose group 6 necropsy status Liver 177 178 179' 1 8 0 1 8 1 182 183 1 8 4 f .f f f f f f e P P P P P PPP Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 2 1 2 2 1 1 1 1 1 0 1 0 1 0 1 0 2 2 3 1 2 3 3 2 Kidneys Tubular atrophy, cortical 1 1 0 0 1 1 0 1 Interstitial inflammation, lymphocytic 0 1 0'1 0 0 1 0 Adrenal Glands Extracapsular nodule Spleen X 0 0 0 0 0 0 0 Hemopoiesis, primarily erythropoiesis 1 0 0 1 1 1 0 1 Hemosiderin pigment 2 1 3 2 3 2 2 2 Thymus - Lymphoid atrophy involution ,. Mandibular Lymph Node ' 1 0 0 0 0 0 0 0 Plasmacytosis 0 0 0 0 0 2 2 0 BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 P?opath no.: 99010' Date : 21.Apr.1999 Individual Animal Wcroscopic Findings necropsy status Liver Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diifuse 185 186 187 189 190 191 f .f f f f f r r r s s s 1 1 2 2 1 1 ., 0 0 1 1 1 0 0 1 0 0 0 1 BACK TO MAIN PATHOLOGY REPORT Page 42/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Aaimdl D a t a List &!ales ANIMAL NUMBER SEXFINAL M/F STATE TEST DAYS FIRST DAY . DOSE GROUP 1 Imp 2mP 3mP 4mP 5mP 6mP 7mP 8mP 9mr 10 m I: 11 m r 13 m s 14 m s 15 m s DOSE GROUP 2 17 m p 18 m p 19 m p 20 m p 21 m p 22 m p 23 m p 24 m p 25 m r 26 m r 27 m r 29 m s 30 m s 31 m s DOSE GROUP 3 33 m p 34 m p 35 m p 36 m p 37 m p 38 m p 39 m p 40 m p 41 m r 42 m r 43 m r 45 rn s 46 m s 47 m s 28 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/90 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 LAST DAY 02/11/98 02/11/98 02/11/98 02/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 02/11/98 02/11/98 02/11/98 02/11/98 03/11/90 03/11/98 03/11/98 03/11/90 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 02/11/98 02/11/98 02/11/98 02/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/90 03/11/90 03/11/98 WE NECROPSY 03/11/98 03/11/98 03/11/98 03/11/98 04/11/98 04/11/98 04/11/98 04/11/98 18/11/98 18/11/98 18/11/98 02/12/98 02/12/98 02/12/98 03/11/98 03/11/98 03/11/98 03/11/98 04/11/98 04/11/98 04/11/98 04/11/98 18/11/98 18/11/98 18/11/98 02/12/98 02/12/98 02/12/98 03/11/98 03/11/98 03/11/98 03/11/98 04/11/98 04/11/98 04/11/98 04/11/98 18/11/98 18/11/98 18/11/98 02/12/98 02/12/98 02/12/98 BACK TO MAIN PATIlOLOGY REFORT - C L Test Article!: TELCMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 43/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Animal D a t a List bales ANIMAL SEX FINAI, TEST FIRST NUMBER M/FSTATE DAYS DAY . DOSE GROUP 4 49 m P 50 m P 51 m P 52 m P 53 m P 54 m P 55 m P 56 m P 57 m r 58 m r 59 m r 61 m s 62 m s 63 m s DOSE GROUP 5 65 m P 66 m P 67 m P 68 m P 69 m P 70 m P 71 m P 72 m P 73 m r 74 m r 75 m r 77 m s 78 m s 79 m s DOSE GROUP 6 81 m P 02 m P 83 m P 84 m P 85 m P 86 m P 87 m P 88 m P 89 m r 90 m r 91 m r 93 m s 94 m s 95 m s 28 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 28 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/90 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 20 06/10/90 28 06/10/98. 20 06/10/98 28 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 29 06/10/98 LAST DAY 02/11/98 02/11/98 02/11/98 02/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 02/11/98 02/11/98 02/11/98 02/11/98 03/11/98 03/11/98 03/11/98 03/11/90 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 02/11/98 02/11/98 02/11/98 02/11/90 03/11/98 03/11/98 03/11/98 03/11/98 03/11/98 03/11/90 03/11/98 03/11/98 03/11/98 03/11/98 DATE NECROPSY 03/11/98 03/11/90 03/11/98 03/11/98 04/11/98 04/11/98 04/11/98 04/11/98 18/ 11/98 18/11/98 18/11/98 02/12/98 02/12/98 02/12/98 03/11/98 03/11/98 03/11/98 03/11/98 04/11/98 04/11/98 04/11/98 o4/ii/ga 18/11/90 18/11/98 18/11/98 02/12/98 02/12/90 02/12/98 03/11/98 03/11/90 03/11/98 03/11/90 04/11/98 04/11/98 04/11/98 04/11/98 ia/ii/ga 18/11/98 16/11/90 02/12/90 02/12/98 02/12/90 BACK TO MAIN -7%- PATHOLOGY REPORT ./PFHS/PFOS T e s t Article: TELmER aconoL/TEtc+m ACRYLATE/ T e s t System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3 M Toxicology Services Page 44/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 A n i m a l D a t a List Fdes ANIMAL NUMBER SEX FINAL M/F STATE TEST DAYS FIRST DAY . DOSE GROUP 1 97 f P 98 f P 99 f P 100 f P 101 f P 102 f P 103 f P 104 f P 105 f r 106 f r 107 f r 109 f s 110 f s 111 f s DOSE GROUP 2 113 f P 114 f P 115 f P 116 f P 117 f P 118 f P 119 f P 120 f P 121 f r 122 f r 123 f r 125 f s 126 f s 127 f s DOSE GROUP 3 129 i o 130 f P 131 f P 132 f P 133 f P 134 . f P 135 f P 136 f P 137 f r 138 f r 139 f r 1 41 f s 142 f s 143 f s 28 08/10/98 28 08/10/98 28 08/lQ/98 28 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/90 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 28 08/10/98 28 08/10/98 28 08/10/98 28 08/10/98 29 08/10/98 29 08/10/98 29' 08/10/90 29 08/10/98 29 00/10/98 29 08/10/98 29 08/10/98 29 Q8/10/98 29 08/10/98 29 08/10/98 28 08/10/98 28 08/10/98 28 08/10/98 28 08/10/98 29 08/10/98 29 08/10/98 29 08710/98 29 00/10/98 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 29 08/10/98 LAST DAY 04/11/98 04/11/98 04/11/98 04/11/98 05/11/98 05/11/98 05/11/98 05/11/98 05/11/98 05/11/90 05/11/98 05/11/98 05/11/98 05/11/98 04/11/98 04/11/98 04/11/98 04/11/98 05/11/98 05/11/98 05/11/98 05/11/98 05/11/90 05/11/98 05/11/98 05/11/98 05/11/98 05/11/98 04/11/98 04/11/98 04/11/98 04/11/98 05/11/90 05/11/90 05/11/98 05/11/98 05/11/98 05/11/98 05/11/98 05/11/98 05/11/98 05/11/90 DATE NECROPSY 05/11/98 05/11/98 05/11/98 05/11/98 06/11/98 06/11/98 06/11/98 06/11/98 20/11/98 20/11/98 20/11/98 04/12/98 04/12/98 04/12/98 05/11/98 05/11/98 05/11/98 05/11/98 06/11/98 06/11/98 06/11/98 06/11/98 20/11/98 20/11/98 20/11/98 04/12/98 04/12/98 04/12/98 05/11/98 05/11/98 05/11/98 05/11/90 06/11/98 06/11/98 06/11/98 0 6/11/98 20/11/98 20/11/98 20/11/98 04/12/98 04/12/98 04/12/90 BACK TO MAIN T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t S y s t e m : E x p l o r a t o r y 28-Day Gavage S t u d y i n Rats Sponsor : 3~ T o x i c o l o g y S e r v i c e s NOTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Data List Females ANIMRL NUMBER SEX FINAL M/F STATE TEST DAYS FIRST DAY LAST DAY OATE NECROPSY DOSE GROW? 4 145 f p 20 08/10/98 04/11/98 05/11/90 1 46 f p 147 f p 28 00/10/98 28 08/10/90 04/11/98 04/ 11/98 05/11/98 05/11/98 148 f p 28 08/10/98 04/11/ 98 05/11/98 1 49 f p 29 08/10/90 05/11/98 06/11/98 150 f p 29 08/10/90 05/11/98 06/11/90 I 151 f p 29 00/10/90 05/11/98 06/11/90 152 f p 29 00/10/90 05/11/90 06/11/90 153 f r 154 f r . 155 f r 157 f s 29 00/10/98 05/11/98 29 08/ 10/ 98 05/11/98 29 00/10/90 05/11/90 29 08/10/98 05/11/90 20/11/90 20/11/98 20/11/98 04/12/98 150 f s 29 08/10/90 05/11/90 04/12/90 159 f s 29 00/10/98 05/11/98 04/12/98 DOSE GROUP 5 161 f p 20 00/10/90 04/11/98 05/11/98 162 f p 20 oe/io/ga 04/11/90 05/11/90 163 f p 28 08/10/98 04/11/98 05/11/98 164 f p 28 08/10/90 04/11/98 05/11/98 165 f p 29 00/10/98 05/11/90 06/11/98 166 f p 29 08/10/98 05/11/98 06/11/98 167 f p 29 08/10/98 05/11/98 06/11/98 168 f p 29 08/10/98 05/11/98 06/11/98 169 f r 29 08/10/98 05/11/98 20/11/98 170 f r 29 00/10/98 05/11/98 20/11/98 171 f r 29 00/10/98 05/11/98 20/11/98 173 f s 29 00/10/98 05/11/98 04/12/90 174 f s 29 00/10/98 05/11/90 04/12/98 175 f s 29 08/10/90 05/11/98 04/12/98 DOSE GROUP 6 177 f p 28 08/10/90 04/11/98 05/11/98 '. . 178 f p 28 08/10/98 04/11/98 05/11/98 179 f p 28 08/10/98 04/11/98 05/11/98 100 f p 28 00/10/90 04/11/98 05/11/98 101 f p 29 08/10/90 05/11/98 06/11/98 182 f p 29 00/10/98 05/11/98 06/11/98 183 f p 29 08/10/90 05/11/98 06/11 f98 184 f p 29 08/10/98 05/11/98 06/11/98 185 f r 29 08/10/90 05/11/98 20/11/98 186 f r 29 08/10/98 05/11/90 20/11/98 187 f r 29 08/10/98 05/11/98 20/11/98 109 f s 29 08/10/98 05/11/98 . 04/12 /98 190 i s 29 08/10/98 05/I1/90 04/12 /90 191 f s 29 00/10/90 05/11/90 04/12/98 BACK TO MAIN PATgOLOGY REPORT --._e Test Article: TELCHER ALcoHoL/TELCMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Kats Sponsor : 3~ Toxicology Services Page 46/146 NOTOX no. : 242933 P2opath no.: 99010 Date : 21.Apr. 1999 Individual Animal Data RecordS Animal No: SEX: DOSE GROUP: 1 Male Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 M h a l No: 2 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : tenuinal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/31/98 No findings noted Inflammatory cell foci (slight) Hematopoietic cell foci (minimal) Hemopoiesis, primarily erythropoiesis (slight) Plasmacytosis (slight) Plasplacytosis (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal GlanW2, Thymus, Mesenteric Lymph Node. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned tenuinal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted BACK TO MAIN PATHOLOGY =PORT --\1 Test Article: TELCMER ALCOHOL/TELOMER ACRYLATE/ IPFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 47/146 NOTOX no. : 242933 Piopath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Rccorda Animal No: SEX: DOSE GROUP: 2 Male Vehicle Control MICROSCOPIC FINDINGS Liver Tested1 Testes/;! Spleen Mandibular Lymph Node/ 1 Animal No: 3 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen Mandibular Lymph Node/ 1 Inflammatory cell foci (slight) Single cell necrosis (minimal) Seminiferous tubular atrophy (minimal) Spermatidic giant cell(s) (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Adrenal Gland/l, Adrenal Gland/P, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal. sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hematopoietic cell foci ( m i n i m a l1 Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) Lymphoid hyperplasia (slight) BACK TO MAIN PATHOLOGY REPORT Test Article: TEL-R ALCOHOL/TELOMER ACRYLATE/ tPFHS/PFOs Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 48/146 NOTOX no. : 242933 , Piopath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records A n i m a l No: SEX: DOSE GROUP: 3 Male Vehicle Control MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 2 Animal No: 4 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Wdney/l Spleen Mandibular Lymph Node/ 1 Plasmacytosis (slight) No abnormalities found in: Xidney/l, Kidney/2, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosi.derinpigment (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/2, Testes/l, 'Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT --cl T e s t Article: TELOMER XCOHOL/TELOMER ACRYLATE/ ./PFHs/PFos T e s t System : E x p l o r a t o r y 20-Day Gavage Study i n R a t s Sponsor : 3M Toxicology S e r v i c e s Page 491146 NOTOX no. : Prbpath no.: Date : 242933 99010 21.Apr.1999 Individual Animal Data Records Animal. No: SEX: DOSE GROUP: 5 Male Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas ICidney/l Spleen Animal No: 6 MACROSCOPIC FINDINGS days of treatment : 29 s a c r i f i c e group : terminal sacrifice necropsy status : planned tenufnal. date of s t a r t o treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy :. 04/11/90 No findings noted Inflammatory c e l l foci (minimdl1 Single c e l l necrosis (minimal) Apoptosis/single c e l l necrosis, exocrine (slight) I n t e r s t i t i a l inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimdl1 Hemosiderin pigment (slight) No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/P, T h m s , Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. days of treatment : 29 s a c r i f i c e group : tenninal sacrifice necropsy status : planned telminal date of s t a r t of treatment: 06/10/98 date of end o f treatment: 03/11/90 date of necropsy : 04/11/90 No findings noted BACK TO MAIN PATHOLOGY REPORT --..- Test Article: TELOMER ALCOHOL/TELOMER ACRYLRTE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 50/146 NOTOX no. : Piopath no.: 242933 . 99010 Date : 21.Apr.1999 Individual Animal D a t a Records A n h a l No: SEX: DOSE GROUP: 6 Male Vehicle Control MICROSCOPIC FINDINGS Liver Kidney/l Kidney/2 Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 7 MACROSCOPIC FINDINGS MICROSCOPIC FIhiDINGS Liver . Kidney/l Inflammatory call foci (minimal) Single cell necrosis (minimal) m a r atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Tubular atrophy, cortical (minimal1 Hemopoiesis, primarily erythropoiesis ( m i n i m a l1 Hemosiderin pigment (slight) Plasmacytosis (moderate) Plasmacytosis (moderate) No abnormalities found in: Pancreas, Testedl, Testas/Z, Adrenal Gland& Adrenal Gland/2, Thymus, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned t e r m i n a l date of start of trea-t: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (slight) Hepatocellular vacuolation (minimal) Hematopoietic cell foci (minimal) Hyaline cast(s) (minimal) BACK TO MAIN PATHOLOGY REPORT --._- Test Article: TELOMER ALCOHOL/TELOMEX ACRYLATE/ 'PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 51/146 NOTOX no. : 242933 Pr'opath no.: 99010 Date : 21 .Apr. 1999 Individual A n b d , Data Record8 b h a l No: 7 SEX: Male DOSE GROUP: . Vehicle Control MICROSCOPIC FINDINGS (continued) Spleen Animal No: 8 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Kidney/Z Spleen Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal1 No abnormalities found in: Pancreas, I(idney/2, Testes/1, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Tubular atrophy, cortical (mMmdl) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis ( r n h h d . 1 No abnormalities found in: Pancreas, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular L y q h Node/2. BACK TO MAIN -TL.I PATHOLOGY REPORT T e s t A r t i c l e : TELOMER ?UCOHOL/TELCNER ACRYLATE/ /P!?HS/PmS Test System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 314 Toxicology S e r v i c e s Page 52/146 NOFOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 9 Male Vehicle Control MACROSCOPIC FINDINGS Skin HICROSCOPIC FINDINGS Liver Animal No: 10 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment : 29 s a c r i f i c e group : recovery group necropsy s t a t u s : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 chest region: Alopecia No abnormalities were found in any of t h e other t i s s u e s examined Inflammatory c e l l foci ( s l i g h t ) No abnormalities found in: Pancreas. days of treatment : 29 s a c r i f i c e group : recovery group necropsy status : planned recovery date of s t a r t of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted Inflammatory cell foci (moderate) Single c e l l necrosis (slight) No abnormalities found in: Pancreas. BACK TO MAIN ----..e. PA!PHOLOGY REPORT Test Article: TELOMER ALcOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 314 Toxicology Services Page 53/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual IInimal Data Records Animal No: SEX: DOSE GROUP: 11 Male Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 13 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) No abnormalities found in: Pancreas. days of treatment : 29 sacrifice group : recovery group 2 nbcropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) No abnormalities found in: Pancreas. BACK TO MAIN PATHOLOGY REPORT --_- Test Article: TELOMER ALCOHOLITELOMERACRYLATE/ /ems/eFos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 54/146 NOTOX no. : 242933 Pr'opath no.: 99010 Date : 21.Apr.1999 Indi-ddual Animal Data Records A n i m a l No: SEX: DOSE GROUP: 14 Male Vehicle control MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/90 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minimal) No abnormalities found in: Pancreas. h i m d l No: 15 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery grovp 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 8 ...,. . MICROSCOPIC FINDINGS No findings noted Liver Inflammatory cell foci (slight) Pancreas Apoptosis/single cell necrosis, exocrine (minimal) BACK TO MAIN PATHOLOGY =PORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ :/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 551146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Ankadl. Data Records Animal No: SEX: DOSE GROUP: 97 Female Vehicle Control MACROSCOPIC FIND1N.a MICROSCOPIC FINDINGS Liver Ki.dney/l Spleen A n b a l No: 98 MACROSCOPIC FINDINGS Ovaries days of' treatment : 28 sacrifice group : termind sacrifice necropsy statu3 : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted 1nfJ.ammatory cell f o d (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start o f treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 left side Watery cyst No abnormalities were found in any of the other tissues examined BACK TO MAIN PATIfOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 56/146 NO?OX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual Animal D a t a Reaorda Animal No: SEX: DOSE GROUP: 98 Femdle Vehicle Control MICROSCOPIC FINDINGS Liver . Pancreas Kidney/l ovarY/l Spleen AnimaJ. No: 99 MACROSCOPIC FINDINGS . I MICROSCOPIC FINDINGS Liver Pancreas Spleen Inflammatory cell foci (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Pelvic mineralization (minimal) No micro correlate Hemopoiesis, primarily erythropoiesis (minimdl1 Hemosiderin pigment (slight) No abnormalities found in: Kidney/2, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibula Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status .: planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/96 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) woptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Kidney/l, Kidney/2, m r y / l , Ovary/Z, Adrenal Glax@/l, Adrenal Gl=d/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. BACK TO MAIN RATHOLOGY REPORT ----.a Test Article: T E L W R ALCOHOL/TELOMER ACRYLATE/ 3/PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 57/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records Animal No: SEX: DOSE GROUP: 100 Female Vehicle Control MACROSCOPIC FINDINGS .' . MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animal No: 101 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/90 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical ( z d n h a l ) Hemopoiesis, primarily erythropoiesis (minimal) . ' Hemosiderin pigment (slight) NO abnormalities found in: Pancreas, Kidney/2, Ovary/l, Gvary/Z, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lynqh. Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned tenainal date o f start of treatment: 08/10/90 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 , No findings noted BACK TO MAIN PATHOLOGY REPORT - - . 1 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFoS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 58/146 NOTOX no. : 242933 PrGpath no.: 99010 Date : 21.Apr.1999 Individual Aninuf Data Records Animal No: SEX: DOSE GROUP: 101 Female Vehicle Control MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minimal) Ktdney/l Tubular mineralization (minimal) Spleen Hemosiderin pigment (moderate) Mandibular Lymph Node/ 1 Lpphoid hyperplasia (slight) NO abnormalities found in: Pancreas, Kidney/2, Ovary/l, Ovary/2, Adrenal ~land/l,Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. Animal No: 102 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal. date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted MICROSCOPIC E'INDINGS ._. ... Liver Inflammatory cell foci (slight) single cell necrosis (minimal) Mdney/l Spleen Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis ( m i n u ) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/2, m r y / l , Ovary/2, Adrenal Gland/l, Adrend GlanU2, Thymus, Mesenteric Lymph Node, Mandibular L m h Node& Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Test Article: TELCMER AS,COHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 59/146 NOTOX no. : 242933 PrGpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 103 Female Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 104 MACROSCOPIC FINDINGS .. ,' .. . . days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned termindL date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimdl) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal.) No abnormalities found in: Pancreas, Kidney& Kidney/2, Ovary/ll Ovary/2, Adrenal Gland/l, Adrenal. Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular L m h Node/2. days of treatment : 29 sacrifice group : t e d n a l sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN ---...- PATIfOLOOY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 314 Toxicology Services Page 60/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual A n h d D a t a Records An- No: SEX: DOSE GROUP: 104 Female Vehicle Control MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animal No: 105 MACROSCOPIC FINDINGS Skin MICROSCOPIC FINDINGS Liver Skin Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal.) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/Z, Ovary/l, Ovary/2, Adrenal. Gland/l, Adrenal Gland/Z, Thymus, Mesenteric Lynph Node, Mandibular Lymph Ncde/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 abdominal region: scab formation No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Inflammation, exudative (slight) (correlates to GROSS finding) BACK TO MAIN --c PATHOLOGY REPORT Test Article: TELCMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/pFos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 61/146 NOTOX no. : 242933 Pripath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l Data Records Animal No: SEX: DOSE GROUP: 106 Female Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver AnimdL No: 107 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 109 MACROSCOPIC FINDINGS days of treatment: : 29 sacrifice group : recovely group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (slight) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory cell foci (slight) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 BACK TO MAIN PATtIOMGY REPORT -- --I T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ JPFHWPFOS T e s t System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services Page 62/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records A d m d . No: SEX: DOSE GROUP: 109 Female vehicle Control MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Animal No: 110 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 111 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver No findings noted Inflammatory c e l l foci ( s l i g h t ) Single c e l l necrosis (minimal) days of treatment : 29 s a c r i f i c e group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted Inflammatory cell foci ( s l i g h t ) days of treatment : 29 s a c r i f i c e group : recovery group 2 necropsy status : planned recovery 2 date of s t a t of treatment: 00/10/90 date of end of treatment: 05/11/98 date of necropsy : 04/12/90 No findings noted Inflammatory cell' foci (minimal) BACK TO MAIN PATHOLOGY REPORT -7c1 Test Axticle: T E ~ M E RALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Explozatory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 63/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr. 1999 Individual Animal Data Records Animal No: SEX : DOSE GROUP: 17 Male Telomer Xlcohol MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : temindl sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (minimal) Single cell necrosis (minimal) Pancreas Apoptosis/single cell necrosis, exocrine (slight) Kidney/ 1 Tubular atrophy, cortical (slight) ICidney/2 Tubular atrophy, cortical ( m i n i m a l ) Spleen Hemopoiesis, primarily erythropoiesis (minimal) Mandibular Lymph Node/ 1 Plasmacytosis (slight) 9. ,', Animal No: 18 No abnormalities found in: Testes& Testes/P, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice . necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 . date of necropsy : 03/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT --c- Test Article: TELWER ALCOHOLITELOMERACRYLATE/ ,WHSIPFOS * Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 64/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 18 Male Telcaner Alcohol MICROSCOPIC FINDINGS Liver Pancreas Kidney/l KidneyR Spleen Mandibular Lymph Node/ 1 A n i m a l No: 19 MACROSCOPIC FINDINGS MICROSCOPIC FINDING Liver Spleen Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical ( m i n i m a l ) Pelvic mineralization (minimal) Tubular mineralization ( m i n i m a l ) Hemopoiesis, primarily erythropoiesis (a) Hemosiderin pigment (minimdl) Plasmacytosis (moderate) No abnormalities found in: Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 . sacriiice group : tenninal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatmanti 02/11/98 date of necropsy : 03/11/98 No findings noted Inflarmnatory cell foci (minimal) MidzonaUcentrilobular hypertrophy, diffuse (minimal) Hemapoiesis, primarily erythropoiesis 1-( Hemosiderin pigment (minimal) BACK TO MAIN PATIIOLOGY REPORT ---.a Test Article: TEL~MERALCOHOLITELOMER ACRYLATE/ /pms/eFos Test S y s t e m : Exploratory 28-Day Gavage Study i'n Rats Sponsor : 3M Toxicology Services Page 65/146 N ~ T O X no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Animdl No: SEX: DOSE GROUP : 19 Male Telomer .Alcohol MICROSCOPIC FINDINGS (continued) Mesenteric Lymph Node Mandibular Lymph Node/ 1 Animdl No: 20 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Kidney/l Spleen Congestion/erythsophagocytosis (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/Z, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/Z, Thymus, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal. sacrifice necropsy status : planned termCnal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobd.ar hypertrophy, diffuse (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal1 Hemopoiesis, primarily erythropoiesis (-1) Hemosiderin pigment (slight) No abnormalities found in: Kidney/2, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/Z, T h W S , Mesenteric L y q h Node, Mandibular L p p h Node/18 M a n d i b u l a x Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT --c- Page 66/146 T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFos T e s t System : E x p l o r a t o r y 28-Day Gavage Study in Rats' Sponsor : 3r4 Toxicology S e r v i c e s NOTOX no. : Piopath no.: Date : 242933 99010 21.Apr.1999 Individual A n i m a l Data Pacords Ani.mal No: SEX: DOSE GROUP: 21 Male Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver ICidney/l Kidney/2 Spleen Mandibular Lymph Node/ 1 .. A n i m a l No: 22 MACROSCOPIC FINDINGS days of treatment : 29 s a c r i f i c e group : terminal sacrifice necropsy status : planned terminal date of s t a r t of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflamaatory c e l l foci (slight) Single c e l l necrosis (minimal) Midzonal /centrilobular hypertrophy, (minimal) diffuse I n t e r s t i t i a l inflammation, Lymphocytic ( m i h l ) Hyaline cast(s) (minimal) Tubular atrophy, c o r t i c a l (minimal) I n t e r s t i t i a l inflammation, lymphocytic ( ~ n l m a l ) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Testes/l, Testes/l, Adrenal Gland/l, Adrenal Gland/P, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/Z. days of treatment : 29 s a c r i f i c e group : terminal s a c r i f i c e necropsy status : planned t e d n a l date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date o f necropsy : 04/11/98 BACK TO MAIN -5c- T e s t Article: TELWER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 67/146 NOFOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 22 Male Telamer Alcohol MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Pancreas Ic. l.dney/l fidney/2 Spleen An- No: 23 MACROSCOPIC FINDINGS No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Apoptosis/single cell necrosis, exocrine (slight) Tubular atrophy, cortical (minimdl1 Tubular atrophy, cortical (mMmal1 Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Testes& Tested?; Adrenal Gland/l, Adrenal Gland/2, T h p s , Mesenteric Lymph Node, Mandibular L p p h Node& Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned t e n n i n a l date of start of treatment: 06/10/90 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted BACK TO MAIN RA!CIOMGY REPORT --._- Test Article: TELOMER ALCOHOL/TELOMERACRYLATE/ nnts/ems Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 68/146 NOTOX no. : 242933 Prbpath n0.i 99010 Date : 21.Apr. 1999 Individual Animal Data Records 3mixaal No: SEX: DOSE GROUP: 23 Male Telamer Ju,cohol MICROSCOPIC FINDINGS Liver Pancreas Spleen Mandibular L=-- Node 1 Animal No: 24 MACROSCOPIC FINDINGS .. MICROSCOPIC FINDINGS Liver Pancreas Spleen Inflammatory cell foci (slight) ' Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (minimdl) Hemosiderin pigment (minimal) Plasmacytosis (slight) No abnormalities found in: Kidney/l, Kidney/Z, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal. date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 NO findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzondl/centrilobular hypertrophy, diffuse (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Exocrine atrophy, focal (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (ndnhal) BACK TO MAIN PATHOLOOP REPORT Test Article: TELmER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 69/146 NOTOX no. : 242933 Prbpath no.: ' 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 24 . Male Telomer Alcohol MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 1 Plasmacytosis (slight) No abnormalities found in: Kidney/l, Kidney/Z, Testes/l, Testes/?, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. Animal No: 25 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy : 18/11/98 No findings noted MICROSCOPIC FINDINGS Liver \.. Animal No: 26 Inflammatory cell foci (slight) Single cell necrosis (minimal) Hematopoietic cell foci (rdnfmdL) NO abnormalities found in: Pancreas. MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment:,03/11/98 date of necropsy : 18/11/98 No findings noted BACK TO MAIN - *._ PATHOLOGY REPORT Test Article : TELMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 70/146 NOTOX no. : 242933 Prbpath no.: 99010' Date : 21.Apr.1999 Individual Animal. D a t a Records Animdl No: SEX: DOSE GROUP: 26 Male Telmer Alcohol MICROSCOPIC FINDINGS Liver Inflamrmatory cell foci (moderate) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (miniIIIa1) . I. No abnormalitiea found in: Pancreas. Animal No: 27 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of ataxt of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 Testes Reduced in size ' ~ A c C I D No abnormalities were found in any of the other tissues examined MICROSCOPIC FINDINGS Liver Pancreas Inflammatory cell foci (moderate) Single cell necrosis (minimal) Exocrine atrophy, focal (mMmal) Testea/l Seminiferous tubular atrophy (severe) (correlatesto GROSS f h d h g ) Tested2 Seminiferous tubular atrophy (severe) (correlates to GROSS finding) BACK TO MAIN PATHOLOGP REPORT --.._- Page 71/146 Test Article: TELWR ALCOHOLITSLOMER ACRYLATE/ /PFHS/PEDS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21 .Apr. 1999 In&vidual Animal D a t a Records &l No: SEX: DOSE GROUP: 29 Male Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS LiWE Animdl No: 30 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment : 29 sacrifice group : recovary group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Midional/centrilobular hypertrophy, diffuse (minimal) No abnormalities found in: Pancreas. days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Coagulative necrosis, focal (minimal) No abnormalities found in: Pancreas. BACK TO MAIN --5_- PATHOLOGY REPORT Test Article: T E ~ M E RmCOHOL/TELOMER ACRYLATE/ '. IPFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 72/146 NOTOX no. : PGopath no.: 242933 99010 Date : 21.Apr.1999 Individual Animal Data Records A n h a l No: SEX : DOSE GROUP: 31 Male Telamer Alcohol MACROSCOPIC FINDINGS . ... days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minimal) No abnormalities found in: Pancreas. Animdl No: SEX: 113 Female MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minimal) Spleen Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Mandibular Lymph Node/ 1 Plasmacytosis (slight) Lymphoid hyperplasia (slight) BACK TO MAIN Test Article: TELOMER ALCOHOL/TELOMER ACRYLRTEI /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 731146 NOTOX no. : 242933 P'rbpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX.: DOSE GROUP: 113 Female Telmer Alcohol MICROSCOPIC FINDINGS (continued) No abnormalities fwnd in: Pancreas, Kidney/l, Kidney/2, Ovaxy/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node12. Animal No: 114 MACROSCOPIC FINDINGS days of treatment : 28 sacxifice group : terminal sacrifice nectopsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/90 date of necropsy : 05/11/98 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minimal) Spleen Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Mandibular Lymph Node/ Plasmacytosis (slight) !. . NO abnormalities found in: Pancreas, Kidney/l, adneY/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT --c- T e s t A r t i c l e : TEMMER JUCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 74/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Animdl D a t a Records Animal No: SEX : DOSE GROUP: 115 Female Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen A n h a l No: 116 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen days of treatment : 28 . sacrifice group : terminal, sacrifice necropsy status : planned tenalnal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/Z, oMry/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days df treatment : 28 . sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (-1) Hemosiderin pigment (slight) BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER AGCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Pcopath no. : 99010 Date : 21 .APE. 1999 Individual A n i m a l D a t a Records Animal No: SEX: DOSE GROUP: 116 Female Telaer Alcohol MICROSCOPIC FINDINGS (continued) A n i m a l No: 117 MACROSCOPIC FINDINGS Kidneys MICROSCOPIC FINDINGS Liver Kidney/Z Adrenal G l a n c U l Spleen No abnormalitfes found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : tenainal sacrifice necropsy status : planned tendrial date of.start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 right side Pelvic dilation No abnormalities were found in any of the other tissues examined Inflammatory cell f o c i (minimal) Pelvic dilation present (correlates to GROSS finding) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (mi-) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, KidneY/l, OMry/l, Ovary/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, ' M a n d i b u l a r Lymph Node/2,. . , BACK TO MAIN --._- Test Article: TELOMERALCOHOL/TELCMER ACRYLATE/ ,/PFHs/pFos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 76/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 118 Female Telcaner AkohOl MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Thymus Mandibular Lymph Node/ 1 Animdl No: . 119 I ( MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned t e b a l date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date.of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) Plasmacytosis (moderate) NO abnormalities found in: Pancreas, ICidney/l, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER AI.COHOL/TEL@~ER ACRYLATE/ . /PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 77/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records A n i m a l No: SEX: DOSE GROUP: 119 Female Telmer Alcohol MICROSCOPIC FINDINGS Liver Kidney/2 Spleen Animal No: 120 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Inflammatory cell foci (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) Lymphocytolysis, increased (minimal) No abnormalities found in: Pancreas, Kidney/l, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : teminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal1 Hemosiderin pigment (slight) No abnomlities found in: Pancreas, Kidney/lt Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT -_-_.- Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services * Page 78/146 NOTOX no. : 242933 Ptopath no.: 99010 Date : 21 .Apr. 1999 Individual hnimal. Data Records Animal No: SEX: DOSE GROUP: 121 Female Telcrmer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS A n b a l No: 122 MACROSCOPIC FINDINGS Skin MICROSCOPIC FINDINGS Skin A n i m a l No: 123 MACROSCOPIC FINDINGS I days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date o f necropsy : 20/11/98 Alopecia No abnormalities were found i n any of the other tissues examined No micro correlate days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 BACK TO MAIN PATHOLOGY REPORT --.-_- Test Article: TELQMER ALCOHOL/TELOMER ACRYLATE/ ./PMS/PFOs Test System :'Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 79/146 NOTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual 2 i d m a l D a t a ICeCOrds An- No: SEX: DOSE GROUP: 123 Female Telcrmer Alcohol MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Animat No: 125 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 126 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/90 No findings noted No tissues examined BACK TO MAIN PATIIOLOGY REPORT Test Article: TELWER XCOHOL/?ELOMER ACRYLATE,' /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 80/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr. 1999 Individual A n i m a l Data R e c o d Animal No: SEX: DOSE GROUP: 127 Female T d o m r Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: DOSE GROUP: 33 We Telomer Acrylat MACROSCOPIC FINDINGS h . MICROSCOPIC FINDINGS Liver Pancreas ICLdney/l Mdney/2 Spleen days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis ( m i n i m a l ) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) BACK TO MAIN PA'I'XOMGY REPORT --._- Test Article : TELOMER ALCOHOL/TELWER ACRYLATE/ f PE'HSf PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 81/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual AnimdL D a t a Records Animal No: SEX: DOSE GROUP: 33 Mal e Telomer Acrylaf MICROSCOPIC FINDINGS (continued) Animal No: 34 MACROSCOPIC FINDINGS . MICROSCOPIC FINDINGS Liver IUdney/l Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Number of Sections less than protocol for Mandibular Lymph Node/l (0). No abnormalities found in: Testesll, Testes/2, Adrenal Gland/l, Adrenal Gland/P, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/Z. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Interstitial inflammation, lymphocytic (&&mal) Hemapoiesis, primarily erythropoiesis (minimal) Plasmacytosis (slight) Plasmacytosis (slight) NO abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node. BACK TO MAIN PATHOLOGY REPORT Test Article: TELWER ALCOHOLITELOMER ACRYLATE/ lPFHsl~F0s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 82/146 NOTOX no. : Propath no.: 242933 99010 Date : 21.Apr.1999 Animal No: SEX: DOSE GROUP: 35 Male Telcmrer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 36 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen days of treatment : 28 sacrifice group : tenuinal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflarmaatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis ( m i n h t d . ) No abnormalities found in: Pancreas, Kidney& Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, mendl Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis ( m i n i m a l ) Tubular atrophy, cortical (mMmal.1 Interstitial inflaxnation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (mi-) BACK TO MAIN PATHOLOGY REPORT ---- Test Article: TELOMERALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 831146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 36 Male Tehmer Acrylat MICROSCOPIC FINDINGS (continued) . v. Animal No: 37 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver I .. . i , ICldney/l Spleen Mandibular Lymph Node/ Number of Sections less than protocol for Mandibular Lymph Node/l ( 0 ) . No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lynp?h Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned t e d n a l date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (moderate) single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, a(idneY/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT --._- T e s t Article : TELOHER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t system : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 84/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 No: SEX: DOSE GROUP: 38 Male Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal NO: 39 '... . ' MACROSCOPIC FINDINGS days 02 treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) Plasmacytosis (slight) NO abnormalities found in: Pancreas, Kidney/l, adneY/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : terninal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT --.. .& Page 85/146 Test Article: T E L ~ E RA~coHOL/~ELOMEARCRYLATE/ IPFHS/PFOS NOTOX no. : 242933 Test System : Exploratory 28-Day Gavage Study in Rats ' Propath no.: 99010 Sponsox : 3M Toxicology Services Date : 21.Apr.1999 Individual Animal Data Recorda Animal No: SEX: DOSE GROUP: 39 Male Telomer Acrylat MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necxosis (minimal) Pancreas Kidney/l Apoptosis/single cell necrosis, exocrine (minimal) Exocrine atrophy, focal (minimal1 Tubular atrophy, cortical (minimall Pelvic inflamnation, lymphoid (minimal) Spleen Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Kidney/2, Testedl, Testes/Z, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. Animal No: 40 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : tennindl ~acrifice necropsy status : planned t e d n a l date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 . .. MICROSCOPIC FINDINGS No findings noted Liver Inflammatory cell foci (slight) Kidney/l Tubular atrophy, cortical (mtnimdl) Spleen Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Thymus Lymphocytolysis, increased (slight) Mandibular Lymph Node/ 1 Congestion/erythrophagocytosis (moderate) BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELWR ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 314 Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records A n i m a l No: SEX: DOSE GROUP: 40 Male TelOmeK Acrylat MICROSCOPIC FINDINGS (continued) Animal No: 41 , . MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS A n b a l No: 42 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/Z, Adrenal Gland/l, Adrenal Gland/ 2, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy : 18/11/90 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of s t a r t of treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy : 18/11/98 No findings noted NO tissues examined BACK TO MAIN PATIloMGY REPORT Test Article: TELwR ALCOHOLITELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.24pr.1999 Indiddual A n i m a l Data Records h h a l No: SEX: DOSE GROUP: 43 Male Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 45 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS . . .. A n h a l . No: 46 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date o f start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : Prbpath no.: Date : . 242933 99010 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 46 Male Telomer Acrylat MICROSCOPIC FINDINGS *, ... ..... ... : ANLmdl No: 47 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: 129 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen ..No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : other date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (slight1 Hemosiderin pigment (minimdl) BACK TO MAIN PATIlOMGY REBORT --c1 T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/ PFOS T e s t System : Exploratory 28-Day Gavage study in R a t s Sponsor : 3M Toxicology Services Page 89/146 NOTOX no. : 242933 Pfopath no.: 9901'0 Date : 21.Apr. 1999 Individual A n i m a l D a t a Records Adanal No: SEX: DOSE GROUP: 129 Female Telamer Acrylat MICROSCOPIC FINDINGS (continued) Adanal No: 130 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KYdney/l KYdney/z Spleen No abnormalities found in: Pancreas, Kidney/l, Kidney/Z, OMry/l, Ovary/2, Adrenal ~land/l, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. day:, of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflaxmatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) Tubular atrophy, cortical (minimal) Tubhat atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid - a t ~ ~ p h yinvolution (mMmdl) No abnormalities found in: Pancreas, OMry/l, Ovary/ 2, Adrenal Gland/l, Adtendl Gland/2, Mesenteric Lymph Node, Mandibular LvIllph Node/l, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY RERORT Test Article: TELOMERALCOHOL/TELOMER ACRYLATE/ -/PFHs/PFOs Test System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l Data Records A n h a l No: SEX : DOSE GROUP: 131 FamdLe Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 132 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : tenaind sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, 0vary/2, Adrenal ~land/l, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PA!PHOLOGY REPORT ---_- Test Article : TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 91/146 NOTOX no. : 242933 Piopath no.: 99010 Date : 21 .Apr. 1999 Individual Animal Data Records AnimdL No: SEX: DOSE GROUP: 132 Female Telomer Acrylat MICROSCOPIC FINDINGS Liver Pancreas Spleen Animal No: 133 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS' Liver Spleen Inflammatory cell foci (minimal) Exocrine atrophy, focal (minimal) Hemopoiesis, primarily erythropoiesis (dnirnal.) Hemosiderin pigment (minimal) NO abnormalities found in: Kidney/l, KLdney/S, Ovary/l, Ovary/2, Adrenal Gland& Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflanrmatory cell foci (slight) Single cell necrosis (slight) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) No abnormalities found in: Pancreas, Kidney/l, KidneyD , -ry/l , 0vary/2, Adrenal Gland/lI Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT -. _ C _ Test Article: TELOHER FiLCOHOL/TELOMER ACRYLATE/ /PE'HS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 92/146 NOFOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 134 Female Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Ictdney/2 Spleen Animal No: 135 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : tenninal sacritifice necropsy status : planned teminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemapoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphocytolysis, increased (minimal) No abnormalities found in: Pancreas, Kidney/l, Ovary/l, ovary/P, Adrenal Gland/l, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned te&al date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT -- .._- Test Article: TELOMER ALCOHOL/TELCMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 93/146 NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individwl A n i m a l Data Records Animdl. No: SEX : DOSE GROUP: 135 Female Telamer Acrylat MICROSCOPIC FINDINGS Liver spleen Mandibular Lymph Node/ 1 Animdl No: 136 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/2 Adrenal Gland/l Spleen Thymus Mandibular Lymph Node/ 1 Inflamatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis ( m i n i m a l ) Hemosiderin pigment (slight) Plasmacytosis (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/Z, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : tenainal sacrifice necropsy status : planned t e d n a l date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Interstitial inflammation, lymphocytic (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) Lymphoid atrophy - involution (minimal) Plasmacytosis (slight) BACK TO MAIN PA!I!HOLOG!i REPORT --*_A T e s t Article: TELCNER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services Page 94/146 NOTOX no. : 242933 Pmpath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l Data Records Animal No: SEX: DOSE GROW: 136 Female Telmer Acrylat " MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 2 Animal No: 137 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 138 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Lyqhoid --yperplasia(slis--t) No abnormalities found in: Pancreas, Kidney& Ovary/l, Ovary/2, Adrenal Gland/P, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No.-tissueS examined BACK TO MAIN --L- PATHOLOGY REPORT T e s t Article: TELCMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology S e r v i c e s Page 95/146 NqTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 I d v i d u a l Animal D a t a Records A n i m a l No: SEX: DOSE GROUP: 139 Female Telomer Acrylat MACROSCOPIC FINDINGS days of treatment : 29 s a c r i f i c e group : recovery group necropsy status : planned recovery date of start o f treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted MICROSCOPIC FINDINGS No t i s s u e s examined AnimdL No: 141 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start o f treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted MICROSCOPIC FINDINGS .. Animdl No: 142 No tissues examined MACROSCOPIC FINDINGS days of treatment : 29 s a c r i f i c e group : recovery group 2 necropsy status : planned recovery 2 date of s t a t of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 NO findings noted BACK TO MAIN RATIOLOOY REBORT ----I Test Article: T E L M R ALCOHOL/TEmR ACRYLATE/ ,/PFHS/BFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 96/146 NOTOX no. : 242933 PYopath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records k d m a l No: SEX: DOSE GROUP: 142 Female Telcaner Acrylat MICROSCOPIC FINDINGS Animal No: 143 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: DOSE GROUP: 49 Male MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/90 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 28 sacrifice group : termindl sacrifice necropsy status : planned tenninal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (mi-) BACK TO MAIN PATHOLOGY REPORT Test Article: TELOHER XCOHOL/TELOMER ACRYLATE/ = i/PF?IS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE $ROUP: 49 Male MICROSCOPIC FINDINGS (continued) Animdl No: 50 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen A n h a 1 No: 51 MACROSCOPIC FINDINGS No abnormalities found in: Pancreas, KYdney/2, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : tenainal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (0linima.l) Hemopoiesis, primarily erythropoiesis ( m i n i m a l ) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Testas/l, Testes/2, Adrand Gl=d/l, Adrenal Gland/P, Thymw, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : termindl sacrifice necropsy status : planned t e d n a l date of start of treatment: 06/10/98 . date of end of treatment: 02/11/98 date of necropsy : 03/11/98 BACK TO MAIN PAT BOLO^ REPORT Test Article: TELCPlER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory' 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 98/146 NO.TOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 51 We MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Spleen Animal No: 52 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mesenteric Lymph Node Mandibular Lymph Node/ 1 Mandibular Lvmph Node/ 2 No findings noted Inflammatory cell foci ( m i n i m a l ) .. Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnonnalities found in: Pancreas, Kidney/l, Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted relamamtory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal1 Hemosiderin pigment (minimdl) Congestion/eqthrophagocytosis (minimal) Plasmacytosis (slight) Lymphoid hyperplasia (slight) Congestion/erythrophagocytosis (slight) Congestion/erythrophagocytosis (slight) BACK TO MAIN PATHOLOGY =PORT T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology S e r v i c e s NQTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 . ., ; ' ,': . ..' . . i Individual Animal D a t a Records Animal No: SEX : DOSE GROUP: 52 Male MICROSCOPIC FINDINGS (continued) AnimdL No: 53 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animal No: 54 MACROSCOPIC FINDINGS No abnormalities found in: Pancreas, KFdney/l, Kidney/Z, Testes& Testes/Z; Adrenal Gland/l, Adrend. Gland/2, Thymus. days of treatment : 29 s a c r i f i c e group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory c e l l foci ( s l i g h t ) Tubular atrophy, cortical (minimal) I n t e r s t i t i a l inflaxtunation, lymphocytic (ddmal) Hemopoiesis, primarily erythropoiesis ( s l i g h t ) Hemosiderin pigment (minimal) NO abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/Z, Adrenal G l a n d / l , Adtenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular L w h Node/l, Mandibular Lymph Node/2. days of treatment : 29 s a c r i f i c e group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 BACK TO MAIN --L1 PATHOLOGY REPORT T e s t Article: TELOMER AI,COHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 100/146 NOTOX no. : 242933 Prhpath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records No: SEX: DOSE GROUP: 54 Male MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animdl No: 55 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen Mandibular Lymph Node/ 1 No findings noted Inflammatory cell foci (slight) Single cell necrosis (nd.nimal) Hyaline cast(s) (minimal) liemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/S, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : t e d n a l sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Apoptosis/single cell necrosis, exocrine (slight) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) PlaJmacytOsfs (slight) BACK TO MAIN PATHOLOGY REPORT --._ .C Page 101/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS NOTOX no. : 242933 ' Test System : Exploratory 2 8 - D ~Gavage Study in Rats Pgopath no.: 99010 Sponsor : 3M Toxicology SeSViCeS Date : 21.Apr.1999 Individual Animal D a t a Records A n h l No: SEX: DOSE GROUP: 55 Male MICROSCOPIC FINDINGS (continued) Mandibulaz Lymph Node/ 2 Animal No: 56 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Kidney/2 Adrenal Gland/l Spleen P1asmacytosis (slight) No abnormalities found in: I(idney/l, Wdney/2, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lyraph Node. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/90 No findings noted Inflammatory cell foci (minimal) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hyaline cast(s) (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) - Lyqhoid atrophy involution (minimal) Lyqhocytolysis, increased (slight) No abnormalities found in: Pancreas, Testedl, Testes/Z, Adrenal Gland/2, Mesenteric Lymph Node, Mandibulax Lymph Node/l, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOOY REPORT ---A Page 102/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHs/pFos Test System : Exploratory 28-Day Gavage Study in Ra'ts Sponsor : 3M Toxicology Services NOTOX no. : 242933 Pfopath no.: 99010 Date : 21.Apr.1999 Individual Anixnal Data Records A n h l No: SEX: DOSE GROUP: 57 Mal e MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 58 MACROSCOPIC FINDINGS 1% : MICROSCOPIC FINDINGS Animdl No: 59 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy : 18/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group ' necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice gtoup : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necsopsy : 18/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT --I_- Page 103/146 Test Article: TELCMER ALCOHOLITELWER ACRYLATE/ JPFHS/PFOS Test System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records A n i m a l No: SEX: DOSE GROUP: 59 Male MICROSCOPIC FINDINGS Animal No: 61 MACROSCOPIC FINDINGS Eyes MICROSCOPIC FINDINGS Eyes An- No: 62 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 lens: right side Reduced in size No abnormalities were found in any of the other tissues examined Periorbital inflammation due to blood sampling (moderate) (correlates to GROSS finding) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined BACK TO MAIN PA'EIOLOGY REPORT ---_- Test A K t i C l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ '/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 104/146 NOTOX no. : 242933 Pr'opath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX : DOSE GROUP: 63 We MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: 145 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined days of treatment: : 28 sacrifice group : tenuinal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight) NO abnormalities found in: Pancreas, Kidney/l, fi&eY/2, OMry/l, Ovary/2, Adrend Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN -4 Page 105/146 Test Article : TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NqTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Anilnal No: SEX: DOSE GROUP: 146 Female MACROSCOPIC FINDINGS - .. MICROSCOPIC FINDINGS Liver Spleen Animal No: 147 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : tenninal sacrifice necropsy status : planned terminal date of start of treatxnent: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/Z, Thyrmts, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELCMER ACRYLATE1 /PFHS/PFCS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 106/146 NO-TOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 147 Fde MICROSCOPIC FINDINGS Liver Kidney/l Spleen Mandibular Lymph Node/ 1 Animdl No: 148 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen ' Thymus Inflammatory cel foci (dILMl) single cell-necrosis (minimal) Cystic tubules (slight) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Xidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Interstitial inflanuuation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis ( m h b a l ) Hemosiderin pigment (slight) Lymphocytolysis, increased (minimal) No abnormalities found in: Pancreas, Kidney/Z, oMZy/l, Ovary/Z, Adrenal Gland/l, Adrenal Gland/z, Mesenteric Lymph Node, M a n d i b u l a r Lymph Nodell, Mandibular Lymph Node/2. BACK TO MAIN ----- PATHOLOGY REPORT Test Article: TELOMERALCOHOL/TELCMER ACRYLATE/ JPFHWPE'Os Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 107/146 NOTOX no. : 242933 Piopath no.: 99010 Date : 21.Apr.1999 Individual Aaimal Data Records Animal NO: SEX: DOSE GROUP: 149 Fa18 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 150 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted ' Inflammatory cell f o d (slight) Hemapoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : t e h a l sacrifice necropsy status : planned t e d n a l date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Hematopoietic cell foci (minimal) Hemosiderin pigment (moderate) Plasmacytosis (slight) Lymphoid hyperplasia (slight) BACK TO MAIN PATHOLOGY REPORT --_- Page 108/146 Test Article: TELOMER ALCOHOL/TELMER ACRYLATE/ /PFHS/PFos Test System : Exploratory 28-Day Gavage Study in Rats' Sponsor : 3M Toxicology Services NOFOX no. : Propath no.: Date : 242933 99010 21.*r.1999 IndivAdual Animal D a t a Records Animdl No: SEX: DOSE GROUP: 150 Female MICROSCOPIC FINDINGS ( continued) Animal No: 151 MACROSCOPIC FINDINGS Ute- MICROSCOPIC FINDINGS Liver uterus ,. ' Spleen No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, Wary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : tenninal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 left side Cyst (s) No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Cyst(s) present sinple serosal) (correlates to GROSS finding) Hemosiderin pigment ' (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, Wa.ry/Z, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. BACK TO MAIN Test Article: TELQMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System' : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 109/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records Animal No: SEX: DOSE GROUP: 152 remde MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver fidney/l Spleen Animal No: 153 .. ..... M7LCROSCOPIC FINDINGS MICROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status, : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci . (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal1 Heraopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) NO abnormalities found in: Pancreas, Kidneyh, OMry/l, mary/2, Adrenal Gland/l, Adrenal G l a n d / Z , Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT -?.-- Test Article: TELOMER F&COHOL/TELWR ACRYLATE/ -/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 314 Toxicology Services Page 110/146 NOTOX no. : 242933 Propath no'.: 99010 Date : 21.Apr.1999 Individual Animal Data Records AnFmdL No: SEX: DOSE GROUP: 154 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animdl NO: 155 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 157 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 00/10/98 date of end of treatment: 05/11/90 date of necropsy : 20/11/90 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 00/10/98. date of end of treatment: 05/11/98 date of necropsy : 20/11/90 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 00/10/90 date of end of treatment: 05/11/90 date of necropsy : 04/12/98 No findings noted BACK TO MAIN Page 111/146 T e s t Article : TELWER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory'28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services NQTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records No: SEX: DOSE GROUP: 157 Female MICROSCOPIC FINDINGS ?mhal No: 158 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS AnimdL NO: 159 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy statw : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 112/146 NOTOX no. : 242933 Propath no.: soia Date : 21.Apr. 1999 Individual A d a d . Data Records Aniraal No: SEX: DOSE GROUP: 65 Male PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Mdney/l Spleen AnimdL No: 66 .._... MACROSCOPIC FINDINGS Lungs Staach Testes days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/90 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertropby, diffuse (moderate) Hepatocellular vacuolation (slight1 Tubular atrophy, cortical (minimal Hemopoiesis, primarily erythropoiesis (slight) No abnormalities found in: Pancreas, mCidney/Z, Testedl, Tasted2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular L m h Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 Hemorrhage glandular mcosa Hemorrhage Reduced in size BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ f PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 113/146 NQTOX no. : 242933 Propath no.: 99010 Date : 21.Fpr.1999 IndividudL U D a t a Records Animal No: SEX: DOSE GROUP: 66 Mala PPHS MACROSCOPIC FINDINGS (continued) Epididymides . .. i t MICROSCOPIC FINDINGS Lungs Stomach Liver ! .. : ' Pancreas Kidney/l Tastedl Tested2 Epididymides Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Reduced in size No abnormalities were found in any of the other tissues examined Intra-alveolar blood, necropsy artefact present (correlates to GROSS finding) Vascular mineralisation, focal (minimal) No micro correlate Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Seminiferous tubular atrophy (severe) (correlates to GROSS finding) Seminiferous tubular'atrophy (severe) (correlates to GROSS finding) Atrophy (moderate) (correlates to GROSS finding) Aspermia present Hemopoiesis, primarily erythropoiesis (raf.nbdl) Hemosiderin pigment (minimal) Plasmacytosis (moderate) Congestion/erythrophagocytosis (minimal) No abnormalities 'found in: Kidney/2, Adrenal G l a d / 1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node. ~~ - BACK TO MAIN PATHOLOGY REPORT --._- Test Article: TELCUER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services ~ Page 114/146 NQTOX no. , : Propath no.: Date : 242933 99010 21.Apr.1999 Individual A n i m a l Data Records Anhal No: SEX: DOSE GROUP: 67 Male PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen Mandibular Lymph Node/ 1 A n h a l No: ' 68 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned tenatnal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (minimal) Coagulative necrosis, focal (minimal) Tubular,atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Plasmacytosis (slight) NO abnonnalities found in: Pancreas, Kidney/2, Testes& Testes/2, Adrenal GLand/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 20 sacrifice group : termFndl sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/90 No findings noted . BACK TO MAIN PATHOLOGY =PORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/' . a/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 115/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr. 1999 Individual A n i m a l Data Records Animal pro: SEX: DOSE GROUP: 68 Male PFHS MICROSCOPIC FINDINGS .. ._. ,. Pancreas Kidney/l Spleen Animdl No: 69 MACROSCOPIC FINDINGS 6 , MICROSCOPIC FINDINGS Liver Kidney/l Adrenal Gland/l Spleen . Inflammatory cell foci (slight) MidzonaIJcentrilobular hypertrophy, diffuse (moderate) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic ( m i n i m a l ) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: lUdney/2, Testes/l, Testes/Z, Adrenal Gland/l, Adrenal Gland/Z, Thymus, Mesenteric Lynph Node, Mandibulac Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned t e d n a l date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell f o c i (slight) Single cell necrosis (minimal) MidzonaIJcentrilobular hypertrophy, diffuse (moderate) Interstitial inflanrmation, lymphocytic ( d f i m l ) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) BACK TO MAIN PATHOLOGY REPORT -"'-- Test Article : T E L ~ E RAGCOHOL/TELOP.IER ACRYLATE/ .`PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : JM Toxicology Services Page 116/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr. 1999 Individual Animal Data Raoords Anisldl No: SEX: DOSE GROUP: 69 Male PFHS MICROSCOPIC FINDINGS (continued) Animal No: 70 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas I . . ... Kidney/l Spleen No abnonualities found in: Pancreas, Kidney/2, Testedl, TestesI2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Ncde/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Inflammation, mixed granulocytic/lymphocytic (minimdL) Interstitial. inflannnation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (slight1 No abnormalities found in: Kidney/2, Testss/l, Tested2, Adrenal Gland/l, Adrenal Gland/2, T h F s , Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. BACK TO MAIN PATHOLO(jY REPORT -_._- Test Article : TELOMER ALCO!IOL/TELOUER ACRYLATE/ ,'PE'HS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 117/146 NOFOX no. : 242933 Propath'no.: 99010 Date : 21.Apr.1999 Individual Auimal D a t a Records Animal No: SEX: DOSE GROUP: 71 Male PFHS MACROSCOPIC FINDINGS days of trea-ent : 29 sahrifice group : termina sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 'date of necropsy : 04/11/98 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minimal) Kkkonal/centrilobula.r hypertrophy, diffuse (slight) ECidney/l Cystic tubules (slight) Spleen Hempoiesis, primarily erythropoiesis (minimdl) Hemosiderin pigment (minimal) Mandibular Lymph Node/ 1 Plasmacytosis (slight) Mandibular Lymph Node/ 2 Plasmacytosis (slight) , ! I a. Animal No: 72 No abnormalities found in: Pancreas, ICidney/l, Testes/l, Testes/P, Adrenal Gland/l, Adrenal G l a n d / 2, Thymus, Mesenteric Lymph Node. MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : tenainal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 Liver Enlarged BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TEL(MER ACRYLATE/ ;/PMS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 118/146 NOTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 72 Mal e PFHS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 73 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) (correlates to GROSS finding) Hepatoce11ula.r vacuolation ( ~ ~ l ) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight) Plasmacytosis (slight) Congestion/erythrophagocytosis (slight) Congestion/erythrophagocytosis (minimal) NO abnormalities found in: Pancreas, Kidney/l, Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted Inflammatory cell foci (moderate) Single cell necrosis (slight) Mi.dzonal/centrilobular hypertrophy, diffuse (slight) BACK TO MAIN PATIIOLOGY REPORT -.--__ Test Article: TEL~MERALCOHOL/TELOMER ACRYLATE/ /PFHs/PE'os Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 119/146 NQTOX no. : 242933 Propath no.: 99010 Date : 21.Apr. 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 74 Male PFHS MACROSCOPIC FINDINGS Testes MICROSCOPIC FINDINGS Liver Testes/l Testes/2 Animal No: 75 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment : 29 sacrifice group : recovery group necropsy statu3 : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 Reduced in size No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Seminiferous tubular atrophy (moderate) (correlates to GROSS finding) Seminiferous tubular atrophy (moderate) (correlates to GROSS finding) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/90 No findings noted Inflammatory cell f o d (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) BACK TO MAIN PATXOLOGY REPORT T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ i /PFHS/PFOS T e s t System : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology S e r v i c e s Page 120/146 NQTOX no. : Propath no.: Date : 242933 99010 21 .Apr. 1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 77 Male PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 78 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver An- No: 79 MACROSCOPIC FINDINGS days of treatment : 29 s a c r i f i c e group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory c e l l f o c i ( s l i g h t ) Single c e l l necrosis (minimal) days of treatment : 29 s a c r i f i c e group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 . date of necropsy : 02/12/98 No findings noted Inflammatory c e l l foci' (slight) Single cell necrosis (minimal) days of treatment : 29 s a c r i f i c e group : recovery group 2 necropsy status : planned recovery 2 date of start o f treatment: 06/10/98 date of end of tieatment: 03/11/98 date of necropsy : 02/12/98 BACK TO MAIN PATHOLOGY REPORT ----- Test &tide: TELOMER ALCOHOL/TELWR ACRYLATE; /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 121/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records A n a l No: SEX: DOSE GROUP: 79 Male PFHS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS . .. Liver A n i m a l No: SEX: 161 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen No findings noted Inflammatory cell foci (slight) Hepatocellulax vacuolation (minimal) days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Tubular epithelial hypertrophy, focal (-1) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/Z, OMry/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT -_c_ Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PEOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 122/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Anixual Data Records Animal No: SEX: DOSE GROUP: 162 Female PFHS . ' MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen A n i m a l No: 163 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned telminal date of s t a t of treatment: 08/10/98 date of end of treatment: 04/11/90 date of necropsy : 05/11/98 No findings noted Inflammatory cell f o d (slight) Single cell necrosis (minimal) , Hemopoiesis primari1y erythropoies1s (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 . sacrifice group : terminal sacrifice necropsy status : planned telJnina1 . date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PATROLOOY =PORT -?-_& Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PEHS/PEOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 123/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records 1 - NO: SEX: DOSE GROUP: 163 Female PFHS MICROSCOPIC FINDINGS Liver Spleen Thymus Mandibular Lymph Node/ Inflammatory cell foci (moderate) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight) Lpphocytolysis, increased (minimal ) Plasmacytosis (slight) Animal No: 164 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen No abnonndlities found in: Pancreas, Kidney/l, KidneY/Z, oMry/1, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned t e d n a l date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (slight) No abnormalities found in: Pancreas, Kidney/l, KidmY/Z, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT _La Test ZVtiCle: TELWER ALCOHOL/TELONER ACRYLATE/ -/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 124/146 NQTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records Animal No: SEX: DOSE GROUP: 165 Female PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen A n i m a l No: 166 MACROSCOPIC FINDING days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Ktdney/l, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 s a u i fice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN --cs PATHOLOGY REPORT T e s t AEtiCle: TELOHER ALCOHOL/TELOWER ACRYLATE/ 'PMS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsoz : 3M Toxicology Services Page 125/146 NQTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual A n i m a l Data Records Animal No: SEX: DOSE GROUP: 166 Female PE'HS MICROSCOPIC FINDINGS Liver Inflammatory cell foci (minimal) Adrenal Gland/l Extracapsular nodule present Spleen Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Mandibular Lymph Node/ 1 Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/l, Kidney/2I oMry/l Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. Animal No: 167 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 MICROSCOPIC FINDINGS '. . Liver No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Spleen Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (alight) NO abnormalities found in: Pancreas, Kidney/l, Kidney/?, OMry/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Handibulat Node/2- BACK TO MAIN PATHOLOOP REPORT Test A K t i C h : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOx no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individnal Animal. D a t a Records Animal No: SEX: DOSE GROUP: 168 Female PFHS MACROSCOPIC FINDINGS .. .. MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animal No: 169 MACROSCOPIC FINDINGS ! MICROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) Cystic tubules ( m i n i m a l ) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, KCdney/2, Ovary/l, OVary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/;!. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy . : 20/11/98 No findings noted '. No tissues examined BACK TO MAIN PATHOLOG% REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal. Data Records A n i m a l No: SEX: DOSE GROUP: 170 Fcmdle PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS A n i m a l No: 171 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS A n i m a l No: 173 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/l2/98 No findings noted BACK TO MAIN -_.-- Test Article: TELOMERALCOHOL/TELOMER ACRYLATE/ ./ems/PFos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 128/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records No: SEX: DOSE GROUP: 173 Female PFHS MICROSCOPIC FINDINGS Animal. No: 174 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 175 MACROSCOPIC FINDINGS ;.. MICROSCOPIC FINDINGS No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date-of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT -*._1 T e s t Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS T e s t System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 129/146 NQTOX no. : 242933 Propath no.: 99010 Date : 21.Apr. 1999 Individual Animrrl Data Records h h a l No: SEX: DOSE GROUP: 81 Male PFOS MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted MICROSCOPIC FINDINGS Liver Inflammatory cell foci (slight) Single cell necrosis (minirnal) W.dzonaJJcentri1obula.r hypertrophy, diffuse (slight) Hematopoietic cell foci (minimal) Kidney/l Spleen Tubular atrophy, cortical (minimal1 Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Mandibular Lymph Node/ 1 Plasmacytosis (moderate) I. l h h a l No: 82 No abnormalities found in: Pancreas, KidneY/P, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular LVmph Node/2. MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : tenninal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted BACK TO MAIN PATIIOLOGY REPORT Test Article: TEL-R ALCOHOL/TELOMER ACRYLATE/ /PEHS/PFOS Test System : Exploratory 20-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 130/146 NQTOX no. : 242933 Propath no.: ' 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 82 Male PFOS MICROSCOPIC FINDINGS Liver Pancreas ICidney/l Spleen 1 - No: 83 MACROSCOPIC FINDINGS t. . MICROSCOPIC FINDINGS Liver Kidney/l Kidney/2 Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellulaz vacuolation (minimal) Apoptosis/single cell necrosis, exocrine (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) No abnormalities found in: Kidney/2, Testedl, Testes/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : tenainal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (minimal) Interstitial inflanunation, lymphocytic (alight) Cystic tubules (slight) Interstitial inflammation, lymphocytic (minimal) BACK TO MAIN PATHOLOOY REPORT Test Article: TELOMER FU.COHOL/TELOMER ACRYLATE/ .i -';/PFHS/PFOs Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 131/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records l h h a l No: SEX: DOSE GROUP: 83 Male PFOS MICROSCOPIC FINDINGS (continued) Tested1 Spleen Animdl No: 84 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Adrenal Gland/l Adrenal Gland/2 Spleen Mandibular Lymph Node/ 1 Spenaatidic giant cell(s) (mMmal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal1 No abnormdlities found in: Pancreas, Testes/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node12. days of treatment : 20 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) MidzonaUcentrilobular hypertrophy, diffuse (moderate) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (d-1) Atrophy, cortical diffuse (slight) Atrophy, cortical diffuse (slight) Hemopoiesis, primarily erythropoiesis (mirzimdl) Plasmacytosis (slight) BACK TO MAIN PATBOLOGY REPORT -?.-- Page 132/146 Test Article: TELdMER N.COHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animdl No: SEX : DOSE GROUP: 04 Male PFOS MICROSCOPIC FINDINGS (continued) Animal No: 05 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver ?Sdney/l Spleen Animal No: 86 MACROSCOPIC FINDINGS No abnormalities found in: Pancreas, Kidney/2, Testes& Testes/2, Thynuas, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimdl1 No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : tenuinal sacrifice necropsy status : planned tenuinal date of start of treatment: 06/10/98 date of end of treatment: 03/11/90 date of necropsy : 04/11/98 BACK TO MAIN Test Article: TELOMER ALCOHOLfTELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 1331146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual A n i m a l D a t a Records AnimdL No: SEX: DOSE GROUP: 86 Male PM)S MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS .. . . Liver fidney/Z Adrenal Gland/l Adrenal Gland/P Spleen Thw A n h a l No:. 87 MACROSCOPIC FINDINGS No findings noted Inflammatoa cell foci (slight) Single cell necrosis (minimal) Midzonal/centrllobular hypertrophy, diffuse (slight) Hepatocellular vacuolation (minimal) Tubular atrophy, cortical (minimal) Hyaline cast(s) (minimal) Tubular atrophy, cortical ( m i n i m a l ) Vacuolation, multifocal in z. fasiculata (minimal) Vacuolation, multifocal in z. fasiculata (minimal) Hemapoiesis, primarily erythropoiesis (slight) Lymphoid atrophy - involution (slight) No abnormallties found in: Pancreas, Testes& Testes/P, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of neczopsy : 04/11/98 No findings noted BACK TO MAIN --L- PATIOMGY REPORT Test Article : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 134/146 NOTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Indiddual Animal Data Records Animal No: SEX : DOSE GROUP: 87 Male PFOS MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animal No: 88 MACROSCOPIC FINDINGS . .. MICROSCOPIC FINDINGS Liver Pancreas Adrenal Gland/l Adrenal Gland/2 Inflammatory cell foci (slight) Single cell necrosis (minimal) Mdzonal/centrilobulax hypertrophy, diffuse (moderate) Hepatocellular vacuolation (slight) Tubular atrophy, cortical ( m i n i m a l ) Hyaline cast(s) (minimal) Hemopoiesis, primarily erythropoiesis (minimal) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/Z, Adrenal Gland/l, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : t e d n a l sacrifice necropsy statu$ : planned terminal date of s t a r t of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Apoptosis/single cell necrosis, exocrine ( m i n i ~ ~ l ) Vacuolation, multifocal in z. fasiculata (minimal) Vacuolation, multifocal in z. fasiculata (minimal) BACK TO MAIN PATHOLOGY REPORT Page 135/146 Test Article: TELCMER ALCOHOL/TELOMER ACRYLATE/ /PE'HS/PFOS NOTOX no. : Test System : Exploratory 28-Day Gavage Study in Rats ' Propath no.: Sponsor : 3M Toxicology Services Date : 242933 99010 21.Apr.1999 Individual Animal Data Records Animdl No: SEX: DOSEGROUP: 88 Male PEW MICROSCOPIC FINDINGS (continued) Spleen ICdmal No: 89 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver t 1 Animal No: 90 MACROSCOPIC FINDINGS Liver Hemopoiesis, primarily erythropoiesis ( m i n i m a l ) Hemosiderin pigwmt (minimal) Lymphoid atrophy - involution (minimal) No abnormalities found in: Kidney/l, I(idney/2, Testes/l, Testes/2, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/Z. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzondl/centrilolwla.r hypertrophy, diffuse (slight) Hepatocellular vacuolation (mM.mal) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 Accentuated lobular pattern BACK TO MAIN PATHOLOGY RE2ORT Test Article: TELQMER ALCOHOL/TELWR ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3~ Toxicology Services Page 136/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 90 Male PEW MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Animdl No: 91 MACROSCOPIC FINDINGS MICROSCOP1C FINDINGS i Liver Animal No: 93 MACROSCOPIC FINDINGS N o abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) MidzonaVcentrilobular hypertrophy, diffuse (moderate) (correlates to GROSS finding) Hepatocellular vacuolation (slight) (correlates to GROSS finding) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 N o findings noted Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (slight) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 BACK TO MAIN PATHOLOOP REPORT Test Article: TELWR ALCOHOL/TELOMER ACRYLATE; ;PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 137/146 NOTOX no. : 242933 Propath no.': 99010 Date : 21.Apr.1999 Individual Animal. Data Records Animdl No: SEX: DOSE GROUP: 93 Male PFOS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver AnimdL No: 94 MRCROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver . , Animal No: 95 MACROSCOPIC FINDINGS No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) ~dzonal/centrilobularhypertrophy, diffuse (minimal1 days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted BACK TO MAIN PATHOLOGY REPORT --L1 Test Article: TELOMER ALcoHOL/TELWR ACRYLATE/ .'PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 138/146 NQTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual Atlimal Data Records AN$adl No: SEX: DOSE GROUP: 95 Male PFOS ' MICROSCOPIC FINDINGS Liver Animal No: 177 SEX: Female .. ' MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kldney/2 Adrenal Gland/l Spleen Inflanrmatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/96 date of end of treatment: 04/11/98 date of necropsy. : 05/11/96 No findings noted Inflamaaatory cell foci (slight) single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution 1-( No abnormalities found in: Pancreas, KidneY/l, OMry/l, Ovary/2, Adrenal Gland/Z, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT ----I Test Article: TELWER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOs Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 139/146 NQTOX no. : 242933 Propath no.: 99010 Date . : 21.Apr.1999 Animal No: SEX: DOSE GROW: 178 Female PEW MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver ~idney/2 Adrenal Gland/l Spleen Animal No: 179 , . MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : te&al sacrifice necropsy status : planned termFnal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Small glands, n o m 1 morphology. Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, ICidney/l, Wary/l, Ovary/2, Adrenal Gland/Z, Thymus, Mesenteric Lymph Node, Mandibular Lymph N&e/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PATHOLOOP REPORT Test Article: TELOMER ALCOHOL/TELWR ACRYLATE/ :./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services -~ ~ Page 140/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual lhnimal D a t a Records Animal No: SEX: DOSE GROUP: 179 Female PFOS MICROSCOPIC FINDINGS Liver Spleen Animdl No: 180 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen Inflammatory cell foci (slight) Single cell necrosis ( m i n i m a l ) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hemosiderin pipent (moderate) No abnormalities found in: Pancreas, Kidney/l, Kidney/2, oMry/l, Wary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/l, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : termindl sacrifice necropsy status : planned terminal date of start o f treatment: 08/10/98 date o f end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) MidzonaWcentrilobular hypertrophy, diffuse (minimal) Interstitial inflanunation, lymphocytic (dnimal) Hemopoiesis, primarily erythropoiesis (minimdl1 Hemosiderin pigment (slight) No abnomalities found in: Pancreas, KidneY/Z, oMry/l, W a r y / ~ ,Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node& Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT --c1 Test Article: TEL0W.R ALCOHOL/TELOMER ACRYLATE/ `/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 141/146 NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Iadividudl Animal D a t a Records Animal NO: SEX: DOSE GROUP: 181 Female PFOS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen Animal No: 182 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : tennindl sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobulax hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimdl1 Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/Z, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Nodell, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : te&d sacrifice necropsy status : planned terminal date o f start of treatment: 08/10/98 date o f end of treatment: 05/11/98 date o f necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT ---_- Test Article: TELWER UCOHOL/TELWR ACRYLATE/ /Pf'HS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 142/146 NQTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual Animal Data Racords &xhzd No: SEX: DOSE GROUP: 182 Female PFOS MICROSCOPIC FINDINGS Liver Kldney/l Spleen Mandibular Lymph Node/ 1 ANtmdl No: 183 MACROSCOPIC FINDINGS !I. ..... . MICROSCOPIC FINDINGS Liver Kidney/l Spleen Mandibular Lymph Node/ 1 Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) No abnonrtalities found in: Pancreas, ICidney/2, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal G l a n c V P , T h y m s , Mesenteric Lymph Node, Mandibular Lymph Node/Z. days of treatment' : 29 sacrifice group : terminal sacrifice necropsy status : planned tendnal date of start of treatment: 08/10/98 date of end of treatment: 05/11/90 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal /centrilobular hypertrophy, diffuse (moderate) Interstitial inf~anrmation,lymphocytic (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) BACK TO MAIN PATHOLOGY =PORT Test k t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ >/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NPTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a Records Animal No: SEX: DOSE GROUP: 183 Female PFOS MICROSCOPIC FINDING3 (continued) NO abnonualities found in: Pancreas, Kidney/S, Ovary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/l Spleen days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical ( m i n i m a l ) Hemopoiesis, primarily erythropoiesis ( m h h a l ) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, ICidney/2, OVary/l, Ovary/2, Adrenal Gland/l, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lylaph Node/l, Mandibular Lymph Node/Z. BACK TO MAIN PATHOLOGY =RT T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ '/PFHS/PFOS T e s t System : E x p l o r a t o r y 28-Day Gavage Study i n Rats Sponsor : 3~ Toxicology Services Page 144/146 N?TOX no. : 242933 Propath no.: 99010 Date : 21.Apr. 1999 Individual Animal D a t a Records Animdl No: SEX: DOSE GROUP: 185 Female PEW MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 186 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 187 MACROSCOPIC FINDINGS days of treatment : 29 s a c r i f i c e group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 . date of necropsy : 20/11/98 No findings noted Inflammatory c e l l f o c i (minimal) days of treatment : 29 s a c r i f i c e group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory c e l l foci (minimal) MhWmal/centrilobular hypertrophy, diffuse (minimal) days of treatment : 29 s a c r i f i c e group : recovery group necropsy status : planned recovery date of start of .treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 BACK TO MAIN PATHOLOGY REPORT Test Article: TELOHER ALCOHOL/TEL.OMER ACRYLATE/ /PFHS/PE'OS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 145/146 NQTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Individual A n h a l Data Records A n h a 1 No: SEX: DOSE GROUP: 187 Female PFOS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS .. Liver Animal No: 189 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver A n h a l No: 190 MACROSCOPIC FINDINGS I. MICROSCOPIC FINDINGS Liver No findings noted Inflammatory cell foci (slight) Single cell necrosis (nclnimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end o f treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) days of treatment : 29 sacrifice group : recwery gzoup 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 lo findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) BACK TO MAIN Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ .;PFHS/PFOS Test System : Exploratory 28-Day Gavage Study i n Rats Sponsor : 3M Toxicology Services NQTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal D a t a -cords Animal No: SEX: DOSE GROUP: 191 Female PFOS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/90 date of end of treatment: 05/11/98 date of necropsy : 04/12/90 No findings noted Inflannnatory cell foci (minimal) Midzonal./centrilobular hypertrophy, diffuse (minimal1 3M Endronmental Technology. and Services PO Box 33331 - - St. Paul, MN 55133-3331 612 778 6.i;2--" BACK TO MAIN LaboratoryReprt Report of Data for Exploratory 28-Day Oral Toxicity Study in Rats: Telomer Alcohol, Telomer Acrylate, 9 PF'HS, PFOS . Laboratory Report No. FACT-TOX-120.3 Requester Project No. NOTOX #242933 Testing Laboratory 3M Environmental Technology& Safety Services 3M Environmental Laboratory FluorineAnalytical Chemistry Team ( F A 0 2-3E-09 935 Bush Avenue, Ii St Paul, MN 55106 ~~ 1 Laboratory Contact Kris Hansen,PhD. Bldg. 2-3E-09 3 P.O.Box 3331 St.PaMN 55133-3331 Phone: (651) 778-6018 . ;, 1 FAX: (651) 778-6176 Requester Marv Case and Paul Lieder . 3M Company Toxicology Services ' 220-2E-02 St.Paul,MN Rcportissued 6/1Y99 BACK TO MAIN - 3MEiWROhMEN7" L4BORATORY --*_- REPORT NO.FACT-TOX-120 I I I t i I 1 3 1 .l I ! 1 i J 3 1 1 I I I f I i --Le BACK TO MAIN 3MENtlRONUENTALL4BORArnRY .. REPOATNO. FACT-TOX-120 --FACT-M-1.0, ExmctionOfPotassiumPerflsurfactantsdromLivaAnalAFalysisusingHpLc/EsMs FACTM-20, Analysis OfFl~~rochemicianlsLiver- or otherAnionic Fhmmhem'cat Us'ng I K C - E I ~ & O ~ / M ~SpSeSctrometry Samplepreparation-aqueoussamples,HPLCDBMSWk ion-pairing ertraction '. Analyte is atmctedhrna sample matrixwithionpairing reagent [tembutyl ammonhmhydrogend k t e ("BA)] mapHantrolledenviroment Thecation.icreageatselectively.targetsanionicfluorochanicals Once the anion-TBA pair is fo- the analyte is transferred into a non-polar Organic solvent (methyl-tetfbutylether)dried,andandreconstautedinme~lforhGanalysis. HPL- andHPLc/EsMsMs:ordetailed qualitativework , InHPLc, analiquotdexmctisinjectedand passedthroughareversephase liquidchromatograpl.6c column Based onthe ofthe a d y k forthestatio- phaseinthe columnrelativetotheliquid mobile phase, the amlyte is retainedfora chamcm* m'camountdtime. Forexample,inastandard e solutionPFOS may eluteat 5.0 minutes. Retention timesbetweena stanrlardPFOSsolutionandthe analyte extmedbmliverinthis analyskwerematchedontheHPLC system towithin 1% .. c FollowingHPLC @on, ESIvfSMSprovidesarapidand accurattmeansform a w i d e of 0rganiccoTnpoundSincludingfhxo~caIsE. lectrospray,aniionizatontechni~usedprimarityfot - detection ofmolecularions, isgenerally operatedatrelativelymildtanperirhaes. Molecules areionize4 andaprimary ion,dmckmt'c~dtheanahrte.is&ected. T b i s i o n i s b o m b a r d e d w i t h l l i ~ ~ g a s ; nhquent collisionscfeated e r secondary ionichgmentsunique toh te mion, whichare ddccted :\ For examplqforPFOS (C&,%') adysis, ion499 isselected asthe cbaracten'st*tcpp.imaryion. Thision iskgkntedinto other ions suchas 80 amu(comqondingt0 SQ> and230amu(W&QJE.ach& thcstseconQIyh~isdetectedandonbeusedtoditrerentiatePFOS~motherco~thaX migIlthavtthesamecharacten*sh'c 499 amuprimaryion, but differentchemical compositionsand sccondaryionhgmentationpatterns. --..-- BACK TO MAIN 3hfE"MpIITRL LABORATORY REPORTNO. FACT-TOX-120 Quantitationofthct a r g e t e isbased onlinearregresionadysis(weighted l/x, ifappropriate) af t w o ~ m a t r i x c u r v e s b r a c k e t h g e a c h p p o f s a m p l e s .@aufbtionofeachanalyteisbasedinthe responseofoneormore specific daughterions usingthemultiplemqonse-monitoringmode dthe 5.2 Blanks ~~Onb~~waterassunogatematrbSare~witheachbatchd~. AdditionallyDtwosamples ofblankmatrix, usedfor the exhcted&%ration c t m q axeexmcted along witheachbatchafsamplesandcunrts. Blaoks arccompliantifno target- isdeteckdabovethelimitddetedionforaspedlcamlyre. In thisstudy,allblankswere&mpliant . 5.3 Surrogates Tetra-hydro panuorooctanc sutfonate is used as a smogate in this adysis. Sumgate resganse is monitondtoconfirmgrossinstnrmentalmmif-. 5.4 Matrix Spikes Duplicate matrix spike analysesm a ltarget aralytes were prepxed and adyzed for one amtrol animaL Except as noted in the resultstable,recoverieS w ~ r ewithinthe acceptable range af7O-l3O??a Recoveriesoutsidethisrsmgtmaybe~uated. 5.5' Laboratory Control Samples Laboratorycontrol sample^ arenot acamponentdthisstudy. - --*_e BACK TO MAIN 3.MEWIRONMENTALIABORATORY REPORTNO. FACT-TOX-I20 .. 9.1 AttachmentA: Table&Results of Analysis of Liver Samples 9.2 AttachmentB: Tableof FACT acronyms and formulas 9.3 AttachmentC GraphicalResultsby Groupand Gender 9.4 AttaehmentD~ographicresultsofpFcAinvestigidionin~2andOraap3 9 3 AttacamentECbaindCustody 9.6 AttachmentF:Analytical Data 1 I I i 1 ;' 1' 1 1 I : i i 1 i ! i i' 3M Envi'nrnenntal Labomtvm - F1uod.eAnahti& ChemisW Team f m m FACT-TOX-120, Rat Livers 28-DayOral ToxStudyby DajIyGavage in Rats BACK TO MAIN .. I I 377+/-31 I 240+/-25 I 191+/- 15 I I I I I I 66 419+/-86 309+/-34 237+/-25 . '. I (Faale) I G1 G5 + (Male) G1 G5 PFHS (CdF 13SO3.) I' Day 1 Day 14 Day28 I I <MDL 97.0+/- 14.7 <MDL 0.26+/-0.12 I I <MDL 0.03 +/-0.01 I I I Day 1 0.055 +/- 0.020 327+/- 52 Day 14 0.129 +/- 0.088 61.9 +/- 11.7 Day28 0.089 +/- 0.019 36.3 +/-7.4 .?7..... . . . G4 I 0.37+/- 0.38 I 0.02+/-0.00 I <MDL I 64 I 3.09 +/- 1.58 I 0.126 +/- 0.084 I 0.025 +/- 0.015 I 3M Confidehtial 3M Envimnmentaf Labmatow - iffforheAna/vticafChemistmTeam //Am FAc;T-TOX-l2~Rat Livers 28-Day Oral ToxStudy by Daily Gavagein Rats G23 .I0.112+/-0.039 44DL G6 23 I 6.59 +/- 2.16 8.64 +/- 1.48 I 3.59 +/- 0.67 3.36 +/- 1.65 I 1.76 +/- 0.23 1.26 +/- 0.44 I M513 (C9F19CO2-) -O F d e ) G1 I I 1. G23 . I - (Male) G1 I I I I G32 .. Day1 <MDL 1.69+/- 0.18 1.20+/-0.30 Day 1 <MDL 1.91 +/- 0.88 1.78 +/- 0.3 1 Day 14 Day28 <MDL <MDL 1.36+/-0.02 2.24+/-0.22 1.20+/-0.09 0.812+/-0.188 . Day 14 <MDL 1.73 +/- 0.46 1.08 +/- 0.26 Day28 <MDL 1.52 +/- 0.32 0.801 +/- 0.148 G3. 0.117 0.029 +/+/- 0.020 0.017 I 0.085 0.035 +/+/- 0.019 KO15 I 0.101 0.036 +/+/- 0.012 0.01 1 BACK TO MAIN ** C9,C11 and C13 perfluorinatedcarboxylicacids also detectedin G2 and G3 animals 3MCOnfidential . Frn-M-20 M vmia 7.a BACK TO MAIN I BACK TO MAIN ,:. -- i.-b-.- * .-.-.- *"- w' L4.- -a F A C O G 1 2 0 L - -4 , =Day Oral T OS~h d yby Dally GavageIm R.0 BACK TO MAIN am QQ*. am Q(DL 0.m Qw. om QmL am am. 17.1 am Qw. BACK TO MAIN rAclJCz0 L.rrlV& 7.0 . .-... .. BACK TO MAIN I c4md +iaio-I - W6 49.7 lo.9 31.3 I I %I ma aun 41.9 ))rl 0.6344 445 IOU awn 2ll aMl9 oM4l BACK TO MAIN BtI ld mt BACK TO MAIN nt ee ede are aTe er Te [oo] BACK TO MAIN rACr.M-2.0 ErrdV A 7.0 BACK TO MAIN I 3M Environmental Lab - Fluorine - AnalyticalChemktry Team ._. I PFS's i 1 PFHS pduorohexanesulfonate C6F13S03- I PFOS peffluorooctanesulfdnate PFOSA PFOSAA C8F17S03C8F17S02NH2 C8F17SO2N((CH2CIu)(CDCOO))- ' 1 M556 C8F 17S02N((H)(CH2COO))- M570 C8F 17S02N((CIu)(CH2COO))- PFOSEA peffluorooctanesulfonylethylamide CSF17SOZN((CH2CH3)(H)) ' 1 . Et-FOSE-OH PFDS perfluorodecanesulfonate C8F17S02N((CH2CH3)(CH2CH20H)) C1OF21SO3-** .. I PFCA's 0 POAA perfluorooctanoate c7F15coo- .. 1, M513 M613 M463 perfluorodecanoate pduorododecanoate i M563 M363 ' C9F19CQOC11F23COO- C8F17COO- ** ClOF21COO- ** C6F13COO- ** I PFS = perfluorinated sulfoante I PFCA = perfluorinated carboxylic acid 1 I ** no standardmaterial available for confirmation; '....**: 1- . 1 I f I I 1 I i i. - - 3M Confidential Attorney/Client Work Product Privileged Do Not Disclose BACK TO MAIN 1 - --?*_- I - 0.8 I I 0.7 -- 11I 4 18s h I . in Male and Female Rat Liver, Group 4 3M EnvironmentalLab FluorineAnalytical Chemistry Team, Project: FACT-10x120 +Female 1I 5 --t Male 4.5 33*5 S 2.5 2 8" 1.5 3 1= 3 '0 1 0.5 0 28 1 PFHS in Male and Female Rat Liver, Group 5 - 3M EnvironmentalLab Fluorine-Analytical Chemistry 1' 120 Team, Project: FACT-10x120 ,400 I 1. 1 +Female +Male 350 i -.300 8C I .T ' 250 '200 P 08 1 P 40-- 1 1 14 28 3 bY . PFOS in Male and Female Rat Liver, G'hup6 . - 3M EnvironmentalLab FluorineAnalytical Chemistry 1 . 450 Team, Pdect: FACT-10x120 - 600 -500 II --f!- 300 8 8" 150 i Q) 50 -L - 200 3 - 100 4 1 1I O! I I I !O 1 14 28 Day BACK TO MAIN 1 1 - 0.25 r 1 I z o*2:: I 89 1 5 p 8' "B 0.1 -- 2 1 0.05 -L 3 0 POAA in Male and Female Rat Liver, Group 2 10 1-D- Female --tMale 1 - - 9 s "-7 --6 ZLs --5 BE --4 - - 3 s! --2 2 -- 1 0 1 14 28 Day '1 M513 in Male and Female Rat Liver,.Group 2 - 3M EnvlronmentalLab FluorineAnalytical Chemistry Team, Project FACT-TOXl20 * 1 3 3 T 1 5 2.5 % 2 I Bin.1.5 as I & 0.5 1 0 1 28 . 3 :\ I - 0.16 I . M613 in Male and Female Rat Liver, Group 2 3M EnvironmentalLab FluorineAnalytical Chemistry Team, Project FACT-TOX120 I 0.16 1 0.14 0.14 I - I ! i 0.04 Q) 0.04 1 lb 0.02 0 0.02 , 1-m- Female +-Male 0 I I I 1 14 28 Day . 1 'OX-12O919GM2 60899007 1 I % I 1 3 'I 1' 1 I "/o-: .I b3 f %3 1 I %- t i J 1 I . --LP 5.98 I6.10 BACK TO MAIN 7: MRM of 1 Channel ES- . 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' ' ' ' 536 I 8.68e: r- TIC 6.65e: | '0X-120; 113F-G2, liver | |030299003 I= | ods 120180230 = 33 1030299003 I ] ] ] 030299003 1| | ' BACK TO MAIN soup 020199 02-Mar-1999 10:28:00 921 6: MRMof re 2ChanneElSs- - 1.673| EIEBCo0) 303855 Sen 138 as|| bd 1045 nus) 11S.68 220w ass 5: MRMof 2 ChanneElSsTIC| 5.23e4| . 828 . 4: MRM of 2 Channels ES- TIC| 337e4| | 0130299003 ] 3: MRM of 2 Channels ES- 7.68 TIC| 1.054 i 1 4 030299003 1 ` I, | J1030299003 | % sping | 66 2.00 6% " TIC] 2: MRMof 2 Channels ES- . 3.63e3| na 1: MRMof2 Channels ES- a Er591 wos wm ue ; 541 634 so\ben ar8|70 9% 10.56 1116 1256 '1336 Time| 4.00 6.00 8.00 10.00 12.00 BACK TO MAIN 100- 9& pmmiq i %- piFim5.I .-\ %: pm3mq _ _ 2.60 1.24e4 785 -? 3.89e3 ET, Tm me en a le BACK o TO MAIN o SMReuter 7 dr Wiaso [75-77 Ti SEiomeorTa aborE tBE EBi AOSRC EAE R AR | eno] n| Baa, + [Tare sue = dade ftSLi ET A od KIH|_ogladna a:, SFomRecuEsesTin | riheEAREAEotEa nmmm MaSa aea e aCosn aefe Os |wo|| Ee eee ri mre ES PSR He ee ooSt Shae e E et a e R Ts nlC ieE ee ee | | : bows:[ome nelCaen fry en {ened mnen) fEmeseLos Ea r PLoo n ke eeee| {f e fr e = froereboombehao n eenea eeeeee[ow [oe " BeemsenTtotoeh loo l nb[oeens Poainmeen| eonfoe Frehake cess madmanTeeese BACK TO MAIN n &%~iedNunb.r . . ... . _. ... I I Item # I Relinquished By/Affiliation I Time I Date I ShippedVia I Received By/Affiliation , I I . Time . :I. ~~~~ Date .. I I SppaiingofCustody Hacora BACK TO MAIN 09718" [CC T Hkoemo apa ]| eee Ne [PT om, owrm wa 34183[a]| |32 Con Cobb snscl Ele TT ee [TTT Tae|Te dn] Flops TT Tie [TT Tag]-- 0 Coles TeTITTT as| jog] Clee 1 TeTIT lag| Cole TTTe TTI Ila]-- CC ol TT T re o ee[rIIrTrIrT ag --r -------------------------- 7 I [Ee| folTPT ee fel ee fe TTrrr Lol -- OF FEE] tems |RolinquishedyiAftilsion | Time | Date | ShippedVia | ReceivedByAiiston | Time | bate | [ Ilef Te rr rm gene CT tr h TT h TrebT g da TT T ] NOTOX B.V. BACK TO MAIN .. 1 .. PROTOCOL 1' EXPLORATORY 28-DAY ORAL TOXICITY STUDY i. WITH . TELOMER ALCOHOL, TELObER ACRYLATE, PFHS AND PFOS (positive control) BY DAILY GAVAGE 1 IN THE RAT FOLLOWED BY A 14/28-DAYRECOVERY PERIOD t I 3 ?. '4. 11 NOTOX Project.242993 .% . . ., NOTOX Substancrr 8 3 a 4 , 83499, 83S02, 844S6 and 84141' 3MTmrt No. T-7023. T-7024. T-7022. T-7032,T-6293 .I - page 1 o f 16 - 11/09 '98 1 6 : 2 4 FAX 31730118543 7. I 11 TELOMEF? ALCOHOLITELOMER ACRYLATE!' NOTOX B.V. -. ---& IPFHSIPFos PROTOCOL APPROVAL BACK TO MAIN NOTOX Project 242933 3W Test No. T 7022 T-7023, T-7032, -T-7024 T 6295 3 STUDY DIRECTOR : 'I Ir. S.C.M. de Hoog '-. I....,.. Date: 71 sQp&m-& oa 'g38 SPONSOR : J - j 1 Marvin T. Case, DVM, PhD . Corporate Scientist 3M Toxieo1ogy Services aa3aM-2 . .......- T L m.............. . i '? ' Cr ' 11/09 ' 9 9 16:25 FAX31736418343 1 1 NOTOX B .V. ---- TELOMER ALCOHOLlTELOMER ACRYLATE/ 'IPFHSIPFOS BACK TO MAIN .. NOTOX Project 242933 PREFACE Sponsor 9M Toxicology Services 3H Center, Bldg 220-2E-02 S t . Paul. MN J5144-1000 USA Study Monitor Marvin T. Case, DVM, PhD 3M ToXIcology Services 3M Center, Bldg 220-2E-02 Telephone (651)- 733-5180 Fax (651)- 733-1779 Involee Contact . Testing Facllity Barbara Nelson 3M Procurement Operations 3M Center, Bldg 224-1N-04 St. Paul. Mu 55144-1000 Telephone ( 6 5 1 ) - 733-0766 Fpx . (651)- 733-1799 . NOTOX B V. Hsmbakcnwetering S S a l DD 's-Hertogenbosch The Netherlands f Study Director It-. S.C.M. de Hoog Pathology Or. J. Th. Wilson .-\ - Page 3 - I BACK TO MAIN 1 1 TELOMER ALCOHOllTELOMER ACRYLATE/ ' /PFHSlPFOS NOTOX Project 242933 1 STUDY PLAN 1 Start o f Treatment 3 06 October 1998 (males)' 08 October 1998 (females) S t a r t Recovery 1 04 November 1998 (males) 06 November 1998 (females) Blood sampling 1 03 November 1996 (Maln groups, males) 04 November 1998 (Main rnd Recovery groups 1and 2, males) 05 November 1996 (Main groups, females) 06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1, males) I . 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females) I Uri& c o l l e c t i o n predose day 7 1 day 7 day 14 ' day 14 end OF treatment I end,oftreatment end of r e c o v e r y end o f recovery end of recovery 3 end o f recovery : 29-30 September 1998 : 12-12 October 1998 : 13-14 October 1998 : 18-19 October 1998 : 20-21 October 1998 : 03-04 November 1998 : 05-06 November 1998 : 17-18 November 1998 : 19-20 November 1998 : 01-02 Dacrmber 1998 : 09-04 December 1998 :Recovery [Recovery [Recovery :Recovery :Recovery :Recovery :Recovery :Recovery :Recovery :Recovery :Recovery groups groups groups groups groups groups groups groups groups groups groLps 1 and 2) 1 and 2, males) 1 and 2, females) 1 and 2, males) 1 and 2, females) 1 8 n d 2, males) 1 and 2, females) 1, males) 1, fema7es) 2, males) 2, females) . Necropsy 3 03 November 1998 (Main groups, males) 04 November 1998 (Main groups, males) 05 November 1998 (Hain groups, females) I 06 November 1998 (Main groups, females) . 18 November 1998 (Recovery groups 1, males) .20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) I 04 December 1998 (Recovery groups 2, females) 1 Draft Report 26 March 1999 I Page 4 - i NOTOX B.V. BACK TO MAIN @ 007/017 1 TELOMER ALCOHOLITELOIOR ACRYLATEI IPFHSIPFOS 1 NOTOX Project 242933 .i TEST SUBSTANCES I The sponsor is responsible for the completeness Of all test substance data. All available information on characteristics of the test substance will be taken into account before start-ofthe study in order to determine .If they conflict 3 withhlght affect the proceeding o f the study. NOTOX SUBSTANCE 83464 (data will be added by protocol amendment) Identitlcation Description -1 Batch Purity Test substance storage . Stability under storage i conditions Expiry date TELOlk(ER ALCOHOL (T-7023) Stable 1 Stability in vehicle 1- Safety precautions 1% aqueous carboxymethy1.cellulose: not indicated Corn oil: not indicated Gloves, goggles and face mask to ensure personnel health and safety I Disposal Category i NOTOX SUBSTANCE 83499 (data will be added by protocol amendment) I Identification TELOMER ACRYLATE (T-7024) Description a Batch _I Purity Test substance storage Stability under storage conditions Stable Expiry date Stability in vehicle 3 Safety precautions 1 Disposal Category . 1% aqueous carboxymethyl cellulose: not lndicated Corn 011: not fndicated .-\ Gloves, goggler and face mask to ensure personnel health and safety . i .1 - Page 5 - ! BACK TO MAIN 11 TELOMER ALCOHOLITELOMER ACRYLATE/` lPFHSIPFOS .. NOTOX Project 242933 I NOTOX SUBSTANCE 83502 (data will be added by protocol amendment) I Identification Descrlptlon Batch (T-7022) 3 Pur1ty Test substance storage Stabtlity under storage conditions Stable 1 Expiry date Stability in vehicle Safety precautions 1% aqueous carboxymethyl cellulose: not indtcated Corn 041: not indic8ted Gloves, gobgles and face mask to ensure personnel 1 Disposal Category health and safety NOTOX'SUBSTANCE 81456 (data will be added by protocol amendment) Identification l- Oescription . . Batch Purity Test substance storage 1 Stabtllty under storage conditions Expiry date Stability in vehicle . 1 Safety precautions 1 01sposal Category PFHS (T-7032) Stab 1 e 1% aqueous carboxymethyl cellulose: not indicated. : Corn oil: n o t indicated * Qloves, goggles and face mask to ensure personnel health and safety 1 NOTOX SUBSTANCE 84141 (data wlll be added by protocol amendment) Identification PFOS (T-6295) .-\ Description Batch Purity 3 Test substance storage Stablllty under storage conditjons Stable Expiry date 1 Stability in vehicle Safety precautions 1% aqueous carboxyamthy1 cellulose: not indicated Corn o i l : not Indqcated Gloves, goggles and face mask to ensure petSOnne1 I` I Disposal Category health and safety Vehicle I Rattonale f o r vehicle I - - Stabllity in vehicle 1% aqueous carboxymethyl cellulose o r Corn 011, specific gravity 0.92 Based on trial formulations performed at NOTOX I Stability over 4 hours will be determined by t h e sponsor Page 6 i BACK TO MAIN TELOMER ALCOHOLlTELOMER ACRYLATE/ lPFHS/PFOS NOTOX Project 242933 TEST SUBSTANCE FORMULATION . Method Storage conditlons Formulations (w/w) will be prepared daily within 4 hours prior to dosing. If necessary, adjustment * . will be made f o r specific gravity o f vehicle. At ambient temperature No Chemlc81 analysis o f dose preparations wlll be performed. PURPOSE AND RATIONALE The nature and purpose of this exploratory study wlll be to determine and compare the relative oral toxicity i n rats following oral (gastric gavage) doses administered daily over 20 days and identify developmental compounds o f lesser toxiclty f o r commercial development. This study should provide part o f a ratlonal basis f o r toxicologica'l risk assessment I n man. The oral route was selected as it is a possible route o f human exposure during manufacture, handling or use o f the test substance. WIDEL INES i Thls protocol was reviewed and agreed by the Article 14-functionary agd.the Ethical Comittee o f NOTOX as required by the Dutch A c t on Animal Experimentation. The study procedures deacrlbed +n this protocol will be based on the following guidelines: 1) EC Directive 96/!54/EEC, Annex XV.D, replacing EEC 01-rective67/548IEEC, Part 8 : Methods for the Determination of Toxicity and othar'Health Effects; 8.7: 'Repeated Dose (28 days) Toxlcity ( o r a l ) * . Official Journal o f the European Communities .No. L248. September 1996. 2) OEW Guideline- for Testing o f Chemicals, Guideline 407: 'Repeated Dose 28-day Oral Toxicity Study in Rodents., Paris Cedcx, 27 July 1995 ' This p r o t o c o l , study procedures and the report will not be fnspected by the . . NOTOX Quallty Assurance Unit. ARCHIVING NOTOX B.V., will archive the following data for at least 10 years: protocol. raw data, report and a l l specimens. No data will be withdrawn without the sponsor's wrltten consent. - Page 7 9 NOTOX B.V. BACK TO MAIN 010/017 TELOMER ALCOHOLITELOMER ACRYLATE/ IPFHSIPFOS NOTOX Project 242933 TEST SYSTEM lest System * Age at Start of Treatment Number o f animals Randomisation Identification Sprague Dawley rat Crl:CD 8R, outbred, SPF quality. Recognised by international guidelines as the retonmended test system (0.9. EPA, FDA, OECD, EEC). Females wlll be nulliparous and non-pregnant. Source : Charles River, Sulzfcld, Germany Approximately 6 weeks 84 males. 84 females A t least 5 days bOfOr8 study start, by computergenerated random algorithm according to body w e Q h t , with all animals within i20% of the sex man. A health lnspectqon will be performed prior to cornencement o f treatment to ensure that t h e animals are in a good-state o f health. Earmark and tattoo ALLOCATION Group Identiticatjon Test Substance Number o f animals males females 1 Main group Vehlcla control 8 8 1 Recovery group I 1 Recovery group 2 2 Maln group Vehqcle control Vehicla control Telomcrr Alcohol 3 3 3 3 8 0 2 Recovery group 1 Telomar Alcohol 3 3 2 Recovery group 2 3 Main group 3 Recovery group 1 Telomer Alcohol Telomer Acrylate Telomer Acrylate 3 3 8 :a 3 3 3 Recovery.group 2 Telomer Acrylate 4 Main group 3 3 0 0 4 Recovery group 1 3 3 4 Recovery group 2 3 3 S Main group PFHS 8 0 5 Recbvery group 1 PFHS. 9 3 I S Recovery group 2 PFHS 3. 3 6 Main group PFOS (posltlve control) 8 8 6 Recovery group I PFOS (posltive control) 3 9 6 Recovery group 2 PFOS (positive control) 3 9 Animal numbers males females 1- 8'' 9-11 13-15 17-24 25-27 29-31 33-40 41-43 45-47 49-56 57-59 61-63 65-72 73-75 77-79 81-88 89-91 93-95 97-104 105-107 209-111 113-120 121-123 125-127 129-136 137-139 141-143 145-152 153-155 157-159 . 161-168 169-171 173-17s 177-184 185-187 189-191 Test substances Telomer Alcohol (substance 83484), tclomer Acrylate (substance 83493) and (substance 83502) to be tested at a dose level o f 30 mg/kg body wetghtlday, - PFHS (substance 84456) to be tested at a dose level of 10 mg/kg body weightlday. PFOS (substance 84141) t o be tested at a dose level of 9 mglkg body woight/day. Page 0 = 11/09 'B8 16:27' FAT 31736418543 I i NOTOX B . V . ---_- BACK TO MAIN 11 TELOMER ALCOHOIITELOMER ACRYLATE1 lPFHSlPFOS NOTOX Project 242933 1 ANIMAL HUSBANDRY Conditions 1 Air-conditioned room with approximately 15 air changes per hour and controlled envlronment with opttmal Conditions being a temperature o f 21 f 3.C and a relative humidlty of 30-70%. Lighting Will be f 12 hours artlficlal fluorescent 1 llght m d t 12 hours dark per day. Accommodation Group housing of 3-4 anlmalo per sex per cage In stain'less steel suspended cages - wtirtehatwmiernte munedsehrflloaobrosr.atAocrcylicmoantdiistaiotnlso.n period I s at least 5 days before start o f 1' Diet free access to standard pclleted laboratory anlmal diet (from Carfil Quality BVBA,.Oud-Turnhout, Betglum). Each batch will be analysed for nutrlents and I . contaminants are analysed on a regular basis. Results are examined and archived. Water free access to tap-water. Certiflcates of analysis (performed quarterly) are examined and archived. I i TREATMENT I 1 Method 1 Frequency 1 Dose volume I Dose level I i Oral gavage, using a stainless steel stomach tubs. Formulations will be placed on a rnagnetic+stirrer ' I during dosing. " . Once daily f o r at least 28 days. approximately the same time each day, 7 days per week. Maln group animals are dosed up to the day pr3or to necropsy. 5 ml/kg body weight. Actual dose volumes will be calculated weekly according to the latest body wefght. Group 1: 0 mg/kg b.w.lday Group 2: 30 mglkg b.w./day Group 3: 30 mglkg b.w.lday Group 4: 30 mglkg b.w./day Group 5 : 10 mglkg b.w./day Group 6: 3 mglkg b.w.lday (Vehicle Control) (Telomer Alcohol) (Telomer Acrylate) (PFHS) (PFOS, porttive control) i 1 RECOVERY i Duration i Recovery group 1: at least 14 days Recovery group 2: at least 28 days t 1 1. - Page 9 - BACK TO MAIN 11 TELOMER ALCOHOLKELOMER ACRYLATEI: IPFHSIPFOS .. NOTOX Project 242933 1 OBSERVATIONS 1 Mortality / Vlability Twice daily. Anlmals showing pain, dtstress o r discomfort, which is considered not translent i n . nature o r is likely to become more severe, will be 3 Clinlcal signs 1 1 Body mlelghts sacriflced for humane reasons. The time of death -will be recorded as precisely as possible. Twice dally, detailed cllnical observatlons w i l l be made i n all anlmals: once Immediately after dosing and once approximately 4 hours after dosing. The time o f Onset, degree and duration will be recorded. All symptoms wlll be recorded and graded according to fixed scales (specified in the report). treatment period : on days 1, 5, 8, 12, 15, 19. 22, I. 25 and 28. Recovery period 25 and 20. : on days 1, 5. 8, 12, 14, 19, 22, Food consumptian .1I Water consumption Weekly. Subjective appraisal will be maintained durlng the study and a quantitative assessment Introduced I n the event o f a suspected treatment related effect. t CLINICAL LABORATORY INVESTIGATIONS (Main and Recovery group animals) -" J Blood samples will be collected under light ether anaesthesia, between 7.30 and 9.30 a.m. 1auPediately prior to post mortem axaminatlon f o r Main and Recovery group animals, and for Recovery group animals also at the end o f the treatment period. The animals will be f a s t e d overnight (with a maximum of 20 hours) before , blood sampling. but water wlll be provided. Blood samples will be drawn from the 1 retro-orbital sinus o f all Main and Recovery ratslsex/group and collected into tubes prepared with EDTA f o r haematologlcal parameters (0.6 ml), with citrate f o r clotting tests (1.0 ml) and untreated tubes for clinical biochemistry parameters (>2.0 ml). I 1 i I I 1.2 1 1 - Page 10 - BACK TO MAIN 1I. TELOMER ALCOHOLlTELOMER ACRYLATE/ PFHSIPFOS . NOTOX Project 242933 HAEMATOLOOY The f o l l o w i n g haematology parameters will be determined from b l o o d prepared w i t h EDTA as an anti-coagulant, using the Instruments .listed: Parameter Urilt Instrumentation Erythrocyte count T I1 Sysmex K-1000 Haemoglobin nrao1/1 Syrmex K-lo00 Haematocrit 111 * Sysmex K-1000 Maan corpuscular fl vo 1uma SYS-X K- 1000. Mean corpuscular haemoglobin . - Mean corpuscular haernog.lobin concen trat Ion fmol mmOlI1 Sysmex K-1000 SySmX K-1000 plate let ' count GI 1 Sysmex K-1000 Red cell d i s t r i b u t i o n width %, Sysmex K-1000 Total leucocyte count W 1 Sysmex K-1000 :2 .'? . Differentlal leucocyte l(re1) count Manual (Mlcroscopc) The following haematology.parameters w l l l be datarmined.fron blood prepared with cltrato as en anti-coagulant. using the Instruments llotbd: Parameter . Unit Inttrumantatlon Prothrombln time tec J1 * Ptairmteial thromboplastin tec Sysmex CA-5000 Sysmex CA-5000 - Page I1 - BACK TO MAIN 1 TELOMER ALCOHOLITELMR ACRYLATE/' MTOX Project 242933 1 CLINICAL BIOCHEMISTRY 1 The following clinical biochemistry parameters will be determined f r o m serum samples usfng the Instruments listed: Parameter Unit . Instrumentation 3 Alanine aminotransferase . pkat/l Epos selective analyser 5060 (Eppendorf) Aspartate aminotrans- pkrt/l farase Epos selective analyrer 5060 (Eppendorf') Billrubln, total )uno111 Epos selective analyser 5060 (Eppendorf') Cholesterol, total molJ 1 . triglycerides molt1 t . Creatinlne . mol / 1 Glucose m o l 11 Epos selectlve analyser 5060 (Eppendorf) Epos selectlve analyser SO60 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060' (Eppendorf) Urea . mol11 Epos selective analyser 5060 (Eppendorf) Protein, total . 011 Epos selective analyser 5060 (Eppendorf) Protein, albumin g/l J Protein, globulin 01 1 Albumin Globulin r a t i o 1 Alkallne phosphatase Mat11 - Epos selectlve analyser 5060 (Eppendorf) c Calculatlon [Proteln total Aibumln] Calculation fAlbumin/blobulin] Epos selective analyser 9060 (Eppendorf) Sodium If Potassiurn Chloride Cal c 1urn mnolll molI 1 mmolll moll1 EFIX 5045 Flame.-P\hotometer (Eppendorf) EFIX 5055 Flame Photometer (Eppendorf) Jenway P C L W EFIX 5055 Flame Photometer (Eppendorf) Phosphorus mo1/1 Epos selective analyser 5060 (Eppendorf) - Page 12 - BACK TO MAIN 11 TELOMER ALCOHOLllELOMER ACRYLATEI' IPFHSIPFOS NOTOX Project 242933 1 URINALYSIS (Recovery group animals Only) Urine samples will bo collected overnight (approximately 16 hrs) P r o m all 7 animals ssslgned to the Recovery groups according t o the following schedule: - predose 3 - during the treatment period on days 7, 14 and at the end o f treatment - at t h e end of the recovery period, prior to scheduled necropsy. '.I During the sampling period animals will be deprived o f food but vater will be provided. Urine will be collected into a rpeclaoan vial, using a metabolism cage, After collection the samples will be frozen and stored at -20% at NOTOX until further analysis. 1 . . PATHOLOGY I I - NECROPSY I All animals surviving to the end of' the observation period and all moribund animals will be deeply anaesthetired using ether vapour and subsequently exsanguinated. All animals will be necropsied and descriptions o f all macroscopk abnormalities will be recorded. 1 Prior to exsanguination a blood sample (1-2ml) f o r serum collection will be taken from the aorta. Serum samples will be stared at -20% at NOTOX until further analysis. C 1 Samples o f the l"allow1ng tissues and organs will be collected from al$animals a t necropsy and fixed ln neutral phwphate buffered 4% formaldehyde solution: Identification markg 1"1 pdrenal alands ' Aorta ' Brain Caecum .+\ 1. Colon Duodenum ' Epldldymider Eyes with optic nerve and Harderlan gland 1 Female manunary gland area Heart I1 eum Jejunum Kidnevt Liver I - 1 Luna. infused with formalin 9 I Oesophagus Ovaries- ! .- Pancreas I I - Page 13 - BACK TO MAIN TELOMER ALCOHOLITELOMER ACRYLATE/ lPFHSlPFOS Noiox Project 242933 Peyer's patches (jejunum, ileum) if Pituitary gland - Prostate gland Rectum Sallvary glands Sciatfc nerve mandibular Seminal vesicles Spinal cord -cervical. midthoratlc, Soleen Sternum w f t h bone marrow Stomach Testes Thvmus Thyroid including parathyrotd . Tcachea Urinary bladder Uterus. All gross leslons . detectable lumbar . ORQAN WEIGHTS The followlng organ weights (and terminal body weight) will be recorded from the surviving animals on the scheduled day o f necropsy: Adrenal Brain Heart Kidneys Liver Ovar its Spleen Testes glands Both absolute and relrtlve (% body weight) organ weight data wlll be included in t h e study report. .-\ LIVER SWLTNG From a11 animals surviving until the scheduled necropsy at least 1 gram of the left lateral liver lobe ut11 be frozen in ioopentanelN1. Llver samples wlll be stored at -20% a t NOTOX until further analysis. - Page 14 - 11/09 ' 9 8 16:30 FAX 31136118543 NOTOX B .V. 1 -- - . BACK TO MAIN @017/017 -_ . I 3 TELOMER ALCOHOLITELOMER ACRYLATE/ PFHSlPfOS NOTOX Project 242933 I 1 HISTOTECHNOLOGY All organ and tissue samples, as defined under Histopathology (following), will 3 be processed, embedded and cut at a thickness of 2-4micrometers and stained with haematoxylin and eosin. Special stains will be used at the discretion o f the pathologist. '1 HISTOPATHOLOOY 1 Slldes o f all underlined organs and tissues .(see under Necropsy) collected at the scheduled sacrifice from all animals, as well asafrorn all animals o f all dose groups which died spontaneously or were terminated In extremis, and all gross lesions o f all animals (all dose groups) will be examined by a t pathologist. All abnormalities will be described and included in the report. I- I I I 1 3- 1 *I 1 i f ! STATISTICAL ANALYSIS The following statistical methods will be used to analyse the data: If the variables can be assumed t o follow a normal distribution, the Dunnettt e s t (many-to-one t-test) based on a pooled variance estimate will be applied for the comparison o f the treated groups and the control groups f o r each sex. The SteeLtest (many-to-onerank test) will be applied instead o f the Dunnett- test ifthe data can not be assumed t o follow a normal distrtbution. All t e s t s will be two-sided and i n all cases p < 0.05 wiql be accepted as the lowest level of significance. Additional methods o f statistical analysis may be used at the discretion of the Study Director. The methods and the results will be described in the report. References : - C.W. Dunnett A Multiple Comparison Procedure for Comparing Several Treatments with a Control, J . h e r . Stat. Assoc. 50, 10964121 ( 1 9 5 5 ) . - R.G. Miller Simultaneous Statistical Inference, Springer Verlag, New York (1981). ! BACK TO MAIN 1 . A Page 15 - 1 EtOMER ALCQHOLITELOMER ACRYLATE/ IPFHSIPFOS I DISTRIBUTION This protocol will'be djstributed as follows: Orlglnal : . 1 copy: 1 copy: 1 copy: 1 copy: 3 copy: 1 copy: Study Director Coordinating Blotechn1cian Clinical Pathology NecropsyI H Istotechnique Histopathology Management Sponsor NOtOX.Project 242933 9. :. . . I . 1: 1. 1 1 I 3 'I 1' I 1 I I T . {' .. ...-. f: , ....... ... .. 3 I / 1 1' *. t" I f BACK TO MAIN STF HIS 325/03 -*.._- Safety & Environmental Research Rv, SPECIMEN DISPATCH L I S T .. NOTOX PROJECT: -242933 SPECIES : -RAT TEST SUBSTANCE: 83484, 83494, . 83502, 84456, 84141 SIGNATURE/DATE STUDY DIRECTOR: r TO: Name and adress company: 3 M Environmental Technology and Safety Services Attn. K r i s Hansen 935 Bush Avehue B u i l d i n g 2-3E-09 Group 1 Group 2 -. . Group 3 Group 4 Group 5 Group 6 Animal numbers males 1-li+ 13-15 . 17-27 + 33-43 + 49-59 + 65-75 + 29-31 45-47 61-63 77-79 81-91+ 93-95 Ani mal numbers femal es 97-107 + 113-123+ l29-l39+ 145-155+ 161-171+ 177-187 + 109-111 125-127 141-143 157-159 173-175 189-191 Number o f l i v e r samples 28 28 28 28 28 28 i Also enclosed are : [ 3 Copy of Protocol/Amendments [ 3 Copies of h i s t o l o g y raw data [ 3 Study results [ 3 Necropsy data transfer f i l e 13 DISPATCH: , 0- ..* ...... Date:. .x%: R 1719%. ... PLEASE SIGN AND RETURN THIS FORM AS SOON AS POSSIBLE AFTER RECEIPT: COMPANY/SIGNATURE. &.%.A. .b....?B. Date:. ..fdd.?.?'C?/.%b**3# BACK TO MAIN _?*._& . ._ .. Lisa A. Clemen 1 A 0210619904:20 PM .1 Enviromntal Technology & Safely sowicdr ' ' 3M Building 02-3E-09 I (851) nama PO Box 3331 S t Paul, MN 551333331 Fax: (651) 778-6176 To: I cc: Subject Krls J. HansenlUS-Corpor~S~3M-Corporate Karl J. RarnbdUSCorporate/3M/US@3MCorporate Grant A PauUUSCorporate/3MNS@3M-C01porate NOTOX 242933 Sample Receipt 1 On 02/03/99 we received 168 rat livers from NOTOX 242933 (TOX120,W1220). These s_a-mples were receivedfrozen on dry ice and have been stored in Freezer 9 at -20 +k1OC. Lisa SAMW3 QECXUEP 4 W I F E D al aaS;&b 1' f .-\ 1 BACK TO MAIN 1 1 3M fnvi ronrnental Technology and Safety Services attn. Kris Hansen 935 Bush Avenue 1 Building 2-3E-09 S t . Paul, MN 55133-3331 USA )))NOTOX SrfrtyL Envhnmontrl Rosearch RK hmbakcnwotping 3 P.O. Box 3476 5203 DL 's-H.rtogcnborch The Notherlands ?'el.: (073) 641 95 75 Fa:(073) 641 85 43 3. Date Ref. :: 2K9IM-JAN-99 Subject: NOTOX Test Substance No. 83484, 83494, 83502, 84456, 84141 .. *. .: . . . ...a. 1 . . j Dear Kris, - ... ] On request of Hrs. S. de Hoog herewith I send the l i v e r samples (total 168) from NOTOX project 242933. With kind regards,' 1 NOTOX B.V. 3 MariQtte de Kinkelder Test Substance Offlcer 1 I * enclosure; a.m. Liver samples Chamber of Commerce 'Mertogenbosch nr. 16055281 BACK TO MAIN NOTOXp r o l e m2933 --1 .- Feb. 4,1999 The liya samplen m h listed below did not include a collectiondate on theircontainers. After readingtheprotocol, I wrote,the collection date oneach container. See below for dam. If thesecollectiondates BTC not comct, please call me at 1-165-778-5568. Regards, Lisa A clanen Isc '. Samples 1 2 3 4 17 18 19 m 33 34 35 36 49 50 51 52 65 66 67 68 81 82 83 84 Gmup 1 1 1 1 2 2 2 2 3 3 3 3 4. .4 4 4 5 5 5 5 6 6 6 6 Collection Date Added 7 11/03/98 7 11/03/98 7 11/03/98 7 iyo3/98 7 11/03/98. 7 11/03/98 7 11103/98 7 11/03/98 7 11103/98 7 11/03/98 7 11/03/98. 7 11/03/98 7 11/03/98 7 11/03/98 7 11103198 7 11/03/98 7 iim3i98 7 11/03/98 7 1m3B8 7 11/03/98 7 11103/98 7.1rn3/98 7 1l/O3/98 7 11/03/98 Sampla 109 110 111 125 . 126 . 127' 141 142 143 157 . 158 159 173 174 175 189 190 191 Group 1 1 1 2 2 2 3 3 3. .4 4 '4 5 5 5 6 6 6 Collection Date Added ? 12/04/98 3 12/04/98 7 12104/98 ?12/04/98 * 7 12/04/98 ?12104/98 . 7 12/04/98 7 12104/98 7 12/04/98 ?12/04/98. * 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 71 W 8 .'\ .. BACK TO MAIN 1 NOTOX#242933 . TOXl20 Liver Samples ReCdved,p03/99 ' Samples Group CollectionDate Samplw Group Collection Datc Samples Group CollectionDate -1 -2 1 \3 k4 `5 1 . k6 . `7 `8 1 9 ` .10 `1 1 -43 1 `14 d5 3 -17 1 iirn3~8 -65 . 5 1 iim3~8 46 5 1 11/03/98 1 11/03198 67. 5 48 5 1 1W8 d9 5 1 11/04/98 * `70 5 1 11/04/98 \71 5 1 11/04/98 172 5 1 11/18/98 . `c73 5 1 11/18/98 1 1inma -74 5 VIS 5 1 12102198 . '77 5 1. 12102198 78 ' 5 . 1 12102198 *m 5 2 ' 11103/98 `81 6 2 11103/98 6 1i m 3 m iirn3m iimm 11/03/98 11/04/98 11M8 11/04/98 11/04/98 11118/98 11/18/98 11/18/98 12/02/98 12/02/98 12iO2J98 11103198 11103/98 ' u29 3 -130 - 3 `131 3 -1 32 3 -133 3 `134 3 ~135 3 q36 3 a37 3 Y 38 3 Y39 3 `141 3 ' `142 3 `143 3 `145 4 `146 4 iimm iirns/98 iimsm iirns/98 11/06/98 11/06/98 11106198 1ltO6t98 11/20/98 11/20/98 1ll20t98 - 12/04/98 12/04/98 12/04/98 1lK)5/98 11105198 I b.19 2 '20 2 .21 2 $22 2 lyO3/98 1W8 11104(98 11/04/98 L83 6 44 6 35 6 ~86 6 11/03/98 11103198 11/04/98 11M8 447 .44a 449 q50 4 11/05/98 4 11/05/98 4 * 11/06/98 4 11/06/98 i 43 2 b24 2 `25 2 11/04/98 11/04/98 iinma \87. 6 '88 6 '89 6 11/04/98 1i/o4198 iinwa 451 4 452 4 \153 4 11/06/98 1lID6&8 11120/98 I .26 2 47 2 39 2 30 2 11/18/98 11/18/98 12/M198 12/02/98 -90 6 '91 6 93 6 `94 6 11/18/98 11/18/98 12/02/98 12/02/98 w4 4 us5 4 Y57 4 `158 4 11t20t98 11r2W8 12/04/98 12/04/98 I `31 2 -33 . 3 -34 3 1811/03/98 11103198 '95 6 . 12102/98 97 1 iimm \98 1 11/05/98 -159 4 V61 5 `162 5 12/04/98 iirnsm 11/05/98 1 1 4 1 \ 1 1 1 i e5 3 -36 3 47 3 -38 3' . 49 3 '40 3 L41 3 k42 3 `43 3 \45 3 46 3 d7 3 49 4 <so 4 L51 , 4 92 4 e3 4 , -4 4 `55 4 ~56 4 '57 4 38 4 '59 4 61 4 42 4 ~63 4' 1lrn/98 1m3/98 11M8 11104/98 Illorup8 11/04/98 iinm8 . iinma iinma 12lW98 12nJ2/98 1un/98 11/03/98 11/03/98 mma 11103198 11/04/98 11/04/98 11/04/98 11/04/98 11/18/98 1inma 11/18/98 12102198 1812102198 9 1 ~00 1 a01 1 . `lo2 1 `103 1 UO4 1 405 1 406 1 . `107 1 \109 1 k110 1 A11 1 ~113 2 414 2 915 2 16 2 `1 17 2 x118 2 ' 419 2 c.120 2 -121 2 * '122 2 `123 2 `125 2 q26 2 \127 2 11/05/98 11/05/98 11106198 11/06/98 11/06/98 11/06/98 11m8 1l R 9 8 1l n W 8 12iW98 12/04/98 12/04/98 11/05/98 11/05/98 iirnma 11/05/98 11/06/98 11/06/98 11106198 11/06/98 11/20/98 11/20/98 11m8 12/04/98 12/'r)4/98 12/04/98 `163 5 11/05/98 -1 64 5 1ur)5/98 465 5 11mm8 `166 5 iI@6/98 U67 5 11/06/98 `168 5 - 11/06/98 -169 5 1U 2 9 8 '170 5 1lJ298 V71 5 11/298 *"\173 5 12/04/98 Y74 475 - 5 12104198 5 12/04/98 ~177 6 11/05/98 -178 6 11/05/98 -179 6 1U05/98 -180 6 11/05/98 r181 6 11/06/98 `1 82 6 11/06/98 `183 6 11/06/98 ,184 6 485 6 11/06/98 11f298 \186 6 1l r 2 9 8 d87 6 11/20/98 389 6 12/04/98 `1 90 6 12/04/98 -191 6 12J04/98 I I I . .- ,.-.. . :.,., . .. 2:. ,. ... :- 1 * . .?ti. , I. .. ! y. 2. RESEARCH RV . HAMBAKENWETERING 3 Conact Nuns: .I BACK TO MAIN \. .. .. * *r : . ... . . .: ' :**: ' E!(+ . ' .c. . _.$. . . .. . .. .. . .. .. '. , .. .. . .. 004350004 961 0041500011 961 .. .. 3 : SA -- DILUTIONS SUM dd dst CT BACK TO MAIN tm SVtudye:7h5 4camt4 fo orp com SlsHWEhETem2b5r 2257 I (i. X TTT acer "| et eeBe 17 I ol HT Hr 7 11 rT wa 52 oe Lr r rr ------ EN A AC Fy } ir er| A 1 | zr | rar). =] HTT 1 ar aa IN HTT 2 A 187. 7 Te a ae [a -- rrr 11 --L AN ESe ECI Fy Ns + tntmrt + rns ST ld picts - . SR SalSapnh vot . pede TT [or -- --DILUTIONSSUMMARY BACK SHEET TO MAIN Dehn 2 gtd troRn E SoiveglTNNumber: 72467 325 Matrix:tCoer mr or mse 03 |_| a E TT s T B-- t------ wr| HT rT jms (a7 1] TTT171 ee. I tr er -- VE Tr1 t ; -- en -- T TT r T--T--1 { 1 -- _ Tr 1 ----| 1 L te tt in 1 ] BACK TO MAIN DILUTIONS SUMMARY SHEET ...-1 sample number . or dcscrpilion -'-U---A Gs LLM I BACK TO MAIN _._-. . DILUTIONS SUMMARY SHEET 714 I 724 I %-I- 73fl I [ 77.A I , 7w ' f9A J i7K G3 rm I aifl I Nolts: BACK TO MAIN sample number x I' I I I NLUTIONS . BACK TO MAIN C. DILUTIONS SUMMARY SHEET I Stpdy:TOx- 13 0 BACK TO MAIN DILUTIONS SUMMARY SmET Nota. BACK TO MAIN DILUTIONS SUMMARYSHEET DSiilnudtyioTnODaYt-e/1An2a0lyst: S/257/97-SAH ESxteravcteiornDNaateb/eAmrai:yMste:4O19|/T9 -SHAA|HPNSRP[ICP rks Ey Hn [re fe wee pee] a peo MTV low, Te ou, VT fee, | TT 797 I FS A IS I A NN I legs, | TT1] Ber | TT 1111] Jeam, TT TT Poa, | VT ar, TTT) Ped 1 ys,| | UT sm,LTV 71 1 TT TT tt 1 TT sl 7 I 1 TAPE = rr TT 1 rr ---- TTFT111 0 = 1 D1000= [ul sample + 1000ul wiethano] - - .... .*-. . . .-- ...- -.- --.- L.".-.... - BACK TO MAIN I..------ DILUTIONS SUMMARY SHEET Notcs. BACK TO MAIN I I 7br Study Number: 1 Equipment Number: Notebook# and Page: i Final SolventfIN Number: I 1 3 "3 1 I Extraction-1 Template Review Date Form Approved byDate: -\ Weight Volume Sheetl2O.xls Page 1 of I i 1 I 7 '1 1' I i 1 1 .I 1 I J J f 1 Extraction-I Template. Review Date i Sample weightsNolumes --c- BACK TO MAIN Form Approved bymate: Weight Volume Sh~etl2O.xls Page 1 of BACK TO MAIN , . Sample wei- gh- t_ sN4 olumes Study Number: 7 0 X / 2 0 1 ! Equipment Number: Notebook# and Page: ' 1 Final SolvenVfN Number: . 1 f 3 -1 1 .Notes: - Extraction-1Template Review Date Form Approved bymate: Weight Volume SheetlZOxls Page 1 of ' - Sample weig-h-t-sNolumes Study Number: 7 - b ) c f A D Equipment Number: Notebook# and Page: , Final S o l v e s m Number: BACK TO MAIN 1 I I I 1 Summary of method: 1 .J * Notes: Extraction-I Template ' Review Date b Form Approved bymate: Weight Volume Sheetl20.xls Page 1of Extraction W-o-r._ks-heet ETS-8-4.0 BACK TO MAIN Attachment'A ' ETS-84.0 Extraction of PFOS from Serum Page 0 of 3 .. Extraction Worksheet ETS-8-4.0 --t.- BACK TO MAIN I I -1 7 Attachment A ETS-8-4.0 Extraction of PFOS from Serum Page IO of 13 Extraction Worksheet ETS-8-4.0 --t- BACK TO MAIN Filter using a 3cc 8-Dsyringewith a O.2um filter into a 1.5 ml autosamolevial for a final concentration of Attachment A Em-8-4.0 Extraction of PFOS from Serum Page 10 of 13 BACK TO MAIN 1 Extraction Worksheet ETS-8-4.0 't I 7 1 1 3 1 I 1 1 I t I 1 .I I I .I I I 0 Add methanol Volume f . 0 ml - TN-A- dY&Y I I _. 1 Vortex 3 0 sec. I I 0 GIIo% ?/-Y ' s o .J - s ~ ' ~ ~ W h . ~-293ik JM 6/I &I, Filter using a 3cc B-Dsyringewith a0.2umfilter into a 1.5 ml autosample vial MSIMSD/& Cont. Checks: Spiked ULof a C ppm std 99&/-1 -/ ) for a fmal concentration of 6 aq) ppm. MSMSD used sampl Cont. Checks used same matrix as for std curve. Surrogate Standard: Spiked 10 ULof a 996'//-/6-4to all samples, standards,and blanks MSI/MID/-S~IL.W J MM M Attachment A -,ETS-8-4.0 Extraction of PFOS from Serum Page 10 of 13 BACK TO MAIN Prep Date(s): AIldySt(s) Sample Matrix MdhodlRcvbiOn: T8qcthdyte(S): 4/19/99 SAH/RWW/JCP/SRP Rat Liver FACT-M-1.0 Pow PFOS. ,PFHS.M513 and M613 FC Mix Std Approx. 0.500 ppm: FC Mix Std Approx. 5.00 ppm: FCMI.Std Approx. 50.0 m: SurrogateStd Approx. 100ppm: 99011-162 99011-16-1 99011-124 99012-3-24 Study N I I ~ C C"OX-120 Equipment Nomber: ~ lsd olvent & RJ Number: McOHI TN-~-2438 B b k TlssuefIdcntWu: RabbitLiverI99012-5-20 M Am? spiked mt 0.002 0.004 0.010 0.020 ' 0.040 0.010 0.020 0.030 0.004 . Au Density 0 0.180 0.180 0.180 0.180 0.180 0.180 0.1 ao 0.180 0.iao Calculated Concentr II P O M Finalconc. ' I119 of standards im PFOS F icork It Sample Ma& Final Conc 5.56 11.1 27.8 ' 55.6 111 278 556 a33 1111 5.56 11.1 27.8 55.6 111 I 278 556 . 833 . 1111 PPBS Final Conc. Ms13 F icorn M6U F i i cow Surrogate std conc. Au Amy Spiked 27.8 I 27.8 I 27.8 I Finalcone. I Finalconc. I 55.6 55.6 55.6 278 556 . 556 a33 833 556 ' 833 1111 1111 1111 II Ion Pair Extraction Worksheet BACK TO MAIN * - .MSMSD used s Additional Comments: PrepDate(s): Analyst@): Sample Ma* Mtthodmwirion: Target Analyte(s): 2/22/99 RWWBAH h t Liver FACT-M-1 .O WAA. PFOS,' FC Mix SM Approx. 0.500 ppm: FC Mix Std Appro%5.00 ppm: FC Mix StdAppro%50.0 ppm: Surrogate Std Approx. 16.5 ppm: PFHS, ~4513& M613 99011-16-2 . 99011-16-1 99011-124 98094-12-24 Actual Conccntmtionsof Standards in the FC Mix POAA PFOS Std Conc. std conc. Std Conc. 0.500 0.500 . 0.500 0.500 5.00 5.00 5.00 5.00 5.00 50.0 50.0 50.0 BACK TO MAIN Study Number: TOX-120 Equipment Nnmber: Final Solvent& TN Number: MeOHI TN-A-2325 Blaak~udIdentifler:Rabbit Lival98099-15-3 All Amk spiked mL 0.002 ' 0.004 0.010 0.020 0.040 0.010 0.020 0.030 0.004 M Density g 0.167 . 0.167 0.167 0.167 0.167 0.167 0.167 ' 0.167 , 0.167 MSlMSD 106F-Gl 1 IOF-GI 0.9240 0.9242 -' . 4.6 0.010 4.6 0.010 -7_- POAAQFOS, ,PFHS.MS 13,M6I3 BACK TO MAIN ._ 3 Extraction-1 Template Review Date -.=_- Form Approved bymate: Weight Volume Shcet.xls Page 1of - Sample Weights/ Volumes BACK TO MAIN .. I Notes: Extraction-1 Template ' Review Date I I -_-- FormApprovedby.- Weight Volume Shedxls . Page 1 of - Sample Weights/ Volumes BACK TO MAIN . -- Extraction-1Template Review Date i b i b .~ ~~ Fonn Approved by/Datc: Weight Volume Shectxls Page 1of- . Sample Weights/ VoIumes BACK TO MAIN 1 I 1 1 3 I SummarykMethod: f 1 II- .. .I Extraction-1TempIate Review Date i ! --_- FormApprovedbylDate: Weight Volume Shcetxls Page 1of - BACK TO MAIN 1 I SOUP 020199: FACT Equipment Log 99014 Quattro II, #6414E PAGE 1 DATE: 2/23/99 1 . ANALYST drb STUDY "MBEJa:Tox-120 MA- p a t Liver DESCRIPTION Liver Samdes 1 FACTMethod FACT-M-4.0. Injection volume: 10 uL Cycle time: 13.5 min. Electrode: Z-Sprav 1 Mobile Phase: (A)2.0 mMN H4CH3CO2 ( B ) L '1 TN-A- or stndrd number (A) 98012-1-4 Prepared (datelby): (A) 2/11/99 hoi ' (B) "N-A-2260 (B) 2/99. hoi T Expires: Sto~eatrmmtemp? (A) 8/99 or N Other: ( B ) L 1 Guard column: None i Part number: na I Lot number: na 1 Analyticalcolumn: KevstoneBetasil Size: 2 x 100mm ' Type: C18 Sphere size:_$ um 1 Part number: 105-701-2 Lot number: P8C09 Serial numk10881106D z._I. . 3 A d y t i d parameters: ESI nebulizinggas (L/hr) 15 Sheath gas (L/hr) 0 .-\ 3 Back pressure: 120bar I HPLC solvent gradient: Long 2 Time (min.1 %B 1) 0.00 40.0 1 ' '2) 8.50 90.0 3) 11.0 90.0 i 4) 12.0 5) '13.5 40.0 StOD 1 j 1 i i lvning Method Report Method: C:\MASSLYNX\FACT-l.PI?O\ACQUDB\PFOS 1 I 1 Printed : Tue Fob 23 15:59:26 1999S b k o~aom I I 1 I 1 i I i MS I I SOURCE ( ESP- ) Capi1lary Cone 1 Extractor RF Lens Source Block Temp. Desolvation Temp. 1 3 Pressures I Analyser Vacuum Gas Cell f i .I I 1 Set 3.00 60 5 0.2 150 250 Rabk 2.99 -60 -6 142 250 Rdbk 7.8e-6 . FAIL MS1 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier :\ LM Resolution HM Resolution Collision Ion Energy. Ion Energy Ramp Lens 8 Lens 9 Multiplier BACK TO MAIN Page 1 Set Rdbk 14.0 : 1 4 . 0 .,. Y 2.0 . 0.0 5 5 650 Set 15.0 15.0 45 2.. 0 0.0 40. 0 650 Rdbk 42 39 44 1 1 1 1 1 399.00 80.00 0.25 4s 60 7 2 399.00 99.00 0.25 4s 3 399.00 130.00 0.25 45 60 60 3 . I n t e r aunM1 Dolay ( See. ) 1 8p.a ( DakOM 1 S t a r t T h ( Xiru 1 LndTb(Minm): J aunM1 P u e n t D8uuht.r 0.03 0.00 0.00 13.50 1 -11 (sac.) * C o l l m u w (bV) COM ( V 1 '1 1 499.00 80.00 0.15 45 2 499.00 99-00 0.25 45 3 499.00 130.00 0.25 45 60 60 60 1 0.00 0.00 13.50 1 1 Doughtmr -11 (sen) C o l l m e w (eV) COM ( v ) i 60.00 ' 0.25 45 35 99.00 0.25 45 35 I I 1 . 1 413.00 119.00 0.15 30 15 .f 413.00 169.00 0.25 20 15 3 413.00 219{00 0.25 30 15 _1 Punation : 5 )AII O f 3 Wu. ?air. ( u.-) :'j 1 469.00 169.00 0.25 a 469.00 219.00 0.25 .' 3 469.00 119.00 0.25 ' 45 30 45 30 IS 30 3 1 I . 569.00 169.00 0.15 45 35 2 569.00 219.00 0.25 45 35 1 . 3 569.00 119.00 0.25 45 35 - I I i I 1 . .. .. - BACK TO MAIN . ?we 1 i - Masslynx Sample Llst Sample Ust: C:\MASSLYNWACT-l .PRO\SampleDB\O22399cSPL Printed: Tue Feb 23 16:25:20 1999 L o 0201%4 o&g' I BACK TO MAIN Page 1 02,/23/? ' , File Name Flle Text I MS File Inlet I Bottlc olume SampleT] ConcA ConcB ConcC C I 1 022399020 MeOH Blank TOXlPC long- 91 10 Andyte 2 022399021 MeOH Blank TOX12C long2 91 10 Analyte 3 022399022 Tox 120, RBL02229-H20Blk-1,2/22 TOX12C long2 1 10 Analyte 4 022399023 Tox 120, RBL02229-RBLiverBlk-1,2 TOXl2C long2 2 10 Analyte 5 022399024 TOX120, RBLO2229-5ppbls2/22/99 TOX12C long2 3 10 Standard I 6 022399025 Tox 120, RBL02229-1Oppb-1,2/22/9 TOXl2C long2 4 10 Standard 7 022399026 Tox 120, RBL02229-25ppb-l12/22/9 TOXl2C long2 5 10 standard 8 022399027 Tox 120, RBL02229-50ppb-l,2/22/9 TOXl2C long2 6 10 Standard 9 022399028 Tox 120, RBLO2229-1OOppb-1,2/22/ TOX12C long2 7 10 Standard 1 10 022399029 Tox 120, RBL02229-250ppb-1,2/22/ TOX12C long2 8 ' 10 Standard 11 022399030 Tox 120, RBLO2229-500ppbl,2/22/ TOXl2C long2 9 10 Standard 12 022399031 Tox 120, RBL02229-750ppbll2/22/ TOX12C long2 10 10 Standard 13 022399032 Tox 120, RBL02229-1000ppb-1,2/22 TOXl2C long2 11 10 Standard 1 14 022399033 MeOH Blank TOX12C long2 92 10 Analyte 15 022399034 TOX120DNO. 97F-G1,2/22/99 TOX12C long2 12 10 Analyte. . . 16 022399035 TOX120DNO. 98F-G1,2/22/99 TOXl2C long2 13 10 Analyte 17 022399036 TOX120DNO. 99F-Gl,2/22/99 TOXl2C long2 14 10 Analyte i a 022399037 Tox 120DNO. 1OOF-Gl,2/22/99 TOXl2C long2 15 10 Analyte 1 19 022399938 Tox 120DNO. 101F-G1,2/22/99 TOXl2C long2 16 10 Analyte 20 022399039 Tox 120, RBL02229250ppbCCl , 2/ TOX12C long2 17 10 QC 21 022399040 MeOH Blank TOXl2C long2 92 10 Analyte . 1 1 22 022399041 Tox 120DNO. 102F-01,2/22/99 TOXl2C long2 18 10 Analyte 23 022399042 Tox 120 DNO. 103F-G1,2/22/99 TOX12C long2 19 10 Analyte 24 022399043 Tox 120DNO. 104F-01,2/22/99 TOXl2C long2 20 10 Analyte 25 022399044 Tox 120 DNO. 105F-G1,2/22/99 TOXlPC long2 21 10 Analyte 26 022399045 Tox 120DNO. 106F-G1,2/22/99 TOXl2C long2 22 10 Analyte I 27 022399046 Tox 120, RBL02229-250ppb-CC2,2/ TOX12C long2 23 10 Qc 28 022399047 MeOH Blank TOXl2C long2 92 10 Analyte 29 022399048 Tox 120DNO. 107F-G1,2/22/99 TOXl2C long2 24 10 Analyte 30 022399049 Tox 120 DNO. 109F-G1,2/22/99 TOX12C long2 25 10 Analyte 31 022399050 Tox 120DNO. 11OF-G1,2/22/99 TOXl2C long2 27 10 Analyte 1 32 022399051 Tox 120 DNO. 111F-G1,2/22/99 TOXl2C long2 28 10 Analyte -2 33 022399052 Tox 120, RBL02229-250ppb-CC3,2/ TOXl2C long2 29 10 QC 34 022399053 MeOH Blank TOX12C long2 93 10 Analyte 35 022399054 Tox 120 DNO. 113F-G2,2/22/99 TOXl2C long2 30 10 Analyte 1 30 022399055 Tox 120 DNO. 114F-G2,2/22/99 TOXlPC long2 31 10 Analyte 37 022399056 Tox 120 DNO. 115F-G2,2/22/99 TOXl2C long2 32 . 10 Analyte 38 022399057 Tox 120 DNO. 116F-G2,2/22/99 TOX12C long2 33 10 Analyte :I 39 022399058 Tox 120DNO. 117F-G2, W 9 9 TOXl2C long2 34 10 Analyte 40 022399059 Tox 120, RBL02229-250ppb-CC4,2/ TOXlPC long2 35 10 QC 41 022399060 MeOH Blank TOXl2C long2 93 10' Analyte 42 022399061 Tox 120 DNO. 118F-G2,2/22/99 TOX12C long2 36 10 Analyte 43 022399062 Tox 120DNO. 119F-G2,2/22/99 TOXl2C l o n g 37 10 Analyte 44. 022399063 Tox 120 DNO. 120F-G2,2/22/99 TOX12C long2 38 1 45 022399064 .Tax 120 DNO. 121F-G2,2/22/99 TOXl2C long2 39 46 022399065 Tox 120 DNO. 122F-G2,2/22/99 TOXl2C long2 40 47 022399066 Tox 120, RBL02229-250ppb-CC5,2/ TOXl2C long2 41 -48 022399067 MeOH Blank 1 49 022399068 Tox 120 DNO. 123F-G2, 2/22/99 50 022399069 Tox 120 DNO. 125F-G2,2/22/99 TOXl2C long2 93 TOXl2C long2 42 . TOXl2C long2 43 51 022399070 Tox 120 DNO. 126F-G2,2/22/99 TOXl2C long2 44 52 022399071 Tox 120 DNO. 127F-G2,2/22/99 TOX12C long2 45 10 Analyte 10 Andyte I O Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 1 53 022399072 Tox 120 DNO. 129F-G3,2/22/99 TOXl2C long2 46 54 022399073 Tox 120, RBL02229-250ppb-CC6,2/ TOXl2C long2 47 55 022399074 MeOH Blank 56 022399075 Tox 120 DNO. 130F-G3,2/22/99 TOXl2C long2 94 TOXl2C long2 48 57 022399076 Tox 120 DNO. 131F-G3,2/22/99 TOXI2C long2 49 10 Analyte * 10 QC 10 Andyte 10 Analyte 10 Analyte J 58 022399077 Tox 120 bNO. 132F-G3,2/22/99 I 59 022399078 Tox 120 DNO. 133F-G3,2/22/99 TOXl2C long2 50 TOXl2C long2 51 10 Analyte 10 Analyte 1 i 7 1 1 1 ] 1 1 I I 1 I 'I .I I I ..I 'I 1 Masslvnx - SamDle LLt Sample Ust: C:\MASSLYNXFACT-l .PRO\SampleDB\O22399cSPL Printed: Tue Feb 23 1625:20 1999 S O l l b 020144 BACK TO MAIN A/A hlm 0 2 8 fI 4 7I 9 Page 2 File Name File Text MS File inlet I Bottlc olume SampleT] ConcA Conc B Conc C ( 60 022399079 TOX120DNO. 134F-G3,2/22/99 TOXl2C long2 52 61 022399080 Tox 120, RBL02229-250ppb-CC7,2/ TOXl2C long2 53 62 022399081 MeOH Blank TOXl2C long2 94 63 022399082 Tox 120 DNO. 135F-G3,2/22/99 TOXl2C long2 54 64 . 022399083 Tox 120 DNO. 136F-G3,2/22/99 TOXl2C long2 55 65 022399084 .Tox 120 DNO. 137F-G3,2/22/99 TOW 2C long2 56 66 022399085 Tox 120 DNO. 138F-G3; 2/22/99 TOX12C long2 57 67 022399086 TOX120DNO. 139F-G3,2/22/99 TOXl2C long2 58 68 022399087 Tox 120, RBL02229-250ppb-CC8,2/ TOX12C long2 59 69 022399088 MeOHBlank - . TOXl2C long2 95 70 022399089 TOX120DNO. 141F-G3,2/22/99 . TOXl2C Ion02 60 71 022399090 Tox 120 DNO. 142F-G3,2/22/99 TOXl2C long2 61 72 022399091 TOX120 DNO. 143F-G3,2/22/99 TOXl2C long2 62 73 022399092 Tox 120, RBL02229-250ppb-CC9,2/ TOXl2C long2 63 74 022399093 MeOH Blank TOXl2C long2 95 75 022399094 Tox 120, 106F-G1-250ppb-MSD2 E 5 TOXl2C long2 64 76 77 022399095 022399096 TOX120,106F-G1-250ppb-MSD, 2/2 TOX120, RBL02229-250ppb-CCl0, < TOX12C TOX12C long2 long2 65 66 78 022399097 MeOH Blank TOXl2C long2 95 79 022399098 Tox 120, RBL02229-H20 Blk-2,2/22 TOX126 long2 67 80 022399099 Tox 120, RBL02229-RBLiverBlk-lD2r TOXl2C long2 68 81 022399100 Tox 120, RBL02229-5ppb-2,2/22/99 TOXl2C long2 69 82 022399101 Tox 120, RBLO2229-1Oppb-2,2/22/!3 TOXl2C long;! 70 83 022399102 TOX120, RBL02229-25ppb-2,2/22/9 TOXl2C long2 71 84 022399103 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72 85 022399104 TOX120, RBL02229-1OOppb-2, 2/22/ TOXl2C long2 73 86 022399105 Tox 120, RBL02229-250ppb-2,2/22/ TOX12C long2 74 87 022399106 Tox 120, RBL02229-500ppb-2,2/22/ TOXl2C long2 75 88 022399107 TOX120, RBL02229-750ppb-2,2/22/ TOXl2C long2 76 89 022399108 Tox 120, RBL02229-1OOOppb-2,2LZ TOXl2C long2 77 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Qc 10 Analyte. 10 Analyte 10 Analyte ' 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 standard 10 Standard 10 Standard 10 Standard .. 1 I 1 I 1 3 1 7 I I nalativa h . i * t I ' Abaoluta hmimhts 1 nalativa U U I AhaOlIlC. UYi I 3 1 1 1 1 1 i 1 1.000000. l.OOOO00 1.000 Dimrbld 6.563. 0.000. Prvnbdldd 1.111 Yurre 10.00 Dinbld 10.00 50.00. Pvbld 0.00, M..bld 3.00. -d 0.00, DI..blad' BACK TO MAIN ._. .?i . 1 i 1 i 1 1 3 ' 3 Wt.. l* 1 &&la 1 a i 30.00 70.00. 4-00 !I 10.00 I DIUbld 10.00 ~ . O O . Rublad 0.00. D i u h l d 1 J.00, pyblod 0.00. M m b l d 1 -.I I ... I I i 1 1 I . BACK TO MAIN ..i *; unr U V ~ W I Usor h.lr hctor: mtoqrmco w i d o w TaocorI 1.000000, Dl..bld 1.000000 z.000 I 189 Dlnbld 1 a 30.00 70.00' 4.00 10.00 Mubld 10.00 50.00. publd 0.00. Dlubld J.00. Eubld 0.00, Db-bld ' BACK TO MAIN .2. 1 ! 1 v.u u Valuml US= w rutor: 1 mnC.grat. W i d w Taotor: 3 J 1 1 I J o b v d l y Ck..bold: R8.AIN p n k c.ilimJ* W.. budh wctrnl D r u wrricrl thnmholdi ! ! Rt-t .houl&ni .bruldu throwhold: i Il.1rti.r bight1 AbOlUt. bight. 1 .IU1rtiw holut. U -I Y I 1 1 I.OOOOOO. 0i.rbi.d 1.000000 1.000 30.00 10.00. 4.00 10.00 Di.rb1.a 10.00 so.00. Pvbld 0.00, Di..bld a 0 0 -.a 0:oo: DiS.bld' 1 I* 1 1 't . ..... . . BACK TO MAIN 1.000000. niubld 1.000000 a.ooo ?.410. -1d 0.000, Diubld 0.Ym -mt IIS mnbld a1 . 30.00 70.00. 4.00 10.00 m4.a 10.00 s0:oo. 8.bl.d 0.00. M..bld 3.00, 0.00. m-1m4 a d BACK TO MAIN .:$ . FACT: DATA REVIEW SY- FORM .. BACK TO MAIN Analytc 2: /JFdS BACK TO MAIN i i 1 ! 1 1 1 i I I ! i i I 1 p 3 I 1 1 1 em- 1022399020 2 022399021 3 022399012 4 022399023 5 022399024 6 022399025 7 022399026 8 022399027 9 022399028 10 022399029 11 022399030 , 12 022399031 1 3 022399032 14 022399033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399038 20 022399039 21 022399040 22 022399041 23 022399042 24 022399043 25 022399044 26 022399045 29 022399048 30 022399049 31 022399050 32 022399051 33 022399052 34 022399053 35 oiJ~9gosc 36 022399055 37 022399056 30 oiiisoos7 39 022399058 40 022399059 41 022399060 42 022399061 43 022399062 44 022399063 45 022399064 46 022399065 47 022399066 48 022399067 49 022399068 50 022399069 51 022399070 52 022399071 I 3 022399072 S4 012399073 55 022399074 56 022399075 57 022399076 58 022399077 59 021399078 60 022399079 61 022399080 62 022399081 63 022399012 64 022399083 65 022399064 66 022399085 67 02239086 68 022399017 69 022399088 70 022399089 .71 022399090 72 022399091 73 022399092 7 4 02239Y093 75 022399094 76 022399095 77 022399096 78 022399097 79 022399098 80 022399099 81 022399100 82 022399101 83 022399102 84 022399103 85 022399104 86 022399105 87 022399106 86 022399107 89 022399108 8m.li8t T U e Meoli Il* W E Blank Tox 120. RBLO2229-810 Ilk-1, 2122199 Tox 120, nrW2229-ULivmrBlL-1, 2122199 Tox 120. RBLO2229-5-1. 2/22199 Tox 120. RBLO2229-10-1, 2122199 Tox 120, UBLO2229-25-1, 2122199 Tox 120. RBLO2229-5-1, 2122199 Tox 120. RBLO2229-lOOppb-1. 2122199 TOX 120, RBLO2229-25Oppb-1, 2122199 Tox 12'0. larn2229-5OOppb-1, 2l22L99 Tox l20, lllLo2229-75Oppb-1, 2122/99 Tox 3.20. lllLo2229-1OOOppb-1, 2122199 w 1lMk Tox 120 PR. 7?-Gl, 2122199 TOX I 2 0 ma. Y8141, 2122199 _ _ 'pax U O - P I O . 99T41. 2122199 ox Go DID. ioor-ci.-ziizigg Tox l20 DID. 1olT-01. 2/22/99 TUX 120. R B L 0 2 2 2 9 - 2 5 0 ~ C-c211.22199 mOl-ii1;nlr ~ TOX U O PR. 102141. 2122199 Tox 110 OUD. 1031-W. 2122199 TOX i z o LWO. iotr-or; 2122199 Tox X20 DID. 1051-01. 2122199 'pax 120 ma. 106r41. 2121199 TOX iio, 1~)~02229-250pPb-cQ21,22199 neon BlU* Tox a20 W. 107T41. 2122199 'Tax 120 OUO. 109?41, 2122199 To% 120 m. 110.41. 2122199 Tox 120 OUD. llY41, 2122199 Tar 1 2 0 , MtO2229-25Ogpb-CC1, 2122199 X.QII Blank ~ a 1r50 PR. ii3~-4, 2122199 Tox 120 Dlso. 1141-4, 2122199 Tax 120 Duo. 115?-01, 2122199 Tux 120 Duo. 1161U. 2122199 Tox 120 W . 1171-61, 2122199 m x 120. uILO2229-250ppb-CfXl 2122199 xaou 1t* ' Tox 120 DR). 1 1 8 1 U , 2122199 Tox 120 Mo. 1191-6. 2122199 Tox 120 DM). l2OT-U. 2122199 TOX 120 Duo. l211-01. 2122199 TOX 120 OUD. 1 2 3 1 4 , 2122199 Tox 120 Duo. l251-Q. 2122199 Tox 120 PIO. 1161-02. 2122199 Tox 120 PI). 13014. 2112199 Tox l20 DR). 1 3 Y 4 3 , 2122199 Tm 120 m. 1 3 2 1 4 , 2122199 %O' X 120 DYD. U 3 1 4 . 2122199 . TOX l20 DIO. 134143. 2122199 TOX 120, ~m.oz229-2soppb-ccr.a m i 9 9 x.08 1lurL Tox 1 2 0 EK). 1351-03. 212tE99 'pax U O PIO. 1361-03. 2l7.2199 30 EK). u71-03; 2122199 3a1 WO. 138?-W, 2122199 30 PI). u91-03, 2122199 ;a .2I01 ~L02229-250ppb-cC8.2122199 I .20 WO. 141?43, 1122199 30 W . 1 4 2 1 4 3 . 2122199 .2 0 Mo. 143193. 2122199 ;a .2110 RBL02229-250~CC9,2122199 8 .20 106?-01-25Oppb-US, 2/22/99 .20 , 106191-250ppb-NSD, 2122199 .2I10, uILO2229-2SO~b-CCl0,2122199 I urL , 30 m ~ 0 2 m - r n oaa-2, ziazi99 -20 , RBLO2129-MLiw1lk-1. 2122199 .20 llilLO2229-5e2, 2122199 .a0 , RBLO2229-10ppb-2, 2122199 20 a RSL.01229-25&-2. 2122149 120. 120, 120. 120. 120, rOu0 020199:: wuhtitatd d grintad by m m 2I25I99 Qc AMlYt. --.i- 8td DM n3 8.041 5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 250.00 250. OF 250.00 250.00 250.00 250 .OO 250.00 250.00 250.00 250.00 8 .206 8.206 8.100 8.208 8;205 6.209 8.210 8.209 8.109 8.205 8.294 8.208 8.U3 8.U4 1.033 8.P39 6.221 8.114 8.038 8.038 8.039 8.039 7.953 7.781 7.770 7.776 8.040 8.l24 8.037 8.041 8.038 8.036 7.955 8.124 1.041 8.037 8.041 8.039 8.033 8.U2 8.035 8.032 8.037 7.952 8.040 8.11s 7.951 8.03s 8.038 8.040 8435 8.039 7.949 8.036 8.036 6.036 7.950 8.041 7.948 7.9% 7.953 8.039 7.929 7.929 5.00 10.00 25.00 50.00 100.00 050.00 500.00 750.00 1000.00 8.080 8.028 1.954 6.037 7.953 8.031 7.952 7.951 8.031 7.998 Arma 3 65 373 835 554 987 1861 3063 7562 14718 21303 26747 568a 2-3 2569 2110 255046 5885 2401' 2l26 2013 3628 3302 S644 2541 10014 11101 7766 6261 1410 1212 1318 1315 2567 6615 1874 u47 ll28 1278 1191 6476 1630 1567 1936 1768 1256 6418 1366 1719 1449 1682 1540 5889 la79 159) 785 625 1536 6171 1492 1435 1409 5349 8399 9914 6209 3.62 19.89 10.00 25.23 55.98 98.29 256.57 508.35 740.04 931.56 190.63 - W 298 m u 0 LOI 1W . 11 W -2 m 3 2W ' -1 Irm -7 #P 64.75 197.57 -21 lg 74.98 65.30 61.32 iii.i6 106.67 169.07 yI( lg lg lg lg -24 W 79.89 342.84 381.10 263.76 210.80 bb W W W -16 W 40.12 34.21 36.89 36.76 80.81 223.26 bb )01 loa IB( W -11 BM 56.43 37.91 30.18 35.47 35.92 218.37 lUS BQ4 W )oI lUS -13 lUS 47.85 mI 45.63 f bb 58.62'1 * W 52.70 a 34.68 lR4 216.31 -13 BM 36.58 50.97 4a.47 49.70 44.69 197.71 a PI 101 1oL bb -21 PI 35.51 46.75 18.11 19.51 44.56 207.62 43.00 41.01 40.08 178.70 IW W n bb 10( -17 . 101 m yI( -29 W 286.03 339.32 208.97 LOI u4 -16 IOI 161 396 496 924 1576 3151 7344 14166 21260 31230 0.00 4.44 7.96 23.03 45.96 101.37 248.90 488.92 739'.22 1089.31 bb -11 u4 -20 PI -8 -8 101 1 0 W -2 101 -1 bbX 9 WWX BACK TO MAIN ! i I 1 1 1' a ! I I 1 I 1 1 1 1 f 1 1 (I- 1022399020 2 022399021 3 022399022 i OZzj99023 5 022399024 6 022399025 7 022399026 I022399027 9 022399028 10 022399029 11 022399030 ii 022399032 13 022399030 14 022399033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399038 20 021399039 21 022399040 22 022399041 23 022399042 a i o22399otJ 25 022399044 26 022399045 27 022399046 28 022399047 29 022399048 30 022399049 31 022399050 J i 022399051 33 022399052 34 022399053 35 022399054 36 022399055 17 022399056 38 022399057 39 022399058 40 022399059 41 022399060 42 022399061 43 022399061 44 022399063 45 022399064 46 022399065 47 022399066 48 022399067 49 022399068 50 022399069 51 022399070 52 022399071 53 02a399072 54 oaa399073 I S 022399074 56 022399075 57 022399076 58 022399077 59 022399078 60 022399079 61 012399080 62 022399081 63 022399082 64 022399083 65 022399084 66 022399085 67 022399086 68 022399087 69 022399088 70 022399089 971 022399090 72 022399091 73 022399092 74 022399093 75 022399094 76 022399095 77 022369096 78 022399097 79 022399098 80 022399099 81 022399100 82 022399101 83 022399102 84 022399103 85 022399104 86 022399105 87 022399106 88 022399107 89 021399108 8.91mllmt hxc Mea Blulk Mmoa Blank Tox 110. RIIL02229-MZO Blk-1. 2122199 Tox l20; RBLO2229-U)LLvmrBlk-l, 2122199 Tox 120. RBLO2229-5ppb-1, 2/22/99 Tox 110. ML02229-10-1, 2122199 ox 120; RBLoZZ~S-P~-+~;2122199 Tox 120. lULO2229-5Oppb-1. 2122199 TOX 120. RBL02229-100ppb-1. 2122199 To* 120. RBLO222Y-250-1, 2122199 T o X 120. RI)Lo2229-~ooppb-1,2/22(99 1Fox l20, RBL02229-750-1, 2122199 Tox 1 1 0 , NW2229-1OOOppb-1. 2122199 lwul Blank TOX-l26 DND. 97141, 2l22199 Tox 120 DM). 98141, 2122199 Tox 110 LOR. 99141. 2122199 TOX Uo m.ioor-ci, 2122199 Tox 110 DWO. lOlI?-Gl, 2122199 Tox 120. RI)L02229-25-O-~~b-CCl2.122199 ~ u - i l ; n L~ ~~ Tox 110 DW. 102PG1, 2122199 TOX 120 DWO. 1031-Of, 2122199 TOX 110 DLR). 1041-Gl, 2112199 Tox 110 DL30. 105?41, 2122199 Tox 110 LOR. 106141. 2122199 Tox 1 1 0 . RBLO2229-25OPph-M. 2122199 moll Blank TUX 120 DUO. 107?-C10 2122199 'Tox 120 DWO. 1091-61,.2122199 Tox 120 Iwo. 1101-01. 2122199 .. xaag Blank Tor 120 m.113142, 2/22/99 Tox 120 DISO. 1141-4, 2122/99 Tox 120 DIX). 1151-61. 2122199 TOX 120 DUO. I~SP-GZ,ziaz199 To* 120 CNO. 117?-62, 2/22/99 TO* 120. R B L O Z ~ ~ ~ - ~ ~ O P P2~1z-aC19C9~ . w.o8 ~ 1 . n ~ . Tox 120 DNO. 1181-GZ. 2122l99 ox U o DNO. ii91-0i 2122199 Tox 1 1 0 DXU. 1201-02. 2122/99 Tox 120 Duo. l211-4. 2122199 Tox 120 Duo. lZ21-Gl; 2122199 Tax 120. RBLO2229-25Oppb-CC5. 2122199 mou Blank TOX 120 DNO. 1231-62. 2l22199 TOX 120 DNO. 125T-Q. 2122199 Tor 120 m.1261-GZ. 2122199 T o x i20 DM. u 7 r - a ; 2/22/99 T O X l20 DUO. 1191-63, 2122199 l o r 1a0. ~n~oaaa~-zsoopb-ccacm,a/9s El& Tox 120 m.130Tlw. 2/22/99 T m l2 0 Dwo. 1311-03. 2122199 T a x l.PO W. 132143. 2122199 T o x l30 DUO. 133T-G3, 2122199 T a x 330 Duo. 1341-C3, 2122199 T o x l20. MLO2229-2SOppb-ECI. 2122199 H.011 Blank T a x 12 0 Dw. 1351&, 1122199 . T o x l2 0 ry0. 136143, 2122199 T a x 3a0 DISD. l37?-03, 2n2/99 Tox 1ao m. 138.-03, z m 0 9 T o x l2 0 W O . 1391-G3, 2122199 T a x 32 0 , RBW2229-250ppb-CC8, 2122199 H.011 El& Tox I.20 m.1411-G3, 2122199 T m l.io DUO. im-03. 2112199 T a x 13 0 LOR. 143143, 2122199 T o x l2 0 . RBLO2229-250ppb-CC9, 2122199 nmou El& T a x l2 0 , 106141-25O~b-1QI,2122199 Tux I2 0 , 106?-01-250ppb-wsD~2122199 T o x lLOO, RBL02229-250opb-CC10~2122199 w.08 El- TOX l20I RBL02229-1120 Elk-2, 2122199 Tox 120. RIILO2229-RIILhmrElk-1. 2122 I99 TOX 120, RBL02229-bppb4, lllll99 Tox 120, RILO2229-10ppb-2, 2122199 Tox 120, RBLO2229-25opb-2, 2122199 TOX 120. RBLO2229-5Ov~b-2,2122199 Tox 120. RBL02229-100-b-2, 2121199 TOX 120, RBL02>29-2SOp0b-2, 2/21/99 TOX 17.0, RI)L02229-50Owb-2,z i a z m TOX 120I 1BLO2229-75Oppb-2, 2122199 Tox 120. RBL02229-1000ppb-2, 2122199 ac &&alVt. 'pc AMlYt. pc AMlYt. AnAlyt. AnAlytr stbndud standard standud standud stud Standard stuulud Standard standard 5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 .. 250.00 6.119 6.821 6.lU 6.819 6.818 C.820 6-734 6.120 6.735 6.817 6.905 7.595 6.644 6.651 ' 250.00 7.594 6.650 6.565 240 678 301 545 1014 2165 5727 10773 15322 20746 254 3.60 24.13 6.46 17.91 39.86 93 .78 260.71 497.13 710.29 964.42 4.25 #I 383 1111 -35 bb -28 1111 -20 1111 - 6 Io( 4m '-1 1111 -5 m u -4 m u .. . 60 190 0.00 34.06 4553 205.70 -18 1111 . .72 0.00 654 23.01 \ bb bb 3582 160.19 -3s IDI 7.507 250.00 ' 150.00 6.565 6.735 7.596 6.652 6.559 SO.OO 6.652 101 0.00 . bb I 3329 103 34 92 3818 1411.34 0.00 0.00 0.00 171.27 -41 bQl bb rn -31 101. k <i 'C . 4340 195.72 -22 1111 150.00 6.649 6.651 3.564 7.419 250.00 250.00 250.00 6.564 6.565 7.597 7.505 6.564 6.650 6.455 6.455 5.00 10.00 15.00 50.00 100.00 250.00 500.00 750.00 1000.00 6.639 c.se 6.563 6.564 6.561 6.565 6.562 6.560 6.614 248 lZ5 3744 3.96' l4M 0.00 m 167.80 -33 191 80 0.00 bb 100 3096 59 59 2805 3249 12067552 . 0.00 137.40 0.00 0.00 113.80 144.61 689.6707 101. -45 1111 . bb bb -50 1111 1111 -64 IOI 130 107 452 925 1701 3784 7436 10539 15696 0.00 2.05 13.54 35.68 72.05 lS9.67 340.79 486.19 727.78 -100 m -79 MHX -46 m -29 W -28 bRK -32 )(IIx -32 I(wx -35 MtU -27 l4NX 8.r~~1.Llmtx C X \ Y U ~ L I ~ O L \ ? C Z - ~ . . I O \ ~ . ~ ~ O D . \ O ~ ~ ~ ~ ~ ~ w t m f l a d r Thu ?ab 25 13x24~271999 x.thDdx CxM8L~\?CZ-l..IO\Y.t\0223990 w t rodiflad: Thu ?ab 25 13x25~141999 Job cod.: mi8bt.d: Thp ?eb 25 13843x49 1999 1 i 1 I 1' 1 I 3 1- 1 1 I 1 1 1 I cawo&3* mAA 8 S.r n1022399020 022399021 3 022399022 4 022399023 5 022399024 6 022399025 7 022399026 8 022399027 9 022399028 10 022399029 11 022399030 12 022399031 13 022199032 14 022399033 15 022399034 16 022199035 17 022399036 18 022399037 19 022199038 20 022399039 21 022399040 22 022399041 23 022399042 24 022399043 25 022399044 26 022399045 27 022399046 18 ozzJ99or7 29 022399048 30 022399049 31 022399050 32 022399051 33 022399052 34 022399053 35 022399054 36 022399055 37 022399056 38 022399057 39 b22399058 40 022399059 41 022399060 42 022399061 43 022399062 44 022399063 45 022399064 46 022399065 47 022399066 48 022399067 49 022399068 50 022399069 51 022399070 52 022399071 53 022399072 54 022399073 55 022399074, 56 02239907s 57 022399076 58 022399077 59 022399078 60 022399079 61 002399080 62 022399081 63 022399082 64 ozz399o8i 65 022399084 66 022399085 67 032399086 68 022399087 69 022399088 70 022399089 71 022399090 72 02239909i 73 022399092 74 022399093 75 0223P9094 76 022399095 77 022399096 76 022399097 79 022399098 80 022399099 81 022199100 82 '022399101 83 022399102 84 022399103 85 022399104 86 022399105 87 022399106 88 022399107 89 022399108 m.1i.t Taut w.OX Blank w.OX Blank TOX 120. RBLO2229-810 Blk-1, 2/22/99 TOX 120. RBL02229-MLiveralk-1, 2/22/99 Tox 120, RBLO2229-5ppb-1. 2/22/99 TOX 120. RBLO2229-10UUb-1, 2/22/99 . To% 120. RBL02229-25mb-1. 2/22/99 TOX 120; RBL02229-50wb-1; 2/21/99 Tox 110. RBLO2229-100ppb-1. 2/22/99 Tox 120. MLO2229-250mb-1. 2/22/99 TOX 110;~~~nzng-soo-&t+-i;zizzi99 Tax 120, RBLO2229-750-1, 2/22/99 Tox 110, nBL02229-1000ppl+-1. 2/22/99 Ne08 Bl8nk Tax 120 PR. 97141. 2/22/99 . Tax u o Duo. 98.41. 2/22/99 Tox 120 DNO. 99?-01, 2122199 Tox 110 Mo. 100141. 2/22/99 Tax u o Iem. lOl?-Gl, 2/22/99 mx UO. RBLO2229-250ppb-n. 2122199 m a ~1.n~ Ibx 120 DNO. 102?41, 2/22/99 Tox 110 Duo. 103?41. 2/22/99 TUX 120 DNO. 1041-01, 2/22/99 Tox 120 DNO. 105?-G1. 2/22/99 Tox 120 Mo. 106P41. 2/12/99 "0% 120. RBL02229-250ppb-CQ. 2/22/99 XeW B l m L Tox 120 UNO. 107?41, 2/22199 ' T a x U O DNO. 1091-Gl. 2/22/99 Tox 1 1 0 DNO. 110?41# 2122199 Tox 120 DNO. lll?-Gl* 2/22/99 To% 120. RBL02229-25oopb-CC3, 2/22/99 ma011 Blank Tox 120 Duo. 113?42. 2/22/99 Tax 1 1 0 Duo. 114?-G2: 2/22/99 Tox 120 DNO. 115?4, 2/22/99 Tox 120 DNO. 1 1 6 ? 4 , 2/32/99 _ _ To% 120 DNO. 117?-62. 2122199 Tox 120. RBLO2229-25O~b-CC4.2122199 ,meox.ni& TO% 120 DNO. 118?-GZ. 2/22/99 Tox 120 DNO. 119142. 2/22/99 Tor 110 Duo. 1 2 0 ? 4 , 2/21/99 Tox 110 m.12Ll-Q. 2/22/99 Tox 110 Dm. 1221-02. 2/22/99 Tax 110. RBLO2229-25Oppb-rrS. 2122/99 XeW Blank To% 110 Dm. U3?-01. 2/22/99 20 DNO. 125?-Q; 2/21/99 2,20 RIO. 116?-Q. 2/22/99 0 m.127?-Q. 2122/99 30 mw. 119F-03. 2/22/99 20 , ~zzz9-zsopob-ccc. a/zz/99 BIlmk 3.a 0 m.110?-03, 2122199 10 D100. 131?43, 2/22/99 a.a10 0110. l32bG3, 2/22/99 0 Dlso. 133.43. aio Mo. l34.43, 2/22/99 2/22/99 * 310. RBLO2229-250pob-CcI. 2/22/99 1 IlUIk a110 0110. l35?-&. 2/22/99 10 DK). 136?-03, 2/22/99 U10 m.137?-GJ. 2I22I99 Tax l20 0110. 138?-03: 2/22/99 Tox 120 DNO. 139?-G3, 2/22/99 Tox 120, RBLO2229-250ppb-Fc8,2122/99 , W X Blank Tox l20 0110. 141P-G3. 2/22/99 'pox 120 W . 142?-01. 2/22/99 Tox 1 2 0 DNO. 143?43, 2/22/99 Tox U O , RBLO2229-25Oopb-CC9, 2122199 ne08 Blank TOX 120, iosr-ai-zsoopb-us. zizw99 Tox 1 1 0 ; 106?41-250pph-nSDI 2/22/99 Tox 120, RBL02229-250ppb-CC10, 2/22/99 HOOX BlU* Tox 120. RBLO2229-810 Blk-2. 2/22/99 TOX 120, RBLO2229-RBLivmrBlk-1. 2/22/99 TOX 120. RBLO2229-Snb-2, 2/22/99 fox 120. RBLO222S-10ppb-2, 2/22/99 TOX 220. RBLO2229-2Sppb-2, 2/22/99 Toox 120. RBLo2229-soppb-2. 2/22/99 Tox 120, RBL022?9-100ppb-2, 2/22/99 Tox 120. MW2229-25oppb-2, 2/22/99 Tox 120, RBLO222Y-5ooppb-2, 2/22/99 Tax 120. RBL02229-750ppb-2, 2/22/99 Tox 120. RBLoz229-1oooppb-2, 2/22/99 BACK TO MAIN 8td con0 RT 7.462 5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 -7 452 7.459 7.456 7.460 7.459 7.460 7.459 7.457 &. J r u 81 amv ?1W. 14.18 101 65 120 184 396 839 1650 2354 3013 9.37 25.91 44.89 108.09 240.35 481.92 691.87 888.41 -6 IUt 4 !a -10 !a 8 la -4 !a -4 10( -8 -11 ma 250.00 7.291 732 208.43 -17 10( 250.0Q 7.290 715 203.33 -19 !a 250.00 250.00 250.00 7.291 7.461 7.374 7.290 7.374 7.288 7.206 7.373 7.377 7.373 7.378 7.291 7.284 7.459 aso.00 250.00 7.289 ?-a91 7.366 7.287 7.287 7.289 7.290 7..:*89 7.376 7.285 250.00 7.287 853 244.41 -2 II* 474 131.52 bb 425 116.90 m4 517 162.06 m 571 160.41 bb 647 183.02 . IUt 755 215.34 -14 II* 686 194.56 101 779 222.27 bb 511 142.52 101 115 24.42 bb 49 4.60 bb 710 219.81 -12 m 92 17.55 6 lOl t 260 61.49 1pI 777 221.8s -11 #I 386 105.09 m 348 93.73 , m4 586 16S.30 .bb 452 124.79 m 334 89.57 m 73 6 209.50 -16 lQ4 602 169.49 bb 285 75.09 bb ,890 255.58 2 gl 250.00 250.00 7i204 7.376 7 .181 7.180 5.00 10.00 25.00 50.00 100.00 250.00 500.00 150.00 1000.00 7.191 7.288 7.290 7.281 7.291 7.288 1.286 7.248 668 1038 674 685 189.16 299.56 190.96 194.25 -24 )01 101 101 -22 10( 67 127 174 400 854 1786 2594 3872 10.17 27.94 42.08 111.89 244.13 522.67 163.60 1144.51 2 Id 12 IQL -16 Io( 12 bb -2 !a 5 BM 2 14 LDIY BACK TO MAIN 3 022399022 4 022399023 5 022399024 6 022399025 7 022399026 8 022399027 9 022399028 10 022399029 11 022399030 12 022399031 13 022399032 14 022399033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399038 20 022399039 21 022399040 22 022399041 23 022399042 U 022399043 25 022399044 26 022399045 27 022399046 28 022399047 29 022399048 30 022399049' 31 022399050 32 022399051 33 022399052 34 022399053 35 022399054 36 022399055 37 022399056 38 022399057 39 022399058 40 022399059 41 022399060 42 022399061 43 022399062 4 i 022iii063 45 022399064 46 022399065 47 022399066 48 022399067 49 022399068 50 0223Y9069 51 022399070 52 022_ 399.0_ 7-1. 53 022399072 54 012399973 55 022399074 56 022399075' 57 022399076 58 0223Y9077 19 022399078 60 022399079 61 022399080 62 02239Y081 63 022399082 64 022399083 65 022399084 66 022399085 67 022399086 68 022399087 69 022399088 70 022399089 7 1 022399090 72 022399091 73 022399092 74 012.3.9.9.0~ 93.. 75 022399094 76 022399095 77 022399096 78 022399097 79 022399098 80 022399099 E 1 022399100 82 022399101 83 022399102 84 022399103 85 022399104 86 022399105 87 022399106 88 022399107 89 022399108 SWl.li8t T e w.011 Blank w.011 Blank ox 120, R B W ~ Z Z ~ - X ZaOik-1, 2/22/99 T o x lZ0, mL02229-RBtiver8lk-1, 2/22/99 Tox 120. mLO2229-5ppb-1, 2/22/99 Tox 120. RBLO2229-1oOpb-1, 2/22/99 Tox 120, RBLO2229-25ppb-1, 2122199 Tox 110, RBLOZ229-50-1, 2/22/99 Tox 110. RBLO2229-100ppb-1, 2/22/99 Tox 120, RBLO2229-25Oppb-1, 2/22/99 Tox 120. RBLO2229-500-1, 2/22/99 Tox 120. RBLO2229-75Ogpb-1, 2/22/99' Tox 120, UW2229-1OOOppb-1, 2/22/99 x.08 BluIk TOX 120 pio. 97T-01, 2/22/99 Tor 120 OLPO. 98.-01, 2/22/99 Tox 110 DM. 9 9 1 4 1 , 2/22/99 Tox 120 Dm. 1001-61. 2/22/90 TOX 120 Duo. 1011-61. 2/22/99 Tox 120, RBLO2229-250ppb-CCl. 2/22/99 moa a i d To* 120 m. 1021-01, 2/22/99 ' TOX 120 Duo. 1031-61. 2/22/99 Tox 120 OM). 1 0 4 1 4 1 . 2/22/99 Tox 120 pio. 1051-01. 2/22/99 Tox 120 o100. 106T-01, 2/22/99 TOX 120, RBW2229-250pp&cQ, 2/22/99 I(.oH B l M k T o x 120 pio. 1 0 7 1 4 1 . 2/22/99 "rex l20 m. 1 0 9 1 4 1 . 2/22/99. Tox 120 DNO. 1 1 0 1 4 1 . 2/22/99 Tox l20 m.111141. 2/22/99 Tox 120, RBL02229-250~b-CC3, 2/22/99 Blank Tox 120 W . 1 1 3 1 4 2 , 2/22/99 Tox 120 MD. 1 1 4 1 - e . 2/22/99 Tox UO DNO. 1 1 5 3 4 2 . 2/22/99 Tox 120 Dtx). 1 1 6 1 4 2 ; 2/22/99 -1 20 DNO. 1171-62. 2/22/99 Tux 120, R1)L02229-250ppb-CC4, 2/22/99 n8oB 81Mk Tor120 DNO. 1 1 8 1 4 2 . 2/22/99 Tox 120 DNO. 1191-01. 2/22/99 T o x l20 DM). l7.01-U. 2/22/99 T o x l20 DM). 12lr-02. 2/22/99 T o x l20 m.izzr-ca. ~ ~ 2 1 9 9 Toxl20. RBL02229-250opb-CC5, 2/22/99 nmoll a i h Tux 120 DM). 123.42. 2/22/99 Tux 120 DIIO. 1 2 5 1 4 2 , 2/22/99 Toxl20 PID. 126T-C2. 2/22/99 Tux 120 Du). U7T-Or; 2iZZf99 Tux 120 Dm. u 9 r - a . 2/22/99 M X 110, ~LO2219-150pob-CCI. 2112199 WSQg EIlMk -1 2 0 MD. 1301-03. 2/22/99 T o x l 20 QIO. 13Y-03. 2/22/99 a T o x l.20 Ip1D. 1 3 2 1 4 3 , 2/22/99 T o x l 0 QK). l 3 3 1 - 0 3 . 2/12/99 T o x l30 DNO. 1 3 4 1 4 3 . 2/22/99 ' T o x l.20 , RBL02229-25Oppb-CCl. 2/22/99 nmoll a l a - 1 2 0 Dm. 13S1-03, 2/21/99 T o x l2 0 END. 1361-03. 2/22/99 T o x lao DIPO. 1 3 7 1 - ~ 3 ; 1/22/99 T o x la!O DR). 1 3 8 1 4 3 . 2/22/99 T o x l.a10 DWQ. 1 3 9 1 4 3 . 2122199 T o x lLa!O, MLO2229-250ppb-rr8. moa allMk Toxl1.O Dm. 14lT-03.. 212219..9 T o x l.210 DIR). 1421-03. 2/22/99 TOX a.a10 DIIO. 1431-03. 2122199 2/22/99 T o x lLa10, RBLOZU9-25Oppb-CC9, 2/22/99 w.011 Il l u l k T o x l.2~ 0 , 1 0 6 r 4 i - ~ 5 0 p o b - x s , 0122/99 T a x 13.O. 1061-01-25Oppb-~D, 2/22/99 T o x la!O. I1BL02229-25Oppb-CClO. 2/22/99 1IlMk ' p o x 3310, RBLO2229-IIlO Blk-2, 2/29/99 Toxl.210, RBL02229-RBLzlnfllk-1, 2/22/99 T O X I210, RBLO2229-5ppb-2, 2/22/99 T o x la0. mI.02229-IOppb-2, 2/22/99 T a x a20. M L 0 2 2 2 9 - Z 5 ~ ~ b - 2 .2121199 Tox 120; mL02229-50&2; 1/22/99 Tox 120, RBL0222 -100*2, 2/22/99 Tox 120, U8'02228-2500pb-2, 2/22/99 TOX 120, RBLO2229-5OOppb-2. 2/22/99 Tox 120, RBLO2229-7500pb-2, 2/22/99 TOX UO. RBL02229-10OOppb-2, 2 / 2 2 / 9 9 = Alma 1.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 8.061 8.675 8.759 8.762 8.677 8.676 8.676 8.673 27 0.00 -100 bb 54 32.75 3 1 bb 70 55.49 11 m! 102 100.10 0 YSI 186 220.53 -l2 m! 374 489.80 -2 m! 567 765.59 2 m. 661 901.40 -10 250.00 8. 592 151 2SO.Oq 8.506 250.00 250.00 150.00 250.00 250.00 250.00 250.00 250.00 8.595 8.679 8.590 8.593 8.592 8.504 8.510 8. 591 8.593 8.592 8.594 8.507 8.500 8. 677 8.503 8.586 8.505 8. 505 8.593 a.m 8.503 8.503 8.593 8.593 CIS03 8. 594 8 . 502 8.504 8.506 8.505 8.505 8.594 8.503 8.507 8.507 8.592 8.483 8.482 5.00 ao.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 8.506 .8.503 8.506 8.505 8.499 8.460 174 J 187 221.85 -11- 1358 1898.79 1303 1818.80 111( 1295 1807.63 mf lZ05 1108 188 969 117 6 1259 963 1000 218 Y 93 944 1190 831 648 236 1341.15 1638.14 17S6.SS 1333.33 1386.13 267.30 1l330755:4981 1800.66 1144.26 882.27 291.90 IQ4 E\7 w( ' . MI bb 17 bb 752 1031.24 4so 598.82 733 1003.79 485 649.39 610 827.91 198 237.74 850 ,1171.20 504 676.47 651 886.33 566 764.35 545 735.28 229 282.01 13 5l3 689.15 366 477.87 371 485.66 1 0 1 241.83 -3 143 159.67 205 248.87 202 243.72 68 11.68 3101 117 121.65 22 m! 191 228.62 -9 mf .. 424 560.88 531 714.16 843 1161.85 1 2 3m -1 101 16 8 d . uat: c : \ # n ~ ~ \ r r c r - i . m o \ ~ i ~ \ o z n t g 9 a m t maifid: Ilu 1.b 25 13:24:27 1999 ,lutbDd: C:\YUsL~\1rcr-l.1110\14tM.\022399Q m t maifid: Ilu 1.b 25 13:25:14 1999 Job E o 6 1 hintdl Ilu 1.b 25 13:43:49 1999 i 1 1 3 7 1 I I i 1 -1 I1 I .I 1 I 51 MI13 # Sam 1022399020 2 022399021 3 022399022 4 022399013 5 022399024 6 022399025 7 022399026 8 022399027 9 022399028 10 022399029 11 022399030 12 022399031 13 022399032 14 022391033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399031 20 022399039 21 012399040 22. 022399041 23 022399042 24 022399043 25 oza399044 26 012399045 27 012399046 28 oza399047 29 012399048 30 012399049 31 022399050 32 012399051 . 3 3 022399052 34 022399053 35 02Zj99054 36 022399055 37 021399056 38 02i399057 39 022399058 40 022399059 41 021399060 42 022399061 43 022399062 44 022399063 45 022399064 46 022399065 47 022399066 48 022399067 49 022399068 50 022399069 51 022399070 52 022399071 53 022399072 54 922399073 55 022399074 56 021399075 57 022399076 58 022399077 59 022399078 60 022399079 61 022399080 62 022399081 63 022399082 64 022399083 65 022399084 66 022399085 67 011399086 68 022399087 69 oaz399088 70 022399089 71 022399090 70 02~399091 73 022399092 74 022399093 75 022399094 76 022399095 77 021399096 78 022399097 79 021399098 80 022399099 81 022399200 82 022399101 83 022399102 84 022399103 IS 022399104 86 022399105 17 012399106 88 021399107 89 022399108 1(.011 1IlA T a x l20, RllLo2229-iIZO Blk-1, 2/22/99 TOXIa0, RBLo2229-RllLiverBlk-1, 2/22/99 T a x l2 0, RllLO2229-5Opb.l. 2/22/99 T a x l.20, RllLO2229-10ppb-1, 2/21/99 T a x 1.20, RBLO1229-25-1. T a x la0. RBLO2229-50-1, 1/22/99 2/22/99 T o x l.20, RBLO2229-lOODpb-1, 2/22/99 T a x 1.20. mLo2229-25oQpb-1, 2/22/99 m x 1a0; mLO2229-5OOpob-1. 2/12/99 T a x 3.180, n~~oz~29-7509pb-ia.izzi99 T o x l30, mL02229-1000ppb-1, 2/22/99 Maon Iilank m x I.20 DWD. 971-01, 2/22/99 T a x 190 DWD. 98.41. 2/22/99 T O X l.20 Euo. 99141. 2/22/99 TOX I.ZO.DUO. ioor-ci. iiozio9 Tax 120 DWD. 10Y-c1. 2/22/99 Tax lZ0. RBLO2229-250-1.-- 2/22/99 mol! .I2I1ink TOXI 0 OK). 102141. 2/22/99 m x 190 OUO. 1031-61. 2/12/99 T a x la0 Euo. 104?-01, 2/22/99 T o x l.l0 Euo. 105141. 1/22/99 TTaaxx1l3300,DRR)B.Lo2120169?-4215.op2O/b2-2c/Q9.9 2/22/99 WCOB .I211ank Tax 1 01 UD. 107141. 1/21/99 Tox 1.20 DWD. 1091-01. 2/12/99 T a x 1.I0 OK). 110142. 2/22/99 Tox 1.20 wo. 1111-01. 1/22/99 T o x l.20 , m~oa229-~50ppb-cc3w,zzi9s XIOH .B2llulk T a x l 0 Dwo. 1131-GZ. 2/22/99 , T O X l.20 T o x l2.P 0 m.1141-02, DNO. 1151-02. 2/22/99 2/22/99 T o x l 0I DNO. 116?-GZ, 2/22/99 T a x 1.a 01 DNO. 1171-02, 2/22/99 T o x la01, RBL02229-250opb-CC4+ 2/22/99 MaoH '.aIlulk T o x l 0 DNO. 118F-62, 2/22/99 T o x l.20 m.1191-u, 2/22/99 T o x l.20 m.1201-u. 2/22/99 T o x la.I0 m.1211-a. 2/22/99 T O X I 0 wo. 122.-a, 1/12/99 T o x l30, ~Lo2229-25Oppb-CCS.2/22/99 WCOB allsdr T a x 1a0 Dwo. 1231-GZ. 2/22/99 T a x 13.a0 DNO. 1251-62, 1/22/99 T o x l 0 m.1261-02. 2/22/99 T o x l.a 0 m.ll71-01. 2/21/99 TOXI.a 0 cB1D. 1291-03, 2/22/99 T o x la0, RBLo2229-25Oppb-CC6. 2/22/99 NeOH ablank Tax U O DUO. 130143. 2/22/99 Tox U O DUO. 1311-03. 2/22/99 Tox 120 DI1D. 132143. 2/22/09 Tox UO Dwo. 133?-03, 2/21/99 Tax 110 Dgo. u4rk3. 2/12/99 . Tox 1 1 0 , ~2229-250ppb-crrr.2/22/99 y.oB Blank .mX uo uno. i m - 0 ~m z i w Tax 130 RQ. 1361-03. 2/22/99 Tax 120 Dgo. 1371-03. 2/22/99 TUX 120 Dwo. 1381-03. 2/22/99 Tax 120 m.139143. 2/22/99 TOX uo,m ~ ~ z ~ z 9 - r s o ~ b - czciaz.a199 I(.oB Blank Tox 120 DNO. 141143. 2/22/99 Tax 120 m.14ZF-C3+ 2/22/99 ToX ll0 MR. 1431-C3, 2/22/99 Tax uo, mLo2229-25oppb-CC9, 2/22/99 lloon Blulk ToX 120. 1061-01-25Oppb-MS. 2/11/99 Tnx l 2 0 , 106?-01-ZSDppb-118D, 2/22/99 Tox 120. mLO2229-2SOppb-CC10, 2/22/99 l40011 Blulk TOX 120. RllL02229-1110 Blk-2, 2/22/99 Tox 120. RBL02229-mLiwrIlk-1, 2/22199 m%120, RBLO2229-5pOb-2, 2/22/99 To% 120. MLO2229-10-2, 2/22/99 T U X 120, RBLo2229-25ppb-2, 2/22/99 Tox 120. llLO2229-5ODpb-2, 2/22/99 Tox 120. mLo3219-loop&-2, 1/22/99 TOX 120, RBLOl219-25Oppb-2, 2/22/99 TUX 120, RllL02229-500ppb-2r 2/11/99 TOX 120. R1ILOl219-75Oppb-2. 2/22/99 Tox 120, RllLO2229-1OOOppb-2, 2/22/99 i I BACK TO MAIN l t d EOM .ru 5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 9.634 9.632 9.549 9.550 9.549 9.549 9.546 48 139 266 609 lZ16 1665 2240 7.83 47.96 103.36 253.97 519.96 716.70 968.56 -69 bb -4 )oI 3 bb 2 bb 4 )oI -4 bbX -3 M. 250.00 9.803 9.465 45 6-83 )oI 463 250.00, 9.467 570 236.64 -5 bb 250.00 250.00 250.00 250.00 a5o.00 250.00 25o:oo 250.00 5.00 10.00 15.00 50.00 100.00 250.00 500.00 750.00 1000 .oo 9.467 9.552 9.463 9.466 9.465 9.462 9.383 9.464 9.466 9.464 9.466 '9.465 9.460 9.550 9.461 9.459 9.463 9.465 9.466 Y.456 9.377 9.462 9.465 9.466 9361 .466 9.375 9.462 9.464 9.464 9.377 9.467 9.376 9.380 9.382 9.463 9.355 9.355 9.441 9.455 9.380 9.377 9.379 9.376 9.380 9.379 9.372 9.418 537 m . 1 1 -11 bb 345 137.92 bb 310 111.59 bb 362 145.65 bb 287 1U.50 bb . 301 118.70 bb 552 218.82 -8 bb 2 268 1054.9365 272 a25 85.54 bb 265 102.84 'bb 182 66.50 bb 581 241.64 -3 10I . 216 254 . 98.31 3 bb 290 113.87 *! W 239 91.83 bb 81 a . 2 5 . IO4 641 268.03 7 bb 105 32.90 bb 91 26.88 bb 100 30.68 bb 41 4-75 bb 175 63.73 bb 540 223.53 -11101 . 89 13.71 bb 62 zii7i bb 74 19.55 bb 113 36.63 145 50.38 555 230.10 101 bb -8 101 130 43.91 124 41.04 8L 23.72 444 181.67 572 037.76 518 213.77 5lS 212.78 39 3.88 49 11 91 164 288 580 1113 1704 2676 6-57 1.01 29.94 58.91 113.11 241.07 479.30 733i58 1lS9.74 71 bb -90 mI 20 mi 18 mi 13 )oI -4 bb -4 101 -2 IMX 16 WK I '1I fE.&X& O%]'Lbh I BACK TO MAIN i 1 I i' I I I 1 1 1 1 I I 1 i i caV0Uad 6 : X I 01022399020 2022399021 3 022399022 4 022399023 5 022399024 6 022399025 7 022390026 8 022399027 9 022399028 10 022399029 11 022399030 12 022399031 13 022399032 14 022399033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399018 20 022399039 21 052399040 22 022399041 23 022399042 24 022399013 25 022399044 26 022399045 27 022199046 28 022399047 29 022399048 30 022399049 31 022399050 32 022399051 33 022399052 34 022399053 35 022399054 36 022399055 37 022399056 38 022399057 39 022399058 40 022399059 41 022399060 42 022399061 43 022399062 44 022399063 45 022399064 46 022399065 41.022399066 48 022399067 49 022399068 50 022399069 51 022399070 52 022399071 53 02a399072 54 022399013 55 022399074 56 022399015 57 022399076 58 022399017 59 022399078 60 022399079 61 022399080 62 022399081 63 022399082 64 022399083 65 022399084 66 022399085 67 022399086 68 0223990ai 69 022399088 1 0 022399089 71 022199090 72 022399091 73 022399092 74 012399093 15 02139909i 76 021399095 77 012_ 399.09~ 6 1 8 022399097 79 022399098 80 022399099 81 022399100 82 022399101 83 022399102 84 022399103 85 0223bgioi 86 022399105 87 022399106 88 022399107 89 021399108 I..pldbt T m HaOH 8l.nlr 11.011 Blank Tox 120. BBL02229-HZO Blk-1, 2/22/99 TOX 120. IU)L02229-RBLl4~Blk-l,2/22/99 TOX 120. RBLO2229-Sppb-1, 2/22/99 TOX 120. RBLO2229-lOgpb-1, 2/22/99 'PO% U O . RILO2229-25-1, 2/22/99 Tox 120. RBM2229-50Wb-1. 2/22/99 Tox 120. RBL02229-100Wb-1, 2/22/99 ' 'POX 120, RBW2229-25OWb-1. 2/22/99 Tax 120; RBLO2229-500ppb-1, 2/22/9,9 'pox 120. BBL02229-75OWb-1, 2/22/99 TOX 120. RBLO2229-1OOOppb-1, 2/22/99 w x Blank Tox U O DIO. 9 7 1 4 , 2/22/99 z / w w TOX 120 0)~). 981-u, 2122199 TWC 120 PR).99r-01. TOX 120 DIO. ioor-Ei. 2122199 Tox 1 2 0 D!SO. 101?41. 2/22/99 Tox 120. R B L 0 2 2 2 9 -..2 5 0 H L 2/22/99 IWM BlAnk TOX ilo-DtPO. io2r-~i,w w 9 9 TOX 120 DNO. 103?-01, 2/22/99 TO% 120 DR). 1011-01. 2/22/99 TOX 120 DNO. 105?-01. 2/22/99 Tox 120 CM). 106C41, 2/22/99 . M x 120, RBt02229-2SOppb-CC2, 2/22/99 XOOH Blank Tox 120 DM). 107?41. 2/22/99 Tux 120 DM). 109141. 2/22/99 Tox 120 DNO. 110?41, 2/22/99 To* 120 DM). 111.41. 2/22/99 Tox 120. RBM2229-2500pb-CC3, 2/22/99 1IIOH Blu* Tax 120 DNO. 113?-61, 2/22/99 Tox 120 m. 114142, 2/22/99 Tox 120 DNO. 115?4. 2/22/99 'POX 120 DNO. 116?-02, 2/22/99 Tox 120 m. 111?4, 2/22/99 TOX 120, RBLO2229-25oppb-rr4, 2/22/99 Y.OB Blank M X 120 m.118?4, 2/22/99 ax 120 DNO. iig~-~z.o/a2/99 Tax 120 m. 12or-u; 2/22/99 Tox l z o Dwo. 1211-G2, 2/22/99 Tox 120 DHO. 122?4. 2/22/99 Tox 120, RBL02229-250p&CS, 2/22/99 HaOR B l h Tox 120 Duo. 1233-61. 2/22/99 TOX 120 DNO. 1 2 ~ 4 z,izzi99 TOx 120 Duo. 1161-61, 2/22/99 To% 120 DNO. 127?4, 2122199 TOX IZO DND. imr-03. ziz2199 Tax 120, RBLO2229-250pplrcc6. 2/22/99 11.08 n i u a Tox ll0 Dwo. 130143. 2/22/99 TOx 120 DM). 131F43, 2/22/99 Tox 110 DND. 132?-03. 2/22/99 TOx l20 DNO. 133?-C3; 2f22199 Tox 120 Duo. 1341-03, 2/22/99 ' TO% 120, RBL02229-250-. 2/12/99 Tox U O . RBL02229-2SQppb-CC8. 2/22/99 lwlB Bl4nL T U 120 Duo. 14lT-03. 2/22/99 Tok 120 Dwo. 142?43: 2/22/99 TOX 120 m. 143r-63, 2 1 2 ~ 9 9 To% 120, RBLO2229-250ppb-CC9, 2/22/99 NOR. ni.nL Tox 120, 1061-01-250pob-xII,2/22/99 T W 120. 106?-01-25~-1UD.2/22/99 ~ Tox~ 120.; .mLo2zz9-asOopb-ccio. zizzigg MaOR Blu%k To% 120, RBLO2229-mO Blk-2, 2/21/99 TOX 120. RBLO2229-RBLiverBlk-1, 2/21/99 'Tax 120. RBL02229-5mb-2, 2/22/99 Tax 120. RBLO2229-1Oppb-2, 2/22/99 Tox 120, RBL02229-25pp&2, 2/22/99 Tox 120, RBLO2229-SOW-2, 2/22/99 Tox 120, RI)L02229-100ppb-2,2/22/99 Tox 120. RBLO2229-25Oppb-2, 2/22/99 Tox 120. RBLO2229-500ppb-2, 2/22/99 Tox 120, RBt02229-750ppb-2, 2/22/99 Tox 120, RBLO2229-100Oppb-2, 2/22/99 .oyO 020199tr auurot1tmt.d and srlntd m a 2/2S/99 2so.00 250.00 250.00 250.00 150.00 150.00 250.00 250.00 250.00 7.494 7.492 7.493 7.485 7.492 7.490 7.493 7.493 7.493 7.408 7.489 250.00 7.577 7.492 7.321 7.323 7.3ia 7;324 . 250.00, 7.495 7 .I22 7.323 7.237 7.325 7.237 250.00 7.495 7.325 7.227 7.234 7.326 150.00 7.410 7.321 7.239 7.323 7.237 7.- 2SO.00 250.00 250.00 250,oo 250.00 250.00 250.00 250.00 250.00 250.00 250.00 250.00 250.00 250.00 7.408 7.234 7.317 7.236 7.231 7.324 7.313 7.234 7.233 7.323 7439 7.234 7.321 7.233 7.320 7.237 7.236 7.236 7.325 7.233 7.131 7.237 7.323 7.l28 7.U7 7.306 7.282 7.314 7.238 7.235 7.237 7.234 7.238 7.235 7.233 7.196 .zu 224 72.66 -71 Y( 298 96.74 -61 101 680 220.47 -12 W 746 141.86 -3 w 796 258.32 3 W 853 276.63 11 101 794 257.65 3 W 760 246.58 718 252.33 -1 101 1 w 762 247.11 -1 W 796 258.26 3 101 695 9n 9 89 923 ,1010 990 225.34 316.98 320.94 299.48 327.67 321.69 -10 1(y 27 W 28 101 20 w 31 W 26 101 366 118.81 -52 IOI 853 zi6.70 ii Wn 879 285.02 933 302.56 14 101 21 a 879 285.03 14 101 731 237609 -5 IIy 382 813 759 1095 860 123.76 263.69 246.30 355.07 278.88 -SO IU 5 IU -1 101 42 IU 12 10( 402 130.48 -48 kQ4 858 278.28 764 ar1.95 1-~1 10( -1 k ~ 4 851 276.07 827 268.41 10 IU 7 a 750 243.43 -3 bb 389 l26.22 -50 1Iy 757 245.70 -2 IOI 648 210.04 -16 101 7413 240.02 -4 lQI 690 223.71 -11 bb 13 4 270.53 8 IU 380 123.14 .$illQI 80 6 261.33 p 5 101 956 310.11 "24101 859 278.55 11 BD! 768 249.26 0 101 739 239.81 -4 lQI 39s 108.24 -49 bb 633 ao5.43 -11 ~QI 869 282.03 13 1oL 801 259.89 4 W 855 217.34 11 101 620 101.13 -20 BD! 353 .114.56 -54 101 736 238.63 -5 101 452 146.77 -41 101 578 187.41 -25 101 646 209.58 -16 101 176 251.73 1101 343 121014.4405 --5158 1b0b( 630 707 229.40 -8 101 701 227.53 -9 101 305 99.05 -60 PI 567 183.95 -26 IU 776 251.81 1101 312 120.61 -52 W 291 94.34 -62 101 765 248.04 -1 819 26f.74 6 IPI 793 357.34 3 ~DI 814 263.97 750 243.20 6 101 -3 IOI . 791 256.51 3 W 645 209.07 -16 10( 762 247.20 710 249.83 -1 IOI 0 ma BACK TO MAIN i i I !ompound1 name:PFOS lorrelation coefficient:r = 0.999604;P2=0.999208 . lalibration curve: 28.4213 * x + 269.890 1 .esponsetype: ExternalStd,Area lurve type:Linear,Origin: Exclude, Weighting: Null, Axis trans: None 1 3.12e4 3 3 1' 1 I 1 Response _i 1 I 1 1 1 l " " l""l 200.0 " " l ' " '40I 0'.0" ' I " " ~ " 60"0.~0 I - 1- Y. mow oaoiss:: gluocit.td'.lrd oJint.a a~ar~ss I I i .. . n . .* _: 800.0 . . I I icon( 1ocIo.o 1 I 7 :ompound 2 name: PFHS :orrelation coefficient:r =0.998267, fl2 = 0.996537 . M.ibration curve: 21.3424 * x + 163.104' 1 lesponse type: E x t e d Std,Area h e type: Linear, Origin:Exclude, Weight&: Null, Axis trans: None 1 2.15e4 3 1 1' 1 i f Response 1 1 1 I I 1 1 0 I I f :\ t .. BACK TO MAIN . ._ Paw 2 1 1 3 lompound 3 name: POAA :omlation coefficient: r = 0.998245, i^2 = 0.996493 . :&bration curve: 3.35408 * x + 33.1971 1 lesponse type: External Std, Area : m e type: Linear, Origin:Exclude, Weighting: Null, Axis trans: None 1 3.87e3 3 '1 1' I 1 .I Response _1 :I 1 I I 1 I 0 I i ! :. BACK TO MAIN .. BACK TO MAIN ~ lompound 4 name: M513 lorrelation coefficient: r =0.995618,ih2= 0.991256 Mibration cwe: 0.698781 * x +315781 Lesponse type:External Std, Area lurve type:Linear, Origin:Exclude, Weighting: Null,A x i s trans: None 843 .. .. .. Response 1 1 1 orrilationcoefficient:r =0.997791, +2= 0.995587 . &bration curve: 2.28 142 * x + 29.8900 1 esponse type: External Std, Area w e type: Linear, Origin:Exclude, Weighting:Null, Axis trans: None 1 2.68e3 3 1 rl' 1 I 1 i i Response 1 1 I I 1 ! 1 1 i 1 " " "20"0.I 0" " I " " I " 4"0l0."0 " l ' " ' ~ " 600.0 BACK TO MAIN .Uh .? . 800.0 I , I I ~Conc 1000.0 i I i i I eGonse type: External sta, Area 1 lurve type: RF 1 853 3 1 1' I I I Response 1 1 1 I I 1 f 100.0 200.0 1 1 " " l ' 5"0".0 I""[ roup O a O ~ S s : : v t i t a t . 6 .9d print.6 by Y a/as/ss ''IL --.- ~ ' "15'0.0~ " " ~ " ' I 1 ! BACK TO MAIN . -_ X X . . I , I ~Conc 25o:o 1 i i 1 1 1 1 I T 1 .I I 8 ; mlatiw bI*tl &molutmb i g h t r i Relative UUI Abmolut. UUI 1 -1 3 1 1 1 I i ??Va t-1 TIC A i.oooooo. 1.000000 a.ooo mvbid 0.016. o.ooo. mIM1a.dd 1.030 YUUt 10s 30.00 ' 70.00' 4.00 . 10.00 - . Divbld lo.00 so.00. -14 0.00, meabld 3.00. W e d , 0.00. D i u b l e d - I .. BACK TO MAIN . -_ BACK TO MAIN SOUP 020199: FACT Equipment Log 99014 Quattro 11, #I64143 PAGE .. DATE: 2/24/99 *. ANALYST: drb STUDYNUMBER:Tox-120 MATRIX: Rat J.iver DESCRIPTION Liver SamDles I . FACTMethock FACT-M40, . Injection +olume: 10 uI. Cycle time: 13.5 nhin, Electrode: 7Fsurav Guardcolumn: None Part number: na Lot number: na Analytical column: Kevstone Betasil size:2x100mm Type: C 18 Sphere size: 5 yn I t Part number: 105-701-2 Lot numb& P8C09 . . Serialnumbet110881106D 9 . Analytical parameters: ESI nebulizing gas (Lh) 15 Lc Flow rate; 300Wdn, . Shea& gas (I,@ 0 * ) e HPLCsolvent gradient: Long 2 .. 96B 1) 0.00 40.0 . 2) 850 90.0 3) 11.0 90.0 4) 12.0 40.0 5) 13.5 StOD - - .. . -- m i n g Method Report Method: c:\MA~~L~M~\FAcT-~.P~o\A~Q~DB\PBOS Printed: Wed Feb 24 15:22:59 1999 . SWb b301sq 1Ac OSllblSS .. . ...: , . .. BACK TO. . MAIN .._._. Page 1 * ' WS SOURCE ( ESP- ) Caglllary . "Cone Extractor RF Lens Source Block T q p . Desolvation Temp. Set Rdbk 3.00 60 5 0.2 150 ,250 2.99 -60 -7 .150 250 Pro 6 sure s I1 . Falyser Vacuum Gas Cell . t- : Rdbk 1.2e-4 5.2e-3 ' LM Resolution HM Resolution Ion Energy Ion Energy Ramp ' Lens 6 Multiplier . :\ w2 LM' Resolution HM Resolution Collision Ion Energy Ion Energy Ramg Lens 8 Lens 9 ' Multiplier Set Rdbk 14.0 14.0.4 2.0 i . 0.0 5 3 650 set Rdbk 15.0 15.0 * 45 ' 42 2.0 0.0 40 38 0 .42 .650 ! i 1 i 1 1 I g -1 1 399.00 80.00 0.2s 4s 2 399.00 99.00 0.01 45 3 399.00 130.00 0.25 , 45 1 1 ! ! 1 I Mion I3 IRY of a YUI irin ( rur- 1 Intu c h u w l hlay ( S e a 1 : Smn ( D I ~ ~ O N) x Start T i m ( Xixu 1 : nd T h ( UN 1.1 U0Pm.t. X Qunrul Paraat Daughtu 0.03 0.00 0.00 13.50 1 -11 . (Smcal Coll 1 299.00 80.00 0.25, 2 299.00 99.00 0.15 4s . 4s hlaotion 1 4 m of 3 YU. ?air.'( Intu c h u w l hlay ( Soca I x 8P.a ( m k 0 N 1 X . Stut Tfnv t H i x u x * 0.03 0.00 0.00 rm- I 1 4l3.00 119.00 0.25 2 413.00 m . 6 0 0.21 3 ru.00 ais.00 0.15 30 . 20 30 60 60 60 (.VI C o r u C V 1 l!l 1s u 3 1 1 . 1 S69.00 169.00 . 0.2s 4s 2 S69.00 219.00 0.2s 4s 3 sc9.00 119.00 o.as 4s I J I 1 427.00 80.00 0.21 40 i 35 35 3s 60 -_e- BACK TO MAIN .. r $. :\ -- BACK TO.. MAIN - u_ I - Masslynx Sample List I Sample List:CAMASSLYNWACT-1.PRO\SarnpleDB\022499a.SPL 1 In Printed Wed Feb 24 15:25:39 1999 0199 IAc-cr//0 Page 1 File Name File Text nkS File Inlet1 Bottlc olume Samplen ConcA Conc B ConcC 1 1 022499001 MeOHBlank TOX12C long2 91 2 022499002 MeOHBlank TOXl2C long2 91 3 022499003 TOX120, RBL02229-H20 Blk-l,2/22 TOXl2C l o n g 1 4 022499004 Tox 120, RBL02229-RBUverBlk-1,2 TOXl2C long2 2 1 5 022499005 Tox 120, RBL02229-5ppb-1,2/22/99 TOX12C long2 3 6 022499006 TOX120, RBL02229-1Oppb-l,2/22/9 TOXl2C long2 4 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Sfandard 10 standard -1 7 022499007 Tox 120, RBLO222425ppb-l,2/22/9 TOXl2C long2 5 . 8 9 022499008 Tox 120, RBL02229-50ppb-l,2/22/9 022499009 Tox 120, RBLO2229-100ppb-l,2/22/ TOXl2C .long2 TOX12C long2 6 7 . 10 022499010 Tox120,RBLO2229-250ppb-1,2/22/ TOX12C long2 8 11 022499011 Tox 120, RBL02224500ppb-1,2/22/ TOX12C long2 9 12 022499012 Tox 120, RBL02229-750ppb-1,2/22/ TOX12C long2 10 13 022499013 TOX120, RBL02229-1000ppb-1,2/22 TOXl2C long2 11 14 022499014 MeOH Blank TOX12C long2 92 15 022499015 Tox 120 DNO. 145F-G4,2/22/99 TOXl2C long2 12 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Analyte 10 Analyte. . 16 022499016 'Tox 120 DNO. 146F-G4,2/22/99 17 022499017 Tox 120 DNO. 147F-G4,2/22/99 TOXl2C long2 13 TOXl2C long2 14 f 18 022499018 Tox120DNO. 148F-G4,2/22/99 TOXl2C long2 15 19 022499019 Tox 120 DNO. 149F-G4,2/22/99 TOXl2C long2 16 20 022499020 Tox 120, RBL02229-250ppb-CC11, : TOXl2C long2 17 21 022499021 MeOH Blank TOXl2C long2 92 1 22 022499022 TOX120 DNO. 150F-G4,2/22/99 TOXl2C long2 18 23 022499023 Tox 120 DNO. 151F-G4,2/22/99 TOXl2C long2 19 24 022499024 Tox 120 DNO. 152F-G4,2/22/99 TOXl2C long2 20 25 022499025 Tox 120 DNO. 153F-G4,2/22/99 TOXl2C long2 21 022499026 T0x 120 DNO. 154F-64, 2/22/99 Toxi2c tong2 22 i 2267 022499027 Tox 120, RBL02229-250ppb-CC12, : TOXl2C long2 23 28 022499028 MeOH Blank TOXl2C long2 92 29 022499029 Tox 120 DNO. 155F-G4,2/22/99 TOX12C long2 24 30 022499030 Tox 120 DNO. 157F-G4,2/22/99 TOXl2C long2 25 1 31 022499031 Tox 120 DNO. 158F-G4,2/22/99 TOXl2C long2 27 32 "022499032 Tox 120 DNO. 159F-G4;2/22/99 TOX12C long2 28 33 022499033 Tox 120, RBL02229-250ppb-CC13, : TOXl2C long2 29 022499034 MeOHBlank TOXl2C long2 93 1 3354 022499035 Tox 120 DNO. 161F-G5,2/22/99 TOXl2C long2 30 36 022499036 TOX 120DNO.162F-G5,2/22/99 TOXl2C long2 31 37 38 ] 3490 41 42 43 1 44 45 46 47 1 '48 49 50 51 I . 52 53 54 55 56 1 57 58 59 022499037 Tox 120 DNO. 163F-G5,2/22/99 TOX12C long2 32 022499038 Tox 120 DNO. 164F-G5,2/22/99 TOXl2C long2 33 022499039 Tox120DNO. 165F-G5,2/22/99 TOXl2C long2 34 022499040 Tox 120, RBL02229-250ppb-CC14, : TOXl2C long2 35 022499041 MeOHBlank TOX12C long2 93 022499042 Tox 120DNO. 166F-G5,2/22/99 TOXl2C long2 36 022499043 Tox.120 DNO. 167F-G5,2/22/99 TOXl2C long2 37 022499044 Tox120DNO.168F-G5,2&2/99. TOX12C long2 38 022499045 Tox 120 DNO. 169F-G5,2/22/99 TOX12C long2 39 022499046 Tox 120 DNO. 17OF-05 2/22/99 TOX12C long2 40 022499047 Tox 120, RBL02229-250ppb-CC15, : TOX12C long2 41 022499048 MeOHBlank TOX12C long2 93 022499049 TOX 120DNO. 171F-G5 2/22/99 TOXl2C long2 42 022499050 Tox 120DNO. 173F-G52/22/99 TOXl2C long2 43 02!2499051 Tox 120DNO. 174F-G52/22/99 TOXl2C long2 44 022499052 Tox 120 DNO. 175F-05 2/22/99 TOX12C long2 45 022499053 Tox 120 DNO. 177F-G6 2/22/99 TOX12C long2 46 022499054 TOX120, RBL02229-250ppb-CC16, i TOXlPC long2 47 022499055 MeOH Blank TOXl2C l o n g 94 022499056 TOX120DNO. 178F-G6 2/22199 TOXl2C long2 48 022499057 TOX120DNO. 179F-G62/22/99 TOX12C long2 49 022499058 Tox 120 DNO. 180F-G6 2/22199 TOXl2C long2 50 022499059 ToX 120 DNO. 181F-G6 2/22/99 TOXl2C long2 51 .II .#.-.>.=- ' 10 Analyte 10 Analyte ' 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte . l o Analyte 10 Analyte 10 Analyte 10 Qc OI ' Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte i o Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte f , 1 - Masslynx Sample Llst - - *-- Sample List:CWASSLYNWACT-1.PRO\SampleDBV)22499a.SPL Printed Wed Feb24 15:25:39 1999 SOUPb20199. LAc 0 5 /LO!$? BACK TO MAIN ..- ..-. .Page 2 File Name File Text MS Flle Inlet I Bottlc olume SampleT] ConcA Cone B ss 60 -499060 Tox 120DNO. 182F-G62/22/99 TOX12C long2 52 10 Analyte 61 022499061 Tox 120, RBL02229-250ppb-CC17,: TOXl2C long2 53 10 QC 62 022499062 MeOH Blank TOXl2C long2 94 . ' 10 Analyre 63 022499063 Tox 120DNO. 183F46 2/22/99 TOXl2C long2 54 -10 Analyte 64 022499064 TOX120DNO. 184F-G62/22/99 TOXl2C long2 55 10 Analyte 65 022499065 Tox 120 DNO. 185F46 2/22/99 TOXl2C long2 56 10 Analyte' 66 022499066 Tox 120 DNO. 186546 2/22/99 TOX12C long2 57 10 Analyte 67 022499067 Tox 120DNO. 187F-G62/22/99 TOX12C l o n g 58 10 Analyte 68 022499068 Tox 120, RBL02224250ppbCCl8, : TOX12C long2 59. 10 Qc 69 022499069 MeOHBlank ' . TOXl2C long2 95 10 Analyte 70 022499070 TOXt20 DNO. 189F-G62/22/99 TOXl2C long2 '60 10 Analyte 71 022499071 Tox 120DNO. 190F462/22/99 TOXl2C long2 61 10 Analvte 72 022499072 Tox 120DNO. 191F-G6 2/22/99 TOXl2C long2 62 73 022499073 Tox 120, RBL02229-250ppb-CC1,U' TOX12C long2 63 74 022499074 MeOH Blank TOX12C long2 95 10 10 Analyte. . 75 022499075 Tax 120,110F-G1-250ppb-MS, 2/22r TOX12C long2 64 . 10 Analyte 76 022499076 Tox 120,ll OF-G1-250ppb-MSD, 2/2 TOX12C long2 10 Analyte 77 022499077 Tox 120, RBL02229-250ppb-CC2,2/ TOX126 long2 66 * 10 Analyte 78 022499078 MeOH Blank TOXl2C long2 95 10 Analyte 79 022499079 TOX120, RBL02224H20 BIk-2,2/22 TOXl2C long2 67 10 Analyte 80 022499080 Tox 120, RBL02229-RBtiverBlk-1,a TOXl2C long2 68 10. Analyte 81 022499081 Tox 120, RBL022295ppb-2,2/22/99 TOX12C long2 69 10 Standard 82 022499082 Tox 120, RBL02229-1Oppb2,2!22B TOXl2C long2- 70 10 Standard 83 022499083 Tox 120, RBL02229-25ppb-2,2/22/9 TOX12C long2 71 10 Standard 84 022499084 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72 10 Standard 85 022499085 Tox 120, RBL02229-1OOppb-2,2/22/ TOX12C long2 73 10 Standard 86 022499086. Tox 120, RBL02229-250ppb-2,W TOXl2C long2 74 10 Standard 87 022499087 Tox 120, RBL02229-500ppb-2,2/22/ TOX12C long2 75 10 Standard 88 022499088 Tox 120, RBL02229-750ppb-2,2/22/ TOXl2C l o n g 76- 10 Standard 89 022499089 Tox 120, RBL02229-1000ppb-2,2/22 TOXl2C long2 77 10 standard ConcC I 1 1 I -j Dilutions? Analyts 1: FACT: DATA REVIEW S - m YFORM BACK TO MAIN -... I I 13 L L .. - - .I BACK TO MAIN . 1 2 022499002 3 022499003 4 022499004 5 022499001 6 022499006 7 022499007 8 022499008 9 022499009 10 012499010 11 022499011 If 022499011 13 022499013 14 022499014 15 022499011 16 022499016 17 022499017 18 021499018 19 022499019 20 022499020 21 022499021 22 022499022 23 022499023 24 022499024 25 022499015 26 022499026 27 022499027 28 022499028 29 022499029 30 022499030 31 022499031 31 022499032 33 022499033 34, 022499034 35, 022499031 36I 022499036 37 022499037 38 022499038 39 022499039 40 022499040 41 022499041 42 022499042 43 022499043 44 022499044 45 022499045 46 022499046 47 022499047 48 022499048 4Y 022499049 SO 022499050 51 022499052 52 022499052 3 naoH Blank Tax 120. RBL02229-w10 Blk-1. 2122199 Tox 120; RBLO2229-RBLfverSli-1, 2122199 TOX 120. RBLO2229-5ppb-1, 2l22199 Tax 120. ULO2229-10-1. 2122199 Tox 120; 2122I99 Tox 120. 2122199 Tox 120, RBLO2229-loo&-1. 2122199 Tox 120. FBLO2229-ZSOppb-1, 2122199 Tox 1 1 0 , RBLO2229-SOOppb-1, 2122199. Tox l l 0 , RBLO2229-7SOppb-1, 2122199 Tox 1 1 0 , llBLO2229-1OOOgpb-1, 2/21/99 . XeOlI Ill& Tox 110 DDIO. 1 4 5 & 4 , 2/21/99 Tor L10 D100. 146?44, 2122199 Tox 120 CUO. 1 4 7 1 4 4 . 2122199 To% 120 CLEO. 148?&; 2l22/9i Tox 120 m.149?-64, 2122199 m. Tox 120. ML02229-250ppb-cQ1. x.oB Blank- Tox 120 l S 0 ? 4 . PI22199 TOX 120 DNO. 151?44; 2122199 1/22/99. TOX 130 DUO- 152T-04, 2l?2l99 Tox 110 QIO. l S 3 T 4 . 2122199 TOX Tox 112200, D~wo2.2 2l594-?-2C54o. p o21b2-2c1c9l92~2,122I99 w.08 Blank Tox 120 Iwo. 155?-01, 2122199 TO* 130 DNO. 1 5 7 1 4 4 . 2122199 TOX 120 m.1581-04. a m i 9 9 TOX iao DNO. IS~T-GI, z m i 9 9 T a 120, RBLO2229-250ppb-CC13 , 2122199 X.OK B l W TOX iao DNO. i s m - c s , 21~2199 'pox 1 2 0 DNO. 162?-05, 2127199 TOX 120 DNO. lSS?-CS, 2122l99 Tax 120 DNO. 164?-E5, 2122199 Tox 120 m. 165?-05. 2122199 *. mor3 Blank Tox 110 W O . 171?-05 2122199 ' Tox 120 173?4S 212219Y Tox l20 W O . 1-7 4.?.- G_I_ 2_ /_1-2-/.9.9 Tox f i 0 Duo. 1751-05 2122199 Toxl 56 02A499056. Tox 1 1 0 also. 17W-66 2122199 57 022499057 Tox 1JO W. 1 7 9 1 4 6 2121199 58 022499058 Trm l 2 0 DPID. 1801-C6 2122199 59 022499059 60 022499060 61 022409061 62 022499062 63 022499063 64 022499064 61 022499065 66 022499066 67 022499067 68 022499068 69 022499069 Tox 1 IO & . . l a 14?-G6 2122199 TOX 1 11~0.i aI S ? 4 6 2112199 Tox 1 :0 Owe. l a16?46 2122199 Tox 1 :010110. 1 I17)-06 2122199 Tox 1 0. RBLO2129-25OQpb-Ccl8, 1(.oH llank 2122l99 70 022499070 71 022499071 72 022499072 73 022499073 74 022499074 Tox 1 0 DUO. 1 89 r - c ~a m i 9 9 Tor 1 0 DUO. 1 910?-06 2122199 Tox 1 0 DWO. 1 91ii-c~ziazi99 Tox 1200 RBLO2229-2SOppb-CCl, 2122199 JhOH Blank 7S 022499075 TUX 120. 110?-~1-2SOppb-~,2122199 76 022499076 Tox B O , 11~?41-2SOppb-KOD. 2122199 77 022499077 78 022499078 79 022499079 80 022409080 Tox l20, rSLO2229-2SO~CC2. 2122199 mon n i b TUX 120, RBL02229-w10 elk-2. 2122199 ox 120. R B L 0 2 2 2 9 - W w r ~ - l , 2/21/99 81 022499081 TOX 120, XBLO2229-5ppb-1, 2/22/99 82 022499082 Tox 120. RBLO2229-10ppb-2, 2122199 83 022499083 Tox 120,.LI)LO2229-25ppb-2. 2122199 84 022499084 Tox 120, RBLO2229-SOppb-2, 2122199 85 021499085 Tox 120, RBL02229-100ppb-2, 2l22199 86 072499086 Tox 120. RIrL02229-2SOppb-2, 2122199 87 022499087 TOX 120, RBL02229-500ppb-2, 2122199 88 0 ~ 2 4 9 9 0 8 8 TOX 120, ~ ~ 0 ~ 2 ~ 9 - 7 s o p panba-1~99, 89 022499089 TOX 120, RBLO2229-1OOOppb-2. 2l22l99 qc M8lYt. Itd m 7.951 5.99 12.00 .19.90 59.98 110 00 299.00 599.00 898.00 1198.00 7.941 8.031 7.960 7.952 7.951 7.986 7.912 7.922 7.923 7.863 7.854 299.00 7.954 7.843 7.852 7.766 7.822 7.854 299.00. 7.958 7.797 7.863 7.159 7.829 7.157 299.00 7.927 7.860 7.840 7.856 7.869 299.60 7.926 7.846 7.858 7. rss 7.868 .7.836 199.00 7.937 7.869 7.843 7.813 7.858 7.838 099.00 299.00 299.00 299.00 S.9Y 11.00 29.90 39.90 120.00 299.00 599.00 898.00. 1191.00 7,947 7.825 7.868 7.854 7 . 750 7.161 7.869 7.921 7.849 7.856 7.752 7.176 7 . 854 7.927 7.931 7.863 7.853 7.849 7.861 7.861 7.931 7.937 7.862 7.788 7.871 7.867 7.696 7.782 7.848 7..9.47 ~ 7.918 7.990 7.081 7.911 7.884 7.899 7.852 7.869 7.868 7.861 7.866 91 418 961 642 1002 16x4 2898 7377 irJoi 2U41 27547 - 3698 302) 2429 / 3388 273s 6536 .. coaa. n w 1 0.00 101 - 1 0.00 101 1 5.18 101 1 28.41 W 1 14.74 W 1 30.18 101 56.29 101 l11.40 101 303.15 101 599.79 101 922.73 101 1 1167.U 1pI 1 145.67 1111 116.29 101 91;32 bb 132.39 2709 1 103.27 1111 3855 1 ls2.38 1111 2880 1 llO.60 1111 210s 1 77-44 1111 3459 1 135.41 1111 587% 238.88 1111 1975 4075 2570 4069 6615 71.84 101 161.10 101 97.34 101 161.56 101 270.57 101 2634 1126 2079 3031 2Ol3 6827 1 100.10 101 1 65.48 101 1 76.32 1111 l17.09 bb 73.46 101 279.66 101 2015 3075 2398 1835 1771 7326 73.58 1111 118.98 lQI 89.96 111 65.85 )(lt 63.U 101 301.06 101 lJ a7 16ll 1421 3019 2815804 0723 672 2500911 2888951 2788001 3070609 2923061 10931 l3 17 2658942 2786810 1179533 2500338 2181490 ~~ 9x2 1x49 17278SS 21276Y4 2116368 l~ o ~ l.l 8.. 132 6 13377 15270 1786 418 368 416 603 63 6 1180 1924 3319 7281 1 3 610 20328 2,9544 1 42.37 101 1 16.26 101 1 4 8 . l s 1111 -1 116.58 lQI 1 110593.. BW 1 360.07 1 16.03 111 1 101105.. IOI 1 133736.. BW .1 ll9402.. 101 1 131506.. PI 1 sS187.'. II x. 455.64 1111 1 43.6s m 1 iu8jrII PC 1 ll9351.. PI 93310.52 101 107081.. 101 93424.33 I 386.U 101 31.60 101 73994.38 I W 0 . 1 7 101 92348.31 101 422.36 1111 52.63 I 560.za bb 1 641.32 bb 1 231.07 I 1 5.17 101 1 3.01 bb 1 5.10 bb 1 l3.08 Mac 1 14.50 bbX 1 37.01 101 1 69.67 #( 1 119.40 am 1 299.u m 1 570.21 LpI '1 857.94 rn 1 1252.68 101 374 23 1 -6 -7 1 0 3 -3 118 21 26 16 8 0 -5 -4 1 Ioup 020199: Quantitated on 03/091S .M printmd on 03liOl99 W I I BACK TO MAIN Quantify colpouod 8um.w R o D o r t mx-120 rut L i v u #-lo List: C:\IQO8L~\?~-l.?M\8~1~\021119. L a m t r o d i f i d : W d YU 10 09:21:55 1Y91 Notbd: c:\XLB8LM\?m-l . ? m ~ t ~ \ O 2 2 4 9 9 . Lut rodifiod: Tu0 Uar 09 13:53:43 1999 Job cod.: PrZntod: . W o d Uar 10 09:43:56 1999 -2: WEa (inmu 1 022499001 2 022499002 3 022499003 4 021499004 5 022499005 6 022499006 7 022499007 8022499008 9 022499009 10 022499010 11 022499011 12 022499012 1 3I 022499013 1 4I 022499014 1 3i 022499015 . '1 6i 022499016 1'1 022499017 i nI 022499018 1 91 022499019 l aI 022499020 21, 022499021 22 022499022 21 022499023 24, 022499024 25, 022499025 26, 022499026 27 022499027 28 022499028 29 022499029 30 022499030 31 022499031 32 022499032 33 022499033 3 4 022499034 35 022499035 36 022499036 37 022499037 38 022499038 39 022499039 40 022499040 41 022499041 42 022499042 43 022499043 44 022499044 45 022499045 46 022499046 47 022499047 48' 022499048 49 022499049 50 022499050 51 022499051 52 022499052 57 022499057 58 022499058 59 022499059 60 022499060 61 022499061 62 Of2499062 63 022499063 b4 022499064 61 022499065 66 022499066 67 022499067 68 022499068 69 022499069 70 022499070 71 022499071 77 022499077 78 022499078 79 022499079 80 021499080 81 022499081 62 022499082 83 022499083 84 021499084 85 022499085 86 022499086 87 022499087 88 022499088 89 022499089. -lolist Taxt * )(.QII Blank ' MeOU Blank Tar UO. RBLO2229-HZO Blk-1, 2/22/99 Tax 120. RBLO2229-RBLivorBlk-1, 2/22/99 ToX 120. RBLO2229-5-1, 2/12/99 Tox 120. RBLO2229-lOppb-1, 2/22/99 Tox UO,RSLO2229-25ppb-1. 2/22/99 Tox UO. ML02229-50-1, 2/22/99 _ _ ~ . Tox 120. RBLO2229-1OOppb-1, 2/22/99 Tox 120. RBLO2229-2SOppb-1, 2/22/99 Tox 120. kiBLO2229-50Oppb-1, 2/22/99 Tox 120. ItBLO2229-75Onnb-~1. .2~12.2199 ~~ TOx UO;RBLO2229-100Oppb-1. 2/22/99 . I(.ow Blank Tox 120 810. 1 4 5 1 4 4 . 2/22/99 Tox 120 DUO. 1 4 6 1 4 4 , 2/22/9Y Tox 120 DP30. 147T-a. 2/22/99 Tox 120 DUO. 148?44; 2/22/9Y - - Tox 120 DUO. 1 4 9 1 4 4 . 2/22/99 Tox U O . RBLO2229-25O~~b-CCl2l~/22/99 neon . I d Tox 120 810. 1501-c1. 2/22/99 Tox 120 DM). 1511-04, 2/22/99 Tox 120 DP30. 1521-e4, 2I2219Y Tox 120 DM). 1531-04; 2/22/99 Tax 120 Dwo. 1 5 4 1 4 4 . 2/2219Y Tox l20, RBL02229-250ppb-CClZ. 2/22/99 W&U Blank Tox 120 DRO. 1 5 5 1 - a . 2/22/99 T o x l1: LO m.157T-a; 2/22/99 T o x l1: IO DP30. 1581-04, 2/22/99 T o x lt iLO DNO. 1 5 9 1 4 4 , 2/22/99 Tox 1U!O, RBL02229-250ppb-CCl3, 2/22/99 ne08 1Uank T o x l11!O DM). 1611-05, 2/22/99 T o x ltl!O DP30. 1621-05. 2/22/99 Tox 1L1!O DIOQ. 1631-05. 2/22/99 mx I !O DP30. 1641-05. 2/22/99 mx 1 IO Dye. 1 6 5 1 4 5 . 2/22/99 Toxl !O, RBL02229-250ppb-CC14, 2/22/99 l&U llmk mx 1 !O D)co. 1 6 6 1 4 5 , 2/22/99 T o x l !O Duo. 1671-05, 2/22/99 T o x lU!O Duo. 1 6 8 1 4 5 , 2/22/99 TOX IU!O 810. 1691-65. 2/22/99 Tox 1U!O D100. 170?4S 2/22/99 Tox1U10. ~L02229-250pp&CEl1,2/22199 noon aIlMk Toxu10 Duo. 1 7 - 4 1 2/22/99 Tox ll!O Duo. 1 7 3 1 5 5 2/22/99 Toxu10 UNO. 1 7 4 1 4 5 2/22/99 99 !O W O . 1 8 0 1 5 6 2/22/99 TOX 110 810. 1 8 1 1 4 6 2/22/99 Tox UO W O . 1 8 7 1 4 6 2/22/99 T o X 120, RBLO2229-250pp&ccJ7, 2/22/99 w.08 Blank Tox 120 W. 183T46 2?22/99 Tox U O DR). l84I-06 1122199 TOX 120 m.i8sr-a a/zz199 Tox UO.DUO. 1 8 6 1 4 6 212219Y Tox 110 810. 1 8 7 1 4 6 2/22/99 Tox UO, RBLO2229-25Oppb-CCl8. noon a i d Tox U O DM. 1891-06 2/22/99 2/22/99 Tox . U O Dwo. 19OWC 2/22/99 Tox 120 Dm. 1 9 - 5 6 2/22/99 . Tax 110. RBL02229-250ppb-Ccl. 2/12/99 noon mu* T o X UO,1101-Ol-25Oppb-W, 2/22/9Y Tox 120. 110?41-250ppb-ll$D, 2/22/99 TOX U O , RBLO2229-25Oppb-cc1, 212219Y llsoll a i d TOX 120. mLo2z2g-mo ~ K - J ,a i z z i 9 9 Tox 120, RBLO2229-RBLiwrBU-1, 2/12/99 Tax 110. mL02229-5ppb-20 2122199 Tox 120, RBLO2229-10-2, 2/22/99 Tox 120, RBLo2229-25mb-2. 2/22/99 Tox 120; RBL02229-50&b-2; 2/22/99 TOx 120. RBLO2229-lOOppb-2, 2/22/99 Tox 120. RBLo2229-25op*2, 2/12/99 TOX 120. RBLO2229-5OOppb-2. 2/22/99 Tox 120, ML02229-7500pb-2, 2/22/99 Tox 110, RBLO2229-1OOOppb-2, 2/22/99 mD- Analyt. AMlyt. hlyt. hlyto stmdard 6t-d stud.rd Standard S M Sthd St.ld.rd Standard stud.+d mlyt. myt. hlyt. h1W. AUOlyt. Amlyt. pc Alulyt. AIUlyt. AMlyt. hlYt. hlyt. ac AIUlyt. hlyt. AIUlyt. -1s. Analye. ac -1s. hlyt0 hAlyt0 -yt. -ayct. hlyt. hlyt. , AIUlyt. -1s. mlytm hlyt. pc hlyt. -yt. hlvt. AMl+t. hlyt. cc -yt. Amlyt. h01yt. hlyt. E22: ac m1yt.0 AMlyt. hlyt. hlyt. hlyt. hlyt. QC hlyt. &ulyt.. hlyt. hlyu QC ss: A M l y t . hlyt. hlyt. miyt. &ulyt. 8tMdud stuldud standard Standud 8tuldu.d stud.rd 8tudud stud.rd sturd.rd 8 t d Dno 5.99 12.00 29.90 59-90 l20.00 299.00 599.00 898.00 1191.00 299.00 299.00 299 .OO 299.00 299.00 299.00 299.00 299.00 299.00 5.99 12.00 29.90 59.90 110.00 299.00 599.00 198.00 1198.00 m 6.648 6.583 6.563 6.562 6.521 6.565 6.539 6.457 6.474 6.461 6.565 7.321 6.464 6.377 6.450 6.465 6.583 6.409 6.389 6.387 6.407 6.542 6.458 6.474 6.476 6.496 6.448 6.490 6.551 6.480 6.459 6.442 6.474 6.451 6.531 6.559 6.441 6.480 6.470 6.448 6.477 6.537 6.461 6.472 6.450 6.448 6,466 6.548 6.469 6.472 6.411 6.565 6.481 6.376 6.502 6.456 6.512 6.433 6.464 6.481 6.479 6.417 6.477 . 157 1 0.82 Mt 463 1 46.18 M 671 m 250 1 14.54 M 21 314 . 352 24.11 -19 59.47 bb -1 981 123.26 Mt 3 2548I 156.08 M 19 4491 ' 645.67 IM 8 7211 1059.54 lQ# 18 8654 1 1163.23 1pI 5 - 421 1 41.08 I# 68 1 0.00 bb 36 1 0.00 bb 138 1 - 0.00 bb 147 1 0.00 YII 1692 1 228.87 bb -23 173 1 3.11 lQ# 52 105. 1 1. . 0.00 0.00 ml PI . * 1132, 1 141.57 IM -51 1354 1 178.69 PI -40 224055 133265.77 1111 201712 1 2 9 9 4 6 . 1 1 ml 161554 124128.41 IM 141519 161586 1657 121003.21 PI 123984.62 m 1 223.64 a 156 1 0.58 IQl 101189 129868.45 I X 116264 120222.41 In4 llZlO2 1 16632.67 IM 1454 1 193.43 Icn 803 1 96.74 1111 1191 1 169.1) ml -43 109 1 0.00 PI 2497 1 348.43 ml ' 179 1 4.02 bb 2s 1 8.91 bb 222 1 10.37 bb 1435 1 190.59 PI U 1 7 1 173.13 IQI -42 1610 1 225.59 )01 1441 1. X92.01 IQI U S 6 1 164.01 1p 1196 1 l 5 5 . u 1p 1402 1 185.75 ml l548 1!.207.50 1p -31 2042 1376 1 280.82 lk 1 181.81 bb 1603 i 2 ~ 5 . 5 7I# -26 U.90 1 169.U la -43 1936 1004 U97. 1 265.13 IU 1 126.59 I# 1 170.13 la 61 12 113 485 887 2050 3781 5715 7311 1 0.00 ml 1 0.00 1pI 1 4.70 101 1 49.55 101 1 109.18 ml 1 281.95 ml 1 139.86 m 1 826.54 1# 1 1063.66 )oI -100 -100 -84 -17 -9 -6 -10 -8 -11 8ouo 020199: p w n t l t a t o d QIL 03/09/99 md grfnt.d 0 03'110199 * I.0. 2 - .-- BACK TO MAIN - -._- 8-1. L b t X C: U(LBIL=\TACPl .m0\8.r0l.D8\0224998 Lut r P d i C i d X 1.0 wu 10 09:21x5s 1999 lrtboax Cx\W)S8L~\1~-l.m0U(.tMI\O22499. Lut 4rLi.d: W YU 03 13153r43 1999 Job cod.: rriiIt.6: thd YU 10 0 9 x 4 3 ~ 5 61999 -3: 8m -1.1i.t N i 0 ~ 2 4 9 9 0 0 n~eon n1.n~ 2 0.21~ 499_ 002 ~ HmO.M .Bla~ nk ~ ~ 3 022499003 TOX 156, mLo2zzg-mo Bik-1, 4 022499004 TOX 120. XBW2229-R8Ll-Blk-l, wow99 2/22/99 5 022499005 Tox 120, MLO2229-5ppb-1. 2/22/99 6 022499006 TOX 120. RBLO2229-10ppb-1, 2122199 7 022499007 TOX 120. mLO2229-25gpb-1. 2/22/99 8022499008 Tox 120, MLO2229-50-1, 2/22/99 9 022499009 TOX 120, RSLO2229-1OOppb-1, 2/22/99 10 022499010 TOX 120. XBLO2229-25Owb-1. 2122199 11 021499011 TOX 1 3 0 , '11)L02229-50Owb-l; 2/22/99. U 021499012 Tox 120, RBL02229-750ppb-1, 2/22/99 . 13 021499013 Tox 120. RBLO2229-lO0Oppb-1, 2/22/99 14 02~499014 w o n n i ~ k l5 022499015 Tox U O Duo. 1 4 5 1 4 , 2/22/99 1 6 022499016. Tox 120 Duo. 1461-G4, 2122199 17 022499011 Tux 120 D*o. 1 4 7 1 4 , 2/22/99 1 8 022499018 Tox 120 DNO. 1 4 8 1 4 2/22/99 U 022499019 Tox 120 Duo. 1491-rSI 2122f99 20 0 2 ~ 4 9 9 ~ 2 0ox UO,mLozz29-zsoOpb-rru. a i z z i 9 9 21 oz2499ozi w o n si& 22 022499022 Tox 120 Duo. 1 5 0 1 4 . 2;22/99 23 022499023 Tox 120 0190. 1 5 1 1 4 I . 2/22/99 24 022499024 l 2 0 Duo. 1 5 2 1 4 4 , 2/22/99 25 022499025 Tax 120 Duo. 1 5 3 1 4 , 2/22/99 _ _ 26 022499026 Tox l 2 0 LM). 1 5 4 1 4 . 2122199 27 022499027 Tox 120. RDL02229-2SO~&-CC12. 2/22/99 28 022499018 neon B l i n k 29 022499029 Tox 120 Duo. 1 5 5 1 4 , 2/22/99 - _ . 30 022499030 Tux U O DUO. 1 5 7 1 4 1 . 2/22/99 ji 0 2 ~ 4 9 9 0 3 1 TDX uom. 1 5 8 ~ 4 ;z i z z i 9 9 32 022499032 Tox WO DUO. 1 5 9 1 4 4 , 2/22/99 33 022499033 Tar 120. RBL02229-25O~~b-CC13. 2/22/99 34 02~499034 w o n niank 35 012499035 Tox 120 LM). 1 6 1 1 4 5 . 2/12/99 36 022499036 Tox 120 DNO. 1621-GS, 2/22/99 37 022499037 Tox 110 DUO..163145, 2/22/99 38 022499038 Tox 120 DUO. 1 6 4 1 5 5 . 2/22/99 39 022499039 Tox 120 DIR). 1 6 5 1 5 5 , 2122199 40 022499040 Tox 120, R1)L02229-250ppb-CCl4, 2/22/99 41 0 ~ ~ 4 9 9 0 4 n1eon ~ i a n l r 42 022499042 Tox UO DNO. 1 6 6 1 4 . 2122199 43 022499043 Tox UO Duo. 1 6 7 1 4 5 . 2122199 4454, 022499044 022499041 46j 022499046 47 022499047 Tox 110 Duo. 1 6 8 1 6 5 . 2122199 Tox 120 DUO. 1 6 9 1 4 5 ; 2/22/99 Tox 120 DNO. 1701-G5 2/22/99 Tox 120, RBL02229-250ppb-CC150 2122199 4 I1 022499048 HWIf BlMk 491022499049 Tox 110 DUO. 1 7 7 y 5 5 2/22/99 50' 022499050 Tox UO Dwo. 1 7 3 1 4 5 2/22/99 51 022499051 Tox U O DM). 1741-65 2/22/99 52 022499052 Tox U O Duo. 1 7 5 1 4 5 2/22/99 53 022499053 Tox 110 Duo. 1 7 7 1 4 6 2/22/99 5 4I 01149905& 55i 012499055 TOX u o . u HWU Blank ~ o ~ 2 2 ~ ~ - z 1 o p p b - c ca~i ca . z. i ~ s 56i 022499056' 120 Dw. 178?-G6 2/22/99 5'1"022409057 Tox U O Duo. 1191-66 2122199 5dI 022499058 591 022499059 .60I 022499060 61 022499061 61I 022499062 63, 022499063 64I 022499064 Tox U O IDID. 1 8 0 I 4 6 2/22/99 Tox U O DIR). 1 8 1 1 4 6 2/22/99 Tox 120 Dw. 1 8 2 1 4 6 2/22/99 * Tox l20, UL02229-25oppb-cc17~ 2/22/99 neon Blank Tox 120 LPR). 183?46 2122199 Tox 120 Duo. 1841-cC 2/22/99 . 6Si 022499065 66j 021499066 67 022499067 6 II 022499068 691 022499069 Tox 120 DNO.'1851-G6 2/12/99 Tox U O DBO. 186?46 2/22/99 Tox l20 DM). 1 8 7 1 4 6 2122/9 Tox UO. mL02229-25Oppb-ccl8, 2/22/99 .701 022499070 71 022499011 72 Of2499072 73, 022499073 74I 022499074 7 s' 022499015 76, 022499076 77 022499077 781022499071 191 022499079 801 022409080 81 022499081 82 022499082 83 022499083 I 4 022499084 85 022499085 86 022499086 87 Of2499087 88 022499088 89 022499089 AM*. -1*. -2yr. -yt. MY-IYQc --**== M y t . Qc -1Y- -.ac Amlyt. -l.Yt= -*.-P*aC -=rprlyt. -1** --ww.. ac 5.99 U.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 7.290 .7.289 7.247 7.204 7.261 7.174 7.198 7.192 199.00 7.101 299.00 7 ~ 1 1 1 299.00 7.123 . 299.00 7.171 299.00 7.089 299.00 7.113 199.00 7.105 299.00 7.112 299.00 . 7.04s 7.206 7.119 7i102 5.99 11.00 29.90 59.90 UO.00 199.00 599.00 898.00 1198.00 7.145 7.158 7.189 7.U1 7.118 7.117 7.117 ~ r rmr .. 54 117 233 361 802 1576 2494 2933 1 4.31 bb 1 29.94 bb 1 7 7 . u tat 1 129.43 bb 1 309.35 101 I' 624.54 1(10 1 998.96 1Dp 1 1177.74 1(10 -64 0 29 8 3 . 4 11 -2 689 1 263.31 IM -1) 55: 1 210.01'I?l -30 603 1 221.00 1(w -24 674 1 257.lZ IM -14 645 -18 552 -31 * 608 l. 230.17 hb -23 595 1 224.69 bb -25 636 1 241.37 bb 733 1 280.86 tat 372 ' 1 133.84 P( 560 1 210.39 P( IO2 1 23.IO bb -20 174 1 53.38 W -11 332 1 117.60 101 -2 742 1 284.60 W -5 1344 1 530.03 lQot -12 2033 1 810.91 lDIx -10 2898 1 1163.57 bbx -1 LAC BACK TO MAIN I ?riDt.d! ~~ W a d N u 10 09:43:56 Colpound 4: -13 1999 ~~~ .l(.rr Wi.1i.t ~ r t 12 1 022499001 W.m 81- 2 022499002 -OX 81- 3 022499003 Tax 110. RBW2229-100 Blk-I, 2/22/99 4 022499004 Tox 110, RBW2229-RBLlwrBlk-1, 2122199 5022499005 Tox 110. RBW2229-5ppb-1. 2122199 5.99 I 6 022499006 7 022499007 8 022499008 9 022499009 10 022499010 Tox 110, MW2229-1Oppb-1, 2121199 Tox 120. RBW2229-25ppb-1, 2122199 Tox 110. RBW2229-50-b-1, 2122199 Tox 110. RBLOZZ29-100-1. 2122199 Tox 120; RBw2i29-250*1; 2/22/99 11 022499011 Tox 120.'m~2229-500ppb-1. 2122199 u11 012499012 Tox 110, RBLO2229-75Oppb-1, 212219~ 02~499013 TOX 120, ~ ~ ~ 0 ~ ~ 2 9 - i o o o p pabm-ii9.9 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 8.421 8.477 8.418 8.414 8.408 77 1 98.38 bb 226 1 356.08 hb 355 i ai8.71 bb 582 1 912.03 bb 695 1 1167.68 bb -18 . 19 ~~ -3 8 -3 . 1 4 022499014 15 022499015 16 022499016 17 ozz4990i7 x.Q)I Blank Tox 120 DYD. 1 4 5 1 4 4 , 2122199 Tox 110 W. 1461-04, 2122199 ox 120 DPOO. i m - w , a m i 9 9 18 022499018 Tox 110 DIOD. 1481-04, 2121199 _ - 19'022499019 Tox 110 DDI). 149144 2122199 20 022499020 Tox 110. NLO2229-25kb-cEll. 2122199 21 022499021 w.oB &uk id*. 299.00 8.409 165 1 2SO.06 bb -16 T 22 022499022 23 022499023 24 022499024 25 022499025 26 022499026 27 022499027 28 022499028 29 022499029 Tox 110 W. 1501-C4. 2122199 Tox 110 m.15U-Or; 2122199 Tox 110 .m.1521-G4* 2122199 Tox 120 m.lS31-04. 2122199 Tox 110 Dwo. 154?G4, 2/22/99 Tox 110, MLO2229-2SOppb-CCl2, 2122199 x.Q)I B l d Tox 110 DIOD. 155144. 2122199 kulyt. QC 299.00 8 3 2 5 17? 1 272.61 bb -9 30 022499030 Tox 110 DUO. 157?-64, 2122199 31 022499031 Tox 110 DUO. 158144, 2122199 32 022499032 Tox 110 Dwo. 1591-04; 2/22/99 33 022499033 Tox 120. X8LO2229-250ppb-CC13, 1122199 QC 34 0 2 z m 0 3 4 x . 0 ~ai- 199.00 8.330 8.493 176 264.11 W -12 63 73.51 bb 35 02249903S Tox 120 WO. l b U 4 S . PI22199 36 022499036 Tox 120 DUO. 362345, 2122199 . 37 022499037 Tox 120 DUO. 163145. 2122199 38 0 ~ ~ 4 9 9 0 3 8TOX 110 w . is4r-o~;zizzi99 39 022499039 Tox 120 Duo. 165?-GS. 2122199 40 022499040 TO% 120, 111LO2229~250opb-CCl42,122199 41 02~49904i mox ai& 03 42 022499042 Tox 190 DUO. 1661-0s. 2122199 43 022499043 Tox 110 D100. 167145. 2122199 --**. . 44 022499044 Tox 110 0190. 1681-05. 2/22/99 45 022499045 TUX 110 Dwo. 1691-05. 2122199 . . -*. 46 022499046 TOX UO m.110'10.42122199 47 022499047 Tox 110. RBLO2229-2SOppb-Cc15; 212JI99 1 48 022499048 49 022499049 50 022499050 n o si 022499051 52 022499052 y.011 B l a n k Tox GO DM). 17lF-CS 2122199 Tox 110 D110. 1731-05 2122199 ox m.i741-c~z m i 9 9 Tox 110 DNO. 1751-05 2/12/99 53 022499053 Tox UO Dwo. 177?-06 2122199 1 54 022499054 "ox -0. USL02229-2SOppb-CCl6,2/22/99 5s 022499055 MIX ai- -*e QC AMlyt. -0 Aualyte PC AMlsm AMls. PC AMlYt. 299.00 8.388 299.00 8.388 199.00 8.413 203 316.51 bb 6 182 1 a79.11 m -7 174 1 266.95 bb -ll 56 02249905C Tox 120 DUO. 178146 2112199 Amlit. 17 022499057 Tox 120 WO. 179146 2122199 14.Zye. 58 0224Y9058 19 ozz4990sg 60 022499060 -< 61 022499061 62 022499062 63 0224Y9063 6 1 022499064 65 022499065 'I -*. 66 022499066 67 022499067 68 0224Y9068 TUX 110 DUO. 1801-CI 2122199 ox 120 DND. iiir-cs zizzi99 Tox l20 Bx). 1821-06 2122199 * Tox l20, ~2229-250ppb-cu7,2122199 y.08 81- Tox l20 Bx). 183146'2122199 Tmr l20 DIO. 1 8 4 1 4 6 2122199 Tox 120 Bx): l85?-06 2122199 Tox 120 m.lOS?-OC 2122199 Tox 120 DIIO. 1871-06 2122199 Tox 1 1 0 , NL02229-25Oppb-CEl8. 212119~ AMlYt. -lyre rcuryu QC mYrruluc. -1AMlyt. AMm l PC 299.00 8.409 299.00 8.415 111 159.39 m -47 1 217 1 339.78 bb 14 69 022499069 81- AMlYt. 70 022499070 Tox 120 Bx). 189?-06 21221Y9 71 oz2499on TOX uo mo. i90r-06 z i z z m AMlS. AMlyt. '72 022499072 Tok 120 og). 1911-06 2/22/99 73 022499073 Tox 110. RBLO2229-25Oppb-CCl. 2122199 Aaa1Ytm PC 299.00 8.332 118 1 186.51 xa -38 74 022499074 MOB Bl75 022499075 ox PO. I I O ~ - G ~ - Z S O ~ ~ z-l~22S1.99 hlyt. WYtm 8.414 76 022499076 Tox 1 1 0 ; 1 1 0 1 4 1 - 2 5 0 ~ ~ b -2~12.2199 -lit. 8.335 . 77 022499077 To% 120. RBL02229-250ppb-K1. 2122199 -1s. 78 022499078 lmox B l Ilot AMW. 79 022499079 Tox uo, NLO2229-100 Blk-2, 2/21/99 -1s. 80 022499080 Tox uo, NL02229-RBLlveralk-t, 2122lY9 w*. I 1 022499061 Tox 1 1 0 , RBLO2229-Sppb-2, 2122199 sundud .. 82 022499082 Tox 120. RBLO2229-1Oppb-2, 212219Y st.ad.rd 83 022499083 Tox 120. MLO2229-25ppb-2, 2122199 -)I 8.391 5.99 12.00 29.90 164 1 248.13 bb 205 1 319.96 1w lS7 1 236.58 bb 84 022499084 Tox lao, mLO2229-SOppb-2, 2/22/99 85 021499085 'rox 120. RBL01229-100ppb-2, 2122199 8uod.rd sc.nd.rd 59.90 8.360 110.00 8.406 47 1 46.47 1BI -22 86 1 113.94 -5 86 022499086 TOx 120, 111LO2229-25Opob-2, 2122199 87 Of2499081 TOX 120. ML02229-500ppb-I, 2122199 sund.rd StMdUd 299.00 599.00 8.423 8.335 189 1 292.04 bb -2 373 1 ' 610.46 bb 2 88 022499088 To% 120, mL02229-750Dpb-2, 2 m i 9 9 E9 022499089 Tox 120, RBL02229-1000ppb-2, 2122199 Stusdud st.nd.rd 891.00 1198.00 8.333 8.334 507 1 841.82 lS4 -6 720 1 1110.29 101 1 BACK TO MAIN : ?riBt.dI h d yu 10 09t43:56 1999 ca3poord S I a613 I I 1 1 1. I 1 i i 1 -J .'I I I 1 1 I i 3 012499003 Tax U O , BSL01219-1110 Blk-1, 2/12/99 4 022499004 To% 120. mLO1219-RBU-Blk-1, 2/12/99 5 022499005 Tox 120. RBL02219-5ppb-18 212219Y 6 021499006 ?OX 170. mLOl27.9-10-1. 2/22/99 7 022499007 Tax 120. RBLO2229-25ppt-1, 2/22/99 8 021499008 ?ox 120. mLO1229-50-b-1, 2122199 9 012499009 Tox 120. lll)LO2229-lOOppb-1. 2/22/99 10 022499010 11 022499011 12 0214Y90lZ 1~ 3 .022499013 14 012499Qlr 15 022499015 16 022499016 17 022499017 18 022499018 19 022499019 Tox 120. mLO2219-250-1. 2/12/99 Tox 120; LBL01219-500&-1~ 2/22/99 _ - ?ox 120. RBLO1229-75Oppb-1. 2/22/99 * ?ox 120. mLOl22Y.-~ 1OOOwb-t.. 2/21/9 w n ai& Tax 120 Dyo. 1451-01, 2/22/99 Tax 110 DR). 146.44. 2/22/99 Tox U O DR). 1471-04; 2/22/99 Tox I20 m.148F44, 2/22/99 'Tox 120 DM. 149?-0(, 2/22/99 St.ndUd Suadrrd Suldud -*e -lYt* -Yt. Ip.Lyt. -1- PO oznr99oio Tox 110, W O 2 2 2 9 - Z S O H l l , 2/22/99 21 022499021 xaox Blulk 9C AMlYt.' 22 022499022 Tox U O DM). 1 5 0 1 4 4 , 2/22/99 a3 o m 9 9 0 n Tox 110 DM). 15V-04. 2/22/99 24 022499024 Tox 120 Dpo. 1 5 2 1 4 4 , 2/22/99 -*a Anrlyt. -Yt* 25 022499025 Tox 110 PIO. 153?44, 2/12/99 26 022499016 Tox U O o*o. 1 5 4 1 4 4 , 2/22/99 a7 0 ~ 2 4 9 9 o n Tox U O , aaLO2229-25Oopb-ccl2, 2/22/99 28 022499028 neon B l a n k 29 022499029 Tox u o mo. 1551-01. 2/22/99 30 021499030 Tox 110 PIO. 1571-We 2/22/99 31 021499031 TOX u o rem. 1s8r-m, 2/22/99 32 021499032 Tox 110 moo.159144. 2/22/99 33 022499033 Tox 120. ~L02129-250pob-ccI3,2/21/99 34 022499034 w.og Blank 35 021499035 Tox 110 PIO. 16V-05, 2/22/99 36 022499036 Tox U O DNO. 162?-05, 2/12/99 37 022499017 38 022499038 39 022499039 40 021499040 41 022499041 42 022499042 Tox 1 2 0 DUO. 163?-G5, 2/22/99 Tox l20 Dxo. 164?-05. 2/22/99 Tox 110 Dxo. 1651-05. 2/22/99 Tax 110, RSL02129-250ppb-CC14, neon Blank TOX U O Dxo. 166145. 2/12/99 2/22/99 43 022499043 Tox 120 m.1 6 7 1 4 , 2/12/99 44 022499044 Tox U O DM. 1 6 8 1 4 5 . 2/22/99 AI=*. -Yt* PC Alulyt. --*Yt* -Yt. -*e CC AlUlYtO AMlYt. Alulyt. --**e * -*.QC mYt. AlUlyt. -*.m y t . 45 022499041 Tox 120 m.169?4S, 2/22/99 46 022499046 Tox 120 DM. 1 7 0 1 4 5 2/22/99 -*e AlUlYt. 47 022499047 Tox 110, RBL02229-25O~~b-CCl5,2/22/99 48 022499048 -OB ai& 49 022499049 Tox 120 DM. 171T-05 2122199 CC AZUlYt. AaUlYt. 50 022499050 Tox 120 Dwo. 173?45 2/22/99 AlUlYt. 51 021499051 Tax U O DNO. 174C-05 2/22/99 52 022499052 Tax 120 m.175?-05 2/22/99 AMlyt. AlUlYt. 53 012499013 Tox 120 W . 177C-Cb 2/22/99 54 oaz499054 TOX 120. m r . 0 2 a z 9 - z s ~ i 6 ,w 2 a i o s 55 022499055 neon Blank Aac-lYtm -le. 56 022499036 'Tax 120 DM). 178?-06 2/22/99 ' 57 022499057 Tox 120 OD10. 1 7 9 1 4 6 2/22/99 . 58 012499058 To% 120 DM. 1 8 0 l 4 6 112219Y 59 022499059 Tox 1 1 0 ma. 1 8 1 1 4 6 2/22/99 60 022499060 Tox 120 DM. l81.-06 2/22/99 -Yt. W haYwt .. AlUlyr. 61 012499061 Tox UO. ML02229-250opb-CCl7, 2/22/99 61 022499063 El& 63 0224Y9063 Tox 110 m.1 8 3 1 5 6 2121199 64 02249i064 fox l 2 0 m.l841-b( 2/22/99 65 021499065 Tox lZ0 DM). k 8 5 1 4 6 2/22/99 66 022499066 67 022499067 68 021499068 69 022499069 70 022499070 . 7 1 022499071 72 021499071 73 022499073 74 021499074 15 012499075 Tox 120 Dg). l o w - 0 6 2/22/99 _ _ ToX 120 Dg). 1 8 7 1 4 2/22/99 Tax 120. uIW1229-25ODDb-CCl8a 2122199 waid Tox 130 m.1 8 9 1 4 6 2/12/99 Tox 120 Dw. 19OI-06 2/22/99 Tax b o DM). 19Y-06 2/22/99 Tox 120.. R B W 2 2 1 9 - 2 S O ~ . 2/21/99 mon ai& Tox 120, 110?41-250pob-~, 2/21/99 76 022499076 Tox 120, 110?41-150ppb-nsD, 2/22/99 77 025499077 Tox 3.20, RBLO2219-2500pb-tXZ. 2/22/99 78 022499078 N.OH B l d 79 021499079 Tax 126, mLo2219-1110 But-2. 2/22/99 80 022499080 fox 120. mw1229-mLiWrBlk-1, 1/22/99 81 012499081 Tox 110; NL01219-50pbl. 2/21/99 81 021499082 Tox 120. ULO2219-lOppb-2. 1/11/99 standud 83 021499083 Tox l l O # ~W2229-15ppb-2. 2/11/99 84 ozz199081 Tox 120, m ~ o z i z ~ - ~ o o p b -wz z. w w Otdrd Itandud 85 022499085 Tox 110. )L11L02219-100ppb-2, 2/12/99 86 011499086 Tox 120, RBLO2229-15Oppb-1. 2/12/99 Lt.nd.rd . St-d 87 011499087 To% 110. ML02129-500p0b-2, 2/11/99 Sturd.rd 88 021499088 Tox 120. ML02119-75Oppb-1. 2/22/99 Stud.rd 89 012499089 TOX 110, ML01229-100Oppb-1, 2121199 Standard 5.99 11.00 29.90 59.90 lZO.00 199.00, 599.00 898.00 1198.00 299.00 299.00 299.00 299 .OO 299.00 199.00 299.00 299.00 299.00 S..-..9 11.00 29.90 59.90 . 120.00 199.00 599.00 8911.00 1198.00 9.373 9.374 9.398 9.380 9.345 9.347 9.289 9.280 9.280 9.274 9..279 9.262 . 9.262 9.287 9.277 9.287 9.289 9.367 9.295 9.253 9.297 9.311 9.278 9.295 9.294 9.293 9.294 " 8-D 020199: puamtitatd on 03/0/9 aad p r i n t d OD 03/10199 Du 44 1 8.41 BM -3@ 89 1 32.10 bb 7 148 1 63.32 101 6 281 1 133.38 bb 11 \. 610 1166 1 1 . 307.64 602.07 bb bm . 3 1 1788 1 930.80 bm 4 2 U 7 1 1110.50 bm -7 -20 . 480 1 239.14 bb 459 a 1 227.79 bb . 561 1 182.01 hb -6 571 1 287.06 hb -4 634 , 1 320.22 bb 7 sa4 1 293.99 bb -2 578 1 , 290.74 hb 489 1 243.59 bb 507 1 243.U bb 446 1 220.79 #I 466 1 231.31 1oI -3 1 62 1 17.85 1oI -40 142 1 '59.86 101 0 260 1 lZ1.65 101 2 566 1 184.38 101 -5 1158 1 591.74 bbx 0 1610 1 836.87 )oIx -7 1343 1 lZ24.94 W 2 . ., BACK TO MAIN CuuLtifY-c .uayry I1.oort rar-120 RAt L i v u I I i ILuapslt.dLif.tti d :cNo:d\W Y UU81L0~0\9r1~21-rl55. )1U99)9\ 8 ~ 1 ~ \ 0 2 2 4 9 9 A mthod: CI~8LM\?FT-l.M\X.thm\022499. Wt d f i d r nu YU O I 13853r43 1199 Job cod.: kintdt ' r(d rU 10 09r43i58 1999 1I 6: II !iw ddfl 'I #mu 1022499001 2 022499002 3 022499003 4 022499004 I.rpl.1i.t Taxt XeOH Blank y.011 a i d Tox 120. RBLO2229-~0Blk-1, 2/22/99 Tax 1 3 0 , RBLO2229-RdLlwtBlk-1. 2/22/99 as 7.314 7.310 ma.. C0na.m- , 348 1 248.61 101 -50 278 1 198.55 bb -60 5 022499005 Tox 120, RBLO2229-5ppb-1, 2/22/99 6 022499006 Tox l20, RBLO7.229-1Oppb-1, 2/22/99 - S Sundud 494.00 494.00 7.252 7.236 665 1 474.76 101 -4 847 1 604.74 101 22 I 7 022499007 8 022499008 9 022499009 10 022499010 ii 022499011 lz 022499012 TaX U O , -2229-25gpb-1, 2/22/99 Tox l20, RBLO2229-50-1. 2/22/99 Tax 120, RBLO2229-1OOppb-1, 2/22/99 Tox 120, RBL42229-250ppb-1, 2/21/99 TOX I Z O . . R B L O ~ ~ ~ ~ - S O Oi~ /~ z~ m- ~g, Tox l20, RBLO2229-75Oppb-1, 2/22/99 St.nd.rd StStandard St.ndud - 8 Sed 494.00 494.00 494.00 494.00 494.00 494.00 7.235 7.194 7.237 7.209 7.l22 7.145 768 1 148.31 bb 11 7l3 1 109.24 bb 3 583 1 416.56 hb -16 663 1 473.22 bb -4 675 1 482.12 M -2 673 1 480.59 bb -3 3 . 13 022499013 14 022499014 1s 0224990U 16 022499016 17 022499011 18 022499018 Tox 120, RBL02229-1000pOb-1, 2/22/99 y.08 Blank TOX 120 nm. i45r-c4. I I ~ ~ I J I Tox l20 W . 1 4 6 1 4 , 2122199 ' Tox 110 DK). 1 4 7 1 4 , 2/22/99 Tox 120 DK). 148144. 2/22/99 Stradrrd 494.00 &ulyt* A=- . -yta rarlrt. AnAlyt. 7.138 7.237 7.136 7.050 7.049 746 1 532.80 bb 389 1 278.17 )01 632 1 451.67 bb 421 1 300.56 bb 472 1 336.95 bb 8 -44 -9 -39 -32 . 1. 1I ' 19 022499019 Tox l20 OR). 149?-G4- 2122199 AMlYt8 20 022499020 Tox 120. RBLO2?29-25OppbCCll, 1/22/99 QC - 21 022499021 22 022499022 23 022499023 24 022499024 25 022499025 26 022499026 W X Blank Tox U O m.150144, 2/22/99 Tox l20 DSO. 151144, 2/22/99 Tox l20 DK). 152?4. 2/22/99 Tox 120 DK). 1 5 3 ? 4 , 2/22/99 ox 120 am. i54r-c4, 2/22/99 - AnAlyt. AnAlyt. A-Mwly.t. -1YU AMlyt. 27 022499027 Tox 120. RBLO2229-25O_ ~Db. -CCll. 2/22/99 PC 28 022499021 W E Blank 29 022499029 Tox 110 DIOD. 155?4, 2/22/99 -yt. Analyte 494.00 494.00 7.116 7.138 7.163 7.082 7.062 7.U2 7.031 7.060 7.212 617 1 441.04 bb -11 672 1 480.18 bb -3 329 1 235.U bb -52 481 1 343.87 bb -30 199 1 428.10 bb .-13 580 1 414.05 PI -16 402 1- 287.31 bb -42 555 1 396.73 bb -20 316 1 225.86 PI -54 I - - 30 022499030 TUX 120 CK). 15714. 2 m i g 9 31 022499031 TOX u o m.is8r-a; 21~2199 32 022499032 Tox l20 DK). 159?-U. 2/22/99 AIUlYt. Anal*. , AMlyte 33 022499033 Tox 120. lsL02229-25ODb-CCl3. 2/22/99 Qc 34 022499034 BWOH Blank ' JiMlyt. 35 022499035 Tox 110 Dyo. 161?-C5, 2/22/99 hlyt. 494.00 7.142 7.130 7.054 7.072 7.209 473 1 337.66 PI -32 464 1 331.42 -33 362 1 258.72 bb -48 654 1 466.87 bb -S 273.25 kM -45 36 022499036 To% 120 DUO. 162?35, 2/22/99 37 022499037 ox 120 DUO. is3r45, z/za199 rrulytm Arulyte 7.131 7.144 482.50 bb -2 420.31 bb -15 38 022499038 39 022499039 i 40 022499040 4 1 022499041 1 42 022499042 43 022499043 Tox 120 DUO. 164?-CS, 2/22/99 Tox 120 CK). 1_ CS.I-_ C_S_.._ 2122199 Tox 120. RBLO2229-25Oppb-CC14, 2/22/99 w.QB Blank Tox 120 CK). 166135. 2/22/99 ox SO OR).1671-05. Z I Z Z I ~ ~ AMlyt. AnAlYt. QC 494.00 7.063 7.160 7.119 7.223 7.068 431.38 bb -13 367.61 bb -26 558 1 398.50 bb -19 349 1 249.01 bb -50 497 1 355.24 bb -28 44 022499044 Tox l20 IXO. 168?b5. 2122199 7.130 586 1 418.91 kM -15 45 022499045 46 022499046 47 022499047 I 48 022499048 , 49 022499049 50 022499050 51 022499051 52 022499052 53 022499053 54 022499054 1- - S5 022499055 TOX IZO DM). i77r46 aizz/99 Tox 110. RBto2229-250~b-fx16. 2/22/99 lllnL '494.00 494.00 7.106 7.051 7.124 7.231 7.1l2 7.151 7.138 7.035 7.145 441 1 311.98 bb 353 1 252.19 542 1 387.24 PI 340 1 242.98 m 534 1. 381.17 bb I66.66 PI 352.10 IBI 161.71 PI 361.80 bb -36 -4s -22 -51 -23 -26 ..$. < * -29 -67 -2 6 56 022499056. Tox 110 PUO. 178146 212219Y 7.2ll 236 1 168.62 hb -66 57 022494057 To% 110 Dw. 179?46 2/22/99 58 022499058 Tox 110 LI)o. 180146 2/22/Y9 AMlyt. malytm 7.134 . 216 1 lS4.S8 bb -69 7.059 226 1 161.28 am -67 59 022499059 60 022499060 61 02349Y061 62 022499062 63 022499063 64 02249)064 61 022499065 66 022499066 67 022499067 68 022499068 Tox 120 Dwo. 1821146 21221YY T a x 120 Dw. 1821b6 2/22/99 . Tox 120, RBLO2229-2SDppb-CC17, 2/92/99 lh0H Blank f o x 110 Dyo. 163146 Z/ZZ/Y TOX IZO mm. i84r-06 ~ 1 ~ 2 1 9 )' TOX 120 D U O . - ~ ~ S ~21-2C21~9~ Tox l20 Dw. 186146 2/22/99 Tox 120 Dw. 187?-06 2/22/99 Tox 120, RBLO2229-25oppb-CCl8, 2/22/99 -*.-yt. AlUlYU QC AMlyt. &Alyt. mlyt. -Yt. AMlYt. PC 494.00 494.00 .7.037 7.035 7.141 7.131 7.142 7.137 7.135 7.143 7.145 231 204 571 . 1Y9 225 281 208 308 175 1 167.67 bb 1 146.02 bb ,t 407.90 bb 1 142.43 m 1 16P.54 bb 1 200.47 101 1 148.1 bb 1 220.14 bb 1 410.15 hb -66 -7 0 -17 , -71 -68 -59 -70 -55 -17 69 022499069 Y.#l .lank Arulyt. 70 022499070 71 022499071 a2 022499072 73 022499073 74 022499074 75 022499075 76 022499076 Tox l20 PUO. 189?46 2/22/99 Tox 120 CUO. 1901-GC 2/22/99 To* 120 W . 19211-06 2/22/99 ?ox 120. RBLO2229-250ppb-cE1, 2/22/99 M80H B h a k Tux 120. llO~-Gl-2IOpgb-BtS.2/22/99 Tox 120. iior-ci-zsoppb-mo, 2122199 Arulytm Myt. AMlyt. QC AnAlYt. Anrlyt. &ulyte 494.00 7.137 7.068 7.080 6.996 7.152 7.066 203.31 bb -59 .. 250.92 DM -49 zi8.67 m -56 389.67 bb -2 1 322 1 229.80 bb -13 366 1 261.34 bb -47 77 022499077 Tox lzo, RBL02229-250pOMQ. 2/22/99 Analyte 7.050 531 1 379.16 bb -23 78 022499078 79 022499079 80 021499080 81 022499081 81 021499082 83 022499083 84 022499084 85 022499085 86 022499086 87 022499087 88 022499088 89 022499089 XeOH Blank To% 130, RBLO2229-820 Elk-2, 2/22/99 Tox 120. RBLO2229-RdLfwnrBlk-1, 21221919 Tox no, RBLO2229-5pob-2. 2/22/99 Tox 120, RBL0222-10ppb-2, 21221Y9 Tox 120, RBLO2229-2Sp0b-2. 2/22/99 Tox 120, RBLO2229-50ppb-2, 2/22/99 Tox 120. RBL02229-100ppb-2, 2/21/99 Tox 120. mLo22~-25oppb-2,2/22/99 Tax 120, RBLO2229-5OOppb-2, 212219Y Tox 120. RBL02229-750ppb-2, 2122/99 Tox 120. RBLO2229-1OOOppb-2, 2/22/99 Myt. -8 Arulyt. Standud StuIdud Standard IltAudard standard StAndArd standard Standard Standard 494 .OO 494.00 494 .oo 494 .OO 494 .OO 494.00 494.00 494.00 494.00 7.208 7.197 7.169 7. iza 7.093 7.105 7.136 7.068 7.066 7.064 7.064 ' 308 1 219.86 101 -55 318 1 226.93 DM -54 . 670 1 478.83 101 -3 704 1 502.64 bb 2 756 1 540.16 bb I 748 1 533.96 bb 8 691 1 493.58 bb 0 732 1 523.07 IM 6 642 1 458.22 bb -7 638 1 455.91 bb -8 537 1 383.25 IM -22 i I aiibration: c:U C U I L ~ \ T ~ - ~ . ~ \ ~ \ O ~ ~ ~ ~ S . Lut d r i d : tmd yu i o o ~ t i a ~ ois)) mistdl *.d y.f 10 09144rOl 1999 1 + ompound 1 name: PFOS I orrelation coefficient r = 0.998526, r*2 = 0.997055 . alibration curve: 23.3472 x 297.446 ' esponse type: External Std, Area utve type: Linear, Origin: Exclude, Welghting: Null, Axis trans: None I. 2.95e4 1 3 1 1 I I I 1 Responsr f 3 1 .. . '. .I I 1 I I 1 BACK TO MAIN X 3 ."' . .. >ompound2 name: PFHS >>aohrrberlaattiioonnccuorevffei:ci6e.n7t3:0r7=50.9x8+9513571,.7P827= 0.979184 . lesponse type: External Std, Area ;urve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None 8.6%: 3 ....- . 1 : . .. .. 1' 1 I i 1 ?.esponss 1 .] I f 1 f X 1 rcnr0 OlOlS9: g l u n t i t a t d an 03109ISS .nd minted 011 031101SS bu Du I ... BACK TO MAIN X i I! :ompound 3 name: POAA I >orrelation coefficient r =0.995764,P2= 0.991546 , :allbration curve: 2.45337 x + 43.4900 - qesponse type: External Std, Area 3urve type: Linear, Origin: Exclude, Weighting: Null,' Axis trans: None 2.986 I 1 3 1' I i 1 ! I Respons 1 '1 1 1 1 I. i ! BACK TO MAIN 2ompound 4 name: M513 :orrelation coefficient r = 0.996106; e2 = 0.992227 , 2alibration curve: 0.577960 * x + 20.1 878 qesponse type: External Std, Area krve type: Linear,Origin: Exclude,Weighting: Null, Axis trans: None 720 .. .. BACK TO MAIN X Response .i . I . 0 r *-_A- roup OlOlSSr porrrtitated 011 0310#/S9 &udgrintd on 03110199 M ms 1 - .. 1 >ompound5 name:M613 1 ;orrelation coefficient: r = 0.996734,%2 = 0.993479 . >alibrationcurve: 1.88984 x + 28.4502' bsponse type: External Std, Area :urve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None I 2.34e2 I 3 1 i 1 i I Response 4 1 .*\ 3 i 1 I I 1 i .f 1. ; BACK TO MAIN I ..-_. . wQu-ainatiofy Caarel l burvr t iuo n R e p o r t c.libr.tiorrt c:\wIL~w~--I.M\c\o~~~ss~ Wt -stid: n a yu i o O M ~ : O S i s s s rrintdr Ikd YU 10 OSr44:Ol 1SSS :ompound 6 name: IS lesponse Factor: 1.40007 lesponse type: External Std, Area : w e type: RF 84; -- .. BACK TO MAIN -. .w I *! X Responsc C / . .. a.\ . ..... ..' .. e. BACK TO MAIN 1 *3 "3 P o l ~ a Tlyp.: Point of orioinx ? i t Y.iohtinu: . AniJ TruufO~tiOnI '1 1 I I 1 1 3 Rolativm hdphtr Abaolutr haislati norativa are.:. a01ut. uu: 3 1 8.036. pI.bld 0.000, D i s a b l d 1.030 kuut 185 .I 2 30.00 70.00 4.00 20.00 DiWd 10.00 , '50.00, 0.00. Pvbld Di8.bL.d , 3.00, Embld .O.OO, D W d .. .. .. .. . .. . 1 Iaterrul mt. x Quantify h n c m r conc. of Sturd.rd.X 1 User n? Valuer Wmr p..* hetom Znt.gr.tm Y i a d a ?UtorX Rmt.ntion Tip. b i n d I ndativm nmtantion Tim: TLU Y i a d a (&)I P.u 8.l.etionX 1.000000, D b . b l d 1.000000 2.000 6.563, mubled 0.000, Dimb1.d 1.117 I..mt " . bit-1 .nu . .- !- , 10.00. -ed 0.00. Disabled , , 3.00, Eublul 0.00, D i M b l d 3 'I BACK TO MAIN - . . . * . . . . a .t .. . I . .t . -. BACK TO MAIN J o i n W l y Lkms?told:' Il.duc. malt tailfrrg: rids. buelilu pudpn: 30.00 70.00 4.00 lbL.kti%mh d g h t : nholute highti r a m i n -I. Abolutm m a : , . ' 50.00, -lad 0.00, U i S m b l d 3.00. mUb1.d 0.00, piubld . . .. .&\ .. - 1 1 1 I 1 3 9 1 .I I 1' I mthod film tut mdifiad: Conpound: c: \WASSLYNX\?~-l.PM\~~~\O22493..rdb. Iri Ieb 1 6 10:30:39 1999 4: W 1 3 Printed: TU. mr 09 13:22:011999 c e )S.nu: x a t d 1uf.: Quantify R u m : c0nC. of standudax Vaer n? V . 1 ~ 1 Uamr ?e& T u t o r : Intmgratm W i n d o w Putor: -13 1-1 TIC Conc. A 1.000000, DiUb1.d 1.000000 a. ooo SOUP 020lQQ Ratention Tima (rku): mlativ. netontion T h : Tiuwinaa(riru): . - p..L li.1.ctiOa: 8.648, Eubld 0.000. Diaablad 0.130 wmarmat . rmftar 30.00 70.00 4.00 10.00 0h.bZ.d 10.00 . , 10.00. m u b l d 0.00. Dia.bl.6 3.00, Snab1.d 0.00, Diaablad LAC, 03todW BACK TO MAIN 1 I 1 I 1 .! i 1 I I 1 I 1 3 *I 1 1 -I I I 1 Rmtentioa T h I&) I Rmlative Ret8ntion T h : Time W i n d o w (&IS) : ?esk Selmction: M611 11RC Cone. A 1.000000, Disabled. 1.000000 1.000 9.591, mabled 0.000, Disabled 0.730 lie.wt - units: Pesk-to-p.ak . P l i h d . I 185 . DiSM8d 1 1 30.00 70.00 4.00 .' Draw wrtic.1 tiue.hold: Detect shoulders: Shoulder threshold: 10.00 DiS8bl.d 10.00 R8latiw height: Ahsolutm height: TmlAtiw area:. Absolute area1 * 500.0.000. , Dmi rur bbll.rdd 3.00, Pvbld n.oo. ~md .I I ,I 1 .. J - . BACK TO MAIN ---- - 1 1 1 1 I 1 3 .I 1' 1 I i 1 1 1 1 - I nothod R e p o r t U 8 U V01U.X U 8 U PO& ?=tort Intograt. w i n a a r metor: Is 1n00.i TIC c-. a 1.000000, D i u b l d 1.000000 2.000 7.410. Publd 0.000, D i u b l d 0.918 161 Dis&ld 1 'I 30.00 70.00 4.00 10.00 DZub1.d 10.00 10.00. .rubld 0.00. Dkrbld 3.00, Eubl.4 0.00, D h b l d i 1 I I i --.A- I ! BACK TO. . MAIN i R " . , BACK TO MAIN 1 >- 1 MADELINE 041098: FACT Equipment Log 98053 Quattro II, #a433 1 DATE. 03/01/99 ANALYST: arb 1 - STUDY NLT-ER FACT Method TOX 120 M A m rat liver D E S e O N liver extr. dilutions . 1 Injectionvolume,lO UC Mobile Phase: Cycle time: 16 min. Electrode: %Spray (AY.0 mMNH4CH3C02 0 3 TN-A- or stndrdnumber (A) 9'9011-1-9 Prepared (datelby): . * (Aj 2/12/99 mee (B) TN-A-2384 (B)2/20/99 mb Expires: (A) 08/12/99 * (B)8/18/99 '7 Store atroomtemp? or N . . other: i .. Guard column: None P q number:na . . .. Lot number: na 1. Analytical column: keystone betasil I size:2 x 1OOmm 1 I).pe:c18 . Sphere size:5 um I Partnuinber: 105-701-2 Lot numb= p8fl3 Saialnumbcr118739n 1 Analytical parameters: I Drying g'as (L/hr): 300 ESI neb&g-gas (Yhr) 15 Sheath gas (L/hr) 0 5 *! . . I L C Flow rate: 300uL/min. Back pressure: 120bar 23 HPLC solvent gradient:long2ex :\ 1 Time (Inin.) %B , 1) 0.00 40 2) 8.5 . 90 1 3 1.1.0 4) 11.5 90 -100 J . 5) 12.5 9 13.00 . 100 40 1 I .---A- 1 - - .. -- 1 Tuning Method Report Method: c:\MA~sL~N~\~Ts.PR~\A~Q~DB\PF~S 1 Printed: Mon Mar 01 17:17:40 1999 MadJim WIOW I 1 3 1 1 I I Ms SOURCE ( ESP- 1 I Capillary Cone Extractor 1 RF Lens Source Block Temp. Desolvation Temp. 3 3 Pressures I Analyser Vacuum Gas Cell J 1 . 1. I I Set Rdbk 3.00 3.00 45 -4 6 . 5 -4 0.2 150 153 250 251 Rdbk 6.4e-5 5.3e-3 MS1 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier :\ Ms2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier BACK TO MAIN ' Page 1 1Acwl1oln9 . . S e t Rdbk 15.0 15.0 I 2.0 2 0.0" 5 5 650 . .. S e t Rdbk 15.0 15.0 20 * 20 2 .o 0.0 40 41 0 18 650 I t a n n i n g Mathod Report Mothod: c: W ~ R A T s . P l u J ~ B \ % i x l 2 0 A ust ~ o d ~ r i dw:on w 01 17:16:3m 199s Printd: llon Mar 01 17:17:51 1999 aolvmt D o h y I minm 1 I 0.00 mti~n I 1 m or 3 ma. at.( rm- 1 . .. 91 1 1nt.k Churn.1 Dmlay I Soca ) I 0.03 span ( Dalton. I I 0.00 Start T i m ( Una ) : 0.00 End T h o ( Urn I I 13.50 mpoata I 1 Churnel Parent Daughter -11 (Seer1 Cot1 m.rgy (mvt Con0 f V I 1 399.00 80.00 0.25 45 60 2 399.00 99.00 0.25 45 60 3 399.00 130.00 0.25 45 60 1 Nnation 1 2 WW of. 3 -0 Rim f ES?- I nepeata 1 Channel Parant 1 499.00 2 499.00 3 499.00 Daughtu 80.00 99.00 130.00' 1 Dm11 (Sacal C o l l mugy (OW Con. 0.25 45 0.25 0.25 . 45 4s . 60 60 60 IV? N M t h I & l a )cll(OfzIcuS-(r*) . 1 . 1nt.r Churnel h l a y I Saea 1 I Span t Dalton. I I S t a r t T i u I Nina 1 I . MTiuIWirrs): 0.03 0.00 0.00 13.50 R*p..tr : 1 w.1paient Daughtar -11 1s.u) COIL m e r w WI c ~ n eI v 1 . f 1 299.00 80.00 0.25 4s 35 i 2 299.00. 99.00 0.25 45 35 n-ti~n t 4 m oc a m.. a n rm- j- I n t o r Uunn.1 Delay I Sau'l I 0.03 Span ( Dalton. ) : 0.00 I S t a r t T i m (niru 1 I. . 0.00 end T i m I xina i I 13.50 . hpeata : 1 Churnel Parmnt Daughter. -11 (5.~81 Coll &orgy (.VI 1 413.00 119.00 . 0.25 2 413.00 169.00 0.25 1 .3 413.00 219.00 0.25 30 20 * 30 Con. ( V I 15 15 15 I n t o r Cham-1 I k l a y I So- ) I 0.03 Span I Dalton. ) 1 0.00 S t a r t Tima I UM 1 t 8 0.00 13.50 1 469.00 169.00 0:25 45 * 30 2 469.00 219.00 0.25 45 30 3 469.00 119.00 0.25. 4s 30 BACK TO MAIN Fag0 1 .:? P . . .i 1 569.00 169.00 0.25 4s 35 2 569.00 219.00 0.25 45 35 3 . 569.00 119.00 0.25 45 35 )uno+iOn I 7 10# of 1 ha. ?air I E* 1 Intrr C h u r l t h l i y ( Srcr 1 : 0.03 Span I Daltona ) : 0.00 S t a r t T h e I Nina I I 0.00' Lnd Tllu I N i M . ) I 13.50 Repeats I .& Ch.nn.1 Paront Daughtar -11. IS.ul Coll Enerqy (OW Con. 1 427.00 80.00 0.25 40 60 IV 1 1 - Masslynx Sample List I 1 Sample List C:\MASSLYNX\rats.PRO\SarnpleDB\030199c.SPL Printed: Mon Mar 01 17:17:14 1999 i I Flle Name Flle Text 1 1 030199001 MeOH Blank 2 030199002 MeOH Blank 3 030199003 TOX120, RBL0222SH20 Blk-l,2/22/99 4 030199004 Tox 120, RBL02229-RBLiverBlk-ls2/22/99 5 030199005 TOX120, RBLO2229-5ppb-l,2/22/99 6 7 1 '8 9 10 11 1 12 13 030199006 030199007 030199008 030199009 030199010 030199011 030199012 030199013 Tox 120, RBLO2229-lOppb-1,2/22/99 Tox 120, RBLO2229-25ppb-l,2/22/99 Tox 120, RBLO22295oppb-l,2/22/99 Tox 120. RBLO2229-lOO~~b-l.2/22/99' Tox 120; RBLOZZ?9-25Oppb-l; 2/22/99 TOX120, RBu)2229-5OOpp~l2, /22/99 Tox 120, RElLO2229-75Oppb-i,'2/22/99 fox 120, RBLO2229-1OOOppb-l,2/22/99 3 14 030199014. MeOH Blank 15 030199015 Tox 120 DNO. 125F-GZ 2/22/99 D5 16 030199016 Tox 120 DNO. 126F-G2,2122/99 D5 17 030199017 Tox 120 DNO. 127F-G2,2/22/99 D5 18 030199018 Tox 120 DNO. 129F-G3,2/22/99 D2 I 19 030199019 fox 120 DNO. 130F-G3,2/22/99 D2 20 030199020 TOX120, RBLO2229-25Oppb-CCl,2/22/99 21 030199021 MeOH Blank 22 030199022 TOX120DNO. 131F-G3,2/22/99 D2 23 030199023 TOX120DNO. 132F-G3,2/22/99 DZ 24 030199024 Tox 120 DNO. 133F-G3,2/22/99 D2 I 25 030199025 Tox 120 DNO. 134F-G3,2/22/99 D2 26 030199026 .TOX120 DNO. 135F-G3,2/22/99 D2 27 030199027 TOX120. RBLO2229-25Oppb-CC2, w 9 9 28 030199028 MeOH Blank 29 030199029 Tox 120 DNO. 136F-G3,2/22/99 02 I 30 030199030 TOX120 DNO. 137F-G3,2/22/99 D2 31 32 33 i .34 35 38 - 37 _1 38 39 030199031 030199032 030199033 030199034 030199035 030199036 030199037 030199038 030199039. Tox 120 DNO. 138F-G3,2/22/99 D2 Tox 120 DNO. 139F-G3,2/22/99 D2 Tox 120 DNO. 141F-G3,2/22/99 D2 Tox 120, RBLO2229-25Oppb-CC3,2/22/99 MeOH Blank Tox 120 DNO. 142F-G3,2/22/99 D2 Tox 120 DNO. 143F-G3,2/22/99 D2 Tox 120. RBLO2229-25Oppb-cC4.2/22/99 MeOH Blank 40 030199040 TOX120,106F-Gl-250ppb-MS. 2/22/99 41 030199041 TOX120,106F-G1-250ppb-MSD, 2/22/99 42 030199042 Tox 120, RBLO2229-2!5Oppb-CC5,2/22/99 1 43 030199043 MeOH Blank 44 030199044 TOX120, RBL02229-H2O Blk-2,2122199 4s 030199045' .TOX120, RBL02229-RBLhrerBlk-l,2/22/99 48 030199046 Tox 120, RBL02229sppb2,2/22/99 1 47 030199047 Tox 120, RBL02229-1Oppb-Z2/22/99 48 030199048 Tox 120, RBL02229-25ppb-2,2/22/99 49 030199049 Tox 120, RBLO2229-5Oppb-2,2/22/99 . 50 030199050 Tox 120, RBLO2229-1OOppb-2,2/22/99 1 51 030199051 Tox 120, RBLO2229-25Oppb-2.2/22/99 52 030199052 Tox 120, RBLO2229-5OOppb-2,2/22/99 53 030199053 Tox 120, RBLO2229-75Oppb-2,2/22/99 I 54 030199054 Tox 120, RBLO2229-1OOOppb2,2/22/99 1 1 I i BACK TO MAIN Page 1 MS Flle TOXl20A TOXl20A TOXl2OA TOXl20A TOXl2OA ' TOXl2OA TOXl2OA TOXl20A TOXl20A TOX120A TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOX12OA TOXl2OA TOX12OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA ' ' TOXl2OA TOXl2OA TOX120A TOXl2OA TOX12OA TOX12OA TOXl2OA TOXl2OA TOXl2OA TOXlZOA TOXl2OA TOXl2OA TOXl20A TOXl2OA .-< TOX12OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl2OA TOXl20A TOXl20A TOX12OA Inlet File Bottle long2 91 long2 91 long2 . l long2 2 long2 3 long2 4 long2 5 long2 6. long2 7 long2 8 long2 9' long2 long2 long2 long2 long2 10 . 11 91 12 13 long2 14 long2. * 15 long2 16 long2 17 long2. 92 long2 18 long2 19 long2 20 long2 21 long2 22 long2 23 long2 92 long2 24 long2 25 long2 26. long2 27 long2 28 long2 29 long2 long2 long2 long2 93$ 30' * 31 32 long2 93 long2 33 long2 34, long2 long2 long2 . long2 , long2 long2 long2 long2 long2 long2 long2 long2 long2 35 93 36 37 38 39 40 41 . 42 43 44 45 46 Factor I Sample 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Standan 0 Standan 0 Standarc 0 Standan 0 Standan 0 Standan 0 Standan 0 Standarc 0 Standan 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte . 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 Analyte 0 QC 0 Analyte 0 Analyte 0 Analyte 0 QC 0 Analyte 0 Analyte 0 Analyte 0 QC 0 Analyte 0 Analyte 0 Analyte 0 Standai 0 Standar 0 Standai 0 Standai 0 Standa 0 Standa 0 Standa 0 Standa 0 Standa Date: Analyst: I i I 3 1 7' ! i. I i i .i 11 hdyte3: 3 ! i .i .I t 1 Ir FACT: DATA REVIEW-SUMMARYFORM -- BACK TO MAIN c . y-.: . I 3-b LOQ for thisdataset: I ' I I . I 1 I .1 3 '1 ,l I i I .I I -1 1 I I 1 I t QwntitycQ=P-diwmlmuyE.por+ faXl2OODlO~tli~Ocorpu213 S u p h list: Y : w 1 . ~ ~ \ 0 3 O U S a last d i d : Yon Yrr 08 09:49:26 1999 mtbod: Y: b a t m . p a a b ~ \ 0 3 0 9 9 a Last r0dCii.d: Nem Yrr 08 10:03;52 1099 Job cod.: printd: YOa 1Qr 08 10:U:49 1099 eU1. 1 030199001 2 030199002 3 030199003 4 030199004 5 030199005 6 030199006 7 030199007 8 030199008 9 030199009 10 030199010 11 030199011 12 030199012 13 030199013 14 030199014 15 030199015 16 030199016 17 030199017 18 030199018 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 24 030199024 25 030199025 26 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030l99033 34 030199034 35 030199035 36 030199036 37 030199037 39 030199038 39 030199039 40 030199040 41 030199041 42 030199042 13 030199043 44 030199014 45 030199045 46 030199046 47 030199047 4s 0~0199om 49 030199049 SO 030199056 51 030199051 52 030199052 53 030199053 54 030199054 lkrpl aa i s t 2 u t ' ' WOH Bilank HOOH Bilank Tox 12.O, RBLO2229-lt20 Elk-1, 2/22/99 Tox 120, 118102229-RBlivorBlk-1, 2/22/99 Tox 12:O, RBL02229-Sppb-1, 2/22/99 Tox 12lo, RBLO2229-10-1, 2/22/99 Tox 12IO, RBLO2229-25ppb-1, 2/22/99 Tox 12'0, RBLO2229-5Oppb-1, 2/22/99 Tox 12io, RBLO2229-loo&-1, 2/22l99 Tox 1210, 1IBLo2229-25Oppb-1, 2/22/99 Tax 13IO, RBLO2229-5OOppb-1, 2/22/99 Tox 1210, RBLO2229-750ppb1, 2/22/99 Tox 1210. RBLO2229-lOOOppb-1, 2/22/99 noan EIld Tox 1210 Dwo. 125102, 2/22/99 Ds Tox 12:O DUO. 126102, 2/22/99 U5 Tox 12IO DUO. 127.42. 2/22/99 s Tox 12:O DUO. 12914, 2/22/99 D2 Tox t 210 DUO. 130143, 2/22/99 D2 Tox 12:0, RBLO2229-250ppb-ccl, 2/22/99 w.ox Bllulk Tox I210 DUO. 13LI-03, 2/22/99 D2 Tox 12i0 DMO. 13-43, 2/22/99 D2 fox 12:O 0110. 133F43, .2/22/99 D2 Tox 12:O DUO. l34?43, 2/22/99 D2 . T o n 1310 DUO. 135I43, 2/22/99 D2 Tox 12IO. RBLO2229-25O-~-pk-CC2,2/22/99 w.QH Bl&k Tox 120 0110.. l36F-03, 2/22/99 D2 'fox 120 DNO. 137Z-G3, 2/22/99 D2 fox 120 DUO. 138F43, 2/22/99 D2 fox 120 0110. 139143, 2/22/99 D2 Tox 120 Dwo. 14lFG3, 2/22/99 D2 Tox 120, RBLO2229-250ppb-co. 2/22/99 UaOH Blank Pox 120 DNO. 142F-03, 2/22/99 02 . Tox'l20 DUO. 143F-03, 2/22/99 D2 Tax 120, RBLO2229-25Oppa-cCr, 2/22/99 UaOH Blank Tope 120, 106F-Gl-250ppb-XS, 2/22/99 TOr 120, 106F-Gl-250p&-MSD, 2/22/99 Tox l 2 0 , RBLO2229-250pp&cQ, 2/22/99 w.QH Blank Tor 120, RBLO2229-AZO Blk-2, 2122/99 T0x 120. RBLO2229-RBLivuBlk-1, 2/22/99 Tar 120. RBLO2229-5ppb-z, 2/22/99 Tool 120. RBLO2229-lOppb-2, 2/22/99 ToX l20. RBLOZ229-ZSppb-2, 2/22/99 . lox 120, RBLO7.229-SOppb-2, 2/22/99 Tox 120, RBLO2229-100-2, 2/22/99 TOX 120, RBLO2229-25Oppb-2, 2/22/99 Tor 120, RBLO2229-500ppb-2, 2/22/99 TOX 120, RBLO2229-7SOgpb-2, 2/22/99 T- 120, -2229-lOOOppb-2, 2/22/99 BACK TO MAIN -- si 6rp m Ari. nil.zrot. thonag8 I0.r 7.929 W09 1 286.71 MI 7.913 1871 1 4.03 Ht 7.959 1689 1 0.00 Ht 7.964 2245 1 ' 13.96Ht s.99 8.073 3267 1 . 41.14 bbX 587 12.00 7.899 2555 1 22.20 bb 85 29.90 59.90 120.00 7.927 8.007 8.023 2937 1 4107 1 6367 1. . 32.36 bb 8 63.44 m 6 123.50 Ht 3 299.00 8.002 12988 1 299.41 29l 0 599.00 8.043 1544 1 633.05 bb 6 898.00 8.017 37384 1 947.68 bb 6 1198.00 4.000 45478 1 1162.75 hb ' -3 7.871 l341 1 1 0.00 bb 7.858 1796 1. 2.05 bb 7.875 1820 1 2.69 bb 7.857 1645 1 0.00 bb 7.895 2289 1 15.14 bb 7.879 2367 1 17.21 Ht 299.00 8.051 W24 1 .260.53 bb -13 7.896 1450 1 0.00 bb 7.819 2498 1 20.70 M4 7.827 2470 1 19.95 Hf 7.901' 2356 1 16.92 H4 7.822 2315 1 15.83 H4 299.00 7.833 8.005 2363 1 10471 1 17.12 IOf 232.56 XU . -22 7.919 ' 1443 1 0.00 bb 7.887 2196 1 12.67 H4 7.869 1888 1 4.49 IQI 7.854 1834 1 3.07 IQI 8.020 2358 1 16.99 bb 7.869 26l2 1 23.74 bb 299.00 8.017 11401 1 257.26 bb -14 7.933 l302 1 0.00 bb 7.854 2228 1 13.53 H4 7.929 2526 1 21.41 bb 299.00 8.023 11556 1 261.39 bb -13 7.932 1265 1 0.00 IDI 8.031 24699 1 610.36 M4 299.00 1.015 8.043 7.864 25982 1 ll767 1 1409 1 . 644.70 H4 266.98 bb -11 0.00 m 7.885 7.898 5.99 7.915 1689 1 1341 1 1884 1 . 0.00 0:oo m 4.39 bb -27 12.00. 7.815 1952 1 6.18 M4 -19 29.90 7.995 2830 1 29.51 lQl -1 59.90 8.011 3802 1 55.34 bb --I 120.00 299.00 7.988 8.002 6278 1 12779 I .121.15 bb 1 293.88 bb -2 599.00 7.997 23132 1 568.95 M4 -5 898.00 1198.00 8.045 8.031 32672 1 . p 11 822.46 bb -8 1251.30 bb 4 1 -1 1 i I 1 3 1 1' .I I I i 1 0 1 1 I I .1 .I i - bqorrd 2: Rrm an u 1030199001 U t Text x.oH aIlank 2 030199002 w( Bllank 3 030199003 Tox 1.ZIO, RBL02229-HNI Blbl, 2/22/99 4 030199004 Tax 1.210, llBLO2229-RBLivmrBlk-1, 2/22/99 5 030199005 Tox 1.210. -2229-Sppb-1, 2/22/99 6 030199006 Tox 1-110. RBLO2229-lo&-1, 2/22/99 . 7 030199007 T o x 1.110, RBLO2229-25ppb-1, 2/22/99 8 030199008 Tox 1.1!O, RBLO2229-5Oppb-1, 2/22/99 9 030199009 Tox 1.1(0, RBLO2229-100&-1, 2/22/.99 10 030199010 Tox 1.I!O, RBLO2229-250&-1, 2/22/99 11 030199011 Tox 1110, RBLO2229-5OOppb-1, 2/22/99 . 12 030199012 13 030199013 14 030199014 _ - Tor 1.210, BBLO2229-750ppb1, 2/22/99 f o x 1.1!O. RBLO2229-1000-1, IhOH Blhk . 2/22/99 15 030199015 Tox l20 DNO. 125141. 2/22/99 W 16 030199016 Tox 120 DNO. l261-Q. 2/22/99 D5 ' 17 030199017 ?ox 1.210 DNO. 127?+2; 2/22/99 D5 18 030199018 19 030199019 fox 1 !O DB10. 129103, 2/22/99 D2 Tox 3.110 DUO. 130103, 2/22/99 D2 20 030199020 Tox 1.1IO, RBLO2229-250ppb-ccl, 2/22/99 21 030199021 w( BIlank 22 030199022 Tox 1.210 Dppo. 131203. 2/22/99 D2 23 030199023 Tax 1.2:O DNO. 132.43. 2/22/99 D2 24 030199024 25 030199025 Tax 1.210 DNO. 133243, 2/22/99 D2 lox 1.z10 DRD. l34103, 2/22/99 D2 26 030199026 .Tax 1.210 DNO. l351-63, 2/22/99 D2 27 030199027 Tax 1.2IO, lUM2229-250ppb-ccz, 2/22/99 28 030199028 W X E,lank. 29 030199029 Tax 1.210 DNO.. 136203. 2/22/99 D2 ' 30 030199030 Tox 1.210 DNO. 137143, 2/22/99 D2 31 030199031 32 030199032 ToX 1 IO DNO. 1381-03, 2/22/99 D2 Tox 1..Zz!O DNO. 139P-G.3, 2/22/99 D2 33 030199033 34 030199034 Tox 1 10 DNO. 14tP43, 2/22/99 D2 Tax 1.a10, RBLOW9-250pPb-c0, 2/22/99 35 030199035 nm.08 Ellank 36 030199036 37 030199037 38 030199038 39 030199039 _ _ ToX 1.1!O DNO. 142t-03. 2/22/99 D2 .Tor '1.2!O DNO. 1432-63. 2/22/99 D2 Tox 1.z!O. BBLO2229-250wb-cc(, 2/22/99 x.oH Bl&k 40 030199040 Tan 120, 106P-Gl-250ppb-W, 2/22/99 41 030199041 T o r 120, 106P-Gl-25Oppt-WD, 2/22/99 42 030199042 Tox 120, RBLOZ229-250ppb-CC5, 2/22/99 43 030199043 lwpI Blank 44 030190044 ?Ox 120, RBL02229-R20 Elk-2, 2/22/99 . 45 030199045 46 030199046 47 030199047 T o x 120, RBLO2229-RBLiwrBlk-1, 2/22/99 Tox 120, RBLO2229-5ppb-2, 2/22/99 ToX 120. pBLO2229-1Oppb-2, 2/22/99 41) 030199048 TOX 120, RBLO2229-2Sppb-2, 2/22/99 49 030199049 T- 120, RBLO2229-50-2, 2/22/99 50 030199050' T- l20, RBLO2229-100pp&2, 2/22/99 51 030199051 Tor 120, RBLO2229-250&-2, 2/22/99 ' 52 030199052 T a 120, RBLO2229-500ppb-2, 2/22/99 53 030199053 Tox 120, RBLOW9-75Oppb-2, 2/22/99 54 030199054 Tor 120, RBLO2229-1000~2, 2/22/99 - _ .... BACK TO MAIN rr 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 6.587 6.607 6.614 6.550 6.558 6.567 6.552 6.583 6.560 6.553 209 1 525 1 . 470 1 820 1 .1349 1 2570 1 5924 1 12321 1 19614 1 24476 1 5.43 bb 20.86 1Qp[ 248 18.15 MI 51 35.19 191 18 61.01 )PI .2 120.49 MI 0 283.94 191 -5 595.64 MI -1 951.00 191 6 1187-.90 191 -1 299.00 6.589 5.920 299.00 6.538 6388 1 195 1 4911 1 .257.80 bb -14 4.74.m 234.55 MI -22 299.00 6.559 5229 1 250.07 M4 -16 299.00 6.570 5209 1 249.09 bb -17 299.00 6.575 6.556 6.501 4836 1 4389 1 5140 1 230.91 MI 209.12 bb 245.70 bb -18 . .4 LZ . 6.605 5.99 6.612 89 1' 84 1 0.00 bb -100 ' l2.00 29.90 ' 59.90 l20.00 299.00 . 599.00 898.00 ll98.00 6.539 6.532 6.541 6.526 6.537 6.531 6.590 6.370 407 1 702 1 1467 1 . 2500 1 6330 1 11998 1 17964 1 .<4n4 i 15.10 191 26 29.46 BPf -1 66.73 11 117.05 M4 -2 303.61) MI 2 579.90 MI -3 870.59 191 -3 1202.41 IS4 .o BACK TO MAIN bl#Da 3: +mum 1030199001 2 030199002 3 030199003 4 030199004 5 030199005 6 030199006 7 030199001 e 03oiss008 9 030199009 10 030199010 11 030199011 12 030199012 13 030199013 14 030199014 15 030199015 16 030199016 17 030199017 18 030199018 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 24 030199024 25 030199025 26 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 34 030199034 35 03b199035 36 030199036 37 030199037 38 030199038 39 030199039 40 030199040 41 030199041 42 030199042 43 030199043 44 030199044 45 030199045 46 030199046 47 030199047 48 030199048 49 030199019 50 030199050 51 030199051 52 030199012 53 030199053 54 030199054 Gldist Tur+ IhOH Blank I*oa Blank Tor 120, RBLO2229-XZO Blk-1, 2/22/99 Tor 120, RBL02229-RBLivarBlk-1, 2/22/99 Tor 120, RBLO2229-5pp&l, 2/22/99 T o r 120, RB102229-10ppb-1, 2/22/99 TOX 120, RBLO2229-25ppb-1, 2/22/99 T o r 1 2 0 , RBLO2229-SOPgrl. 2/22/99 T- K O , NLO2229-1OOppb-1, 2/22/.99 TOX KO, RBL02229-250ppb-18 2/22/99 Tor K O , RBL02229-SOOppb-1, 2/22/99 Toll 120, RBL02229-75Oppb-1, 2/22/99 .Tor 120. RBLO2229-1OOOppP-1, 2/22/99 m &OH B h k TO^ KO DUO. ~ I U 2,m / s s T o r K O DNO. 126P42, W W 9 9 05 Tor L20 Om. 12m-GZ, 2/22/99 05 ToX l20 0110. 129143. 2/22/99 02 Tor 120 ONO. 130143, 2/22/99 02 TOX 1208 RBLO2229-250ppb-ccL, 2/22/99 BbOX Blmk T o r 120 0110. 13l?43, 2/22/99 02 TDpr 120 0110. 132F43, 2/22/99 02 Tor 120 DNO. 133F43, 2/72/99 02 TOX 120 0110. 134143. 2/22/99 02 .Tor 120 0110. 135143, 2/22/99 02 120, RBL02229-250pPbcQ. 2/22/99 I*oa Blank TOn l20 ONO. ,136143, 2/22/99 DZ Tox 120 ONO. 137143, 2/22/99 02 TOX 120 0110. 138F43, 2/22/99 02 T o r 120 0110. 139143, 2/22/99 02 TOX 120 DNO. 1411-G3, 2/22/99 02 TOX 120, RBLO2229-250pPb-c0, 2/22/99 HeoH Blank TOX 120 0110. 142F43, 2/22/99 02 .TOX i20 DNO. 143F43, 2/22/99 02 ?Or 120, RBLOZ229-250ppb-fcr, 2/22/99 BhOX Blmk TOX 120, 1O6F-G1-250ppb-US8 2/22/99 TOX 120, lOW-Gl-25Oppb-US0, 2/22/99 TOX 120, RBLO2229-250ppb-m, 2/22/99 I*oa Blank TOX K O , RBLO2229-AtO Blk-2, 2/22/99 T o r 120, NLO2229-RBLiwrBlk-1, 2/22/99 T o r KO, RBLO2229-5ppb-2, 2/22/99 TOX 120, RBLO2229-10ppb-2, 2/22/99 TCUI 1 1 0 , aSLO22Z9-z?ipp&2, 2/22/99 TOX 120. BB102229-50&-2, 2/22/99 TOX 120, EBLO2229-lOOppb-2, 2/22/99 TOX l20, -2229-25-2, 2/22/99 TOX 120, RBLO2229-5OOppb-2, 2/22/99 TOX l20, 118102229-75Oppb-2, 2 1 W 9 9 T o r 120, RBL02229-1OOOgpb-2, 2/22/99 5.99 12.00 29.90 59.90 . 4.126 120.00 4.WO 299.00 4.124 599.00 4.143 198.00 4.213 . 1198.00 4.209 . 97 1 212 1 399 1 659 1 1110 1 iiai i 48.06 bb -20 153.71 bb 28 323.86 bb 1 561.92 bb -6 974.76 bb 9 l222.ll bb 2 299.00 4.147 401 1 325.85 bb 9 299.00 4.171 473 1 392.19 bb 31 299.00 4.039 3a9 1 299.00 299.00 4.046 4.390 4.297 4.057 5.99 12-00 29-90 59.90 120.00 299.00 599.00 898.00 ii9a.00 .. 4.247 4.247 4.l59 4.163 4.145 4.129 3139 1 377 1 25s 1 427 1 102 1 176 1 388 1 601 1 1036 1 .yu 1 314.75 bb 5 314.84 bb 5 303.93 bb 195.04 bb 350.10 bb 17 ...&"I. 52.25 bb -13 120.05 bb 0 314.63 bb 5 5oa.60 bb -15 906.92 bb 1 1160.16 bb 3 BACK TO MAIN #Iru 1030199001 2 b30199002 3 030199003 4 030199004 5 030199005 6 030199006 . 7 030199007 e o30199008 9 030199009 10 030199010 11 030199011 12 030199012 13 030199013 14 030199014 15 030199015 16 030199016 17 030199017 18 0301990ie 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 24 030199024 25 030199025 26 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 34 030199034 35 030199035 36 030199036 37 030199037 3e o~oi9903e 39 030199039 40 030199040 41 030199041 42 030199042 43 0301990I3 44 030199044 45 030199045 46 030199016 47 030199047 48 030199048 49 030199049 SO 030199050' I1 030199051 52 030199052 63 030199053 'SI 030199054 -t ?e BhCN Blank BhCN Blank Tox 120, RBLO2229-gzO Blk-1, 2/22/99 Tox 120, RBLO2229-RBLivarBlk-1, 2/22/99 Tor( 120, RBLO2229-5el. 2 / W 9 9 TOx 120, RBLOZUO-lOppb-1, 2/22/99 TOX 120, RBLO2229-25-1, 2/22/99 TOX 120, RBD2229-50-1. 2/22/99 T o x 12P. RBLO227.9-1OOppb-1, 2/22/.99 TOX 120, 18LO2229-250ppb-1, 2/22/99 T o x 120, RBLO2229-5OOppb-1, 2/22/99 TOX 120, -2229-75Oppb-1, 2/22/99 Tosl l20,' RBLO2229-1000ppb1, 2/22/99 XoOH Blank Tox 120 0110. 125142, W22/99 DS f o x 120 Dwo. 126tQ. W22/99 05 f o x l20 DIK). 127142. 2/22/99 M TOX 120 Dwo. l29101. 2/22/99 DZ T a l20 D1K). l30143, 2/22/99 D2 ?OX 120, RBLO2229-2SOp@-CQ, 2/22/99 xoa Blank ToX 120 Dwo. 13lF43, 2/22/99 D2 ,Tor120 DlsQ. 13U43, 2/22/99 D2 Tox 120 DNO. 133F43, 2/22/99 D2 T o r l20 DRO. 13061, 2/22/99 D2 .fox 120 W. 135F01, 2/22/99 02 Tox 120, RBLO2229-2S0ppb-cc2, 2/22/99 HaOIi Blank Tox 120 DNO.. 136143, 2/22/99 D2 Tor( 1 2 0 DSIO. l37F03, 2/22/99 D2 TOX 120 DNO. 138F43, 2/22/99 02 ToX 120 DNO. 139143, 2/22/99 D2 ToX 120 DSIO. 14lF43, 2/22/99 02 TOX 120, RBLO2229-250ppb-CC3, 2/22/99 HaOIi Blank . TOX 120 DHO. l42?03, 2/22/99 D2 Tox'l20 DHO. 143F4, 2/22/99 02 T o r 120, RBLO2229-25Oppb-CC4, 2/22/99 HaOIi Blank TOX 120, i06r-~i-25oppb-11~2,/22/99 TOX 120, 106F41-250ppb-US0, 2/22/99 TOr 120, RBLO2229-2SOpPb-cQ, 2/22/99 MaOH Blank . Tor 120, RBLo222S-H?O Blk-2, 2/22/99 Tox 120, RBLO2229-RBUvarBlk-1, 2/22/99 TOX 120, RBLO2229-5-2, 2/22/99 ?Or 120, RBLO2229-10ppb-2, 2/22/99 T M 120. RBLOZ229-25-2, 2/22/90 TOX 120, RBLO2229-5Oppb-2, 2/22/99 Tor 120, RBLO2229-100pp&2, 2/22/99 TOl 120, RBLO2229-2SOppb-2, 2/22/99 TOX 120, RBLO2229-5OOppb-2, 2/22/99 TOr 120, RBLO2229-750-2, 2/22/99 Tor 120. RBLO2229-loooppb-2, 2/22/99 e t a coao E3 7.008 7.512 5.99 12.00 29.90 59.90 120.00 299.00 599.00 89e.00 ii9e.oo 7.329 7.353 7.35s 7.368 7.352 7.302 7.275 q.349 .- A w u.hat. ' 500 1 COOD nags u).v 92.L6 bb 124 1 20.26 WW 10 1 . 1st 1 337 1 573 1 1654 1 3098 1 5019 1 6230 1 11.94 I94 -1 26.17 lQ4 -12 61.26 WW 2 106.54 WW -11 314.01 WW 5 591.19 bb -1 965.76 bb e 1192.43 bb 0 299.00 299.00 6.805 6. (83 7.310 . 6.9846.m 6.895 * 7.326 6.916 7.346 6.890 424 1 424 1 1518 1 571 1 535 1 459 1 397 1 537 1 1325 1 419 1 77.90 bb n . e e 194 .2e7.96 bb -4 106.08 bb 99.25 bb ec.58 m 72.71 bb 99.69 I94 S0.93 I94 -16 76.91 bb 299.00 7.275 299.00 \ 7.287 299.00 7.292 7.271 7.301 7.311 5.99 Y.00 29-90 19.90 120.00 299.00 599.00 e9e.00 . 1198.00 7.356 7.337 7.352 7.332 7.346 7.339 7.305 7.285 sol 1 284.63 bb -5 . 1588 1 301.41 bb 1 1446 1 1440 1 1461 1 231 274.12 bb 272.91 WW . 276.92 bb -7 , c d 0.92 . ?:. 96 1 220 1 334 1 672 1 1SlS 1 30lS 1 4559 1 6206 1 :\ 14.96 BSt 3e.77 1414 . 60.67 W 125.54 I94 287.44 I4I4 575.32 bb (71.76 bb iie7.eq; bb 25 30 1 5 -4 . -4 -3 -1 BACK TO MAIN Nr4m eman k.plJist T a t 1030199001 w.oH Blank 2 030199002 X d I i Blank 3 030199003 TOX 120, RBL02229-XZO Blk-1, 2/22/99 4 030199004 ?OX 120, RBL02229-RBUvarBlk-1, 2/22/99 5 030199005 TOX 120, R8L02229-5pptil. 2/22/99 6 030199006 TOX 120, RBLO2229-1Opp&l, 2/22/99 7 030199007 TOX 120, RBLO2229-25-1, 2/22/99 . 8 030199008 TOX 120, RBLO2229-50-1, 2/22/99 9 030199009 TOX 120, RBL02229-100ppb-1, 2/22/99 10 030199010 TOX 120, EBI.02229-ZSOppb-1, 2/22/99 11 030199011 TOX 120, RBL02229-5OOppb-1, 2/22/99 12 0301990lZ TOX 120, RBLO2229-75-1, 2/22/99 . 13 030199013 14 030199014 15 0301990U T o r 120, RBI.02229-10-r00~1-, 2/22/99 lbOH Blkk TOJI 120 0110. 125142, 2/22/99 W 16 030199016 TO% 120 DWO. 12624. 2/22/99 % 17 030199017 TOX 120 DMO. l27242, 2/22/99 D5 ' 18 030i99oia 'TOX 120 0110. 129101, 2/22/99 Dt . 19 030199019 20 030199020 21 030199021 22 030199022 TOX 120 0110. 130243. 2/22/99 02 TOX 120, ~ ~ ~ o w 9 - 2 5 0 p p b - c2~/.~ / 9 9 )I.o)I BlMk . TOX 120 DUO. 131243, 2/22/99 D2 . 23 030199023 TOX 120 DWO. 132103, 2/22/99 D2 , 24 030199024 TOX 120 DNO. 133Z4, 2/22/99 DZ 25 030199025 T o n 120 DNO. 13W03, 2/22/99 D2 26 030199026 , TOX l20 DMO. 135103, 2/22/99 02 27 030199027 T o n 120, RBLO2229-25Oppb-EC2, 2/22/99 28 030199028 NOH Blank 29 030199029 ToX 120 DNO. 136243, 2/22/99 D2 30 030199030 TOX 120 DNO.'137?4, 2/22/99 DZ 31 030199031 TOX 120 DUO. 138143, 2/22/99 D2 . 32 030199032 ToX 120 DNO. 139k-W. 2/22/99 02 33 030199033 T o r 120 DNO. 14114, 2/22/99 02 34 030199034 ToX 120, RBLO2229-250ppb-co~2/22/99 35 030199035 W H Blank . 36 030199036 37 030199037 38 030199038 Tor 120 DNO. 142F-W. 2/22/99 W TO^< 120 DNO. 1 4 3 ~ 4 ,2/u/99 n2 TOX 120, BBL02229-250ppb-CC4, 2/22/99 39 030199039 w.oH Blank 40 030199040 TOX 120, 106F-Gl-250ppa-lrs,2/22/99 41 030199041 TOX 120, 106Ydl-250~ll!4D,2/22/99 42 030199Q42 Tax 120, RBLO2229-250ppbCC5, 2/22/99 43 030199043 ' w.oHB1.nlr 44 030199044 TOX 120, RBLOZ229-HZO Blk-2, 2/22/99 45 030199045 TOX 120, RBLO2229-RBLimBlk-1, 2/22/99 46 030199046 %X 120, RBLOZU9-5-2, 2/22/99 47 030199047 ToX 120, RBL02229-10&-2, 2/22/99 4 8 030199048 TOr 120, RBLO2229-ZSppb-2. 2/22/99 49 030199049 SO 030199050 TOX 120, RBLO2229-50-2, 2/22/99 rot 120, aelo2229-rooppb-2, 1/22/99 51 030199051 TOX 120, RBL02229-25Op~b2,2/22/99 52 030199052 T- 120, RBLO2229-5OOppb-2, 2/22/99 53 030199053 TOX 120, BBLO2229-75Oppb-2, 2/22/99 54 030199054 TOX 120, RBLO!2229-1000ppb-2, 2/22/99 v U 5.99 12.00 29.90 59.90 120.00 299.00 599.00 198.00 1198.00 299.00 299.00 d.571 8.549 1.593 - 8.562 8.544 a.uo 8.168 8.149 8.361 8.340 8.599 a. 370 8.553 8.372 8.540 8.552 8.559 299.00 8.352 8.331 8.572 8.568 8.585 8.563 299.00 8.572 8.559 8.568 299.00 8.580 (-560 1.505 5.99 12.00 29.90 59-90 120.00 ' 1.536 299.00 8.550 599.00 8.546 898.00 8.505 ll98.00 6.578 '. 86 1 275 1 372 1 631 1 727 L 311 1 301 1 266 1. 329 1 448 1' 173 1 .; 281 1 445 1 260 1 352 1 500 1 163 1 391 1 380 1 408 1 . 357 1 328 1 262 1 257 1 218 1 222 1 226 1 196 1 190 1 119 1 209 1 393 1 . 535 1 .-- 703 1 85.14 M -29 419.51 bbX 40 592.43 lM -1 1049.49 t44X 17 1220.49 bb 2 483.'65 mf 465.17 MK 403.94 bb 514.84'bb 725.79 M 238.58 )PI , -20 430.52 bb 720.90 M 393.56 bb 556.19 ?M 818.12 bb 220.94 M4 625.67 IhI 605.50 bb 654.85 w 564.48 bb 513.91 bb 397.34 nn 388.50 IM 319.39 bb 325.79 EM -26 - 133 ? . 9 333.82 IM 279.87 IM 268.61 IM -10 . I i T. 144.01 bb 20 302.82 bb 1 628.62 Ml 5 879.50 bb -2 1177.99 bb -2 I .. .. prvnurrbqorndAuyR.port zOXl20 DNO Rat 2.i- aoups 2 L 3 S q l q List: Y : \ I M T I . P R O \ ~ ~ \ 0 3 0 ~ 0 0 lut &id mma y.r 08 09:4s:2c 1999 MDthod: Y:b L . . P R O \ y . ~ \ 0 3 0 0 a k s t a d :Ibn llrr 00 10:03:52 1999 Job cod.: Priatd: Yoa mr oa 10:U:U l s 0 0 copanrd c: T d m u 5 6 B #- lupLJ1.t T . r t 1030199001 Blank 2 030199002 3 030199003 nm0R Blank T o r 120, RBLO2229-HZO Blk-1, 2/22/99 4 030199004 T o r 120, BBL02229-RBLiverBlk-1, 2/22/99 S 030199005 tor 120, m~0uz9-sppb-1, ~ / t z m 6 030199006 TOX 120, nslo2229-10-1. 2/22/99 7 030199007 tor 120, RBLO2229-2SpPa-1, 2/22/99 8 030199008 T o r 120, RBLO2229-50-1, 2/22/99 9 030199009 TOX U O , RBLO2229-100ppb-1, 2/23/99 10 030199010 Tor 120, llBLO2229-25Oppb-1, 2/22/9Y 11 030199011 TOX 120, nslo2229-500ppbl. 2/22/99 12 030199012 T o r 120, RBLO2229-75OpPa-1. 2/22/99 13 030199013 T o r l20, RBL02229-1OOOppb-l,~2/22/99 14 030199014 15 030199015 MoOM Illmk TOX 120 m.~ 2 5 ~ 2~/252/9, 9 m 16 030199016 17 030199017 T o r 120 DD1Q. l26F-GZ. 2/22/99 Ds for 120 m.I~'ROZ, 2/22/99 m 18 030199018 TOX 120 DUO. l29F05, 2/22/99 D2 19 030199019 T o r 120 DM). 130F05, 2/22/99 D2 20 030199020 T a 120, RBLO2229-250ppb-ccI. 2/22/99 21 030199021 22 030199022 It.oDI Blank Tor 120 m. 131101, 2/22/99 D2 '23 030199023 TOX 120 0310. 132.05, 2/22/99 D2 . 24 030199024 25 030199025 26 030199026 27 030199027 T o r l20 DUO. 133905, 2/22/99 02 T o r 120 Lore. 134P43, 2/22/99 02 TOX 120 0310. USPQ3, 2/22/99 D2 T o r 120, RBLO2229-250ppb-ccz, 2/22/99 28 030199028 &OH Blank 29 030199029 T o r l20 DPIO. 136F05, 2/22/99 D2 30 030199030 31 030199031 T o r 120 DNO. 137945. 2/22/99 D2 Tor 120 DHO. 138F43, 2/22/99 D2 32 030199032 TOJZ120 0310. 139943. 2/22/99 D2 33 030199033 Tax l20 DNO. l4lF43, 2/22/99 D2 34 030199034 TOX 120, RBL02229-25O&-CU, 2/22/99 35 030199035 Wll Blank 36 030199036 Tor 120 DUO. 142943, 2/22/99 D2 37 030199037 . T o k l20 0310. 143F-05, 2/22/99 02 38 030199038 T o r 120, RBLo2229-250pp&ccI, 2/22/99 39 030199039 w.011 Blank 40 030199040 TO%120, 106F-G1-25Op+lS, 2/22/99 41 030199041 TO% 1 2 0 , 106F-Gl-25O~lS0, 2/22/99 .42 030199042 T o r U O , RBLO2229-2SOPpb-EQ. 2/22/99 43 030199043 XeW Blank 44 030199044 TOX l20, RBLO2229-820 Blk-2, 2/22/99 45 030199045 TOX 120, IIBLo2229-RBuWrBlk-1, 2/22/99 46 030199046 Tor 120, RBLO2229-5-2, 2/22/99 47 030199047 TOX 120. RBLO2229-10ppb-t, 2/22/99 4 8 050199048 Tor 120. IBLO2229-25-2. 2/22/99 19 030199049 TOX 120, :9BLO2229-5Oppb-2, 2/22/99 50 030199050 TOX 120, RBLO2229-100ppb-2, 2/22/99 51 030199051 TOR 1 2 0 , RBLO2229-25OPpb-2, 2/22/99 52 030199052 TOX 120, RBLO2229-500-2, 2/22/99 53 030199053 TOX l 2 0 , RBLO2229-750ppb2, 2/22/99 54 030199054 T o r 120, RBLO2229-1OOOppb-2, 2/22/99 BACK TO MAIN Aru DLl.hot. 5.99 12.00 29.90 59-90 9.414 l20.00 9.429 299.00 9.414 599.00 9.460 aoe.00 1198.00 - 9.424 9.401 117 1 238 1 528 1 896 1 1478 1 1532 1 ' 58.83 bb -2 111.81 bb -7 238.62 bbX -20 399.67 bbx -33 654.21 b b ~ -27 677.92 bb* -43 299.00 9.469 9.419 299.00 9.422 354 1 105 1 507 1 299.00 9.428 9.444 9.427 299.00 299.00 9.420 9.429 9.443 9.427 9.450 56 1 49 1 481 1 48 1 541 1 448 1 357 1 337 1 stmnia stud.. s+ud. stud. Stud. StU& Stud. stud. 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1190.00 9.407 9.431 9.413 9.426 9.414 9.454 9.440 64 1 . 132 1 242 1 676 1 896 1 .:\ 1154 1 1729 1 162.79'bb -46 53.86 bb 229.52 l44 -23 32.09 lk 29.38 Hd 2 1 ~ 0 sbb -27 . 20.51 Hd 24S.83 bb -18 203.94 bb 163.92 l44 155.01 bb 1 ,111 f? . -48 ' c 36.56 l44 19 65.50 bb 9 113.63 bb -5 3v3.37 bb 1 399:62 bbx -33 512.54 bhx -43 764.09 bbX -36 I I i I 1 ! I 1 3 7 1 f 1 I I I 1 3 Rined: yoa yrt 08 1o:u:49 1099 bpouad 7: mlrz 0 luw 1030199001 2 030199002 3 030199003 4 030199004 5 030199005 6 030199006 7 030199007 8 030199008 . 9 030199009 10 030199010 11 030199011 12 030199012 13 030199013 14 030199014 15 030199015 16 030199016 11 030199017 ia 0301990ia 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 24 030199024 25 030199025 26 030199026 27 030199027 28 03019902a 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 34 030199034 35 030199035 36 030199036 37 030199037 38 03019903a 39 030199039 40 030199010 41 030199041 42 030199012 43 030199013 44 030199044 45 030199045 46 030199016 47 030199047 48 030199048 49.030199049 so 030199osa 51 030199051 52 030199052 53 030199053 54 030199054 w aU s t T u t x8oH 1Ilmk II.ooT Blu* T o r 1.220, FSL02229-XZO Blk-1, 2/22/99 T o r 1 0, RBLO2229-RBLivorBlk-1, 2/22/99 T o x 1.20, -2229-5p&-1, 2/22/99 Tor 1.20, RELO2229-10ppb-1, 2/22/99 T o x 1.20, RBLO2229-2Sppb-1, 2/22/99 T o r 1 0, RBLO2229-5Oppb-1, 2/22/99 T o r 1.20, RBLO2229-100ppb-1, 2/22/.99 Tor 1.20, RBLO2229-UOppb-1, 2/22/99 T o x 1.20, RBLO2229-5OOppb-1, 2/22/99 . T o r 1.20, RBLO2229-7SOppb-1, 2/22/99 Tor 1.20, RBLO2229-lOOOpp?-l, 2/22/99 HooH BU d C Tor 1.2o mo. isr-cz, 2 1 ~ 9 ~95 T o r 1.20 DIK). 1 2 6 1 0 2 . 2/22/99 D5 T o r 130 DD10. l2l?-GZ, 2/22/99 D5 Tor 1.20 DNO. 129?U, 2/22/99 D2 Tox 1 o mo. iior-~, 2/2w99 a f o x 1.20, RBLo2229-250ppb-Cc1, 2/22/99 HooH B lank Tor 13o DNO. u i r u , 2/22/99 02 T o r 1.20 PWD. l32?U. 2/22/99 D2 ToX 1.20 DNO. 133143; 2/22/99 D2 T o r 1.20 DNO. 134243. 2/22/99 D2 .Tax 12 0 DNO. 135?U, 2/22/99 D2 T o r 1.20, RBLO2229-250ppb-QQ, 2/22/99 mon BIluIk ToX 1.20 DNO..l362-Q, 2/22/99 D2 Tox 1.20 mr0. l372U. 2/22/99 D2 T o x 1.20 DUO. U82-Q. 2/22/99 D2 T o x 1.20 mr0. 139F43, 2/22/99 D2 T o r 1..220 DNO. 141F43, 2/22/99 D2 Tor 1 0, RBLO2229-250ppb-cp. 2/22/99 HooH Blluk .tor 1.20 DNO. 142I-Q. 2/22/99 D2 Tor Y.20 DNO. 143F-Q. 2/22/99 D2 Tor 130, EBLO2229-25Oppb-eU. 2/22/99 &OH Bllank Tor 130, 1 0 6 F O I - 2 5 O ~ W ,2/22/99 Tor 1-.210, 1061-01-25O&-HSD, 2/22/99 T o r 1 10, RBLO2229-250ppb-cQ, 2/22/99 mi BIlmL Tor 1310. RBLo2229-Ioo Blk-2, 2/22/99 Tor I.z!O. RBLO2229-RBLivarBlk-1, 2/22/99 Tar 13IO, EBLO2229-Sppb-2, 2/22/99 T o r 1.210. 3LO2229-1Oppb-2, 2/22/99 TO* 12!O. EBLO2229-25ppb-2, 2/22/99 Tox I.Z!O, RBLO222Y-5Oppb-2, 2/22/90 Tor Irl!O, RBLO2229-100-2, 2fUl99 Tor ILa!O, EBLO2229-UOppb-2, 2/22/99 Tor 1Li!O, BBLOW9-SOOppb-2, 2/22/99 Tox Iu!O, RBLO2229-75Oppb-2, 2/22/99 Tor 11; !O, BBLO2229-lOOOppb-2, 2/22/99 .I 1 I 1 I 1 u d r t c a r Q u o 9 8 : : wutatd .nd prhrwl at/08/S9 by drb ---- std cam 1.00 1.00 1.00 1-00 1.00 1.00 1.00 1.00 a.00 7.334 7.344 7.275 7.277 7.302 7.303 7.315 7.299 7.249 7.308 7.297 494 1 579 1 1017 1 1248 1 1111 1 . ,1149 1 1240 1 1233 1 1192 1 l245 1 11171' . 1.00 7 . m 908 1 1.00 7.293 1063 1 7.245 1.00 491 1 1.00 1.00 1-00 1.00 1.00 1-00 1.00 1.00 1.00 1.00 1.00 1.275 7.284 7.302 7.286 7.300 7.2~1 7.295 7.287 7.252 7.2M 1010 1 1172 1 un 1 1293 1 1141 1 rioa i w41 1 3198 1 958 1 ,1229 1 :\ , BACK TO MAIN 0.41 m -59 0.48 1pI -52 0.85 IM -15 1.04 M4 4 0.92 M -8 0.95 m -5 1.03 M 3 1-02 m ' 2 0.99 IQI -1 1.03 IQI 3 0.93 1DI -7 0.75 lQ4 -25 0.88 JQ4 -12 0.41 JQ4 -59 . .5. 0.84 & -16 0.97 tbr -3 1-14 11 1.07 IQI I 0.95 rn -5 1.17 la4 17 1-11 m ll 1.00 m 0 0.80 IM -20 1.02 IQI 2 Cdibratian: Y : L a t s . ~ \ ~ \ o l o l s s I l h8t m d i f i d : yo lluc 08 10:10:07 la99 Aintd: Lbn b h r 08 10:JI:W US9 bmpound 1 name: PFOS brrelation coefhcient r = 0.997569, f'2 = 0.995144 * hlibration curve: 37.6344 * x + 1719.03 esponse type: Extemal Std, Atea hmetype: Linear, Origin:Exdude,Weighting.Null, Aris trans: None 4.88e4 .. BACK TO MAIN Pap. 1 X Response n d d h m O U O 9 8 : : quankitatd .rd 400.0 03/08/99 by drb 600.0 800.0 1000.0 Conc - .. BACK TO MAIN - 1 . : ~ hmpound 2 name: PFHS + * I &relationcoeffident:r = 0.999248, d'2 7 0.998498 Uibrationcurve: 205223 x 973922 lesponse type: Extemal Std, Area i hrve type Linear, Origin:Exclude,Weighting Null, Axis trans:None 1 2.48e4 1 1 1 Response 1 i 1 < (I i . 248, ,, ,, , , , , , ;, , , , , , I , , , " I , , , , I , , ,; .. 1 1 1 /- YoConc I , , ;,, , , I , , , , , , , , , I f ( I X X 1 X t J -5.04 ( 1 I 200.0 400.0 600.0 800.0 1000.0 Conc ! 1 1 1 I I 1 1 1 .1 I I 1 J 3 1 I J .I 1 I - .- ~ bmpound 3 name: brrelation coefficient r = 0.995235,1'2 = 0.990494 ' klibrationc~~lve1.:09344* x + 44.3843 ksponse tppe:Extemal Std,Area :urve qpc =ear, Origin:Exdude, Weighting Null, Axis trans: None Respons udrLirrr041099:: ~ U t r t add printd 01/09/09 drb BACK TO MAIN ->:. . X 1 - . .. . 1 -ti-: Y:bats.pRO\~\O3099a l u t M W . Yon Bhr 08 10:10:07 U S 9 Rind yu: 08 1O:lU:OS US9 - ompound 4 name: P O M 1 orvelationcoefticient:1:= 0.998817,62 = 0.997636 ' alibrationcurve:5.20938* x + 18.1144 esponse type: External S t d , h 1 urve type: Linear, Origin:Exdude, Weighting Null, Axis trans: None 1 6.266 1 .. '1 I I I i I1 Response 1 1 1 I I .I f 200.0 400.0 600.0 .1 ---- ~ 0 U 0 9 8 : :q m b t i t . t d a d p r h t d 0~/08/9S by drb . 800.0 ! BACK TO MAIN 1ouo.u ompound 5 name: Tekner469 . arrelationc o e ~ e ntt= 0.998530,r"2 = 0.997062 ' albration curve: 0.565020 * x + 37.7497 esponse type: Extemal Std,Area urn type: Gear, Origin:Exclude, Weighting:Null, Axis trans: None 727 BACK TO MAIN Rap. I X - _ . .. ompound 6name: Telmer569 orrelationc o m e r = 0.997982,f'2 = 0.995968 dibrationcurve: 228567 * r +-17.6540 .esponsetype: Eaemal Sd, Area hrve type: Linear,Otigin:Exdude, Weighting: Null, Axis trans: None 2.72e3 BACK TO MAIN .. *- Responsc J -17.: .m" ?- .*\ :ompound 7 name: sua Lsponse Factoc 1203.87 Lesponse type: Extemal Std, Atea hrve type: RF BACK TO MAIN X X X X , , , ; I Cone I 0.1 0.2 0.3 0.4 0.5 . 0.6 0.7 0.8 0.9 1.0 1 1 ! Printad: Colpound n u : Har N.r 08 10:10:02 1999 PIQI fntarnal Raf. : Quantify Trua: Maul TIC 1 . bnc. of stadud8: U a u 1v Valur: Uaar P o d ?actor: Cam. A 1.000000, 1.000000 Dimblad Intagrat. W i n d a Zaetor: 2.000 1 Paak Loution Ratantion Tk. (rClu): b h t i v a ato on ti on Tiw: 8.130, m a d . 0.000, Diaablad Tk.Wind#(rinrr): * 0.793 f Paak Salaction: Uoawnaa I.uut h-80 -8 I Raapaua: Calibration Cunna Polynomial Typa: Point of Origin: 1 Z i t Waighting: Axia Trrwforution: Concantration LiMu Kxeludo nnau Uni+r: I Noh. I - PHk-to-pak wlitud.: ' 150 - Smooth . f smoothing: W i n d c u sit. (.una) : Humbar of aaootha: s ~ h h Bgathod: Malbld 3 2 I Barrlinaa Join valloy thraaholdr Raducr paak tailing: Ilriaa bualina . i x k m r ~ 20.00 100.00 0.00 J Paak Sauuation Drm+ varticrl tluarhold: D a t e Dhculdara: Shouldor thraahold: !I RmaDonaa Thraahold hlativa high+: Ab8oluta bight: Rmlativa ura: I Ab8oluta u a a t 10.00 Di8.bl.d 10.00 . 1.50, D i 8 . b l . d 0.00, D b a b l d 2.00,. Dirabld 80.00, .m a. d I 1 1 I BACK TO MAIN .. .-_ .. - .' .. .. .. .. .. -..'k 1 Hothod Raport Hothod f i l a : lut r o d i f i d : I:\rata.PROUI.tMB\03oi99c.mdb Won X.r 08 10:03:52 1 9 9 ~ c=Poud: 2: P m s Print* Xonlur 08 1O:IO:OZ 1999 ~olpoundn-: Int' Rof.: Quantify T r u a r cons. of srud.rdar Uaar U Valw: Uaar Poak lactor: Intagrato WMOU Iactce: Paak Locatia Wontim T i m ( m i d : Rolaticn Rotantion T h e : TL.Wkda,(~inal: * t a d Salutim: . Roarunro ims . IH-1 TIC Cuts. A 1.000000, Mubld 1.000000 2.000 1.732, R v b r d 0.000, Dimb1.d 1.180 Maw+ C a l i k a t i m Cumas P o l y a a l r l Typat Paint of Origin: lit Uaightinqz Uri. Truuforutiat: Concantratim . Unit.: - nois. Pmak-tepaak mittd.: - Smooth lGuu -1- HaU Mru 290 R m m a Thrashold Ralativa b i g h t : Abaoluto b i g h t s Ralativa :.u Abaoluta uaa: 60.00 100.00 0.00 10.00 DLab1.d 40.00 1-50, Diaabld 0.00, Msabld 2.00,' D h 8 b l m d 80.00, mubld - .. IN. wr 048 BACK TO MAIN --- - Paga 2 .. a .. =.- . I 1 Method Report 1 Method f i l a : Laat mcdifhd: Empound: U:\rata.PROUIetMB\o~oi99c..db Hon U u 08 10:03:52 19990 3: I Printed: colpaud wame: InteMel Ref.: lbn f i r 08 10:10:02 1999 tIlaf.1 Quantify'Trua: TIC I cart. of Sturd.rd.2 U a u Iv Value: Uaer P m k rutor: Cake. A 1.000000, -.bled 1.000b00 Integrate Uinda, ?actor: 2.000 1 Pmk Location Retention rL. (Bins): R.1ati-m Retention T k . : T k . y i n d # (&): ' 4.183, W e d 0.000, Diaabld 1.000 3 Peak IalectSon: Reamae IOU& Rempaue T y p : 1 pa leer c.libration Rwu P0lynari.l Type: point of origin: T Z i t Weighting: kir buuforrutiaa: Concantration taulul ' . Areu e. .. &itJS 1 - Nofae Pe.k-to-p..L amplitude: 169 i 1 i J '1 1 I I s.oothinp: Windor a i m (.canal:. Yurb.r of a m : $moothing rthod: Dhbled 3 2 luelinea Join velley threahord: Reduce peak tailing: R8iae bueline m U i B u I u * 20.00 100.00 0.00 Peak SeMration Drw v r r t i u l thrrsbldr 10.00 Detect aharldera: I Diaabled Shsder threa-. 40.00 * Reamae Throahold Relativa height: Absolute height: Llative uu: Absolute ua.: 1.5.0, Diaabled * 0.00, D i a M 2.00, * D i s a b l e d 80.00. h.bled I 1 1 1 I. . .. BACK TO MAIN ~- Page 3 .. .* . . r-. - -.. BACK TO MAIN . 1 I i J 1 Pbak Sbwrmtion Drbu V b r t t G d -hold: 10.00 btoct a h d d o a r Dirablod Shouldar thmiyd; , (0.00 9 RbL.sDOlWb'Thruhold Rblativm b i g h t : 1-50, D i w b l d -1 Ab.OlUtb bight: Rblativb arb.: AhrO1Ut.b ' O.OO,.DL.bld 2.00, D L . b l d 00.00, Di8.bld .- I J 1 i .. I hthod bport h t h o d fib: Laat rodifid. Capound: Printed: n:\rata. Pno~thrm\o30199e.~db Xon W.r OB 10:03:52 19990 5: Tbhbr469 I(nr W.r OB 10:40:02 199) c4lpound w-: XlltiMd Uf.: Quantify Tracb: canc.ofstududo: . . Uaar Iv V d w : Uabr Pbak ?&Or: Intagrat. W k d o u reor: Pbak location - Tbhr169 mm.1 TIC Cac. A 1.000000, D i m b l e d 1.000000 2.000 169 , adativebight: Ab.OlUtb bight: U l a t i v a mr: Abaolut. uu: 20.00 100.00 0.00 10.00 Dir8bld I 40.00 1.50, Mambld 0.00, D i J a b l d 2.00; Dia.bibd 10.00. s8Ubl.d .. BACK TO MAIN Pap. 5 .. Jlathod Report Printdr Mab Kar 08 10:40:02 1999 1I C a P o U n d Y u r l I n t a n u l Rof.: puurtify Traca: conc. of studud.: U a u RF Vdua: 1 U a u Paak ?actor: Intagrat. W i n d m Ieor: TaL.rS69 1n0n.1 TIC C~IC. A 1.000000, D L a b 1 . d 1.000000 2.000 I Paak location Rotantion TL. Wnr): Rolativa Ratantian T h r ?L.lfindcw(rinr)r * 9.416, 0.000, 0.500 Bubld Diaabld I '1 . 1' I 1 1 1 1 Join v r l l a y thraahold: M h u c a paak iaa buali - taillng: n a nximm. . Paak Soparatian 20.00 100.00 0.00 Dr- vrrtieal thruholddr -tact ahouldarai Shouldor thrashold: , 10.00 . Diaablad 40.00 RaaWnaa Thraahold hlativm haight: Absoluta haightr Rol.tlvm :.u Ab.olut. UN: 1.60, D b a b l d ' 0.00, *&lad 2.00,.DLablad , 80.00, h.bld 1 1 I' BACK TO MAIN Paga 6 Method Wport Method f i l e : Laat modifiad: conwynd: Printd: II:\rata.PRD\X.thDS\030199c.db Xon X u 0 1 10:03:52 199- 7: murr non Mar 08 10:10:02 1999 compound n.u: Intarnal Rmf.: Quantify Trace: cone. of Studerda: Uaor RI Valuo: U s u Peak Pactor: ' Integrate W L d a r Pactor: Poak location RmtontSan T i n a WM): Rolatiw Retantiaa Tho: T h l i i n d a a WM): * PO& se1eCtioo: Rmauonm. auiuma. T y p a mpcnaer Crlibratia QImr Polynomial Typo: point Of origin: ' rit weighting: M J Trruuf-ti?: Concentration Unit.: a P.ak-to--p.ak - Smooth UplitUa.: SIOothing: Windat size (acana): 19umborof-r ' smoothing utw Baaelinea Join valloy thsoahold: Rduco p a k +.ilk Raise h e l i n e Puinm:. Peak Semratim D r u V r r t Z C r l throsboldr -+.et ahouldora: I Shouldu tbmholdr kawmae Threshold ' RolatlW b i g h t : Aholute hdght: RAlativm uea: AhYolut. uoa: anrr male1 TIC Ikd 1.000000, D h b l d 1.000000 2.000 7.300. R u b l d 0.000. D b A l d 1.000 muut 169 DiSrbld 3 2 20.00 100.00 0.00 LO. 00 Di..bld 10.00 LSO, Dia.bld 0.00. Diaabld 2.00, Dia.bld 80.00, -1d - .- BACK TO MAIN . . _.-. . C.. Page 7 IAL 03/081/Pq .. BACK TO MAIN 1 - .. --.._- . AMELIA062498: FACT Equipment Log 98061 Quattro II,#6448E PAGE ANALYrnhOj . STUDY NUMBER toxl20. MA- Rat liver DESCRW?ON: ion pair dilutions FACT Method: FArn-M-4.0 Injection volumc,lO uL Cycletime: 13.5min. Eltctrodc: %Spray Mobile Phase: (A)2.0 mM NH4CH3CO2 TN-A- or stndrdnumber (A).98094-15-20' prcpann (&MY): (A) 3/16/99 hoj (B)M&H ' . (B)TN-A-2325 (B) 3/16/99 hoj EIcpirm . (A)09M Storeatroomtemp? fl or N other: (B) 9/16/99 Guardcolumn: None Analytical column: keystone betasil size: 2 x loomm T G :c18 sphaesize:5um Part numb= 105-701-2 'Lotnumb= P8H08 Serialnumb= 0294951) I halyticalparame~. HPLC solvent gradient: long2 Time (min.) %B 1) 0.00 40 2) 8.5 90 - 3 11.0 90 5) 12.5 40 6) 13.5 40 1 1 Tuning Method Report Method: C:\MAsSLYNX\RATS2.PRO\ACQUDB\PFOS 1 Printed: ' Tue M a r 1 6 09:59:43 1999 I 1 3 1 BACK TO MAIN ' Page 1 I AC .. . i I I Ms I SOURCE ( ESP- ) Capillary Cone 1 Extractor RF Lens Source Block Temp. Desolvation Temp: -1 1 Pressures 1 Analyser Vacuum Gas Cell I I .i I' ! Dau 499.00 Set 3.00 60 5 0.2 150 ,250 Rdbk. 2.99 -60 -6 150 258 Rdbk 1.Oe-4 4.5e-3 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier :\ Ms2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier S e t Rdbk 15.0 15.0, . 2.0% 0.0 5 6 650 S e t Rdbk 15.0 15.0. 45 46 2.0 . 0.0 4 0 38 0 46 650 BACK TO MAIN I Inter Cl1-1 Dday ( S e a I : 0.03 Spur ( D a l t O M 8 0.00 Start T l m I Xins b : 0.00 UdTfu(Wina) I 13.50 nepeat. I 1 chrrwl Parmt Daughter -11 (Seal Coll tnugy (*VI Colu V 1 1 1 499.00 80.00 0.40 45 60 2 499.00 99.00 0.40 ' 45 60 3 499.00 130.00 0.40 45 . , 60 4 499.00 180.00 0.40 4s 60 Inter C l u n ~ 1Delay t S e a 1 t 0.03 Span 1 Dalton. 1 : 0.00 . - Start T W ( !UM 1 I 0.00 * . . Ind T i r u L IUns I : 13.50 .. napeats : 1 . . Ch-1 Parent Dauphter Dwell [sou) C o l l tnerqy,(eV) ,Con. ( V 1 . 1 ' 2 '1 .. 3 4 399.00 '$0.00 399.00 99.00 199.00 130.00 399.00 180.00 0.40 0.40 0.40 ' 0.40 45 . 4s 45 45 ' 60 60 60 60 nlnotionI3 ##al#.eNr(uP-) 1 Inter Channel -lay ( S e a 1 I Spur t Dalton. 1 I . Start tim t M i n i 1 : ( a M )I Repeats : 0un11.1 Pumt a u @ t i r 0.03 0.00 0.00 13.50 1 -11 . [ s e a ) cou mergy (*VI eone ( v I 1 427.00 00.00' 0.40 40 60 .. 3 .. .. .. .. f .--.>- I I - Masslynx Sample Ust Sample List C:\MASSLYNX\rats2.PRO\SampleDB\O31699b.SPL Printed: Tue Mar 16 14:40:11 1999 BACK TO MAIN _. 2 -..Page 1 1 2 3 4 5 6 7 8 9 10 I1 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 . 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 58 57 58 59 60 Flle Name 031699001 031699002 031699003 031699004 031699005 031699006 031699007 031699008 031699009 031699010 031699011 031699012 031699013 031699014 031699015 031699016 031699017 031699018 031699019 031699020 031699021 031699022 031699023 031699024 031699025 031699026 031699027 031699028 031699029 031699030 031699031 031699032 031699033 031699034 031699035 031699036 031699037 031699038 031699039 031699040 031699041 031699042 031699043 0316 9 W 031699045 031699046 031699047 031699048 031699049 031699050 031699051 031699052 031699053 031699054 031699055 031699056 031699057 031699058 031699059 031699060 File Text MS Flle MeOH Blank MeOH Blank T0~120,RBL02229-H20Elk-2,2/22/99 T0~120,RBLO2229-uVBlk-2,2/22/99 Tox120, RBLO2229-5ppb-2,2/22/99 Tox120, RBLO2229-lOppb-2,2/22/99 T0~120,RBLO2229-25ppb-2,2/22/99 T0x120, RBLO2229-5Oppb-2,2/22/99 Tox120, RBLO2229-1OOppb-2,2/22/99 Tox120, RBLO2229-25Oppb-2,2122/99 Tox120, RBLO2229-5OOppb-2,2/22/99 Tox120, RBLO2229-75Oppb-2,2/22/99 * Tox120, RBLO2229-100Oppb-2,2/22/99 MeOH Blank Tox120,161F-G5,2/22/99, d250 Tox120,162F-G5,2122/99, d250 T0~120,163F-G5,2/22/99, d250 Toxl20,164F-G5,2/22/99, d250 Tox120,165F-G5,2/22/99, d250 T0~120,RBLO2229-25Oppb-CC2,2/22/99 MeOH Blank T0~120,166F-G5,2/22/99, d250 T0~120,167F-G5,2/22/99, d250 T0~120,168F-G5,2/22/99, d250 T0~120,RBL02229-250ppb-CC3.2/22/99 MeOH Blank T0~120,177F-G6,2/22/99,d1000 . T0~1.20,1787F-G6,2/22/99,dlOOO T0~120,179F-G6,2/22/99, dlOOO T0~120,180F-G6,2/22/99, d1000 T0~120,181F-G6,2/22/99, dlOOO T0~120,RBL02229-250ppb-CC4,2/22199 MeOH Blank Tox120,182F-G6,2/22/99, dlOOO Toxl20,183F-G6, 2/22/99, dlOOO T0~120,184F-G6,2/22/99, d l000 T0x120,185F-G6,2/22/99. dl000 Tox120,186F-G6,2/22/99, dlOOO 10x120, RBLO2229-25Oppb-Cc5,2/22/99 MeOH Blank T0~120,187F-G6,2/22/99, dlWO Toxl20,189F-G6,2/22/99, dlOOO Tox120,19OF-G6, 2/22/99, dlOW T0~120,191F-G6,2/22/99, dl000 Tox120, RBLO2229-25Oppb-CC6,2/22/99 MeOH Blank Toxl20.169F-G5,2122199 . T0~120.17OF-G5,2/22/99 Toxl20.171F-G5,2/22199 T0~120,RBLO2229-25Oppb-CC5,2/22/99 MeOH Blank T0~120.173F-G5,2/22/99 10~120.174F-G5,2122199 T0~120.175F-G5,2/22/99 Tox120, RBLO2229-25Oppb-CC6,2122/99 MeOH Blank T0~120,RBL02229-H2081k-2,2/22/99 T0~120,RBLO2229-uvBlk-2,2/22/99 .TOXl20. RBL02229-5ppb-2,2/22/99 Tox120, RBL02229-1Oppb2,2/22/99 PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOSPFHS-IS PFOS-PFHS-IS PFOSPFHS-IS PFOSPFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOSPFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHSIS PFOS-PFHSIS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHSIS PFOS-PFHS-IS PFOS-PFHS-IS PFOSPFHS-IS PFOSPFHS-IS PFOS-PFHS-IS PFOS-PMS-IS PFOS-PFHS-IS PFOSPFHS-IS PFQS-PFHS-IS . PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHSIS PFOS-PFHS-IS . PFOS-PFHS-IS' PFOS-PFHS-IS PFOS-PFHS-IS PFOSFFHS-IS ' PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOSPFHS-IS PFOSPFHS-IS PFOSPFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS Inlet File long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 bngz long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 . long2 long2 long2 long2 long2 lone2 long2 long2 long2 long2 long2 bng2 bng2 low bng2 bng2 long2 long2 long2 long2 long2 long2 bng2 long2 . long2 Bottle 91 91 1 2 3 4 5 6 7 8 9 10 11 91 12 13 14 15 16 82 91 17 18 19 83 92 20 21 22 23 24 84 92 25 26: . 27 28 29 85 92 30 31 32 33 86 93 34 35 36 . 85 94 37 38 39 86 94 1 2 3 4 Volume Sam 10 AnaE 10 Anal 10 Anal 10 Anal 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Anal ' 10 Anal 10 Anal 10 Anal 10 Anal 10 Anal 10 QC 10. Anal 10 Anal 10 Anal 10 Anal 10 QC 10 Anal . 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana 10 QC 10 Ana ' 10' h a 10 Ana . l o Ana .10 Ana 10 Ana 10 QC 10 Ana 10 Anz 10 Ans 10 An 10 An 10 QC 10 Arx 10 Ani 10 Ani 10 A n 10 QC 10 A n 10 Ani 10 Ani 10 Ani 10 QC 10 Ani 10 An; 10 An1 10 st 10 St Sample List C:\MASSLYNX\ratts2.PRO\SampleDBW31699b.SPL Printed: Tue Mar 16 14:40:11 1999 File Name 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067 File Text Toxl20, RBL02229-25ppb-2,2/22/99 Toxl20, RBL02229#ppb-2,2/22/99 Tox120, RBLO2229-100ppb-2,2/22/99 Tox120, RBL02229-25Oppb-2,2/22/99 Tox120, RBLO2229-5OOppb-2,2/22/99 Tox120, RBLO2229-75Oppb-2,2/22/99 Tox120, RBLO2229-1000ppb-2,2/22/99 MS File PFOS-PFHSIS PFOSPFHSIS PFOSPFHSIS PFOS-PFHS-IS PFOS-PFHSIS PFOS-PFHS-IS PFOSPFHS-IS BACK TO MAIN Bottle 5 6 7 8 9 10 11 Volume Sam 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 1 I .I .*\ 1 I Date: Analyst: 1 FACTS~LI&N& 1 Matrix 1 3 1 1 I i I 1 1 _I I -1 J FACT: DATA REVIEW SUMMARY FORM .. 3117h4 LAC - T0)C t 20 I I 1. . I I BACK TO MAIN -\ 4 I s w . U a t r 0:\nt82.~\~.D.\MiSSOb b a t m u d i f i d : Id Ilu 17 U:54:lO 1999 Mathod: O:\r8t82.PI0~~\031699b h a t lodifid: U d Ilu 17 06:56:20 1999 Job Cod.: Rintd: Hd 17 14:33:58 1999 1: PIOI i 031699001 2 031699002 3 031699003 ..ql&i.t 2.rt KaOrt Blank M a o n Blank TOXl20, RBLO2229-BZdk-2, 2/22/99 4 031699004 T0xl2Or RBL02229-llTBlb2, 2/22/99 5 031699005 TOX120, 118102229-*2, 2/22/99 6 031699006 TOx120, RBL02229-10peb-2, 2/22/99 7 031699007 ToXl20, RBLO2229-25ppb-2, 2/22/99 0 031699000 Tox120, RBLOZZZS-5-2. 2/22/99 9 031699009 ~0x120, ~~~02229-10~0bpti/2z,2/99 10 031699010 Tox120, RBLO2229-25Oppb-2, 2/22/99 11 031699011 TOX12O~'W2229-50O~~b-22./22/99 12 031699012 13 031699013 14 031699014 15 031699015 naon ~ i a n k TOX120, 16lT-GI. 2/22/99, a 5 0 16 031699016 hXl201 162T-G5, 2/22/99, . a 5 0 ' 17 031699017 zOxl20, 1631-65, 2/22/99, d250 1-~0 03.1_ 6.9.9.01.1._.TOX120, l 6 4 W , 2/22/99, d250 ~ 19 031699019 TOX120, 165FW. 2/22/99, d250 20 031699020 21 031699021 22 031699022 TOxl201 RBLO2229-25oppMQ, 2/22/99 Mmow Blank ToX120, 1661-W. 2/22/99, d250 23 031699023 24 031699024 TOXl20, 1671&, 2/22/99, 6250 Toxl20, 1611-6S. 2/22/99, d250 25 031699025 26 031699026 27 031699027 T0~120,'W2229-250ppb-CU, 2/22/99 MOHBlank 0 tOXl20,' 1771-06, 2/22/99, dl000 28 031699020 TOXl20, 178-6, 2/22/99. dl000 29 031699029 TOXl20, 179FG6r 2/22/99, dl000 30 031699030 TOXl20, 1101-66, 2/22/99, dlOOO 31 031699031 TOX120, 181T4i6, 2/22/99, dl000 32 031699032 Twl20, ML02229-250ppb-CC4, 2/22/99 33 031699033 34 031699034 Me.08 Blank ZOX120, 102F-k 2/22/99, dl000 35 031699035 TOXlZO, 183F-G6, 2/22/99. dlOOO 36 031699036' T0~120. 104F-06. 2/22/99. d l O O 0 37 031699037 T0~120; 1051-G6; 2ii2/99; dl000 38 031699038 ToXl20, 116F-06, 2/22/99, dlOOO 39 031699039 TOX120, RBL02229-25opeb-ccs, 2/22/99 40 031699040 41 031699041 Me.08 Blank ToXl20. 1171-66. 2/22/99. dlOOO 42 031699042 43 031699043 .ToXl20; 119F-G6; Zi22/99; dl000 TOXl20, 1901-66. 2/22/99, dl000 44 031699044 45 031699045 ToXl20, 1911-GSr 2/22/99. dlOOO TOX120, RBL02229-25oppMc6, 2/22/99 46 031699046 47 031699047 I4.W Blank TOX120. 1691435. 2/22/99 4 8 031699040 TOXlZO. 170T-GS; 2/22/99 49 031699049 50 031699050 51 031699051 52 031699052 - _ ToXlPO. .17lT-G& 2/22/99 ?Ox120.* R B L 0 2 2 2 9 - 2 5 m . 2122199 new Blank ZOXlZO. 173Lco5, 2/22/99 53 031699053 54 031699054 55 031699055 ZOx120. 174T-G5, 2/22/99 T o x J 2 0 . 175r-05. 2/22/99 T0%120, MLO2229-25Oppb-CC6, 2/22/99 56 031699056 ' N&U 8157 031699057 T0~120, ~02229-n2mir-i, z/zz/99 SO 031699050 ToX120, RBLO2229-LiTBlk-2, 2/22/99 59 031699059 TOX120, RBLO222S-5-2, 2/22/99 60 031699060 TOXl20, RBLO2229-1Oppti2, 2/22/99 61 031699061 TOX120, MLO2229-25-2, 2/22/99 62 031699062 63 031699063 Tox120, ML02229-50ppti2, 2/22/99 ToxlZO, RBLO2229-10-2, 2/22/99 64 031699064 65 031699065 TOXl20, RBLO2229-25Oppb-2, 2/22/99 TOXl20, RBLO2229-5OOppb-2, 2/22/99 66 031699066 67 031699067 hX120, RBLO2229-75Oppb-2, 2/22/99 Tox120, RBLO2229-1000ppb-2, 2/22/99 BACK TO MAIN 8td CDnO 5.99 12.00 29.90 59.90 120.00 299.00 599.00 091.00 ' 1190.00 YI 8.474 9.067 9.026 9.006 9.014 9.033 9.020 8.969 9.029 9.033 9.036 9.034 0. 977 0.973 5.103 299.00 299.00 299.00 299.00 299.00 9.060 9.114 9.101 9.050 9.070 9.066 9.070 9.060 9.063 0.105 9.066 9.057 9.065 9.060 9.057 9.061 9.051 9.064 9.056 9.051 9.056 9.005 9.062 9.001 '9.075 9.032 9.085 9.040 9.035 299.00 299.00 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1190.00 ' 9.031 0.907 0.914 0.907 0.976 0.975 0.920 #.si# 1.900 9.045 , 0.071 0.971 0.968 0.898 0.967 1.907 0.969 0.910 8 .goo 0.911 0.911 Arm 1749 114 310 378 777 1043 1973 3360 6297 15755 29424 39904 62763 120 253 65 . 96 120 9673 243 165 262 ' 45. 12476 18935 19227 18644 20438 11041 45 19755 16765 16092 10923 13246 11816 155 1_ 1_ 7-23 9219 9380 10457 10389 3257 3363 2407 13194 121 3473 zcn 5396 12660 49 304 329 768 1172 .i 1901 3354 6672 15526 29963 43016 62400 Wposu. 0749.32 114.33 317.11 378.28 i7i.Ji 1043.01 1973.20 3368. 00 6297.05 15754.60 29423.97 39983.57 62762.04 128.18 253.39 b a a . nwa k0.V 172.09 bb 0.00 bb bb 0.00 bb 0.00 5.39 lQ4 10.94 bb 30.39 bb 59.56 bb 120.61 bb 310.57 bb -10 -9 2 -1 ' 1 7 604.41 W4 1 025.22 bb -0 1301.55 bbX 9 0.00 W4 0.00 bb 64.63 95.67 121.30 9673.44 242.96 164.97 262.30 44.62 12476.30 28.42 11134.95 18935.20 19226.52 18644.16 20437.57 11040.02 45.17 19754.86 16'164.61 16091.88 10923.35 13245.65 11815.70 155.06 11727.19 9219.46 9388.04 10457.05 10389'.20 0.00 'bb 0.00 bb 0.00 bb 191.41 W4 0.00 bb . -36 0.00 bb 0.00 bb 0.00 bb 250.02 bb -16 * 0.00 bb 360.35 lQ4 305.08 bb 391.17 bb 379.00 lQ4 416.50 lQ4 220.00 In4 0.00 bb 402.22 W4 339.69 m 325.63 bb . --221 ) 217.55 W4 . 266.11 bb 236.21 bb 0.00 234.36 b&b 101.91 W4 185.44 IU 207.00 IU 206.31 lQ4 -31 3257.20 3362.65 2407.33 13093.83 120.93 3473.23 2677.29 5396.21 12660.21 49.26 303.77 320.82 767.69 1172.49 1980.14 3353.64 156562726.1096 29962 -79 43815.81 62399.96 57.24 bb 59.45 bb .,* 39.47 bb 2 279.66 bb - 0.00 bb 61.76 bb 45.12 bb 101.97 bb 253.07 bb 0.00 bb . 0.00 bb 0.00 bb 5.19 m 13.65 bb 30.71 bb 59.26 bb 120.65 bb 313.79 bb 615.60 bb 905.35 bb 1293.96 bbX . -6 -15 -13 14 3 -1 7 5 3 1 8 .. Amelia 062490 qu.nut.td ud p r i n t d on 03/17/99 by W --%A- 1 1 1 hintd: -2: Id mr 11 14:33150 1999 .cuz 1 #y.u 1031699001 2 031699002 3 031699003 4 031699004 trplolirt T u t M a o n Blank M a l l Blank ToxlZO, llsLo2229-HZOglk-2, 2/22/99 Toxl20. RBLO2229-LlvBlk-2. 2/22/99 5 031699005 Tox120; ML02229--2, 2/22/99 1 6 031699006 7 031699007 8 031699008 9 031699009 10 031699010 ToxlZO, RBLO2229-10ppb-2, 2/22/99 Toxl20. RBLO2229-25mb-2. 2/22/99 ToxIZO; ML02229-5-2; 2/22/99 Tox120, RBLO2229-looppb-2, 2/22/99 ToxlZO, RBLO2229-2SOppb-2, 2/22/99 11 031699011 Toxl20,' ML02229-50-2, 2/22/99. j .- 12 031699012 13 031699013 14 031699014 15 031699015 Tox120, W2229-75Oppb-2, 2/22/99 Tox120. man iRBaL02229-1000wb-2.. 2/22/99 Tox120, l 6 l W , 2/22/99. d250. 16 031699016 T0x120, 1 6 2 W , 2/22/99,*d250 -1 17 031699017 18 031699018 19 031699019 20 031699020 21 031699021 Toxl20, 163?4, 2/22/99, d250 Toxl20, 164T-65, 2/22/99, d2SO Tox120. 1 6 5 W . 2/22/99. 6250 TOx120; ML02229-zsoppb-cQ, 2/22199 UeOR Blank 22 031699022 tox120. 166F.w. 2/22/99. d250 23 031699023 24 031699024 _ _ 25 031699025 26 031699026 27 031699027 1 28 031699028 Tox120, 167T-65; i/22/99, d250 20x120, 1681-65, 2/22/99. d250 Tox120. ML02229-2SO~~?b-a32./22/99 ~~ ~ w.08 simk p loxl20, 1173166, 2/22/99, dl000 Toxl20, 1783F-GS. 2/22/99, dlOOO 29 031699029 T0X120, 1791-06, 2/22/99, dlOO0 30 031699030 T0x120. 1801166. 2/22/99. dl000 31 031699031 i 32 031699032 /99 33 031699033 I.QH Blank ii 03is99oSi ToXl20, 182P-G6, 2/22/99# dl000 35 031699035 Toxl20, 1811166, 2/22/99, dl000 36 031699036 20x120, 184T-GS. 2/22/99# dl000 37 031699037 ToXl20, 185T-06, 2/22/99, dlOOO 38 031699038 39 031699039 i 40 031699040 I 41 031699041 42 031699042 T0xlZ01 186T166, 2/22/99, dl000 -120, RBU12229-25~CC5, 2/22/99 M.QH Blank Toxl20, 1871166, 2/22/99, dl000 ToxlPO, 189T166, 2/22/99, dl000 43 031699043 ToxlZO, 19OF-G6, 2/22/99, dlOOO I 44 031699044 45 031699045 4 6 031699046 47 031699047 48 031699048 Toxl20, 1911166, 2/22/99, d l O O O TOX120, RBLO2229-25Oypb-CC6~2/22/99 I4an BluIk Tal20. 1691CG5, 2/22/99 ?0%120. 1703-65, 2/22/99 49 031699049 T~120.~1711CG5.2122199 I 50 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 ToX120,' MLo22zkzsopp&ccs, Blunk ToxlZO. 1131-GS. 2/22/99 T0x120. 1141-G5, 2/22/99 roxlf0. 175r-GI. I / Z t / S V ToX120, RBL02229-2SOppbCc6, 2/22/99 2/22/99 3 56 031699056 * 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 W a O l Blank Taxl20, RBLO2229-HZOBlk-2, 2/22/99 TOXl20, RBLO2229-LivBlk-2, 2/22/99 T0xl20, PBLO2229-5ppb-2, 2/22/99 Toxl20, RBL02229-1oppb2, 2/22/99' Toxl20, RBLO2229-ZSppb-2, 2/22/99 ToX120, RBLO2229-5oppti2, 2/22/99 1 63 031699063 64 031699064 6S 031699065 66 031699066 67 031699061 T0~l20,RBLO2229-1OOppb-2, 2/22/99 TOXl20, PB442229-25Oppb-2, 2/22/99 ToX120, PBLO2229-50-2, 2/22/99 TOX120, RBLO2229-15oppti2, 2/22/99 TOXl20, PBLO2229-100Oppb-2, 2/22/99 I I 1 .. i kn+la 062498 q ~ ~ t i + . tandd printad en 03/l7/99 by UC ..---.A BACK TO MAIN 8.412 1.00 0.404 1.00 8.420 1.00 8.404 1.00 8.354 1.00 8.413 1.00 8. 354 1.00 8.358 1.00 8.354 1.00 8.361 8.356 ku 684 2274 2907 2500 2189 . 1931 2004 1623 1549 1175 48 kap0n.m 684.42 2274 -11 2906.68 2499.85 2189.21 1931.02 2004.23 1622.84 1548.68 1774.91 47. 58 82 9 0 1.00 8.370 8.453 1.00 8.379 8.384 8.384, 0.123 1.00 8.380 7.984 1.00 8.446 12 131 1 1174 9 10 14 982 9 955 12.14 736.68 7.06 1113.91 8.97 9.59 14.36 . 981.80 9.15 955.41 0.41 bb -59 1.37 bb 31 1.75 bb 75 __ 1.51 bb 51 1.32 bb 32 1.16 bb 16 1.21 bb 21 0:98 bb -2 0.93 bb -7 1.07 bb 1 0.03 bb -97 0.01 bb 0.0404 bb -99 --15060 0.11 bb 0.01 bb 0.01 bb 0.01 bb 0.59 bb 0.01 bb -29 ' -99 -99 -99 . -41 -99 0.58 bb . -42 1.00 1.00 1.00 1-00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 8.355 8.418 8.228 8.235 8.294 8.361 8.303 a.301 8.230 0.359 8.359 8.290 8.285 8.289 8.289 8.357 8;295 8.286 8.304 8.292 741 1213 601 a7 809 1236 706 658 623 1229 1222 1139 1561 1311 1190 1218 1211 1102 1179 1136 140.87 1213.32 600.53 616.93 809.04 123S.83 106.35 SS57.572 623.03 1229.39 1221.94 1138.70 1561.05 1317.43 1189.57 1277.63 1211.23 1102.30 1178.81 1135.66 0.45 bb 0.73 bb . 0.16 bb +. 0.37 bb ..?: i 0.49 bb 0.74 bb 0.43 bb . 0.40 bb 0.38 bb 0.74 bb 0.74 bb 0.69 bb 0.94 bb 0.79 bb 0.72 bb 0.71 bb 0.73 bb 0.66 bb 0.71 bb 0.68 bb -55 -27 *-64 : -63 -51 -26 -51 -60 -62 -26 -26 -31 -6 -21 -20 -23 -21 -34 -29 -32 . ~ 1 1 - PlUnufY c.libt.uoa -0 Rat L l v u (iroup 5 and : mibraurn: o : b t 8 l . w \ ~ \ o I l c ) o b u s t .odiriid: m i d #r 17 i4:m:io 199s win-: Id I(.t 17 14:34:29 1999 ompound 1 n a 1 oefficlent of Determlnatlon: 0.995680 . allbration curve:47.8224 'x + 519.658 * . esponse type: External Std, Area urve type: Linear, Origin: Exclude,Weighdng: lkAxis trans: None I 6.28e4 1 3 .. .. ,. '3 . . .. . . *. 1 .. I I I I 1 iesponse J '1 I I I 1 .fI 5 t I"" ""1"" '"'I"'' ""1'"'""'"'' 20b.0 40b.O 60b.O 80b.0 t 4 BACK TO MAIN -1 d .3k -: . ' lObO.0 , Conc I 1 1 .. . cuultify Q L i h n t i O a h p o r t m x l 2 0 mt Ltvu 0rOup I ud 6 calibration: O:bt.).?lW\~\031C9Sb L u t mrUfidr kd N u 17 14r37:lO 1999 Rlntd: M w 17 i4:34~as 199s BACK TO MAIN ompound 2 name: SUIT esponse Factor: 1659.45 1 esponse External Std, Area urve type% I 2.91 e3 X 1 3 X '1 . ._.. ." . X X 1 X X 1 .. i X I 1 1 iX X 1 1 I I i i / I 0.1 Cont 012 0!3 014 O!S 0!6 O!? 0!8 0:s l!O I 1 I 1 1 . - nethod Report nethod til.: Last .edified: 0:\rrt8Z.PRO\lhthm\031699b.rrB W e d Mu 17 0S:lbrZO 1999 1 Pms t-I TIC I Cmc. A 1.000000, Di8sbl.d 1.000000 1.000 I 3 I. 1 I i I I 1 _1 Rslrtiw hdghti Absolutm bight.: 3 Rehtiva eru: A b s O l U t S -1 3 I I 1 I I 8.04S, mabled 0.000, Disabld 0.717. mst 193 . Diubled 3 a 30.00 70.00 4.00 . 10.00 DiSabld 10.00 ' 50.00, pI.bld 0.00. D i u b l d 3.00. mubled 0.00, Disabled .. BACK TO MAIN Page : .. . . . .i .n . 3 , .*\ .. surr t-I TIC rhd 1.000000, Disabled 1.000000 2.000 8.231. publad 0.000, D b a b l d , 0.588 PolyZumial Typo: Point of origin: ?it tfeightinut Aria huuformmrionr n ._ . Dis8bl.d 3 a Drad articml throaholdr Detect mhoulduar Shouldor thruholdr 30.00 70.00 4-00 10.00 DI#.hl.d 10.00 50.00. mubL.d 0.00, DZ.&ld 3.00, Bvbld 0.00, Diaab1.d BACK TO MAIN .. 1 1 1 1 I 3 1 1 I I 1 I 1 1 4 1 1 I mantifY ccqound E-Y -Port -20 Rat Livu orwp 5 and 6 .1- Watt h a t wdifid: lCtbod: Irat rodCfid: Job w: Erin-: ' O:\~tI2.~\8~~\031699b I d Ilu 11 13:54:10 1999 a:\rat12 . ~ W . U ~ O B \ O ~ ~ ~ S S ~ Z Id Xu 11 O6:59:09 1999 Id #r 11 U:Ol:l3 IS99 (tun 1031699001 2 031699002 3 031699003 w . U a t 2..t MaOH Blank Mam Blank TOxl20, RBLO2229-U2mlk-2, 2/22/99 4 031699004 Toxl20, RBLO2229-Lialk-2, 2/22/99 5 031699005 Toxl20, RBLO2229-5-2, 2/22/99 6 031699006 T0~120,RBLO2229-10ppb2, 2/22/99 7 031699007 Toxl20, RBL02229-25ppb2, 2/22/99 S 031699000 ToXl20, ML02229-50ppb2, 2/22/99 9 031699009 T0xl20, RBLO2229-100ppb-2, 2/22/99 ' 10 031699010 T0x120, RBLO2229-25oppb-2, 2/22/99 11 031699011 Tox12O,~RBLO2229-5OOppb-2, 2/22/99 12 031699012 T0xl20p RBL02229-75-2, 2/22/99 13 031699013 Toxl20, MLO2229-1OOOppb-2, 2/22/99 14 031699014 xO. H Blank 15 031699015 Toxl20, 1 6 1 W , 2/22/99. d250 16 031699016 TOXl20, 1 6 2 W . 2/22/99, .a50 11 031699011 TOx120, 16334, 2/22/99. d250 1s 031699010 Toxl20, 164?-C5, 2/22/99. d250 19 031699019 Tqx120, 165?+5, 2/22/99, d250 ' 20 031699020 Toxl20, RBLO2229-25opgMc2, 2/22/99 21 031699021 h r n Blank 22 031699022 Toxl20~-166?-05, 2/22/99, d250 ' 23 031699023 ToXl20, 16l3-65, 2/22/99. d250 24 031699024 Tox120, 160T4, 2/22/99, e 5 0 25 031699025 ToXl20, RBLO2229-25Oppb-COp 2/22/99 ~~ 26 P31699026 w.oH Blank 27 031699027 TOxl20, 177k6, 2/22/99, dl000 20 031699020 ToXl20, 178v-G6, 2/22/99, d l O O 0 29 031699029 ToXl20. 119?-G6. 2/22/99. dl000 30 031699030 ~0x120;ieor-ts; njujos; a 0 0 0 31 031699031 ToXl20, lSlI-CC, 2/22/99, dl000 32 031699032 ToXl20, RBL02229-25Oppb-CC4, 2/22/99 33 031699033 MmOH Blank 34 031699034 ToXl20, 182Pk6, 2/22/99. k 0 0 0 35 031699035 ToX120, 183F-GS. 2/22/99. dl000 36 031699036 Toxl20, 184T-C6, 2/22/99, til000 37 031699037 38 031699038 39 031699039 40 031699040 _ _ TOxl20, 1853-G6, 2/22/99, dl000 ~0x120,iim-~s,2/~2/99.a 0 0 0 ToX120~RBL02229-250arb-CW. 2/22/99 H w t Biank 41 031699041 T0xl20, 187i'-G6, 2/22/99, dl000 42 031699042 90x120, 1893-CS. 2/22/99, dl000 43 031699043 Tox120, 1903-G6, 2/22/99. dl000 44 031699044 ToxlZO, 191M6, 2/22/99, dl000 45 031699045 ~0x120,~~0~229-2soppb-a26/,22/99 46 031699046 NaOH Blank 47 031699041 Tox120. 169F-GS. 2/22/99 48 031699048 Tox120. 170F-05, 2/22/99 49 031699049 Toxl20. . 1 7 1 W , 2/22/99 SO 031699050 Toxl20,. RBL02229-25oppbCC5, 2/22/99 51 031699051 X w t BlUlk 52 031699052 ToX120. 1733-65, 2/22/99 53 031699053 54 0316990S4 55 031699055 Toxl20. 1 7 4 W , 2/22/99 Torl20. 175?-t?S, 2/22/99 ~0x120,~ ~ ~ 0 2 ~ ~ 9 - 2 ~ ~2p/2p2/b99. a ~ . 56 0316990S6~ H a Blank 57 031699051 Toxl20, MLO2229-li2ak-2, 2/22/99 50 031699050 ToX120, RBL02229-Ll~Blk-2,2/22/99 19'031699059 T0xl20, RBLO2229--2. 2/22/99 60 031699060 Toxl20, -02229-1Oppb-2, 2l22/99 61 031699061 Toxl20, RBL02229-25ppb2, 2/22/99 62 031699062 TOXl20, MLO2229-5-2, 2/22/99 63 031699063 Toxl20, MLO2229-10-2, 2/22/99 . 64 031699064 TOxl20, MLO2229-25oppbZe 2/22/99 65 031699065 T0xl20~RBC02229-50oppti2, 2/22/99 66 031699066 Toxl20, MLO2229-15-2, 2/22/99 61 031699061 TOx120. RBL02229-1000ppb-2e2/22/99 1 i i J * : BACK TO MAIN 5.99 12.00 29.90 5S.90 120.00 299.00 599.00 S90.00 1108.00 1.870 7.029 1.012 7.020 1.020 1.826 1.B30 1.026 1.832 1. (30 "1.893 ' 1.049 1. 901 . 7.0E9 299.00 1.850 299.00 1.857 1.193 1.057 1.050 52 93 216 32 5 151 . 1966 3677 5126 1909 2001 2569 2200 , 2150 2115 2299 ' 2120 2652 1786 2773 11.01 92.91 216.31 325.31 750.60 1965.81 3616.73 5125.94 1909.42 2001.43 . 2569.44 2199.04 2149.83 2114.90 2299.14 5.06 bb 12.91 M 34.09 iQ4 52.81 bb '125.S3 bb 334.50 bb 620.26 bb 877.10 bb 1355.04 bbX 341.64 IM 430.13 IM 374.67 #I 366.09 Lw '463.11 m 391.12 bb -2 0 14 -12 5 12 5 -2 13 .. 2120.11 2651.91 1105.69 2712.08 452.31 iQ4 303.56 iQ4 473.01 IW 50 . 299.00 1.049 1900 1979.51 336.05 bb . 13 299.00 1.851 2105 2104.76 372.08 bb. 24 299.00 299.00 299.00 1.821 1.089 1.166 1.770 1.102 1.031 1.115 7.711 1.76S 2065 2303 999' 1350 1319 234 175 203 1290 5.99 12.00 2~9..90 59.90 120.00 299.00 599.00 098.00 1190.00 1.161 1.157 1.162 1.163 1.766 1.170 1.159 1.175 1.166 46 99 ..'\ _170 328 130 1110 3403 5157 1163 2064.91 351.50 &W 2303.09 998.60 1350.47 1319.16 233.79 175.09 202.65 1290.25 392.40 bb .160.41 bb . 220.03 iQ4 .:.2.3, 223.45 bb 27.01 IPI ' 31.74 bb 219.86 bb 10 . )& -25 -26 46.3s 90.73 171.91 . 320.19 130.44 1709.98 3402.n 5157.15 1163.23 4.91 l4l -10 13.90 bb 16 21.51 #I -0 53.30 141 -11 122.31 M 2 290.56 l4l -3 581.22 141 -3 082.46 bQf . -2 1226.91 bbX 2 1 1 1 I 1 7 '1 1 1 ! I 1 1 .'j 3 purnurv CosOMd .rauy TOXlZO mat L i v u Oroup 5 and 6 a&. I t a t : o:\a+.2.rw\h.pr-\o3i6g@b k m t mdlfid: K d #I 17 13:Sl:lO 1999 H.Ulodr Q:\r~+12.~~thOB\031699bZ k m t d f i d :Id W 17 06:59:09 1999 Job Cod.: Riatdr K d W 17 L5:ol:U 1999 colgound 2: 0w.r i 031699001 2 031699002 3 031699003 4 031699004 I031699005 6 031699006 7 031699007 8 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 15 031699015 16 031699016 17 031699017 10 031699018 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 o3irosor.r 28 031699028 29 031699029 30 031699030 31 031699031 32 031699032 33 031699033 34 031699034 35 031699035 36 031699036 37 031699037 38 031699038 39 031699039 40 031699040 41 031699041 42 031699042 43 031699043 44 031699044 45 031699045 46 031699046 47 031699047 48 031699048 49 031699049 50 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056. 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067 W&H B l.& Toxl20, PBLO2229-IUmlk-2, 2/22/99 ToY120r -2229-LbBlk-2, 2/22/99 Tox120, m2229-5ppti2, 2/22/99 Tox120, RBL02229-1oppti2, 2/22/99 Toxl20, RBLO2229-25-2, 2/22/99 ~- -. Toxl20, RBLO2229-5Oppb-2, 2/22/99 Tar120. RUI.0_ 222_ 9-1O_O.aub-2. e_- 2~-/22/99 ~ Toxl20, MLO2229-25Oppb-2, 2/22/99 Toxl2O,'ML02229-500ppb-2, 2/22/99 20x120, RBL02229-75-2, 2/22/99 Tox120, MLo2229-100oppti2, 2/22/99 X.OB Blank TOXlZO, 161FG5, 2/22/99, d250 Toxl20, 1cZM5, 2/22/99. .&SO * TOXl20, 1631-GS. 2/22/99. d250 Toxl20, 1641-65, 2/22/99, &SO Toxl20, 1 6 5 1 4 . 2/22199. dZS0 Toxl20. llBL02229-25O~~b-a2.2/22/99 MeW B h Toxl20, 1 Tox120, I Toxl20, I TW120, I] l4.m B1.a TOX120, I Toxl20, I i61cGJ, 2/22/99. d250 i73-65, 2/22/99, d250- ;8T-G5, 2/22/99, d250 : t P llo2229-250ppb-cc38 2/22/99 7T-66, 2/22/99, dl000 '81T46, 2/22/99, d l O O 0 Toxl20, 1 ' 9 M 6 . 2/22/99. dl000 T0x120, 1 IOF-bb; i k i i 9 9 ; dl000 Tox120. I Il?-G6, 2/22/99, dl000 1102229-25Oppb-CC4, 2/22/99 h1-k 2/22/99. dl000 Toxl20; 183146; 2/22/99; dl000 Tox120, 104146, 2/22/99, dl000 Toxl20, 105M6, 2/22/99, dl000 _ _ ToX120, 106146, 2/22/99, Tox120. RBLO2229-25-. W ~ nHiu* Tox120, 107146. 2/22/99, dlOOO 2/22/99 dl000 Iroxl20, 189T46, 2/22/99, dl000 Tox120, 190M6, 2/22/99, dlOOO Toxl20, 191T-G6, 2/22/99, dl000 ToXl20, W02229-25Oppb-CC6, 2/22/99 Warn Blank Tox120. 1691cGS. 2/22/99 Tox120. 170T-G5, 2/22/99 Toxl20. 171T45, 2/22/99 Tox120, R B L O 2 2 2 9 - 2 5 ~ ,2/22/99 MmOU B h n R Toxl20. 173T-GS. 2/22/99 Tox120. 174T-G5, 2/22/99 'Mxl20. 1?5WS, 2/22/99 Toxl20, RBLO2229-250ppb-CC6, 2/22/99 MeOH Blank Toxl20, R8LO2229-IIZalk-2, 2/22/99 T0~120, W.02229-LivBlk-2, 2/22/99 Tox120, RBLO2229--2, 2/22/99 Toxl2O8 RBL02229-1oppti2, 2/22/99 ZOllPO, MLo2229-2-2, 2/22/99 20x120, mLO2229-5oppb-2, 2/22/99 Tox120, BdLO2229-10-2, 2/22/99 Tox120, MLO2229-25Oppb-2, 2/22/99 T0~l20, RBIO2229-50Oppb-2r 2/22/99 Tox120, llBLO2229-750ppb-2, 2/22/99 ToxlZO, MLO2229-100Qppb-2, 2/22/99 BACK TO MAIN 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 0.412 0.482 . 0.404 8.420 8.404 0.354 8.413 8.354 0.35. , 8.354 8.361 8.356 684 904 2274 2907 2500 2109 1931 ZOO4 1623 1549 1775 40 684 .42 903.69 2274.17 2906.68 2499.85 2189.27 1931.02 . 2004.23 1622.84 1548.68 1774.97 47.58 0.41 bb -59 0.54 bb -46 1-31 bb 37 1.75 bb 75 1.51 bb 51 1-12 bb 32 1.16 bb 16 1.21 bb 21 0.98 bb -2 0.93 bb -7 1.07 bb 7 0.03 bb -97 8.290 12 1.00 0.378 737 8.453 7 . .1.00 8.379 8.384 8.38! 8.123 1.00 8.380 Analy WY .. Qc. -1Y 2:; -1Y . Wly. gc WY WlY Woc l Y . m1y -Y WlY -o1c.y -1Y -1Y aa1y Stud ' ptud stud stand stud stud Stand StUd stand . 7.984 1.00 0.446 1.00 0.355 . 0.418 8.228 8235 1.00 0.294 .1.00 0.361 8.303 8.301 0.230 1.00 1.00 1.00 '1.00 1.00 1.00 1.00 1.00 1.00 0.359 8.350 8.290 8.285 8.219 8.209 8.357 0.295 0.286 8.304 0.292 1174 9 10 14 982 955 741 1213 601 617 809 1_ 2_36~ ~ 706 658 67.3 1229 1222 1139 isn .*\ 1317 1190 iz78 1211 1102 1179 1U6 12.14 ,736.68 7.06 ' 1173.91 8.97 9.59 14.36 Sei. eo 9.15 955.41 0.01 bb 0.44 bb 0.00 bb. . -99 -56 -100 0.71 bb 0.01 bb 0.01 bb . -29 -99 -99 0.01 bb -99 0.59 bb . -41 0.01 bb -99 0.58 bb. -42 740.07 0.45 bb 1213.32 600.53 616.93 809.04 1235.83 706.35 - 657.12 623.03 . .$ '? . 0.73 bb 0.36 bb 0.37 bb 0.49 bb 0.74 bb 0.43 bb 0.40 bb 0.10 bb 1229.39 1221.94 1130.70 1567.05 . 1311.43 1189.57 1277.63 1211012.2330 1170.81 1135.66 0.74 bb 0.74 bb bb 0.69 bb 0.94 bb 0.19 0.72 bb 0.77 bb 0.73 bb 0.66 bb 0.71 bb 0.68 bb . -5s -21 * -64. -63 * -51 -26 -57 -60 -62 -26 -26 -31 -6 -21 -28 -23 -27 -34 -29 -32 .. 1 I :ompound 1 name: PFHS :oefficientof Determination: 0.997758 . 1 :alibrationcum: 5.82393 x + 17.7720 . lesponse type: External Std, Area :uwetype: Linear, Origin: Exclude,Weighting: l/x, Axls trans: None 1 7.9183 1 f 4 7' ... I i f I 1 1 -1 I I 1 1 I I BACK TO MAIN 181 :ompound 2 name: Sum qesponse Factor: 1659.45 qesponse ty e External Std, Area >UNe type: {F 2.91 e: Response BACK TO MAIN X X . . . ...._;.... .. .. . . . . X X X X X 0 Conc 1 Otl Of2 0!3 014 0!5 O!S 0!7 O!S 0!9 l!O 1 n8tbd mort PUL Wactionr . NeWt BACK TO MAIN _ .c .. *_ . .. .. . , 50.00; lhubld 0.00. D i s . b l d 3.00, -1.d 0.00. D i s a b l d 1 . t I f ".i i . xethod Report f LzSEr: prunt$fy hac.: conc. o f 8t.nd.td.X u s e RP valu.: U a u Pa& Pactor: mt.gr.t. w ~ d a ~r .ctor: 1.000000, 0L.bl.d 1.000000 1.000 1' U i t 8 : 1- P.&-to-p..k 1- uplitud.1 71 Diubld ,3 a .. 30.00 70.00 4.00 10.00 Diubld : 10.00 , 50.00. 0.00. mlabld Diubld 3.00, P+.bld . 0.00. Diubld I BACK TO MAIN Pas. ... : P ! i . BACK TO MAIN I"""""" .. 1 I 8 I i 1 I 1 3 1 I I I i I 1 I 3 ! 1 I 1 .f BACK TO MAIN Qruntify Colnpound S-Y ?OX-120 Rat Liver hport -* &kat: N : \ ~ ~ 2 . ~ \ S ~ 1 9 B \ 0 4 2 0 9 9 a m a t Uadifid: mu Apr 22 09:02:11 1999 H.thcd: N:\Xat82.~~thOB\042099a b a t modifid: Tu- M y 04 08:52:41 Jobcod.: . Printd: W d Hay 05 11:10:51 1999 1999 8 Nu suaplaliat h.t - 8td COM a? 1 042099002 H8OH Blank 2 042099003 ToXl20, RBLO4199-H2OBlk-1, 4/19/99 31 042099004 Twl20, RBL04199-LivBlk-1, 4/19/99 4.483 4 042099005 I, 042099006 6 042099007 Toxl20. RBL0419+5~~b-1.4/19/99 TOXl20, RBLO4199-lbppb-1, 4/19/99 ToXl20, RBLO4199-25ppb-1, 4/19/99 standard Standard Standard . 5.56 11.10 27.80 4.480 4.477 7 042099008 Toxl20, RBLO4199-SOppb-1, 4/19/99 Standard 55.60 4.479 8 042099009 Toxl20, RBLO4199-1OOppb-1, 4/19/99 Standard 111.00 4.479 9I 042099010 Toxl20, RBL04199-250ppb-1, 4/19199 standard 278.00 4.486 10I 042099011 ToXl20, RBL04199-500ppb-1, 4/19/99 Standard 556.00- 4.484 11 042099012 TOX120, RBLO4199-75Oppb-1, 4/19/99 standard. 833.00 4.481 12 042099013 ToXl20, RBLO4199-100opeb-1, 4/19/99 Standard 1111.00 4.484 13 042099014 Blank 1154, 042099015 042099016 TOXl20, RTL, UI, Gl, 4/19/99 Toxl20, RTL, W , 01, 4/19/99 16I 042099017 TOX120, RTL, 34, Gl, 4/19/59 17 042099018 TOXl20, RTL, 4U. GI, 4/19/99 1 8 04209901P Tox120, 1%. W, 01. 4/19/99 19I 042099020 20I 042099021 TOx120, RBL04199-25QPpb-CCl. 4/19/99 Qc WeOH Bhnk 278.00 4.483 21 042099022 ?OX120. R T L W. 01, 4/19/99 21' 042099023 TOX120, RTL, fx, Gl, 4/19/99 23I 042099024 ToW120, RTL, 8w, el, 4/19/99 24 042099025 TOX120, RTL, MI G I r 4/19/99 25 042099026 26, 042099027 27 042099028 a 20x120, RTL, 10th Gl, 4/19/99 ToX120, RBL04199-250ppticQ, 4/19/99 MeOH Blank 278.00 4.480 28 042099029 TOX120, RTL, 1111, Cl, 4/19/99 29I 042099030 TOX120, RTL, l3M. Gl, 4/19/99 30I 042099031 ToxlZO. RTL. la. G1. 4/19/99 31 042099032 32 042099033 33 042099031 . 278.00 4.484 34 042099035 TOXlZO~-RTL,1'111. 62, 4/19/99 4.480 35 042099036 TOXl20, RTL, lBX, 62, 4/19/99 4.482 36 042099037 TOXl20. RTL, 1M. 62, 4/19/99 4.480 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 TOX120, RTL, 2W. G2, 4/19/99 oc Tox120. RTL. 211. 62. 4/19/99 TOY120; RBL0419912SQpb-CC4 0 4/ 19/99 H8OH Blank ~0x120.RTL. zm. 62. 4/19/99 - 218.00 4.411 4.485 4.476 4.479 42 042099043 TOX120; RTL; 23M; C2; 4/19/99 4.479 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048 ToX120, RTL, 24X, 62. 4/19/99 Toxl20. RTL. 2%. 62. 4/19/99 _ _ oc Toxl20; RTt; 26n; G2; 1/19/99 Tox120, RBLO4199-25O~pb-CC5,4/19/99 WeOH Blank . 4.487 4.485 4.484 278.00 4.479 i 8 042099049 ToxlZO, RTL, 2 m , 62, 4/19/99 4.484 49 042099050 Toxl20, RTL, 2%, 62, 4/19/99 4.483 50 042099051 Tbrtl20, RTL, 301(, G2, 4/19/99 ' 4.479 51 042099052 52 042099053 53 042099054 54 042099055 55 042099056 56 042099057 57 042099058 a rox120, RTL, 3111, 67, 4/19/99 Toxl20, RBLO4199-2SOppb-CC6, 4/19/99 W80H 81ank Tox120, nn., a ~ a. . 4ii9199 ToXl20i RTL, 34% 63. 4/19/99 Tox120, RTL. 3%. G3. 4/19/99 TOX120, RTLi 3QI; '03; 4/19/99 278.00 4.479 4.483 4.481 4.479 4.479 4.495 58 042099059 TOXl20, RTL, 37M, 63. 4/19/99 4.453 59 042099060 60 042099061 TOX120. RBL04199-250~8b-CC7, 4/19/99 Qc 278.00 4.495 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 66 042099067 67 042099068 Toxl20, RiL, 3W G3, 4/19/99 ~0x120, nn, 3%. 63. 4/19/99 ~0x120,nn, 4011. W, 4/19/99 ~0x120,nn, din. W, 4/19/99 ToxlZO; RTL, 42M. 63, 4/19/99 Toxl20, RBL04199-.25Oppb-CC8, 4/19/99 Qc W8OH Blank 278.00 4.472 ,4.463 4.475 4.488 4.481 4.480 . 68 042099069 69 042099070 70 042099071 71 042099072 '72 042099013 73 042099074 74 042099075 Toxl20, RTL, 4%. G3. 4/19/99 Tox120, RTL, 4% G3. 4/19/99 Tox120, RTL, 4% G3, 4/19/99 Toxl20, RTL, 4tw. G3. 4/19/99 Tox120, RBL04199--250ppb-CCS. 4/19/99 gE neon Blank Tox120. RTL. 6% G5. 4/19/99 4.483 4.419 4.480 4.406 278.00 4.484 . 75 042099016 76 042099077 Toxl20; RTL; 66H; GS. 4/19/99 Tox120, RTL, 7% G5. 4/19/99 ' 71 042099018 ToxlPO. RTL. 74M. G5, 4/19/99 78 042099079 Toxl20; RTL; 7%; G5r 4/19/99 79 042099080 Toxl20, RBL04199-*25Op~b-CC44~/19/99 Qc 80 042099081 l4.W Bimk a i O ~ Z O ~ ~ O O Z TOX120, RTL, 7'111, G5. 4/19/99 278.00 4.454 82 042099083 TOXl20, RTL, 7 W , G5, 4/19/99 83 042099081 Tox120, RTL, 7%. 65, 4/19/99 14 04209900s Toxl20, RBLO4199-25Oppb-CC5, 4/19/99 Qc 278.00 4.459 85 042099006 NOOH 81Jnk 86 042099087 TOXlZO, RTC, '34, Gl-Z50ppb-M, 4/19/99 4.477 87 042099008 Toxl20. RTL, 34, G1-25Oppb-MSD, 4/19/99 4.472 88 042099089 TOXl20, RTL, 6H. Gl-ZIOppb-blS. 4/19/99 4.485 D.9. 1 Ani 74 57 77 121 165 374 740 922' 1075 -. 73.837 57.350 76.531 121.098 165.361 374.157 739.533. 921.621 1074.879 COW. r h g a u)av 32.87 )P( 20.70 M 34.85 ml 67.75 )P( 100.42 bb 254.53 bb 524.21 k H 658.60 bbX 771.72 bbX . 86 25 22 -10 -8 -6 -21 -31 355 354.908 240.32 bb 441 447.316 308.53 bb 11 341 341.490 230.42 IQI -17 3899 5143 5371 5320 5764 413 4423 3922 5424 2795 3068 343 3898.614 5142.572 5371.353 5319.818 5764.340 413.054 2855.93 bb 3774.03 bb 3942.89 bb 3904.90 bb 4232.95 bb 283.24 bb 4422.820 3922.056 5424.250 2194.S36 3067.762 342.693 3242.19 bb 2873.19 bb 3981.94 bb 2041.05 bb 2242.64 bb 231.30 #I 1-17 2119 2119.342 1542.63 bb 1448 1448.030 1047.14 bb 1545 1544.621 1118.43 bb . 1644 1644.361 1192.05 bb 419 418.663 287.38 hb 3 . 4232 4232.225 3102.12 bb 5668 5666.119 4161.93 bb 4583 4 657 4582.970 3361.00 bb 4657.182 3415.77 m 5081 ' 5081.245 3728.77 bb 411 411.447. 282.0s "I 56q 4587 5604 3410 1319 443 5631.017 4134.55 m 45B7.391 3364.27 m ~ 6 0 3 . ~ 94114.33 bb 3409.BOS itss.10 m 1318.606 951.61 bb 442.641 305.07 bb 2595 1076 8 50 1384 , 390 2594.520 1893.35 bb 1075.927 772.50 bb . 850.322 605.98 bb 1383.690 999.65 bb 391.147 272.20 M, -2 413 364 363,646 246.77 lQ4 -11 372 312.063 252.98 bb 380 310.105 258.92 tW 385 384.795 262.38 bb 14; w O! b4f ofI i f BACK TO MAIN 1 1 9umtW C=wUnd 8 - y TOX-120 R a t I A W k.port 0-1. L h t l w:\~t~2.AIO\O.npl.Da\OllOOlo Lwt modifid: h u Apr 22 09:02:ll i9ss H.thod: R: \rat.2.OaO\W.thDB\042099a L a a t wdifid: Tu. U8y 04 01:12:41 1999 I Job cod.: Printd: W d May 05 11:10:51 1999 ccqmund 1: WM - .. ---- : Pam 2 Iy..u 09 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 042099097 97 042099098 ga 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105 8m.li.t M rrp. ToX120, RTL, a,C1-25Oppb-NSD, 4/19/99 EM- ToX1201 RTL, 1111, Gl-250ppb-NS. 4/19/99 TOXl20, RTL, 1111, G1-25OppMSSDe 4/19/99 Tox120, RBL0419~25oppticc6,4/19/99 OC 278.00 U&H Blank T0xl20, RBLO4199-WOBlk-1, 4/19/99 ToXlZO. RBL04199-LivBlk-1, 4/19/99 TOXl20, RBL04199-5ppb-1, 4/19/99 Standard 5.56 TOXl20, RBLO4199-lOppb-1, 4/1/99 ' TOxl20, RBLO4199-25ppb-1, 4/19/99 Tox1*20, RBL04199-5Oppb-1, 4/19/99 T0~120,RBL04199-100ppb-1, 4/1/99 . Standard Standard Sc.nd.rd Standard 11.10 27.80 15.60 111.00 Toxl20, RBLO4199-25Oppb-1, 4/19/99 Standard 270.00 Toxl20, RBLO4199-500ppb-1, 4/19/99 Standard 556.00 T00x120, RBLO4199-75-1, 4/19/99 IWrd 833.00 ToX120, RBL04199-100Oppb-1,~4/19/99 ..St&d . . .. 1111.00. RT 4.403 4.484 4.486 4.475 4.405 4.419 4.484 4.483 4.483 4.484 4.470 4.405 4.404 4.441 Am 397 492 . 449 377 110 10 57 09 . '. 107 173 301 047 1080 1241 A..ponm 397.081 492.434 449.098 376.897 a n a . Ilaga t D . v 271.45 bb 341.03 bb 309.84 bb 256.55 IM ' -0 110.250 17.053 56.918 88.793 107.191 172.713 300.632 047.164 1000.222 1247.489 59.74 bb 0.00 bW 20.38 bb 43.90 W 57.48 bb 105.04 bb 259.31 bb 603.65 bW 775.67 W 899.12 bbX -100 84 58 3 -5 -7 9 -7 -19 .. . * . 1 t .. f'4 -\Y I . ' u d . l f n . 041099::quanfftatd and p r i n t d PH W O W 9 9 BACK TO MAIN 6-1. List: w:\~t.2.PM\6~l~8\0420990 -st wdifid: fhu Apr zz os:o2:ii is90 mthod: N:\rAt.2.Pao~thDB\O42099a m s t podifid: N m b y 04 08:52:48 1999 Job Cod.: Drintd: W . d May 05 11:10:51 1999 cappound2: PlOI 8 w..u 1 042099002 2 042099003 3 042099004 4 042099005 5 042099006 6 042099007 7 042099008 8 042099009 9 042099010 10 042099011 t l 042099012 I2 042099013 13 042099014 14 042099015 15 042099016 16 042099011 11 042099018 18 042099019 19 042099020 20 042099023 21 042099022 ' 22 042099023 23 042099024 24 042099025 25 042099026 26 042099021 27 042099028 ' - 28 042099029 29 042099030 30 042099031 31 042099032 32 042099033 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 42 042099043 43 042099044 44 042099045 45 042099046 46 042099041 47 042099048 48 042099049 49 042099050 I O 042099051 51 042099052 . 52 042099053 53 042099054 54 042099055 55 042099056 56 042099057 51 042099018 58 042099059 59 042099060 60 042099061 61 042099062 62 042099063 63 042099064 .64 042099065 65 042099066 66 042099061 61 042099068 68 042099069 69 042099010 10 042099071 11 042499012 1 2 042099073 1 3 042099014 74 042099075 75 042099016 16 042099011 17 042099078 78 042099079 79 042099080 80 042099081 81 042099082 e2 04209908~ 83 0420ssoa4 84 042099085 85 042099086 ' 86 042099087 87 042099088 88 042099089 -1aliat T u 2 MeQH Blank Toxl20, RBL04199-WOBlk-1; 4/19/99 TOxl20, RBL04199-LivBlk-1, 4/19/99 Tox120, RBLO4199-5ppb-1, 4/19/99 Toxl20, RBLO4199-10ppb-1, 4/19/99 Tox120, RBLO4199-25ppb-1, 4/19/99 10x120. RBL04199-30ppb-1, 4/19/99' Toxl20, RBL04199-100ppb-1, 4/19/99 10x120. RBLO4199-250ppb-1, 4/19/99 T ~ l Z O ,RBL04199-500ppb-1, 4/19/99 ToxI20, RBLO4199-15oppb-1, 4/19/99 10x120, RBLO4199-1OOOppb-1, 4/19/99 XeOH Blank 10x120, RIL.' M, Gl, 4/19/99 Toxizo, RTL, m, GI, 4 1 w s s 10x120. RTL. 311. G1, 4/19/99 Tox120, RIL. 4Il. G1, 4/19/99 Tox120, RTL. Y(, Gl, 4/19/99 10x120, ~ 0 4 1 9 9 - 2 5 o p p M c 1 , 4/19/99 h o W Blank mi 10x120. RIL. Ql.GI. 4/19/99 Toxl20; RTL; GI; 4 1 1 ~ 9 s TOrl20, RTL, 8& Glr 4/19/99 10x120, RTLI 9n. G1, 4/19/99 _ _ TOXl20,.RTL, 1CU. G1, 4/19/99 10x120. RBLO4199-25ODDb-aZ. 4/19/99 nlax BiAnk 10x120. RTL. 1% G1, 4/19/99 Toxl20. RTL, 13M. GI. 4/19/99 ToXl20; RTL; 1411; G1; 4/19/99 ToxlZO, RTL. 1% GI, 4/19/99 h O H Blank Toxl20, RTL, im, GZ, u w 9 s Toxl20, RTL, 1SX. 62, 4/19/99 Tox120, RTL, 1% G2, 4/19/99 10x120. RTL, 2oDI. G 2 r 4/19/99 Toxl20,. RTL. 2M. 62, 4/19/99 Tox120, RBLO14199-25Oppb-CC4. 41'19/99 n a 11-l: 10x120, RTL. 2234. GZ. 4/19/99 ToX120, RTL. 2.34, 02, 4/19/99 Toxl20. RTL. 2411. G2. 4/19/99 Toxl20; RTL. 2%; G2, 4/19/99 Tox120, RTL. 2611. G2. 4/19/99 . Tox120, RBL04199-250.~_~b-cCS.4I19/ 99 MaoW Blank toxizo, RTL. zm. a,4/is/ss TOxl20, RTL. 2%. G2, 4/19/99 TOXUO, RrL, 3m. GZ, 4/19/99 Tox120, RTL. 3111, G2, 4/19/99 Tox120, RBLO4199-25Oppb-CC6, 4/19/99 M.OH Blank Toxl20, RTL. 3M. G3, 4/19/99 Toxl20,. RTL. 34.3 G3, 4/19/99 T0x120. RTL. 3% G3. 4/19/99 stuldard StAndard StAdArd StAndArd Standard StAndArd 5t~ndArd Standard. StAndArd cc Qc ac Qc- cc ac ac ~ 0 x 1 ~ 0R,n , 3m. 63, 4/19/99 Toxl20, RTL, 3%. G3, 4/19/99 Toxl20, RTL, 4W.. 63, 4/19/99 TOXl20, RTL, 4% 63, 4/19/99 toxizo; RTL. 4m. ~ 3 4, /19/99 Tox120, RBL04199-25Oppb-CC8, 4/19/99 gc MegR Blank TOXI~O, RTL, 4311. 63, 4/is/gs Tox120, RTL. 4% G3, 4/19/99 Tox120, RTL. 4611. 63, 4/19/99 T W O , RTL, 47% 63, 4/19/99 ' TOx120, RBL04199-250ppb-CC91 1/19/99 pc MaOH Blank Tox120. RTL. 6%. 'GI. 4/19/99 Tox120; RTL, 6Q(; G5; 4ii9i99 Toxl20, RTL. 1% 65. 4/19/99 Toxl20, RTL. la, GI, 4/19/99 Tox120, RTL. 7 9 , GS, 4/19/99 . Toxl20. RBLO4199-25o_pp- b-CC4. 4/19/99 UoQH Blank Ioxl20, RTL, l l H , 65, 4/19/99 . Qc Tox120, RTL. 181. G5, 4/19/99 TOXIPOI RTL. TWO G5, 1/19/99 Toxl20, RBL04199-25Oppb-CC5, 4/19/99 Qc NeOH Blank Toxl20, RTLr 34, G1-21Oppb-US, 4/19/99 TOXIZO, RTL, Gl-ZSOppb-MSSD, 4/19/99 Toxl20, RTL, 611, G1-2SOppb-Iu, 4/19/99 5.36 11.10 21.10 SI. 60 111.00 278.00 556.00 833.00 1111.00 4.822 4.823 4.819 4.816 4.819 4.818 4.825 4.823 4.820 4.719 278.00 4.822 4.818 , 4.824 4.811 4.180 4.822 278.00 4.824 4.820 4.823 4.823 4.821 4.820 278.00 4.823 4.824 4.824 4.821 4.824 278.00 4.819 4.821 4.819 4.810 4.779 4.813 218.00 4.819 4.819 4.821 4.824 4.118 4.818 218.00 4.823 4.822 4.818 4.819 4.823 278 .oo 4.820 4.019 4.818 4.181 4.789 4.188 278.00 4.811 4.803 4.813 4.182 4.820 4.819 178.00 4.823 4.818 4.820 4.825 4.823 218.00 4.824 4.819 4.180 4.190 4.804 4.191 278.00 4.810 4.816 4:113 4.195 4.792 4.816 4.811 4.181 114 142 182 310 51 0 835 1912 3926 1290 6382 113.879 142.359 181.845 310.291 510.134 834.915 1972.331 3925.185 3290;446 6381.225 0.00 bb 3.38 bb 8.76 bb 26.24 bb 53.43 bb 97.63 bb 252.40 bb 518.22 BM 103.92 IQo( 852.48 bbX 1409 1061 863 1032 114 2082 1409.125 1066.545 ' 863.333 1032.293 874.013 2081.914 115.16 bb 129.14 bb 101.49 bb 124.48 hb 102.95 WI 261.31 bb IO01 1001.439 121.10 bb 1047 1046. 567 126.43 'bb is42 1341.526 166.56 bb 956 956.335 114.15 bb ' 2596 2595.882 ' 331.25 bb 2251 2256.951 291.13 bb 5 '2384 804 907 013 1926 ' 2383.945 89064.10963 813.112 1926.462 308.41 bb 93.44 bb 107.39 bb 94.61 bb 246.16 bb 514 413 411 383 311 2309 514.195 412.578 411.485 383.146 370.594 2309,180 53.98 bb 40.16 bb 40.01 bb 36.15 W 34.44 bb 298.28 bb 309 259 330 360 301 2006 309.189 259.48 6 330.015 360.292 307.438 2006.424 26.09 bb 19.32 bb 28.92 bb 33.04 bb 25.85 bb 251.04 bb 304 444 810 349 2312 642 401 581 545 669 2265 304.184 25.41 bb 444-249. 44.46 bb 610;409 348.588 . 94.29 b b 2 31.45 bb 3 2312.079 298.63 bb . 642.037 . 11.38 bb 406.537 so.22 bb 586.903 63.88 bb 544..943 58.11 bb 669.401 15.10 bb 2264.938 292.22 bb ' 472 i 113805 421 588 2329 472.107 713.120 804.753 421.200 581.601 2328.665 461 559 309 330 2293 486.621 538.18k 109.226 529.102 2293.406 48.26 bb 81.05 bb 93.52 bb '41.33 bb 63.97 bb 300.89 bb . 50.23 M 60.05 bb 53.31 bb 56.09 bb' 296.09 bb 328 660 1251 825 781 2331 328.225 660.033 1251.132 825.343 780.944 2330.889 28.68 BM 73.83 bb 154.26 bb 96.32 bb 90.28 bb 301.19 bb 974 161 130 2166 50 2869 2955 3061 973.801 167.288 129.592 2166.195 50.002 2E69.2?0 2954.571 3060.699 116.52 bb 08.42 bb * 83.29 bb 278.18 bb 0.00 bb 374.45 bb 386.06 bb 400.50 bb ~ ~ d a l i n04o1099: : W 8 n t i t a t d and printd by m n 5/05/99 BACK TO MAIN S81nplo Liwt: I): \rat82 . P R D \ S ~ ~ l ~ \ 0 4 2 0 9 9 o h w t d r i d : mu ~ p 1r2 09:02:ii isss mthod: I):\atw2.PRO~thDP\04209Pc t u t .odLfidr N.#Y 04 01:52:40 1999 Job cod.! mintd: W d wy os 11:10:s1 1999 coapourd 2: PloB 889 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105 8aqxl.li.t Zut T0xl20, RTL, BI, Cl-ZSOppb-WD, 4/19/99 Toxl20, RTL, 1% Gl-250ppb-IUI 4/19/99 Toxl20, RTL, 133, G1-250ppb-WD. 4/19/99 Tox120, RBL04199-25Oppb-CC6, 4/19/99 MeW Blank ToxlPO, R8L04199-H2081k-lr 4/19/99 Tox120, RBLO4199-LivBlk-1, 4/19/99 Toxl20, PEL04199-3ppb- 1, I/19/99 Toxl20, RBLO4199-1Oppb-1, 4/19/99 ' Tox120, RBL04199-2Sppb-1, 4/19/99 r0x120, IleLO4199-5Oppb-1, 4/19/99 Toxl20, llI)L04199-100ppb-l, 4/19/99 Toxl20, RBLO4199-2SOppb-1, 4/19/99 Toxl20, llBL04199-500Wl. 4/19/99 Toxl20, RBL04199-750ppb-1, 4/19/99 Toxl20, IleLO4199-100-1,. 4/19/99 ryp. qC Standard Standard Standard standard. Standard standard standard Standard Sund.d 278.00 12 4 .a22 4.779 4.780 4.816 4 .E24 4.780 3.S6 4.E18 11.10 4.780 27.80 4.778 15.60 4.779 111.00 4.778 271.00 4.806 556.00 4.779 133.00 4.779 1111.00. 4.710 I : *. . . . ... . . Aru 2987 2906 2923 2396 s7 139 164 199 382 637 1058 2337 4350 6482 7651 . --2987.061 ha. 390.48 2905.830 379.43 2922.907 381.7s 2396.1S2 310.07 rlagm bb bb bb bb 56.592 ' 0.00 bb 139.347 2.98 bb 163.940 6.32 bb 14 198.881 11l08 bb 0 382.141 36.01 bb 30 637.310 70.74 bb 27 1058;178 128.01 bb 1s 2336.510 301.96 bb 9 4349.988 57s.94 w 4 6481.772 866.03 bbX 4 76SO. 592 1025.08 bbX -8 a .. .. --.i- .ud.linm 041099: :quantitatd and printd by m 5/05/99 1 . BACK TO MAIN Quantify capound -8 ?ox-120 Rat Liwr bport S q l a Limt: N:\rat#2.~\&1@~\04209~ u m t - r i d : mu Mr 22 09:02:ii 1999 Mathad: N: \rat.Z.PRO\I(.thDB\O42099a u m t d r i d : m a lay 04 oa:sz:48 i9ss Jab cod.: Printd: (IdMay 05 1l:lO:Sl 1999 cqpouad3: o w # Nuu 1 042099002 .2 042099003 3 042099004 4 042099005 5 042099006 6 042099007 7 042099008 8 042099009 9 042099010 10 042099011 11 042099012 12 042099013 13 042099014 14 042099015 15 042099016 .16 042099017 17 042099018 18 042099019 19 042099020 20 042099021 21 042099022 22 042099023 23 042099024 24 042099025 25 042099026 ~ 26 042099027 . 2 7 042099028 . 28 042099029 29 042099030 30 042099031 31 042099032 32 042099033 ' 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 42 042099043 , 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048 48 042099049 49 042099050 50 042099051 51 042099052 52 042099053 53 042099054 54 042099055 55 042099056 56 042099057* 57 042099058 58 042099059 59 042099060 60 042099061 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 66 042099067 67 042099068 68 042099069 69 042099070 70 042099071 71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 76 .042099077 77 042099078 78 042099079 79 042099080 80 042099081 81 042099082 e2 04~0990e3 83 042099084 04 042099085 85 042099086 ' 86 042099087 ai 04~099088 88 042099089 -1.li.t ?Ut @haBlank TOX120, RBLO4199-H20Elk-l, 4/19/99 tOX120, RBLO4199-LlvBlk-1, 4/19/99 ToX120, RBLO4199-5ppb-1, 4/19/99 TOXl20. RBLO4199-lOppb-1, 4/19/99 Tox120. RBLO4199-25ppb-1, 4/19/99 ToX120, RBL04199-50ppb-1, 4/19/99 Toxl7.0, RELO4199-100ppb-1, 4/19/99 TOX120, RBLO4199-25Oppb-1, 4/19/99 ToXl20, RELO4199-5OOppb-1, 4/19/99 TOXl.20, RBLO4199-75Oppb-1, 4/19/99 Tox120, RBL04199-1000ppb-1, 4/19/99 Hem Blank Toxl20. RTL. W. G1. 4/19/99 ToxlZO. RBL04199-250ppb-CC1, 4/19/99 WaOH Blank Tox120, RTL, a,Ole 4/19/99 Toxl20, RTL, m. 01. 4/19/99 Toxl20, RTL. 8U. Gl, 4/19/99 Toxizo, RTL; sn; Ci; 4ii9i99 TOXlZO, RTL; 10n. Gl,' 4/19/99 TOX120, RBL04199-25oppticQ, 4/191'99 WeOH Blank Toxl20, m L. 1W. G1. 4/19/99 TOXl20, RTL, 13U; Gl; 4/19/99 Toxl20, RTL, 14% Cl, 4/19/99 Toxl20. RTL. 1%. G1. 4/19/99 Tox120; RBLO4199k!5O&-CC3, 4/19/99 NaOH Blank ToXl20, RTL, 1% G2, 4/19/99 TOXl20, RTL, 18Il. G2, 4/19/99 Toxl20, RTL, 1%. G2, 4/19/99 TOX120, RTL, 20% 62, 4/19/99 Toxl20, RTL, 21x. 02, 4/19/99 TOX120, RBL04199-25oppbsE4, 4/19/99 WeOH Blank ' TOXl20, RTL. 2% G2, 4/19/99 TOxlZO, RTL, 23M. 62, 4/19/99 Toxl20, RTL, PIX. G2, 4/19/99 Toxl20, RTL, 2%. G2, 4/19/99 ToxlPO, RTL, 2611. G2, 4/19/99 TOXl20, ReL04199-250ppb-CC5. 4/19/99 WmOH Blank ToX120, RTL, 27N. 62, 4/19/99' TOXl20, RTL, 2%. 62, 4/19/99 ToX120, RTL, 3OU. GZ, 4/19/99 TOX120, RTL, 3W. 62, 4/19/99 TOx120, RBL04199-25Oppb-CC6, 4/19/99 n.OH Blank Toxl20. RTL. 33U. 63, 4/19/99 TOX120. :RTL. 34IL 63. 4/19/99 TOXl20, RTL, 3%; 63; 4/i9/99 TOX120, RTL, 3aC. G3, 4/19/99 TOX120. RTL. 37U. 63. 4/19/99 . T'Oxl20; qBL04199~250ppbccc7,4/19/99 N&ll 8lank T0~120, RTL, 38N, 63, 4/19/99 TOX120, RTL, 3% 63, 4/19/99 TOX120, RTL, 4 W i 63, 4/19/99 Toxl20, RTL, 4lJI. 03, 4/19/99. ToXl2Or'RTLr 4M. G3, 4/19/99 TOXlZO, RBL04199-25oP~b-CC8. 4/19/99 Toxl20, RTL, 434, G3, 4/19/99 Toxl20, RTL, 45U. 03, 4/19/99. Tox120. RTL. 4aC. G3. 4/19/99 t o x ~ o ;RTL, 4 m ; w; 4/19/99 toxl20. RBL04199-250ppMc9, 4/19/99 Elank TOX120, RTL, 6%. GI, 4/19/99 TOXl20, RTL, 6% G5, 4/19/99 T0~120a RTL. 73U. GI. 4/19/99 ToX120, RBL04199-250ppb-CCI. 4/19/99 HaOX Blank Toxl20. RTL, 7% G5, 4/19/99 ~0x120.RTL. 7an, GS. 4/19/99 TOXl20, RTL. 7%. G5; 4/19/99 Toxl20. MLO4199-25Oppb-Cc5, 4/19/99 MeOH Blank TOX120, RTL 34, G1-25Oppb-nS. 4/19/99 TOXlZO, RTL, 311, C1-25Oppb-USO. 4/19/99 ToXl20. RTL, 6n. G1-ZSOppb-US, 4/19/99 Standard Standard Standard Standard Standard Standard Standard Standard. Standard * 5.56 11.10 21.80 55.60 111.00 218.00 556.00 833.00 1111.00 4.i6a I 4.158 I 4.154 4.112 4.156 4.118 4.117 4.114 4.117 4.162 . 4.155 4.161 4.154 4.118 178.00 4.116 218.00 4.161 4.159 4.115 4.160 4.158 4.112 218.00 4.160 4.162 4.118 4.159 4.118 278.00 4.157 4.159. 4.157 4.148 4.117 4.153 , 278.00 4.155. 4.156 4.120 4.118 4.161 4.112 271.00 4.117 4.160 4.156 4.111 4.118 278.00 4.158 4.157 4.113 4.131 4.133 4.130 211.00 4.150 4.140 4.111 4.118 4.114 4.112 278.00 4.116 4.112 4.114 4.117 .4.111 218.00 218.00 4.116 4.068 4.117 4.127 4.102 4.132 4.174 4.149 4.115 4.111 4.138 4.119 4.154 4.148 4.118 --mdalina 041099::qmntitatd and printed by mea 8/05/90 -__ 99 99.365 4.87 8Q4 -12 179 179.476 9.01 8Q4 -19 502 502.222 25.67 m -8 895 895.168 45.96 )41 -17 1993 1992.760 102.63 m -8 4901 4900.550 . 252.77 bb -9 10347 ' 10347.100 534.00 bb -4 15166' 15166.480 16551 16551.121 782.14 854.34 bb mx . -6 -23 1325 1324.885 68.15 IOI 1876 1875.834 96.59 IQI ' . 965 995 607 964.554 995.322 606.564 49.54 W ' 51.13 m' 31.06 W4 5128. 5127.719 264.50 bb -5 966 7 92 823 . 854 4262 4828 ' 2338 2072 1765 1326 5008 965.683 791.578 823.11 1 853.901 4261 -949 4828.117 2337.712 2071.375 1764.636 1326.162 5008.266 49.60 bb 40.61 bb 42.24 .43.83 IQI 119.80 bb 249.03 bb 120.44 bb 106.14 bQ4 90.85 bb 68.21 bb 258.33 bb 703 816 1144 2148 495 5496 702.748 885.923 1143.722 2141.549 494.722 5495.959 36.02 M4 45.48 bb 58.79 bb 110.62 bb , 25.28 bb 283.52 bb 562. 629 584 721 331 4659 562.068 628 .a42 583.198 721.418 330.691 4638.773 28.76 bb 32.21 bb 29.88 bb 36.99 bb 16.81 m 240.29 bb 445 667 863 410 5421 * 445.452 666.912. 862.746 409.525 5426.568 22.74 bb 34.17 bb 44.29 bb' 20.88 m 279.93 bb 1268 1021 . 1716 884 1278 4754 12683 5 1 1021.017 1715. 67 1 883.895 1278.325 4754.072 61.23 W 52.46 IOT 88-33 bb 45.38 bb 65.74 bb 245.21 IPL 1086 1005.610 55.79 IO4 993. 992.997 51.01 bb 2036 445 2036.141 104.87 bb 44s.zir ' 22.13 m 646 646.280 33.11 10( 5605 5605.275 289.16 bb 4 611 515 600 534 4323 611.040 585.165 600.354 533.884 4822.879 31.29 bb 29.95 M 30.74 27.30 M bb . 1326573 1485431 569662 494912 582310 6459 245 331762 383053 302276 4714 115 6236 5869 6367 1326572.7 50 1485431.125 569661.563 494911.680 582309.750 6459.212 245.376 331762.219 3e1051.431 302275.969 4714.130 114.576 6236.4 17 5868.653 6366.802 68495.76 bb- 25553.92 bb 30066.62 bb 12.41. IQI 17129.89 bb 19778.25 bb 15607.40 bb 243.15 m BACK TO MAIN 1 ev.w 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 1 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105 'f SupldiSt T u t TOXl20, RTL, 8(, Gl-tSOppb-IUD, 4/19/99 TOXl20, RTL, 1311, G1-25Oppb-MSs1 4/19/99 ToxlZO, RTLr 1%. C1-25Oppb-WD, 4/19/99 TOX120, RBLO4199-25Oppb-CC6, 4/19/99 N O H Blank . 20x120, RBLO4199-H2OBlk-l, 4/19/99 TOX120, RBL04199-LiVBlk-1, 1/19/99 ToXlPO, RBLO4199-5ppb-1, 4/19/99 TOX120, RBL04199-lOppb-1, 4/19/99 ' Tox120, RBLO4199-25ppb-1, 4/19/99 Tax120, RBLO4199-5Oppb-1, 4/19/99 tOX120, RBLO4199-1OOppb-1, 4/39/99 Toxl20, MLO4199-25Oppb-1, 4/19/99 70x120, RBL04199-5OOppb-1, 4/19/99 Toxl20, RBLO4199-750ppb-1, 4/19/99 Toxl20, RBL04199-100opptil;' 4/19/99 gc St.nd.rd StandArd Standard StMd.Ed. Standard Standard Standard Standard Standard 211.00 5.56 11.10 21.10 ' 55.60 111.00 278.00 556.00 833.00 1111.00. .M 4.115 4.111 4.111 4.109 4.111 4.161 4.162 4.112 4.117 4.116 4.116 4.1111 4.103 4.111 4.116 4.118 .. &am 6240 7204 7649 5035 53 49 i3 126 237 597 1203' 2466 5941 11407 16607 18582 mmmpn.. 6240.164 . CMa riqa 321.94 bb 1203.939 311.11 bb 7648.546 5035.472 52.570 49.311 42.961 . 126.050 237.146 596.971 394.66 bb 259.14 w 2.45 w . 2.29 m4 1.96 w 6.25 w 11.90 bb 30.56 bb 1282.536 65.96 bb 2465.863 127.06 m4 5941.001 ' 306.50 bb 11406.154 588.71 bb 16686.936 061.35 bb 18501.650 959.16 bbx 'I -1 12 0 10 19 14 10 6 3 -14 a .'\ 1 1 .. 1'I m b l i n m 041099::quu1tltatid 8nd printd by m u W O W 9 9 1 I Quantify C-pound 8-Y Tox-120 Rat Uvar hport I Srapla Liat: W:\rnt#~.PRO\Sugl~\042099o h a t madifid: ThU Apr 22 09:OZ:ll 1999 nethod: N: \rnta2.PRO\W.UIDB\O42099a L u t modiffad: N. May 04 00:52:40 1999 Job Coda: I caapound 4: IS I # llama 1 042099002 2 042099003 3 042099004 4 042099005 Ianplalist T u t neOH BlMk Toxl20, RBu)4199-R108lk-1, 4/19/99 MX120, RBLO4199-Li~8lk-1,4/19/99 ToxlZO, RBLO4199-Sppb-1, 4/19/99 5042099006 Toxl20, RBLO4199-1Oppb-1, 4/19/99 6 042099007 ToXl20, RBLO4199-25ppb-1, 4/19/99 1 7 042099008 0 042099009 9 042099010 10 042099011 11 042099012 Tox120, RBLO4199-5opeb-1, 4/19/99 Toxl20. RBLO4199-1OOppb-1, 4/19/99 Tox120, RBLO4199-250ppb-1, 4/19/99 TOXl20, NIl.04199-5OOppb-1, 4/19/99 T0Xl20. RBLO4199-75Oppb-1, 1/19/99 12 042099013 TOXl20, RBLO4199-100Oppb-1, 4/19/99 .I 13 042099014 14 042099015 15 042099016 16 042099017 17 042099018 R.QH Blank Tox120. RTL. W, G1, 4/19/99 ToXl20, 1111, M, Gl, 4/19/99 Toxl20, RTL, 3n. G1, 4,/19/99 TOX120r RTL, UI, G1, 4/19/99 10 042099019 TOX120, RTL, %, Gl, 4/19/99 19 042099020 Toxl20. RBLO4199-250~~b-CCl4./19/99 .- 20 042099021 MeOH Blank 21 042099022 22 042099023 1 23 042099024 T0Xl20, RTL, eC, Gl, 4/19/99 TOXIZO. RTL. m, ti, 4/19/99 Toxl20, RTL, UI, GI, 4/19/99 24 042099025 25 042099026 26 042099027 27 042099028 ToXl20, RTL, 911. 61, 4/19/99 ~0x120,n n , ion, GI, 4/19/99 Tox120, RBlA4199-25oppb-CC2, 4/19/99 new Blank 28 042099029 TOX120, RTL, 1% 61, 4/19/99 29 042099030 ToXl20, RTL, 1% G l , 4/19/99 1' 30 042099031 ToX120, RTL, 14% 61, 4/19/99 . 31 042099032 * TOXl20, RTL, 1% G1, 4/19/99 f 32 042099033 TOXl20, RBLO4199-25oppb-CC3, 4/19/99 33 042099034 WaOH Blank ' 34 042099035 35 042099036 Toxizo, n n . im, 62, 4/19/99 ToX120, RTL, 18X. 62, 4/19/99 36 042099037 TOX120, RTL, 1%. 62, 4/19/99 37 042099038 38 042099039 ToXl20, RTL, 2OW. 62, 4/19/99 ToX120, RTL, 2IX. G2, 4/19/99 39 042099040 TOXlPO, ilBL04199-25oppticc4, 4/19/99 40 042099041 MeOK Blank i 41 042099042 ' Tox120. RTL. 2% 62. 4/19/99 I 42 042099043 , ToXl20, RTL; 2%; G2; 4/19/99 43 042099044 zoxizo, nn, ~(n.62, 4/19/99 44 042099045 ~0x120,nn, zsn, 62. 4/19/99 45 042099046 Toxl20, 1111, 26K. 62. 4/19/99 46 042099047 TOx120, RBL04199-.250ppb-CCS, 4/19/99 47 042099048 MeOH Blank 48 042099049 1 49 042099050 50 042099051 51 042099052 52 042099053 53 042099054 Tox120. RTL, 27n, 62. 4/19/99' Toxl20, RTL. 29X. 62. 4/19/99 ~0x120,nn. 3011. 62, 4/19/99 . T0Xl20; RTL; 3Wi 62. 4/19/99 TOXlZO. RBLO4199-250ppb-CC6, 4/19/99 MROH Blank 54 042099015 ToxI20, RTL, 33n.. G3, 4/19/99 1 55 042099056 20x120, RTL. 34% G3, 4/19/99 56 042099057. Tox120, RTL, 3%. 63. 4/19/99 57 042099058 Toxl20, RTL. 36N. 63. 4/19/99 58 042099059 Toxl20, R n , 3m. 63, 4/19/99 _ _ 59 042099060 Toxf20, RBL04199-250DDMC7, 4/19/99 60 042099061 n.c IBlank . 4 61 042099062 Tor 20. nn, 3m, a,4/19/99' 62 042099063 TO) 20. nn, 3ow, 03, 4/19/99 63 042099064 . Ton 201 RTL, 4011, G3, 4/19/99 64 042099065 Ton 20. nm, 4 ~ .a,4/19/99 65 042099066 Tor ZO..RTL. 4 ~ .a,4/19/99 66 042099067 Ton 201 R11104199-250ppb-CC8, 4/19/99 61 042099060 W . c I Blank I 68 042099069 for 20. Rn, 4311. a,4/19/99 69 042099070 Ton 20. nn. 4%, ~ 3 ,4/19/99 70 042099071 Tor 20, RTL, 4Q(. 63, 4/19/99 71 042099072 TO# 20. nn, 431, a,4/19/99 I 12 042099073 Tor 20, RBL04199-25Oppb-CC9, 4/19/99 j 7743 004422009999007745 nT.oc1 I20B,laRnkTL. 6% 65, 4/19/99 15 042099076 Tor 201 RTL, 6Q(, G5, 4/19/99 76 042099077 TOU 20, RTLr 73110 GI, 4/19/99 77 042099078 Ton 20. RTL, 74& GS, 4/19/99 78 042099079 Ton 20, RTLr 75% G5, 4/19/99 79 042099080 Ton 20, RBL04199-25Oppb-EC4, 4/19/99 I 80 0420990ai nec I Blank 81 042099082 TON 20, RTL. 77n. GS, 4/19/99 a2 0420990a3 Ton 20, 1111, leu. GS, 4/19/99 83 042099084 Ton 20, RTL. 7%. G5, 4/19/99 84 042099085 Ton 20, RBLO4199-25Oppb-CCb, 4/19/99 I . 85 042099086 I Blank I 8876 004422009999008887 88 042099089 i*I madeline 041099::quantita.t.d and priatmd by m a 5/05/99 Standard Standard Standard Standard standard Standard Standud Standard. Standard a ac a gc a a a a a a a .-->- BACK TO MAIN . - -. .- horn 7 ST Aru amaponr. a n a . n.p#ID& . 4.419 4.477 1.00 4.417 1.00 4.475 1.00 4.472 1.00 4.414 1.00 4.413 1.00 4.481 1.00 4.4.70 1.00 4.475 1.00 4.479 11168 11168.010 0.72 bb -28 14262 14262.124 0.92 bb -8 11101 17101.a16 1.10 bb 10 14220 14220.119 0.92 bb -0 14163 14162.521 0.91 bb -9 15047 15047.055 0.97 bb -3 14165 14164.e00 0.91 bb -9 12687 iz6a6.997 0.82 bb -18 13127 13121.305 0.05 bb -15 12184 12183.721 0.79 bb -21 14980 149E0.457 0.97 M4 -3 4.417 4.473 4.479 4.473 4.400 1.00 4.477 13354 13353.618 0.86 bb -14 15480 15479.964 1.00 bb 0 16743 16743.496 1.08 bb 8 16108 16107.631 1.04 bb 4 16428 16427.918 1.06 bb 6 12965 12964-158 0.84 bb -16 4.479 12592 12591.617 0.8i bb -19 4.476 12900 12900.252 0 . 8 3 bb -17 . 4.478 14767 14767.411 0.95 bb -5 4.470 . is113 13113.133 0.85 bb -15 4.476 16167 16766.613 1.08 bb 8 1.00 4.415 15329 15328.990 0.99 bb -1 4.476 I 14402 14482.320 0.93 bb -7 4.419 11815 0.76 bb -24 4.479 13252 0.86 bb -14 4.416 12072 0.78 bb -22 1.00 4.419 12820 0.83 bb -17 4.474 4.476 4.475 4.465 4.434 1.00 4.471 7100 71OO.418 0.46 bb -54 6839 6839.444 0-44 bb -56 6915 6975.442 0.45 bb -55 7301 1304.983 0.47 bb -53 7S76 1576.105 0.49 bb -51 15008 15007.997 0.97 bb -3 4.473 4.474 4.438 1.434 4.434 1.00 4.473 7420 7419.602 0.40 bb -52 8272 8272.232 0.53 bb -47 8265 8264.774 0.53 bb -47 9236 9235.608 0.60 W -40 9268 9268.021 0.60 bb -4a 14716 14716.280 0.95 bb -5 4.470 4.434 4.473 4.429 1.00 4.47a 1715 12268 10776 11197 14400 0714.930 inz60.1i4 10776.486 11196.044 14399.545 0.56 bb -44 0.79 bb -21 . 0.70 bh -30 0.72 bli -28 0.93 bb -7 4.475 4.474 4.430 4.445 4.440 1.00 4.445 16537 16537.041 1.07 bb 7 0276 0216- 272 0.53 bb -41 7711 7716.823 0.50 bb -50 9574 9574.31) 0.62 bb -38 7747 7747.233 0.50 bb -50 15240 15240.495 0.91 bb -2 1.00 4.461 4.456 4.426 4.431 4.475 4.475 7589 7588.534 0.49 bb -51 9947- 9941-499 0.64 bb -36 1531 7537.861 0481 ' 1400.613 12113 12112.729 ,14952 14951.948 0.4) bb -51 0.55.bb -45 0.79 bb -21 0.97 bb -3 1.00 4.434 4.414 4.475 4.435 4.478 0922 1921 -930 0.18 IW -42 . 9054 13112 9645 9m54.359 13111.581 9645.204 0.64 bb -36 0.85 bb -15 0.62 bb * -38 15910 15909.816 1.03 Lib 3 4.478 10254 10253.570 4.475 * 11430 11430.374 4.434 14054 14153.102 4.442 14617 14617.333 4.461 14512 14511.B14 1.00 4.449 is712 15752.205 0.66 bb 0.74 bb 0.96 IW 0.94 bb 0.94 bb 1.02 bb -34 -26 -4 . -6 -6 2 4.465 26825 26824.661 1.73 bb 73 1.473 16234 16234 -444 1.05 IN 5 4.428 16282 16201.634 1.05 PPI 5 1.00 4.453 15674 15674.282 1.01 bb 1 4.472 15710 15117.900 1.01 bb 1 4.466 17154 11154.322 1.11 bb 11 4.480 15585 15585.048 1.01 bb 1 3 BACK TO MAIN I 1 8 n.u 89 042099090 90 042099091 91 042099092 92 042099093 . 93 042099094 94 042099095 95 042099096 96 042099097 91 042099098 98 042099099 99 042099100 100 042099101 io1 0420ssioz 102 042099103 103 042099104 104 042099105 'I SgYTLz, rvpr tl-210ppb-1OD, 4/19/99 Tox120, RTL, 1M, Gl-25Oppb-IuI 4/19/99 .ToxlZO, mxizo. RTL, lM, RBLO~ ~G1S-2~50-ppb~-lSls4~0/,~19~1//9~919-/9C9Ca~ , MeOH Blank ToxlZO, RBL04199-H20B1k-lr 4/19/99 . Toxl20, RBLO4199-LlvBlk-1, 4/19/9 TOXl20, RBLO4199-5ppb-1, 4/19/99 ToX120, RBL04199-10ppb-1, 4/19/99 Tox120, RBL04199-25-1, 4/19/99 standard Standard Standard TOXl20, RBLO4199-SOppb-1, 4/19/99 standard Toxl20, RBLO4199-100-1, 4/19/99 Toxl20, RBLO4199-25Oppb-1, 4/19/99 stmdArd * standard . Toxl20. FiBY.04199-5OOppb-1, 4/19/99 SMrd Tox120, IIBLO4199-75Oppb-1, 4/19/9 standard Tox120, W4199-lOOOppb-1.. 4/19/99 Standard Itd.coM 1-00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00. Itz 4.477 4.478 4.435 4.410 4.47i 4.478 4.473 4.435 4.433 4.434 4.433 4.465 4.434 4.434 1.431 Aru 15039 14583 15562 16369 13601 17110 20729 17142 16114 17079 1708s 15941 iwoz 15262 16108 urpon8m 15038.934 145J2.552 15562.266 16367.936 13604.745 17109.813 20728.434 11141.998 16513.951 17078.783 17007.707 15944.?41 ii40z.126 15261.598 16108.356 Cona. R8pr ( D W 0.97 bb -3 0.94 bb -6 1.00 bb 0 1.06 bb 6 0.08 bb 1.10 bb 1.34 bb 1.11 bb 1.07 bb 1.10 bb 1.10 M 1.02 bb 0.99 bb 0.99 Io( 1'04 bb -12 10 34 11 7 10 . . io 2 -i -1 ' 4 . , ud.lina 041099::quantitatd and p r i n t d Y 5/05/99 Response type: External Std, Area Curve type: Linear, Origin: Exclude, Weighting: lhr, Axis trans:None 1.53e: BACK TO MAIN /' /.' .. ! I I I Response I. i I 1 I ! i '( 3 . 0. - -. .. ... -.-. . Conc; __.--_J _ 1 I 1 , Ii Coefficient of Determination: 0.991992 i Calibration curve: 7.34883 * x + 117.484 Response type: External Std,Area Curve type: Linear, Origin: Exclude, Weighting: l/x,Axis trans: None 8.28e: I 3 ! -1 .I ! 1 .. I i i I Response 1 J I 3 1 1 /' 2 I 0 I..'' ""I"" 20b.0 40b.0 60b.0 ( f' nublina 041099::quu1Ut~tmd .ad printed by w S/OS/OO ""I"" -_.%-- '"'I"" , 1 BACK TO MAIN : ..i .a "!. ... '"'. 80b.0 f. . . Conc * '1odo:o ' Quantify CZlibraUon 11.port ?ox-120 Rat Livu I w I I C.libr8UOn: W:\rat82.~\C1~rvmDB\042099a mat -rid: 14 os 09:00:19 199s Rintd: Id m y os 11:ll:lO 1999 IJ Compound 3 name:PlFwS I CCaoleibffriaciteionntcoufrDveet:e1r9m.3in6a71tio*nx:0+.959.407605733 response type: Exteam1Std,Area Curve type: Linear, Origin: Exclude, Weighting: lht, Axis trans: None ! 2.15e4 1- 3 1 1' ! i ! I i I Response t. / 1 I 1 I' I ! I I ! t I .'' e 20b.0 40b.0 60b.0 80b.0 1 ' " ' ~"'1'''' .'.'I.'.' ~"'I'''' I.'. ' u d o l i n o 041099::qu8ntitatd and ptintad by w S/05/9S *>_- BACK TO MAIN _ -- . z . Wrn 1 1 i . .. Conc I lObO.0 - - ~ - i .---I ompound 4 name: ! 2.07e , Respons BACK TO MAIN `i ! ._.4. k I t C Conc i .o L I 011 012 013 014 O h 0!6 017 018 019 --I_1..- - - -_- u d . l i n m 011099:r ~ U b L andd.printd by y. 1/03/99 .. nethoq neport Method f11ei h a t modified: Compound: : ' Printed: M:\ratr2.PROU(.th~~\042099c..db Tue m y 04 08:52:40 1 9 9 X 1: POM Wed May OS 11:10:26 1999 Compound Name; Intrmal Ref.: Quantlfy Trace: Conc. of standard.: user nr Valuer Uarr Peak Factor: Integrate window racfor: Peak Location 1 Retention T l u ( r i ~.) : n01ative netention ~k.: Tima Window (anal : Peak Selection: neawnsr ] Rmsponse Zyp.: nrsponar : C.llbr?rtion Curves 3' * Polynomlal Typ.: Point of Orlqln: Clt Helqhtinq: Axla Tranaforutlon: . Concentration Unit.: Noir. Peak-to-peak upl'ltude: I - Smooth Smoothing: Wlnddv sir. launa): Number of smooths: Smothlnq method: t Baselines Joln vglley threrholdr Reduce peak tallinq: Rairr baarllne maximum: I ! Peak Separation i DDerstwecvtersthioucladlertsh:reshold: Shoulder threrholdr 2 1 neswnse Threshold Relative height: Absolute height: Relative area: Abaoluto arrai 3 POAi [None1 TIC. Conc. A 1.000000, Disabled 1.000000 2.000 4.480, Enabled 0.000, Disabled 0.134 Mearest mternal Are- . , . Linear EXClud. 1IX None 434 . Dlaabled 3 2 20.00 so. 00 5.00 10.00 Disabled 40.00 1.50, 0.00. 1.00, 0.00, Dlrabled DIs.bled Disabled Dimbled OY ID%? r BACK! TO. MAiIN ..%_ - Pwe 1 I I .. .-\ ,. .. f' .. 8 ? .. I I I 1 I 1 i i 7 J Method Report Method file: Last aodified: compound: . Printed: N:\ratr2.PROUIrthDB\O42099c..db N e MAY 04 0(1:52:48 1 9 9 w 2rPm Wed Way 05 11:10:26 1999 Compound Nme: Internal Raf.8 QuAntify TrACO: Cone'. of Standards: User 111 Value: User Peak ?actor: Integrate ttindow ?actor: Peak Location Retention t h e I m i n s l : Relatlvr Retention TIM: Time Window lmins): Peak Selection: Rer~onse Respon8e type: Response: Calibration Curves . Polynorial Type: Point of Origin: ?it Neightingr Axis Truraforution: Concentration Units: - Noise - Peak-to-peak mpZitude: Saooth Smoothing: Wlndow sire Iscanal : Number of smotha: Smoothing uthod: Bareliner Join valloy threshold: Reduce perk tAi1ing: Raise baseline l u x i m n : . Peak SeDarAtiOn , Draw vertical threshold: Detect shoulderr: Shoulder threshold: Reswnse Threrhold hlAtiVa bbipht: Absolute height : Relative arm: Absolute a n a i DFOS [NOMI . TIC Conc. A 1.000000. I .000000 2.000 Dtrabhd 1.780, Wub1.d 0.000, Disabled 0.834 Ilearest . Linear . Exclude 1IX Bone 434 . DisAb1.d 3 2 ._20.00 50.00 3.00 10.00 Dimab1.d 40.00 1.50. 0.00. 2.00. 0.00, Di8AbJed DirAb1.d Disabled Di8.Abl.d BACK TO MAIN .* .. Method Report Method file: Last modiflad: Compound : Printed: N:\rats2.PROWethDS\O42099e.mdb TU0 May 04 08:52:48 199W 3: P M S Wed nay 05 ll:10:26 1999 ' Compound Name: Internal Ref.8 Quantify Trace: Conc; of Standards: User RP Value: user Peak ractor: Integrate Window Factor: PM [None] TIC Cone. A 1.OOOOOO, 1.000000 2.000 Disabled Peak location Re-enrion time lminrl : Relatlve Retention Tim: time Window Inins): 4.120, Cnablmd 0.000, Disabled 0.834 Peak Selection: Nearest Reswnsa hsponse Type: hsponra: Calibration Curves Extenul Araa. P0lyno.i.l Type: Point of Origin: Fit Weighting: ' Axis Transformation: linear PlClUd. l/X mna Concentration Units: Noise Peak-to-peak Upkicude: 434 . s!?s!l Smoothfnq: Window size (scans]: Number of srooths: Smoothing method: Disabled 3 2 Baselines Joln valley threshold: Reduce peak tailinq: Raise baseline maximum: Peak Separatiorv 20.00 50.00 5.00 , -. Draw vertical threshold: Detect shoulden: Shoulder threshold: 10.00 Disabled 40.00 Response Threshold Relative haight: Absoluta height: Ralativa area: Absolute arm.! 1-50, Disablmd ' 0.00, Disabled 2.00. Disabled 0.00. D i S a 1 . d d - m bYIDS8 BACK TO MAIN 94 i R '! . .. 1 I 1 I 1 J 9 1 I i i 1 1 3 Method Report Method file: Laat modified: Compound: Printed: N:\rata2.PROWeth~~\O42099~.~ Tue Udy 04 00:52:40 19990 4: IS Wed May 05 11:10:26 1999 Compound N m : Internal Ref.: Quantify Trace: Cone. or ?Itdnd.rd.: I IS INonel rrc - FiXd Uaer RF value: Uaer Peak Factor: Integrate Window Factor: 1.000000, Diaabled . 1.000000 2.000 Peak Location Retention Tima InlnaJ: Relative Retention Tima: Time Wlndow lninslc 4.480, hubled 0.000, Disabled 0.034 Peak Selection: Reswnae Neareat Reaponae Type: Reaponae: Calibration Curve. . External Ana. Polynomial T+: Point of Origin: Fit Weighting: Axia Tranaforution: linear Exdud. l/X Uone Concentration - Unit.: Noise Peak-to-peak amplitude: - Smooth Smoothing: Window air. (scam): Number of snootha: - Smoothing mthod: Baselines Join valley thrsahold: Reduce peak tdilinq: Rdase baseline M X I a u m : 434 Dladbld 3 2 20.00 50.00 5.00 Peak Separation Draw vertical threshold: Detect ahouldera: Shoulder threahold: ,$ Response Threshold 10.00 Diaabled 40.00 Relative heiqht: Ahnolute height: Relatiwe area: Absolute area) 1.50, Diaabled 0.00. Diaabled ' 2.00. M8abl.d 0.00, Diaabled . . .. 3 I I 1 1 .. I i . ..4 . . BACK TO MAIN .-_. .- Paas A. ! AMELIA062498: FACT Equipment Log 99016 Quattro II,W8E PAGE 1 DATE: 04/20/99 STUDYNUMBER Tox-120 M A m R a t Liver DESCRIPTION* 1 FACT Method: ETS-8-5.0 Injectionvolume,lO uL Cycle time: 9.0 min. Electrode: ZSpray Mobile Phase: (A)2.0 mM NH4CH3CO2 TN-A- or stndrd number (A)'99012-5-22 (E) M a l '(B) h-A-2414 I 1' J I 1 Expires: (A) 10199 Storeatroomtemp? B or N other: Guardcolumn: None ' Part number: na Lot number: na (E) 10/99 Analytical column:keystone betasil Size: 2 x 5omm Part numbec 055-70-2 Type: c18 sphere siw: 5 um Lot numbcc P7LO4 Serialnumber:04811722 Analytical parametem Drying gas (uhr):300 ESI nebulizing gas @AT15) LC Flow ratc 300 ULEmin. Back pressure: 59 bar SheathgasW)0 , :\ HPLC solveit gradient:long2 Time (min.) %B 1) 0.00 40 2) 4.5 95 . 3 6.5 95 5 ) 7.0 40 6) 9.0 stop BACK TO MAIN s f . I Tuning Method Report Method : C:\MAsSL?mX\R?iTS2.PRORo\AcQuDB\PFoS 1 Printed: Tue Apr 20`17:06:38 1999 I 1 3 -.I 1 1 i Ms .I SOURCE ( `ESP- ) i Capillary Cone Extractor , 1 RF Lens Source Block Temp. Desolvation Temp. 1 I Pressures Analyser Vacuum Set 3.00 15 5 0.2 150 250 Rdbk 2.99 -14 -6 152 258 Rdbk 1.6e-4 4.7e-3 MS1 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier Ms2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier ! .. 7/-/72BACK TO MAIN . Sage 1 Set Rdbk - 15.0 .15.0 2.03 0 0 ": - 5 7 650 S e t Rdbk 15.0 15.0 30 `32 2.0 0.0 40 39 0 30 650 FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN ..- . \ Data: 4 / 2 2 / 99 T i i Points Bwf 4 LOQ for this data set Resvauatedfm I warn Collpound -Y kport -10 List: C:\rPsmnonrat.2.PRO\lupl~\042099d fast m o d i f i d : Thu Apr 22 07:25:34 1999 w.thod: C: \robmnot\rat.2,PRO~tt1~6\04209W Last modifid: Thu Apr 22 07:23:52 1999 Job codr: Printd: Thu Apr 22 07:31:55 1999 colpoutwl 1: "' 8y.r ...ploli.t rut 1 042099106 M.Oll Blank 2 042099107 70x120. RBL04199-R2081k-l~4/19/99 3 042099108 70Xl20; PBLO4199-LiUalk-1; 4/19/99 4 042099109 70x120, RBL04199-Sppb-1, 4/19/99 5 042099110 70x1201 RBLO4199-1Oppb-1, 4/19/99 6 042099111 TOx120, RBL04199-25ppb-1, 4/19/99 7 042099112 Toxl20. RBL04199-50ppb-1, 4/19/99 8 042099113 70x120. RBL04199-100ppb-1, 4/19/99 9 042099114 70x120. RBL04199-25Oppb-1, 4I19199 10 042099115 Tox120, RBLO4199-5OOppb-1, 4/19/99 11 042099116 Toxl20, RBLO4199-75Oppb-I, 4/19/99 12 042099117 Toxl20, RBLO4199-100O~pb-1,4/19/99 13 042099111 HeOH Blank 14 042099119 T0x120r RTL, 49& 64. 4/19/99 15 042099120 16 042099121 17 042099122 . 18 042099123 19 042099124 20 042099125 zi o4zossi26 _ _ 70x1201 RTL. SOX, G4, ToxlZO. RTL. 5111. 64. T0xlZO; RTL; 52% 64; zoxizo. RTL, sm, 64, 70x170. RBLO4199-25-1. mou Biank 70x120, RTL, S U I , 64, 4/19/99 4/19/99 4/19/99. 4ii9199 4/19/99 4/19/99 22 042099127 23 042099121 TOXlZO, RTL, 5%. G4, 4/19/99 70x1201 RTL, 5m. 64. 4/19/99 i4 0 4 i o ~ ~ i T~Ox~l20. RTL, 57% 64. 4/19/99 25 042099130 , 70x120, RTL, 5811, 64, 4/19/99 26 042099131 TOX120, RBLO4199-25Oppb-CC2, 4/19/99 27 042099132 XeOH Blank 28 042099133 29 042099134 30 042099135 70x120. RTL. 59M. 04. 4119199 ~0x120;RTL; sin; GI; 4/19/99 70Xl20, RTL, 6% 64, 4/19/99 31 042099136 ?OXl20, RTL, 6% G4, 4/19/99 32 042099137 ToXl20, RBL04199-250ppb-CC3, 4/19/99 33 042099138 XaOH Blank 34 042099139 TToaxx112200, RBLOO441i9999--lHIZ2O08Blk--11., 4I/11341/9999 35 042099140 Z2O0Xxi120;, 6RBELO044i19999--LLiVvBlikk--1i,; 4/19/99 36 042099141 70x120. RBL04199-Smb-1, 4/19/99 37 042099142 m ~ i z o ,~ ~ ~ 0 4 i s s - i - & b -4i/i,s/ss 38 042099143 70x120, RBL04199-25ppb-1, 4/19/99 39 042099144 TOxl20, RBLO4199-5Oppb-1, 4/19/99 40 042099145 Toxl20. RBLO4199-LOOppb-1. 4/19/99 41 042099146 70x120. RBL04199-250pp&lr 4/19/99 42 042099147 70x120, RBL04199-500ppb-1, 4/19/99 43 042099148 70x120, RBLO4199-750ppb-1, 4/19/99 44 042099149 TOXl20, RBLO4199-1OOOppb-1, 4/19/99 BACK TO MAIN standanl Standard Standud Standard Standard Standard Standard Standard Standa,rd Qc ac ac Standard Standard Standard. Standard Stuadud Standard Standard Standard Standard Itd Coao 5.00 10.00 2s.00 so. 00 100.00 250.00 500.00 750.00 1000.00 250.00 250.00 250.00 s.00 10.00 25.00 50. 00 100.00 250.00 500.00 750.00 1000.00 Rt 4.631 4.574 4.770 4.766 4.170 4.760 4.746 4.743 4.746 4.143 4.743 4.745 4.486 4.728 4.746 4.754 4.745 4.147 4.731 4, 502 4.746 4.724 ,4.747 4.191 4.764 4.742 4. 502 4.766 4.756 4.770 4.762 4.747 4.418 4.528 4.7.68 4.766 4.764 4.763 4.765 4.766 4.763 4.768 4.750 4.75s &am 12 40 36 57 09 155 247 415 1112 2181 2976 3297 (7 12991 6082 5703 1024 2060 1057 100 I1 63 9846 11819 651 150 1011 124 772 155 189 60 917 107 26 33 51 53 111 215 391 864 1745 2459 2843 . bno n a p *DW 0.00 bb 0.91 bb 0.00 bb 5.24 bb 5 13.82 m 38 31.66 m 27 56.22 bb 12 120.05 bb 20 287.94 14( 15 575.32 bb 15 787.41 bb 5 873.36 bbx -13 13.52 bb 3470.15 bb 1619.43 m 1517.85 m 1871.64 bb 543.96 bb 273.37 bb 9 16.96 ml 1641.02 bb 2627860 bb 3172.37 bb 164.53 bb 30.42 bb. 262.59 bb 5 23.19 bb 196.94 bb 31.70 bb 40.R5 bb 6.26 bb 251.92 1p1 1 18.64 bb 0.00 bb - 0.00 bb 3.82 bb -24 4.34 bb 51 19.80 bb -21 50.35 m 1 96.32 1a4 -4 221.52 bb -11 457.44 bb -9 648.75 bb -13 751.70 w -25 3 J i Quanufy calibrauon kport calibration: C : \ r o s 8 ~ ~ 2 . m \ c u r y . ~ 1 1 \ o ~ ~ o ~ w i L u t modlfld: Thu Apr 22 O?:30:51 1999 ! Printd: Thu Apr 22 0?:31:Ss 1999 2ompound 1 name: ZZaoleibffriaciteionnt coufrDveet:e3r.m73in2a9t4io*n:x0+.93768.6985202 tesponse type: Extemal Std,Area ! Zurve type: Linear, Or&@ Exclude, Weighting: lhr, Axis trans:None I 3.77e? I 3 1 1' I f i I '1 Response I' f 1 1 i f 1 :\ . BACK TO MAIN i i I" I"" ""I"" ""I"" ' I . . . ,Conc 20b.O 40b.0 60b.0 80b.0 1000.0 I i I- ~ Masslynx Sample Llst 1 Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999 1 File Name File Text 1 042099002 2 042099003 MeOH Blank T0~120,RBL04199-H20BIk-f, 4/19/99 1 3 042099004 T0~120R, BL04199-LivBlk-l,4/19/99 4 042099005 5 042099006 TOXl20, RBL04199-5ppb-1,4/19/99 Toxl20, RBL04199-10ppb-1,4/19/99 6 042099007 7 042099008 Toxl20, RBLO4199-25ppb-1, 4/19/99 Toxl20, RBL04199-50ppb-l,4/19/99 1 8 042099009 9 042099010 10 042099011 11 042099012 Toxl20, RBL04199-1OOppb-l,4/19/99 Toxl20, RBL04199-250ppb-1,4/19/99 Toxl20, RBLO419950Oppb-1,4/19/99 Toxl20, RBL04199-75Oppb-1,4/19/99 ] 12 042099013 13 042099014 14 042099015 15 042099016 I 16 042099017 17 042099018 18 042099019 19 042099020 20 042099021 1' 21 042099022 Tox120, RBL04199-1OOOppb-l,4/19/99 MeOH Blank Toxl20, RTL, lM, G1.4/19/99 Toxl20, RTL, 2M, G1,4/19/99 Tox120, RTL, 3M, Gl, 4/19/99 Toxl20, RTL, 4M. Gl, 4/19/99 Toxl20, RTL, 5M, Gl, 4/19/99 T0~120R, BL04199-250ppb-CC1,4/19/99 MeOH Blank Tox120, RTL, 6M, GI, 4/19/99 22 042699023 Tox120, RTL, 7M, Gl, 4/19/99 23 042099024 ToxlPO, RTL, 8M, Gl, 4/19/99 24 042099025 Tox120, RTL, 9M, Gl, 4/19/99 1 25 042099026 Toxl20, RTL, lOM, G1,4/19/99 26 042099027 T0~120R, BL04199-250ppb-CC2,4/19/99 I 27 042099028 28 042099029 MeOH Blank Tox120, RTL, llM, Gl, 4/19/99 1 I 29 042099030 Toxl20, RTL, 13M, Gl, 4/19/99 30 04209W31 Tox120, RTL, 14M, Gl, 4/19/99 31 042099032 Toxl20, RTL, 15M. Gl, 4/19/99 1 32 042099033 Tox120, RBLO4199-250ppb-CC3,4/19/99 33 042099034 MeOH Blank . 1 '34 042099035 Tox120, RTL. 17M1G2,4/19/99 35 042099036 Tox120, RTL, 18M, G2,4/19/99 36 042099037 Tox120, RTL, 19M, G2,4/19/99 37 042099038 Tox120, RTL, 20M, 02,4/19/99 1 38 042099039 Tox120, RTL, 21M, G2,4/19/99 . 39 042099040 Toxl20, RBL04199-2!5Oppb-CC4.4~19/99 40 042099041 MeOH Blank 41 042099042 Tox120, RTL, 22M, G2,4/19/99 I 42 042099043 Toxl20, RTL, 23M, G2,4/19/99 43 042099044 Toxl20, RTL, 24M, 62.4/19/99 44 042099045 Tox120, RTL, 25M, G2,4/19/99 45 042099046 * Toxl20, RTL, 26M, G2,4/19/99 46 042099041 Toxl20, RBL04199-25Oppb-CC5,4/19/99 I 47 042099048 MeOH Blank 48 042099049 Tox120, RTL, 27M, G2,4/19/99 49 042099050 Tox120, RTL, 29M, G2,4/19/99 . 5 0 042099051 Tox120, RTL, 30M, G2,4/19/99 i 51 042099052 Tox120, RTL, 31M, G2,4/19/99 52 042099053 Toxl20, RBL04199-25Oppb-CC6,4/19/99 53 042099054 MeOH Blank 54 042099055 Toxl20, RTL, 33M, G3,4/19/99 I 55 042099056 Tox120, RTL, 34M, G3,4/19/99 56 042099057 Tox120, RTL, 35M, G3,4/19/99 57 042099058 Toxl20, RTL, 36M, G3,4/19/99 1 58 042099059 Tox120, RTL, 37M. G3,4/19/99 59 042099060 Tox120, RBL0419425Oppb-CC7,4/19/99 i 60 042099061 MeOH Blank 1 BACK TO MAIN Page 1 MS File Inlet File Bottle 9MINCOMB171S-POAA 9MIN-COMB171S-POAA 9MINCOMBI7IS-POAA 9MIN-COMB17IS-POAA 9MIN-COMB171S-POAA 9MIN-COMB17IS-POAA Smin 9min 9min Omin Smin 9min 9MIN-COMB171S-POAA 9min 9MIN-COMB171S-POAA 9m1n SMIN-COMB171S-POAA Smin SMIN-COMBI71S-POAA 9m1n 9MIN-COMB171S-POAA Omin 9MIN-COMBI7IS-POAA Omin 9MINCOMB171S-POAA Smin 9MIN-COMB17IS-POAA Omin 9MIN-COMB171S-POAA Smin 9MIN-COMB171S-POAA Omin 9MIN-COMB171S-POAA Omin 9MIN-COMB171S-POAA Omin 9MINCOMB171S-POAA 9m1n 9MINCOMBI7IS-POAA 9m1n 9MIN-COMBI7IS-POAA Smin 9MIN-COMBI7IS-POAA 9m1n 9MINCOMB171S-POAA 9m1n 9MINCOMB171S-POAA 9m1n SMINCOMB171S-POAA 9m1n 9MIN-COMB171S-POAA 9m1n 9MIN-COMB171S-POAA Omin 9MIN-COMB171S-POAA 9m1n 9MINCOMB171S-POAA 9m1n SMINCOMB17IS-POAA 9MIN-COMBI7IS-POAA 9MIN-COMB171S-POAA Omin Smin 9m1n 9MIN-COMB171S-POAA 9m1n 9MINCOMBI7IS-POAA 9MIN-COMB17IS-POAA 9m1n - Smin 9MIN-COMB17IS-POAA Smin 91 1 2 3 4 5 6 7 8 9 10 11 91 12 13 14 15 16 81 92 17 18 19 20 21 82 92 22 23 24 ' . 25 83 92 26 27 28 9MINCOMB171S-POAA 9m1n 29 9MIN-COMB171S-POAA . 9min . 30 9MINCOMB171S-POAA 9m1n 84 9MIN-COMB17IS-POAA Smin 92 QMIN-COMB171S-POAA Omin , 31 9MIN-COMB17IS-POAA Omin 32 9MIN-COMB17IS-POAA 9m1n 33 9MIN-COMB171S-POAA 9m1n 34 9MIN-COMB171SPOAA Qmin 35 9MIN-COMBI7IS-POAA 9m1n 85 9MIN-COMB171S-POAA 9mh 92 SMIN-COMBI7IS-POAA 9m1n 36 SMIN-COMB171S-POAA 9m1n 37 9MIN-COMB171S-POAA 9m1n 38 9MIN-COMB171S-POAA 9m1n 39 9MIN-COMBI7ISPOAA Smin 86 9MIN-COMB171SPOAA 9m1n 93 9MIN-COMB17IS-POAA 9m1n 40 9MIN-COMBI7IS-POAA Omin 41 9MIN-COMB171S-POAA 9m1n 42 SMIN-COMB171S-POAA Smin 43 SMIN-COMB171S-POAA Smin 44 9MIN-COMBIIIS-POAA Smin 87 9MIN-COMB171s-POAA Smin 93 M ~ ~ S -Is~amWple List Sample Ust: C:WASSLYNX\rats2.PRO\SampieDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999 61 62 63 64 65 66 67 68 .69 70 71 72 73 74 75 76 77 78 79 a0 ai a2 a3 84 as 86 a7 aa a9 90 91 92 93 '94 95 96 97 ga 99 I00 101 102 103 104 Flle Name 042099062 042099063 042099064 042099065 042099066 042099067 042099068 042099069 042099070 042099071 042099072 042099073 042099074 042099075 042099076 042099077 042099078 042099079 042099080 042099081 042099082 042099083 042099084 042099085 042099086 042099087 042099088 042099089 042099090 042099091 042099092 042099093 042099094 042099095 042099096 042099097 042099098 042099099 042099100 042099101 042099102 042099103 042099104 042099105 Flle Text Tox120. RTL, 38M, G3,4/19/99 Tox120, RTL, 39M, G3,4/19/99 Tox120, RTL, 40M, 03,4/19/99 Tox120, RTL, 41M, G3,4/19/99 Tox120, RTL, 42M, 03,411 9/99 Tox120, RBL04199-250ppb-CC8,4/19/99 MeOH Blank Tox120, RTL, 43M, G3,4/19/99 . Tox120, RTL, 45M, 63,4/19/99 Tox120, RTL, 46M, 03,4/19/99 Tox120, RTL, 47M. G3,4/19199 Toxl20, RBL04199-250ppb-CC9,4/19/99 MeOH Blank Toxl20, RTL, 65M, 05,4/19/99 Toxl20, RTL, 66M, G5,4/19/99 Toxl20, RTL, 73M, G5,4/19/99 Tox120. RTL, 74M, G5,4/19/99 Toxl20, RTL, 75M, 65,4119199 Tox120, RBLO4199-250ppb-CC4,4/19/99 , MeOH Blank Toxl20, RTL, 77M, G5,4/19/99 Toxl20. RTL, 78M, G5,4/19/99 Tox120, RTL, 79M. G5,4/19/99 T0~120,RBL04199-250pp~C54,/19/99 MeOH Blank T0~120R, TL, 3M, G1-250ppb-MS, 4/19/99 T0~120,RTL, 3M. 01-250ppb-MSD, 4/19/99 T0~120R, TL, 6M, G1-250ppb-MS, 4/19/99 T0~120,RTL, 6M, Gl-250ppbMSD, 4/19/99 T0xl20, RTL, 13M, 01-250ppb-MS. 4/19/99 T0~120,RTL, 13M, Gl-25Oppb-MSD, 4/19/99 ToxlZO, RBLO4199-250ppb-CC6,4/19/99 MeOH Blank T0~120R, BL04199-H20BIk-l,4/19/99 T0~120,RBL04199UVBlk-1,4/19/99 ToxlPO, RBL04199-5ppb-1,4/19/99 Tox120, RBLO4199-1Oppb-l,4119/99 Toxl20, RBL04199-25ppb-1,4/19/99 Toxl20, RBL0419940ppb-1,4/19/99 Toxl20, FU3LO4199-10Oppb-l,4/19/99 Tox120, RBLO4199-250ppb-1,4/19/99 Toxl20, RBL04199-5oOppb-1,4119/99 Toxl20, RBL04199-75Oppb-1,4/19/99 Toxl20, RBLO4199-lOOOpp~l,4/19/99 - Y / J BV AYCYK TO . MAIN mrs Page 2 MS Flle Inlet File 9MIN-COMBI7IS-POAA Qmin 9MIN-COMB171S-POAA SMIN-COMB171S-POAA 9MINCOMBI'IIS-POAA 9MIN-COMB171SPOAA 9MINCOMBITISPOAA 9MIN-COMB17IS-POAA 9MINCOMB17ISPOAA SMINCOMBI7IS-POAA 9min Smin Omin Smin 9m1n 9m1n 9m1n Omin 9MINCOMBl7lSPOAA 9MINCOMBI7ISPOAA 9MIN-COMB171S-POAA 9MINCOMBI7ISPOAA Qmln 9m1n 9m1n 9m1n SMIN-COMB171SPOAA Omin 9MIN-COMB17IS-POAA . 9min 9MINCOMBI7ISPOAA 9m1n 9MIN-COMBI7IS-POAA Omin 9MIN-COMB171S-POAA Omin 9MIN-COMB171SPOAA 9m1n SMINCOMB17IS-POAA Omin a 9MIN-COMB171SPOAA Smin 9MINCOMB171S-POAA Smin 9MIN-COMBI7IS-POAA Smin 9MIN-COMB171S-POAA Smin 9MiNCOMBCIIS-POAA SMIN-COMB171S-POAA 9MIN-COMB17ISPOAA 9MINCOMB171S-POAA 9MIN-COMBl7IS-POAA 9MIN-COMB171S-POAA 9MINCOMB17IS-POAA 9m1n Omin Smin Smin Omin Smin 9m1n 9MIN-COMB171S-POAA 9m1n 9MIN-COMB17ISPOAA 9min . 9MIN-COMBl71SPOAA 9m1n 9MIN-COMBIPIS-POAA :I 9min OMIN-COMBI7IS-POAA Smin 9MINCOMB171SPOAA Omin 9MIN-COMBI7IS-POAA 9m1n 9MINCOMBIIIS-POAA Smin 9MIN-COMBI7IS-POAA 9m1n 9MIN-COMBI7ISPOAA 9m1n 9MIN-COMB171SPOAA 9m1n 9MlNCOMB171S-POAA Smin OMINCOMBI7IS-POAA 9m1n Bottle 45 46 47 48. 49 88 93 50 51 52 53 89 93 68 69 70 71 72 . 84 94 73 74 75 85 95 76 77 78 79 80 90 86 94 1. 2 3 4 5 6. 7 8 9 10 11 1 I - Masslynx Sample Ust I Sample List: C\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL i Printed: Tue Apr 20 1651:40 1999 1 i File Name Flle Text 1 I 042099106 2 042099107 3 042099108 t .4 5 042099109 042099110 6 042099111 - 7 042099112 1 8 042099113. 9 042099114 1 10 042099115 I1 042099116 12 042099117 13 042099118 1 14 042099119 15 042099l20 16 042099121 17 042099122 MeOH Blank T0~120,RBL04194H20BIk-l,4/19/99 T0~120,RBL04199-UvBlk-l,4/19/99 Tox120, RBL041994ppb-l,4/19/99 Toxl20, RBL041941Oppb-l,4/19/99 Tox120, RBL04199-25ppb-1,4/1 9/99 Tox120, RBL041995oppb-l,4/19/99 T0~120,RBL04199-lOOpPb-l,4/19/99 Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBLO4199-75Oppb-l,4/1 9/99 Toxl20, RBL04199-100Oppb-1,4/19/99 MeOH Blank Tox120, RTL, 49M, G4,4/19/99 Tox120, RTL, 50M, 64,419199 Tox120, RTL, 51M, G4,4/19/99 Tox120, RTL, 52M,G4,4/19/99 1' 18 042099123 19 042099124 20 042099125 21 042099126 22 0420'99127 Tox120, RTL, 53M, G4,4/19/99 Tox120, RBL04199-250ppb-CCl,4/19/99 MeOH Blank Tox120, RTL, WM, G4,4/19/99. Tox120, RTL, 55M, G4,4/19/99 23 042099128 Tox120, RTL, 56M, G4,4/19/99 24 042099129 . TOX120, RTL, 57M, G4,4/19/99 ' 25 042099130 Tox120, RTL, 58M, G4,4/19/99 26 042099131 T0~120,RBLO4199-250ppb-CC2,4/19/99 27 042099132 MeOH Blank I 28 042099133 Tox120, RTL, 59M, G4,4/19/99 29 04209913, Tox120, RTL, 61M. G4,4/19/99 30 042099135 Tox120, RTL, 62M, G4,4/19/99 31 042099136 Tox120, RTL, 63M, G4,4/19/99 32 042099137 Toxl20, RBL04199-250ppb-CC3,4/19/99 33 042099138 MeOH Blank . I .34 042099139 . T0~120,RBL04199-H20BIk-l, 4/19/99 35 042099140 T0~120,RBL04199-UVBlk-1,4/19/99 36 042099141 Toxl20, RBLO4199spp~l,4/19/99 37 042099142 Toxl20, RBL04199-10ppb-l,4/19/99 1 38 042099143 39 042099144 Toxl20, RBL04199-25ppb-1,4/19/99 Tox120, RBLO4199-5Oppb-l.4119/99 . 40 042099145 .Tox120, RBL04194100ppb-1,4/19/99 41 042099146 Toxl20, RBL04199-25Oppb-1,4/19/99 1 42 042099147 Tox120, RBL04199-50Oppb-l,4/19/99 43 042099148 Tox120, RBLO4199-750ppb-l,4/19/99 I 44 042099149 Tox120, RBL04199-1000ppb-1,4/19/99 I I r'I It 1 7/-/1/ BACK TO MAIN /ncs _-_- Page 1 MS Flle inlet Ale long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 long6 . . lloonngg66 . long6 long6 long6 long6 long6 long6 . long6 long6 long6 long6 long6 ' long6 long6 long6 long6 long6 long6 s, long6 long6 ' long6 long6 long6 long6. long6 long6 long6 Bottle 91 1 2 3 4 5 6 7 8 9 10 11 93 54 55 56 57 58. 81 94 59 60 61 62 63 82 94 64 65 66 67 83 94 1' 2 3 4 5 6 7 6 9 10 11 ~ 1 t - Masslynx Sample List I Sample List: C:\MASSLYNXrats2.PRO\SarnpleDB\042099d.SPL I Printed: Tue Apr 20 16:51:40 1999 .I I i I 2 i 3 .4 5 6 -7 1 8 9 1 IO I1 12 13 1 14 15 16 17 1 18 19 20 21 22 23 24 25 26 1 27 28 i 29 30 i 31 32 33 '34 35 36 37 f 38 39 40 41 42 1 .43 3 44 File Name 042099106 042099107 042099108 042099109 042099110 042099111 042099112 042099113 042099114 042099115 042099116 042099117 042099118 042099119 042099120 042099121 042099122 042099123 042099124 042099125 042099126 0420'99127 042099128 042099129 042099130 042099131 042099132 042099133 04209913, 042099135 042099136 042099137 042099138 042099139 042099140 042099141 042099142 042099143 042099144 042099145 042099146 042099147 042099148 042099149 Flle Text MeOH Blank T0~120,RBL04199-H20Blk-l, 4/19/99 T0~120,RBL04199-UvBlk-l,4/19/99 Tox120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-1Oppb-l,4/19/99 Toxl20, RBL04199-25ppb-l,4/19/99 Tox120, RBL04199-5Oppb-1,4/19/99 Toxl20, RBL04199-1OOppb-l,4/19/99 T0~120,REL04199-250ppb-l,4/19/99 T0~120,RBL04199-5OOppb-1, 4/19/99 Tox120, RBL04199-750ppb-1,4/19/99 Toxl20, RBL04199-10OOppb-1,4/19/99 MeOH Blank Tox120, RTL, 49M, G4,4/19/99 Tox120, RTL, 50M, G4,4/19199 Tox120, RTL, 51M, G4,4/19/99 Tox120, RTL, 52M, G4,4/19/99 Tox120, RTL, 53M, 64,4119199 Tox120, RE3LO4199-25Oppb-cCl,4/19/99 MeOH Blank ToxlPO, RTL, 54M, G4,4/19/99. Tox120, RTL, 55M, G4,4/19/99 Tox120, RTL, 56M, G4,4/19/99 . T0~120,RTL, 57M, G4,4/19/99 ' Tox120, RTL, 58M, G4,4/19/99 T0~120,RBL04199-250ppb-CC2,4/19/99 MeOH Blank Tox120, RTL, 59M, G4,4/19/99 Tox120, RTL, 61M, G4,4/19/99 Tox120, RTL, 62M, G4,4/19/99 Toxl20, RTL, 63M, G4,4/19/99 Tox120, RBL04199-250ppb-CC3,4119/99 MeOH Blank . T0~120,RBL04199-H20Blk-l, 4/19/99 TOXl20, RBL04199-UVBlk-1,4/19/99 Tox120, RBL04199Sppbl,4/19/99 Tox120, RBLO4199-1Oppb-l,4/19/99 Toxl20, RBL04199-25ppb-l14/l9/99 Toxl20, RBL041945oppbl. 4/19/99 .Toxl20, RBLO4199-10Oppb-l,4119/99 10x120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19199 Tox120, RBL04199-75Oppb-1,4/19/99 ToxlPO, RBL04199-1OOOppb-l,4/19/99 1 I i 1 1 .J ~ ~~ 7/-/ BACK TO MAIN mc&r -- - Page 1 1MS Flle PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBW PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 . PFBS-4 PFBS-4 , PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBs-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS4 PFBS-4 PFBS-4 PFW PFBS-4 Inlet flle 10ng6 10ng6 10ng6 10ng6 10ng6 10ng0 10ng6 10ng6 10ng0 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 long6 . 10ng6 10ng0 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 10ng6 2 10ng6 .+ long6 long6 ' 10ng6 10ng6 10-6 lowe. 10ng6 10ng6 10ng6 Bottle 91 1 2 3 4 5 6 7 8 9 10 11 . 93 54 55 56 57 58 81 94 59 60 61 62 63 82 94 64 65 66 67 83 94 1. 2 3 4 5 6 7 0 9 10 11 1 1 I 3 1 .I I 3 J 1 1 Nootloa : 2 #Iw ot 1Icue N r ( 1 8 P 8 - . Inter Channel Delay 1 Srw 1 : 0.03 Span ( Dalton. ) : 0.00 S t a r t T f U I Win. ) I 0.00 M Tim. 1 niru 1 x 9.00 nepeeta : 1 Ch.nn.1 Parurt Daughter -11 ( S a u l C d l Energy (evl Cone ( V 1 1 Noation : 3 469.00 119.00 ,. o f 1 #.s 0.30 30 N r ( ESP- ) .... 15 .. , . I n t e r Channel Dolay ( Sew ) : 0.03 Span ( Dalton. I : 0.00 S t a r t Tima ( Win. ) : 0.00 End T i x u I Wine 1 I 9.00 Repaeta t 1 Chumel Parent Daughter Dm11 (ha)Coll Energy (e+) Cone ( V ) 1 . SC?.OO 119.00 0.30 30 15 Function I 4 IIR( of 1 #.e Rtr ( 1- 1 I n t e r Churnel Dolay ( S e w ) I 0.03 SpUl ( DaltOM ) : 0.00 S t a r t Ti.. I nina 1 : 0.00 End Time I Mine I : 9.00 Repeat6 : 1 Channel Parent Daughter -11 (Seca) Coll Energy (ev) 1 419.00 119.00 0.30 30 Cone (-V 15 Function :' 5 )(RI of 1 #.e N r ( 1- ) .. I n t e r Channel Delay ( Sece ) : 0.03 Span ( Daltoru 1 : 0.00 S t a r t Tim ( nina ) : 0.00 End Time I Win. 1 : 9.00 Rapoat. 1 1 channel Parent Daughter -11 (sees) COLI 1 519.00 119.00 0.30 30 Energy ( e n c ~ M ' (v 15 I n t e r Channel b l a y ( S e u ) : Span ( DaltOM 1 : S t a r t T ~ M( nine : mldTilM(nia0): R.peat. x ' Churn.1 Perurt Doughter 0.03 0.00 0.00 9.00 1 Rnli 1 499.00 99.06 0.30 (Sua) -11 IS Energy (evl Coru ( V ) 60 ?unatia : 7 1(1# ol1 I(.**?air ( UP-1 I n t e r churnel Dolay ( See. ) : 0.03 -Span ( Daltona ) : 0.00 s t a r t 7- ( HhJ') I 0-00 End Tim I nine 1 : 9.00 Repeat. : ' 1 Chuuul Parurt Dau. gh.ter -11 1 ' 399.00 80.00 0.30 ( 5 . u ) C o l l Energy (eV) 45 Cone 1 V 1 60 ?uaation : ## of 1 Nu. N r f 1.p- 1 I n t e r Channel Delay I S e a 1 : Span I Dalton8 J : S t a r t Tis* ( W i ~ 1i : End ti^ ( n i n i ) : Repeatr : Channel Parent Daughter 0.03 0.00 0.00 9.00 1 -11 (Seta) Coll Energy (eV) 1 427.00 80.00 0.30 40 Cone I V I 60 I . . .. . . . . .. . . I . / BACK TO MAIN .. .. BACK TO MAIN ~ - &lVNlt ( dM ) I 0.00 NnotionIl ##ofl#e.Nr(mP-) . Inter Channel Delay ( s e a I : 0.03 Spa ( Daltona : 0.00 Start Tinu ( Una ) I 0.00 B d ThO ( UM ) : 9.00 nopoatr I 1 Chennal Parent Daughter -11 ( s e a ) ~ o l al r g y (eV) Con. 1 V I - 1 413.00 169.00 0.30 30 1s nraotion : 2 ld# of 1m.# ?air t ESP- ) I n t o r Channel Delay ( stca ) I 0.03 Span ( Dalton8 1 I 0.00 Start T ~ M U M 1 I 0.00 End T f i u ( UMI I 9.00 Ropaats : 1 Channel Parent Daughter -11 (Saul Coll a r g y (*VI Con. ( V ) 1 469.00 119.00 0.30 .. 30 15 . . .. motion : I ## of 1 I(uo N r ( UP-) I n t e r Chennel Delay ( sew 1 I 0.03 Span ( Deltons ) I 0.00 1' > Start T ~ M( Hlna 1 I 0.00 End Timu I Nina : 9.00 Repeats I 1 Channel Parent Daughtar -11 1 * 560.00 119.00 0.30 (&a)Coll Energy (*VI 30 ?unotion : 4 W of 1 Mu8 Pair ( L8P- ) Intmr Chennml Delay ( Soce 1 : 0.03 Span ( Oaltom I : 0.00 S t A r t T b ( UM ) I 0.00 end Tima ( U n a ) I 9.00 Repeats : 1 hennml Parent Daughter Dwell (Secal Coll Energy (eV) 1 419.00. 119.00 0.30 30 Cone (-V ) 1s Naation r l ## of 1 #.e P.ir ( up- I .. I n t e r Channel hlay ( Sacs I : 0.03 Span I Dalton8 ) : 0.00 S t a r t T i n u ( wfna ) I 0.00 End T i n u ( nina : 9.00 Rep.ata : 1 Channel Paront Daughter -11 (Socsl Coll Enarqy lev) 1 519.00 119.00 0.30 30 Cone'( V I 15 nmationt6' w a a o ~ ~ # r m ~ r l r ( ~ . - ) . . -. .. _. . . .. / 1 499.00 99.06 0.30 45 60 NMtion : 7 ## of 1 be. N r t mP- 1 I n t e r channel -lay ( sacs 1 : 0.03 Span ( Deltons : 0.00 S t a r t Tim ( U n e ' l I 0.00 1 . 399.00 80.00 0.30 4s 60 nmotioa I 0 of 1 #.a N r t LBP- I n t a r Channel Delay ( Sacs I : 0.03 Span ( DeltOn8 I I . 0.00 S t a r t tima ( NitlJ 1 : 0.00 End tfu ( nine I 9.00 Repeats I 1 Channel Parmnt Deughtar -11 IS.cs) Coll Cnirgy IaV) Cone V ) 1 427.00 80.00 0.30 40 60 .. , 1 1 I lof-t D d 8 y t rin. 1 I 0.00 Nnation I 1 m or 4 Mu. P a i n t LII- ) Inter Channel Dalay ( S e a ) I 0.03 .. -. I SpUl [ h l t O M 1 I Start Time (.Xina I `I End Tima I Nine 1 r nap*at. : Ch.hn.1 Parent Daughter 0.00 0.00 13.50 1 & d l(Secr) all Energy (eV1 Cone. ( V I -1 299.00 `80.00 0.30 45 60 . 2 299.00 99.00 0.30 45 60 3 299.00 130.00 0.30 45 60 1 4 299.00 180.00 0.30 4s 60 I .. `1 f 1 1 1I ; . 1 ? / 7B/ 3A!C!K' TO MAIN . . - ...... . .. .. I 1. 1 f BACK TO MAIN AMELIA062498:FACT EquipmentLog 99016 Quattro II, #6448E PAGE DATE: 04/22/99 . ANALYST:= I STUDY NUMBER Tox-120 MATRIX: Rat Liver DESCRIPTION* FACT Method: ETS-8-5.0 Injection volume~l0uL Cycle&e: 13.5 min. Electrode: z-spray Mobile Phase: (A)2.0 mMNH4CH3CO2 TN-A- or stndrd numb4 '(A) ' 99012-5-22 Prepared (date/by): (A) 4/16/99 / ok (B) . (B) 'I%-A-2414 ' - (B)4/9/99 hoj Expins: (A) 10199 Storeatroomtemp? or N Guard column: None . Other: (B) 10/99 Part number: na Lot number. na Analytical column: keystone betasil Sk2x50mm T y p e : c18 Sphere size: 5 um Part number: 055-701-2 ' Lot number: P7L04 Serial number:04811722 Analytical parameters: Drying gas (I&):300 ESI nebulizinggas (L/hr) 15 LC Flow rate: 300 ~LJmin. Back pressure: 59 bar HPLC solvent gradient: long2 ' Time(min.) 1) 0.00 2) 8.5 . 3 11.0 5) 12.0 6) 13.5 YoB 40 90 90 40 . stop Sheath gas (I&)0 2 .. I Q ..< .* .. AMELIA062498:FACT Equipment Log 99016 Quattro I& #6448E PAGE BACK TO MAIN (//f499 --. Mm+ DATE: 04/22/99 ANALYST: ! STUDY NUMBER Tox-120 MATRM: Rat Liver (pfbs) DESCRIPTION2 FACT Method: ETS-8-5.0 Injection volumej10 S Cycle time: 15min. Electrode: ZSpray I Mobile Phase: (A)2.0 mMNH4CH3C02 'I"-A- or stndrdnumber (A) 99012-5-22 (B) MeOH ' (B) 'I"-A-2414 1 Prepared (datehy): Expires: . (A) 4116/99f ok (A) 10199 * (B)4/9/99 hoj (B) 10199 Store at room temp? ' fl or N other: Guard column: None * Part number: na Lot number: na Analytical column: keystonebetasil size:2 x 5omm Type: c18 Sphere size: 5 um Part number: 055-701-2 ' Lot number: F7LO4 Scrialnumba: 04811722 Analytical parameters: Drying gas (uhr):300 ESI nebulizing gas (ubr)IS LC Flow rate: 300 uL/mix~ Back pressure: 59 bar HPLC solveit gradient:long2 Time (min.) %B 1) 0.00 25 2) 5.0 95 3 12.0 95 5) 13.5 25 6 ) 15 stop Sheath gas (Llhr) 0 :\ > 1 Tuning Method Report Method: C:\MAssLYrsC\RIiTS2.PRObCQuDB\PFOS I Brinted: Thu Apr 22 16:25:24 1999 3 BACK TO MAIN -- Page 1 . ... SOURCE ( ESP- ) Capillary Cone 1 Extractor RF Lens Source Block Temp. Desolvation Temp. Set 3.00 15 5 0.2 150 250 Rdbk 2.96 -14 -5 - 152 253 Pressures Analyser Vacuum Gas Cell Rdbk 2.Oe-4 5.5e-3 MS1 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier S e t Rdbk ' 15.0 15.0 2.02 0.0 5 8 650 MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0 . 30 32 2.0 0.0 40 39 0 31 650 I 1 ~ - Masslynx Sample List 1 Sample List C\MASSLYNX\rats2.PRO\SampleDB\042299a.SPL I Printed: Thu Apr 22 17:50:31 1999 1 File Name 1 ' 1 042299001 2 042299002 3 042299003 4 042299004 I 5 042299005 6 042299006 7 042299007 8 042299008 - 9 042299009 10 042299010 11 042299011 3 12 042299012 13 042299013 14 042299014 15 042299015 '9 16 042299016 17 042299017 I' 18 042299016 I 9 042299019 20 042299020 21 04a99021 22 042299022 I 1 23 042299023 24 042299024 25 042299025 26 042299026 27 042299027 20 042299028 i 29 30 I 31 32 33 '34 35 042299035' 1 36 042299036 37 042299037 38 042299038 39 042299039 3 40 042299040 41 042299041 42 042299042 43 042299043 7 44 042299044 1 I I 1 f f I File Text MeOH Blank T0~120,RBL04199-H20BIk-l, 4/19/99 T0~120,RBLO4199-LiVBlk-i,4/19/99 T0~120,RBL04199-5ppb-l,4/19/99 T0~120,RBLO4199-1Oppb-1,4/19/99 T0~120,RBL04199-25ppb-lD4/19/99 T0~120,RBLMl99-50ppb-l,4/19/99 T0~120,RBLO4199-lOOppb-1,4/19/99 Toxl20, RBLO4199-25Oppb-1,4/19/99 Toxl20, RBL04199-5OOppb-1,4/19/99 Tox120, RBL04199-750ppb-lD4/19/99 Toxl20, RBL04199-100Oppb-l,4/19/99 MeOH Blank Tox120. RTL, 49M, G4,4/19/99, DlO Toxl20, RTL, 50M, G4,4/19/99, D10 Tox120, RTL, 51M. G4,4/19/99, DlO Toxl20, RTL, 52M, G4,4/19/99, DlO Toxl20, RTL, 53M, G4,4/19/99, DlO T0~120,RBL04199-250ppb-CCl,4/19/99 MeOH Blank Tox120, RTL, 54M. G4,4/19/99, D10 Toxl20, RTL, 55M. G4,4/19/99, DlO Toxl20, RTL, 56M, G4,4/19/99, DlO Tox120, RTL, 57M. G4,4/19/99, Toxl20, RTL, 58M, G4,4/19/99, Tox120. RBL04199-25Oppb-CC2,4/19/99 MeOH Blank ToxlPO, RTL, 59M, G4,4/19/99, . Tox120, RTL, 61M, G4,4/19/99, Toxl20, RTL, 62M, G4,4/19/99, Tox120, RTL, 63M, G4,4/19/99, Tox120, RBLO4199-250ppb-CCl,4/19/99 MeOH Blank . T0~120,RBL04199-H20Blk-1,4/19/99 T0~120,RBL04199-UVBlk-1,4/19/99 T0~120R, BL04199-5ppb-1,4119/99 Tox120, RBL0419410ppbl,4/19/99 TOXl20. RBL04199-25ppb-I, 4/19/99 ToxlZO, RBL0419950ppbl,4/19/99 T0~120,RBL04199-1OOppb-1,4/19/99 Toxl20, RBL04199-25Oppb-i,4/19/99 T0~120,RBL04199-500ppb-1,4/19/99 T0~120,RBL04199-75Oppb-l,4/19/99 T0~120,RBLO4199-1OOOppb-1,4/19/99 OL2Y ctp MS File 15MIN 15MIN 15MIN 15MIN 15MIN I5MIN 15MIN t 15MIN 15MIN 15MlN 15MIN 15MIN 15MIN 15MlN 15MIN 15MIN 15MIN ' 15MIN 15MIN 15MIN 15MlN 15MIN 15MIN 15MIN 15MIN . 15MIN 15MIN 15MIN ISMIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MN 15MIN BACK TO MAIN ~ - Page 1 inlet Flk longl5 longl5 longl5 long15 longl5 longl 5 longl5 longl5 longl5 longl5 long15 longl5 long15 longl5 longl5 long15 longl5 longl5 long15 long15 longl5 longl5 long15 long15 longl5 long15 long15 longl5 long15 long15 long15 long15 long15 long15 2 long15 "Ilong15 long15 long15 long15 long15 long15 longl5 long15 long15 Bottle 91 1 2 3 4 5 6 7 8 9 10 11 91 12 13 14 15 16 . 17 91 18 19 20 21 22 23 91 24 25 26 27 17 92 1% 2 3 4 5 6 7 8 9' 10 11 1 - Masslynx Sample List 1 Dc2llw Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042299b.SPL Printed: Thu Apr 22 17:46:29 1999 rCi I File Name I I 042299045 2 042299046 3 042299047 4 042299048 I 5 042299049 6 042299050 7 042299051 8 042299052, 9 042299053 3 I O 042299054 11 042299055 12 042299056 13 042299057 1 . I 4 042299058 15 042299059 16 042299060 17 042299061 1 18 042299062 19 042299063 20 042299064 21 042299065 22 042299066 1 23 042299067 24 042299068 25 042299069 26 042299070 I 27 042299071 28 042299072 I 29 042299073 30 042299074 I 31 32 33 .34 35 042299075 042299076 042299077 042299078 042299079' 1 36 042299080 37 042299081 .38 042299082 39 042299083 40 042299064 41 042299085 1 42 042299086 43 042299087 44 042299088 45 042299089 46 042299090 J 47 042299091 48 042299092 f 49 * 50 51 52 042299093 042299094 042299095 042299096 I 53 042299097 54 '042299098 55 042299099 56 042299100 i 57 042299101 58 042299102 59 042299103 60 042299104 File Text MeOH Blank TOXl20, RBL04199-H20Blk-l, 4/19/99 T0~120R, BL04199U~BIk-l,4/19/99 TOX120, RBL04199-5ppb-1,4/19/99 T0~120R, BL04199-1Oppb-1,4/19/99 T0~120R, BL04199-25ppb-lI4/19/99 TOX120, RBL04199-50ppb-1,4/19/99 T0~120R, BL04199-100ppb-1,4/19/99 Toxl20, RBL04199-250ppb-i,4/19/99 T0~120R, BL04199-500ppb-1,4/19/99 Toxl20, RBL04199-750ppb-1,4/19/99 TOXl20, RBL04199-1000ppb-1,4/19/99 MeOH Blank Tox120, RTL. 17M. G2,4/19/99 Tox120, RTL, 18M. G2,4/19/99 Toxl20, RTL. 19M. G2,4/19/99 Tox120, RTL, 20M, G2,4/19/99 Tox120, RTL. 21M. G2,4/19/99 T0~120R, BL04199-250ppb-CC4,4/19/99 . MeOH Blank Toxl20, RTL, 22M, G2,4/19/99 Tox120, RTL, 23M, G2,4/19/99 Toxl20, RTL, 24M, G2,4/19/99 Tox120, RTL, 25M, G2,4/19/99 Toxl20, RTL, 26M, G2,4/19/99 TOXl20, RBL04199-250Ppb-CC5,4/19/99 MeOH Blank Toxl20, RTL, 27M. G2,4/19/99 Toxl20, RTL, 29M, G2,4/19/99 Tox120, RTL. 30M, G2,4/19/99 Toxl20, RTL, 31M. G2,4/19/99 Toxl20, RBLO4199-25Oppb-CC6,4/19/99 MeOH Blank Toxl20, RTL, 33M, G3,4/19/99 Toxl20, RTL, 34M, G3,4/19/99 Tox120, RTL, 35M, G3,4/19/99 Tox120, RTL, 36M, G3,4/19/99 Toxl20, RTL, 37M, G3,4/19/99 TOxl20, RBLO4199-250ppb-CC7,4/19/99 MeOH Blank Toxl20, RTL, 38M, G3,4/19/99 Toxl20, RTL, 39M, G3,4/19/99 Tox120, RTL, 40M, G3,4/19/99 Toxl20, RTL, 41M, G3,4/19/99 Tox120, RTL, 42M, G3,4/19/99 Toxl20, RBLO4199-250ppb-CC8,4/19/99 MeOH Blank Toxl20, RTL, 43M, G3,4/19/99 Tox120, RTL, 45M, G3,4/19/99 Toxl20, RTL, 46M, G3,4/19/99 Toxl20, RTL, 47M, G3,4/19/99 T0~120R, BL04199-25Oppb-CC9,4/19/99 MeOH Blank T0~120R, BL04199-H20Blk-l, 4/19/99 TOXl20, RBL04199-LiVBlk-1,4/19/99 T0~120R, BL04l99-5ppb-lI4/19/99 Toxl20, RBL04199-10ppb-1,4/19/99 TOxl20, RBL04199-25ppb-i,4/19/99 TOXl20, RBL04199-50ppb-iD4/l9/99 T0~120R, BL04199-100ppb-1,4/19/99 1 2 I A BACK TO MAIN Page 1 US File Inlet File 13MIN-COMB171S-POAA 13MIN-COMB171SPOAA 13MIN-COMB171SPOAA 13MIN-COMBI'IISPOAA 13MIN-COMB17IS-POAA 13MIN-COMB17IS-POAA 13MIN-COMB171S-POAA 13MIN-COMBI7IS-POAA 13MIN-COMB171S-POA4 13MIN-COMB171S-POAA long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 13MIN-COMB17IS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB17ISPOAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMBI7IS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171SPOAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMBI71S-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171s-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB171SPOAA~long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB17IS-POAA long2 13MIKCOMB171SPOAA long2 13MIN-COMBI71S-POAA long2 13MIN-COMB171SPOAA long2 13MIN-COMB171S-POAA long2 13MIKCOMB171SPOAA long2 13MIN-COMB171S-POAA long2 13MlKCOMB171S-POAA long2 13MIKCOMB171S-POAA long2 13MIKCOMB171SPOAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171SPOAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMBIIIS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB171SPOAA long2 13MIN-COMB171S-POAA long2 13MIN-COMB17IS-POAA long2 13MIN-COMB171S-POAA long2 13MIN-COMBi7IS-POAA long2 Bottle 91 1 2 3 4 5 6 7 8 9 10 11 91 31 32 33 34 35. 84 92 36 37 38 39 40 85 92 41 42 43 44 86 93 45 46 47 48 49 87 93 50 51 52 53 54 88 93 55 56 57 58 89 93 1 2 3 4 5 6 7 - Mastlynx Sample Ust Sample List: C:WSSLYNX\rats2.PRO\SampleDB\042299b.SPL . Printed: Thu Apr 22 17:46:29 1999 File Name File Text 61 042299105 T0~120R, BL04199-25Oppb-1,4/19/99 62 042299106 63 042299107 64 042299108 T0~120R, BL04199-50Oppb-l,4/19/99 T0~120R, BL04199-750ppb-1,4/19/99 Toxl20, RBL04199-1000ppb1,4/19/99 i 65 i 6667 68 69 1 70 71 72 73 1 74 I' 4 / i i I I 1 1 I I j i ! 1 i 7 / * V Y 7BACK TO MAIN mt5G _-- Page 2 MS File Inlet File 13MIN-COMB171SPOAA 13MIN-COMB171S-POAA 13MIN-COMB171SPOAA 13MIN-COMB171SPOAA long2 long2 long2 long2 Bottle 8 9 ' 10 11 I I Rlntd: . Thu Apr 22 16:50:16 1999 8ol-t Delay ( riru ) : 0.00 ff mnatlon : 1 WPI of I ma. Rlr t EaP- ) 1 Inter Churnel Delay t Seca k : 0.03 Span I Dalton. 1 : 0.00 S t a r t Tim8 I nlna I : 0.00 End Tima I Xina I : 13.50 aeput. : 1 churn81 Pumnt Daughter Dwell (Sacs) Coll tnarw IW Cone t V ) 1 413.00 169.00 0.30 30 15 nmotioo : 2 IAl of 1 #ma hir ( ESP- ) 1 I n t e r Channel Delay 1 Seca 1 : Span I DaltOM ) : Start T h I nina I : End Tim8 I nina I : R.peeta : channel Parent Daughter 0.03 0.00 0.00 13.50 1 -11 tseca) C o l l k r q y b V 1 3 1 469.00 119.00 0.30 45 Cone t V ) 35 nlnctloo : 3 IAl of 1#.a Rlr ('ZSP- ) 1 I n t e r Channel Delay I Seca I : 0.03 Spur ( h l t O M ) : 0.00 S t a r t T i m t UMI : 0.00 Z d T h ( XbJ I 8 13.50 Rap8.t. : 1 Clunnel Parent Daughter Dwell tSeca1 Coll Energy (.VI 7' 1 569.00 119.00 0.30 45 Cone ( V 1 35 mnotim : 4 IQ# or i me. mir i ESP- ) i I n t e r Churnel Delay 1 S a u I I i Span t DaltOM I : s t a r t TU I H- I : End Tim I Xina I : Repaata : 0.03 0.00 0.00 13.50 1 ~unn.1 Parant ~ a u g h t e r -11 1 ~ e c a 1cou WI cone ('v I 1 419.00 119.00 0.30 45 ' 35 ?unction I 5 rn of 1mea R l r t sap- I , I n t a r Channel h l a y 1 Saca ) : 0.03 I Span t Daltonr I : 0.00 I S t a r t Tine t X ~ M1 : 0.00 EndTlrU I M ~ M 3 : ; 13.50 Rapeata : 1 I Channel Parent Daughter We11 ISocrl Coll Energy lev1 Cone I V I 1 519.00 119.00 0.30 45 35 NMua I 6 . BmI of 1.aM Pair t ESP- ) I n t a r channel h l a y t seca 1 : 0.03 Spur ( Dalton. 1 : S t a r t T i U I Mlna I : Znd T i . . (I4lunr ) : . Remata 1 Ch.nn8l Parent Daughter 0.00 0 :oo 13.50 1 Dwell tSeca1 Coll Energy lev) 1 499.00 99.0; 0.30 45 Cone I V 1 60 Nnctionr7 IAlofl#aaRir(LIO-) Inter a u n n e l h h y [ seca 1 : 0.03 sP.n t DaltOM 1 : 0.00 0.00 ! End T i . . ( M ~ N 1 : 13.50 Rewata : 1 Channel Parent Daughtar Dwell tSeca1 C o l l Enerqy lev1 Cone I V I ~ 1 ' 399.00 80.00 0.30 45 60 I n t e r Channel Delay I Seca I : 0 . 0 3 . Span ( Daltons I : 0.00 I . S t a r t Time I Win. I : 0.00 f End Tim8 t Xina ) : 13.50 i Rapeata I 1 Channel Parent Daughter W e l l ISecal C o l l Energy WV1 Cone ( V I i.i 1 427.00 80.00 0.30 40 60 - - . e .i BACK TO MAIN 1 i i 1 solvent Way ( sin. ) i 0.00 lunotioar1 I Q # o r 4 N u . . r L r r ( u P - ) ~ I 1nt.r Channel h l a y ( S e a ) : 0.03 Span ( Dalton. I : 0.00 Start Time I Min. I I 0.00 1 299.00 80.00 0,30 4s 1 2 299.00 99.00 0.30 4s 3 299.00 130.00 0.30 4s 4 299.00 180.00 0.30 ' 45 1 1 -1 1 I ! 1 * . i 1 3 1 1 If . . t 4 r i ; 60 60 60 60, '. BACK TO MAIN 1 I I1 ' 1 1 3 '1 1_ . I i mwnt Dolay t .iru 1 I 0.00 hUt&b#I81 IQ#Of4-8N=(UP-)' Inter Channel Dday I S e a 1 t Span t Dalton8 1 I Start T i . . I Xinr 1 : End T i m ( Hlns 1 t 0.03 0.00 0.00 15-00 weat8 t 1 Chum81 Parmnt Daughter Cum11 l s l u ) a l l Energy taV1 1 299.00 80.00 0.30 45 2 299.00 99.00 0.30 45 3 299.00 130.00 0.30 45 . I 299.00 180.00 0.30 45 Cone t V 1 60 60 60 60 .. BACK TO MAIN .. I Date: Analyst FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN 4 123/95 hh 0. Acceptable Linear lLOQfWthirb* + I / 6 Date: Analyst: Matrix T iPoints Dilutions3 1: FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN 5h bw 4 Analytc 4: 1 Acceptable Linear lLoQfwthitdrt.* + I I I / 2 6 BACK TO MAIN 1 _-. manury collpnud lulruy 1 1-1. list: c : \ l a s s ~ n a \ r a t . 2 . ~ ~ 0 \ ~ 1 ~ ~ \ 0 4 2 2 ~ ~ ~ Last rodifid: hi Ape 23 08:51:28 1999 wthod: c:\lassL~\ats2.PAO\H.thDB\o42299a Last modifid: F r i Ape 23 01:42:43 1999 Job cod.: I Printd: 1: I l?- hi Apr 23 08:52:38 1999 aWim1i.t wt 1 042299001 UaOH Blank I 2 042299002 3 042299003 4 042299004 5 042299005 6 042299006 Tox120, RBL04199-ii2081k-1, 4/19/99 Toxl20, RBLO4199-LlvBlk-1, 4/19/99 ToX120, RBLO4199-Sppb-1, 4/19/99 . Td%lZO, RBLO4199-1Oppb-1, 4/19/99 ToX120, RBLO4199-25ppb-1, 4/19/99 1042299001 TOX120, RBLO4199-5Oppb-1, 4/19/99 8 042299008 9 042299009 10 042299010 11 042299011 1 12 042299012 13 042299013 14 042299014 15 042299015 16 042299016 17 042299017 3 18 042299018 19 042299019 io 04iiOOOio T0~120,RBLO4199-1OOppb-1, 4/19/99 Toxl20. RBLO4199-25O~mb-1. 4/19/99 T0Xl20; RBL04199-500K&l; 4/19/99 T0Xl20, RBL04199-75Oppb-1, 4/19/99 ToXl20, RBLO4199-1OOOppb-1, 4/19/99 naon Blank TOxl20, RTL, 49U. G4, 4/19/99. DlO ' ToXl20. RTL. SOU. G4. 4/19/99. D10 Tox120; RTL; sin; GI; rii9199; D ~ O T0~120. RTL, 52U. W. 4/19/9% D10 Tox120. RTL. 5311. G4. 4/19/99. D10 Tox120; RBL04199~25~pb-CC1,4/19/99 UaOH Blank I 21 042299021 22 042299022 Toxl20. RTL. S4U. G4. 4/19/99. D10 TOxl20; RTL; 5%; G4; 4/i9/99; Dl0 Toxl20, RTL, 56U. G4, 4/19/99, D10 ToX120. RTL. 57U, GI. 4/19/99, 28 042299028 29 042299029 30 042299030 7' 31 042299031 32 042299032 33 042299033 34 042299034 35 042299035 36 042299036 I 37 042299037 XaOH Blank Toxl20. RTL, SSn, G4, 4/19/99, Tox120. RTL. 6lU. G4. 4/19/99. Toxl20, RTL, 62U; GI; 4/19/99; Tox120; RTL, 6M; G4, 4/19/99. ' ToxlZO. RBL04199-25O_~.~b-CCl.4/19/99 U~ aOH~ ~ B~i-a n.k . Toxl20, RBL04199-H2081k-l~ 4/19/99 Tox120, RBLO4199-LivBlk-1, 4/19/99 TOX120, RBL04199-5ppb-1, 4/19/99 TOXl20, RBL04 199-1Oppb-1, 4/19/99 38 042299038 Toxl20, RBLO4199-25ppb-1, 4/19/99 39 042299039 Tox120, RBLO4199-5Oppb-1, 4/19/99 1 40 042299040 41 042299041 42 042299042 43 04229904i 44 042299044 Toxl20, RBL04199-100ppb-1, 4/19/99 Tow120, RBL04199-25Oppb-1, 4/19/99 Toxl20, RBL04199-500ppb-1, 4/19/99 Toxl20, RBLO4199-750ppb-10 4/19/99 . ToXl20, RBLO4199-1OOOppb-1, 4/19/99 ryp. Standard sund.rd Standard Standard 'Standud Standud Standard standard Stuulc?fd .=. ac pc standard Standard Standard Standard Standard Standard Standard Standard standard 1tdc0~1 m 4.054 4...21_ 1 _. 4.389 1 5.00 4.381 1 10.00 4.356 1 25.00 4.373 1 10.00 4.361 100.00 4.369 1 250.00 4.355 500.00 4.366 150.00 4.366 1000.00 4.363 4.234 4.278 4.274 4..2.9_ 4 _. 4.a74 250.00 . 4.291 4.359 1 4.215 4.274 4.282 4.n1 4.401 4.363 250.00 4.364 4.254 4.386 4.390 4.386 * 4.314 q50.00 4.311 4.231 4.251 4.402 5.00 4.388 1o;oo 43 8 5 25.00 4.381 50.00 4.389 lo0;Oo 4.390 250.00 4.381 500.00 4.389 750.00 4.381 1000.00 4.389 &ua 92 84 25 49 57 143 365 689 1433 3199 4460 4981 66 2461 1456 1414 1616 490 1511 130 1218 2564 3449 1003 183 1501 82 1107 156 251 69 1313 65 88 36 39 58 137 246 cona. nag. 14.13 bb 12.75 bb ' 1 . 8 5 bb 7.01 bb 8.24 bb 22.67 bb 59.14 W 114.00,W 238.55 W 534.03 U?l 144.98 W 832.14 kRU 9.84 bb 410.47 W 242.38 1111 245.31 bQ4 269.15 M 80.78 W 251.60 W 20.51 W 202.59 W 427.10 W si5.86 w 166.49 m 29.36 bb 249.79 W ' 12.50 bb 183.95 IQI 24.18 bb 40.80 W 10.34 bb 218.42 W 9.59 bb 13.38 bb 4.81 bb 5.21 bb 8.38 bb 21.62 bb 39.98 bb 104.30 W 230.41 W 530.72 W 710.11 W 149.38 IQa tm 40 -18 -9 19 14 -5 7 -1 -11 -13 4 -16 -14 -20 4. -8 6' , -5 -25 ..". 1 I 1 Quantify Qlibntion Raport 1 Calibration: C : ~ ~ ~ a t . 2 . O R O \ C u m ~ \ 0 1 2 2 9 9 ~ Last modifid: Cri Appr 23 OE:51:34 1999 Drintd: Cri Apr 23 OE:52:40 1999 BACK TO MAIN _-_. - R-1 I CCaoleibffriaciteionntocufrDveet:e5r.m97in7a1t0io*n:x0+.979.555265572 Response type: External Std,Area I Curve type: Linear, Origin: Exclude, Weighting: l/x, Axis trans: None I 3 .. .. Q 1 1' I .. I 1 19.i\, f I. I 1 1 I ,/ f.1 0. ,,..,.,,. q . . , , . . . Conc i h l i a 062490: -vi& and qu8ntlt.t.d by kjh 4/23/99 .--- BACK TO. .MAIN .. .-\ , 1 I 1 I 3 I I : 1 J - 1 1. I ! SOUP 020199: FACT Equipment Log 99014 Quattro II, #64143 PAGE DATE: 05/14/99 ANALYST& STUDY NUMBER: Tox-120 MATRM: Rat Liver DESCRIPTION dilutions FACTMethod: ETS-8-5.0 Injection volume: 10 uL Cycle time: 9 mint Electrode: ZSprav Mobile Phase: (AI2-0IdVf N H4CH3CO2 (B) MeOH TN-A- or studrd number '(A) 99011-6-9 Prepared (datemy): (A)- (B) TN-A-2506 (B) 04/26/99drb Expires: ( A Store atroomtemp? B or N I L Other: (B) 10/99 Guard column. None Part number: na Lot number: na Analytical column: Kevstone Betasil Size:2x50mm . Type: C18 Part number: 055-701-2 Lot number P7LO4 Sphere size: 5 urn Serial numbc048 1 1752 Analytical parameters: Drying gas (LAW): 350 ESI nebulizing gas (Ih1)5 LC Flow rate: 300 ullmin, Back pressure:55 bar Sheath gas (lh)0 :\ HPLC solvent gradient: 9min-2 Time (min.1 %B 1) 0.00 40.0 2) 1.00 40.0 3) 4.50 95.0 4) 6.50 95.0 5) 7.00 40.0 BACK TO MAIN * 2. . ! i .( 1 Tuning Method Report Method: 1 Printed: C:\MASSLYNX\FACT-~.PPO\A~QUDB\PFOS F r i May 1 4 16:17:39 1999 BACK TO MAIN - Page 1 i' M s 1 SOURCE ( ESP- ) ( 'I 1 Capillary Cone Extractor 1 FW Lens Source Block Temp. Desolvation Temp. Set 3.00 60 5 0.2 150 250 Rdbk 2.98 -29 -7 153 248 I Pressures I Analyser Vacuum Gas Cell Rdbk 3.3e-5 4.3e-3, m51 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier m52 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier S e t Rdbk 14.0 14.0 2.0 f . ' 0.0 5 6 650 Set 15.0 15.0 45 2.0 0.0 40 0 650 Rdbk ' 42 40 44 - Masslynx Sample List 1 Sample List: C:wIASSLYNx\FACT-l .PRO\SarnpleDB\O5t499c.SPL Printed: Fri May 14 16:17:21 1999 BACK TO MAIN Page 1 File Nami Filelext . 05149906 MeOH Blank ~ 05149906 MeOH Blank 3 05149906 TOX-120,RBL04199-H2OBlk-2,4/19/99 1 :4 05149906 TOX-120,RBL04199-LBlk-2,4/19/99 05149906 TOX-120, RBL04199-5Ppb-2,4/19/99 7 05149906 TOX-120, RBLO4199-1Oppb-2,4/19/99 05149906 TOX-120,RBL04199-2!$pb-2,4/19/99 3 8 05149906 TOX-120, RBL04199-5Oppb-2,4/19/99 05149906 TOX-120, RBLO4199-1OOppb-2,4/19/99 l"0 05149907 Tox-120, RBL04199-25Oppb-2,4/19/99 11 05149907 TOX-120,RBLO4199-500ppb-2,4/19/g9 12 05149907 TOX-120,RBL04199-750ppb-2,4/19/99 05149907 TOX-120, RBL04199-1000ppb-2,4/19/99 05149907 MeOHBlank 15 05149907 TOX-120 23M G2 / D125 16 05149907 TOX-120 24M G2/ D l:25 17 05149907 TOX-12025M G2/ D1:25 05149907 TOX-120 26M G2 / D125 19 05149907 TOX-120,RBL04199-250ppb-CC1,4/19/99 20 05149908 MeOH Blank 21 05149908 TOX-120 33M G3 / D1:25 05149908 Tox-120 34M G3/01:25 05149908 TOX-120 35M G3 / D1:25 24 05149908 Tox-120 36M G3 / D l:25 25 05149908 Tox-120 37M G3 / D1:25 I 26 05149908 Tox-12038M G3 / D125 I 27 05149908 TOX-120 39M G3 / D1:25 28 05149908 Tox-120 40M G3 / D125 1 29 ' -30 31 32 05149908 TOX-210, RBL04199-250ppb-CC3,4/19/99 05149909 MeOHBlank 05149909 TOX-12027M G2 / D1:lo 05149909 Tox-12029M G2/D1:10 33 05149909 TOX-12030M G2/ D1:lO J X f34 05149909 TOX-12031M G2/ D1:lO 05149909 TOX-120, RBL04199-25Oppb-CC4,4/19/99 05149909 MeOH Blank 37 05149909 TOX-12042M G3/D1:10 38 05149909 TQX-120 43M G3/D1:10 05149909 TOX-120 45M 6 3 / D1:lO 05149910 Tox-12046M G3/D1:10 ' 41 05149910 TOX-12047M G31Dl:lO 42 05149910 TOX-120, RBL04199-250ppb-CC5,4/19/99 43 05149910 MeOH Blank 05149910 Tox-120, RBL04199-)-1206Ik-2,4/19/99 05149910 TOX-120,RBL04199-LBlk-2,4/19/99 46 05149910 TOX-120, RBL04199-5ppb-2,4/19199 47 05149910 TOX-120, RBL04199-1Oppb-2,4/19/99 05149910 TOX-120, RBLO4199-Wppb-2,4/19/99 05149910 TOX-120, RBL04199-5Oppb-2,4/19/99 50 05149911 TOX-120,RBWl99-100ppbP,4/19/99 51 05149911 TOX-120, RBL04199-250ppb-2,4/19/99 05149911 TOX-120, RBL04199-500ppb-2,4/19/99 05149911 TOX-120, RBL04199-750ppb-2,4/19/99 54 05149911 TOX-120,RBL04199-1M)Oppb-2,4/19/99 MS File Inlet I Bottl blume Sample7 Conc A TELMERS Srnin- 96 10 Analyte TELMERS 9rnin- 96 10 Analyte TELMERS 9rnin- 41 10 Analyte TELMERS 9rnin- 42 10 Analyte TELMERS 9rnin- 43 TELMERS 9rnin- 44 10 Standard 4.93 10 Standard 9.76 TELMERS Srnin- 45 10 Standard 24.8 TELMERS Ornin- 46 10 Standard 49.3 TELMERS 9rnin- 47 10 Standard 97.6 TELMERS 9min- 48 10 Standard 248 TELMERS 9rnin- 49 10 Standard 493 TELMERS 9rnin- 50 10 Standard 735 TELMERS 9rnin- .51 10 Standard 976 TELMERS 9rnin- 96 10 Analyte TELMERS 9rnin- 52 10 Analyte TELMERS 9rnin- 53 10 Analyte TELMERS 9min- 54 10 Analyte TELMERS 9rnin- 55 10 Analyte TELMERS Srnin- 56 10 Analyte TELMERS 9rnin- 96 10 Analyte TELMERS Srnin- 57 10 Analyte TELMERS 9rnin- 58 10 Analyte TELMERS 9rnin- 59 10 Analyte TELMERS 9rnin- 60 10 Analyte TELMERS 9rnin- 61 10 Analyte TELMERZ 9rnin- 62 10 Analyte TELMERS 9rnin- 63 10 Analyte TELMERS 9rnin- 64 10 Analyte TELMERS Srnin- 65 10 Analyte TELMERS 9rnin- 97 10 Analyte TELMERS 9rnin- 66 10 Analyte TELMERS 9rnin- 67 10 Analyte TELMERS 9rnin- 68 10 Analyte 1, TELMERS 9rnln- '69 10 Analyte TELMERS TELMERZ TELMERS TELMERZ TELMERS 9rnin- 70 9rnin- 97 Smin- 71 9min- 72 Srnin- '73 10 Analyte 10 Analyte - 10 Analyte 10 Analyte 10 Analyte TELMERS 9min- 74 10 Analyte TELMERS 9rnin- 75." 10 Analyte TELMERS 9rnin- 76 10 Analyte TELMERS 9rnin- 98 10 Analyte TELMERS 9min- 41 10 Analyte TELMERS Srnin- 42 TELMERS 9rnin- 43 10 Analyte 10 Standard 4.93 TELMERS 9min- 44 10 Standard 9.76 TELMERS 9rnin- 45 TELMERS 9rnin- 46 10 standard 24.8 10 Standard 49.3 TELMERS 9rnin- 47 TELMERS 9rnin- 48 TELMERS 9rnin- 49 TELMERS 9rnin- 50 10 Standard 97.6 10 Standard 248 10 Standard 493 10 Standard 735 TELMERS 9rnin- 51 10 Standard 976 Factor 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000' 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.0w 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 t l0lMt Dalay ( minB ) : 0.00 mnoeion : 1 mm of 3 IQ..?.it[.u.-) Inter Channel Delay ( S u a 1 I spur ( DaltoD. 1 : S t a r t T h ( Mbs 1 a 0.03 0.00 0.00 ndTb(aP.) I 3.00 Xepaau : 1 ChMMl Parent Dauuhtu D u d 1 (S.e.1 -11 1 4l3.00 113.00 0.25 30 2 413.00 163.00 ' 0.25 20 3 413.00 213.00 0.25 30 .nuW (eV) Con. 15 15 .l!i (V I PunotLonr3 IRYofl*um?.Lr[x?-) Xatet Channel Delay t Seem 1 I spur f D a l t O M 1 : S t a r t T h ( Mbs ) I mTbe(Ilku) : XW.tB I Channel Parant Daughter 0.03 0.00 0.00 3.00 1 -11 (Sees) Coll pi.r(yy (ev) 1 ' 513.00 219.00 0.40 20 ' Cone ( V 1 35 -tion : 5 =Of a w. mir [ m- 1 Inter Quyul Delay t slw ( DaltoM 1 : S t u t T h ( wku ) I ndTh(Xi~): R.p.ata : Channel P u m t Secn ) : Daushtu 0.03 0.00 0.00 3.00 1 -11 (sacs)Coll rwW (ev) . _ a 613.00 219.00 0.40 20 Nnction 1 c IRY Of a YU. [ n?-) Inter Channel Delay I S u s 1 I spur ( Daltoru : S t u t T h a ( yku ) I PldTh(Xhu)r Rwata : - C h a a ~ Pl u m t Daughtar 0.03 0.00 0.00 3.00 1 -11 * (&a)Coll Xmergy (eV) 1 427.00 60.00 0.40 40 Cone ( V 1 40 COM ( V 60 BACK TO MAIN 1 1 1 f I I 3 '"I i ! ! I t 1 1 1 1 1 i j 1' 6 FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN _-._ Anal* 4: Acceptable Linear Range: -/u 0 fl&/ .c - LOQ for this dataset c1 b-evaIuataifor Merent range? 7: S e e =at: s : \ s A c T - ~ . P R D \ ~ ~ \ o s ~ ~ ~ o ~ Last mdifiad: Man hy 17 14:14:20 1999 w.tbod: S:\?=-I. PRO~thDB\051499o k s t mdifld: llon h y 11 14:23:38 1999 J o b Coda: RLatd: ?ua hy 18 00:22:40 1999 #m 1051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 1 051499067 8 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 0514990#1 22 051499082 23 051499083 24 051499014 25 051499085 26 051499086 27 051499017 21 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499090 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 -.list M MaOH Blank naon ~ 1 . n ~ TOX-120. RSL04199-R2oBlk-2, 4/19/99 Tox-120, RBLO4199-LBlk-2, 4/19/99 TOX-120. RBLO4199-5ppb-2, 4/19/99 Tax-120, llBL04199-1oppti2, 4/19/99 Tax-120. WO4199-ZSppE-2, 4/19/99 Tox-120, RBLO4199-5Oppb-2, 4/19/99 ?ox-120, RBLO4199-10-2, 4/19/99 fox-120 RBL04199-25Oppb-2, 4/19/99 Tox-129'. RBLQ4199-5OOppb-2, 4/19/99 Tax-120. RBLO4199-15Oppb-2, 4/19/99 Tox-120. BBLO4199-100Oppb-2, 4/19/99 We- Blank TOX-120 23N G2 / D1:25 Tox-12'0 2 U G2 / D1:25 ' Tox-12'02pI G2 / D1:25 . fox-I 210 2QI G2 / Dl:25 T o r 12!0, RSLO4199-25oppb-CC1, new EIIu* TOX-I2!O 33N G3 / Dl:25 TOX-1210 34M G3 / D1:25 TOX-1210 3Sn G3 / D1:25 Tox-12:O 3QI G3 / D1:25 lox-I2!o 3 m ~3 / ~i:25 TOX-12!O 3 W ' W / D1:25 4/19/99 TOX-120 39U 63 / D1:25 ToX-120 4oW 03 / Dli25 Tox-1 20, RBL04199-25oppb-cu, 4/19/99 HaQn Blank TOX-120 zm GZ / DI:~O' Tox-1 20 2SM G2 / D1:lO TOX-120 3OM G2 / D1:lO Tox-120 3lM G2 / D1:lO Tox-120, RBL04199-25Oppb-CC4, naon Blank TOX-120 42M 63 / D1:lO TOX-120 4%4 63 / D1:lO Tox-120 4% 0 / D1:lO 4/19/99 fox-120 4Q4 G3 / D1:lO _ _ ToX-120 4'11163 / D1:lO Tax-120. RBL04199-250~~b-CCS.4/19/99 Ma08 B l k k TOX-120, RBL04199-R20B1k-2r 4/19/99 TOX-120. BBLO4199-LBlk-2, 4/19/99 TOX-120, RBLO4199-5-2, 4/19/99 fox-120, RBL04199-1Oppb2, 4/19/99 TOX-120, RBLO4199-25-2, 4/19/99 Tox-12Oi RBLO4199-5Oppb-2, 4/19/99 Tox-120. RBL04199-10oppti2, 4/19/99 Tox-120, RBLO4199-ZSOppb-2, 4/19/99 TOX-120, RBLO4199-5OGppb-2, 4/19/99 Tox-120, RBLO4199-75-2, 4/19/99 To-120. RBLO4199-1OOOppb-2, 4/19/59 BACK TO MAIN _-. . 8td con0 lli &ma COM. n a q a ulrv 5.56 11.10 27.80 55.60 111.00 278.00 ' 556.00 833.00 1111.00 218.00 278 .OO 271.00 270.00 5.56 11.10 27.80 5S.60 111.00 278.00 556.00 833.00 1111.00 5.050 5.041 5.050 5.049 5.049 5.036 5.041 5.048 ,5.050 5.048 5.049 5.049 5.049 5.048 5.050 5.050 5.050 5.056 5.049 5.048 5.049 5.049 s -050 5.041 5.049 5.050 5.050 5.048 5.049 5.041 5.049 5.048 5.049 5.052 5.041 5.049 5.051 5.050 5.049 5.050 5-.050 5.049 5.041 5.051 72 132 113 175 406 801 1123 1283 368 592 283 255 464 383 601 481 . 515 503 591 .465 598 , 485 440 268 281 326 508 263 563 214 111 288 471 81 58 113 39 139 105 249 429 831 1175 1380 71.686 132.314 113.329 115.242 406.110 800.930 1122.779 1212.724 368.311 592.299 282.603 254.921 463.685 382.606 601.330 480.556 515.341 502.511 590.622 464.784 597.567 485.149 439.186 268.097 280.175 325.677 501.135 262.546 562.991 213.511 177.013 287.691 471.073 81.083 57.712 112.981 . 38.519 138.857 104.892 248.672 429.161 837.128 1175.497 1319.191 9.88 m 78 55.49 bb 41.18 bb 81.71 bb 261.22 bb 557.95 bb 199.83 bb 920.04 bbX 232.06 bb 401.15 bb 168.40 bb 147.60 bb 304.49 bb loo -2 6 -21 . -6 0 -4 -17 . 10 243.55 .bb 401.94 bb . 317.17 bb 343.31 bb 333.72 bb 399.89 bb '105.32 bb 405.11 bb 320.62 bb 15 216.60 bb 157.50 bb 167.02 bb 200.77 bb 337.90 bb 153.32 bb 379.13 bb 116.47 bb 19.04 bb '172.22 IQI 310.04 bb 22 . 12 16.95 IQI 0.00 141 40.92 bbX 636 0.00 ml -100 . 60.37 bb 111 34.84 bb ,c -37 142.90 bb :.! 29 278.54 bb 0 SB5.15 bb 5. 839.45 bb 1 992.99 W -11 , loup 020199. R.viawd .ad q0rntiUtdbu kb 5/11/99 BACK TO MAIN g E 3 m z Z z EzZlY- .1- Liat: O r \ ~ - l . O R O \ ~ ~ \ O 5 1 4 9 9 U - a t modifid! )Im #r 17 14:14:20 19s) mthadr 8:\~-l.~\w.thDB\05149pO L u t modifiad: Non bhy 17 14:23:# 1999 Job cod.: Printdr T w M y 10 08!22:40 1999 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051199100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 Meon BlUIlk ?ox-120. RBL04199-82OBlk-2r 4/19/99 fox-120. RBLO4199-~lk-2, 4/19/99 Tox-120. RBLO4199-5ppb2, 4/19/99 ?ox-l2o, RBLO4199-1Oppb-2, 4/19/99 Tox-120, RBLQ4199-25ppb-2, 4/19/99 TU-120. RBLO4199-5Oppb-2, 4/19/99 TOx-1200 RBLO4199-10-2, 4/19/99 ToX-120, RBL04199-25Oppb-2r 4/19/99 Tax-120; RBL04199-MOppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120. RBLO4199-100Op.p.b-2, 4/19/99 xaon Blrpr Tox-120 23U 0 2 4 m:25 Tax-120 2uI GZ / Dl:25 Tax-120 2% Q / Dl:25 Tor-120 2BI Q / D1:ZS Tox-120. RBL04199-25O_mb-.CCl. hon S l d Tox-120 334 Q / Dlt25 Tax-120 34l4 03 / D1:25 4/19/99 Tox-120 3% Q / D1:25 Tor-120 3Q4 G3 / D1:25 Tor-120 37H 63 / D1:25 Tox-120 3811 03 / Dl:25 ?ox-120 3911 63 / D1:25 Tor120 (On 63 / D1:25 ?OX-120, RBL04199-25oppb-cc3, 4/19/99 nson Blank Tax-120 2 M G2 / D1:lO. Tox-120 29w 02 / D1:lO Tox-120 30n G2 / D1:lO Tox-120 3 W Q / D1:lO ?ox-120. RBL04199-25oppb-ccI~4/19/99 MeOH Blank Tax-120 42N G3 / D1:lO TOX-120 4311 03 / D1:lO lox-120 4% 03 / D1:lO Tor120 46x 63 / D1:lO Tax-120 4RI G3 / D1:lO Tox-120. RBL04199-25Oppb-CW. 4/19/99 MmOH Blank TOX-120, RBL04199-82081k-2, 4/19/99 ToX-120, RBLO4199-IIIlk-2, 4/19/99 Tor-120. RBLO4199-5ppb-2, 4/19/99 - Tor-120. RBLO4199-10~~&-2. 4/19/99 Tax-120; RBL04199-25&-2; 4/19/99 T0X-120~RBL04199-5oppti2, 4/19/99 ToX-120. RBLO4199-10-2, 4/19/99 Tox-1200 RBLO4199-ZSOppb-2, 4/19/99 Tox-120, RBLO4199-5OOppb-2, 4/19/99 TO%-120, RBLO4199-75Oppb-2, 4/19/99 Tox-120. RR,L04199-1000gpb-2, 4/19/99 pc Anal*. Anal*. Qc Alulyt. AMlyt. AMlyt. Standard standard Standard Standard standard Standard standard standard Standard 5.56 11.10 27.80 55.60 111.00 278.00 ' 556.00 833.00 1111.00 218.00 ,5.307 5.306 5.307 5.307 278.00 5.306 5.308 5.308 5.308 . 5.315 5.307 5.306 5.307 278.00 5.308 5.306 I.307 5.307 278.00 5.306 5.307 5.306 5.307 5.306 5.56 11.10 27.80 55.60 111.00 278.00 . 556.00 833.00 1111.00 613 637 509 ' 473 612.731 636.927 509.037 472.806 40 49 .. 47 44 44 35 44 43 48.048. '18.581 46.922 43.598 43.676 34.651 44.313 42.794 1080 1367 1207 1023 1079.684 1361.145 1206.705 1022.573 66 66.125 03 83.086 50 50.209 63 63.197 55 55.155 bb1 bb1 bb1 bbI . WI . WI WI mu mn mu mWn I bbI bbf bb1 bb1 mn bb1 mu mu WI i, ..'! . ..-_A- BACK TO MAIN -- S: 1011 8y.u i Oiii9906i 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 i 0514490m 8 051499068 9 051199069 10 051499010 11 051499071 12 051499072 13 051499013 14 051499074 15 051499075 16 051499076 11 051499011 18 051499078 19 051499079 20 051499080 zi 0514990ei 22 osirssoei 23 051499083 24 051499084 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499091 3e 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 41 051499101 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 xO. H Blrink Tox-120, RBL04199-82081k-2r 4/19/99 Tox-120, RBLO4199-Ulk-2, 4/19/99 Tox-120, RBLO4199-Sppb-2, 4/19/99 Tox-120, RBLO4199-loppb-2, 4/19/99 TOX-120. ML04199-25ppti2, 4/19/99 TOX-120, RBLO4199-5oppb.2, 4/19/99 TOX-120. 118104199-100ppb-2, 4/19/99 Tox-120f RBw)4199-250ppb-Z, 4/19/99 TOX-120. RBL04199-500ppb-2, 4/19/9.9 Tox-120. RBLO4199-75-2. 4/19/99 Tox-120; RBL04199-100~&b-2, 4/19/99 MeOH Blank Tox-120 2311 G2 / Dlr25 Tox-120 2 M 62 / D1:25 ' Tox-120 291 Q / Dlr25 fox-120 Z6w GZ / D1:25 Tox-120. PBL04199-25Oppb-CC1, neon ni.nlr Tox-120 33N 03 / Dl:25 Tox-120 34U G3 / Dl:25 Tox-120 391 G3 / D1:25 Tox-120 36w G3 / D1:25 Tox-120 334 G3 / D1:25 Tox-120 311w G3 / D1:25 Tox-120 3% G3 / D1:25 ToX-120 4DDlG3 / D1:25 4/19/99 TOX-120, RBL04199-25~CC3, 4/19/99 Kea B1-k TOX-IZO 2m Q / ~i:i6 TOX-120 2% GZ / D1:lO Tox-120 3ON GZ / D1:lO TOX-120 3ll4 G2 / D1:lO Tox-120. RBL04199-250ppb-CC4, 4/19/99 x.oR Blank _ _ Tox-120 4ZM G3 / D1:lO ?ox-120 4 M 63 / D1:lO TOX-120 4% G3 / D1:lO fox-120 46w 03 / D1:lO ?ox-120 4RI G3 / D1:lO Tw-120. RBLO4199-25Od-CC5. neon el& ~ 4/19/99 Tox-120, RBLO4199-820Blk-2, 4/19/99 Tox-120, RBLO4199-IBlk-2, 4/19/99 Tox-120, RBLO4199-5ppb-2, 4/19/99 ?OX-120, RBLO4199-1@pb-2, 4/19/99 ?ox-120. RBLO4199-2Sppb-2, 4/19/99 Tox-120 . RBLO4199-5oppb.2, 4/19/99 Tox-120: RBLO4199-1Ooppb-2, 4/19/99 TOX-120, RBLO4199-25Oppb-2, 4/19/99 Tox-120. RBLO4199-5OOppb-2, 4/19/99 Tox-120, MLO4199-7soppb-2, 4/19/99 TOX-120. RBLO4199-100Oppb-204/19/99 ntd COM Aru 5.56 11.10 27.~0 55.60 111.00 278.00 556.00 833.00 1111.00 278.00 218.00 278.00 278.00 5.466 5.463 5.464 5.464 5.465 5.500 5.464 5.514 5.515 5.513 5.514 5.514 5.515 5.514 5.515 5.516 5.516 5.523 5.515 5.514 5.515 5.514 5.514 5.514 5.514 5.115 5.515 5.514. 5.515 5.512 5.514 5.514 5.515 5.56 11.10 21.eo 55-60 111.00 278.00 556.00 833.00 1111.00 5.516 5.515 5.513 5.514 5.516 5.515 5.513 5.516 13 34 49 128 253 . 461 163 164 13.419 34.422 .40.961 127.672 253.440 466.900 763.341 164.046 10.23 1p1 33.18 lU4 49.01 1pI 135.10 bb 212.54 bb 505.e3 bb 829.00 bb 830.51 bbX 19 78.520 ei.39 1p1 103 103.445 108.62 bb 59 se.190 59.82 1p1 86 06.441 90.03 bb 245 244.e4i 263.15 bb 90 76 66 ., 69 11 49 66 64 241 09.556 93.44 bb 76.319 79.04 1(w 65.982 67.61) nn 69.190 . 71.19 1pI 70.905 13.06 bR4 40.149 . 66.234 64.294 48.95 1pI 61.95 1pI 65.113 1p1 241.294 259.21 bb 136 135.119 143.89 bb 173 113.100 184.15 bb 116 116.489 122.17 bb 138 137, eoe 146.11 bb 275 274.145 295.83 bb 111 110.694 116.54 bb 121 121.136 127.95 bb 68 67.i4e 69.61 1pI 93 93.064 91.21 bb 71 71.230 13.41 1pI 244 244.465 262.14 bb -e __19 -12 22 -2 -9 0 -25 -5 -1 - /6 1 -5 - . 15 14.143 11.68 M 5 23 23.309 21.04 l44 -24 50 50.399 50.65 IQI -9 116 116.114 122.46 bb:? 10 2 62 262.095 282.00 bb 1 541 546.872 593.23 bb 1 762 76i.m 128.19 bb -1 773 772.968 840.32 -24 .. BACK TO MAIN bm 1 051499061 2 051199062 -allat Tut n a ~i.nlr Ir.aol Blank rup. AMlyt. AMlyt. std con0 3 051499063 Tax-120, RBL04199-mOBlL-2, 4/19/99 AMlyt. 4 051499064 Tor-120, -199-WIlk-2, 4/19/99 AMlyt. 5 051499065 Tox-120, RBLO4199-5ppb-2, 4/19/99 Standard 5.56 6 051499066 1 051499067 e 05149906e TOx-120, RBLO4199-10ppb-2, 4/19/99 TOX-120, RBL04199-25ppb-2, 4/19/99 TOX-120. RBLO4199-5-2, 4/19/99 Standud standard Standud 11.10 eo 55. 60 9 051499069 TO%-120. R8LO4199-10-b-2. 4/19/99 Standard 111.00 10 051499010 Tox-120; RBL04199-25@2; 4?19/99 11 051499071 Tox-l2UI RBLO4199-500ppb-2, 4/19/99 6t-d Standud 278.00 ' 556.00 12 051499012 Tax-120. RBLO4199-75-2. 4/19/99 Standud 833.00 13 051499073 Tox-120; VBLO4199-lOO&b-2, 4/19/99 st.ld.rd 1111.00 . 14 051499074 15 051499075 16 OS1499076 17 051499071 ie 0514sso-to MoOR Blank Tox-120 2311 G2 / D1:25 TO%-120 24M U / D1:ZS Tox-120 2% U / D1:25 TaX-120 ZQ4 G2 / Dl:25 AMlyt. AMlYt. - 5.671 5.671 5.671 44 44.162 15 14.e2s 21 20. eo9 19 051499019 ?OX-120, RBL04199-250ppb-CClr 4/19/99 21e. 00 20 osi4990eo 21 osi49soei 22 051499082 23 0514990e3 24 0514ssoe4 25 051499085 2s osi499oes 27 0514ssoei 28 os1499oee MaoB Blank T&-~ZO 3311 w o m 5 Tox-120 341 63 / D1:25 ToX-120 391 G3 / D1:25 Tox-120 3 M 63 / DI:25 ~0x-1203714 w ~ 1 : ~ s TO=-120 38n 63 / Dlt25 Tox-120 391( G3 / Dl:25 ToX-120 4OX 63 / D1:2S 29 05i4ssoes Tox-120. RBL04199-25oppticC3, 4/19/99 211.00 30 051499090 &OX Blank 31 051499091 32 051499092 33 051499093 34 051499094 Tox-120 224 G2 / D1:lO' ToX-120 2911 G2 / D1:lO Tox-120 3OM G2 / D1:lO 'lox-120 3111 G2 / D1:lO 5.672 5.672 5.611 5.612 45 45.418 10 70.121 45 44.525 41 41.121 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 Tox-120. RBLO4199-25Oppb-CCl. n a BZU* Tox-120 4 M 63 / D1:lO Tox-120 4311 63 / D1:lO Tox-120 491 03 / D1:lO TOX-120 ISH 63 / D1:lO mx-izo 4 m w ~ i : i ~ 4/19/99 TO%-120, RBL04199-25oppticC5, 4/19/99 ~ L BWlank oc AMlYt. 2711.00 210.00 44 051499101 Tox-120, FkLO4199-~aSlk-2, 4/19/99 4s 051499105 Tox-120, RBLO4199-LBlk-2, 4/19/99 Anal%* . AMlyt. 46 051499106 Tox-120. RBLO4199-5-2; 4/19/99 Standard 5.56 41 051499101 TO%-120, RBLO4199-loppb-2, 4/19/99 Standard 40 051499108 TOX-120. RBLO4199-25ppb-2, 4/19/99 Standard I 49 051499109 TO%-120; RBLO4199-5oppb-2, 4/19/99 Standud 50 osirsiiio TOX-120; RBLO4199-100ppb-2, 4/19/99 Standard 51 051499111 Tox-120, RBLO4199-250ppb-2, 4/19/99 standard 52 051499112 ToX-120, llBLO4199-5OOppb-2, 4/19/99 Standard 53 051499113 Tox-120, RBLO4199-750ppb-2, 4/19/99 Standard 54 051499114 Tox-120, RBLO4199-1OOOppb-2, 4/19/99 1 1 1 I 1 3 '1 1 ! ; i i f ! 1 I i 1 I 1 1lq1m Li8t: r : \ ~ - 1 . ~ \ ~ . D a \ O S 1 4 9 9 a k m t rodifid: mn #y 17 14:14:20 1999 hthod: 8 :\ M - l .Pm~thDn\OSl499a k8t rodifid: b b l b #Y 17 14:23:30 1999 Job cod.: Drintdr SUI wy ia 0'8:22:40 isms 5: I4613 I) N U 1 051499061, 2 051499061! 3 05149906I~ 4 051499064I 5 05149906!i 6 051499066i 7 051499067I e 05149906aI 9 051499069I io 0514ss07aI 11 051499071, 12 051499072I 13 0514990731 14 0514990741 15 051499071I 16 051499076i 17 051499077 18 051499078I 19 0514990791 20 051499080I 21 051499081 22 051499082 23 051499083, 24 051499084 21 0514ssoe5I 26 0514ssoe6 27 0514ssoe.i 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051199095 36 051499096 37 051499091 3e 0514ssosa 39 0514990991 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 05i4ssioe 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 t.1.- raxt XeOHBlank X.cUfBlu* ?ox-120. RBLO4199-BZOBlk-2, 4/19/99 ToX-120, RBLO4199-Ulk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 TOX-120. RBLO4199-1Oppb-2, 4/19/99 To%-120, RBLO4199-25ppb-2, 4/19/99 Tox-120, RBLO4199-50-2, 4/19/99 TOX-120, RBL04199-10Oppb-2, 4/19/99 TOX-120. RBLO4199-Z5Oppb-Z, 4/19/99 Tox-120, RBLO4199-5OOppb-21 4/19/99 ?OX-120, RBLO4199-75Oppb-2, 4/19/99 TOX-120. RBLO4199-1OOQpob-2, 4/19/99 . WOW8 1 m k ?ox-120 2 a . W / D1:25 Tox-120 2 u I 0 / D1:25 Tox-120 2% W / Dlr25 Tor-120 281 W / D1:25 lox-120, R8L04199-2SOppb-CC1, 4/19/99 X.WBlu* Tox-120 3% G3 / Dl:25 ToX-120 3uI G3 / D1:2S ?OX-120 3% G3 / Dlt25 Tox-120 3QI 03 / Dl125 lox-120 37I4 G3 / D1:25 ~ox-12031114 ~3 ~ 1 : ~ s ToX-120 3% 63 / Dl:ZS Tax-120 4oW 63 / D1:25 Tox-120. RBL04199-2SOppb-CC3, 4/19/99 . H a Blu* iox-izo zm GZ / DI:IO Tox-120 ZSX GZ / D1:lO Tox-120 30X 02 / D1:lO Tox-120 3lX W / D1:lO Tox-120, RBLO4199-250ppb-CC4, 4/19/99 Weoll Blank ?ox-120 4 W 63 / D1:lO .- Tox-120 4% 63 / D1:lO Tox-120 4% W / D1:lO lox-120 46M 03 / D1:lO ~ o x - 1 ~40714 w ~i:10 .~ox-izo.R B L O ~ ~ ~ ~ - ~ ~ O D D4~/1-9C/9C9S , M.M.~ ~H ~ a-i~ &.. TOx-120, RBLO4199-112081k-2, 4/19/99 Tor-120. RBL04199-LBlk-2. 4/19/99 Tox-120; RBLO4199-5ppb-2; 4/19/99 Tox-lZo, RBLO4199-1Oppb-2, 4/19/99 Tor-120. RBLO4199-25ppb-2, 4/19/99 Tox-120, RBLO4199-5Oppb-2, 4/19/99 Tox-120. RBLo4199-looDDb-2, 4/19/99 TOx-120, RBL04199-250bb&2; 4/19/99 TOx-120, RBLO4199-SOOppb-2, 4/19/99 ?ox-120, RBLO4199-75Oppb-2, 4/19/99 'Lox-120, RBLO4199-100Oppb-21 4119/99 rup. Arulyt. Arulyt. ArulyC. Arulyt. Standard standard Standard Standard Standard standard Sturdud standard standard -yt. E%: -yt. Aruliltyt. qc Grsi hlYtb st&rd stdard Standard Standard Standard St&d Standard Standard Standard 5.56 11.10 27.80 . 55.60 111.00 278.00 556.00 e33.00 1111.00 27e.00 278.00 z7e.00 278.00 5.56 11.10 n.eo 55.60 111.00 27e.00 556.00 833.00 1111.00 5.027 5.780 5.719 5.779 5.~16 5.mo 5.828 5.e~o 5.ezs 5.830 5.829 5.~30 5.829 5.830 5.810 5.m 5.829 5.831 laup 020199, RNi-md ud quratitatd e drb I/l7/99 BACK TO MAIN 22 31 56 131. 251 375 410 21.920 37.96 W 37 30.955 57.72 la4 4 56.391 113.36 1QI 2 130.633 275.75 bb -1 25i.zes 539.65 bb -3 375.092 810.45 bb -3 410.169 8ai.ie mx -20 142 142.146 300.93 bb 0 131 130.756 276.01 bb -1 150 149.709 317.47 bb 14 152 151.621 321.65 bb 16 . 14 13.778 20.15 W -28 26 26.376 47.70 IMr -14 52 51.950 103.64 BQf; -7 137 137.099 ze9.es bb. 4 286 2es.9811 615.56 bb 11 375 375.333 8io.se bb -3 419 413.935 906.35 W -18 .. , BACK TO MAIN ~ 3 r n Z E Ezzz--' .1- Urt: 8:\PACT-l.?RD\~.D.\0514990 k a t POdifiad: bbn #y 17 14:14:20 1999 m.thod: 8:\IXX-I.?RD~ebo.\0514990 k a t e l i d : Man #y 17 14:23:38 1991 Job cod.: Printd: N a #y 18 08:22:40 1999 capound(: U # m 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 7 051499067 8 051499068 9 051499069 10 051499070 ii osi4s907i 12 051499072 13 051499073 14 osi4sso74 IS 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 051499081 22 osi4ssoe2 23 0514ssoe3 24 051499084 25 osi4ssoe5 26 051499oe6 27 051499087 21 0514990ee 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 3e 051499098 391 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45, 051499105 46, 051499106 47 051499107 481 051499108 491 051499109 50I 051499110 51 051499111 52 051499112 53I 051499113 54 051499114 88mplali.t h.t Na-.O..ll Blank ~~ H M Blank TOX-120. RBL04199-E2OBU-2, 4/19/99 Tox-120, RBLO4199-LBlk-2, 4/19/99 Tox-120, RBLO4199--2, 4/19/99 TOX-120, RBLO4199-1Oppb-2, 4/19/99 Tox-120, RBL04lSS-ZSppb-2, 4/19/99 Tox-120, RBLO4199-Soppb-2, 4/19/99 Tax-120. RBLO4199-1OOppb-2, 4/19/99 Tox-120, RBLO4199-ZSOppb-2, 4/19/99 Tox-120. RBLO4199-5Ooppb-2, 4/19/99 TO%-120, RBLO4199-75-2, 4/19/99 Tox-120, RBL04199-100ogPti2, 4/19/99 neon BI- lox-120 23t GZ / D1:ZS To%-120 2 U E2 / D1:ZS . Tax-120 291 GZ / D1:ZS To%-120 2Q4 G2 / D1:ZS TOX-120, RBL04199-2Soppb-cQ~ 4/19/99 Noeotl Blank Tox-120 3351 63 / D1:25 Tox-120 3 U G3 / D1:S Tox-120 3P( 63 / D1:25 To%-120 364 63 / Dl:25 tox-120 3711 63 / D1:ZS lox-120 3QI W / D1:25 TOX-120 3% W / D1:25 To%-120 4OM G3 / D1:ZS Tox-120, RBL04199-25opeb-ccl, 4/19/99 Mea Blank TOX-120 27M G2 / D1:lO Tox-120 2% G2 / D1:lO TOX-120 3ap( 02 / D1:lO TOX-120 3lM 62 / Dl:10 Tox-120. RBL04199-25Oppb-CC4, 4/19/99 MeOR Blank TOX-120 4 M G3 / D1:lO Tox-120 4351 03 / D1:lO Tox-120 494 G3 / D1:lO Tox-120 4bn G3 / D1:lO TOX-I20 47w G3 / D1:lO TOX-i20, R B L O ~ L S ~ - ~ ~ O P ~ ~4/-1C9C/9S9, Maw Blank TOX-120. RBL04199-1(2OBlk-2, 4/19/99 TOX-120, RBLO4199-LBlk-2, 4/19/99 Tow-120. RBLO4199--2, 4/19/99 Tox-120, RBLO4199-loppb-21 4/19/99 Tox-120, RBLO4199-25-21 4/19/99 ToX-120. RBLO4199-5Oppb-2, 4/19/99 ToX-120. RBLO4199-1OOppb-2, 4/19/99 TOX-120, RBLO4199-25OPpb-2, 4/19/99 To%-lZO, RBLO4199-5Ooppb-2, 4/19/99 TOX-120. RBLO4199-75Oppb-2, 4/19/99 fox-120, RBLO4199-1OOoppb.2, 4/19/99 zyp. Arulyt. 8td coria hlyt. AMI*. An3yt. standard 1.00 standard 1.00 St8ndal-d stmd.rd 1.00 1-00 . S t . n d u d 1.00 St.nd.rd 1.00 Stmd.rd 1.00 Standud 1.00 Stuukrd 1.00 -ye. AMlyt. Arulyt. Arulyt. a*. pc 1.00 hlyt. Arulyt. Arulyt. Arulyt. &Id*. Anal*. sg: hlYt. ac hlyta , 1.00 2%: -*e Arul** ac AMlyt. a*. -1s. 1-00 Analyt. AMlyt. AMI*. ac 1.00 Arulyt. Arulyt. Anal*. stmdrrd 1.00 St.nd.rd 1.00 St.adUd 1.00 st* 1.00 standard 1.00 Standard 1.00 StUldUd 1.00 Standard 1.00 Standard 1.00 RT 5.036 5.035 5.036 5.036 5.033 5.036 5.034 5.035 5.023 5.034 5.034 5.036 5.034 5.035 5.035 5.035 5. 034 5.036 5.036 5.036 5.042 5.035 5. 034 5.035 5.035 5.037 5.034 5.035 5.036 5.036 5.034 5.035 5.034 5.035 5.034 5.035 5.038 5.034 5.035 5. 037 5.036 5.035 5.036 5.036 5.035 5.034 5.037 -a CQM. n.9. WmV 2407 2406.723 0.90 bb -2 2496 2496.454 1.01 bb 1 2699 2699.373 1.09 bb 9 2722 2722.3e2 1.10 bb 10 2446 2446.267 0.99 bb -1 2280 2280.384 0.92 bb -e 2566 2565.513 2352 . 2351.564 1.04 bb 0.95 bb 4 -5 2400 2408.171 0.98 bb -2 2309 230e.6~ 0.94 bb -6 2302 2301.694 0.93 bb -7 81 109 74 70 2336 81.237 109.041 74.247 69.750 2336.227 0.03 bb -97 0.04 bb -96 0.03 m -97 0.03 BM -97 0.95 bb -5 170 81 71 . 95 84 . e3 97 73 2253 169.517 96.582 72.744 2253.416 0.07 bb 0.03 bb 0.03 BM 0.04 bb 0.03 bb 0.03 bb 0.04 bb 0.03 lQ4 0.91 bb -93 -97 -97 -96 ' -97 -97 -96 -97 -9 163 198 184 179 2464 163.472 198.362 ie3.888 178.796 2464.372 0.07 bb -93 0.08 bb -92 0.07 bb -93 0.07 bb -93 1.00 bb 0 214 213.740 0.09 bb -91 175 174.571 0.07 bb -93 , 164 163.908 0.07 bb -93 170 is9.64e 0.07 bb -93 161 160.737 0.07 bb '93 2414 2413.697 0.98 bb -2 2416 2396 2542 2751 2500 2337 2586 2464 2333 2355 2457 2415.6e3 2395.952 2541.736 2751.209 2500.397. 2336.569 25e5.568 2463.607 2333.406 2355.073 2457.479 0.98 bb -2 0.97 bb -3 1.03 bb 3 1.12 bb 12 1.01 bb 1 0.95 bb 4 -5 1.05 bb J: 5 1.00 bb ' * 0 0.95 bb -5 0.95 bb -5 1.00 Ql 0 Calibration: I: \ n c Z - 1 . ~ \ ~ \ 0 5 1 4 P s a Laat Wid: to. b y 18 00:21:03 1000 Priam h. b y 10 01:22:53 1000 >ompound1 name:POAA ZZaoleibffriactiieonntc'oufrDveet:e1rm.33in0a5t9io*nx:0+.95984.0573959 tesponse type: External Std, Area : w e type: Linear,Origin: Exclude, Weighting: lk Axis trans: None 1 S4e3 BACK TO MAIN 1 Response 636 '-%Conc X X -37.3 t X - I . , , " , " , , ' ~ " ' ' v 'x I.S!.,.... , ' " ' " ' ' ,.,, ,.,, 20b.0 40b.0 ,,E, ,,,, ,,,, ,,,/ 60b.0 , , , , ," I(,, 80b.0 , . . . 'IC- onc 1odo.o 1 1 c.lib.rtion: #:\-l .ollo\CllmD.\Wl499a k a t a r i d : nay io OI:21:03 iooe Printdr Tu. b y IO 00:12:53 1999 ?ompound 2 name: M463 1 Z2aoleibffriaciteionntcoufrDveet:e0rm*ixna+ti0on: 0.000000 Kesponse type: External Std, Area 2urve type: Linear, Origin:Exclude, Weighting: lk, Axis trans: None I 100 1 3 I Response i i I 1 i 1 i 0. I -4 .t 1 . '. %Con1 t ! i I I 1 -1 . . r 8 r 1 , . , , ,,,,,,,,, , , , , I , , . , .,,, ,,,. ,,,, .,., .,.,, ,,., I' ' 110 210 3!0 410 I 510 6!0 I 710 8!0 i BACK TO MAIN .r.r .'(. * . , , Conc 9!0 ' 1d.o. . BACK TO MAIN --. CalhUon: B : \ M - l . ~ \ ~ \ 0 5 1 4 S S o L u t lodifidr hu my 10 00:21:03 190s Printd: Turn May 10 00:22:53 1SSS I )ompound 3 name: h4513 zzaoleibffriaciteionntcoufrDveet:e0rm.91in5a0t1io9n*: 0x.9+945.80851752 tesponse type: External Std,Area b e type: Linear, Origin:Exclude, Weighting: lhc, Axis trans: None 1.02e? 1 1 1 \ Response 1' I ! 0 >,,'l""l,,'. i 21.' X ,.,, ,.(, ) ) , , (,, 1""1""1""1 1i x : .& -f. . 3 X 1 %Con( X -I ' , ' , ' , , , 1 ' " ' ,.,, , , ( , ,.,, ,,,, ,,,, , ,, , , , , I X I X X I X i 1 -25.2 X >"'I"" ""I"" I,.. .I., I,,, ..,, ,,,, I , , , ,,,, 1' 20b.0 40b.0 60b.0 80b.0 1odo.o ; 1 BACK TO MAIN 1 1 Calibration! 8 : \ M l X - 1 . ~ \ ~ \ 0 5 1 4 9 0 0 lut modifid: hu May 11 Ol:21:03 1999 Printdt Tuo #u 1I OI:22:53 1999 ?ompound 4 name: Ms63 1 z3aoleib6rcaiteionnt coufrDveet:e0rm*inxa+ti0on:0.000000 'yE": Xesponse External Std,Area 1 3urve type: hear, Origin: Exclude, Weighting: lk, Axis trans: None 1 3 3 i 1 i 1I I . s'? . 1 1 1- %Con( I 1 I I . . -1 F , , , , , , , , , , I , ,,,, , , ( , ,.,( , , , , , I , , (,,, ,,,, ( I , I ,,, , Conc i. !o 1 1 2!0 I . 3!0 410 5!0 610 7!0 I 8:o'" ' ' ' '9:o' ' ' ' 1d.o. + ~OmDOUnd5 name: M_6_ 1_ 3_ cloekicient of Determination: 0.994865 claIibration.curve:0.457183 x 4.56671 Xesponse type: External Std, Area 3urve type: Linear,Origin:Exclude, Weighting: lht,Axis trans:None 5 1; BACK TO MAIN Response 0, 36.i 'X %Cone X -27.5 .. " , , 1 ' , ' , ,,,, 20b.0 40b.0 ,,,, ,,,, 60b.0 80b.O . I I , , , , , , C o n c 1odo.o J 1 rc.u C l r h t i O 8 t : I : \ ~ l . O W \ Q u r r D . \ O 5 1 4 9 P o k a t U f i d : w M y 1I 00:21:01 1999 Rintd: zu. 18 w:22:s3 1999 ?ompound 6 name: IS Xesponse Factor: 2467.17 1 e: External Std,Area . % hXestpyonpse : 1 2.75e: 1 3 7I' Response 1 I BACK TO MAIN j I 0 11.: _I 1 1 '-%Con( 1 I i -7.57 I .t X X x X K 1 1 I I I I I I I X X .. Conc 0:1 012 013 014 0:s 016 017 0!8 019 o1: . , ? cclpound Nu.: Intmxml Raf.: Quantify Trace: conc. of standard.: User W Value: User P a d Tactor: Iataqrate Window ?actor: Peak l o u t i o n htuition T l u Was): rielatitre Retoation' Tiam: r i m window (rfnr): Peak Selection: Ramnse Raw=-. WP.: Ra8FaIl8.r Calibration Curm8 Polyamial rypa: Point of Origin: P i t Weiqhtlnq: Uti8 T r . a r f o r u t i o n r Concantration hits: - Noise Peak-to-peak upiitude: - smooth - -thinq: *indov 8 h . O ( I - ) : Number of nooths: sawthlaq m a w . Baseline8 Join r r l l a y threshold. Raduca paek tailinq: h i r e baseline ruian: Peak Semration Draw vartical threshold: h t e c t shouldmrsz Shouldar threshold: .' Reawnae Thrashold h l a t i v a heiqht: Abaolute hright: , Relative ere.: Abaolute area: &. A 1.000000, 1.000000 2.000 Dimad 4.998, SMb1.d 0.000, Di.8bl.d 0.400 19.uut LiWU meluda WX Mom 140 .. Dis8bl.d 3 '2 20.00 100.00 0.00 10.00 Di8rbl.d 40.00 1.50, D i s 8 b l . d 0.00. D i . . b J . d 1.00, D188bl.d eo.ool Bubld BACK TO MAIN 1 Hathod Raport 1 xathod f i l a : Last d f i a d : Co~urbd: S:\?AcT-1.PROWathDB\0S1499c.Bdb Hon Buy 17 14:23:38 19990 z; 11463 Printed) Tua Buy 18 08:41:45 1999 1 Compound Name: Internal Rat.: Cuntify Traca: Conc. of 5t.nd.rd.r X463 tN0a.l TIC CanC. A 1 mer n? va1w: Usar Paak hctor: Inteqrata W i n d o w Tactor: Paak location 1.000000, Dimblad 0.000000 2.000 I Ratantion T h e (r2ruI: Ralative Rateation T i m : T i . . W i n d o w (r2ru): Paak Solaction: 5.406, l3Ubl.d 0.000, Di.8bl.d 0.551 nauut 3 Ramma Ra.pon..Zyp.: , h.rpans.: , Calibration Curas EXtuTd hrm . 1 P o l m a 1 Type: Point of origin: F i t Weighting: Axis Transfooartion: Concantration unit.: 1' P*ak-to-p.ak upiitud.: Smooth - B.wthing: i Window size ( ~ C M D ) : * 140 Disablad 3 m a r of mootha: 2 aOothin9 uthod: I Baselines I Join vallay t h ~ ~ h o l d : Raduce ped trilinq: Raise bas. l i M rulmn: 20.00 100.00 0.00 I .' Peak SeDaration .* Draw Vertical thrMbld: 10.00 Datact shoulders: Shoulder thruboldr Diarblad 40.00 I Ra-m Zbraahold Ralativa bdghtr Bbaolut- h-iqhtz xa1ativa ua.: Abaolute uu: J 1.50. Disablad 0.00, Diaabl-d . 2.00, D i m b l e d 80.00. bublad 3 t .I 1 1 i 1 rJ261M BACK TO MAIN Page 2 .A -.'.? . 1 1 1 I I 3 I 7 1 1 1 i 1 I 1 I I I 1 f i I Compound Name: I n t e n u l Rat.: Quantify Trace: C0nC. Of st.nd.rdr: User M Value: User Pmak Factor: Integrate Window ractor: Peak Loution Fatelltion ?* Imiall: Relative Rateation T U : T i U W i n b o w (-1: Peak se1ectiOrI: Farpolu. Type: na.poru.: Calibration Curves PolyIIcdal Type: Point of Origin: lit lightinp: Axis TrwfozmtiOrI: Concentration units: &gJ-e Peak-to-paak mpiltuder - smooth Smothing: window sire (scan61I Ihnb.r o f neath.: smoothing ruthod: Baseline. Join valley threshold: Reduce peak tAi1lng: naira h e . 1LM rub: Peak Swamtion Draw nrticll threshold: h t e c t shoulderr: Shoulder threshold: \. Re5wnse Thrarhold Relative height: Abnolutm height: Relative area: Abmlute areal 1.000000, D L . b l d 1.000000 2.000 5.538, Rubld 0.000, DiJ.bl.d 0.315 Wrest *.nul Aru8'. , . 140 . Di8.bl.d 3 2 20.00 100.00 0.00 10.00 D15.bl.d 40.00 1-50, Disabled 0.00. Di..blsd 2.00, D b a b l d 80.00. bubld BACK TO MAIN Compound Nw: Intern1 Rot.: qruntify ZTacm: corm. of sturd.Ed.r &. A 1.000000, 1.000000 2.000 M8.bl.d 3.349, -1.d i 0.000, Diaabld 0.511 1 Is..t..t 1 1 i J 140 t i DiM1.d 3 2 20.00 100.00 0.00 i 10.00 Diaabld 40.00 1 1.50. M8abl.d I 0.00. M8abl.d . 2.00, Di8.bl.d 1 80.00, B.bl.d I i I I i I I' I I BACK TO MAIN Metbod Report nethod rile: Last rodifid: Coqouad: Printed: S:\SXC?-1.PROWethm\O5149Sc.rdb Won Nay 17 l4:23:31 lS9W 5: x6u ma Nay 10 00:41:46 1999 ~ospoundti-: 1ntB-l h f . 8 Quantify Trace: cone. or stwrdr: 0a.r IW Vduer Waer Peak h c t o r : Inteqrate W i n d o w Factar: It613 Iworul TIC Conc. A 1.000000, 1.000000 2.000 Diaabld Peak location m t m t i o n T i u -1: m1atiw R.tantion T i u : Tk. window [dM): s.e!j3, -1.d 0.000. Dimbled 0.275 Peak Selection: Noareat R..Wn8* R.-. zyp.1 b..poM.: Calibration Curvoa Poiynort.1 ~yp.: Point of Oriqin: F i t Weighting: Axla T r u u f o n u t i o n : Concentration I Unit.: - Peak-to-pork mpiitude: s.looth 5.oothiaq: Window sir. ( E C W I : Number o f nooths: sloothin9 uthod: 140 ' Disabled 3 2 Baeelines Join valley threshold: Reduce peak t a i l i a q : mise bmleline WIimn: Peak Swaration 20.00 100.00 0.00 .! Draw v e r t i a l threshold: Dater% ahoul&rs: Shoulder threahold: b8POMe Threshold , 10.00 Disabled 40.00 Relative hiqht: Abaolutr height: R.l.ti*. area: Absolute area: , 1.50, Diaabld 0.00. Disab1.d 2.00, Dirabled 10.00, Embled 1 149 BACK TO MAIN page 5 mthod bP* Method f i l e : last m d i f i e d : s:\rAcT-l.PMw.thDB\os1499c.rdb Hon t h y 17 14:23:38 19S c4mpound: 6: IS . Printed: Tu. 1I.y 18 08:41:46 1999 colllpouad uaM: Is I Internal Rot.: rmnei Quantify Trace: TIC COW. or s t r a d u d r : Tixd user M Velm: 1.000000, Dimb1.d User Peak Factor: 1 .oooooo i Integrate Window Factor: 2.000 Peak location R o t e n t h Tfru [rim)$ R e l a t i n Retention T i m : ti.. Window ~mirm): 5.014, mld 0.000, Diarbled 0.200 P.ak Selection: BTe-at 1 RO6WEI. R o s p o ~ oTypo: RaspoM.: calibration curves rntd A?- . PolynorL.1 Typo: Point of origin: rit Weighting: Axis Trumfomation: LiMu wlclud. l/% mum Concentration 1 - Wta: Pmek-to-peak amplitude: - smooth Smoothing: window size ~ s c a M ) I Number of aPoths: -0thhg Utbod: 140 . Di6.bl.d 3 2 Baselines Join valley threshold: Raduca p u k tailing: k i n . baselin8 IPulaum: 20.00 100.00 0.00 J Peak Smaration D r i w v a r t i u l threshold: Datect should8rs: Sbarldar thraaholdr 3, 10.00 Disabled 40.00 R e m n s e Threshold Rolatin bight: Abaolutm b i g h t : Rolative am&: Ab.olute area: 1 1.50. D i s a b l d 0.00. D i s a b l d 2.00, Dfs.blOd '80.00. Bubld I .. BACK TO MAIN BACK TO MAIN I 7 SOUP 020199: FACT Equipment Log 99014 PAGE Quattro II, #6414E t DATE: 05/21/99 Uo$t\/77 I ANALYST& STUDY NUMBER:TOX-120 M A m . R a t Liver DESCRIPTION Dilutions/Tclmer . FAcTMeth~d:nS-8-5.0 Injection volume: 10 uL Cycle time: 9 min. Electrode: ZSDrav 3 TN-A-or stndrdnumber .(A) 99011-6-9 `1 Prepared (datdby): (A)- ' @) nL4-2540 (B) 05/21/99 kih I Storcatroomtemp? @ or N Other: I i Guard columd: None I Part number: na I. j Lot number: na AnaIyticalcohunn:Kevstone Betasil i Size: 2 x 50 mm Type: C18 Sphere size: 5 urn Part number: 055-701-2 Lot n u m k wL04 Serial numbcr:04811752 `.5 ?* . Analytical parameters: Dryinggas (Llhr): 350 ESI nebulizing gas (Uhr) 15 Lc Flow rate: 300uumin, Back pressure: 55 bar Sheath gas (LAX) 0 :\ HPLC solvent gradienk hnin-2 5) 7.00 40.0 Tuning Method Report Method: C:\MASSLnisr\FACT-1.PRO\ACQ~B\PFOS2 Printed: F r i May 21 16:28:16 1999 BACK TO MAIN 1 f f 1 SOURCE ( ESP- 1 Capillary Cone Extractor F S Lens Source Block Temp. Desolvation Temp. Set Rdbk 3.01. 40 5 0.2 150 250 3.01 -46 -8 153 254 Pressurea Analyser Vacuum Gas Cell Rdbk 2.4e-4 4.8e-3 .. MS1 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier LM Resolution HM Resolution Collision Ion Energy Ion mer& Ramp Lens 8 . Lens 9 Multiplier set Rabk 14.0 14.0 2.0 ;? 0 . 0 '.- . 5 3 650 ' Set Rdbk i5.o 15.0 45 . 43 2.0 0.0 40 39 0 43 650 - ~ Masslynx Sample List i Sample List: C:\MASSLYNWACT-l .PRO\SampleD8\052199b.SPL Printed Fri May 21 16:2803 1999 File Name File Text 1 052199068 MeOH Blank 2 052199069 MeOH Blank 3 052199070 TOX-120, RBL04199-H20Blk-lI 4/19/99 4 052199071 Tox-120, RBLO4199-LBIk-l,4/19/99 i 5 052199072 TOX-210, RBL04199-5ppb-1,419/99 6 052199073 TOX-120, RBL04199-1OpPb-l,4/1 9/99 1 7 052199074 8 052199075 052199076 l"0 052199077 11 052199078 TOX-120, RBL04199-25ppb-l14/19/99 * TOX-120,RBL04199-50ppb-1 419/99 TOX-120, RBL04199-100PPb-1,4/19/99 TOX-210, RBLO4199-25OPpb-1,4/19/99 TOX-120, RBLO4199-5OOpPb-1,4/19/99 1 :12 052199079 052199080 052199081 15 052199082 .i ::- 1 6 19 052199083 052199084 052199085 052199086 TOX-210, RBL04199-750Ppb-1,4/19/99 TOX-120, RBLO4199-1Oooppb-1I 419/99 MeOH Blank Tox-120 49M 0 4 I D1:lo TOX-120 50M G4I D1:lo TOX-12051M G4 ID lA0 Tax-120 52M G4I D1:lO TOX-120, RBL04199-25Oppb-CC1,4/19/99 I 20 052199087 MeOH Blank 21 052199088 TOX-12053M G4I D1:lO 22 052199089 TOX-120 54M G4I D1:lo 1 23 052199090 TOX-12055M G4/ D l:lo 24 052199091 TOX-120 56M G4 I D l:lo 25 052199092 TOX-120, RBL04199-250ppb-CC3,4119/99 i 26 052199093 MeOH Blank 27 052199094 . TOX-120 65M G5 I Dl500 28 052199095 TOX-120 66M G5 IDl500 29 052199096 TOX-120 67M G5 I Dl500 30 052199097 TOx-120 68M G5 / Dl500 .I 31 052199098 TOX-120, RBL04199-25Oppb-CCllI 4/19/99 32 052199099 33 052199100 34 052199101 052199102 1 :: 052199103 37 052199104 38 052199105 1 39 052199106 40 052199107 41 052199108 MeOH Blank TOX-120 69M 6 5 / Dl500 TOX-12070M 0 5 I Dl500 TOX-120 71M G5/ Dl500 TOX-120 72M 6 5 / D1:500 Tox-120, RBL04199-25Oppb-CC,l 419/99 MeOH Blank TOX-210 73M 65 I D1:250 TOX-12074M 05 I Dl250 TOX-12075M 6 5 I Dl250 42 052199109 TOX-120, RBL04199-250ppb-CC1114119/99 . 052199110 MeOHBlank I : 45 052199111 TOX-12077M 6 5 IDl250 0521991-12 TOX-12078M G5I Dl250 46 052199113 TOX-120 79M 0 5 I D1:250 . : 47 052199114 TOX-120, RBLO4199-250ppb-CClI 419/99 48 052199115 49 052199116 50 052199117 MeOH Blank TOX-120, RBL04199-H2OBlk-l , 4/19/99 TOX-120, RBLO4199-LBlk-l,4/19/99 51 052199118 TOX-120, RBL04199-5PPb-1,4/19/99 i i 52 052199119 TOX-120, RBL04199-1Oppb-1,4/19/99 53 052199120 TOX-120,RBL04199-25Ppb-1 4/19/99 54 052199121 Tox-120, RBL04199-5Oppb-l,4/19/99 1 55 052199122 TOX-120, RBL04199-1OOppb1,4/19/99 i i 56 052199123 57 052169124 TOX-120, RBL04199-25OpPb-1,4A 9/99 TOX-120, RBL04199-500ppb-1,4/19/99 58 052199125 Tok-120, RBL04199-75Oppb-l,4l19/99 59 052199126 Tox-120, RBL04199-1000ppb-1,4/19/99 BACK TO MAIN MS File TELI-PF2 TEU-PF2 TELI-PF2 TELI-PF2 TEU-PF2 ELI-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PM TEU-PF2 TELI-PF2 TELkPF2 TEU-PF2 TEU-PF2 TEU-PF2 TELI-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PFP TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TELI-PF2 TELI-PF2 TELI-PF2 TEU-PF2 TEU-PF2 TELkPF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-Pf2 TEU-PF2 TW-PF2 TW-PF2 ' TEU-PF2 TEU-PF2 TEU-PF2 TW-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TEU-PF2 TELI-PF2 TEU-PF2 Inlet File Bottlle dume sample^ 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2. 9min-2 9min-2 Smin-2 9rnin-2 9min-2 9min-2 9rnin-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 91 91 79 80 81 82 83 84 85 86 87 88 89 92 57 58 59 60 . 90 96 61 62 63 64 99 92 65 66 67 68 100 93 :69 70 71 72 90 94 73 74 75 100 95 76 TI 78 90 96 79 80 81 82 83 84 85 86 87 88 89 10 Analyte 10 AnaMe 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte '10 Analyte 10 Analyte 10 halyte 10 QC 10 Analyte 10 Analyte 1.0 Analyte 10 Malvte OI Ana&te 10 Qc 10 Anabte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte i o Analyte 10 Standar 10 Standar 10 Standar 10 Standai 10 Standa- 10 Standa 10 Standa 10 Standa 10 Standa 1 I 1 I I 1 1 ~~ 801- D O 1 4 ( ) 8 0.00 ~ t & o o I l yP(ofamu~(=s?-) . rat- chumml De1.y ( See. 1 I span ( WtoM 1 I StartTL.(XiIm)r mdTL.(XhSlr. mt. I chnrul Paraat D m w h t u 0.03 0.00 0.00 9.00 1 -11 (Sees) Coll 1 399.00 99.00 0.25 45 2 39Y.00 130.00 0.25 45 1 1 I i I, 1 1 I I I (ev) COZU ( V 1 SO so ... BACK TO MAIN FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Analyst: k'h FACTStudyNumber 773x /20 I Matrix 1 ripoisb Dilutions? 3 3 1' I L 6 I ! f 1 11 Anstybr,3: 1 / / 1 I I i I .. BACK TO MAIN f 1 io osaimon 11 osaiwo71 i a osaissms 13 051199010 14 051199011 3 1 i o osaigson 31 osaiwom 31 o s a w o s s 31 OS1199100 36 osaissioi iio s a i m o ~ 31 051191101 u osaimo9 43 osaimio 44 os2191111 45 051199111 I i 3 I I 4119199 4119199 ._ ... ..- ._ 111.00 4 . u 5 a71.00 4 . ~ 5 SSt.00 133.00 1111.00 4.145 4.UY 4.145 m 1173 i 163.11 4 U 1 5 1 961.94 bbX -U 271.00 4.161 n1.00 4.168 a7a.00 4.84s 4.84s 4.us 4.84s 4.861 915 1 912 1 775 1 sas 1 361 1 271.00 4.us 4.145 4.14s 4.14s 4.161 146 1 150 1 600 1 1059 1 451 1 271.00 4.14s 4.14s 4.851 4.161 4.14s 4.14s 4.04s 271.00 4.161 .' SA 11.10 ai.ao 55.to 111.00 nl.w s~ st.00 133.00 1111.00 4.161 4.14s 4.u5 4.14s 4.14s ~4..-..u.s~ 4.145 4.84s a03 in 41 13 UI 191 ~749 . 1134 I 150.55 m I 13.94 m 1 31.36 IOI 1 61.93 MI 1 I iao1a8..suomm 1 . ssa.14 m 1 135.44 bb I a6 13 11 -a . 4 -1 0 h 1 1 1 2 1 4 5 7 1 3 N.Qw U d hx-iao. UAI '1 n aa a9 30 1.1. 3a 33 osaimoi 052199102 052199103 4I 49 50 54 55 s_ s . 57 5I 5Y hx-no' Gii hx-120: IBL04199-50&-1; 4IU19Y .75opp&l. 4119IY9 .1OOoppbl. 411I199 .La- m? 5.11s .5.116 1.00 5..11.k ~ 1.00 5.116 1-00 5.111 1.00 5.116 1.00 5.111 1.00 5.092 1-~.00~~ s.091 1.00 S.OY7 1.00 5.116 sou sa1 ~ 6020 5510 5195 s447 50U 4819 4ma 5090 5m 1.00 5.116 5.116 5.116 5.116 3Y0 3Y7 373 353 4751 5.lM 5.116 5.116 5.U6 1.00 5.116 376 366 370 331 5119 rn i 0.11 ut I 0.~5 m I- 1-..0.1. h-b 1 0.99 bb i 0.93 1 0.m bb 1 0.91 bb 1 0 . U bb I 0.17 bb 1 0.91 bb 1 i.oa m 1 0.07 bb 1 0.07 bb 1 0.07 bb 1 0.06 bb 1 0.15 bb -1 0.07 bb 1 0.07 bb 1 0.07 bb I 0.06 bb 1 0.93 bb zon -9 -5 I -1 -7 -2 -9 -14 43 -9 a -I3 43 -93 -94 -u -93 33 -93 -94 -7 1.00 5.116 -23 1.00 5 . l l k 4172 1 om'm -l3 1.00 5.116 414k 1 0.74 bb -2 k 1.00 1.00 1.00 1.00 1.00 1.00 1 .oo 1.00 1--.0..0 1.00 5.116 5.llk 5:116 5.116 5.116 5.116 5.116 5.11k 5.111 5.116 5.116 5.092 4 1 M 1 0.87 bb -13 5301 1 0.Y5 bU -5 5294 1 0.95 bb -5 6498 1 1.16 bb lk i 5903 I ' 1.06 bb k 5620 i . o i bb 1 kloa 1 1.09 bb 9 579a 1 1.04 ta 4 5419 1 0.99 bb -a 57aa I 1.02 ta 2 5414 I 0.97 bb -3 (305 1 1.13 bb 13 BACK TO MAIN i.oooooo. mvbla 1.000000 1.000 <. 4.840, o.ooo, m u 0.595 Diubld 3 a . b 1100.00.0oo 0.00 10.00 DimAbld 40.00 1.50. Dinabhd 0.00. D I H b l d 1.00. D h r b l d .. BACK TO MAIN .. ...... .- 1.000000. D i a d 1.000000 a.ooo %la% nAbld 0.000. D i a d 0.410 Yuut IUl.tIw AbmOlUt. IUl.tIw Abmolut. h.I*t: h.i*tl .ryt -1 ao.oo 1oo.oQ 0.00 10.00 Isrbld 40.00 0.00, D i d d 1.00. m a d 80.00. Ibrbld .. BACK TO MAIN .. . Eo. CUTIE "efofmipcioeunntd |ofnDaemtee:rmPiEnaHtSion: 0.99834 [Resbproantsieontycpuer:vEex:t1er.n3a5l89S9td*, xAr+e-a138506 (Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None 151e3 ]| L : BACK TO MAIN. 5 i ` 13 i 256% | | 1 x. x -% v Il Lox | 266 1 00 - 2000 4000 ~: : x -. z 600.0 8000 10000 5Conc Response Factor:5585.55 Response type: External Std,Area Curve type: FW 6.5Oe3 Response BACK TO MAIN X X 16. I- %Con X :B .*!. . . x X X :-\ X X X X X I I I 1 I I I I I. 1 X X 1 P -13.7 I I I I I I I I Conc 0.0 0.1 0!2. 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.o BACK TO MAIN 1 AMELIA062498: FACT Equipment Log 99016 Quattro 11, #6448E PAGE DATE: 05/25/99 . ANALYST hoj, sah STUDY NUMBER Tox-120,T0x-026 MATRIX: rat liver, monkey sera - DESCRIPTION dils. FACT Method: ets-M-5.0 Injection volume,lO S Cycle time: 9 min. Electrode: Z-Spray Mobile Phase: (A)2.0'mM NH4CH3CO2 3 TN-A- OT stndrd d e (A) 99012-10-7 drb Prepared(datdby): (A) 5/21/99 ias (B) MeoH @) TN-A-2540 . . . .. (B) 5/18/99 . Expires: (A) 11/99 (B) 11/99 Storcatroomtemp? or N other: Gudrd column: None . Analytical column: keystone betasil . Size:2x5Omm Type:c18 Spheresize:5 urn Part number: 055-701-2 . Lot rmmber: P7J20 Serial number: 038126D 1 Analytical parameters: Drying gas (I& 300 ):ESI nebulizinggas (uhr) 15 Sheath gas (Lmt.) 0 LC Flowrate:300 Uymin. Back ptessure: 70 bar 3 HPLC solvent gradient:9dn-2 Time (min.) %B 1) 0.00 40 2) 1.0 40 3 4.5 . 95 5) 6.5 95 6) 7 40 . 7) .9 end Tuning Method Report Method: Printad : C:\MASSLYNX\FLATS2.PRO\ACQUDB\PFOS Tue May 25 15:22:53 1999 BACK TO MAIN ' Page 1 SOURCE (.ESP- ) Capillary Cone Extractor RF Lens Source Block Desolvation Temp. Temp. Set 3.00 15 5 0.2 150 250 Rdbk 2.98 -15 -5 150 251 Pressures Analyser Vacuum Gas Cell Rdbk 1.6e-4 4. Se-3 MS1 LM Resolution HM Resolution Ion Energy . Ion Energy .Ramp Lens 6 Multiplier Set Rdbk 15.0 15.0 2.0? 0.0 . 5 7 650 :\ Ms2 LM Resolution HM Resolution Collision Ion Energy Ion Energk Ramp Lens 8 Lens 9 Multiplier S e t Rdbk 15.0 15.0 . 30 46 2.0. 0.0 40 40 . o 48 650 --ai- - Masslynx Sample Llst Sample Ust C:\MASSLYNX\rats2.PRO\SampleDB\O52599a.SPL Printed: Tue May 25 15:16:14 1999 File Name File Text 1 052599001 2 052599002 3 052599003 I 4 052599004 5 052599005 6 052599006 7 052599007 1 8 052599008 9 052599009 methanol blank methanol blank tox120, rbl04199-wblk-1 tox120, rb1041941bIk-1 tox120, rbi041945ppb-1 tox120, rb104194lOppb-1 tox120, rb10419425ppb-1 toxl20, rb104199-5oppbl toxl20, rbl04199-1ooppb-1 * 3 10 052599010 11 052599011 12 052599012 13 052599013 tox120, rb104199-25Oppb-1 toxl20, rb104199-500ppb-t toxl20, rb104194750ppb-1 tox120, rb1041941oooppb-1 1 14 052599014 15 052599015 10 052599016 17 052599017 18 052599018 1 19 052599019 20 052599020 21 052599021 22 052599022 methanol blank toxl20, rtl, 3Mgl-250 ppbms, 4/19/99 to~120N, ,3Mgl-250 ppb-wd, 4/19/99 to~120r,tl, llOF91-250 ppb-msd, 2/22/99 bx120, rtl, 11OFgl-250 ppb-msd, 2/22/99 tox120, rtl, 106Fgl-250 ppbms, 2/22/99 tox120, rtl, 106Fgl-250 ppb-msd, 2/22/99 toxl20, rb104199-250ppb-cc-4 methanol blank 1 23 052599023 24 052599024 25 052599025 20 052599026 tox120, rtl, 81M-g6,4/19/99, dlOOO tox120, N,82M-g6,4/19/99, d1000 tox120, rtl, 83Mg6,4/19/99, d1000 toxl20, N,84M46,4/19/99, dlOOO 27 052599027 tox120, rtl, 85Mg6,4/19/99, dlOOO 1 28 052599028 tox120, rb104199-25oppb-co-1 . ! 29 052599029 methanol blank 30 052599030 tox120, rtl, 86M-g6, 4/19/99, dlOOO 31 052599031 tox120, rtl, 87M-g6.4/19/99, dl000 32 052599032 I 1 33 052599033 . 3 4 052599034 35 052599035 30 052599036 37 052599037 1 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 3 43 052599043 44 052599044 . 45 052599045 46 052599046 I 47 052599047 48 052599048 49 052599049 50 I * 51 052599050 052599051 \Q 53 54 052599052 052599053 052599054 55 052599055 J 56 052599058 57 052599057 58 052599058 59 052599059 1 60 052599060 toxl20, rtl, 88M-g6,4/19/99, d l000 toxl20, rtl, 89M-g6, 4/19/99. dl000 toxl20, rtl, 90M96,4/19/99, dlOOO tox120, rb104199-25Oppkc-6 methand blank tox120, rtl, 91M-g6,4/19/99, dl000 tox120, rtl, 93Mg6,4119/99, d l000 tox120, rtl. WMg6.4/19/99,d l000 toxl20, rtl, 95M-g6,4/19/99, d l000 tox120, rb104199-250ppb-c~-3 methanol blank tox120, rtl, 17M-g2,4/19/99, d25 tox120, rtl, 18Mg2,4/19/99, d25 tox120, rtl, 19M-g2,4/19/99, d25 tox120, rtl, 20M-g2,4/19/99, d25 tox120, rtl, 21M-g2,4/19/99, d25 tox120, m, 22~-g2,41img,a5 tox120, rb104199-25Oppb-c~-11 methanol blank toxl20, rtl, 17Mg2,4/19/99 toxl20. rtl, 18Mg2,4/19/99 toxi20, m, ig ~ - g 2 , 4 / i m g to~120,rtl, 20~g2,4/19/99 tox120, rtl, 21M92,4/19/99 bx120, rtl, 22M-g2,4/19/99 tox120, rb104199-250ppb-c~-2 methanol blank toxl20, rtl, 41M-g3,4/19/99 tox120, h104199-wblk-1 I i i i BACK TO MAIN Page 1 YS File Inlet File 9MIN-PFOS-POW-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-6 13 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MiN-PFOS-POAA-513-613 9MIN-PFOS-POW-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POW-513-613 9MIN-PFOS-POAA-513-613 9min-2 9min-2 9min-2 9min-2 Qmin-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9MIN-PFOS-POW-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-51-13 9min-2 9MIN-PFOS-POW-513-613 9min-2 . 9MIN-PFOS-POAA-513-613 9min-2 QMIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POW-513-613 9min-2 OMIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POW-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOSPOAA-513-613 9min-2 9MIN-PFOS-POW-513-613 9min-2 9MlN-PFOS-POAA-513-613 9mln-2 9MIN-PFOS-POW-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOSPOAA-513-613 9min-2 . 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9mln-2 9MIN-PFOS-POW-513-613 9min-2' 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 . 9MIN-PFOS-POAA-513-613 9min-2 SMIN-PFOS-POAA-51-13 Qrnin-2 9MIN-PFOS-POW-513-613 9min-2 9MIN-PFOS-POM-513-613 9min-2 9MIN-PFOS-POAA-513-613 9mln-2 +a, 9MIN-PFOS-POAA-513-613 9mln-2 QMIN-PFOSPOAAS13-613 9min-2 9MIN-PFOSPOAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9min-2 9MIN-PFOS-POAA-513-613 9mln-2 9MIN-PFOS-POAASl3-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 QMtN-PFOSPOAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOSPOAA-513-613 9MIN-PFOSPOAA-SI3-613 9MIN-PFOS-POW-513-613 9MlN-PFOS-POW-SI3-613 9MIN-PFOS-POAA-5I3-GI 3 9MIN-PFOS-POW-513-613 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 Qmin-2 9min-2 9min-2 9min-2 9min-2 9min-2 9MIN-PFOS-POAA-513-613 9min-2 - ! Masslynx Sample Llst Sample U s t C\MASSLYNX\rats2.PRO\SampleDB\052599a.SPL Printed: Tue May 25 15:16:14 1999 File Name 61 052599061 62 052599062 63 052599063 64 . 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599009 70 052599070 1 File Text tox120, rblOrllSslblk-1 tox120, rbl041994ppb-1 toxl20, rb1041941Oppbl toxl20, rb104199-25ppbl tox120, rbl04199-5oppb-1 toxi20,rb104199-1ooppb-1 toxl20, rblO4199-25Oppb-1 tox120, rbio4199-5Ooppb-1 . t0~120,rbiO419475oppb-1 * toxl20, rb104199-1oooppb-1 .... . . ., BACK TO MAIN Page 2 MS File QMIKPFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MINPFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOSPOA4-51-13 9MIN-PFOS-POW-513-613 QMIN-PFOS-POW-513-613 OMIN-PFOS-POW-513613 9MIN-PFOS-POW-513-613 Inlet File 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 9min-2 . .. ' . . 1 1 1 1 i .. I i ! i L B"! . ldmt Ddw ( mina ) : 0.00 lUnotiOnr1 )awoflIru.Nr(zsP-) Inter Channel &lay ( S e a 1 t Spam ( Dalton. I : StUt T h ( WM 1 : tnd T f u I M h 8 I : Repbata : channel Parant Daughter 0.03 0.00 0.00 9.00 1 -11 [ s e a l C o l l Energy (eV1 Cone ( V I 1 413.00 169.00 0.30 30 1s NnatiMrl ##dl#uRZrfUP) I n t e r Channel Delay [ S e u 1 : . Span ( Dalton. I I S t a r t Tima ( Mina J : EadTima ('Mirm I : napaat. : Ch.na.1 Parant , Daugbtu 0.03 0.00 0.00 9.00 1 mall ( s ~ u )-11 ] . 1 513.00 169.00 0.90 30 Energy (ev) Con. ( V 1 15 Nnottoa: 3 ##oii#..Afr (UP) 1 I n t e r Chumel Dalay ( S e u 1 : S S p t u a r r t I~Dailtom(n.m1 :1 : h d T i u I WM I : -peat8 I 1 - e a r a t Dauqhtu 0.03 0.00 0.00 9.00 1 mal1 (sacs) -11 1 613.00 169.00 0.90 30 mmrG (av) ana t v 15 Nnotionr4 rnoilIru.~(mP-) Inter channal h l a y ( smu 1 : Spur ( Dalton. 1 I Start Tima ( 1 : CndnU(MInm1: Repeat. : ChuUral Parant Daughter 0.03 0.00 0.00 9.00 1 Dwall ( S e a l Coll Energy lev1 CoM (+V 1 1 499.00 99.04 0.3Q 45 60 I\mctLon:S W#ofl#..P.irIEaP-) I n t u Channel Oalay ( Saw ) : 0.03 SPUI ( Dalton. 1 : 0.00 0.00 End Time Mins 1 : 9.00 Rapeat. : 1 Channel Parant Diuqhter -11 (Saul Coll Energy (eV1 1 427.00 00.00 0.30 40 COM 1 V 1 60 BACK TO MAIN . .. . .. . . .. FACT: DATA REVIEW SUMiMARY FORM BACK TO MAIN --. Date: .. Analyst: &J FACT Study Number Maltix r iPoints i % - 1 I 1' I i I .. I I 1 1 1 1 1 I f I . i I I BACK TO MAIN ho. 1 Printad: W a d m y 26 00:20:32 1999 1 3 1 7' ! j ! I I f 1 .I i 1 / .*I ,line 1 052599001 2 052599002 E22: 3 OS2599003 4 052599004 5 052599005 6 052599006 1 OS2599007 a oszsssooa 9 052599009 10,052599010 11 052599011 12 052599012 13 052599013 - _ 14 052599014 w 15 052599015 ., 16 052599016 1' 1,44 17 052599017 ., - 11 os259soia ., 19 052599019 ., 20 052599020 21 052599021 22 052599022 23 052599023 24 os5sssoi4 25 052599025 26 052599026 27 osisssoi~ . ze 052sss02a 29 052599029 222: 30 052599030 . 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 31 052sss03a 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 osis99044 s 2 : 45 052599045 46 052599046 47 052599047 4) 052sss04a 49 052599049 50 052599050 51 052599051 52 052599052 ..' . 53 052599053 .54 052599054 &@.Lint Tut u-1 blank u t h u m l blu& tOX120, rbl04199-*1k-l toxl20, rbl04199-lblk-1 toxlZ0, rb104199-5ppb-1 toxl20, rblO4199-1Qppb-1 toxl20, rb104199-25ppb-1 tox120. rblO4199-SOD-1 tox120; rtI1.04199-10-*1 tox120, mI104199-25OpF&l toxl20, rtI104199-5Ooppb-1 tox120, mI1.04199-750ppb-l toxl20, rt . 0 4 1 9 9 - 1 0 0 ~ 1 Uthurol tI:11.u& toxl20, rt , m-1-250 ppbY, 4/19/99 . + b tox120, rt:1 34-91-250 m d , 4/19/99 toxlZO, rt ., 1101-91-250 ppb-mS#, 2/22/99 toatl20, rtA 1101-ql-250 ppb-yd, 2/22/99 toxl20, rt:1 106F-gl-250 ppby, 2/22/99 toxl20, rt:l 106P-91-250 m d , 2/22/99 toxlZO, rt11.04199-25oppb-cc-4 U t h n o l tI1 toX120, rtl, Ill4-qS. 4/19/99. dlOOO tox120, .rtl, (PI-96, 4/19/99, dl000 tox120. rtl. 13W-a6. 4/19/99. dlooo tOX120; rtl, OUI-qb; 4/19/99; dlOOO tOXl20, rtl, Os(-g6, 4/19/99, dl000 toxl20, rb104199-25Oppb-cc-1 methanol blank tox120, rtl, OQI-qb. 4/19/99, dl000 tOX120, rtl, om-q6, 4/19/99, dlOOO toxl20, rtl, Em-96, 4/19/99, dl000 tOX120, rtl, OW-g6, 4/19/99, dl000 tOXl20, rtl, 9OX-g6, 4/19/99. dlOOO toxl20, rb104199-25Oppb-ct-6 Uthanol blank tox120, rtl, SUI-qb, 4/19/99, dl000 tox120. rtl. 93W-gb. 4/19/99. d l o o o tOXl20, rtl, 94N-q6, 4/19/99, dlOOO rOX1201 rtl, 9%4-g6. 4/19/99, dlOOO -120, rb104199-25oppb-~3 ~8tlunolblank tox1 rtlb im-qz, 4/19/99, d25 tox1 toxl told rtl, law-q2. 4/19/99, d25 rtl. lSX-q2, 4119199, d25 rtl, ZOH-qZ, 4/19/99. d25 toxl rtl, ZlM-qZ, 4/19/99, d25 toxl rtl, 2PI-qZ. 4/19/99, d25 toxl rb104199-25Oppb-ccll Mth no1 blank tox1 0, rtl, im-qz, 4/19/99 tox1 0, rtl, la-92, 4/19/99 toxl 0. rtl, 1Si-qP. 4/19/99 toxl 0, rtl, 2OH-02, 4/19/99 zup. 8td CaM 12 Arulyta Aru Analyta Stanbrd standard standard Standard stand8rd S.56 11.10 27. 90 . 55.60 111.00 4.950 5.051 5.300 4.~5 4.979 4.901 4.924 23 111.15 m 14 -70.i7 bb 4 a.47 bb 52 4 6.69 bb -40 1 30.61 bb 10 11 16 S t a d 278.00 5.023 55 Standud 556.00 5.011 102. qt).tmd.rd a33.00 s . o a ' 133 Standard 1111.00 5.022 150 halyta halyta 4.996 40 5.033 44 mure myt. 4.997 5.005 40 . 31 5.000 39 5. 004 47 27a.00 5.028 45. Aaalyt. Anal*. Anal*. Analyta Qc 27a.00 5.004 45 256.64 bb -9 Anllyt. Anllyt. Atulvta 27a.00 5.236 5.042 Atulyta Arulyta Atulyte qc Anllyt. Aaalyt. Atulyt. Arulyta Arulyta Qc Arulyta -yt. Arulyta Analyt. Arulyt. . 27a.00 z7a .oo 5.055 5.042 5.060 5.046 5.023 5.024 5.025 5.026 5.026 5.030 5.024 5.020 4 9.54 bb 47 \ 266.91 bb -4. . 55 314.02 bb 13 36 26 56 336.91 bb \ I 55 35 50 44 430 2_ 61.5_ .70_ . b.b 4 ~~ 570 3410.25 bb 523 3130.06 bb 576 3-4.4.6...0.3. bb ~~ 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052593066 69 052599069 70 052599070 toxl tox1 toxl matt toxl toxl t0Xl tOx1 toxl toxl tox1 t0Xl tOX1 toxl toxl toXl 0, rtl, 2W-q2, 4/19/99 yl0, rti. zm-qz. 4/19/99 0, rb104199-25Oppb-cc2 blank 0, rtl, 4uI-q3. 4/19/99 '0, rb10419Wlk-1 0, rb104199-lblk-1 '0. rb104199-5ppb-1 '0. rb104199-1oPp$-l '0. rb104199-25ppb-1 :O, rblO4199-5Oppb-1 0, rbl04199-10OpF&l '0. rblO4199-25QPptil !O, rblO4199-SOOppb-1 !O, rb104199-75Oppb-1 '0. rb104199-100QPptil Atulyta Analyte a Arulyta Anal*. Amlyta blyt. Standard Standard SMud SMrd Standard Standard sturd.rd Standard standard z7a.00 5.56 11.10 27.ao 55.60 111.00 271.00 556.00 133.00 1111.00 1.019 5.024 4.994 5.024 5. 030 .&\ 5.044 4.7~ 5.045 5.027 5.020 5.024 4.972 539 3224.10 bb 442 2640.17 bb 39 211.34 bb -22 317 16 9 36.60 m u .32 20 103. ai bbx 17 19 97.55 bbx -12 46 264.19 mK -S 79 462.30 mK -17 as 497.64 bbx -40 115 cl6.00 m a -39 t . BACK TO MAIN J 3 '1 1 coqparad 2: N513 4' * ImYI 1 O! ii!?i9I9001 2 O! ii!?i919002 3 O! ilISI919003 4 0silISI9'9004 5 0:il13I919005 6 O! i i15I919006 7 02iZIS1919007 8 03i2I5 19008 9 osilIS '9009 10 osi2:5 '9010 11 05il15 '9011 12 osilI5 19012 13 osilIS '9013 14 osilI5 9014 15 05i l!S '9015 16 0:il!S 19016 17 OSilIS 19017 18 O! ilI5 '9018 19 03il!SI919019 20 01i215t989020 21 05il15I919021 2~2- O! il!S 19022 23 O! i2!S 19023 24 O! i2!5 19024 25 O! il 19025 26 O! ii 19026 27 O! ii I9027 28 O! il 19028 29 0: ii 19029 30 0:il I9030 31 01il!SI9I9031 32 O! i i!aI9I9032 33 O! i2!SI919033 34 0:i i!519I9034 35 O! i i!SI919035 36 O! iZ!S 19036 31 0: !S I9037 38 0: !? 19038 39 O! !5 I9039 40 O! !S 19040 41 O! !S 19041 42 05 15I919042 431 O! !?i919043 44 45, O! !!i9I9044 O! ii!?i9I9045 ' 46; O! ii!?i9I9046 47 O! ii!II9I9047 401 O! ii!? i9I9048 491 O! ii!!i919049 501 O! ii!?i9I9050 51 O! i i!EI9I9051 52 O! ia!SI919052 53, 0:ia!E,919053 54 O! !?i9I9054 55, O! !Si919055 56~ 0: !II9I9056 57 0: I919057. 58 0: I919058 591 0: 1919059 601 O! I919060 61 O! I919061 62 O! I919062 . 63 si O! O! 65, O! 66, O! 19 19063 I9I9064 I 919065 I 919066 67 O! ii!Si9I9067 68 O! ii!Ei9I9068 691 O! ii!?i919064 7c1 O!5:t!SJ19070 -diBt M Mthanol blank Mth-1 blank toxl20, rblO119941k-1 tOxl20, rb104199-lblk-1 toxl20, rblO4199-5ppb-1 toxl20, rb104199-Ioppb-1 tOXl20, rb104199-25-1 tW120, rb104199-5Oppb-1 toxl2O. rblO4199-10-1 t 0 X l !O, rb104199-250&-1 tOX1 !O, rb104199-5OOppb-1 toxl !OI rb104199-75Oppb-1 toxl11!O, rb104199-1OOOppb-1 UttUmol blank cox112 toxli!O, rtl, M-q1-250 ppb-ud, /19/99 toxli!O, rtl, IlOF-gl-250 ppb-df 2/22/99 toXli!O, rtl, 1lOF-ql-250 p p b y d , 2/22/99 * toxli!O, rtl, 106F-91-250 ppb-nr, 2/22/99 toxl1!O, rtl, 106F-91-250 pptiud, 2/22/99 tox1i!0, rb104199-25Oppb-ce-4 mtIu-1 blulk toxliIO, rtl, 8lM-q6, 4/19/99, dl000 toxli10. rtl. 8%-US. 4/19/99. dl000 tWCl20; rtl; 834-;6; 4/19/99; dl000 tOXl20. rtl, 84U-qS. 4/19/99, dl000 toX120, rtl, 8%-q6, 4/19/99, dlOOO tOXl20, r b 1 0 4 1 9 9 - 2 S ~ b - c ~ l utlunol blank tOXlZO, rtl, 86n-06, 4/19/99, dlOOO toxl20, rtl, ORI-q6, 4/19/99, dl000 ' toxl20, rtl, em-qs, 4/19/99, dlOO0 tOX120, rtl, 89X-96. 4/19/99, dl000 tox120, rtl,. 9PM-qbr 4/19/99, dl000 toxl20, rb104199-ZSCppb-cr6 wthanol blank tox120, rtl, 91X-q6, 4/19/99, 61000 tOXl20. rtl, 934-q6, 4/19/99, dlOOO tOXl20, rtl, OUI-qS, 4/19/99, dlOOO tOXl20, rtl, 9%-q6, 4/19/99, dlOOO tOXl20, rb104199-Z5Oppb-cc-3 lathan01 blank toxlzo, rtl, im-q2, 4/19/99, ~1.25 tox120, rtl, 18n-q2, 4/19/99, d25 tOX120, rtl, l%-q2, 4/19/99, d25 tOX120, rtl, 20Wq2r 1/19/99, d25 _ _ tOXl20, rtl, 2lM-q2, 4/19/99, d25 tOXl20, rtl, 22U-qZ2.4/19/99, d25 tOXl2O. rb104199-25O~~b-ce-11 rtb&l blank toxi20, rtl, 17n-q2, 4/19/99 tOXl20, rtl, 18n-gZ. 4/19/99 toXlZO, rtl, 19k4-qZr 4/19/99 tOXl20, rtl, 20n-q2, 4/19/99 tOXl20, rtl, 2111-92. 4/19/99 tOX120. rtl. 2pI-92. 4/19/99 tox120, rb104199-25oppb-cc-2 methanal blank toxl20, rtl, 4W-q3, 4/19/99 toxl20, rblO4199-ublk-1 tOXl20, rblO4199-lblk-1 tox120, rb104199-5ppb-1 toxl20, rb104199-loppb-1 tox120, rb104199-25ppb-1 tox120, rb104199-5Omb-1 tox120, rb104199-1OOppb-1 tox120, tblO4199-25Oppb-1 tox120, rb104199-5OOppb-1 tOXl20, rblO4199-75Oppb-1 tox120, rblO4199-100Oppb-1 std corm COM. nag. 8D.V 5.56 11-10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 278.00 5.471 5.41)) 5.464 5.488 5.455 5.469 5.465 5.750 5.462, 5.475 5.463 5.476 5.461 5.494 5.446 5 12.91 bb -54 13 72.45 m 30 21 130.40 bb 17 46 316.68 nn i4 77 540.85 bb -3 110 788.13 m -5 129 922.50 MU! -17 4 0.98 34 226.51 39 ~ 6 2 . w ze 180.25 26 162.57 41 z n . 3 0 42 283.04 36 240.93 5.470 .. 5 11.89 bb pc AMlyt. . 278.00 5.470 37 245.31 l@l -12 An.lyt. 2% Anal*. -1yt. Qc 278.00 5.486 40 267.80 bb -4 Llyt. Analyt. -1yt. An8lyt. An.lyt. pc 1wlyt* An.lytE AMlyt. Analyt. 27e.00 5.476 5.460 5.502 5.513 34 228.46 bb -18 1 9 40 68 bb 6 16157 lQ4 13 I I Analyta 5.489 16 Analyt. 5,511 21 Myt. Qc Anrlyt. Analyt. AMlyt. AZUlyt. 270.00 5.494 5.515 5.492 5.471 5.490 14 32 155 1113.48 lQ4 ,<? 120 861.12 bb 138 987.81 IN Analyt. 5.471 157 1131.41 .bb AMlyt. Anrlyt. EZz Qc -1yt. 278.00 51486 5.4l)a 1.485 5.468 161 1363.29 bb 104 741.94 bb 33 216.29 W -22 91 643.40 bb Analyt. Sund.rd stuId4rd Stlndud Stuldud 5.56 11.10 27.80 55.60 5.465 :\ 2 5.510 .9 5.501 11 0.00 bbX 37.86 lMC 52.34 bbX -100 36 -6 atur&ud 111.00 5.488 22 139.09 bbX 25 standard standard 278. 00 5.470 556.00 5.464 31 223.39 bbX -20 61 427.20 bbX -23 standard standard 833.00 5.465 1111.00 5.487 71 496.09 bbX -40 74 520.73 bbx -53 BACK TO MAIN lupla Limt: u.t r o d L C i d : mthod: L u t -rid: Job cod.: 8:\J'ACT-l.pRo~0Dm\05149Sa w #y 17 u:i4:20 199s 8:\~T-l.OAD\W.thD1I\OJl49Sa Ibo 11 14:23:= 1999 Rintdr Tue May 10 00:22:40 1999 t & m m d 2 : w163 #1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 7 051499067 e o~i49906e 9 051499069 Malimt Tut W&I Blank x.oU Blank , TOX-120, RBLO4199-8208lk-2, 4/19/99 ToX-120, RBLO4199-IBlk-2, 4/19/99 Tox-120, RBLO4199-5ppb-2, 4/19/99 TOX-120, RN.04199-l0ppb-2, 4/19/99 Tox-120, RBLO4199-25ppb-2, 4/19/99 Tox-120, RBLO4lSS-5-2, 4/19/99 Tox-120, RBL04199-1OOpPb-2, 4/19/99 Tox-120, RBLO4199-25-2, 4/19/99 ToX-l2U, RBLO4199-500ppb-2, 4/19/99 TOX-120, RBL04199-15opp&2, 4/19/99 . Tor-120, RBLO4199-1OOOppb-2, 4/19/99 x.08 Blank rox-izo 2 3 ~e./m:zs TOX-120 2uI Q / Dl:25 27 051499007 20 0514ssoae 29 051499009 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 0514ssose 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 40 051499100, 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 TOx-120-33N w / Dl:z5 lox-120 34M 63 I Dl:25 Tox-120 3511 W / D1:ZS TOX-120 3Ql 63 / D1:25 Tox-120 31N 63 / D1:25 TOX-120 3ON 03 I Dl:25 Tox-iio 381 w I ~ 1 : ~ s Tax-120 4On 63 / Dl:25 Tor-120, RBL04199-25oppb-cQ, 4/19/99 Me08 Blank mx-izo 2m GZ DI:LO' Tox-120 2% GZ / D1:lO ?OX-120 3On Q / D1:lO Tox-120 3U4 GZ / D1:lO Tox-120. RBL04199-25oppb-cC4, 4/19/99 Me08 Blank fox-120 4 a 63 / D1:lO T0x-120 4% 63 / =:lo Tox-120 45U 63 / D1:lO Tox-120 4SM 63 / D1:lO ?ox-120 4lN G3 I D1:lO TOX-120, RBL04199-25oppb-cC5, 1/19/99 MoOB Blank TOX-120. RBLO4199-BZOBlk-2, 4/19/99 TOX-120, RBLO4199-IBlk-2, 4/19/99 TOX-120. RBLO4199-5ppb-2, 4/19/99 TOX-120, RBLO4199-loppb-2, 4/19/99 TOX-120, RBLO4199-25ppb-2, 4/19/99 Tox-120, RBLO4199-5oppb-2, 4/19/99 TOX-12Q. RBL04199-10opPti2, 4/19/99 TOx-120, RBLO4199-25Oppb-2, 4/19/99 Tox-120, RBLO4199-500ppb-2, 4/19/99 TOX-120. RBLO4199-7Jogpb-2, 4/19/99 fox-120. R?L04199-1000ppb-2, 4/19/99 8td Con0 112 Aru 5.56 11.10 m.eo 55.60 111.00 27e.00 a 556.00 e33.00 1111.00 270.00 278.00 270.00 278.00 5.307 5.306 5.307 5.307 5.306 5.3011 5.308 1.308 5.315 5.307 5.306 5.307 5.300 5.306 5.307 5.307 5.306 5.307 5.306 5.307 5.306 1.56 ..LO .' - 0 0 e i.60 ii , 00 27 1.00 5s i.00 e3 i.00 111 .-00 613 C12.731 bbI 637 636.927 bb1 509 ' 509.031 bb1 473 472. 806 bbI 40 49 47 . 44 . 44 35 44 43 ' 48.0~- m4l msei 46.922 43.598 43.676 34.651 . m4l MU mu ma ma 44.313 MI 42.794 mu 1080 i079.6e4 bb1 1367 1367.145 bb1 1207 1206.705 bbI 1023 1022.573 bbI 66 66.125 m4l 03 83.0~6 bbI 50 50.209 Ma 63 63.197 1411 55 55.155 1411 . BACK TO MAIN 13 # Nuu W m l i i t ?ut 1 052599001 U r n n o 1 blank 2 052599002 UthAnOl blank 3 052599003 tOXl20, tb104199-!&lk-l 4 052599004 toxl20, rb104199-lblk-1 5 052599005 toxl20, rblO4199-5ppb-1 6 052599006 toxl20, rb104199-1Oppbl 7 052599007 tOXl20, tblO4199-25-1 8 0525990011 toxl20, rblO4199-5-1 9 052599009 toxl20. rb104199-LO--1 10 052599010 tOX1 0; rtI104199-25*1 11 052599011 12 052599012 . 13 052599013 14 052599014 15 052599015. 16 052599016 3 $\a 11 052599011 ia oszs99oia 19 052599019 20 052599020 21 052599021 22 052599022 ;. 23 052599023 ., 24 052599024 .. 25 052599025 26 052599026 27 052599021 * 28 0~2~99028 29 052599029 I ' 30 052599030 ..,, 36 052599036 31 052599031 ., 30 052599038 . 39 052599039 .. 40 052599040 41 052599041 tox1 0. rtI1.04199-50Oppb-l toxl 0. rtI104193-75Oppb-1 tox120; rb1104199-1OOOppb-1 U t bAno1 hilmk toxl20, rt:le 34-1-250 ppby, 4/19/99 toxl 0, tox1 0. rt:l, rt:1, m-q1-250 ppb-ild, llOF-ql-250 -/, 4/19/99 2/22/99 t0Xl 0, rt:18 110Fq1-250 pp&rs& 2/22/99 tox1 0. rt:I 106?-q1-250 ppb-ma, 2/22/99 toxl 0, rt:1 106Fql-250 ppbyd, 2/22/99 toxl 0. rtI1.04199-2SOppb-url Uth ~1 hI1. M k tOX1 0 , rt:1 rlX-qS, 4/19/99, dl000 tox1 0, rt:I am-qs, 4/19/99. a 0 0 0 tox120, rt:a 834-96, 4/19/99, dlOO0 tox12 0 , tor120. ., rt:1I, It :1 04X-qC. a%-q6, 4/19/99, dlOOO 4/19/99, dl000 tox120 , 11.04199-250ppb-cc-l Uthmno1 kI1.u* toxl20. rt:1I, 06U-96, 4/19/99, dl000 tow120. rt:1 0'RI-p6. 4l19199, dl000 toxl20, rt:1, 88X-q6, 4/19/99. dlOOO t0Xl 0 , rt::1 09n-q6. 4/19/99, dlOOO , tox1 0, rt 9W-q6, 4/19/99, dlOOO toxl 0. r bI1.0~199-25oppb-~c-6 math tox1 n0o,1rbtll.., au9klN-q6. 4/19/99, dlOOO tox1 0, rtl 9 3 W 6 , 4/19/99, dlOOO toxl 0, rtl 9(w-q6. 4/19/99, dl000 tox1 0. rtl SSW-US. 4/19/99. dlOOO \)cG 42 052599042 mOthurOl blmk 43 052599043 44 052599044 toxl2O. rtl, 1714-q2, 4/19/99. d ~ s tOX120, rtl, la-92, 4/19/99. dZ5 45 052599045 46 052599046 tOX120, rtl, l%-q2, 4/19/99, d25 tox120, rtl, ZOn-qZ, 4/19/99, d25 47 052599041 toxl20. rtl, ZlX-qZ, 1/19/99. d25 41 osz59904a 49 052599049 tOX120. rtl, 2ZU-q2, 4/19/99. d75 tOXl20, rb104199-250ppb--11 50 052599050 mothano1 blank J 51 052599051 52 052599052 53 052599053 54 oszs99osi 55 052599055 56 052593056 57 052599051 58 05~5990sa 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 61 052599061 68 os~~sso6a 69 052599069 70 052599010 tox120, rti, 17n-q2, 4/19/99 tox120, rtl, 18X-q2, 4/19/99 tOxl20, rtl, lW-g2, 4/19/99 tOX120. rtl. 2W-uZI.4/19/99 toX120, rtl; 2lX-a2; 4/19/99 t o X l 2 0 , rtl, 27.U-gZ. 4/19/99 toxl20, rb104199-25-sc-2 rthurol blank tox120, rtl, 4lX-q3, 4/19/99 toxl20, rblO4199-ublk-1 toXl20, rblO4199-lblk-1 toX120, rb104199-5ppb-1 tox120, rb104199-1-1 hXl20, rblO4199-2-1 tOXl20, rb104199-5Oppb-1 tOx120, rb104199-10Oppb-1 tox120, rb104199-25Oppb-1 toxl20, rb104199-5OOppb-1 toXl20, rblO4199-75-1 tox120, rb104199-1OOOppb-1 I I t A k l h 062498: wantiatad and printad by boj 5/26/99 i i i 8td Con0 R? 5.56 11.10 27.00 55.60 111.00 27a.00 556.00 a33.00 1111.00 . 1- 27a.00 5.795 5.791 5.765 5 . aoa 5.784 5.712 5.716 5.767 5.m 5.182 5.710 5.184 5.712 5.765 5.161 5.761 3 1.54 MW -12 . 5 8.66 la4 -22 14 32.23 bb 16 33 a2.54 bb 4s 53 131.12 bb 24 105 274.93 bb 101 266.49 W , -4 .. . 27a.00 5.192 112 295.72 MW 6 278.00 5.182 112 295.27 W 6 27a.00 5.795 5.181 100 262.77 W -5 s a.69 m 2ia.00 278.00 5.a~ 5.a1z 5.191 5.188 5.115 5.783 5.785 5.182 5.789 76 199.37 W -28 &> 39 9a 92 29 72186 81 210.54 MW ' . ..-24 27 66.29 bb 5.56 11.10 27.80 ~~ 55.60 111.00 z7a.00 556.00 833.00 1111.00 5.141 5.607 5.006 5.775 5.786 5.130 3.7a5 5.787 5.m :\ 191 . 13 11 41 el 150 204 252 11.56 i bbx i i m' bbX' 29.34 # b b x 23.51 bbx 103.06 lbbx 227.94 bbx 39). os1 bbx 542.63 I bbx 669.29 1 bbx 21,6 7 -6 . -I ,a ,7 -1 8 -1 18 -3 15 -4 0 BACK TO MAIN munary C-PoUnd -Y rOrl20. rtl -Port .1- Uat: C:~L'IIoRr8tm2.p a 0 ~ 9 B \ 0 5 2 5 9 9 a m o t modifid: W d #y 26 07:17:29 1999 Ibthod: C:-mrmW .Pm~thDB\052599a b o t modifid: Id May 26 07:18:17 1999 Job Cod.: Rintdr Id thy 26 08:20:32 1999 -4: PPOI 1052599001 2052599002 3 052599003 4 052599004 5 052599005 6 OS2599006 7 052599007 8 052599008 9 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599011 18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599021 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599031 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 052599056 57 052599057. 58 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 61 052599067 68 052599068 69 052599069 1 0 052599070 l)uptmliat h r t methanol blank Mth.nol blank toX120, rb104199-wblk-1 toxl20, rblO4199-lblk-1 toxl20, rb104199-5ppb-1 toxl20, rblO4199-1Oppb-1 toxl20, rblO4199-25ppb-1 tOXl20, rb104199-5Oppb-1 toxl20, rblO4199-1OOppb-1 toxl20, rb104199-25Oppb-1 - toxl20, rb104199-5OOppb-1 toxl20, rb104199-75Oppb-1 toxl20, rbl04199-100~-1 . methanol blank Jill toxl20, rtl, 3U-gl-250 ppby, 4/19/99 toxl20, rtl, 31(-g1-250 ppb-mad, 4/19/99 u,c tOXl20, rtl, 1lOF-91-250 ppb-M((, 2/22/99 toXl20, tOXl20, rtl, rtlo llOF-gl-250 l06F+-250 pm pb-mad,,22/2/22/29/999 toxl20, rtl, 106F-gl-250 ppbaad, 2/22/99 toX120, rblO4199-25Oppb-cc-4 Uthu101 blank toX120. rtl, 8lX-96, 4/19/99, dl000 toxl20. rtl. 8 Z N - d . 4/19/99. dlOOO tOXl20; rtl; 8&6; 4/19/99; dlooo toxl20, rtl, 84Jl-96, 4/19/99, dl000 tox120, rtl, 8.91-96. 4/19/99, dlOOO toxl20, rb104199-250ppb-ccl . mthanol blank tOXl20, rtl, 8a-96, 4/19/99, dl000 tOXl20, rtl, 8 R t - g 6 , 4/19/99, dlOOO tOXl20, r t L 88l4-g6, .4/19/99, dlooo tOXl20, rtl, 8%-g6, 4/19/99, dlOOO toxl20, rtl, Sm-g6, 4/19/99, dl000 toxl20, rb104199-25Oppb-cc-6 M u U n O l blank tox120. rtl, 9ln-g6, tox120, rtl, 931(-g6, toxl20, rtl, 94U-gC. toxl20, rtl. 9%-96, 4/19/99. dlooo 4/19/99, dlOOO 4/19/99. dlooo 4/19/99, dlOOO toxl20, rb104199-25Oppb-a-3 methanol blank . toxl20, rtl. 17M-gZ. 1/19/99, d25 toxl20. rtl, lm-gz, 4/19/99, d25 toxl20, rtl, LSM-gZ, 4/19/99, d25 tOX120, rtl, 2OH-g2, 4/19/99, d25 toxl20. rtl, 2U-g2, 4/19/99. 625 toxl20, rtl. zm-gz, 4/19/99, d25 toxl20, rb104199-25Oppb-crll methanol blank toxlzo, rtl, im-92, 4/19/99 toxl20. rtl, lm-92, 4/19/99 toxlZO, rtl, I%-gz, 4/19/99 tox120, rtl, 2an-92, 4/19/99 toxl20. rtl. .2ln-gz, 4/19/99 tox120, rtl, 2ZW-92, 4/19/99 tox120, rb104199-ZSOppb-ec2 methanol blank toxl20. rtl. 4W-a3. 4/19/99 tox120; a)io4ios4ik-i toxl20, rblO4199-lblk-1 tox120, rb104199-5-1 toxl20, rblO4199-1~&b-l tox120. rblO4199-25ppb-1 toxl20, rb104199-5-1 tOXl20, Eb104199-10-1 toxl20, rb104199-25Oppb-1 tox120. rblO4199-50oDub-1 tOX120; rblO4199-15~~-1 tox120, rb104199-100opptil 'Iypr Anal*. An81yt. Analyt. Arulyt. Standard Standard 'Standard Standard Standard Standard Standard Standard Standard -1s. AMlYt. 5.56 11.10 21.80 55.60 111.00 218.00 556.00 833.00 1111.00 - 211.00 pc . Analyt. AMlyt. halyt. Analyt. AMlyt. An.lyt. qc AMlyt. Anrlyt. Anrlyt. Anrlyt. Analyt. a AMlyt. Anrlyt. -1s. Analyt. Arulyt. AMlyt. Arulyt. pc -1s. Analyt. myt. &In.lyt. h.lyt. AMlyt. An.lyt. Qc Arulyt. Arulyt. Arulyt. Arulyt. Standard Stud~rd Standard Standard Standard Surd Standard stand8rd standard 218.00 278.00 211.00 S.56 11.10 21.80 55.60 111.00 278.00 556.00 833.00 1111.00 5.329 5.283 5.264 5.268 5.259 5.218 5.277 5.255 5.269 5.284 5.218 5.275 5.266 5.218 5.266 5.255 5.261 5.262 5.245 5.288 5.280 5.283 5.282 5.285 5.?84 5.285 5.260 5.274 5.300 5.294 5.280 Aru 13 34 42 43 92 129 195 445 8 61 1119 1321 496 504 627 666 516 506 440 5 361 693 494 600 669 405 679 634 566 466 470 4 04 9 389 311 370 367 365 6 1 16 * 5.282 279 5.282 81 5.284 64 5.281 66 5.281 54 5.300 59 5.303 52 5.275 339 5.284 61 :, 5.302 5.283 5.279 Si277 5.262 21 29 40 . 57 81 5.254 165 5.282 325 5.219 590 5,256 800 5.277 983 0.00 bb 0.00 bb 3.40 1QI -39 4.66 bb -58 41.91 bb 51 69.13 bb 25 120.06 bb 8 310.85 bb 12 628.49 bb 13 825.42 bb -1 984.54 rn -11 350.21 bb 356.00 450.18 480.05 365.31 351.63 307.74 0.00 246.90 500.27 489.72 bb 455.16 bb 403.35 bb 326.97 bb 330.46 bb 279.11 bb 1 0.00 la4 268.32 bb 209.11 bb 254.08 bb 251.43 bb O..OO bb 0.00 bb 0.00 bb 184.35 1pI 33.46 bb . 20.62 bb 22.28 bb 12.57 bb 16.68 bb 11.05 lm , 229.96 bb -34 2: . -17 18.46 bb 0.00 bb 0.00 1oQ( 1.17 14.92 bbX 37.99 bbX 91.67 lam 219.58 bbX 421.63 bbX 582.34 bbx 121.S4 bbX -100 -84 -46 -32 -12 -21 -24 -30 -35 uc Anulia 062498: w u ~ t i a t dand printod by b j 1/26/90 BACK TO MAIN # .ifam 1052599001 S.pl0li.t T u t utlunol blank i 2 052599002 rtlunol blank 3 052599003 tOXl2Or rb104199-wblk-1 I ! 4 052599004 tOX120, rb104199-lblk-1 5 052599005 toxl20, rb104199-5ppb-1 6 052599006 toxl20, ~104199-1Ogpbl 7 052599007 a OSZS~SOOS 9 052599009 I 10 052599010 I 11 052599011 1_2_ 012599012 toxl20, rb104199-Z5ppb-1 tox120, rbl04199-50ppb-1 toxl20, rblO4199-1OOppb-1 tox120, rb104199-25Oppb-1 toX120, Kb104199-50-1 toxl20, rblO4199-75oppbl 13 052599013 toXl20, tb104199-1000ppbl ' * 1 14 052599014 15 052599015 16 052599016 17 052599017 Uthurol blank tox120; rtl; 110?-~1-250A d ; 2/22/99 tox120, rtl, 106r-91-250 mb-mo, 2/22/99 1 tox120, -1, lOS?-q1-250 pirb-.sd, 2/22/99 tOXl20, rblO4lSS-25-4 rUthurol blank tOXl20, rtl, alN-q6, 4/19/99, dlOOO tOX120, rtl, 8M-q6, 4/19/99, dlOOO tOXl20, rtl, l3H-qb. 4/19/99, dlOOO toxizo. rti, am-qs, 4/19/99, a 0 0 0 1. _ _ rOxl201 rtl, 8SM-q6, 4/19/99, dlOO0 1 tOxl20. rb104199-25O~~bcc-1 methanol blank tOXl20, rtl, 1Q4-q6, 4/19/99, dlOOO toxizo, ;ti, am-ps, 4/19/99, dlOOO * tox120, rtl, 88n-q6, .4/19/99, dlOOO I toxizo, rtl, a%-96, 4/19/99, dlOOO toxl20, rtl, 90n-qb. 4/19/99, dlOO0 toxizo, rbia~i9~-zsoppb-cs-6 ! methanol blank toxl20, rtl. OlN-gtj, 4/19/99, dlooo 38 os2ssso~a tOXl20, rtl, 9M-g6, 4/19/9?, 61000 39 052599039 toxl20, rtl, SUI-qS, 4/19/99, dlOOO 40 052599040 tOX120, rtl, 9YI-qC. 4/19/99. dlOOO 4 1 052599041 tOXl20, rb104199-250ppb-~3 i 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 47 052599047 4a 052sss04a 49 052599049 50 052599050 51 052599051 MthurOl bl.nL . toxlzo. rtl, im-qz, 4/19/99, d25 toxl2O. rtl, 18n-ql. 4/19/99. d25 toxl2O. rtl, 1911-qZ. 4/19/99, d25 tOX12Or -1, 20n-2, 4/19/99, d25 toxlzo. rtl, Zu-qz, 4/19/99, d25 toxl20. rtl, 2M-q2, 4/19/99. d25 tOXl20, rb104199-25Qppb-c~l1 u u u n O l blank toxlzp, rtl, im-qz, 4/19/99 52 052599052 53 052599053 54 052599054 55 052599055 1 56 052599056 57 052599057. 51 052599051 59 052599059 60 052599060 61 052599061 I 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 1 68 052599061 6s 05iJss06s 70 052599070 toxl20. rtl, 18n-qZ. 4/19/99 tox120, rtl, LSM-qZ, 4/19/99 tox120. rtl, Zan-qZ, 4/19/99 tox120, rtl, ZlN-qZ, 4/19/99 t01120. rU. ULI-qZ, 4/19/99 toxl20, rb104199-25Oppb-cc2 mtburol blank tOrrl20, C t l , 4lN-q3, 4/19/99 toxl20, rb104199-ublk-1 toXl20. rblO4199-lblk-1 tox120; rblO4199-5ppb-1 tox120, rb104199-1oppb.l toal20, rb104199-2Sppb-1 tox120, rblO4199-5-1 toxl208 rblO4199-10-1 toxl20, rb104199-25-1 tOX120, rb104199-5OOppb-1 toxl20, rblO4199-75-1 tox120, rblO4199-1OOOppb-1 t 1 I 1 WP. Analyt. An.lyt. Anal*. Arulyt. Standard Standard Standard Standard. Standard Standard Standard Standard St.nd.rd AMlYt. S t d Con0 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 RT 5.042 s .022 4.999 5.004 5.022 4.993 4.992 4.993 4. sei 4.999 4.993 4.991 5.003 4.992 4.999 5.01) 4.999 4.99a Aru a26 1437 1653 1452 1463 1461 1233 1312 1146 1022 1071 1104 1260 197 . ZOO 219 235 1566 0.66 bb -34 1.16 bb 16 1.33 bb 33 1.17 bb 17 1.1) bb io 1.u bb ia 0.99 bb -1 1.06 bb 6 0.92 bb -8 0.82 bb -ia 0.16 bb -14 0.19 bb -11 1.01 bb 1 . 0.16 bb -14 0.16 bb -a4 0.1) bb -82 0.19 bb -ai 1.26 bb 26 . 1.00 5.?22 iiaz 0.05 bb -5 1.00 5.012 1232 0.99 bb -1 a An.lYt. AIUljrt. AMlytCl AMlyt. Anrlyta Arulyt. AIl.lyt. Qc Anal*. An.lyt. h.lyt. Anal*. Arulyt. h.lyt. Aarlyt. a Amlyt. Arul*. AIUlyt. Arulyta stmd.rd Standard Standud Standard StuldArd standud Standud Standard Stmdard 1.00 5.025 1129 5.010 5.029 5.041 5.018 5.019 5.046 1.00 5.020 43 . 47 44 43 29 52 952 5.020 5.024 5.010 4.998 5.013 4.994 1.00 5.012 4a1 529 446 570 446 512 1067 4.995 511 5.001 5.017 100 ioas 1.00 5.022 .sx 1431 1.00 5.016 1233 1.00 4.9~9 .1209 1.00 5.000 1262 1.00 4.992 1.00 4.997 1219 1055 1.00 4.991 1099 1.00 4.992 913 1.00 4.991 1054 0.91 bb. 0.03 bb 0.04 bb 0.04 bb 0.03 bb 0.02 bb 0.04 bb 0.77 bb 0.39 bb 0.43 bb 0.36 bb 0.46 bb 0.36 bb 0.41 bb , 0.16 bb 0.42 bb 0.64 bb 0.97 bb 1.1s bb 0.99 bb 0.97 bb 1.02 bb o.sa MJ o.as bb 0.118 0.79 bb 0.115 bb -9 -97 -96 -96 -97 -sa -96 -23 .=61 .'-57 -64 -54 -64 -59 .-I4 -sa -36 -13 15 -1 -3 2 -2 -1s -12 -21 -15 062491: q u u t t h t d and p r l n f d by a05 5/26/99 i i ournury -titoxlzo, rtl noport Qlibmu-: C : ~ t . 2 . R u \ ~ \ o m 9 ~ Laat rodighd: Id #u 26 07:SS:39 1999 ii P r i n W : Id #u 26 08:21:05 1999 2ompound 1 name: POAA 7 22oaelififriactieionnt ocfwDeet:e0rm.1i6n6at3i6o2n:*0x.9+920.34121271 esponsetype: External Std, Area ' b e type: Linear, Origin:Exclude, Weighting: lht, Axis trans: None I 18; 1 1 1 1 181' I I 0 'I 86.7 1 1 d .f 1 1- %Conc f f f x181 0 X i 1. . I , , , , #-, , , , , 2ob.o ,,,, ,,,, 40b.0 ,,,, ,, 60b.0 I"" e '-"I"" 80b.O BACK TO MAIN -- . a 181 Conc 1odo~ .o - . ~ l D BACK TO MAIN Z2aoleibffriactiieonnt ocufrDveet:e0r.m13in5a7t5io4n*:0x.9+839.94370400 tesponse type: External Std, Area 2urve type: Linear, Origin:Exclude, Weighting: l/x, Axis trans: None 154 181 . .. 181' 36.: "'1""1'"'1''', 81 X Y X ,,,, ,,,, I""I""I""I'"'I '- %Con( -53.6 I ("'I"" ,,,, ,,,) ,,, 20b.0 40b.0 60b.0 1odo.o . I I I 80b.I0. I I I , , . , , , , ,qConc 2ompound 3 name: M613 hXeifirfiactiieonntocfurDveet:e0r.m37in2a6t1io7n*: 0x.+9823.51571690 tesponse type: Extemal Std, Area W e type: Linear, Origin:Exclude, Weighting: l/x,Axis trans: None 416 BACK TO MAIN Response .. a >",I"" 216 ,,,, ,,,, ,,,, ) , , ) 1 , , , , 1""i""i""I t- %Conc X X X -72.2 , , , , I , , , , Ob 20b.0 , , , , I , , , , ,,,, 40b.0 60b.0 , , , , , , , , I , , , , , , , , I Conc 8Ob.O 1odo.o .. 1 1 ~.~ik.ti-: c : u m o ~ ~ . s m \ c ~ - ~ ~ \ o w s h L u t m o d i f i d : W d bhy 26 07:56:39 190, Rintd: Id Nay 26 0812l:OS 19S9 Compound 4 name: PYOS 1 CCoaelififriactieionnt ocufrDv-eti:on1:.31039 * x0+.93870.4129779 Response type: Extemal Std,Area Curve type: Linear, Origin:Exclude, Weighting: l/x, Axis trans: None 1 1.49e3 7 I 1 1' / 181 I I 0 I 50.2 I X I X X X I %Conc 181 181 I -181 181 181 I -84.1 I , , . ( ,,,, ,,,, 2ob.o 40b.0 I'.I. I 60b.0 80b.0 BACK TO MAIN X 181. ., ..a.' 181 ..,,,Cone 1odo.o BACK TO MAIN C.Zibrrtion1 G : ~ t 8 2 . R w \ c l m m D 8 \ ~ ~ k L u t mdifhd: Id m y 26 07:56:39 LSSS RinUd: Id May 26 OI:21r05 lSS9 )ompound5 name: IS Lesponse Factor 1242.19 Lesponse type: External Ski, Area ' M e type: RF 1 1.65e3 1 3 1 1' I i I I 33.1 1 1 1 1 I- %Con( f t I 1 I I I I I I X a 8 X X X I #x X I .x X X X J -203 Cow I 0: 1 012 0!3 014 0:s 0!6 0!7 0!8 019 IT0 t f I Method Report nethd file: Laat modified: C:~SLn#\rata2.~~e~\012599a.rQ, W a d May 26 07:10:17 19990 Compound: 11 POM Printmd: Wed Hay 26 O8:21:19 1999 Corpound N-I Internal Ref. : m n t i f y Tracer cone, of standard.: m [&MI TIC Caw. A ~ i Obc2Y48 Uaer RT Value: Uaer Peak h c t o r r Inteqratm Window Tactor: 1.000000. Disabled 1.000000 2.000 h a k location 1 RReeltaatnivtimonxTet.hnt( ia,lri ime~ : J:50..000100,, T b W i n d n r (m5naJ: 0.304 Peak Selection: N&mt Publd Diaablad Reamnee 3 Reaponam Type: Reaponae: Calibration Curvea . Dlterrul &*U 1 oolyndal Type: Point of Origin: T i t Weighting: hi. Tramformtion: Concantretion wm.U -1- ' m .. . - [Init88 Noise - Peak-to-peak up!itude: smooth 60 5.oothing: W i n d o w air. lacanal: Nunbar of mootha: ! - Smootbinq method: Baselinem ' Diaablmd 3 L . 2 . Join valley tbreaboldr Reduce peak t a i l i n g : Rehe baaellxu rulmum: 30.00 70.00 4.00 Peak Separation Draw v e r t i c a l thrmahold: Detect ahouldmra: Shoulder thrubold: I rnswnmm Threabold ' 10.00 Diaablod 10.00 i h h t i v e height: Abaolute height: nmlatiw arm.: Ab801Utm Um.2 , 50.00, Enabled 0.00, Diaab1.d , 3-00, -led 0.00. Disabled I 1- I I i f .. If' BACK TO MAIN _ L* Pagm 1 :3 '. .. i 1 1 1 1 I I I 1 1 1 1 1 f I i f Method bport nethod file: Laat modified: C:URSSLYNX\rat.~.~~~\052599a.db Ned MAY 26 01:lO:ll 19990 c-und: 2: MS13 Printed: colpound nuu: Internal Ref. : quurtify Tracer CMC. Of sturd.rdr: Wed May 26 Ol:21:19 1999 M513 tmnel TIC Cone. A AJiL Weer M Value: W a u Peak Tactor: Inteqrate Nindou hctorr 1.000000, Di8abl.d 1 .oooooo 2.000 h a k Location bt.ntion Time t . i n r ) r Relati- Retention T i m : T i U NbdOU (dM): f.551, publed 0.000, Diaab1.d 0.233 Peak solaction: Ymreat Reswnse Dt2qSP Calibration Curvea Polynomial zyp.: Point of Oriqin: rit Weighting: h i e Trumfozmatlon: Concentration unit.: - Noise Peak-to-peak up!itud.: - Smooth Smoothing: Window air. ( a u t u ) : Number of .nwtha: Smoothing method: Baselines Join valley threshold: -dum peak tailing: . -ire baaeline mixhum: Peak Separation Draw vmrtical threshold: Detect ahouldera: Shouldar threahold: Reswnae Threahold * Relati*. heiqht: Ab.olute heiqht: Relatiye arm.: Absolute arm.! 64 . Diaablad 3 2 30.00 70.00 4.00 10.00 Diaablad 10.00 ~. !~ io-0~ 0. .-lad 0.00, Disabled 3.00. -led 0.00, Di.ab1.d ! 1AC OSlab/qV BACK TO MAIN _-. Nethod Report Nethod file: Last modified: Cwund: Printrdt .. C:~SLrNx\rat82.mmthDB\052599a..Q, Wed Way 26 07:18:17 1 9 9 W 3: 1613 Wed W.y 26 0~:Zl:lS 1999 conpound Name: Internal Ref.: Quantify Trace: Cone.. of Standard#: W613 tNon.1 TIC conc. A U8er Iv Value: User Peak Factor: Integrate W i n d o w Factor: 1.000000, Di8abl.d 1.000000 . 2.000 Peak Location b Ratention T Z U b i n s ) : i . Relatiw Ratention T i u : T b Window ( a h a ) : Peak Selection: nesuonse 5.861, EMb1.d 0.000, Disabled 0.219 liurest I Resporm Type: Response: Calibration Cum8 Polynomial Typo: 1 Point of Origin: Fit Weighting: ani8 Trurarorution: Concentration 1' - Unitr: Noiae Peak-to-peak qlitude: swoth - Smoothing: 1 Window s i z e (scans): i Wruber of swotha: , Smoothinq method: 79 , Di8abl.d 3 2 Baselines . Join Valley threshold: Reduce peak tailing: b i 8 e baseline maximum: 30.00 70.00 4.00 Peak Separation Draw vertical thresholdr Detect shoulders: Shou1d.r threshold: ResWnse Threahold ' 10.00 Di8abl.d 10.00 hhtiV. heiqht: Absolute height: Relatiw .rea: Abao1ut. area! 50.00, BUb1.d 0.00. Di8abl.d 3.00, -lad 0.00, Di8abl.d .. Am.& OL2q91 . . . .. . .. (Ac m / 2 h BACK TO MAIN _..-* Page 3 rc: . Nathod Report Method f i l e : h a t modified: colrpouad: Printed: C:WAULna(\rata2.PRO\W.~\O52999..~ W a d b y 26 07:18:17 19990 4: P m s Wad b y 26 00:21:19 1999 compound Brama: 1 Intarnal Raf.: i Quantify Trace: I Cone. of Standards: INOII~I TIC Cone. A I user RI Value: User Peak Factor: Integrata Window h c t o r : Peak Location Ratention T i U (dN): Relatiw Retention T i u : 1.OOOOOO, Di.ab1.d 1.000000 2.000 S.281, EMb1.d 0.000, D i a a b l d I T i U W h d O W (dN): Paak Salaction: RaEPOM. 0.293 lienrut Ra8POlJU Typ.2 h8polu*: Calibration t u r v a m 1 POlynoltAl Typ.8 Point of Origin: ?'it Waightlagt M a Tr8MforUtion: 1 Concentration mit.8 'I' - Noiaa I Paak-to-p.ak - Soooth mplituda: ! I sloothing: ! window Sir. ( 8 C U l S ) I Number of nootha: sloothing r t h o d : . Prtenul Arau 72 . Diaeb1.d 3 '2 Baralinas 1 1 Join walley thrashold: Raduce peak t a i l l a g : Raise b a s a l h e maxim&: 30.00 70.00 4.00 Peak Sauaretion 1 Draw v e r t i c a l thrashold: I Detect shoulders: 1 Shouldar thranhold: Re8DOn#* Thhre8hold ? 10.00 Dlaab1.d 10.00 1 Ralatiw. height: aBOlUte haight: Ralatiwe am.: Abb.01uta an.: 50.00, -1.d 0.00, Disabld 3.00, pub1.d P 0.00, DtSab1.d 1 . I 1 I t BACK TO MAIN -- 7 paga I , Method kport Method file: Laat modiftad: Collpound: Printodx CJ UUSSL~\ratsI.PMU(.thDB\O52599...db Nod U y 26 07:1#:17 19990 5: IS Wed U y 26 Ol:Zl:l9 1999 - c m u n d Name: Internal Mf.: m t i r y Trace: Conc. 02 Standards: IS INOW TIC flird Uaer M Value: User Peak Tactor: Inteqrate Window Tactor: 1.000000, Disab1.d 1.000000 2.000 Peak loution Retention tiu Irina) x Relative Retantion T i m : T h WlndOW ( d M l : 5.029, -1.d 0.000. Diaabled 0.304 Peak Selection: Raawnse R..POMe -8 Reapomat Calibration Curves POlyaopZ.1 Type: Point of Origin: Fit Weighting: mi8 harufoartlon: Concentration Unit.: - Noise Peak-to-p.ak mplitude: - Smooth Smoothing: Windou a i m (scum)x Number of mootha: Smoothinq method: 83 .Diarbld 3 '2 Basellne~ Joln valley threshold: Raduce peak tailing: Raise bassline r u f n n r 30.00 70.00 4.00 Peak Seuarition Draw vertical threshold: Datect 8houldera: Shoulder threshold: . 10.00 Diaab1.d 10.00 Rerwnse Threshold Ralativa height: Abaolute height: Relative area: Ab6oluta aramx ,' . 50.00, Crmblad 0.00, Dirabld 3.00. B u b l d : 0.00. Diaab1.d BACK TO MAIN --* Page 5 A n Jifi OL2Y98 Ihc o f h L4! 9 .. .. *. . . - Masslynx Sample List Sample List C:WSSLYNX\rats2.PRO\SampleDB\060199c.SPL Printed: Tue Jun 01 16:19:18 1999 File Name 1 060199020 2 060199021 3 060199022 4 060199023 5 060199024 6 060199025 7 060199026 8 060199027 9 060199028 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 16 060199035 17 060199036 18 060199037 . . I 9 060199038 20 060199039 21 060199040 22 060199041 23 060199042 24 060199043 25 060199044 26 060199045 27 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 .060199054 36 060199055 37 060199056 38 060199051 39 060199058 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 - 4 6 060199065 47 060199066 40 060199067 49 060199068 . 5 0 060199069 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 56 060199075 57 OW199076 511 060199077 59 060199078 60 060199079 File Text methanol blank methanol blank tox120, rb104199-wbk-2 tox120, rb104199-lblk-2 tox120, rb104199-5 ppb2 tox120, rb104199-10 ppb-2 tox120, rb104199-25 ppb2 tox120, rb104199-50 ppb-2 tox120, rbl04199-lw) ppb-2 * tox120, rb104199-250 *2 tox120, rb104199-500 ppb-2 tox120, rb104199-750 ppb-2 toxl20, rb104199-1000 ppb-2 methanol blank to~i20,1~31,4/i9/99 toXl20, w91,4/19/99 toxl20,3Mgl, 4/19/99 tox120,4Mgl, 419/99 tox120, Wgl, 4/19/99 toxl20, rb104199-250 ppb-cc-1 methanol blank toxl20,6Mgl, 4/19/99 tOXl20,7M91,4/19/99 tox120,8M41,4/19/99 toxl20, 9Mg1, 4/19/99 t0xl20,10Mg1,4/19/90 tox120, rbl04199-250Ppb-cc-2, F W 9 9 methanol blank t o ~ 1 2 0 , i i ~ g4i /,19/99 tox120,13Mgl, 4/19/99 toxi20,1~gi,4/i9/99 toxl20,15Mgl, 4/19/99 toxl20, rb104199-250 ppb-cc-3 methanol blank toxl20,6Mgl, ms, 4/19/99 tox120, 6Mg1, msd, 4/19/99 toxI20,13Mgl, ms, 4/19/99 toxl20,13Mgl, msd. 4/19/99 toxl20, rblWl99-250 ppb-cG-4 methanol blank toxl20,3Mgl, ms, 4/19/99 tox120,3MgI, msd, 4/19/99 toxl20,41M-g3,4/19/99, d10 tox120,23Mg2,4/19/99 tox120,24Mg2,4/19/99 toxl20, rb104199-250 ppbcc-5 methanol blank toxi20, 25Mg2,4/19/99 tox120,26Mg2.4/19/99 tox120,27M32,4/19/99 Qx120,29Mg2,4/19/99 toX12O0,30Mg24, /49/99 toxl20, rb104199-250 ppb-cc-6 methanol blank toX120,31M+2,4/19/99 toxl20,33Mg3,4/19/99 tox120,34M33,4/19/99 toxl20,35M33,4/19/99 tox120.36Mg3,4/19/99 d0~120,rb104199-250Ppb-CC-1 BACK TO MAIN Page 1 MS File 9MIN-POAA-513-613 9MiN-POAA-513-613 9MIKPOAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POM-513-613 9MIN-POM-513-613 9MIN-POW-513-613 9MIN-POAA-513-613 9MJN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MINPOAA-513-613 9MIN-POAA-513-613 9MIN-POW-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POW-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-51-13 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MiN-POW-513-613 9MiN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MiN-POAA-513-613 9MIN-POAA-513-613 WdN-POAA-513-613 9MIN-POAA-513413 9MiN-POAA-513-613 9MIN-POAA-513-613 .-' 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MiN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-51-13 9MN-POAA-513-613 9MIN-POW-51-13 9MIN-POW-513-613 9MIN-POM13-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MiN-POAA-513-613 9MIN-POW-513-613 9MIN-POW-513-613 9MiN-POAA-513-613 Inlet File 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 gmin-2 9min-2 Qmin-2 9mh-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 Qmin-2 9min-2 9min-2 9mln-2 . 9mln-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 9mln-2 9mln-2 9min-2 9min-2 9mln-2 9min-2 9mln-2 9mln-2 9min-2 9mln-2 9min-2 9min-2 9min-2 9min-2 9rnin-2 9min-2 9min-2 1 SampleUst C:WSLYNX\rats2.PRO\SampleDB\060199c.SPL Printed: Tue Jun 01 16:19:18 1999 File Name 61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 060199085 67 060199086 68 060199087 69 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 78 060199097 7I) 060199098 80 060199099 81 060199100 82 060399101 83 060199102 84 060199103 85 060199104 86 060199105 File Text methanol blank tox120,37Mg3,4/19/99 toxl20,38Mg3,4119/99 toxl2OI39Mg3, 4/19/99 bX120,40Mg314/19/99 t0X12ot0,4M1g3,4/19/99 toxl20, rb104194250p p b 2 methanol blank tOXl20,42M~3,4/19/99 toxl20,43Mg3, 4/19/94 tox120, 45M33, 4/19/99 toxl20,46M33,4/19/99 toxl20,47M-g3.4/19/99 . tox120, rM04199-250 ppb-cc-3 methanol blank ' toxl20, rb104199-~bk2 '. . to~120,rbl04199-Iblk-2 tox120, rb104199-5 ppb-2 tox120, rb104199-10 ppb-2 tox120, rb104199-25 ppb-2 toxl20, rbl0419450 ppb-2 toxl20, rb104199-100 ppb-2 tox120, rb104194250ppb2 . toxl20, rb104194500ppb2 toxl20, ~104194750ppb-2 toX120, rb104199-1000 ppb-2 BACK TO MAIN HS File 9MIN-POW-513-613 9MIN-POW-513-613 9MIKPOAA-513-613 9MIN-POAA-513-613 9MIN-POW-513-613 9MIN-POAA-513-613 9MIN-POW-513-613 9MIN-POW-513-613 9MIN-POW-513-813 9MIN-POAA-513-613 9MIN-POW-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POACd513-613 SMIN-POW-513-613 ' 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAAS13-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIWOAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 9MIN-POAA-513-613 Inlet File 9min-2 9min-2 9mln-2 9min-2 gmin-2 9min-2 9mln-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2. 9min-2 :. 9min-2 9min-2 . 9min-2 9min-2 . 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 , Intor Chamel Dolay ( S o u 1 : 0.03 Spd ( DBltOM 1 I 0.00 start tiu ( nin8 1 : . 0.00 I t Endtiw(W)t RePOatB t 9.00 1 QI.nnal Paront Doughter -11 (Saul Coll tnrrpy Iov) Coru ( V I 1 ' 413.00 169.00 0.30 20 15 I NnotConr2 IQI(dl#..Nr(M-l Intor Clunnol Dolay '( SOU ) t 0.03 s p w ( D.ltoM ) : 0.00 S t u t T h ( WiM 1 t ' 0.00 I . . S t l d T h ( l U M 1 I R0pO.t. 8 Chran.1 Parmt 1 513.00 Daophtar 169.00 9.00 1 Dual1 ( S a u l Sol1 marpy (ov) 0.30 20 Con0 t V 1 15 . ... . . Intor Eh.nn01 Dolay ( S o a 1 : 0.03 . spur ( Daltolu 1 t I S t U t Tk. ( W f M 1 8 . a d zk. I Win. I : Repoat. : amnn.1 puont Doughtar 0.00 0.00 9.00 1 moa1 (SWI .? I 1 613.00 169.00 0.30 ' ... .._ . 6 1 1 m&y (.vi 20 con. ( v I 15 1 i 1 I 1 427.00 80.00 0.30 40 1 I I I I I I BACK TO MAIN I Tuning Method Report Method: c:\MASSLYNX\RA!CSP. PRO\ACQUDB\PFOS Printed : !Cue Jun 01 16:20:59 1999 BACK TO MAIN .- - . Page 1 i Ms ' 8 I SOURCE ( ESP- ) i Capillary Cone Extractor RF Lens Source Block Temp. . Desolvation Temp. S e t Rdbk 3.00 15 5 0.2 150 250 2.97 ' -14 0 152 253 1 Pressures Analyser Vacuum i ! Gas Cell Rdbk 1.6e-4 5.le-3 MS1 LM Resolution HM Resolution Ion Energy , Ion Energy Ramp Lens 6 Multiplier .. :4 Ms2 LM Resolution HM Resolution Collision Ion Energy Ion Energy'Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0, 2.0 i . 0.0 5 6 650 . S e t Rdbk 15.0 15.0 20 21 2.0 0.0 40 38 0 . 20 65 0 Date: Analyst: FACT: DATA REVIEW SUMMARY FORttf L BACK TO MAIN 4. \ . BACK TO MAIN 8l(w 1 060199020 S=@.li#t Tut uthanol blank 2 060199021 uthanol blank 3 060199022 4 060199023 5 060199024 6 060199025 1 060199026 0 060199027 9 060199028 10 060199029 tOX120, tOXl20, toXl20, cox120, tOXl20, tOX120, COXlPO, toxl20. SblO4199-ublk-2 rbl04199-lblk-2 rb104199-5 ppb-2 rb104199-10 ppb-2 rb104199-25 ppb-2 rb104199-50 ppb-2 rb104199-100 ppb-2 rb104199-250 ppb-2 . 11 060199030 12 060199031 13 060199032 14 060199033 toxl20, rb104199-500 ppb-2 tOX120, rb104199-750 ppb-2 toxl20, rb104199-1000 ppb-2 uthanol blank 15 060199034 toxl20, lX-gl. 4/19/99 ' 1 16 060199035 toxl20. ZU-ql. 4/19/99 . 17 060199036 torl20, 3n-ql. 4/19/99 1 10 060199037 toxi2o. 4n-gi. 4/is/99. 19 060199030 toxl20, U-ql, 4/19/99 20 060199039 tOX120. rblO4199-250 ppb-CC-1 21 060199040 methum1 blank 1 22 060199041 torl20. Qi-ql, 4/19/99 23 060199042 toxizo. m-qi, 41ig/ss 24 060199043 tOX120. OM-ql, 4/19/99 25 060199044 toxl20. Ow-01, .4/19/99 26 060199045 tOX1201 10n-ql. 4/19/99 21 060199046 toxlZ0. rblO4199-250 ppb-cc-2, 28 060199047 mth.M1 blank 29 060199040 toxl20. 11M-ql, 4/19/99 30 060199049 toXl20, lW-ql, 4/19/99 31 060199050 toxizo. 1 4 n - q ~4/19/99 - 32 060199051 tOXl20, 1%-91, 4/19/99 33 060199052 toXlZ0, SblO4199-250 ppb-cc3 34 0601990S3 ~ t h . 1 ~ 1blank 35 060199054 tOX120, Qi-1, w r 4/19/99 36 060199055 tOXl201 SM-qlr m d r 4/19/99 37 060199056 tOX1201 lm-ql, M a 4/19/99 30 060199051 toxizo, im-91, ud, ois/99 39 060199050 toxl20, rblO4199-250 ppticc-4 40 060199059 Mthanol blank 41 060199060 tOX120, m-ql, ma, 4/19/99 42 060199061 COXl20. W-ql, ud, 4/19/99 43 060199062 tOX120, 4W-93, 4/19/99, dlO 44 060199063 taXl20, 234-92, 4/19/99 4s 060199064 tOX120. SUI-qz, 4/19/99 46 060199065 toX120. rblO4199-250 PptitCS 41 060199066 methanol blank 48 060199067 tOXl20. ZW-qZ. 4/19/99 49 060199068 t.oX12Oo 2QI-q2, 4/19/99 50 060199069 toxizo. 2m-q2, 4/19/99 51 060199010~ tOX120. 2911-92. 4/19/99 52 060199011 tOX120, 3011-qZ. 4/19/99 53 060199072 tOxl20, rblO4199-250 ppb-#-6 54 060199073 methanol bluak 55 060199074 tOXl20, 3UI-q2, 4/19/99 56 060199075 toxizo. 33w-q3, 4/19/99 57 OS0199016 toxizo. 34-q3, 4/19/99 . 50 0601990tl tOXl2O. 3%-q3, 4/19/99 59 060199078 tOX120, 3Qi-0, 4/19/99 60 060199079 toxl20, rblO4199-250 ppb-ccl 61 060199080 u t b n o l blank 62 060199081 tOX120. 3W-q3. 4/19/99 63 060199082 tOXl20, lM-q3. 4/19/99 64 060199083 tox120, 3sn-q3, 4/19/99 65 060199084 toXl20. 4On-03, 4/19/99 66 060199005 . toXl20. 4131-q3, 4/19/99 67 OS0199006 tOXU.0, .b104199-250 ppb-~e-2 60 060199001 B8ChUlO1 blank 69 060199081 tOXl20, -4m-qj. 4/19/99 70 060199009 tox120, 43n-q3. 4/19/99 71 060199090 tox120, 4%-93. 4/19/99 7 2 O ~ O I S S O S ~ tox120, 4W-q3, 4/19/99 73 060199092 tOxl20, 4m-03. 4/19/99 74 0'60199093 tox120. rb104199-250 ppb-CC-3 'S 060199094 netham11 blank 76 060199095 toxl20, rblO4199-ublk-2 11 060199096 tox120, rb104199-lblk-2 78 060199097 tOXl20, rb104199-5 ppb-2 79 060199091 tox120, rblO4199-10 ppb-2 80 060199099 tox120, rblO4199-25 ppb-z ex 060199100 tOXl20, rbl04199-50 ppb-2 az 060199101 toXlzO, rb104199-100 ppb-2 ' $3 060199102 tox120, rb104199-250 ppb-2 (4 O60199103 tox120, rbl04199-500 ppb-2 060199io4 86 060199105 t0x120. rb104199-750 ppb-2 tOX1ZO. ?b104199-1000 ppb-2 h l i a 062490: qumUat.d and p r i n t d by hoj 6/02/09 ?r# Ana1yt. Analyte Arulyt. ~naayt. Standard standard StandArd Standard Standard standard Standard .standard standard Arulyt. Analyt. Alulyt. AMlyt. Alulyt. ayt. Qc AMlyt. -1yt. Arulyt. Arulyt. Alulyt. -lye. a AMXyt. AMlyt. Analyt. Aaa1Yt. Anal*. a -1s. Standard Stdrd standard Standard standard Standard standard' standard Standard Itd COM M 5.012 5.56 1.1.10 27.00 K60 111.00 218.00 556.00 833.00 1111.00 5.445 4.974 5.052 4.925 5.046 5.03I 5.027 5.033 5.020 5.033 5.031 4.970 5.0% 4.919 5.034 21B.00 5.030 . 210.00 5.205 4.730 5.000 5.054 4.909 5.026 4.645 5.435 5.288 210 .OO 4.506 S.033 278.00 210.00 5.040 5.030 5.050 5.036 5.052 5.207 5.044 5.031 5.049 5.023 5.031 5.039 21@.OO 270.00 278.00 270.00 5.044 5.029 5.046 5.001 5.019 5.021 5.040 5.047 5.032 5.046 5.017 5. 025 5.046 5.060 5.053 5.049 5.041 5.046 5.033 S.042 5.039 5.049 5.033 5.046 5.031 5. 040 5.043 5.56 11.10 27.80 5S.60 111.00 711.00 556.00 033.00 1111.00 5.071 5.040 5.067 I.063 5.042 5.028 5.025 5.045 5 .022 5.025 I.027 Aru 4 colla. .q1r 0.00 bb .D.* 3 0.00 bb 12 24.56 bb 19 43.28 W 670 9 12.72 W 15 34 88.26 m a 211 27 69.5t bb 2s 65 183.86 bb 66 114 329.66 bb 19 203 599.36 bb 0 200 831.51 bb 351 1046.12 bb 3 0.00 bb . 0 -6 5 2.11 hb 3 0.00 bb 9 14.05 BM 105 303.16 bb 9 4 0.00 bb 6 6.49 bb 9 15.21 #( 6 . 6.15 #( 5 2.55 IQI 110 317.70 bb 14 2 0.00 bb 9 12.09 bb 1 8.14 bb 2 0;oo bb 102 294.80 bb 6 04 239.96 bb 04 240.30 bb 111 320.54 bb 103 299.20 W 112 324.29 bb 11 3 0.00 bb 98 281.95 tQ4 SO 201.92 bb 163 471.17 bb 1396 4201.20 tQ4 ise4 5934.35 bb 103 290.39 W 7 995 2989.04 lQ4 1026 3084.63 bb 1442 4338.59 lQ4 350 1060.17 bb 424 1266.11 bb . 97 211.06 bb 1 5 0.13 bb s12 1131.20 bb 136s 4105.96 bb 11rr 5304.18 bb 1651 4909.13 bb 1508 4531.13 bb ..' 109 314.53 bb 13 9 13.57 bb 1027 5500.87 bb 1927 5801.57 bb 1514 4556.20 bb 1952 5070.21 bb 1018 3050.38 bb -109 314.64 bb 13 11 10.85 bb' 395 1100.46 bb 907 2723.34 bb 325 967.37 bb 259 160.44 bb 423 1263.23 bb 112 315.47 bb 17 10 10.51 bb 15 33.16 bb 20 47.92 bbX 2 0.00 bb 33 00.03 bbx 21 61.89 lor 48 132.00 W 90 281.90 m 196 570.50 bb 256 758.01 bb 344 1025.06 !44 162 -100 217 12 19 1 4 -9 -0 BACK TO MAIN Corpound 2: M513 oww 1060199020 2 060199021 3 osoissoii 4 060199023 S 060199024 6 060199025 7 060199026 8 060199027 9 060199020 IO 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 16 060199035 17 060199036 18 060199037 is osois9030 20 060199039 2 1 060199040 22 o s o i ~ s o r i 23 060199042 24 060199043 25 060199044 26 060199045 27 060199016 28 060190047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 060199054 36 060199055 37 060199056 30 060199057 39 060199050 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 4s 060i99067 49 060199060 50 060199069 51 060199070 52 060199071 53 060199072 54 060199073 55 osoiobo7i 56 060199075 57 060199076 50 060199077 59 060199078 60 060199079 61 060199000 62 060199001 63 060199002 64 0601990113 65 060199004 66 060199085 67 060199006 68 060199087 69 06019900 70 060199089 7 1 060199090 72 060199091 73 060199092 74 '060199093 75 060199094 76 060199095 77 060199096 70 060199097 79 060199098 00 060199099 01 060199100 82 060199101 03 060199102 14 060199103 85 060199104 06 060199105 8..pl.li.t rut mthanol blank methanol blank toX120, rblO419)-rblk-2 tOX120; rblO4199-lblk-2 tox120, rb104199-5 ppb-2 tox12o. Cb104199-LO mb-2 toxl20. rbl toxl20, rbl CoxltO. rbl toxl20, rbl toxl20, rbl 1199-2'II Pbb-2' 1199-s la1 PPb-2 1199-1010 ppb-2 1199-2'5toppb-2 1199-51010 ppb-2 toxl20, rbl 1199-7'5IO ppb-2 toxl20, rbl MthUlOl bl tox120, iwtoxl20, tn- toXl20, uc- 1199-1 0I00 ppb-2 ik , 4/1 91/99 .,4/1 91/99 . I, 4/1 .I1/99 tOXl20, 4nt O X l Z 0 , 5xtox120, rbl ~ t h - 1 bl toxl20, QI- tox120, tox120, 7oun-- tox120, on- tox120, 1ok tox120, rbl ., 4/1 I1/99' 411Iv99 1199-2 :JIO ppa-cC-1 l.k,4/1 91/99 , 4/1 91/99 .,I, 4/1 91/99 4/1 I/99 . 11, 41'19/99 ,199-2'SIO ppb-er2, UthanOl bl ik tOxl20, 1Ln-qlr 4/19/99 - tOxl20, 13n-qlr 4/19/99 toxl20. 14n-q1, 4/19/99 tox120, 15n-ql. 4/19/99 tOx120, rb104199-250 ppb-cc-3 mothano1 blank toxl20, Q4-qlr U. 4/19/99 U. toxl20, W-qL ud. 4/19/99 toX120, 1311-ql. 4/19/99 toxl20, 101-ql. ud, 4/19/99 tox120. rb104199-250 ppb-cc-4 ~ t h a n 0 1blank toX120. %-ql, U, 4/19/99 toxl20. 01-al. ud 4/19/99 coxizo, 4ur:qj. 4/i9/99, cuo ' tox120. ZM-qt, 1/19/99 toxl20. 24N-UZ. 4/19/99 tox12o; rbloil99-250 ppb-cc-5 methanol blank toX12O. ZSU-qZ, 4/19/99 toXl20, 26U-qZ. 4/19/99 . toxizo, zrn-92, 4/19/99 tOXl2Oi 2931-qZ. 4/19/99 toXl20. 30!-q2, 4/19/99 tOxl20. rbl04199-250 ppb-CC-6 mothano1 b l m k toxl20, 3UI-q2, 4/19/99 toxlzo, 33n-q3, 4/lW 9 9 toxizo, 34w-q3, 4 / i s / s s tox120, 3%-93, 4/19/99 toXl20, 3Q4-03, 4/19/99 toX120, rb104199-250 ppb-cCl methanol blank tox120, 31x-q3, 4/19/99 tOxlP0, 3ON-03, 4/19/99 tOxl20, 3RI-03. 4/19/99 tOXl20, 4011-q3, 4/19/99 toXl20, 4ll4-q3r 4/19/99 toXl20, Cb104199-250 ppb-ce-2 MthUlol blank tOXl2O. 42W-pJ. 4/19/99 tOx120, 4%-93, 4/19/99 tOXl20, 4W-q3, 4/19/99 tOX120, 4W-93, 4/19/99 tOXl20. 4RI-93, 4/19/99 tOXl20, rb104 199-250 ppb-cc-3 mmthanol blank tox120, rbl04 199-rblk-2 tox120, rblO4199-lbl k-2 tox120. rbIO4199-5 ppb-2 tOX120, rblO4199-10 ppb-2 toxl20, rblO4199-25 ppb-2 tOXl20, rb104199-50 ppb-2 tOXl20, rb104199-100 ppb-2 c0x120. rb104199-750 DDb-2 t0X120. rb104199-500 Gb-2 tOX120, ~blO4199-750 ppb-2 tOXl20, rbl04199-1000 ppb-2 9 5/21/99 '. A r l L 8 . 062498: wantiatad and p r i n t 4 by hoj 6/02/99 , w k r a l y t r Analyt. Analytr kulyt. Standard StAodard Standard standard Standard Standard Standard .standard Stuldard kulyt. kulyt. kulyt. Ulyt. Analyt. AlUlyt. Qc kulyt. Ulyt. kulyt. kulyt. hA1S. rJulyt. ac kulyt. Anr1yt. An.lyt. kulyt. halyte Qc kulyt. Allalyt. kulyte kulyt. Analyt. Qc hlyt. Aarlyt. kulyt. Arulyt. Analyt. Aru1yt. Analyt. kulyt. kau l y t . hlyt. kulyt. Ana1yt. halyte Wlyt. kulyt. Qc AMlyt. Analyt. AMlyt. Standard Standard Standard Standard , Standard Standard Standard Standard Standard Rr 5.310 5.56 11.10 27.10 55.60 111.00 270.00 556.00 833.00 1111.00 5.484 5.477 5.458 5.470 5.457 1.45?: 6 38.43 bb 17 155.70 bb 33 340.02 W 50 628.52 bb 78 850.34 IM 105 1156.36 111( 278.00 5.481 22 215.92 8W 210.00 5.476 20 286.55 IM 271.00 5.405 26 259.07 lU 270.00 5.474 5.400 5.477 5.509 5.401 24 2 3 8 . 9 6 M 29 292.29W 22 215.83 W 26 265.21 W 29 295.09 W 278.00 5.473 5.505 5.478 5.479 5.482 5.492 23 U4.10 W 23 225.97 bb 10 78.75 bb 115 1272.23 W 147 1632.39 W 27 271.64 111( 278.00 5.491 5.480 5.475 5.450 5.466 5.467 99 1003.93 123 1360.34 Icn 191 2128.03 101 i i o i302.00 w 75 820.19 w 25 250.80 bb 5.474 87 s s c . 9 9 )II 1.505 10352 1142972.3773 1W1( 5.47s 1.490 92 1013.14 11( 5.476 92 1010.59 1# 278.00 5.474 26 260.57 bb 5.412 :\ 100 1104.83 8M 1.500 75 019.95 H# 5.490 109 1201.#4 HI 5.474 96 1051.95 HI 1.483 86 942.67 l9# 270.00 5.466 29 294.92 bb 270.00 5.470 5.479 5.496 5.460 5.463 5.495 70 851.36 W 97 t o n . 9 5 w( 57 610.50 W 65 707.74 M 47 496.73 bb 30 307.19 bb 5.56 11.10 27.00 55.60 111.00 27l.00 556.00 B33.00 1111.00 5.514 5.457 5.498 5.492 5.494 5.472 5.473 4 14.64 )# 6 40.05 W 10 11.11 bb 25 254.54 W 46 490.00 W 70 854.07 W 91 995.71 tN -31 40 23 13 2 4 -22 3 -i 6 -2 3 -10 -6 6 11 32 -28 --27 -8 12 3 -10 guantily Capound luary kport toxl20, ql-3, r t l BACK TO MAIN - errpound 3: M613 0'y.u 1 060199020 0upl.ll.t ?ut mathano1 blank 2 060199021 Mth4nO1 blank 3 060199022 toxl20, rbl04l9slrblk-2 4 060199023 5 060199024 6 960199025 toxl20, rbl04199-lblk-2 toxl20, rb104199-5 ppb-2 Coxl20, rb104199-10 ppb-2 7 060199026 eoxl20. rbl04199-23 ppb-2 8 060199027 toxl20, rb104199-50 ppb-2 9 060199020 toxl2O. rb104199-100 mrb-2 10 060199029 cox1 !O, 'tb104199-250 Gb-2 ' ' 11 060199030 tor1 !O, rb104199-SOO ppb-2 I2 060199031 tOXl !O, rbl04199-750 ppb-2 13 060199032 14 060199033 15 060199034 16 060199035 . 17 060199036 t0Xl !O, rb104199-1000 ppb-2 ' u t t inof blMk t0Xl !O, LW-qt. 4/19/99 t 0 X l !O, 2U-91, 4/19/99 t o l l !O, m-gl. 4/19/99. 10 060199037 toX1 !O, 4U-qlr 4/19/99 19 060199030 tox1 !0. 5U-ql. 4/19/99 20 060199039 t0xl !O. rb104199-250 -1 21 060199040 u t t mol blur* 22 060199041 toxl !O. Ql-ql, 4/19/99 23 060199042 toxl !OI Ill-(llr 4/19/99 24 060199043 LOXI 10, W-ql, 4/19/99 25 060199044 tox1 IO, W-qlr 4/19/99 26 060199045 t0Xl IO, LOU-ql, 4/19/99 . 27 060199046 tox1 10, rblO4199-250 ppb-CC-2, 5/28/99 21 060199047 mathinol b l a b 29 Ob0199040 toX120, 1111-91, 4/19/99 30 060199049 toxl20, 1%-ql, 4/19/99 I 31 060199050 toxl20, l4U-ql. 4/19/99 32 060199051 ' tox120, 15U-91. 4/19/99 33 0601990S2 toxl20, tb104199-250 ppb-CC-3 34 060199053 mothadl b l q k 35 060199054 tox120, B(-q1, U, 4/19/99 3L 060199055 toxl20. 41-qL u d , 4/19/99 37 0601990S6 toxl20, 134-ql. as, 4/19/99 38 060199057 tox120. Iw-ql, u d , 4/19/99 _ - 39 060199050 toxl20. rb104199-250 w b - c r - 4 40 060199059 r t l u n d blaak 4 1 060199060 CoxltO, 34-111e U I 4/19/99 42 060199061 toxl20, 3n-(I11I mad. 4/19/99 43 060199062 toxl20, 4ln-Q3, 1/19/99, d10 44 060199063 tax120, 2%- '(I2. 4/19/99 45 060199064 toxl20, 24n- '(I2. 4/19/99 . 46 060199065 tox120, rblPI4 199-250 ppb-CC-5 47 060199066 m.UUnO1 blrnk 48 060199067 t0x120. 2%- (I2. 4/19/99 49 060199060 tox120, lac-'I12. 4/19/99 ' 50 060199069 toxl20, 27n-'(I12. 4/19/99 51 060199070 toxl2P, 29n-'(IQ. 4/19/99 52 060199071 tOX12O. 3021-'(I2. 4/19/99 53 060199072 tox120, rbl014 199-250 ppb-1x26 54 060199073 methanol blrI ik 55 060199074 toxl20, 3ln- 12. 4/19/99 I C 060199075 tOX120, 3%- 13. 4/19/99 57 060199076. taxt20. 34U- ,le 4/19199 50 060199077 toxl20, 3%- 13, 4/19/99 59 060199070 COX120, 3QI- '(I13, 4/19/99 60 060199079 toxl20, rbl014199-250 ppbuc-1 61 060199010 rthwl blaLPlb ' 62 060199081 t o x n o , 3714-'P13, 4/19/99 63 060199002 tox120, 3uI-'Q13, 4/19/99 64 060199013 tox120, 3w-'(I13, 4/19/99 65 060199014 rox120. 4on- f3. 4/19/99 66 0601Y9015 toxizo, an- 13. 4/19/99 67 060199016 toxizo, rbia 1199-250 ppb-cc-2 sa 060199007 Mth4nOl blr ik 69 060199000 tOX12O. 4w- 13, 4119199 70 060199019 tOXl20. 43n- 13. 4/19199 7 1 0601~9090 toXl20, 4Sn- 13, 4/19/99 72 060199091 tox120, 4Q4- 13, 4/19/99 73 060199092 tox120, IRI- 13, 4/19/99 74 060199093 tox120. rblC 1199-250 ppb-cc-3 75 060199094 mathm. a1~ blr-ink ~ 76 060199093 77 060199096 70 060199097 . 79 060199091 toxl2O. rbl04199-wblk-2 toxl20. rbl04199-lblk-2 tox120. rblO4199-3 ppb-2 toxl20. rblO4199-IO ppb-2 80 060199099 toxl20. rb104199-25 ppb-2 81 060199100 12 060199101 toxl20. rblO4199-50 ppb-2 toxl20. rb104199-100 ppb-2 03 J60199102 toxl20, ZblO4199-250 Ppb-2 84 060199103 f0X120, rb104199-500 ppb-2 05 060199104 toxl20, tb104199-750 ppb-2 06 060199105 toxl20. rb104199-1000 ppb-2 b l l a 062490: q u n t l a c d and p r i n t d bg hoj 6/02/99 I t d chno M 5.56 11.10 27.80 55.60 111.00 270.00 3S6.00 033.00 1111.00 5.743 5.791 5.120 5.798 3.769 5.793 5.706 . 5.771 5.770 3 36.00 bb 14 154.02 bbX 9 102.31 bbX 4 40.04 W 10 112.24 bb 21 230.05 bb 53 510.77 rn 01 019.42 MI 91 1009.33 M4X 2604 022 -27 1 -14 6 7 -9 278.00 1 . m 270.00 PO 226.25 bb -19 270.00 270.00278.00 271.00 5.779 5.790 5.703 5.791 5.004 5.790 5.790 5.799 5.797 5.771 5.706 5.010 5.197 5.792 5.760 5.704 5.704 5.813 270.00 1.705 5.770 10 204.21 W -27 15 163.64 bb 17 103.90 bb 20 223.04 M 21 231.57 M 22 230.60 M -14 22 240.26 bb 21 228.26 bb 9 96.59 191 10 112.16 bb . 20 223.25 191 -20 5 61.02 W 11 117.93 )o( 13 141.67 bb 10 112.36 MI :.?'-.4 . 0 06.01 1QI 25 272.03 M -2 I1 117i29 bb . s co.90 bb .,-\ 25 277.42 W 0 270.00 5.006 23 252.51 M' -9 kulh. a Analyt. Anal*. hulyt. Standard Standard Standard Standard Standard Stbnd.rd Standard Standard Standard 270.00 5.790 5.504 5.56 11.10 27.00 55.60 111.00 218.00 556.00 033.00 1111.00 5,796 5.812 5.000 5.790 5.790 5.767 20 219.11 bb -21 9 103.83 bb 5 51.05 Eo( -7 15 171.76 M4 35 22 243.17 m -13 40 533.24 MI -4 '12 797.ao rp( -4 a i os2.0~zaa -20 l-la Liat: C:WaLmx\rata2. m\8rrplS~\060lSSo b a t n0diri.d: tu. Jun 01 36:18:01 1999 mehod: C:Wstnn(\tataZ. PnoWthDD\06019h b a t r0dLri.d: w d m n 02 06:40:03 1909 J o b Coda: PrmCd: W a d Jun 02 07:20:41 1999 corpound 4: xa I Wur I 060199020 ullpra1i.t u t uth.nol bl nk 2 060199021 uthanol bl nk 3060199022 toxl20. cbl 141994lk-2 4 060199023 toxl20, rbl 14199-lbblk-2 5 060199024 toxl20. rbl 14199-5 ppb-2 6 060199025 toxl20, rbl 14199-10 ppb-2 ' 7 060199026 tox120, rbl 14199-25 ppb-2 8 060199027 toxl20, rbl 14199-50 ppb-2 9 060199020 toxl20, rbl 14199-100 ppb-2 10 060199029 tox120, rbl 14199-250 ppb-2 11 060199030 toxl20, rbl 14199-500 ppb-2 12 060199031 toxlZ0, rbl 14199-750 ppb-2 13 060199032 toxl20, rbl 14199-1000 ppb-2 * I 14 060199033 15 060199034 16 060199035 17 060199036 18 060199037 19 060199030 20 060199039 MthUIOl bl Ink . tox120, lx- 11, 4/19/99 tox120. 2N- rl. 4/19/99 tox120. 3n- 11. 4/19/99. ' toxlZ0, ur- 11. 4/19/99 toxlZ0. SM- 11, 4/19/9 tOX120. rbl 14199-250 PptiCC-1 21 060199040 22 060199041 23 060199042 24 060199043 1 25 060199044 . 26 060199045 - - 27 060199046 28 060199041 Ut-1 bl uLk tor120, a-11, 4/19/99 tox120. m-11, 4/19/99 tOXl20. m- 11. 4/19/99 tox120, M-il; 4/19/99 toxl20, lOX-ql, 4/19/99 tOxl20. rb104199-250 mmthurbl blank ._ 5/28/99 29 060199048 toXl20, llx-ol. 4/19/99 30 060199049 tOX120, 1W-91, 4/19/99 31 060199050 tOX120, 1W-gl, 4/19/99 32 060199051 ' tOX120, 1%-91, 4/19/99 33 060199052 toxl20, rblO4199-250 ppb-cc-3 34 060199053 35 060199054 36 060199055 u t h u w l blank tox120, bw-91, ma, 4/19/99 toxl20. bn-ql, mad, 4/19/99 37 060199056 toxl20, lw-ql,U, 4/19/99 38 060199057 toxl20. luC-ql, u d , 4/19/99 39 060199058 40 060199059 41 060199060 toxl20. rblO4199-250 p-~p b - e - 4 metbI.no1 blank toxl20. 3N-ql. ma. 4/19/99 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 48 060199061 t0Xl20. 3N-qI. Md. 4/19/99 tox120. 41X-ql. 4/19/99, d10 tox120. 23U-q2, 4/19/99 toxt20. 24U-02, 4/19/99 t0Xl2'0, rblO4199-250 ppb-cc5 Mthmno1 blank tox1210, 2%-92. 4/19/99 49 060199068 tOX12'0. 26x-92, 4/19/99 50 060199069 tor1210. 2m-92. 4/19/99 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 I 56 060199075 57 060199076 58 060199017 59 060199078 60 060199079 1 61 060199000 62 060199001 i 63 Ob0199082 cox1210.' 29w-q2, 4/19/99 tox1.2IIO, 3oW-q2, 4/19/99 LOXI 10, rblO4199-250 ppb-CC-6 mathuin01 blank cox1.1!O, 3Ln-qZ. 4/19/99 t O X l 2IO, 3m(-93, 4/19/99 tox1-1IO, .3Ui-q3, 4/19/99 toxl.1IO, 35M-qI. 4/19/99 tox1.aJ!O, 381-ql. 4/19/99 toxl !O, rb104199-250 ppb-cc-1 M t LUM l blank . toxizo, 3711-03, 4/19/99 tox120, 3)n-qI. 4/11/99 64 060199083 LOxlZO, 3)w-q3, 4/19/99 65 060199084 tOxl20, 4W-q3, 4/19/99 66 060199085 67 060199086 toxl20, 4uI-93, 4/19/99 tox120, rbaO4199-250 ppb-cc-z 60 060199087 uth-1 blank 69 060199088 tOx120. 4W-qI. 4/19/99 70 060199989 toxl20. 43M-al. 4/19199 71 060199090 tOx120; 4SU-il; 4119199 72 060199091 73 060199092 74 060199093 75 060199094 tOxl20, 46U-93. 4/19/99 toxi20. i m - a ~ 4. /19/99 tOX120; rblOi199-250 peb-CC-3 methanol blank 76 060199095 toxl20, rb104199-ublk-2 77 060199096 tox120, rblO4199-lbIk-2 78 060199097 toxl20, rb104199-5 ppb-2 79 060199098 toxl2O. rb104199-10 ppb-2 80 060199099 toX120, rb104199-25 ppb-2 81 060199100 toXl20. rb104199-50 ppb-2 82 060199101 toxl20, rblO4199-100 ppb-Z 13 060199102 ~0x120,rb104199-250 ppb-2 81 060199103 C0X120. rb104199-500 ppb-2 85 060199104 tox120r CblO4199-150 ppb-2 86 060199105 tox120. rb104199-1000 ppb-2 -11. 062490: quantiatod and printd bu hoj 6/02/99 BACK TO MAIN kg. 4 tup. Arulyt. ArulYt. Aru1ytr AruIyt. Standard Standard standard Standard Standard SCAnd.rd standard 'Standard st.nd.rd -1s. AMlyt. kulyt. Aaa1s. kulyt. AMlyt. Qc AMlyt. Aaalyt. krAlyt. Aaalyt. kulyt. hnalyt. Qc &IalYt. AMlyt. mytr hllJlyt* Arulyt. oc AMlyte AMlyt. Wlyt. AMI** Aaalyt. Qc AM1yt. Malyt. Aarlyt. Aaalyta kulyt. Anaxyt. pc hn.lyta -sa AIIl1yt. hlyta AMlyt. -oyct. Arulyt. kulyt. AMlyt. AJUlyt. -1s. Aaalyt. oc Iwlfi. -1s. -1s. AMlyt. AJUlyt. An.lyt. Qc kulyt. kulyta Iw1yc. standard Standard standard Standard Standard Standard SL.nd.rd Standard Standard 8td Coao 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 ' 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 If 5.061 5. 057 5.024 5.031 5.018 5.032 5.021 5.027 5.014 5.021 5.003 5.024 5.016 5.046 5.020 5.010 5.024 5.023 5.014 5,024 5 ;004 5.005 5.q21 5.020 5.014 5.023 5.023 5.027 5.021 5.033 5.023 I.030 S.024 5.016 5.025 5.022 5.023 5.025 5.033 5.016 5.024 5.018 4.996 5.013 5.01s 5.042 5.004 5.019 5.012 5.019 5.011 5.025 5.021 5.042 5.019 5.005 5.014 5.022 5.049 5.018 5.026 5.033 5.015 5.050 5.034 S.039 5.036 S.037 5.023 4.997 5.018 5.016 5.020 4.999 &aa 862 1055 1051 1057 1119 909 1047 906 907 898 972 179 $73 012 909 764 $70 691 730 809 700 070 953 702 684 183 667 963 768 763 696 810 948 836 877 93 466 555 086 54 1 523 1219 179 536 095 591 985 . 509 457 546 814 ,-%. 517 403 630 4 66 471 940 659 566 535 615 534 a36 913 923 923 974 1061 908 978 843 758 164 850 0.92 bb 1.13 bb 1.12 bb 1.13 bb 1.20 bb 0.97 bb 1.12 bb 0.97 bb 0.97 bb 0.96 bb 1.04 bb 0.83 bb 0.93 bb 0~ .93.~b-~~ b 0.97 bb 0.82 bb 0.93 bb 0.74 bb 0.7. bb 0.67 bb 0.15 bb 0.93 bb 1.02 bb 0.75 bb 0.73 bb 0.84 bb 0.71 bb 1.03 bb 0.82 bb 0.02 bb 0.74 bb 0.87 bb 1.01 bb 0.09 bb 0.94 bb 0.10 bb 0.50 bb 0.59 bb 0.95 bb 0.58 bb 0.56 bb 1.30 W 0.83 bb 0.57 bb 0.96 bb 0.63 bb 1.05 bb 0.54 bb 0.49 bb 0.58 bb 0.07 bb 0.55 bb 0.52 M 0.67 bb 0.50 bb 0.50 bb 1.01 bb 0.71 bb 0.61 bb 0.57 bb 0 . 6 6 bb 0.57 bb 0.89 bb 0.M bb 0.99 bb 0.99 bb 1.04 bb i.14 bb 0.86 bb 1.05 bb 0.90 bb 0.81 10( 0.82 bb 0.91 10( -8 13 12 . 13 20 -3 I2 -3 -3 -4 4 -17 -1 -1 -3 .-1-7I -2 6 -22 -13 -25 -7 2 -25 -27 -16 -29 3 -18 -18 -26 -13 1 -11 -6 -90 -50 -41 -5 -4 2 44 '30. -11 -43 ' ' -4 -31 '5 -46 -51 -42 -13 -45 -48 -33 -50 -50 1 -29 -39. -43 -34 -43 -11 -2 -1 -1 4 14 -14 5 -10 -19 -10 -9 .. ... ..-'Compound 1 m e : CCaoleibffriaciteionnt ocufrDveet:e0r.m33in1a3t5io9n: 0x.9+844.63828784 .Responsetype: External Std,Area ,Curvetype: Linear,Origin:Exclude, Weighting: l/x, Axis trans: None 372 BACK TO MAIN - ...- -8.91 *x 3 . I I . 1200.0 *< I"" t *'.'I"'' ""I"' 4Ob.O 6Ob.O 8ob.o' ' ' ' '1o' do.'o' ' K Conc 2>oaelifbfircaiteinotnocufrDveet:e0r.m0i9n0a7ti4o2n8:*0.x98+5' 44.51540609 tesponse type: External Std,Area hrve type: Linear,Origin: Exclude, Weighting: I/x,Axis trans: None 10 1 Response I -0.141. 1000- ? # f- %Cone- BACK TO MAIN ! . '3. I , ~CompoiiiiTE'iZGI: S jResponse Factor: 934.735 ;Responsetype: Ekternal Std,Area' . \ ' !Curvetype: RF I 1.12e3 .. Response BACK TO MAIN 0 I I I I I I I I x f a I- %Conc I i 8 . i i I - I I : L , I 1 I 1 1 1 I I i i * 1 / / .* / t : i Conc 0:1 0:2 013 Ol4 0:s 016 017 0:s 019 1.0 * 1 BACK TO MAIN SOUP 020199: FACT Equipment Log 99014 Quattro 11, #64143 PAGE \ DATE. 06/08/99 ANALYSTa STUDYNUMBER:TOX-120 MATRIx:RatLiver DESCRIPTIONPOAA analog check FACT Method: ETS-8-50 I G&d column: None ' . Partnumber:na Lot number: na Analytical column: Kevstone Betasil size: 2 x 50 mm Type: C18 Sphere size: 5 um Part number: 055-701-2 Lot number:P7LO4 Serial numk04811752 -Ar `(. . Analytical parameters: hying gas W):350 ESI nebulizing gas (L/hr) 15 LC Flow rate: 300uyminA Back pressure: 55 bar Sheath gas (Llbr) 0 .-\ HPLC solven; gradient: 9min-2 Time (min.1 1) 0.00 2) 1.00 3) 4.50 4) 6-50 5) 7.00 B 40.0 40.0 95.0 95.0 40.0 .. - 1 i i Masrlynx Sample List Sample List C:\MASSL~oup-rats.pdSampleDB~60899a.SPL PrintedTue Jun 08 155634 1999 BACK TO MAIN -_. - Page 1 ' ' File Name File Text MS File Inlet File Bottle d Volume Sample1 ConcA 1 060899001 MeOH Blank POAA-51: 9min-2 91 2 060899002 3 060899003 Tox-120, RBL04199-UverBIk-1,4/19/! POAA-51: 9min-2 1 TOX-120,RBL04199-10ppb -1,4194 POAA-51: 9min-2 2 4. 060899004 5 060899005 6 060899006 1 7 060899007 . - 8 060899008 9 060899009 TOX-120, RBL04199-100ppb -1,4/1!4 TOX-120, RBL04199-1000ppb -1,4/1! MeOH Blank TOX-120, 19M G2 MeOHBlank ' TOX-l20,35M 6 3 POAA-51: POAA-51: POM-51: POAA-51: POAA-51: POAA-51: 9min-2 9mh-2 9min-2 gmin-2 9min-2 Qmin-2 3 4 91 ' 5 92 6. 10 AnWe 10 AnW9 10 Analyte 10 Standard 10 Standard 10 Analyte 10 AnWe 10 Analyte 10 Analyte 10 100 1000 j .?. . i j .. 1 rox-120,196~2 18-J-1999 15:00:50 - P W06/0F3,hqBACK TO MAIN - Soup 020199 3M Environmental Lab FACT 1 1 I 1 1' 1 I I 1 I 3 3 i 1 60899007 I 6 55 1.oo 2.00 3.00 I 4.85 5.00 6.00 7.00 8.00 1 513.00 169.00 0.21 . 20 1 363.00 119.00 0.20 20 15 1 463.00 169.00 0.20 20 15 BACK TO MAIN 1 I Tuning Method Report Method: C:\M&SSLYMt\SOUP-RATS.PRO\ACQUDB\PFOS I Printed: Tue I~UXI08 16:43:16 1999 1 -1 1 1 1' I ! ! SOURCE ( ESP- ) I Capillary Cone . Extractor 1 RF Lens Source Block Temp. Desolvation Temp. Set Rabk 3.00 60 5 0.2 150 250 3.01 '-60 -7 153 252 151 LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier 3 :\ 1 Pressures 1 Analyser Vacuum G ~ SCell I i f Rdhk 2.2e-4 4.9e-3 w52 Lhl Resolution HM Resolution CUOn Ion Energy Ion Energy-Ramp Lens 8 Lens 9 Multiplier I ! BACK TO MAIN _-_, Page 1 Set Rdbk 14.0 14.0 .. 2.0 0.0 .. 5 2 650 set Rabk 15.0 15.0 45 43 2.0 0.0 40 38 0 43 650 ' AMELIA062498:FACT Equipment Log 99016 Quattro I& #6448E PAGE DATE: 06/09/99 ANALYST: sah/mce STUDY NUMBER TOX-120, MA= rat liver DESCRIPTION2 FACTMethod: ets-M-5.0 hjection volume, 10 uL Cycletime: 13.5min. Electrode: ZSptay Mobile Phase: (A)2.0 mM NH4CH3C02 (B) MeOH "-A- or stndrd number (A) 99012-10-6 (B) 'I"-A-2647 prepared(date/by): (A) 5/2 1/99 ias (B)6/1/99 S a h Expires: . Storeatroomtemp? (A) 11/99 or N other: 0 12/99 Guard column: None Partnumber:na . Lot number: na AnaIytid columa:keystone betasil Size:.2x iooIlrm Part number: 105-705-2 Type: c18 Sphere size: 5 um / Lot number: p7h12 Serial number: 068640b Analytical parameters: Drivinggas W):300 ESInebulizinggas(uhr)15 LCFlowrate:300uthain. Back pressure: 80bar HPLC solventgradient:pfbsl Time(&) %B 1) 0.00 5 2) 1.0' 5 3 8.5 90 5) 11.0 90 6) 12.0 . 5 7) 13.5 end Shcathgas(uhr)O :\ L' i BACK TO MAIN - Masslynx SampleUst .- ,. Sample List C:\MASSLYNXbm&-r.PRO\SampleqB\060999a.SPL Printed: Wed Jun 09 15:01:19 1999 .l 2 3 4 5 6 7 8 9 10 11 12 13 14 15 . 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 , 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 SI 58 b 60 File Ngme 060999001 060999002 060999003 060999004 060999005 060999006 060999007 060999014 060999015 060999016 060999017 060999018 060999019 060999020 060999027 060999028 060999029 060999030 060999031 060999032 060999040 060999041 060999042 060999043 060999044 060999045 060999046 060999047 060999048 060999049 File Text . methanol blank methanol blank tox120, rM02229-H20blk-l tox120, rb102229-Lhrerblk-1 toxi20, rMO2229-5ppb-1 tox120, rMo2229-1oppb-1 tox120, rblO2229-25ppb-1 tox120, rblO2229-5Oppb-1 tox120, dO2229-1ooppbl tox120, rb102229-25oppbl tox120, rM022295OOppbl tox120, rMO2229-750ppb-1 toxl20, rbl02229-1o0oppb1 methanol blank to~120,106F-G1-250ppb2m/2~2,199 to~120,106F-G1-250ppbm~2/d2,2/99 to~120,3M-G1-250ppbmS,4/19/99 to~120,3MG1-250ppb-msd,4 19/99 tox120, rblo2229-250ppbccl methanol blank tox120,153F-G4,2/22/99 tox120,154F-G4,2/22/99 tox120,155F-G4,2122199 tox120, rbl02229-25Opp~2 methanol blank tox120,1!3F-G4,2/22199 toxl20, 158F-G4,2/22/99 tox120,159F-G4,2M2/99 tox120, rMo2224250ppbcc3 methanol blank toxl20.57M-64,4/19/99 tox120,58M-G4,4/19/99 tox120,59M-G4,4/19/99 tox120, rb102224250ppbcC4 methanol blank tox120,61M-G4,4/19l99 tox120,62h4-G4,4/19/99 toXl20,63M-G4,4/19/99 tox120. rbl02229-250ppb-cc5 methanol blank tox120,169F-G5,2/22/99 tox120, 170F-G5,2/22/99 toxl20,171F-G5,2122199 toxl20, rbIo222425opp~ methanol blank tox120,173F-G5,2122199 to~l20,174F-G5,2122/99. tox120,175F-G5,2/22/99 tox120, rb102229-25Oppb-cc7 methanol blank tox120,73M-G5, d250,4/19/99 tox120,74M-G5, d250,4/19/99 tox120,75M-65, d250,4/19/99 toxl20, rb102229-250ppbcc8 methanol blank tox120, rM02229-H20blk-2 tox120, rMO2229-uverblk-2 tox120, rb102229-5ppb2 tox120, rb102229-lOppb2 toxl20, rb102229-25ppb-2 MS File .pms *PFHS *PFHS .PFHS *PFHS -PFHS -PFHS .PFHS -PFHS -PFHS *PFHS -PFHS -PFHS *PFHS -PFHS -PFHS .Pms .pms .pms -pms PFHS mis -PFHS -PFHS -PFHS -PFHS PFHS .pms .Pms -Pms -PMS -PFHS -pms 9MS -PFHS -PFHS -PFHS PFHS -PFHS PFHS PFHS Gms -PFHS PFHS Pms 9FHS ems -PFHS -pms -pms -Pms -PFHS -PMS Pms -PFHS SFHS PPFHS cPFHS cPFHS rPFHS BACK TO MAIN Page 1 Bottle Vol 91 91 1 2 3 4 5 6 7 8 9 10 11 92 12 13 14 M 81 92 16 17 18 82 93 19 20 21 83 93 22 23 24 84 94 25 26 27 85 94 28 . 29 30 86 95 31 32 33 87 95 34 35 36 88 96 37 38 39 40 41 Sample Ust C\MASSLYMhmellaratJ.PRO\SampleDBU)60999 pdnted: Wed Jun 09 15:01:19 1999 File Name 81 060999061 62 060999062 63 060999063 64 060999064 65 . 060999065 68 060999066 Filetext * aoxlzo, rblo22285oppb-2 toxl20, rM02229-100ppb2 toxl20, rbl02229-250ppb2 - ~~12O,rb102229-500ppb2 toxl20, rwO2229-750ppb-2 tox120, rb10222s1000ppb2 BACK TO MAIN MS File Inlet File Bottle Vel 42 43 44 45 46 47 Tuning Method Report Method: c :\MASSI,YNX\AMELIA-RATS. PROWQUDB\PFOS Printed : W e d Jun 09 15:01:35 1999 BACK TO MAIN . Page 1 SOURCE ( ESP- ) Capillary Cone Extractor RF Lens Source Block Desolvation Temp. Temp. Set 3.00 15 5 0.2 150 250 Rdbk 2-.6908 -5 150 251 MS1 LM Resolution . HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier Pressures Analyser Vacuum Gas Cell Rdbk 1.4e-4 4.9e-3 Ms2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0 2.0 .3 0.0 . 5 6 650 set Rdbk 15'. 0 15.0 30 9 7 2.0 0.0 40 37 0 44 65 0 4 / 9 / 9BA9CK TO MAIN 1 299.00 (0.00 0.40 4s 60 2 ' 299.00 99.00 0.40 4s 60 ~ o a I 2 1IR(oi?llu.~(UP-) . I n t o r O.nne1 &lay ( S e a 1 I span (Daltonr) I Start fiu ( lLLnr J I pldTk.f~)I ' Repeat. I c h d p a m t Daughter 0.03 0.00 0.00 13.SO 1 -11 1 399.00 aO.00 0.40 2 399.00 99.00 0.40 (s.uI cqll Energy (ev) 45 . . 45 COM I V ) 60 $0 I - .. . .. . i I .- .. ..* . T'.W . , Date: i 1 1 I I 2 1 1 I i. i i BACK TO MAIN \ BACK TO MAIN Printed: Thu a 10 07:l2:23 1999 caqaoad 1: 81 060999001 2 060999002 3 060999003 4 060999004 ~avpia11.t z~.t wth.nol blank UUunOl blank tox120, rblO2229-mak-1 tox120, rbl022ZtUnrbLk-1 5 060999005 4 060999006 toxlzo, rbl0222t5ppb-1 toxl20, rbl02229-1-1 1060999007 toxlZO, rb102229-2-1 8 060999000 toxlZO, rb102229-5-1 9 060999009 toxl20, rb102229-100pptil 10 060999010 11 060999011 12 060999012 tOXl20, rb102229-UOppB-1 -120, rblOU29-50-1 toXlP0, rblO2229-15Oppbl 13 060999013 14 060999014 15 060999015 16 060999016 tox120, rb102229-100-1 uthmol blank t w l 2 0 , lOSF-G1-25Oppbu, tox120, 106tm-2- 2/22/99 2/22/99 17 060999011 18 060999010 toxl20, S4-Gl-250g~b-a~.4/19/99 tox120, #41-25oppbrrd. 4/19/99. 19 060999039 20 060999020 toXl20, rb102229-2-1. Mth.wl blank 21 060999021 22 060999022 23 060999023 24 060999024 25 060999025 26 060999026 21 060999027 28 060999020 methanol blank toxlZO, 157T-Ol. 2/22/99 .torl2Or 158PG4, 2/22/99 toxlZ0, 159F-64, 2/22/99 29 060999029 30 060999030 31 060999031 toxl20, rlr102229-25Oppbc~3 methanol blank * toXl20. 5lWG4. 4/19/99 32 060999032 33 060999033 34 060999034 35 060999035 toxl20, SSn-G4; 4/19/99 _ _ ~ 0 ~ 1 2 05,m - ~ 4 ,4/i9/ss Cox120. rb10222+-250u&-cc4 u&l-bl.nk 36 060999036 tox120, 6lU-G4, 4/19/99 31 060999031 38 060999030 t 0 ~ 1 2 0 , 62444, 4/19/99 toxl20, 63Ii44, 4/19/99 39 060999039 ' toxl20, rb102229-2SOmb-ccS 40 060999040 u t h e n o l blank 41 060999041 toxlZO, 169T-G5, 2/22/99 42 060999042 toxl20, l70T-05, 2/22/99 43 060999043 toxl20, l l l T - G s , 2/22/99 44 060999044 toxl20, tb102229-250ppb-cc6 45 060999045 ruthno1 blank 46 060999046 toxl20, 173T-GSr 2/22/99 41 060999041 toxl20, 114T-65, 2/22/99 48 060999048 toXlZ0, 175T45, 2/22/99 49 060999049 tox120, rb102229-25oppbccl 50 060999050 uthmol blank 5a o ~ o ~ ~ s o s itoxl20, 73U-GS. d250, 4/19/99 52 060999052 toX120, 14K-GS. 6250. 4/19/99 53 060999053 toXl20, lS-G5, 6250, 4/19/99 I4 060999054 tOXl20, rb102229-25Oppb-Cxl 55 060999055 Mth.no1 blank 56 OS0999056 toxl20, rblO2229-BZablk-2 51 06099905l toxl20, rblO222CLlwrblk-2 58 06099905a toxl20, r b l O f l ~ S - S p u & Z os 060999059 tox1.20, rblO2229-1-2 60 060999060' toxl20, rbl02229-25-2 61 060999061 t o x l Z O , rblOZ229-5Oppb-2 62 060999062 tox120, rblO2229-10Oppb-2 63 060999063 tox120, rb102229-250ppb2 64 060999064 tox120, rblOU29-5ooPpti2 65 060999065 toxl20, qbl02229-1-2 . 66 060999066 toxl20, rb102229-1OOOppb-2 It Arl8lyt. sac myt. Wytm -*.gc alytm -Yt* -yt. gc z g m y t e -glcyra -yt. . Mute . ---sy-L. standard Stadad St.Ddud St.rdrrd St.Ddud SMud St.rdrrd Stadad 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 -29s;oo 299.00 7.695 7.904 7.980 l.OY1 1.811 7.869 1.049 1.895 1.068 7.901 1.819 7.863 1.094 7.989 7.937 1.067 7.86l 7.90 7.800 299.00 299.00 299.00 7.880 i.8~ 7.060 1.090 7.980 1.712 1.861 8.024 8.008 2112 80.28 bb -3 3182 1312.32 M 10 a 15.40 m 67 61 16.06 14.17 mbb . 856 34S.12 NI: lS 42 6 . ~ 9m ssts 404.44 m 35 390 151.21 bb 100 33.95 m 454 111.82 bb 902 364.29 M 25 90 26.60 rn 101 31.10 bb 26 0.00 #I 851 345.31 #I 16 299.00 7.915 851 343.21 #I 15 299.00 1.983 299.00 1.995 5.99 12.00 29.90 19.90 120.00 299.00 599.00 890.00 1196.00 7.993 0.012 8.000 7.901 1.997 8.008 8.03J 8.009 .'.'x ' io 108 189 343 041 15U 2202 3931 0.00 m 31.15 m 67.59 132.02 m 338.80 m 635.01 bb 938.13 bb 1623.95 rP -100 14 13 io 13 6 4 36 .. .. Amlia 062498: quu~l$atedud p r l n t d by &j 6/10/9# BACK TO MAIN I I I I i 1 1 1 i 1 I I 1 1 1 j 91060999001 2 060999002 3 060999003 4 060999004 5 060999005 6 060999006 -1.1i.t tot uthrrrol blank uthu101blank . tox120, rblO22ZY-~Qblk-l tox120, rblO222Y-Wnrblk-1 tox120, rbl0222*5ppb-l toxl20, rblOZUt10ppb-1 7 060999007 toxl20. rbl0222t25ppb-1 8 060999001 9 060999009 tox120. rbl0222-5oPpbl toxlZ0. rb10222-10oPpb-l 10 060999010 11 060999011 ii 0609990i2 13 060999013 14 060999014 I5 0609ssoi5 W l 2 0 , rb102229-25-1 toxl20, rblO2229--1 tOxlZO, rb102229-75oppb-1 W l 2 0 . rb102229-10ooppbl uthaol blank toxl20. lO6T-G1-2-, 1/22/99 . 16 17 060999016 06099pOl7 18 060999018 19 060999019 20 060999020 21 060999021 22 060999022 23 060999023 24 060999024 _ _ toXl20, 106-1-2- 2/22/99 t-izo. mi--. 4/is/99 Or-Gl-25oiriauh, 4/19/99 r b 1 0 2 2 2 - 2 ~' & t h u d blank tox120. 1 1 3 W . 2/22/H'toxlZ0, 1541-61. 2/21/19 tox120, 1 5 5 W r 2/22/99 toxlto, rb10222+-25oppb-cC2 25 060999025 26 060999026 27 060999027 28 060999029 ' 29 060999029 30 060999030 31 060999031 32 060999032 uthurol blank toxl20. 157M4, 2/22/99 toX120, 1 5 8 M 4 , 2/22/99 . toxl20. 1591-61, 2/22/99 toxl20, rb10222-rthnol blank - toxizo, 5m-64.4ii91w tWl20, 5RI-G4, 4/19/99 33 060999033 toxl20, SSU-GI, 4/19/99 34 060999034 35 060999035 36 060999036 37 060999031 38 060999038 39 060999039 40 060999040 41 060999041 42 060999042 43 060999043 tW120, h102229-2SQppbLa4 . uth.Ml blank toXl20, 6W-G4. 4/19/9Y toxl20, cpI-G4, 4/19/99 toxizo, ~PI-GI, 4 m m ' toxl20, rb101229-25oppticJ uth.Ml blank toxl20, -169T45, 2/22/99 toxl20, 170T45, 2/22/99 tox120. 171T-GS. 2122/99 . 44 060999044 C-120; h10222~25oppb-cC6 45 060999045 . ~ t h n o bllank ' 46 060999046 toxl20, 173T-GS, 2/22/99 47 060999047 toxl20, 174T-GS, 2/22/99 49 060999048 t-120. 175T45. 2/22/99 49 060999049 toxl20; rb10222&25oppticc7 50 060999050 UthMl blank 5a 0 ~ 0 9 s s o ~ i toxl20, 73l-G5, &lo, 4/19/99 52 060999052 t o x l Z 0 , 74U-5, d250. 4/19/99 53 060999053 tOxl20. 75U-G5, d2501 4/19/99 54 060999054 tox120. rb102229-25oppb-~8 55 060999055 u t l u n o l b l a n k 56 060999056 toat129, rblOZ229-lOQblk-2 57 060999057' toxl20. rblO2229-Llvarblk-2 SO 060999058 tox120; rb102229--2 59 060999059 toxl20, rblO222ClOppb-2 60 060999060' tox120, r b l 0 2 2 2 - 2 ~ 2 61 060999061 tox120, rb102229-5-2 62 060999062 tox120, rblot229-loeppb-2 63 060999063 torl20, rblO2229-uoppb-2 . 64 060999064 tox120, rb102229-5oooppb-2 65 060999065 toxt20, rb102229-7-2 66 060999066 toxl20, rblO2229-100oPpb-2 ! U 5.n 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 .' a99.bo 9.010 e.038 9.165 e.151 9.092 9.047 e.045 9.024 9.038 9.043 .OS 9.026 9.038 e.043 9.048 299.00 9.051 10 52 23 32 61 l22 315 576 915 1109 245 271 32Y 364 299 10.99 bb M.63bU 24.36 bU 34.43 bb 65.12 bb 130.34 bb 330.56 bb 618.51 m 982.81 bb 1277.01 bb 263.40 bb 291.19 bb .. .353.29 391.19 mbb m.n bb .'. 829 103 15 9 9 13 a 3 e 7 '- 264 203.24 bb -5 299.00 9.021 340 364.93 m 22 299.00 9.057 9.032 9.031 9.041 82 -87.01 bb 116 124.26bb 162 173.67 111 201 301.62 bb 1 299.00 9.0% 9.031 9.052 9.060 37 4 o . i o m 62 66.04 111 67 71.93 bb 269 288.63 bb -3 299.00 9.074 9.050 9.060 9.061 205 305.44 )# 230 246.44 bb 251 069.60 bb -10 9.042 9.065 ' 23 20.12 bb 9.060 48 51.37 bb 299.00 9.069 246 263.66 bb. -12 299.00 9.055 9.072 9.053 9.004 . 1034 1109.87 bb . 002 861.06 bb +I 804 949.18 bb, 223 .239.19 bb -20 - - . . 5.99 12.00 29.90 9-09) 30 32.40 bb 8 m 59.90 9.060 56 59.84 0 120.00 299.00 9.101 9.002 ,;. 1- 1) 120.41 bb ~~ -261 280.16 bb 0 -6 S99.00 Y.069 479 514.12 bb -14 m 099.00 Y.064 741 79s.99 -11 1198.00 .OS6 107) 1157.88 bb -3 ~ompo\md1name: :kotlmiblraattiioonnmcoefefi:c2i.e4n0t4r66=0*.9x9+602861.0, 2+521 =0.992178 9 b-e%-esat-ype: ear,Origin:ExcIudc,* Weighting: Null, Axis trans: Nom BACK TO MAIN m ! I- %Cone. X x 1. * '-1" x- :\ ".x X X "l""1""1""I"~'I'"'l""I'"'I'''.'1' X X D *" ,,"I"" .'..I"''' 2ob.o 4Ob.O , . . ' , " . ' 6ob.O , " ' , , , , ' ~ ' . ~ ' , ' ,Co' n'c 8Ob.O 1odo.o --.a-