Document 3eBbq80BaQ68Nkx9bvd03Qm3J
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MICROBIOS MICROTOX TOXICITY TEST
TEST SUBSTANCE________________________________________________
Identity: Mixture containing perfiuorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-3017. Current information indicates FC-3017 is a mixture of 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:_________________________________________________________
Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions:
Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 2000 mg/L primary stock solution was prepared by dissolving 200 mg of test substance in 100 mL of Millipore Milli-QTM water. The pH of the stock solution was 6.0, and an osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
RESULTS_________________________ '__________________________
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
002787
Element value:
5-minute EC50 = >1000 mg/L 15-minute EC50 = 785 (728 - 846) mg/L 30-minute EC50 = 558 (541 - 574) mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS___________________________________________________
The test substance 15-minute EC50 for Photobacterium phosphoreum was determined to be 558 mg/L with a 95% confidence interval of 541 to 574 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking = 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and it lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________
This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number N3188, 1995.
OTHER___________________________________________________________ Last changed: 6/26/00.
002788
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ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET
Pii - h e
LR: y Date: Analyst: (- Q T
TEST SUBSTANCE:
f - f C i ~7
1. Preparation of Stock Solution
*Mass/Vol. Test Substance:
*Final Dilution Volume:
*Diluent:
(.('
) ') x ((_
* Final Concentration of Stock Solution:
/V, / ,
2. pH Adjustment of Stock Solution
*Initial p H o fStock Solution: *p H Adjusted to: *p H Adjusted with:
a
3. Osmotic Adjustment of Stock Solution
* Osmotic Adjuster:
/(/'.,( /
Comments: hJ,<J j f r., L W ?.* / 1.
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4. Appearance of pH and Osmotically Adjusted Stock Solution (*CLEAR,' or OPAQUE (circle one) * Color: * Comments:
5. Comments
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MIOROTGX DATA REPORT
FILE NAME. NO l >i>i. K6
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: *'.j : ; e d u r e : BARIO
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36.13/ >', 3 . 8 5 / 3Z . ! o /
6.74 / 85.51/ 83.45/ 30.15/ 7 r; -' 7 /
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35.50 55.86 30.66 50 . 7 8 43.33 43.03 31.90 f; j ; [,
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TEPT DATE: TEPT TIME : !l : 10 A iM A p p r o v e d b y ___ .
CON
125.0000 125 . tJ*10 1) 2 30. (10 0 0 2 5 0 . OOUO 5 0 0 . 0 0 on 5 0 . QOl.-o 100O. Too ; onr.
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100 1000 CONCENTRATION 10E4 10E5
E C 5 0 1 3 0 4 . 9501 (95% CONFIDENCE RANGE;1 1 1 6 . 0 9 6 3 TO 1 5 2 5 . 7 5 9 6 ) # Used f o r c a l c u l a t i o n
.. llV// .00,1;,V' - .. i . I
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i A rIST fCAL SEFoRT FOR DATA FILE N.)i a t .K:.-
SET I MA rNG EOiiATD N' Roc O : : ' . 9 " A A x [, ; r
NUMBEiK OF DATA DA: AD - F D :
REH I E"r!AL VARIANCE: C . oi>:
95V CONFIDENCE FACToR: I 109::i FOR E C
C i x ' x L/ N x 1'X. r 1 : TEN' :
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EFFECT ! VE *N.'NCEN TRA !' I
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EC501304.9501 rog/L (95h CONFIDENCE RANGE: 1116.0963 TO 1525.7596)
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MICROTOX DATA BE' 1.*B:'
: N - 1H3 . K 15 X'' ,,.
v -t ; .iv. - v ....... r i ptio ri : FC - 3(i; /
: BASIC oncent:i:v. M il : 1000 n: 'L,
: 15 mi i:ut.es
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TEST DATE: .. ,,
5
TEST TIME:
A pproved b y ________
O s m o t ic A d . ' meat
D i l u t i o n r .'or :
C o n c e n t r a t e :-n r'=rnt
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GA MMA
A : C
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123 s00 0
: 33 000 0
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V f; :'i000
500 5000
.300 0000
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3
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100 1000 CONCENTRATION 10E4 10E5
KC50 784.8150 (95% CONFIDENCE RANGE: 728.4058 TO .,'845.5927) # Used for-calculations
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STATISTICAL REPORT FOR DATA FILE N3188.K15
ESTIMATING EQUATION: LOG C = 0.9025 x LOG b +2.8948
.'NUMBER OF DATA PAIRS, USED: 6
* <*
.,v<- . .
,
RESIDUAL VARIANCE: i 0.00050
;
1 95% CONFIDENCE FACTOR : 1.07744 FOR EC5Q
CORRELATION COEFFICIENT: R - 0.99724
.COEFFICIENT OF DETERMINATION: R~2 = 0.99450
-EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE):
EC50 = 784.8150 mg/L EC20 = 224.6047 mg/L ECIO = 108.0389 mg/L EC01 - 12.4091 mg/L
( 728.4058 TO ( 203.0035 TO ( 91.2765 TO ( 8.4346 TO
845.5927) 248.5043)**+' 127.8797)***
18.2565)***
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002794
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10
INITIAL DATA
- NUMBER
CONCENTRATION
1 2 3
- .4 V, V 5 . - ;
6. <:i.
125.0000 125.0000 250.0000 250.0000 500.0000 500.0000 1000.0000 1000.0000
fMiil m f"
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GAMMA
% EFFECT ;
0.118
10.53
0.092
8.47
0.296
22.85
0.277
,, 0 .,592 -q : s 9 q
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9.50 22.27 37.16 56.88
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M ICROTOX DATA REPORT
FILE NAME:..N3188.K30
Investigator :
Sample Description: FC-3017
Procedure: BASIC
Initial Concentration : 1000 mg/L
Test Time: 30 minutes
NUMBER
;I0/IT
TEST DATE TEST TIME (Q'-JG//i2Approved by
Osmotic Adjustment: NaCl
Dilution Factor : 2
Concentration Units: mg/L
CONC..
. GAMMA
1 ,, 86 .13A 41 59 '
85.85/42.15
3 ;87.73/ 35.36
4 - 86.74/ 34.98
5 , 85.51/ 25.79
6 83.45/ 25.52
7 80.15/15.18
8 -, . 76.77/ 14.21
CONTROL IT/IO - 53.44/ 95.42
RATIOS =
0.5601
CORRECTION FACTOR = 0.5632
125.0000 ,4: 125.0000 250.0000' 250.0000 500.0000 500.0000 1000.0000 1000.0000 52.52/ 92.72** 0.5664**.
16644 0.14720 0.39744 0.39668 ,' 0.86750 0.84180
1.97391# 2.04294 -'1,
b e s t c o p y a v a il a b l e
STATISTICAL REPORT FOR DATA FILE N3183.K30
ESTIMATING EQUATION: LOG C = 0.8541 x LOG b +2.7464
NUMBER OF DATA PAIRS USED: 6
RESIDUAL VARIANCE:' 0.00012
95% CONFIDENCE FACTOR: 1.02998 FOR EC50
CORRELATION COEFFICIENT : R = 0.99932
COEFFICIENT Of "'DETERMINATION : R `2 = 0.99864
EFFECTIVE CONCENTRATIONS f95% CONFIDENCE RANGE): : '
EC50 = 557.7067 tag/L ( 541.4752 TO EC20 = 170.6819 mg/L ( 160.3766 TO
EC 10 = 85.3864 mg/L ( 77.6362 TO EC01 = l1.0138.mg/L ( 9.0374 TO . " *** EXTRAPOLATE!) RESULTS
574.4247 ) 181.6493)***
93.9104)*** 13.4225)+**
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INITIAL DATA
NUMBER
CONCENTRATION
GAMMA
1
2
3 4 ,5
6
E7
8
125.0000 125.0000 , .250.0000 250.0000 500.0000 500.0000
1 0 0 0 .0 0 0 0
looo.ooob
..
. 0.166 0.147 0.397 0.397
0 .8 6 8 8
0.842 1.974 :V2i';0.43
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% EFFECT.
14.27
12.83 28.44 28.40 46.45 45.71 66.37
67.14
13.55
28.42 -7 ; \i>,. 7
46.08
66.76
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