Document 3e2kd5Vqko0Xj45M0b3EvO1N3

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC206. Currentinformationindicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether,78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylene monooctylphenylether,and 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations ofimpurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: Not given. Type: Acute static GLP: No Year completed: 1976 Species: Pimephalespromelas Supplier:Not given. Analyticalmonitoring: Dissolvedoxygen,pH, and temperature. Exposure period: 96-hours Statisticamlethods: LC50 valuecalculateudsinggraphicinterpolation. Test fishage: Juveniles. Length and weight: Average length 50 mm Loading: Not given. Average weight 1.5g (wet) Pretreatment:None Test conditions: Dilutionwater: Carbon-filterweedllwater. Dilutionwater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:Not given. Concentrationsdosing rate:Once. Stabilitoyfthe testchemicalsolutions:Not given. Exposure vessels:Not given. Number of replicates:1 Number offishper replicateN:ot given. Number ofconcentrations:fiveplusa blankcontrol Water chemistryduringthestudy: Temperature range (0-96hours):21 -22 'C pH range(0-96hours): 7.0- 7.0mg/L(contreoxlposure) 6.7- 6.9 mg/L (1250 mg/L exposure)* Dissolved oxygen range (0-96hours): 5.0- 5.5 mg/L (controelxposure) 4.0 - 5.2 mg/L (1250 mg/L exposure)* 1250 mg/L (intermediatceoncentratione)xposure valuesused because the highestthreeconcentrationsresultedintotalmortalitbyy 72 hours. RESULTS Nominal concentrations: Blank control5,00, 1250, 2500, 3750, and 5000 mg/L. Element values: 96-hour LC50 = 1500 mg/L Element valuesbased on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206 96-hour LC50 forfatheadminnow was determined to be 1500 mg/L. Submitter: 3M Company, EnvironmeritalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordof methodology used and documentation. The sample puritywas notpropedy characterizedand thestudy lacksanalyticaclonfirmatioonfthe amount offluorochemical proportioninthe solution. REFERENCES This studywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, 1976. OTHER Last changed: 6/28/00. AQUATIC TOXICITY WOR SHEET 7)PP* Test Test Date Started 96 Hou F'tilgad Minnow Material Test Avg. weight Time Started 0 v Dilution Water Carbon Filtered Well w Date COmplete:d-.6---@ Plotted LC Siza* : Conc. 24 hrs % Survival D.0 H Control 7.'00 7.c) 6. @52 goo 48 hzs t Survival D.0 H too o 00 0 6- 72 hrs Survival 00 70H D.0 5,0 hrs Survival D.0 H 450 7.o @:o C) 70 7 (00 7., AV !!&O--Tcmverature 6,71. CC)) 0 1 ICSO Calculation + u -7 dp -Tf 4-10 20 0 40 so 6 70 80 9 100 Survival