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AR226-2774 FOR DU FONT USE ONLY Copies co: Haskell E I> du Itont de Nemours and Co., Inc. Laboratory for Toxicology and Industrial Elkton Road, P. 0. Box 50, Newark, Delaware 19711 Medicine HASKELL LABORATORY REPORT MO. 793-81 Material Tested Haskell No. 13.783---- Studies Initiated/Completed - 10/27/80 - 11/21/h------ 1/5/81 - 1/30/81 WHSK------ Material Submitted by MyBer Products Department Washington Works Rarkersburg, WV SUBACUTE INHALATION TOXICIT? STUDY IS RATS Sumaary; In 2 separate experiments, aale and female Crl;CD rats were exposed to imHBP----------------| in air. In each study, groups of 10 rats were exposed to design concentrations of either 0, 500, 5000, or 50,000 ppm for periods of 6 hours/day, 5 days/week for 2 weeks. The actual test concentrations for the male exposures were 541, 4750, and 46,300 ppm; female exposures were 473, 5140 and 46,800 ppm. Clinical observations of test rats during exposure were indistinguishable froa those of controls. Male and female rats in all test groups showed a normal rate of weight gain throughout the study. Clinical chemistry meaisureoents on rats exposed to 500 ppm showed no compound-related changes, following 10 exposures male rats exposed to 50,000 ppo tended to have significantly lower platelet counts and to excrete more fluoride in the urine than the controls. Female rats exposed to 50,000 ppm tended to excrete more fluofide and urobillnogen in the urine than the controls. Females exposed to 5000 and 50,000 ppm had significantly more oonocyces in the blood than the controls. observed 14 days poet-exposure. No compound-related changes were fltfB Pathologic exaoinatic showed no compound-related macroscopic or microscopic changes in any rats exposed to Company Sanitized. Does not contain TSCA CBI A comparison of organ Co body weight ratios between exposed vats and controls showed that following the 10th exposure, kidney weights were significantly higher than controls in both male and female rats exposed Co 50,000 ppn. Liver weights were significantly higher than controls in male rats exposed to 50,000 ppn. Fourteen days later these liver and kidney effects were still seen in Dale rats but had subsided in females. The significance of these effects is difficult to interpret since no specific microscopic lesions were observed in any of the racs exposed to----H I. Introductioni In 2 separate experiments, male and l^------UHUflHIIIBBRL^11 alr* ^he ^eCennlne the effectsofHIafCer repe concentrations were 0 ppa (Group I), 500 and 50,000 ppa (Group IV) female rats wer*. exposed purpose oC these studies II), _.nhalatlon exposure. , i .'Group 5000 ppa to was to Design (Group III) II. Procedure; A. Animal; Male and female Crl:CD rats sere housed 2/cage in 8" x 8" x 14** stainless steel cages and provided Ririna Certified Rodent Chow9 95002 and water ad llbitua. Rats were observed for general suitability for 1 week prior Co testing. 1^96 B. Exposure Protocol; In the first experiment:, male rats (weighing 230 Co 262 g), were exposed whole-body co vaporized hours/day, 5 days/week, for 2 weeks. In the second experiment, female racs (weighing 165 to 210 g) were exposed in the same manner* Control rats (males weighing 230 to 279 g; females weighing JL83 to 216 g) were simultaneously exposed to air only* All rats were weighed and observed dally (except weekends) through the exposure period and for 14 days post-exposure. C. Generation; Atmospheres were generated by syringe driving the liquid --KiTro^gh' a Spraying Systems nebulizer onto the heated surface (50C) of a round-bottom flask. Dilution air carried the resulting vapors into a 20 L glass exposure chamber. D. Analytical; flM^apors were analyzed on a Hewlett Packard 5710 Gas Chromacograph (GC) with a fijane ionization detector. The column used was a 6' x 1/4" glass coil packed with 10Z SE-30 on 60/80 mesh Chronosorb W-AW-DMCS. The column temperature waa 145C. Gas standards were prepared dally by air dilution of ^----^apors in calibrated glass bottles. Samples of chamber air were collected at half-hour intervals. Standards and samples were Injected into the GC by gastight syringe. Atmospheric concentrations were calculated from the standard curve and are expressed as the seain concentration for each 6-hour exposure. Company Sanitized. Does not contain TSCA CB1 1 2 E. Clinical Measurements ' : Clinical laboratory measurements were made on urine samples collected overnight following the 9th exposure and the 13th day of recovery. Analysis included a quantitative measure of the volume, oamolallty and pH, and semi-quantitative tests for occult blood, protein, sugar, bilirubin, acetone, and urobilinogen. Each specimen was noced for color and appearance and the sediment from pooled specimens examined microscopically. The concentration of fluoride and total fluoride were also measured* Blood samples were taken from the rats' tails after the 10th exposure and the 14th day of recovery for the measurement of the following: erythrocytes, hemoglobin, mean corpuscular volume, platelets, leukocytes and relative number of neutrophUls, lymphocytes eosicophils, monocytes and basophlls. The -hemat-oeri-^,- mean wrpuaeula*"heBoglobIir ana" niearTco rpuscular hemoglobin concentration were calculated from these data. Alkaline phosphatase, glutanic-pyruvic transaminase, glutamic-oxaloacetic transaminase, urea niCrogen, creatinine, total protein and cholesterol were also measured. 3 F* Pathology : After the 10th exposure, 5 rats from each group were selected at random and sacrificed for gross and hisCopathologlcal examination. Remaining rats were sacrificed on the 14th day of recovery for identical exemin.aei.OBS. Organs and tissues examined included the following: liver, kidneys, heart, spleen, bw..e marrow, thymus, brain, eyes, skin, ear, adrenals* pancreas, lungs, trachea, thyroid, parathyroid, lymph node, esophagus, stomach, small intestines, colon, and cecum. Also examined were castes and epididyaides in malea; ovaries, uterus, bladder, and vagina in females. G. Organ and Body Weight Analysis: At each sacrifice organ weights and organ to body weight ratios'were determined for the heart, liver, lungs, kidneys, spleen, testes (lales), ovaries (females) and thymus. These data are detailed in Appendices I and II. Walter B. Koniecki and John R. Barnes, "Subacute Inhalation of BBSESBBBBSBSSBBBBBiBBBlHCllliln"ical Laboratory Report, H-13,783, January 16, 1881. Walter B. Koniecki and John R. Bames," Subacute Inhalation of H-13,783, Mareh 20, 1981. Edwin F; Stula and William C. Krauss, "Pathology Report No. 19-81", H-13,783, June 17, 1981. CompanySanitized. 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V V 1 3- : "^ , = ^ * ' ;: -^ ' ? -y , P* ' j 3 0 m ^ i i s ff* <n i ? fv ^ i 1 i 1 * g . siljillllj ,' "--V^^t*f.*M'<fttifS* --ifl tt.~Sc~--^' - ? ; ?=?5SSS=Sg535==^S ^---\M:T^"*v'T"'r<v5''^*^--^J SS?^S;s=2 v .- c* tff s " e ?; ^ e mpikf^mr^^r^fM^ r-tN5--5?S'^5S"='5 <--S>JtM'.^1^ '^-- CV9-- V'tDV- SV>"Sb--- Bc'sB>e<aCs^lefl3s"olSs"'lsB iN1nri*e9ii>eviD<irnom"^i*ffi.t'--uiitff* 11 11 11 1 o ^< rtaf-ivanwaaoSasS*<or'-<e'pMs3--ves^" JV^ift'l^^Nifivrs & i - 1 iKa^i(S*Snint.>f':"nr-^?*--f<^too5-f>w^f*<P(-Nvsa^3 o<o<"'l^tfo3notof-cN5aro^ws . jSirNfNfeltS/i<^r^*mMirS*^"t<n " ~ ' f = ; ? ' ^> <% i 1 ; ^ ! s j J 1 i ! = S ^ "' w i 1 1 j 1 i i i !^ " - * 1 in ll ?<! ! rMCm3?'?*r--ft*i<r.'^"at*^tsCc^>tOa^toyn*ptan-i*rst-f eiofsl s.; t. s-evr--v*;--S-"-^owr^-s3ty^^oaw-*^^^o3- ;it ^e5 C"-i-^o*-.-r^>MiOrOttOy-<-tB^*n^arw^ttfs-f^^S^r , -sy x ^^wiyffMf*SBP^^--^0i"'^vwr^-^m="r^r<- "> 3 -- 5 '' ~ i >r i: ? ?! ^i -3 0.' ^O^tO^w.SiOr^m ; '^i*'--W^tTOO-t "' t." V ^ ff ifl l."l tft S ; < ^ y "^' i =l ; ".s-1"^"'"^"'^- 2 <- ^ M < " "^ ^ S = ft* 9>' "' ,2 ?-"^ ^ -- fM .5 = i s 1 Limr.^^ ^^r'.^^r^^off'"* : ^ ca ; S'^ .; . i ' ; 1 3^ -- f i .r.Mj 5"w ;--*<'*i i"r inTM>.-3^w * ^-^ i ' i * ^t??t'**-k*(icffi--^ft0tn<rcv r & / '"vvifftflv^Wtfl*."^ f. i : w : S" * e -: ; f t ; ' . ^ < i i ^ = [ . s; i ,, -~-,-.-,<,-.=-.= 7 a > : ^is^vipta^c^S ^ ; t c ^ i 1' E C.mpanysan.^.'"68"0' B. Clinical Observations: During exposure, clinical observaclons of cest rats were indistinguishable from chose of controls. II C. Clinical Chemistry; Clinical chemistry measurements on Group rats shoved no compound-related changes. Following 10 exposures, male Group IV rats tended to have significantly lower platelet counts and to excrete more fluorlde in the urine than the controls. Female Group IV rats tended to IIIexcrete more fluoride and urobilinogen in the urine than the controls. Group and Group IV females had significantly more monocytes In the blood than the controls. N6 compound-related changes were observed 14 days post-exposure. D. Pathology; Ho compound-related macroscopic or microscopic changes were observed- in any of the rats exposed t' jf----9 E. Body and Organ Weight Analysis; Male and female rats in all test groups showed a normal rate of weight gain throughout the study (Figures 1 and 2). Female rats in Group IV had significantly lower body weights than centrals during the exposure period, however, this was so prior to the start of the test and is not considered exposure-related. A comparison of organ to body weight ratios between exposed rats and controls showed a few differences. Following the 10th exposure, kidney weights were significantly highp than controls in both male and female Group IV rats; liver weights were significantly higher than controls in male Group IV rats only. These liver and kidney effects were still evident in males after 14 days but had subsided in females. The significance of these effects is difficult to interpret since no specific microscopic lesions were observed in any of the rats exposed Co ------ Company Sanitized. Does not contain TSCA CBl 5 - Work by; ff Hocerc T. Turner" Technician Report by; ,^- . - ------^U&^t >/f/lSyanne L. Ferenz -'/ Toxicologist ^/ Study Director Reviewed by: Approved by; OTT:jrg:wp:l5.i4 Pate is Report Mo. 793-81 jj, 1982 '^^d I-. Kennedy Section Supervisor, Acute Investigations Company Sanitized. Does not contain TSCA CBI 6 - FIGURE 1 s s s s s .1; jia&2 1 s^ li ef Company Sanitized. Does not contain TSCA CBl FIGURE 2. 1Q ~^ S u* I ' \ I ^ s 5 S tf - e Company Sanitized. Does not contain TSCA C APPENDIX I BODY WEIGHT SUMMARY Company Sanitized. Does not contain T<?CA ^RI <HALE) - BODY UEIBHT SUHHARY GROUP CONTROLS LOW (300 PPH) INT. (3(000 PPH) HI OH (30.000 PPH) F RATIO(l) L9D(2) DUHMETTO) UnS(4> INrERVAl.3 IN TEST CAYS 1. 2. 3. 249.3000 242.3000 249.0000 24S.2000 0.873 9.8247 11.9342 117.1167 ^34.3000 249.,0000 233.3000 3S0.7000 0.386 11.6802 14.2119 163.S306 262.3000 233.4000 2S9.2000 233.3000 1.101 12.1913 14.8339 180.3389 4. 26?.6000 237.2000 263.3000 238.6000 0.900 12.0293 14.6369 173.3806 285.2000 277.4000 282.8000 277.3000 0.808 12.6372 13.4006 194.3806 GROUP COHT ROLS LOU (300 PPH) IHT. i5(000 PPH) HIGH (30(000 PPH) F RATIO(l) L3It2) DUMNETTO) UH8(4) INTERVALS IN TEST DAYS a. 9. io. 293.7000 284.8000 289.8000 287.0000 0.609 14.1238 17.1831 242.0361 299,<j000 290.7000 296.3000 290.3000 0.763 14.9761 18.2222 272.1306 303.9000 293.1000 300.4000 293.9000 0.692 14.1802 17.3338 243.97SO 11. 109.4000 297.1000 303.7000 299.0000 1.306 13.0118 13.2656 273.4278 12. 303.0000 294.SOOO 304.7000 296.9000 0.937 IS.9331 19.3890 303.0972 GROUP CONrROLS LOU (300 PPM ) IMT. (3,000 P PM> HIOH (30>000 "PM) F RATXO(l) LSD<2) DUNNETT(3) UHS (4) INTERVALS IN TEST Oor; 13. 16. 336.6000 317.4000 322.6000 314.2000 1.493 24.2828 30.1238 328.0230 339.6000 319.6000 326.0000 316.8000 1.297 26.7418 33.1766 397.9230 343 .2000 323 .4000 334 .8000 323 .2000 1.341 24.0187 32.279s 37A.*000 IB. 3S- . -1.730 331 .0000 338 .4000 330 .2000 1.034 :"'.3897 34.;:94 423.4500 19. 3:6 .9000 338 . 000 ;46 .0000 334 .0000 1.::' :6.9"86 33.49;l 'o'.sooo .Company Sanitized. Does not contain TSCA CBI OROUP CONT ROL3 LOW :300 PPM) INT. S.OOO PPM) HIGH (5C.OOO PPM) F RATIO(l) LSD(2) OUNNETTO) UHS(4> INTERVALS IN TEST DAYS 22. 23. 24. 370.2000 350.0000 361.8000 350.8000 1.087 27.7262 34.3977 427.6500 376.2000 356.4000 368.8000 357.0000 0.935 29.808'; 36.9812 494.3000 384.4000 364. / 500 374.&000 36Z.6000 .078 29,0101 33.9907 4*8.17-30 389.6000 368.2000 378.0000 367.6000 1.01; 30.7992 38.2102 527.7000 26. 376.4000 362. :'..370.8000 356.6000 1.052 25.78-?? 31.9S" 369.125; (1) RATIO OF AHOM8- TO WITHIN-OROUP VARIATION--ONE-FACTOR ANALYSIS OF VARIANCE. (2) LEA8T SIGNIFICANT DIFFERENCE--GIVEN A SIGNIFICANT tALPHA-0.OS) F RATIOr ANY TMO MEANS DIFFERING BY nORE THAN THE LSD ARE SIGNIFICATLY DIFFERENT WITH A VARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF O.OS. (3) DUNNETT TEST--ANY TI<t.TMENT MEAN OIFFERINO FROM THE CONTROL MEAN BY lORE THAN THE DUNNETT STATISTIC IS SIGNIFICANTLY DIFFERENT PROM THE CONTROL MEAN UITH A VARIABLE-UIS2 FALSE POSITIVE (ALPHA) ERROR RATE OF O.OS. (4) UITHIN-OROUP HEAN SQUARE. I- SIGIU:1CANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY LSD. * SIOHIFICANTLY DIFFERENT (P<O.OSJ FROM CONTROL CROUP BY DUNNETT TEST AND LSD. .Company Sanitized. Does not contain TSCA CBI (FEMALE) - BODY UEIGHT SUMMARY OROUP CONTROLS LOW (300 PPH) IMT. (3000 PPH) HIQH <SO>000 PPH) F RATIO(l) L30<2) ODNNETTO) UN8C4) INTERVALS IN TEST DAYS 1. 2. 3. 197.1000 1911000 190.4000 134.4000* 2.437 9.3102 11.3716 109.7389 2w0.1000 194.8000 193.2000 188.3000f 1.972 9.7221 11.8294 114.6633 203.7000 197.8000 199.2000 189.9000* 2.928* 10.8839 13.2434 143.7933 4. 207.7000 198.4QOO 200.1000 192.7000* 2.723 10.'646 13.0979 140.3972 210.0000 203.3000 203.1000 194.6000* 3.102* 10.4713 12.7409 133.0389 OROUP CONTROLS LOU (300 PPH) INT. (3.000 PPH) HIGH (SOrOOO PPH) F RATIO(l) LS012) DUNNETT(3) UH8(4> INTERVALS IN TEST DAYS 9. 10. 220.3000 214.4000 212.9000 205.6000+ 1.944 12.4439 15.1411 187.8833 220.7000 213.4000 214.8000 206.2000* 2.341 11.2396 13.7002 1S3.B230 226 .8000 219 .3000 219 .3000 :11 .5000* 2.604 11.1129 13.5216 149.8417 11. 227 .2000 222 .0000 222 .2000 212.3000* 2.493 11.3282 13.7835 133.7028 12. '36.7000 .-.S.7000 222.3000 213.5000+ 1.771 12.008S 14.6113 174.9667 QROUP CONTROLS LOU (300 PPM) INT. (3.000 P PH> HIGH (30.000 PP*) F KATIOd) LSO<2) 3UMNETT(3) WHS(4) INTERVALS IN TEST DAYS 13. 16. 17. 240.6000 232.8000 239.6000 228.2000 1.381 14.9673 18.3690 1:4.6250 240.4000 326.8000 242.0000 228.0000 2.66; 14.7177 18.2391 1:0.5000 243 .0000 234 .4000 239 .4000 231 .8000 0.773 17.1017 21.2163 IA:.rooo 18. 24S . :000 236 .6000 243 .4000 ;36 .9000 0.693 11.^979 l'.8474 142.37SO 19. 208 .0000 :40 .4000 246 .4000 239 .2000 O.-'SO 14.7A30 19.3:1S '.:l.3;SC Company SamHzed. Does not contain TSCA CBI GROUP CONTfIOLS LOU <;SOO PPH) INT. <S>000 P PM HIGH (SO.000 PPM> F RATIO(l) LSD<2) DUNNETTO) UHS(4) INTERVALE IN TEST DAYS 22. 23. 24. 260. 2000 248. 2000 2S6. 2000 2SO. 6000 0.746 18.8290 23.3S97 197.2230 23S .6000 2SO .8000 2S9 .0000 2SO .8000 0.4-3 17.4000 21.SB68 168.42SO 257. 8000 2SO. BOOO 261. ^00 246. 2000 0.892 19.1473 23.7346 203.9500 35. 261. 2000 2S;. 0000 263. 2000 2S4. 4000 0.704 21.6987 26.9199 261,9250 24. ;61 .6000 2S4 .8000 26S .4000 2S7 .2000 0.484 20.2670 2S.1437 :z8.sooo (1) RATIO OF AMONG- TO UITHIN-GROUP VARIATION--ONE-FACTOR ANALYSIS OF VARIANCE. (3) LEAST SIGNIFICANT DIFFERENCE--8IVEN A SIGNIFICANT (ALPHAO.OS) F RATIO? ANY TUO NEANS DIFFERING BY MORE THAN THE LSD ARE SIGNIFICATLY DIFFERENT UITH A UARIABLS-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF O.OS. (3) THAN UITH DUNNETT TEST--ANY TF ATMENT H6AN DIFFERING FROM THE CONTROL MEAN BY MORE THE BUNNETT STATISTIC IS SIGNIFICANTLY DIFFERENT FROM THE CONTROL MEAN A VARXABLC-UI3E FALSE POSITTVE (ALPHA) ERROR RA OF O.OS. ;4' UITHIN-GROUP MEAN SQUARE. + SIGNIFICANTLY DIFFERENT (P<O.OS) FROM CONTROL 3ROUP BY LSD. * SIGNIFICANTLY DIFFERENT (P<O.OS> FROM CONTROL GROUP BY OUNNETT TEST AND LSB. Company Sanitized. Does not contain T8CA CB1 13 - APPENDIX II ORGAN & BODY WEIGHT DATA Company Sanitized. Does not contain TSCA CB1 n - (HALE) - ORGAN t BODY UEI8HT MEAN ABSOLUTE DATA RATS SACRIFICED AFTER 10 EXPOSURES GROUP FINAL WOT. HEART LUNGS LIVER CONSOLS LOU (500 PPH) XNT. (SiOOO PPH) HIGH (50.000 PPH) P RATIO<1) LSn<2> DUNHETTO) UH8(4) 298.4000 292.8000 303.8000 295.4000 .456 20.9699 26.0157 244.6250 1 .0100 .7400 1 .0760 1 .0920 2.517 .1312 .1628 .0096 1 .9280 1 .8320 1 .8240 1 .7360 .603 .3031 .3761 .0511 10.8380 10.7260 11.9740 13.3220* 4.0461 1.7388 2.1572 1.6819 SPLEEN .6940 .5940 .6480 .6360 .900 .1443 .1790 .0116 GROUP KIDNEY TESTIS THYHUS CONTROLS LOM <500 PPH) INT. (5000 PPH) HIGH (SOiOOO PPH) F RATIO(l) LSO(2) DUNNETT<3) UHS<4> 2.2520 2.2420 2.3760 2.6820* 3.3B1* .3347 .4153 .0623 2.9360 3.1300 2.8580 2.9940 1.401 .2907 .3606 .0470 .6660 .7600 .7530 .6960 .77S .1536 .1906 .0131 (1) Rtttio at orr- to ni.thin-<rou vrttion--on--fctor rrluis of vrrnc*. (2) tfniftCTit dlffrne--ivn unlficant (l^hfO.OS) F r*llo. Lft nw tMo n differing bu aor than th LSD r sianificantlu diffrnt file with vrl*bl-ui rocitiv (al^h*) rror rt at 0.05. (3) Dunrrtt ft--Ana tr**tirnt n diffnn fro* th con'rol in bw oiore than th Dunrftt tatlsti.c is ijnifientlw diffrnt fro t.r control an ulth vrl*bl-wi rl oitiv (jl^hf) rror rt of O.OS. (4) Mtthin-roup Hn Saur. T SinlflentlM diffrnt (P<0.05) fro control srouf b LSD. Sinificntlw 41ffTnt (P<0.03> fron control iroup b Ounritt tst nd LSO. Sinirie*ftt t thf 0.05 orobibilltii lvl. 15 - cwww" sanllh-- ^ "o. contein TSCA CBI (MALE) - ORGAN 1 BOOT WEIGHT MEAN RELATIVE DATA RATS SACRIFICED AFTER 10 EXPOSURES QROUP HEART L.UNCS LIVER SPLEEN COKTRQLS LOW (500 PPM) INT. 0.000 PPH) HIGH (S0>000 PPH) F RATIO(l) LSD(2) DUNNETT(3) UM8(4) .3391 .3213 .3343 .3693 '.484 7391 J483 .0009 .6436 .6296 .6007 S87S .587 .1036 .1283 .0060 i.6314 1.6604 3.9419 4.46204 7.108 .4330 .S372 .1043 S31S , ;030 . 2131 . 2319 1.02S .0423 .0524 .0010 KISMET .7^21 .7458 .-324 .9076* S.764X .0893 .1108 .0044 GROUP TESTIS THYMU8 CONTROLS LOU (500 PPH) INT. (5.000 PPH) HI8H (SOiOOO PPH) P RftTIO(l) LSD<2) DUHNETTO) UH8<4) .9833 1.0769 .9413 1.0136 1.787 .1273 .IS79 .0090 .2234 .2379 .2472 .2333 1,104 .0426 .0528 .0010 rvl, (1) Ratio at on<- to within-Tour variation--on-fetor analiris of vrl*nc. (2} jnM Lat ixniflc*nt diffTTict--Jlvn two n dirfrln bw or* thn th L 3Drn*rifitciilnnitfic(ilnithlw*04.0i5ff)TFnrtatior ulth vrlbl-wir f*L rocitiv (lh) rror rat* of O.OS. (3) Ounrrtt tt--An brftaant n diffTint froB th control i*n b or than tha Dunnrtt ttittic is Unifieantl'j difftrent fpoit tn egntrol 3an with (4) vrtabl-ui* rlr -oftitiv Uithin-roup Hn Sauar*. (alha) TPor raf af O.OS. + 3inificntlw dif'fTTit (P<0.035 froM control iroup b LSD. * Siniftc*ntlu 4xfrrnt (P<0.03) tra control Jroup bM 3<jnntt tt and L30. t Srniricjnt t th O.OS rrobbiXit CompanySanitteed. Does not contain TSCA CBI (MALE) - ORGAN 1 BODY UEIGHT HEAN ABSOLUTE DATA RATS SACRIFICED AFTER 14-DAY OBSERVATION BROUP FINAL WOT. HEART LUNOS LIVER CONiROLS LOW (300 PPM) INT. (S>000 PPM) HIGH (50,000 PPH) F RATIOd) LSD<2> DUNHETTO) UHS(4> 376.4000 362.2000 370.8000 336.6000 1.0S2 2S.7S92 31.9575 349.1230 1 .2140 1 .1420 1 .1380 1 .1720 .420 .1420 .1772 .0113 1 .9440 1 .9080 i .1440 1 .9290 2.467 .2093 2S97 .0244 14 .3080 13 .0680 15 .2000 13 .2600 2.298 2.02S8 2.5133 2.2831 SPLEEN .6:80 .7:20 .70:0 .6720 .434 .1316 .1633 .0096 OROUP KIDNEY TCSTIS THYHUS CONTROLS LOM 000 PPH) INT. <S*000 PPH> HIGH (S0>000 PPH) F RATIO(l) LSD(2) DUMNETTO) HHS(4) 2.7760 2.3740 2.9380 3.0220 2.493 .3747 .4649 .0781 3.3140 3.2940 3.2720 3.1740 .288 .3470 .4304 .0670 .7760 .7340 .8060 .8660 .S33 .1958 .2429 .0213 Lft (1) Ratio of on*- to uithimrou^ vnation--or>-fetor anlur of varrnc*. (2) inificnt diftTnc--lun nnlf'tcnt (lptfO.05) F r*tlo, nw IMO n dirfrin bw or than th LSD r* nnifientl iliffTnt with (3) D vrl unfft b t l-wi* tt--An f t l r f r t powl nt t iw ( n Iptr diff ) T i rro na r fr ra o t * th o e f O co .O nt S. ro l *an toy aor* thar, th Dunnitt tstiittc ! iniricintlu 41fftrnt fro th control nrjn with <4) vrfblc-wif fair poxitiv Uithin-arou^ Hn Saur. (lph) rror rt of 0.05. + Sinificntltt dirfrnt (P<O.OS> fro control aroup b LSD. * 3Hr>iflcntIv dlffernt (P/0.05) fro* control irouc bw Dijnnett tst ind LSD. CompanySanllhed. Does not QonWn T8@^ e^ OROUP (MALE) - ORGAN 1 BODY UEIGHT MEAN RELATIVE 3AT^ HATS SACRIFICED AFTER H-Ofi; 1BSERVATION HEART LUNOS L:VER SPLEEN CONTROLS LOW (300 PPH) INT. (3.000 PPH) HIGH (S0>000 PPH) P RATIO(l) L3D(2> OUNNETT<3) UH3(4> .3234 .3154 .3116 .3286 .473 .0334 .0413 .9004 .3179 .3278 S782+ .3400 2.123 .0343 .0673 .0016 3.1098 3.6062 4.0969 4.2715+ 3.7l9 .4602 .5709 .1179 17S1 .1991 .1393 .1887 .758 .0340 .0421 .0006 K:CNEY .7401 .71:3 ."908 .34334- 3.143 .1013 .1237 .0037 CROUP TEST IS THYMUS CONTROLS LOW (SOO PPH) INT. <3>000 PPH) HIGH (30*000 PPH) F RATIO(l) LSO(2> OUNMETTO) UH8(4) .3841 .9093 .8829 .8933 .103 .1133 .1408 .0072 .2032 .2082 .2176 .2420 1.148 .0467 .0579 .0012 tftirnt (1) (2) R*tio of on*- to uithin-jrou^ vritlon--on-fctor nalwr of vrrnc*. Lt inifient cliffrnc--ivn sinificnt dl^hj'O.OS) F ratio* nw txo n diffTina b with vrlbl-wlf flr or* than th LSO r sisnKicTitlu liffifnt ^osifciv Clrh> rrop rf of 0.03. (3) Dunntt t.t--Anw an diffrin froa th control n b or* than ih Dunnatt ttl*tic f llnificantlw >Jiffrnt froB th control *n with (4) vrrblc-Mif rl* potitiv Uithin-aroup Hn SQUT*. Cipht) rror rit* of O.OS. + 3ijnificntlu diffrnt <P<0,03) froa control JPOUP b LSD. * Siintficintlw dlffrnt (P<0.05) fro* eontnl arou ?< Dunntt t*t nd LSD. * Significant t th O.OS probabilitu l*vi. Company Sanitized. Does not contain TSCA CBS (FEMALE) - ORGAN S BODY UEICHT MEAN ACSOLUTE DATA RATS SACRIFICED AFTER 10 EXPOSURES GROUP FINAL U8T. HEART LUNGS LIVER CONTROLS LOU (300 PPH) INT. (S.OOO PPH) HIOH (50.000 PPH) F RATIO(l) L3D(2) OUNNETTO) UH6(4) 222.0000 223.2000 210.4000 208.8000 2.432 14.3132 19.0054 117.1750 .7780 .8640 .8260 .7560 1.772 .1091 .1354 .0066 1.5720 1.5780 1.4420 1.3900 1.678 .2181 .2706 .0265 9.0640 8.5200 8.5640 3.3500 .731 .8966 1.1124 .4473 SPLEEN .6040 .3110 .50:0 5<2i 1.277 .1165 .1443 .0075 GROUP KIDNEY THYHUS CONTROLS LOU (500 PPH) INT. (5(000 PPH) HIOH (50r000 PPH) P RATIO(l) LSD(2) DUNNETTO) MH8(4) 1.7880 1.8460 1.8520 1.9180 .801 .1782 .2210 .0177 .6020 .7160 .5740 .6740 1.220 .1767 .2193 .0174 (1) (2) Ratio Lt of aonx- to ulthln-aroijr vrition--on-factor rrlxti* rmficint <liffTnc--ivn isniflc*nt (l^hO.OS) of F vrrnc*. ratio* nw two n alffTina b or* then th LSD r iinlficnU diffrnt uith (3) O uvnrrlrattblttutt---ulAcnyfatirrwtpironstitivan(ldhiff>Tinrrtorfforii tthofco0.n0t5ro. l irn b or* thn th Dunntt ctjtittic x i*niflc*ntlw ijtfferant froa th control n with (4) H tvthrlnl*-fblrlou-pwHifanfiSleaupr. itiv (ll^ha) rror rt of 0.35. Slnific*ntlw diffrnt (P<0.05) fro* control rojp b LSD. SfnificntlM 41ffarnt (P<0.05) froa control sroijp bu Dunntt test no LSD. - Company Sanitized. Does-not contain TSCA CBa (FEMALE) - ORQAN S BOOT UEIOHT 1EAM RELATIVE PAT* RATS SACRIFICED AFTER 14-Ofl' 3B3SRUriON GROUP HEART 1 '.""; i-iyER SPLEEM CONTROLS 1 3N (300 PPH) INT. (3i000 PPH) HIOH (SOiOOO PPH F RATZO(l) (.30(2) DUNNETTO) UHS(4) .3346 .33-1 .3704 .3333 .899 .0443 .0574 .0012 .6989 .6265+ .6370 .6394 2.173 .0404 .0749 .0030 3.6533 3.7989 3.8730 3.8990 .407 .5015 .6:21 .1399 .;3;6 .2223 .1939 .2332 1.722 .0469 .0582 .0012 M3NEY .7373 791A S087 .7702 .658 .0841 .1044 .0039 GROUP OUARIES THYMUS CONTROLS LOU (300 PPH) INT. 0.000 PPH) HIGH (SOrOOO PPH) P RAridd) 1.30(2) DUNNETTO) UH9(4) .0602 .0636 .0634 .0393 .167 .016. .0201 .0001 .2647 .3071 .2605 .2611 1.173 .0625 .0776 .0023 (1) Rtlo of ron- to ulthin-trour s>rnt.ion--on-f actor anli of vrunc*. (2) ijniftcant 41ff*pne--tvn iianiftctnt (lhO.OS> F rtia> Lft fw tKO n aittirirn b of than the LSO r ianiricntl diffepant ulth <3) vrlbl*-ul rlf oitiv (alpha) rror ri^ of O.OS. OunnBtt tt--Ar> trafnt n dlfrnris fpo tn control aen ?-< or thn th Dijnrrtt sfttitic n inificntl liffvrerit from th eontpol i>an f*lf ulth vrrbl-wlc oacltiv (al^h*) rror rt of O.OS. (4) Utthin-raui> Hn Saujr*. + Sfniftctnilw diffrnt <P-"O.OS) froi control iro'jp &< LSO. * Sfnificintiv 4iffrnt (P'0.03) fpoa control iraup 5a Ounntt *s^ ntf LSD. .Company Sanitized. Does not contain TSCA CBI OROUP (FEMALE) - ORGAN 1 BODY UEI6HT MEAN RELATIVE DATA HfTS 9aCRI:--IC0 AFTER 11 EXPOSURES HEART LUNOS LIVER SPLEEH CONTrOLS LOW (SOO PPH) IHT. (S.OOO PPM HIGH <SO>000 PPM ( RftTIO(l) L3D(2) OUHNETTO) UH8(4) .3514 .3877 .3914 .3626 1.464 .0480 .OS96 .0013 .7C34 .7071 .^863 .168 .423 .0869 .1078 .0042 4 .->831 2 .8187 4 06S7 4 .2426 2.619 .3243 .4023 .0585 .2719 .2:01 .2384 259S 1.436 .0479 .0394 .0013 KI3NEY .3074 .3274 .3810 .9136* 3.908* .0768 .0953 .0033 OROUP THYHUS CONTROLS LOU (500 PPH) INT. (3000 PPH) HIGH (SOf.OOO PPM) F RATIO(l) LSDC) DUMMETTC3) UH3; .2699 .3212 .2727 .3233 1.250 .0792 .0982 .0033 (1) Ratio of on- la uithln-trour vrltion--on-fctor nljsi* of vjrijnc*. <2) Lt Untfiont ()iffPnc--alvn iHnificant (l)h-O.OS) F ratio *nw two n 4iffrlh bw or* than th LSD *r IxnificintiM 4irrrnt Mith (3) D vr unr i r tt bl-u tt-- ir An w f at ri r po tB ct nt t iv C n alr'ha) diffc r trr lna o r f r ori f t r o r f c O o .O nt S. ro l fan py <*or than &h Dunrctt ttiitic r xjnxflc*ntl dxf^Tint f'oi ^'r control inain with (4) vPi*bl-wi fl poiitiv Ulthin-aroup Hn Sajr. (l^h) rror ri* of 0.0:. 4' Silniflontlx dlffTnt <P<0.05) fro* control TOUP b LSD. * Sijniflcntl 4iffT*nt (P<O.OS) froB control ro'j &u Punn".t *n. *nd LSD. < Sl*nificnt at thf O.OS rrobfbilitv lvl. Company Sanitized. Does not contain TSCA CBI GROUP <FEMALE) ORGAN ( BODY HEIGHT MEAN ABSOLUTE DATA RATS SACRIFICED AFTER 14-OAY OBSERV--I3N FINAL HOT. HEART LUNGS L:VER CONTROLS LOW 000 PPM) IMT. <S000 PPM) HIGH (SO.OOO PPM) f RATIO(l) L8D(2) OUMNETT(3) MHS(4) 261.6000 234.8000 263.4000 2S7.2000 .484 20.2670 25.1437 22B.SOOO .9740 .9040 .9840 .9100 .880 .1494 .1853 .0124 1.8260 l.:9b0* 1.7420 1.7020 1.797 .2136 .2650 .0234 9.5800 9.6800 10.2700 10.0040 .363 1.3669 1.9439 1.3659 SPLEEN 6120 S740 .5140 ol40 1.003 .1413 17S3 .0111 GROUP KIDNEY OVARIES THTMUS CONTROLS LOU 000 PPM) INT. (S000 PPM) HIOH (S0>000 PPfO F RATICX1) L3D(2) DUNNETTO) WHS(4) 1.9790 2.0180 r:.1460 1.9760 .993 .2408 .2988 .0323 .1360 .1620 .1680 .1520 .302 .0382 .0473 .0008 .7000 .7820 .6880 .6720 .647 .1827 .2266 .0186 <l> Ratio of onj- to Mith^n-sroup vrltion--orr-fctor anln*r of vrir>e. (2) ixniriccnt <lt/frnc--atvn ifniricint Cl^hfO.OS) F ntioi Lft nw two n dIffTirft b or thn th LSO r lrnficTitl dIffTTit f*lr utth vprbl-wr poitiv <lh) trror rt at O.OS. (3> Ounnitt tft--Ariw t.rfnt n diffrirn fro* th control in b norc ftrtic than th Di.mrrtt it tilnlflcintlw diffcint froR th control on with (4) Ulvthrinr-bToru-up tHr*nfSI*auapr*o. fitiv (llrh*) trror rt of O.OS. + Sinificntl diffTint (P<O.OS fro control sroup t>w LSI). * &kniflentlw and LSI). 4iffrnt (P-'O.OS fron control rou Bw Ounnett fst Company Sanitized. Does not contain TSCA CBl