Document 3RQ7Ve7dpLz5jnGYmXMYwzn3

M za.6 - 0 7 9 7 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE___________________________________________ Identity: Mixture containing Perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206A. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was cc 785-22. The test sample is FC-206A. Current information indicates it is a mixture of 0.92% PFOS, 18.75% diethylene glycol butyl ether, 75.44% water, 1.83% Hydroxy foamer, 1.75% sodium octyl sulfate, 1.2% poloxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: Not given. Type: Acute continuous flow GLP: No Year completed: 1979 Species: Pimephales promelas Supplier: Commenai supplier in Brady, NE Analytical monitoring: Temperature, pH, and DO. Exposure period: 96-hours Statistical methods: LC50value calculated using probit computer program. Test fish age: Juveniles Length and weight: Average length = 41 mm Average weight = 0.64 g (wet) Loading: 0.64 g/L Pretreatment: None Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Hardness: 236 mg/L as CaC03 Alkalinity: 221 mg/L as CaC03 Conductivity: 430 pmhos/cm Lighting: Not given. Stock and test solution preparation: A primary stock solution was prepared at 210,000 mg/L. General dilution factor of 70, equivalent to 3,000 mg/L. The primary stock was proportionally diluted with dilution water for the additional concentrations: dilution series 100%, 60%, 40%, 20%, 10%, and 0% . 001609 Flow-through rate: Approximately 4.5 volume additions of test solution every 24 hours. Concentrations dosing rate: Continuous Stability of the test chemical solutions: Not noted. Exposure vessels: Glass tanks (36x21x24 cm) containing 16 L of test solution. Number of replicates: 1 Number of fish per replicate: 16 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.9 - 8.0 (control exposure) 7.6 - 8.0 (3000 mg/L exposure) Temperature range (0-96 hours): 18-21 C Dissolved oxygen range (0-96 hours): 7.0 - 9.3 mg/L (control exposure) 4.5 - 9.3 mg/L (3000 mg/L exposure) RESULTS____________________________________________________ Nominal concentrations: Blank control, 300, 600, 1200, 1800, and 3000 mg/L Element values: 96-hour LC50 = >3000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be >3000 mg/L, the highest concentration tested. This concentration caused a 12.5% mortality. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 3. Testing lacks record of methodology used. There is a lack of characterization of the test sample and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 001610 REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 4970S, 1979. OTHER_____________________________________________________ Last changed: 6/26/00 001611 ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SICET LX O S Typ Test C .T L v4 Ma te r ia l Tested T " C - 2 . & A -Lot - Test O rg a n ise *uW.fit ^ ^ --------- S t MftV,. Pe te Becd. - 7 - 7 ? Batch No. g Avg. N t. . ,2 . frees A v*. Site 4.1* t & No. Orgmisaa pe r dose ______ ^ _______ T e st S o lu tio n Voluee 16 L i tO T s/A ll-G la ss T n k Tank D ie n t ions : 36x21 ce and 2A ce deep T in e S ta rte d Date S ta rte d - /7 ~7 9 Date C eopleted 7 - 2 / - 79 D ilu tio n Water C a rb o n -filte re d .w e ll w a te r { Teae. C .. D.O. ^ . 3 ppe. pH g . o ) v? In v e s tig a to r L C ^ (9S I Coefidence L ia its ) k n it. Tani|v Cone. No. aa/1 .'^ C o n tro l 1 Soc? 2 oo 3 tzoo <1 t o o r 3 , coo T tz r Aeount ~ ----- ^ wours T o tal D.O. No. Dead P ppn O 7.9 .5 4.e<> 9,6*0 l t 2.oo 2.2, Too 4 ,coo 0 7? 1 7.6 * .0 0 9 6 7.7 1 1-6 7 .7 I + 7 .5 -- ---- io u rs T T 1 w T o ta l D.O. T o ta l .0. No.Dead pH P M Ho.Oead & PPf o 7.9 7 ? O 7.* 2 ? 0 7 .? 7 .0 \ 7. S .r 0 7.7 7. 1 7" 7 0 7-6 0 7.i \ n.g s :4 , i ; 7/1 r . r i n.G 4 . ? 7 n i t 4 * "T8H T otal 5 S F* D. No.Dead s C> 0 7 O 7; .2 1 7/ f o 7.1 f 1 77 5 *2. 4 Test Tea*. C. S ignature 6 Date o 2od V v .\ Ww 1- /S-7? *2. rrj. tu-ui 9 - /?~9$ Z I< 1 , ?P C n--zo-79 1 - 2 ) --?< Coeeents i0 S>y.SisVm , WAfr-.V S lV n \ WB.WL j S V t* 2'.jkLz pfroeJAV,,. fKtxtftreJfStCiai a la a L . ....WW itlifll-_,._)_/2_t_J_c_r_._.L_k_y_,_.k_V.v.l.,..., iL^.>nftVAfi&fc .St? ^ .e.fr.-i-B---W- A 4 ^ - U o ^ r J^V-voc^c evpoS'^v-R. S\a.<KxCvV.A lowfiivt w.ot>ir^\;vn E ffe c ts ot)\.er than M o rta lity EC, \ V>^K. rw^,i i o wwy J WW" J g f U . - - ---------- ----- --- In it. Cone. T o ta l No. A ffe c te d g/1 C ontrol Syeptoes o r Reactions 24-H r. / ---------TO------ 4S H r. / f>J 4- 72-H r. . 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