Document 3QyYXmrg2jEa2X8JEM71VqwKJ

> PROPOSAL TO THE NEW YORK STATE HEALTH RESEARCH COUNCIL TITLE: The Environmental Health Implications of Aerobic and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) in the Hudson Estuary PRINCIPAL INVESTIGATOR: Dr. Chun C. Lee (SSN 521-52-6704) INSTITUTION: New York University Medical Center Institute of Environmental Medicine Laboratory for Environmental Studies 550 First Avenue New York, New York 10016 DURATION OF PROPOSED WORK: January 1, 1979 through December 31, 1979 TOTAL AMOUNT REQUESTED: $31,080 Chun'Chi Lei ----------------------- Research Scientist Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 --///Ls p HefrlTEisenbud-------------------Associate Director Institute 'of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 ///>! Norton Nelson, Director Institute of Environmental Medicine New York University Medical Center 550 First Avenue New York, New York 10016 ' Thomas_A- Fitzgerald, Director Office of Grants Afalalstratlon and Institutional Stadias 550 First Avenue New York, New York 10016 HONS 009124 NEW YORK STATE HEALTH RESEARCH COUNCIL GRANT APPLICATION PRIORITY AREA i 7 LAST NAME Lee SECTION I SOCIAL SECURITY i 521-52-6704 ___________ TO BE COMPLETeQ 8Y PRINCIPAL INVESTIGATOR '' ' 1. TITLE OF PROPOSAL The Environmental; 2. G. DQARIMEMT, SERVICE, LABORATORY Health' Implications of Aerobic and An|- OR EQUIVALENT Lab. for Environments aerobic Decomposition.of Polychlorinated T. PRINCIPAL INVESTIGATOR Biphenyls (PCBs) in the Hudson Estuarv2. H. T. "TC. NAME (Luc^, First, Initial) Studies, Inst, of Environmental Medicine, NYU Medical Center MAJOR SUBDIVISION Medical Center Lee, Chun C. 3. DATES OF PROPOSED PROJECT PERIOD (THIS T. r title op position Research Scientist and Adjunct Assistant Professor APPLICATION) . Proa Jan. 1, 17 9Thrqugh Dec. 31, 1979 TOTAL DIRECT 5. DIRECT COSTS COSTS FOR PERIOD REQUESTED FOR FIRST 2. C. MAILING ADDRESS (Street, City, IN ITEM 3 12-MONTE PERIOD P.Q. Box 817 Tuxedo NY 10987 State, Zip Code) -31i.SJS0 S31.080______________ 6. PERFORMANCE SITE TIT A. Primary Site A.J. Lanza Labs P*h.D. S. SOCIAL SECURITY So. 521-52-6704 NEW TORE STAIR SENATORIAL DISTRICT NUMBER 26 2. F. TELEPHONE NUMBER AC (914 )35I-4229 EXT. C. NEW TORS STATE ASSEMBLY DISTRICT NUMBER ' 65 7. RESEARCH INVOLVING HUMAN SUBJECTS A. _X__No B.____Tee-Approved:____ Data C. Tea-Pending Review TO BE COMPLETED BT RESPONSIBLE ADMINTSTRAITVE AUTHORITY 8. APPLICANT ORGANIZATIONS (S) [10. NAME, TITLE, ADDRESS, AND TELEPHONE NO. Institute of Environmental Medicine OS OFFICIAL IN BUSINESS OFFICE WHO NYU Medical Center SHOULD ALSO BE NOTIFIED IF AN AWARD IS MADE. 550 1st Ave., New York NY 10016 IRS * 135562309N__________________________ John N. Bellow, Vice President for Finance TELEPHONE NUMBER 212 679-3200 ext. 3611 9. NAME, TITLE, AND TELEPHONE NO. OF 11. IDENTIFY ORGANIZATIONAL COMPONENT TO OFFICIAL (S) SIGNING FOR APPLICANT ORGANIZATION T-A. Fitzgerald, Director RECEIVE CREDIT FOR INhlilUllONAI GRANT (SEE INSTRDCTIQNS) a, 01 New York University Medical Center rvv-TTTriTTntt awn arrmriMr-g. we, THE UNDERSIGNED, CERTIFY TSAI THE STATEMENTS BERETS ARE TRUE AND COMPLETE TO THE BEST OP OUR ES0WLSDG3 AND ACCEPT, AS TO ANY GRANT AWARDED, THE OBLIGATION TO COMPLY WITH NTS HEALTH RESEARCH COUNCIL TERMS AND CONDITIONS IN EFFECT AT THE TIME OF THE AWARD. SIGNATURES (SIGNATURES REQUIRED ON EACH A. SIGNATURE OF PERSONI NAMED IS ITEM 2.A. C. /c-xr-T? COPT. USE DARK INK. "PER" SIGNATURES NOT ACCEPTABLE.) Be SIGKAXUHS_QE_221SCN NAMES IS U5K 9. DAIS trr-z? * (SOTS: Principal investigator oust also profess and sign professional assurance at che end of research plan) HONS Q091d5 'I Section X RESEARCH OBJECTIVES Priority Are* 7 ) Last Hams Lee Social Security t 521-52-6704 NAME AND ADDRESS OF APPLICANT ORGANIZATION Mew York University Medical Center, Inst, of Environmental Medicin* 550 First Avenue, New York, Hew York 10016 NAME, SOCIAL SECURITY NUMBER, OFFICIAL TITLE. AND DEPARTMENT OF ALL PROFESSIONAL PERSONNEL ENGAGED ON PROJECT, BEGINNING WITH PRINCIPAL INVESTIGATOR Chun C. Lee, Ph.D., 521-52-6704, Research Scientist and Adjunct Assistant professor. Laboratory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center (914)351-5419 Theo. J. Kneip, Ph.D., 031-36-5155, Deputy Director, Laboratory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center ' Joseph O'Connor, Ph.D., 011-34-2794, Assistant Director, Labora tory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center TITLE OF PROJECT: THE ENVIRONMENTAL HEALTH IMPLICATIONS OP AEROBIC AND ANAEROBIC DECOMPOSITION OF POLYCHLORINATED BIPHENYLS (PCBS) IN THE HUDSON ESTUARY I . It is known that PCBs recovered from environmental samples (e.g., water, sediments and organisms) are different from commercially .produced products by having an increased abundance of isomers of high'chlorine content; isomers of low chlorine content are readily metabolized by organisms at lower trophic levels, or are otherwise degraded in the environment. It is also known that PCB isomers containing 50t or more chlorine are less active, biologi cally, than the lower chlorinated isomers, hence those isomers having high chlorine content may be less toxic. Consequently, the health risk from environmental accumulations of PCBs may change with time because of differences in the degradation rate. This study is proposed to investigate the mode and rates of PCB degradation (Aroclor 1221, 1016, 1242 and 1254) by aquatic and estuarine fungi and anaerobic bacteria, and to assay the relative toxicity of the PCB mixture remaining after micro bial action against some key Hudson River organisms. MONS 009126 P 49 MflJICT COtfraOLI Priority Ares #__~J_________________ Lut Hm ke____________________ Social Security f 521-52-6704 I HBC # (Office uae pair) | Type of Application: Total Direct Coats Requested: * Sew Pirac Tear S S31.080 . "-! of BBC #____________ Total 9 S.A.___________ * Date Submitted November 1. 1978 Principal Inveatlaator chun C. Lee, Ph.D. Applicant Inatlttttion New York Pniverslty Medical Center laaaarcb Grant Manater: Institute of Environmental Medicine. NYU Med Center Ira S. Goodman - Assistant Director, Office of Grants n< <-ra+-4rm c m-t7a--nnn X2233 naaw phone Title of Proposal: The Environmental. Health Implications, of Aerobic and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) in the Hudson Estuary DO WOT WRITS BELOW THIS LOBE Coaolaeeness Validation (1) Proper SimaCuraa <2> Patent/Copyrijht Acreement (BBC-23) (3) Protection of Htimon Sttblocto (BRC-l) (*) Protection of Laboratory Animal a (Latter) (3) Principal Investigator Aasnraace (Stateaant and Signatures) (6) Progress Kapoxt (Renewal) (7) Invention Report (HRC-11) (8) annual Invention Statement (BKC-22) 5 1 Data ftacolrod . Data HONS 009127 nsection U psiqxitt im #__ Luc HaasLee nXTUMSSD COUMDHICmaB Social Security # 521-52-6704 c nrnrtm BUDGET FOX FOST 12-MQKTH FOtZOD AMOOXI H0UTL51U) O) pexjcnnel ZZTLZ OF POSITION Z or TBS OX EFFOXT SALAMI IXQKS saix z total 20.5r^o 8/31/79 9 ilL afters 9/1/79 f . P. Lee Principal Investigator___2Q%_ 4.486 974 5.460 ill.M. O'Connor T TWA TWA Technical Advisor Technical Advisor Secretarial HeId 5 5% 20* 100% N.C. N.C. 2,407 12.000 m- -0- _=fl=__ .. 2.939, 14.606 (2) cosscmirr cosxs_ U> Subtotal *8-H97 -lm 122.996 (2) Subcscal SDCIPlgaiT v.^.hl. hV-r , .5,00-------------------------------------------------------------- afl-. (3) Subtotal f 500 (*) suFFLIZI'hemlcals. reagents, media supplies. Boutin# glassware and solvents. 9 Miscellaneous supplies . (4). - s Subtotal (3) TXA7TL f -n nm rtnniea-M n meoH im and .-revel <-n rnnnl<- th relet-n/1 '-! i-V (3) Subtotal f 1.000 1,000 750 250 3,000 400 ann (S) rszzzsr costs. c 50Q * 3-PQQ 400 (6) Subtotal T~ O) TENSES (DETAIL) Overhead (154 nf 1____4il84 ~ Subtotal (8) ALL D33ZCT COSTS.................. ...................................................TOTAL S 4.184" 7*** ? MONS 009128 1___-0---- rS 4.184 S31.080 2 l) szcrrcar u IDDCET ESTIMATES POE 701012 TEAM DISZCX COSTS OHLT (Omit Cues)* ) Priority Area # 7 Last SameLee Social Security # 521-52-g7na Description First Tsar (Same As. Detailed Budget on Fate 3 Second Tear a) Personnel Coats, laeladlag fringe benefits ( Consultant Costa (Include fees, travel, ate.) $22,996 -0- NA (3) Equipment <*> Supplies (3) Travel () Patient Coats (7) Other Expanses (overhead 15* of total) 500 3.000 ___1M. ^__ JulM. TOTAL DIRECT COSTS 31.080 I Ma J2MARXS: Justify all coats for the first year for uhleh the need osy not he obrlous. For future years. Justify equipment coses, as well as any significant change la any other category. (Dae continuation page If needed.) Five hundred dollars is requested for the purchase of a small variable shaker assembly for the growth of fungal and bacterial cultures. This piece of equipment is not available at the Institute. BOTZi Completion of theee eolumne doea net obligate the STS Health Raaaareh reisirfT, to future funding. .An application for ranees! rust be filed each year. Page A HONS 009129 SECTION IX ) Priority Area I Social Security # 521-52-6704 grant RESEARCH SUPPORT list all research support for each individual including regueses now bolng considered, aa soil aa any proposals balng plannsd, rsgsrdlsss of ravalanca to this application. Ineluda also cnrrsst or ponding contracts, fellowship swards, research casaor progran swards, training grants, rogsrdlsss of Cho sourca of support. Ineluda tltla of project, funding sourca. aaounc of support, and investigator's tins eonaltneac. List grant nambor(s) for all currant support. Support for Lee ' {yrr.*.! Mew York State DEC - Monitoring of PCBs in the Hudson River June 1, 1978 - May 31, 1979 $44,000. 20 % of time. ' Mew York University Medical Center Institutional - The Anaerobic Degradation of PCBs by Aquatic and Estuarine Bacteria. July 1978 - March 1979. $5,000, no salaries. Ponding; New York State ERDA - Power Plant Simulator Studies December 1, 1978 - November 30, 1979 $90,000. 10* of time Planned: u. S. NOAA-Sea Grant - Comparative Toxicity of of Environmental PCBs vs. Commercial Preparations of PCBs in Hudson River Food Fish. Approximately $75,000. U.S. EPA - A Proposal to Devise Clean Up procedures for Labor atory generated wastes. Approximately $10,000. Page 5 HONS 009130 2 ) BIOGRAPHICAL SKETCH Priority Area t___ Jet Km Lee 4 Social Security t 521-52-6704 (Give the following Information for all professional peraonnel beginning with the Principal Inveatigator. Dae continuation pages and follow the aame general format for each person.) *' NAME TITLE B1RTHDAIE (Mo.,Day, Tr.) Chun C. Lee, Ph.D. Research Scientist and 08/31/39 _____________________________________________Adjunct Assist. Prof.________ PLACE OF BIRTH (City, State, Country) Hong Kong Present Nationality (If non- O.S. citizen. Indicate kind of visa and expiration date) Sax Male Pamela EDUCATION (Begin with baccalaureate training and include postdoctoralT . TEAR SCIENTIFIC INSTITUTION AND LOCATIONDEGREE CONFERREDFTRTD Colo. State U. Ft. Collins, Colo. B.S. 1962 Zoology U. of Miami Coral Gables, Fla. PH.D. 1969 Marine Science HONORS Achievement Award in Biological Science, Colo. State U. 1962. NDSA Fellow 1962-1965, 0. MAJOR RESEARCH INTEREST Environmental Pollution of Miami. ROLE IN PROPOSED PROJECT t Project Director RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, Jlat training and experience relevant to area of project. List all relevant publications.) See Attached Curriculum Vitae Page 6A MONS 009131 r-jorlty Area #___ 7 BIOGRAPHICAL SKETCH Last Ham* **ee > Social Security # 521-52-6704 (Siva tha following information for all profeaslonal paraonnal beginning with tha Principal Investigator. Use continuation pages snd follow the same general format for each person.) - NAME TITLE aiRIHDAIE Theo. J. Kneip, Ph.D. Dep. Dir. Laboratory for Environmental Studies (Mo..Day, Tr.) 12/20/26 PLACE OF BIRTH (City, State, Country) Present Nationality (If non- Sax V.S. cltlxan, indicate kind Male St. Paul, Minnesota, USA of vlaa and expiration date) ___Female EDUCATION (Basin with baccalaureate training and include postdoctoral) TEAR SCIENTIFIC INSTITUTION AMD LOCATION________DECREE CONFERRED________FIRM) University of University of University of Minnesota Minnesota Illinois B.S. M.S. Ph.D. 1950 1952 1954 Chemistry Chemistry Chemistry BOMORS Phi Lambda Upsilon, 1950 - Sigma Xi, 1954 MAJOR RESEARCH INTEREST Environmental Pollution ROLE IN PROPOSED PROJECT i Technical Advisor RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, J1st trsJning snd experience relev ant to area of project. Llat all relevant publications.) See Attached Curriculum Vitae Page 6B HONS 009132 BIOGRAPHICAL SKETCH Priority Area # Llt Ham Lee > Social Security I 521-52-6704 (Give the following Information for all profesalonal personnel beginning with the Principal Investigator. Use continuation pages and follov the saaa general fonsat for each person.) ' HAKE Joseph M. O'Connor, Ph.D TITLE Assist. Dir., Laboratory for Environmental Studies B1RTHDAIS (Mo..Gay, Tr.) 11/19/44 PLACE OP BIRTH (City, State, Country) Boston. Mass., USA Present Nationality (If non- Sex -s- cltl"n* indicate kind Jg. Mala of visa and expiration date) __Feaale EDUCATION (Begin with baccalaureate training and Include postdoctoral) TEAR SCIENTIFIC INSTITUTION AND LOCATION________DECREE CONFERRED________FIELD College of Holy Cross Worcester, Mass. B.S. 1966 Biology State U. of New York at Albany Ph.D. 1971 Ecology/Physiology HONORS */ Sigma Xi, Beta Beta Beta Biological Society Major research interest Environmental Pollution role in proposed project Technical Advisor . RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, ]lst training and experience relevant to area of project. List all relevant publications.) See Attached Curriculum Vitae Page 6 C HONS 009133 RESEARCH PLAN Priority Area I______ 7 i*3t Name Lee Social Security t 521-52-6704 BIBLIOGRAPHY: Provide e detailed bibliography that ie relevant to the reeearch project. List any reeearch performed by the principal investi gator (s) named in the application. Use continuation sheets __ If necessary._________________________________________________________________________________________ Ahmed, M. and D.D. Focht. (1973). "Degradation of polychlorinated biphenyls by two species of Achromobacter," Can. J. Microbiol. 19: 47. Alexander, M. (1965). "Biodegradation: Problems of molecular recalcitrance and microbial fallibility," Adv. Appl. Microbiol. 7: 35. Alexander, M. (1975). "Environmental and microbiological problems arising from recalcitrant molecules," Microbiol. Ecol. 17. Aulerich, R.J., R.K. Ringer, H.L. Seagran, and W.G. Youatt. (1971). "Effects of feeding coho salmon and other Great Lakes fish on mink reproduction," Can. J. Zool. 49: 611. Aulerich, R.J., R.H. Ringer, and S. Iwamoto. (1973). Reproduc tive failure and mortality in mink fed on Great Lakes Fish," J. Reprod. Fert. Suppl. 19: 365. Bruckner, J.V., K.L. Khanna, and B.H. Cornish. (1973). "Biological responses of the rat to polychlorinated biphenyls," Toxicol. Appl. Pharmol. 24: 434. Bryant, M.P. (1972). "Commentary on the Hungate technique for culture of anaerobic bacteria," Amer. J. Clin. Nutrition 25: 1324. Cahn, P., J. Foehrenbach, and W. Guggino. (1977). "PCB levels in certain organs of some feral fish from New York State," In: Physiological responses of marine biota to pollutants. F.J. Vernberg, A. Calabrese,~7. Thurberg, aneTw.B. Vernberg (eds.). Academic Press, Inc., New York, N.Y., p. 51. Choi, P.S.K., H. Nack, and J.E. riinn. (1974). "Distribution of polychlorinated biphenyls in an aerated biological oxidation water treatment system," Bull. Environ. Contamin. Toxicol. 11: 12. ------------ ----------- Corcoran, E.F., and C.C. Lee. (1970). "The distribution of chlorinated hydrocarbon pesticides in the Mississippi River delta and the adjacent Gulf of Mexico," Joint Southeast and Southwest ACS Meeting, New Orleans, La., p. 88. Duke, T.W., J.I. Lowe, and A.I. Wilson. (1970). "PCB (Aroclor 1254) in the water, sediment, and biota in Escambia Bay, Florida," Bull. Environ. Contamin. Toxicol. 5: 171. Pacts *7 MONS 009134 _2- tutlnuatioii Sl-^ct ^L-iat Mm: I,cp________ Social Security 0 521-52-6704 " Ecobichon, O.J. (1976). 'Enzymatic and other biochemical res ponses to selected PCBs," Hat'1 Conf. on Polychlorinated Biphenyls, Chicago, Illinois, p. 57. Fulk, R., D. Gruber, and R. Wullschleger. (1976). 'Laboratory study of the release of pesticide and PCB materials to the water column during dredging and disposal operations," Final Rept. of U.S. Army Engineer Contract No. DACW39-74eom. Furukawa, K. and F. Matsumura. (1976). 'Microbial metabolism of polychlorinated biphenyl components by Alkaliqenes sp.,* J. Agri. Food Chem. 24: 251. Gibson, D.T. (1968). 'Microbial degradation of aromatic com pounds,' Science 161: 1093. Guenzi, H.D. and W.E. Beard. (1967). 'Anaerobic biodegradation of DOT to DDD in soil," Science 156: 1116. Halvorson, H., M. Ishaque, J. Solomon, and O.W. Grussendorf. (1971). "A biodegradability test for insecticides," Can. J. Microbiol. 17: 585. Haque, R. and D. Schmedding. (1975). *A method of measuring the water solubility of hydrophobic chemicals,* Bull. Environ. Contam. and Toxicol. 14: 13. Hile, J.P. (1977). 'Polychlorinated biphenyls (PCBs): Unavoid able contaminants in food and food packaging materials: reduction of temporary tolerances," Federal Reqister 42: 17487. Hullar, T., R.M. Pleasant, S. Pagano, J. Spagnoli, and W. Stasiuk. (1976). "PCB data in Hudson River fish, sediments, water and wastewater,' Report prepared for PCB Task Force. Mew York State Health Advisory Council. Humphrey, H.E.B., H.A. Price, and M.L. Budd. (1976). 'Evaluation of changes of the level of polychlorinated biphenyls (PCBs) in human tissue," Final Rept. of FDA Contract No. 223-73-2209. Hungate, R.E. (1969). "A roll tube method for cultivation of strict anaerobes,* In: Methods in Microbiology 3B: 117. J.R. Norris and D.W. Ribbons (e3s.), Academic Press, Inc., New York, N.Y. Hutzinger, 0., S. Safe and V. Zitko. (1974). The Chemistry of PCBs, CRC Press, Cleveland, Ohio, p. 197. Page 8 HONS 009135 ( )onClnu.iCloii Sl-et ) Laoc Keau Leg Social Security tf 521-52-6704 Hydroacience Inc. (1978). 'Estimation of PCB reduction by remedial action on the Hudson River ecosystem,* Report to the New York Department of Environmental Conservation, pp. 107. Jensen, S. (1966). 'Report of a new chemical hazard,* New Scientist 32; 612. Kaiser, K. and P. Wong. (1974). 'Bacterial degradation of polychlorinated biphenyls. Identification of some metabolic products from Aroclor 1242,* Bull. Environ. Contamin. Toxicol. 11; 291. Kearney, p.C. and D.D. Kaufman. (1969). The Degradation.of Herbicides. Marcel Dekker, Inc., New York, N.Y. Kimbrough, R.D. (1976). 'Pathological findings associated with chronic experimental exposure to PCBs,* Nat'l Conf. on Polychlorinated Biphenyls, Chicago, Illinois, p. 14. Krull, I.S. (1977). 'Recent advances in PCB analysis,* In: Residue Reviews 66; 185. F.A. Gunther and J.D. Gunther (eds.), Springer-Verlag, New York, Inc. Kuratsune, M., T. Yoshimura, J. Matsuzaka, and A. Yamaguchi. (1972). 'Epidemiologic study on Yusho, a poisoning caused by ingestion of rice oil contaminated with a conuarcial brand of polychlorinated biphenyls,' Environ. Health Perspectives 1^ 119. Kuratsune, H., Y. Masuda, and J. Nagayama. (1976). 'Some of the recent findings concerning Yusho," Nat'l Conf. on Polychlorinated Biphenyls. Chicago, 111., p. 14. Lee, C.C. and R. Sansur. (Unpublished data on the growth of an aquatic fungus on Aroclor 1221, 1016, 1242 and 1254.) New York University Medical Center. Liu, D. (1976). 'PCB degradation,* Mar. Pollut. Bull. 7: 83. MacRae, I.C., K. Raghu and E.M. Bautista. (1969). 'Anaerobic degradation of the insecticide lindane by Clostridium sp.,* Nature 221: 859. Matthews, H.B. and M. Anderson. (1975). 'Effect of chlorination on the distribution and excretion of polychlorinated bi phenyls," Drug Metab. Dlspos. 3-. 371. Matthews, H.B. and M. Anderson. (1976). "PCB chlorination versus PCB distribution and excretion," Nat'l Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 50. Pag* 9 HONS 009136 1 < )ntlnuaClon SI "aC )Lat Social Security tf 521-52-6704 Hunson. T.O., H.D. Palmer, and S.M. Forns. (1976). 'Transport of chlorinated hydrocarbons in the Upper Chesapeake Bay,* Nat!1 Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 218: Nadeau, R.J. and R.A. Davis. (1976). 'Polychlorinated Biphenyls in the Hudson River (Hudson Falls-Fort Edward), New York State," Bull. Environ. Contamin. Toxicol. 16; 436. Nebeker, A.V. and F.A. Fugles. (1974). "Effect of polychlori nated biphenyls on survival and reproduction of Daphnia. Gammarus, Tanvtarsus." Trans. Amer. Fish. Soc. 7i2. New York State Dept, of Environmental Conservation. (197$). "In the matter of alleged violations of Sections 17-0501, 17-0511 and 11-0503 of the Environmental Conservation Law of the State of New York by General Electric Company,' Interim Opinion and Order File No. 2833. Nimmo, D.R., D.J. Hansen, J.A. Couch, N.R. Cooley, P.R. Parrish, and J.X. Lowe.. (1975). "Toxicology of Aroclor 1254 and its physiological activity in several estuarine organisms," Arch. Environ. Contamin. Toxicol. .3s 22. Nisbet, I.C.T. and A.F. Sarofim. (1972). "Rates and routes of transport of PCBs in the environment," Environ. Health Perspectives 1_: 21. Norback, D.H. and J.R. Allen. (1972). "Chlorinated aromatic hydro carbon induced modifications of the hepatic endoplasmic reticulum: concentration membrane arrays," Environ. Health Perspectives 1: 137. O'Connor, J.M., L. Peters, and R. Califano. (Unpublished data on the uptake on Aroclor 1254 from Hudson River water and Hudson River forage by Morone saxatilia.) New York University Medical Center. Raghu, K. and I.C. MacRae. (1966). "Biodegradation of the gamma isomer of benzene hexachlori.de in submerged soils," Science 154: 263. Risebrough, R.W. (1969). "Chlorinated hydrocarbons in marine ecosystems," In: Chemical Fallout. M.H. Miller and G.G. Berg (eds.), C.C. Thomas, Springfield, 111., p. 5. Risebrough, R.W. and B.W. de Lapps. (1972). "Accumulation of polychlorinated biphenyls in ecosystems," Environ. Health Perspectives 1: 39. Roach, J.A.G. and X,H. Pomerantz. (1974). "The finding of chlorinated dibenzofurans in Aroclor PCBs of recent Pag* 10 M0NS 009137 Continuation Sl->'et Laot Mese Lae ) Social Security J 521-52-67na As with the chlorinated pesticides the PCBs are relatively insoluble in water and are highly persistent in the environ ment (Risebrough and de Lappe, 1972). These compounds are not easily metabolized by enzyme systems presently found in nature (Choi et al., 1974; Alexander, 1975) and tend to accumulate in fatty tissues and oils of animals (Risebrough and de Lappe, 1972). Because of their low water solubility, high specific gravity and high adsorbability, PCBs accumulate in anaerobic or reducing sediments of rivers, estuaries and the coastal zone. (Duke et al., 1970; Fulk et al., 1976; Munson et al., 1976). Although PCBs were first detected as environmental con taminants in 1966 (Jensen, 1966), their potential impact as a health hazard was not realized until 1969, after the discovery and description in Japan of Yusho, a human disease caused by consumption of PCB-contaminated rice oil (Kuratsune et al., 1972). Since then, PCBs have been implicated in environmental and occupational diseases ranging from chloracne and liver damage to reproductive failure. They may also act as carcinogens or co-carcinogens (Norback and Allen, 1972; Kuratsune et al., 1972, 1976; Kimbrough, 1976; U.S. Department of Health, Education and Welfare, 1978). Laboratory studies with industrial preparations of PCBs (e.g., Aroclor 1242, 1254 and others), and with pure isomers of chlorinated biphenyls have shown PCBs to be highly toxic rags 13 HONS 009138 1 'I I rntinuJCion SI |L.iot Mgau Log__ Social Security & 521-52-67H4 to many aquatic and terrestrial organisms (Bruckner et al., 1973; Grant and Phillips, 1974; Stalling and Mayer, 1972; Nebeker and Pugles, 1974; Nimmo et al., 197S; Vos, 1972; Walker, 1976). Those organisms which are not immediately affected by PCBs may accumulate them to high concentrations, thereby increasing the exposure of other organisms through food chain transport (Risebrough, 1969; Cahn et al., 1977; O'Connor et al., unpublished data). ' It has been shown that the toxicity of a given PCB depends upon the degree of chlorination and its isomeric form. Chlorinated biphenyls that have a low percentage of chlorination tend to have a high acute toxicity compared to chlorinated biphenyls with a greater percentage of chlorina tion (Matthews and Anderson, 1975). The results from compara tive studies of chronic exposures of animals to specific isomers are confusing and inconclusive, but the exposure of test animals to subacute levels of individual PCB preparations (e.g., Aroclor 1242, 1248, 1254, and 1260) resulted in varying degrees of liver dysfunction, which was inversely related to the chlorine content of the Aroclor (u.S. HEW, 1978). Lower homologs are also usually more soluble in water than the higher ones (Haque and Schmedding, 1975), and are therefore more available to, and more rapidly absorbed and metabolized by, aquatic organisms. PCBs which can be metabolized and excreted may introduce toxic metabolites to the environment, while the forms of PCB that are not readily HONS 009139 Page 14 Continuation SI "at (i Lact Maai Lwp Social Security II 521-52-6701 manufacture," presented at 85th Annual Meeting Assoc. Official Analytical chemists. Paper No. 53, Washington, D.C. October 14-17. Sayler, G.S., M. Shon and R.R. Colwell. (1977). "Growth of an estuarine Pseudomonas sp. on polychlorinated biphenyl," J. Microbial Ecol. 3: 241. Sayler, G.S., R. Thomas and R.R. Colwell. (1978). "Polychlori nated biphenyl (FCB) degrading bacteria and PCS in estuarine and marine environments," Estuarine and Coastal Marine Science 553. Stalling, D. and J. Huckins. (1973). "Reverse phase thin layer chromatography of some Aroclors, Halowaxes, and pesticides," J.A.O.A.C. 56: 367. ' Stalling, D. and F.L. Mayer. (1972). "Toxicities of PCBs to fish and environmental residues," Environ. Health Per spectives ]L: 159. United States Department of Health, Education and Welfare. (1977). "NIOSH criteria for a recommended standard...occupational exposure to polychlorinated biphenyls (PCBs)," PHEW (NIOSH) Publ. No. 77-225. Vos, J.G. (1972). "Toxicology of PCBs for mammals and birds," Environ. Health Perspectives 1: 105. Vos, J.G., J.H. Koeman, H.L. Van der Mass, M.L. Ten Noever de Brauw, and R.H. de Vos. (1970). "Identification and toxi cological evaluation of chlorinated dibenzofuran and chlori nated naphthalene in two commercial polychlorinated bi phenyls," Food Cosmet. Toxicol. 8: 625. . Walker, C.R. (1976). "The occurrence of PCBs in the national fish and wildlife monitoring program," Hat'l Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 161. Wiese, C.S. and D.A. Griffin. (1978). "The solubility of Aroclor 1254 in seawater," Bull. Environ. Contamin. and Toxicol. 19: 403. Wong, P. and K. Kaiser. (1975). "Bacterial degradation of polychlorinated biphenyls II. Rate studies," Bull. Environ. Contamin. and Toxicol. 13: 249. Pag* ii HONS 009140 L> pclnuatlon Sl :ct f- at: ilszae Lee Social Security J 521-52-6704 A. INTRODUCTION 1. Objective; This research will investigate the rates at which aerobic or anaerobic microorganisms degrade PCBs in rivers, estuaries, or landfills. The investigation is designed also to test the hypothesis that the toxicity of PCB residues is gradually lessened in situ by the fact that the lower chlorinated isomers are more rapidly degraded than the higher chlorinated isomers which are Jcnown to be less active biologically and less toxic as well. 2. Background: Polychlorinated biphanyls (PCBs) are among the most persistent contaminants man has introduced to the environment. These compounds are a versatile group of industrial chemicals that have been widely used for more than 40 years. Two-hundred-nine possible isomers of chlori nated biphenyls exist, and each industrial PCB preparation may contain ten or more isomers, as well as impurities, such as polychlorinated bibenzofurans (PCDFs), polychlorinated dibenzodioxans (PCDDs) and other unidentified products (Vos et al., 1970; Roach and Pomerantz, 1974; Hutzinger et al., 1974) . These products have been released into the nation's fresh and coastal waters by deliberately discarding PCBs or PCB-laden products into landfills and dumps, or as effluents into rivers (Nisbet and Sarofim, 1972). A small amount is lost into the air from evaporation from plasticized products or from incompleteburning of substances containing PCBs. Page 12 MONS 009141 Continuation Si vet Lace Social Security 0 521-52-6704 metabolized have an extremely long biological half-life (Matthews and Anderson, 1976). It has been shown also that isomers having two adjacent unsubstituted carbons in the biphenyl ring are more readily metabolized than ones without the two adjacent unsubstituted carbons (Ecobichon, 1976). The net effect of the above is that different PCBs have different toxicities and different rates of bioaccumulation and persistence. The evidence in general establishes that ?CBs with five or more chlorine atoms are less biodegradable and are accumulated to higher levels in organisms and persist longer in the environment. PCBs from aquatic environ mental samples, such as fish, show on analysis a pattern of peaks approaching that of PCB mixtures with more than 501 chlorination, while the major input of PCBs into the environ ment was PCB mixtures containing 421 or less chlorine (Matthews and Anderson, 1976). Highly chlorinated Aroclors, those containing 501 or more chlorine, comprised approximately 301 of sales prior to 1971; this figure was expected to decrease to 221 after 1971 (Nisbet and Sarofim, 1972). This lends support to the laboratory results described above, that the rate of metabolic attack on PCBs decreases with increasing chlorination. It is therefore possible that PCB residues found in natural waters and sediments and in aquatic organisms which concentrate PCBs via long food chains may not have originated entirely from one specific industrial preparation such as Aroclor 1242, 1016 or 1254. Instead, they may *9 is HONS 009142 2 1 bntlnu.ition ut Al iM ) I-'Uit (.'ilUt! ___kpo Social Security J 521-52-G7Q.I represent only the higher homologs of several PCB preparations, the lower homologs of which were metabolized or otherwise degraded along the food chain. The consumption of fish contaminated with PCBs from polluted lakes and streams appears to be the major source of PCB exposure for man and animals. However, there is no record of clinical damage to humans from such consumption (Humphrey et al., 1976; Hile, 1977), even though the amount of PCBs ingested was high compared to people who ate no fish. This being the case, it becomes unclear whether this lack of adverse health effects from the consumption of fish contaminated with PCBs from the environment is due to the low level of exposure or because the PCBs in the fish are primarily isomers that have a low-grade toxicity; if so, the more active, more toxic isomers may have been degraded in the environment to less toxic forms. The persistence of some chlorinated pesticides and hydrocarbons and the apparent stability of some of these compounds in the environment fostered the idea of molecular recalcitrance (Alexander, 1965, 1975). As a result, the biodegradation of PCBs has received little attention and few data exist on the rate at which PCBs are being degraded in nature. However, it has been concluded by many investigators from laboratory studies that PCB isomers containing four or fewer chlorine atoms are degraded rapidly in the environment by microbial activity. Sayler et al. (1978) showed that PCB HONS 009143 lft I ^Continuation I.! et L-u-.t 1 r^,,, Social Security if 3M-52-r,7(l.l resistant, or degrading, bacteria were ubiquitously distributed and indicated the potential for PCB degradation by natural microbial flora in estuarine and marine environments. Available reports on PCB metabolism by microorganisms have dealt exclusively with aerobic bacterial systems (Ahmed and Focht, 1973, Kaiser and Wong, 1974; Wong and Kaiser, 1975; Furukawa and Matsumura, 1976; Liu, 1976, Sayler et al., 1977, 1978). Published works which indicate that chlorinated pesticides and other aromatic compounds are degraded under anaerobic conditions (Raghu and MacRae, 1966; Suenzi and Beard, 1967; MacRae et al., 1969; Focht and Ahmed, 1970) suggest that the lower PCB isomers in sediments may be degraded primarily by anaerobic bacteria. Wbrk begun in our laboratory with an aquatic fungus (Lee and Sansur, unpublished data), isolated from Hudson River water and grown in Aroclqr 1254, together with the works of Sayler et al. (1978) and Xearney and Kaufman (1969) indicate that fungi may also be actively involved in the PCB degradation process. 3. Rationale: PCB contamination in the Hudson River is probably the most extensive in the nation. There are an estimated 450,000 to 600,000 pounds of PCBs in the bottom sediments of the upper Hudson River between Fort Edward and Troy, New York. Although no reliable estimates of PCB content in the bottom deposits of the lower Hudson from Troy to New York City are available, PCB content is expected to MONS 009144 / i i : M . i j Aco.i Ccntinu.Uion SI 1 i!t thou f,op Social Security / 52l-!32-f>70-l be substantial. The major portion is adsorbed to particles deposited in anaerobic or reducing sediments near the point of PCB discharge. Even without further discharge, these PCBs will remain for some years and will continue to contami nate river biota unless the PCBs are degraded or are resioved by water currents, dredging or by bioaccumulation in aquatic organisms. Thus far, degradation studies have centered on aerobic bacterial activity, whose results have little appli cation to the problem at hand, because most of the PCBs are sequestered in anaerobic or reducing sediments. Until now, the role of fungi in PCB degradation has not been considered at all. This study is proposed to complement the existing work on PCB degradation to achieve a more complete understand ing about the fate of these PCB deposits. As man is the ultimate consumer after eating contaminated fish, and in light of the uncertainties described above regarding the differential toxiclties of different PCB preparations, it is our intent, also, to examine the toxicity of the PCB mixture remaining after microbial action on key Hudson River organisms relative to the documented toxicities of non-degraded PCB mixtures. This information will make it possible to predict the future public health and ecological significance of the PCB deposits. MONS 009145 ''Continuation nt ) LniiC ___T'l"'l"' Social Security <7 521-52-07(1.1 B. SPECIFIC AIMS 1. To isolate and cultivate from Hudson River sediments anaerobic bacteria and fungi which are able to grow in the presence of PCBs (Aroclor 1242, 1016 and 1254) and to investigate their ability to degrade them. Initially, this will be done with mixed cultures; studies will be done with pure cultures as they become available. Aerobic bacteria will not be included in this work, as these organisms are being studied by other investigators. 2. To determine the rate of PCB degradation by anaerobic bacteria and fungi thus isolated and to characterize the resulting metabolites and residues. 3. To examine the relative toxicity of the PCB mixture remaining after microbial action against non-degraded PCB mixtures on some key Hudson River organisms such as Gammarus, Meomysis and striped bass larvae. C. METHODS 1. Procedures for Specific Aim 1; Sediments for the isolation of anaerobic bacteria and fungi will be collected from at least two sites in the Hudson River known to be contaminated with PCBs, one in fresh water and the other in the saline portion of the river, with a sediment grab lowered from a boat. Immediately upon collection, the sediment will be transferred to duplicate sets of glass bottles which will be almost filled with filtered river water and sealed with serum caps. One set will be gassed with compressed nitrogen HONS 009146 loutinuatiou 1 - ul Social Security it 521-52-f, 711.1 to maintain reducing conditions necessary for anaerobes; the other will be maintained aerobic for fungal isolates. Both sets of samples will be returned to the laboratory for subsequent isolation of PCB degraders by enrichment culture. Enrichment cultures for anaerobic bacteria will be set up by adding 1-2 g of sediments to a 250 mi flask containing 100 mi of an organic medium: glucose, 0.5 g; peptone, 0.5 g; KHjPOj, 0.5 g; distilled water or water of appropriate salinity, 1 i (pH 7.0). Techniques for anaerobic cultures as described by Bryant (1972) will be strictly followed. Cultures will be incubated for 3 days at 26C; intermittently, culture flasks will be shaken by hand. Isolation will be carried out in a second flask which contains 100 mi of a mineral salt solution: (NH4)2S04, 1 g; KHjPO^, 0.2 g; KjHOP^, 1.6 g; MgS04-7H20, 0.2 g; NaCl, 0.1 g; PeS04*7H20, 0.01 g; CaCl2'2H20, 0.02 g (pH 7.5) containing 0.05% of the appropriate PCB. Following standard anaerobic techniques 1 ml of the enrichment culture will be transferred to this second medium and incubated as before until substantial growth is observed. Such cultures will be maintained in duplicate or triplicate. One series will be incubated in the light and one in the dark to enable the growth of phototrophic bacteria as well as strict chemotrophs and heterotrophs. Enrichment cultures for fungi will be started in Sabouraud dextrose agar (Difco) and incubated under aerobic conditions. After initial growth, 1 mi of the enrichment culture will be HONS 009167 20 j > ' j Socl.il S'.xur!tv 1 transferred to the PCB-mineral salts medium (described above) but amended with 1 mi of a trace elements solution per liter. The trace elements solution contains 0.1 g HjBOj, 0.05 g each of CuSC^-5 HjO, MnS04>H20, ZnS04*7 HjO, Na2Mo04, and CoClj-6 HjO per liter. After incubation for predetermined time intervals (4 days to 1 month), aliquots of the incubated samples will be transferred to separatory funnels and extracted with hexane. Gas chromatograms will be run with 1-5 pi aliquots of the hexane extracts. Initially, only the disappearance of certain PCB isomers from the mixed culture systems will be measured. If the PCBs disappear or decrease in quantity, degradation products will be looked for, perhaps using thinlayer chromatographic techniques and with 14 C-labelled PCBs. Particular emphasis will be placed upon the water-soluble products. These will be isolated, after hexane extraction, by column chromatography on ion-exchange columns and resolved by thin-layer chromatography. Mixed cultures will be purified by plating onto mineral salts agar plates containing 0.051 PCBs. 2. Procedures for Specific Aim 2; Once the respective microbial systems capable of growth on PCBs are obtained, the kinetics of PCB degradation will be followed by analyzing the PCBs in the growth medium at different time intervals. The cells will be harvested by centrifugation and washed twice with 0.5 M phosphate buffer (pH 7.0). The washed cells will be MOMS 009148 21 ^ ^ Social Security H 521-52-1,71).! injected into culture tubes containing the mineral salts medium and PCB. The procedures employed will be similar to those described by Halvorson et al. (1971) who used resting uspensions of bacteria harvested from a sewage lagoon to examine the degradation of mixtures of pesticides. Comparisons will be made of gas chromatograms of PCBs before and after incubation with test organisms over the predetermined time interval. Metabolites and residual PCBs will be characterized by the use of column and thin-layer chromatographic techniques (Stalling and Huckins, 1973; Furukawa and Matsumura, 1976). 3. Procedures for Specific Aim 3; PCB residues and residual PCB isomers from microbial activity on the original Aroclor will be recovered from thin-layer chromatograms by scraping the respective spots off the plates and dissolving in acetone. Their toxic effects, if any, will be assessed on the physiology and population dynamics of some key Hudson River organisms (Gammarus, Neomysis and striped bass and other fish larvae) relative to the non-altered Aroclor. These organisms represent important fish food items and at times comprise the majority of zooplanktonic or benthic species in various portions of the Hudson River, and are routinely cultured in our laboratory. Cultures of each species will be established and exposed to varying levels of PCBs, PCB residues and metabolites in static systems. Biological effects of PCB exposure will be estimated from several critical parameters: MOMS 009149 22 ( fconCinunUou i! C , ) j'asi!____ ' Social Socuricy (/ 521-32-fi7i).l a. rate of individual growth b. rate of respiration c. rate of production of young d. limits of tolerance to selected stresses: e.g., chlorine exposure, osmotic stress, thermal stress All of the above parameters will be estimated for cultures receiving PCB stress, as well as those cultured in the absence of PCBs. The data gathered here will be analyzed following basic toxicological approaches to threshold sensi tivity of organisms to toxicants and will be subjected to standard parametric and nonparametric statistical tests. 4. The research tasks will be performed in the following sequence, assuming a beginning data of January 1, 1979: January to March: Sample collection and culture isolation March to July: Degradation experimentation and analysis July to October: Toxicity experimentation November and December: Report preparation D. SIGNIFICANCE In light of our present knowledge about the toxicological properties of certain PCB isomers on living organisms and the knowledge that there might exist different rates of degradation for each of the 209 PCB isomers, it is expected that the health risks from PCB accumulations may change with time. This study should provide data for the assessment of long term health risks from PCBs and their residues in the aquatic environment. MOWS 009150 23 I/ JftntJmi.'ition St it ^ I-IUC Social Security if 521-52-fi 70 ! E. FACILITIES AVAILABLE The proposed research will be carried out using the facilities of the analytical chemistry and aquatic ecology sections of the Laboratory for Environmental Studies. The proposed research will be carried out in the culture and analytical facilities at the A.J. Lanza Laboratory, Tuxedo, New York, and at the Hudson River field station maintained by the Medical Center at Buchanan, N.Y. NYU boats equipped for collection of samples and certain field analytical procedures are berthed at Stony Point, N.Y. and operate throughout the open-water months. Dock facilities are available at the Buchanan wet-lab, but only on a temporary basis. Equipment available for this project includes apparatus for: Extraction/concentration of organics Freeze-dry techniques Scintillation spectrometry (low level - beta) Gel electrophoresis Thin-layer chromatography Gas-liquid chromatography High-pressure liquid chromatography Respiration studies Water quality analysis Autoradiography HONS 009151 7d i Continuation S) et { Lact Ncau Lpp__ Social Security tf 521-52-6704 Culture facilities and equipment for aerobic and anaerobic microorganisms Culture of aquatic organisms Maintenance of aquatic organisms Computer data analysis P. PRINCIPAL INVESTIGATOR ASSURANCE "The undersigned agrees to accept responsibility for the scientific and technical conduct of the research project and for provision of required progress reports if a grant is awarded as a result of this application." Date Principal Investigator Page 25 HONS 009152 U  EH TOUC STATE HEALTH RESEARCT COUNCIL ' ' PROTECTION OF BOHAN SUBJECTS Assuraaca/Cartificacloa/Daclaracioa (Furaoaae to Federal and State Law) #7 Lee 521-52-670 0 Great . ' Q Fellow I H?.C - i SXAXUOaT OF FOLICT: Safeguarding the rights and welfare of subjects at risk in activities supports! under grants and contract from NTSHRC is primarily the responsibility of the institu eloa which receives or is accountable to HTSHRC for the funds awarded for che support at the activity. In order to provide for the adequate discharge of this institutional responsibility It is the policy of NTSHRC that no activity involving human subjects to be supported by TTSaat grants or contracts shall be undertaken unless the Institutional Bevies Board has reviewed ___` approved such activity, and che institution has submitted to NTSHRC a certification of such review and approval, in accordance with che requirements of Chapter 450, NTS Laws of 1975. 1. TITLE OF PROPOSAL OR ACTIVTTT: The Environmental Health Implications of Aerobic . and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) '_______ <" lli^enn .Bttiiary----------------------------------------------- --------------------------------------------- --- -------- 2. Principal Investigator/Activity Director/Tellow Chun C. Lde, Ph.D.' ' 3. Declaration that a-- Subjects Either Would or Would Hoc Be Involved A. No Individuals who might be considered human subjects, including those from whoa O organs, tissues, fluids, or ocher materials would be derived, or who could be identified by personal data, would be involved la the proposed activity. (If 00 h--" subjects would be Involved, check this bor and proceed to Item 5. Proposals determined by che Agnacy to involve human subjects will be returned.) 1. mtmmr, subjects would be Involved in the proposed activity as either: I I None of tha following, or including: 0 Minors, 0Fetuses, 0 Abortuses, 0 Pregnant Women, f~~i Prisoners, 0 Mentally Retarded, 0 Mentally Disabled. Under Section 7, Cooperating Institutions, on reverse of this fora, give name of institution sad "* and address of offlclal(s) authorizing access to any subjects In facilities not under direct control of the applicant or offering institution. 1. Declaration of Assurance Status/Certification of Review A. This institution has not previously filed an assurance and asauraaca implementing procedures for che protection of human subjects with the Council that applies to rh-te application or activity. Assurance is hereby given chat this Institution will comply with requirements of Chapter 450, NTS Laws of 1975, that it has established an Institutional review board for the protection of human subjects and, shea requested, will submit documentation and certification of such reviews and procedures as may be required for implementation of this assurance for the proposed project or activity. |_JR. tm institution has an approved general assurance or an active special assurance for tM m ongoing activity, on file with tha Council. Tha signers certify that all activities in this application proposing to involve human subjects have been reviewed ' and approved by this institution's Institutional review board in a convened meeting on the dace of THE INSTITUTIONAL REVIEW BOARD HAS DETERMINED .AND THE INSTITUTIONAL OFFICIAL SICTOIC BELOW CONCURS THAT: Either 0 human subjects will, not be ag risk,or 0 human subjects will be at ri. S. Name and Address of Inaclcutiaa A. Tlcls of Institution Official ________________________________________________________;________________________________ phone: Signature of Institutional Official Data MONS 009153 ( rrv*^'' fr n i . NOt AP*1\C<mNT?<kHi&b 521-52`6704 ) Title of Invention Grant or Coatrace Under Which Developed Identifier Date of Report Inventor's Nasw(s) Official Title(a) Business Addreee Facility Where Invention Made Name of Unit or Building Address DESCRIBE INVENTION III DETAIL FOLLOWING OUTLINE BELOW. ATTACH MAPS, SKETCHES, PRINTS, PHOTOS TO DESCRIPTION. A. General Purpose - State In general taras the purpose end object of the Invention B. Background - Describe prior art (Identify by patent number or Journal citation) C. Description and operation 1. Describe completely the construction of invention using reference characters to identify components In attached illustrations. 2. Describe one (1) complete operational cycle. 3. If invention relates to a synthesis or identification of a new composition of matter, describe the product In structural form, if possible, and the process of making it. Include all available information regarding physical characterlstlca end all test data evidencing utility.________________________________________ ____________ DESCRIBE COMMERCIAL POSSIBILITIES (INCLUDE QUANTITY AND PRICE RANGE IF AVAILABLE) STATE REASONS FOR APPLYING FOR PATENT STATE PLANS TO PUBLISH NAME OF PUBLICATION, ATTACH OUTLINE OF ARTICLE. IF ALREADY PUBLISHED, INCLUDE JOURNAL CITATION OR ATTACH REPRINT. DESCRIBE AND GIVE DATE(S) OF ANY SALE OR PUBLIC USE OF INVENTION (SPECIFY IF USE WAS OPERATIONAL OR FOR TESTING PURPOSES) CONTRIBUTIONS TO THE INVENTION BY: GRANTEE NYSHR COUNCIL OTHER (Identify) PERSONNEL (No. of Personnel) _ MATERIALS (List Type and Value) _ FINANCIAL CONTRIBUTION _ EXECUTION OF DISCLOSURE I, , state the above Information with regard to the Invention of name of lnventer title of Invention the invention. is a true and correct disclosure of data pertlnenc (O Technical Witness (2) Signed Dace MONS 009154 u 1 7. Cooperating Institutions - Additional Reporting Requirement Dm Following Report Format for Each Inatlcueion Other Than Grantee or Contracting Xnatitution with Responsibility for Human Subjacts Participating In this Activity: (Attach additional report ahaeca aa necessary) ZHSTITOTIONAI. ASTH0RIZAZ10H FOR ACCESS TO SUBJECTS Subjacta: Status (Warda, Residents, Employaaa, Patients, Etc.)___________________ Humber Ace Baace Title game and Address of Cooperating Institution . ' ...... Pfeono Official Signature c HONS 009155 . ' r HZff TOXX stats BXALTH awmerg COCSCTX AI2HI *HD COTTXICHT AfiMBOWT Dm modarslgned applicant, la conaiteration of a grant/contract awarded by tha Saw Tork Stata Baalth Research Council, doaa hereby agree aa followa: (a) Ts keep tha Connell informed of any potentially patantabla device or tachinqu* conceived or developed by the undersigned, la whole or la part, Individually or with others. . (b) That a patantabla da-rlca or tachlnque conealvad or davmlopad la eha eouraa or working on prograaa or proJacts funded by tha Council ahall ba tha praparty of tha Council. . (c) That royaltlaa, or any othar remuneration received by tha - applicant will ba promptly remitted to tho Council and rediatrlbuted to the applicant and tha inventor (a) paranant to Council policy. c (d) Upon Instruction of eha Council. to apply for a patent on any ouch patantabla device or technique and aaalgn to tha Council or . its nominee all ay rlghe, title and lntaraat in such patantabla device and eracute, acknowledge and deliver to tha Council any . instruments necessary or raquizad by eha Council to affactuata ~ such application, assignment of this agreement. . (a) To conform with and adhere to tha patent and copyright policy of eha Council, a copy of which has been received by eha undaralgned. as tha asms may from time to tlaa ba amended. ' (f) To give appropriate credit to tha Council in any publication or other fora of disclosure or at tha request of eha Council to provide an appropriate disclaimer to eha affeet that eha publication or disclosure does not represent eha official position of tha Cornell. (g) A description of any invention, whether aada or conealvad by employees or agents of tha undersigned prior to funding by eha Council, whacher patented or not, la appended hereto end such inventions shall ba excluded from this agreement. I (va) represent ehae the absence of any invention in eha attached schedule shall indleata that I (wa) own no such invention at tha time of signing this agreement. HONS 009156 c (Signature) /O -Jj'7-??' (Data) i H&C-23 APPENDIX: NYU Organism Culture The New York University Medical Center presently maintains in culture several aquatic species, including protozoa, algae, zooplankton, benthic biota, fish, and fish larvae. The species of algae in culture include: Chlorella. Nannochloris, Chlamydomonas, Melosira, Synedra. Anacvstis. and others. Protozoan species include Strombidium, Euplotes. Oronychla. and Paramecium. Many different types of microzooplankton have been maintained at the Medical Center. Included among them are: Mesocyclops cdax, Eurytemora affinis. Acartia clausi, Daphnia magna, and Cyclops blcuspldatus. Ostracods are presently being cultured in the laboratory for a study of breeding behavior. Benthic biota maintained at the - Laboratory include Carcinus maenas, Rlthropanopeus harrisi. and various species of gastropods. The crabs have been used in a series of PCB uptake studies, as well as in studies of respiration as affected by hydrogen sulfide and low oxygen tension. Several macrozooplankters are maintained at the Laboratory at all times. These include Gammarus daiberi, B. tlgrinus, G. fasdatus. Seomvsis americana, and the larvae of Chaoborus punctipennls. The three species of Gammarus have been maintained for more than four years in aquarium culture, and in incubators at a variety of different temperatures. Morone saxatills, Cyprinus carplo. Fundulus heteroclitus. and Microgadus tomcod are examples of fishes and fish larvae which have been raised and maintained in our laboratory facility. Striped bass and tomcod have been spawned and raised in NYU facilities each year since 1975. These laboratory-raised organisms have been used successfully in studies of temperature tolerance, chlorine toxicity, behaviour, and growth. The following references provide examples of studies conducted by the HONS 009157 ? NYU staff using organisms produced at, or maintained in, NYU culture facilities: 1. THESES Eichorn, K.L., 1977. The response of striped bass (Morone saxatilis) to heated and chlorinated power plant effluent. M.s7 Thesis, Biolgoy Dept. New York University. Ginn, T.C., 1977. An ecological investigation of Hudson River macrozooplankton in the vicinity of a nuclear power plant. . Ph. D. Diss., Biology Dept., New York University. Jinks, S.M., 1975. An investigation of the factors influencing radiocesium concentrations of fish inhabiting .natural aquatic systems. Ph. D. Diss. Biology Dept., New York University. i Paschoa, A.S., 1975. The natural radiation dose from radium to Gammarus in the Hudson River. Ph.D. Diss. Sch. Eng. and Science, New York University, Poje, G.V., 1977. Growth and reproduction of Gammarus tigrinus Sexton, 1939. M.S,' Thesis., Biology Dept. New York University. ' .. .. 2. PUBLICATIONS Beck, A.P.., G. Poje, and W. Haller., 1975. A laboratory study on the effects of exposure of some entrainable Hudson River biota to hydrostatic pressure regimes calculated for the proposed Cornwall Pumped Storage Plant, pp. 167-204 In: S. Saila, ed., Fisheries and Energy Production. D.C. Heath, Lexington, Hass. Becker, A.J., 1977. The respiratory responses of the green crab Carcinus maenas to reduced partial pressures of oxygen. Limnol. Oceanogr. in press. Ginn, T.C. and J.M. O'Connor, 1978 (in press). The responses of Gammarus ticrinus to chlorinated power plant effluents. Estuarine Coastal Mar. Sci. Ginn, T.C., W. Haller, and G.J. Lauei., 1974. The effects of power plant condenser cooling water entrainment on the amphipod Gammarus spp. Hater Res. 8:937-945. Ginn, T.C. W. Haller, and G.J. Lauer., 1976. Survival and repro duction of Gammarus spp. (Amphipoda) following short-term exposure to elevated temperatures. Ches. Sci. 17:8-16 HONS 009158 ) O'Connor, J.M. and S. Schaffar. 1977. Survival of striped bass ichthyoplankton in nets. Ches. Sci., 18:312-315. Poje, G.V., T.C. Ginn and J. H. O'Connor. 1978. Responses of ichthyoplankton to stresses simulating passage through a power plant condenser tube. In: Thorp, J. and J.W. Gibbons (eds.). Energy and environmental stress in aquatic systems. Symposium of the Savannah River Ecology Laboratory, 1977. Rachlin, J., A.P. Beck and J.M. O'Connor. 1978. Karyotypic analysis of Hudson River striped bass (Morone saxatllis). Copeia, 1978: in press. Zubarik, L.S. and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. In: Thorp, J/ and J.W. Gibbons (eds.). Energy and environmental stress in aquatic systaias Symposium of the Savannah River Ecology Laboratory, 1977. 3. PUBLICATIONS IN REVIEW Ginn, T.C. and J.M. O'Connor. The distribution of Gammarua daiberi in the Hudson River estuary with a note on a range extension for the species. Crustaceans... O'Connor, J.M., K.L. Eichorn and T.C. Ginn. Behavioral response of larval and juvenile striped bass to heated and chlorinated power plant effluents. Estuaries... O'Connor, J.M., G. Poje and S. Rigdon. Respiration of larval forms of the striped bass (Morone saxatllis) following short-term ex posure to elevated temperatures. Exp. Mar. Biol. Ecol... HONS 009159 4. PRESENTATIONS ) ) Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of the striped bass Morone saxatills and the amphipod Gammarus to chlorinated power plant effluent. Northeast Fish and Nildlife Conference. April 26-29, Hersey, Pennsylvania. O'Connor, J.M., C.C. Coutant and J.P. Lawler. 1977. Environmental impact assessment as ecological research. Symposium on Energy and Environmental Stress in Aquatic Systems. Augusta, Georgia, November, 1977. Poje, G.V., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrainment effects. Northeast Fish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. ......................................................................................................... 1977. An operational system for simulating the physical and chemical environment' of the con denser tubes of a power plant. Symposium on Energy and Wnvironmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tigrlnus: an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society, May, 1976. Zubarik, L.S. and J.M. O'Connor. 1977. A radioisotopic study of mercury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November 1977. HONS 009160 (. > CURRICULUM VITAE January 1978 Chun Chi Lee (Formerly Known as King Mon Tsue) Date and Place of Birth: Marital Status: Residence: Professional Address: Title: Education: Positions Held: Course Offering: (Department of Environmental Medicine and Department of Biology) ` August 31, 1939 - Hong Kong Married, Two children R.D. (2, Felter Hill Rd. Monroe, New York 109S0 New York University Medical Center Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext. 5276 Research Scientist and Adjunct Assistant Professor Laboratory for Environmental Studies 1962 - B.S. in Zoology Colorado State University 1969 - Doctor of Philosophy in Marine Science University of Miami (Florida) New York University Medical Center Department of Environmental Medicine 1975 - Present Aquatic and Estuarine Microbiology University of Miami (Florida) Rosenstiel School of Marine and Atmospheric Science, 1969-1975 Research Scientist, Functional Biology 1969 - 1973 Research Assistant Professor Biology and Living Resources 1973 - 1975 . HONS 009161 1 I) Positions Held: Professional Societies: Research Interests: Awards and Grants: ) University of Miami (Florida) Institute of Marine Science 1962 - 1969 NDEA Fellow, Marine Biology 1962 - 1965 Graduate Assistant 1965 - 1967 Senior Field Biologist McMurdo Sound, Antarctica 1967 - 1968 Senior Field Biologist First International Weddell Sea Oceanographic Expedition . 1968 - 1969 Colorado State University Department of Botany and Plant Pathology 1958 - 1962 Research Assistant, 1958 - 1962 American Association for the Advancement of Science American Society of Limnology and Oceanography Microbiology Algal and Protozoan Physiology and Ecology Biochemistry of Organic Matter in Aquatic Sediments Achievement Award in Biological Science Colorado State University, 1962 National Defense and Educational Act Predoctoral Fellowship, 1962 - 1965 National Science Foundation Grant University of Miami, $44,000 1973 - 1975 National Science Foundation Grant New York University Medical Center, $3,900 1976 MQNS 009162 PUBLICATIONS C. C. LEE 1. Meyers, S. P., W. A. Feder and King Tsue. Nutritional relation ships among certain filamentous fungi and a marine nematode. Science, 141, 520-522 (1963). 2. Meyers, S. P., W. A. Feder and King Tsue. Studies of relation ships among nematodes and filamentous fungi in the marine environment. Dev. Indus. Microbiol., 5^, 354-364 (1964). 3. Altman, J. and King Tsue. Changes in plant growth with chemicals used as soil fumigants. Plant Disease Reporter, 49, 600-602 (1965) 4. Bunt, J. S. and C. C. Lee. Observations within and beneath antarctic sea ice in McMurdo Sound and the Weddell Sea. Methods and Data. IMAS Special Publ.,~Univ. of Miami, 69-1 (1969). 5. Bunt, J. S. and C, C. Lee. Seasonal primary production within and beneath antarctic sea ice. J. Mar. Res., 2(3), 304-320 (1970) 6. Bunt, J. S., K. E. Cooksey, M. Reeb, C. C. Lee and B. F. Taylor. Algal nitrogen fixation in the marine subtropics: Assay by acetylene reduction. Nature, 227, 1163 (1970). 7. Corcoran, E. F. and C. C. Lee. The distribution of chlorinated hydrocarbon pesticides in the Mississippi River Delta and the adjacent Gulf of Mexico. Joint Southeast and Southwest ACS Meeting, New Orleans (Abst.). Dec. 2-4, 1970. p. 88. 8. Taylor, D. L. and C. C. Lee. A new Cryptomonad from Antarctica: Crvptomonas cryophila sp. Nov. Archiv fur Mikrobiol., 75, 269-280 (lJVl). 9. Bunt, J. S., C. C. Lee and E. L. Lee. Primary productivity and related data from tropical and subtropical marine sediments. Mar. Biol., 16, 28-36 (1972). 10. Bunt, J. S. and C. C. Lee. Data on the composition and dark survival of four marine microalgae. Limnology and Oceanogr., 17, 458-461 (1972). 11. Fenchel, T. and C. C. Lee. Studies on ciliates associated with sea ice from Antarctica. I. The nature of the fauna. Archiv fur Protistenk., 114, 231-236 (1972). 12. C. C. Lee and T. Fenchel. Studies on ciliates associated with sea ice from Antarctica. II. Temperature responses and tolerances in comparison with ciliates from temperate and tropical habitats. Archiv fur Protistenk., 114, 237-244 (1972). HONS 009163 1 PCJBLICATIONS CONT'D.V ) 13. Bunt, J. S., C C Lee and M. A. Heeb. The site off Freeport Intended Cor Hydro-Lab. Hydro Lab J., 1^3-6 (1972). 14. ' Bunt, J. S., C. C. Lee. An exploratory study of benthic primary production. Hydro Lab J., 1^7-10 (1972). 15. Taylor, B. F., C. C. Lee and J. S. Bunt. Nitrogen-fixation associated with the blue-green alga, Trichodesmium, as measured by the acetylene-reduction technique. Archiv fur Mikrobiologie, 88:205-212 (1973). IS. C. C. Lee, E. L. Lee and J. S. Bunt. Distribution of biomass in a coral reef transect. In: Results of the Tektite Program, coral reef invertebrates and plants (Sylvia Earle and Robert Lavenberg, editors), Natural History Museum, Los Angeles County, Science Bull., 20:69-75 (1975). . HONS 009164 () CURRICULUM VITAE July 1977 Theodore J. Kneip ) Dace and Place of Birch: MariCal Status: Residence: December 20, 1926 - SC. Paul, Minnesota Married, Six children 162 E. Fairmount Avenue Maywood, New Jersey 07607 . Professional Address: New York University Medical Canter Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext. 5232 Title: Research Professor, Deputy Director Laboratory for Environmental Studies Education: 1950 - B. of Chemistry (with distinction) University of Minnesota 1952 - Master of Science university of Illinois 1954 - Doctor of Philosophy University of Illinois Positions Held: New York University Medical Center Department of Environmental Medicine 1967 - present Senior Research Scientist 1967 - 1975 Adjunct Associate Professor 1969-1971 Adjunct Professor 1971-1975 Research Professor 1975 - Acting Director, Laboratory for Environmental Studies 1968-1970 Deputy Director, Laboratory for Environmental Studies 1970-present HONS 009165 Curriculum Vitae Page 2 ) Course Offerings: (Department of Environmental Medicine) Analytical Chemistry of Environmental Contaminants. Radiochemistry. Positions Held: (continued) Mallinckrodt Chemical Works St. Louis, Missouri 1954-1966 Research Chemist, Uranium Division 1954-1957 Group Leader, Uranium Division 1957-1959 = Head, Analytical Research Laboratory 1959-1961 Asst. Manager, Analytical Laboratory, 1961-1963 Uranium Division Manager, Laboratory Products Research 1963-1966 Honor Societies: Phi Lambda Upsilon, 1950 Sigma Xi, 1954 Professional Societies: American Chemical Society American' Association for the Advancement of Science American Public Health Association American Industrial Hygiene Association Air Pollution Control Association American Conference of Governmental Industrial Hygienists . The New York Academy of Sciences Vice Chair - Environmental Sciences Section - 1976 Chair - Environmental Sciences Section - 1977 - 78 Elected Fellow - 1977 MQNS 009166 .Curriculum Vitae Page 3 !) Committees: Consulting Chairman of Subcommittee t 6 Metals 1, Intersociety Committee on Manual of Methods for Ambient Air Sampling and Analysis 1971-1977 Member 1971-present Member of International Meeting on Effects and Dose-Response Relationships of Toxic Metals, Tokyo, November 18-23, 1974. Organized by: The Subcommittee on Toxicology of Metals of the Permanent Commission and International Association on Occupational Health. Member of American Society for Testing and Materials Committee D-22: Methods of Sampling and Analysis of Atmospheres, Subcommittees D-22.04, 1973-Present. D-22.09, 1975-Present. New York State - Governor's ad hoc committee on PCB's, 1975. Secretariat, International Standards Organization/Technical Committee 146 (Air Quality)/MOrking Group 3 Nitrogen Compounds, 1975-1976. Secretariat, International Standards Organization/Technical Consaittee 146 (Air Quality)/Subcomaittee 2 Workplace Atmospheres, 1976-present. Various corporate organizations including Consolidated Edison, RSR Corporation, Equitable Environmental Health. United States Department of Commerce, National Oceanic and Atmospheric Administration, Marine Ecosystems Analysis Project, New York Bight Contaminants Panel. Cornell University, New York State College of Agriculture and Life Sciences and -New York State College of Human Ecology, Cooperative Extension, New York City Urban Gardening Program. SI HONS 009167 Curriculum Vitae Page 4 ) List of Published Works: 1. Laitinen, H. A. and T. J. Kneip. "Polarographic and Coulonetric Study of p-Dimethylaminobenzene," Journal of the American Ch-,< Society, 78:736-740 (1956). '--------- 2. King, P., E. Bretz and T. J. Knelp. "Determination of Iodine in Mineral Premixes," Analytical Chemistry, 3_4:565-567 (1962). 3. Knelp, T. J.. T. H. Beasly, P. King and W. K. Dean. "Programmed Temperature Gas Chromatography with Dual Electron Capture Detector for Pesticide Residue Analysis," Analytical Chemistry, 39:1510 1512 (1967). " .-- 4. Cohen, N. and T. J. Knelp. "A Method for the Analysis of 210Pb in Urine of Uranium Miners," Journal Health Physics. 17:125-130 (1969). . 3^ Strehlow, C. D. and T. J. Knelp. "The Distribution of Lead * and Zinc in the Human Skeleton," Journal American Industrial Hygiene Association, 30:372-378 (1969). . 6. ' Knein, T. J., G. *P. Howells and M. E. Wrenn. "Trace Metals, JUBlOnuellffSs and Pesticides in the Hudson River," Symposium on Hudson River Ecology, October 1969, Proceedings of the Symposium, New York State Department of Environmental Conservation,"Albany, Mew York. . 7. Purtado, V. C., T. J. Knelp and M. Eisenbud. "Measurement of Low ' Levels of 13iI in Reactor Atmospheres," Nuclear Applications and Technology, 9:268-273 (1970). B. Kneip, T. J., M. Eisenbud, C. D. Strehlow and P. C. Freudenthal. "Almorne particulates in Ne; York City," Journal Air Pollution Control Association, 20:144-149 (1970). - 9. Howells, G. P-. T. J. Kneip and M. Eisenbud. "Water Quality in Industrial Areas: Profile of a River," Environmental Science and Technology, 4:26-35 (1970). X0. xnelp. T. J. "Atomic Abosrption Spectrophotometry," AIHA Confer ence on Advances in Instrumental Method of Analyses, Environmental . Trace Substances Center, University of Missouri, Columbia, Missouri (May, 1970). , HONS 009168 Curriculum Vitae Page 5 11. Laurer, G. n- - t. ,t. Kneln. R. B. Albert and F. S. Kent. 'In Situ Determination of Lead on Painted Surfaces for the Prevention of Childhood Lead Poisoning.* Proceedings of the Third Svmoosiun on Low Enerby x- and Ga-tma Rav Sources and Applications .Boston. Gordon and Breach Science Publishers, New York, pp. 289-ioi (1971). 12. Lentsch, J. W., M. E. Wrenn, T. J. Knelp and M. Eisenbud. "Manmade Radionuclides in the Hudson River Estuary,* Proc. 5th Annual Midyear Topical Symposium, Health Physics Society, Idaho Falls, Idaho, p. 499 (1970). 13. Howells, G. P., M. Eisenbud and T. J. Knelp. "Ecology of the Estuary of the Lower Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and Its Effects on Living Resources and Fishing. Publ. FIR:M?/70/E-20, Food and Agriculture Organization of the United Nations, New York (1970). 14. . Knelp. T. J. "Concentration Factors, Environmental Complexity and the Analytical Chemist," American Chemical Society,'Analytical Division--National Bureau of Standards Summer Symposium: Key to Progress in National Problem Areas, Washington, D. C. June 1971, Proceedings of the Symposium 15. Knelp. T. J.. G. P. Hovells and M. E. Wrenn. "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources and Fishing, Publ. FIR:MP/70yE-20. Food ami Agriculture Organization of the United Nations, New York (1970). 16. \ Laurer, G. R., T. J. Kneip, R. E. Albert and F. S. Xent. "X-ray Flourescence: Detection of Lead in Wall Paint," Science. 172: 466-468 (1971). ---------------- ------- 17. ' Knelp, T, J., N. Cohen, D. H. Goldstein and E. A. S. Kuchmore, "Experimental Studies of Lead Toxicity in Trace Substances in Environmental Health - V," Proceedings of University of Missouri's 5th Annual Conference on Trace Substances in' Environmental Health (ed. by D. D. Hemphill), pp. 349-361. ( June 1971) . 18. Eisenbud, M. and T. J. Knelp. 'Trace Metals in the Atmosphere," Tech. Paper.#16, New York State Department of Environmental Con servation, Environmental Quality Research and Developaient Unit (July, 1971). 19. Lentsch, J. W.. T. J. Knelp. M. E. Wrenn, G. P. Howells and M. Eisenbud. "Stable Manganese and Manganese-54 Distributions in ' The Physical and Biological Components of the Hudson River Estuary," Proc. Third National Symposium on P.adloecologv, Oak Ridge (1971). HONS 009169 e* * Curriculum Vitae Page 6 i ) 20. Kneip, T. J. `Sampling and Analytical Problems in a Complex Natural System: The Hudson River Estuary,* American Laboratory 3^47-52 (1971). 21. Cohen, N., T, J. Kneip, D. Goldstein and E.A.S. Muchmore, "The Juvenile Baboon as a Model for Studies of Lead Poisoning in Children,* Journal of Medical Primatology 1: 142-155 (1972). " 22. Kneip, T. J. and G. R. Laurer. 'Isotope Excited X-ray Fluorescence,* Analytical Chemistry, 44iS7A-66A (1972). 23. Laurer, G., M. Eisenbud, R. E. Albert, T. J. Kneip and N. Nelson. *New York City Health Research Council - X-ray Fluorescence Analyzer for Lead,* Isotopes and ' Radiation Technology 9:275-277 (1972). *24. Kneip. T. J.. et al., "Tentative Method of Analysis for Inorganic Lead Content of the Atmosphere, * Health Laboratory Science 9(1) :79-83 (1972) . 25. Laurer, G. R., R. E. Albert, T. J. Kneip, B. Pasternack, C. Strehlow, N. Nelson and F. S. Kent, ''The Distribution of Lead Paint in New York City Tenement Buildings,' AJPH 63: 163-168 (1973). --------- 26. Kneip, T. J. and G. J. Lauer. "Trace Metal Concentration Factors in Aquatic Ecosystems,* in Vol. 5, Progress in Analytical Chemistry, Plenum Press, New York, New York (1973). 27. Ahuja, S., E. M. Cohen, T. J. Kneip, J. L. Lambert, G. Ziweig, eds. Chemical Analysis of the Environment and Other Modern Techniques. Plenum Press. New York. New York (1973). 28. ^9. Kleinman, M. T., T. J. Kneip and M. Eisenbud, 'Meteorological Influences on Airborne Trace Metals and Suspended Particulates,* Proceedings, 7th Annual Conference on Trace Substances in Environmental Health. Dnivarsity of Missouri, Columbia, Missouri, June 12-14 (1973). Kneln. T. J., et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter," Health Laboratory Science 1(3) :214-217 (1973) . *30. Kneip. T. J.. et al., "Tentative Method of Analysis for Molybdenum Content of Atmospheric Particulate Matter by Atomic Absorption Spectrophotometry," Health Laboratory Science 10(3) :218-220 (1973). --------------- *Reprinted in Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D.C. (1977). MONS 009170 Curriculum Vitae Page 7 ) *31. Kneip. T. J., at al., "Tentative Method of Analysis for Nickel Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(3) :221-225 (1973). ! ---------------- *32. Kneip, T. J., et al., "Tentative Method of Analysis for Copper Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(3):226-230 (1973). *33. Kneip, T. J., et al., "Tentative Method of Analysis for Chromium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(4):358-361 (1973). ------------------- 34. Kneip, T. J., G. P. Howells, M. E. Wrenn, "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," In: Marine Pollution and Sea Life. Fishing News (Books) Ltd., Surrey, England, p. 169 (1973). (Reprinted from Ref. 15) 35. Kneip, T. J., M. T. Kleinman and M. Eisenbud, "Relative Contributions of Emission Sources to the Total Airborne Particulates in New York City," Proceedings, Third ' International Clean Air Congress, October 8-12. 1973 (1974). 36. Cohen, N.,T. J. Kneip, V. Rulon and D. Goldstein, "Biological and Toxicological Response of Infant Baboons to Lead Driers in Paint," Env. Hlth. Perspectives, Experimental Issue #7, DHEW PubTTNl3~74-2l8, U.S. Gov't Printing Office, Washington, D.C., p. 161 (1974). 37. Kneip, T. J., N. Cohen and V. Rulon, "Potential Sources . of Analytical Error in the Erythrocytic 6-Aminolevulinic Acid Dehydratase Test for Lead Exposure," Analytical Chemistry, 46:1863-1866, (1974). ---------------------- 3* Bernstein, D. M., T. J. Kneip. M. T. Kleinman, R. Riddick and M. Eisenbud, "Uptake and Distribution of Airborne Trace Metals in Man," Proceedings of the 8th Annual Conference of Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri, June (1974) . 39. Kneip, T. J., G. R& and T. Hernandez, "Cadmium in an Aquatic Ecosystem: Distribution and Effects," Trace Substances in Environmental Health - VIII, 1974'.' A Symposium! D. D. Hemphill, ed.,"University o" Missouri, Columbia (1974) . HONS 009171 2 Curriculum Vitae Page 8 ) 40. Bondietti, E. A., F. H. Sweeton, T. Tamura, R. M. Perhac, L. D. Nulett, and T. J. Kneip. "Characterization of Cadmium and Nickel Contaminated Sediments from Foundry Cove, New York," In: Proceedings of the First Annual NSF-RANN Trace Contaminants Conference, Oak Ridge, Tennessee, August, 1973. pp. 211-224. CONF-7308ii, ORNL, Oak Ridge, Tennessee (1974). *41. Kneip, T. J., et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health laboratory Science 11(2):112-116 (1974). *42. Kneip, T. J., et al., "Tentative Method of Analysis for Iron Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 118-121 (1974). *43, Kneip, T. J., et al., "Tentative Method of Analysis for Lead content of. Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 122-127 (1974) . *44. ' Kneip, T. J., et al., "Tentative Method of Analysis for Manganese content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2):128-133 (1974). ---------------- *45. Kneip, T. J., et al., "Tentative Method of Analysis for Zinc Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 134-139 (1974). *46. Kneip, T. J., et al., "Tentative Method of Analysis for Vanadium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(3) :240-245 (1974). -------------------* *47. Kneip, T. J., et al. Tentative Method of Analysis for Elemental Mercury in Ambient Air by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 11(4) :342-346 (1974). HONS 009172 `Curriculum Vitae Page 9 (.) *48. ?SiE' T" J-' M* isenbu<i# **. T. Kleinman and D. M. Bernstein, "Studies of Trace Substances in an Urban Atmosphere," Proceedings of the International Symposium of Recent Xdvances in the Assessment of the Health Etiects or Environmental Pollution, Paris, June 24-28. 1974 (1975). *49. Goldstein, D. H-, T. J. Kneip, V. Rulon and K. Cohen, "Erythrocytic 4-Aminolevulinic Acid Dehydratase (i-ALAD) Activity as a Biological Parameter for Determining Exposures to Lead," Journal of Occupational Medicine, 17:157-162 (1975). 50 Kneip, T. J., "Potential Applications of X-ray Analytical ' Method in Paint Analysis,"' Journal of Paint Technology, 47:71-76 (1975). 51. Kneip, I. J., T. Hernandez and Gary R6, "Cadmium in an Aquatic Ecosystem: Transport and Distribution," In: Trace Contaminants in the Environment," Proceedings of the Second Annual NSF-RANN Trace Contaminants Conference, Asilomar, Pacific Grove, California, 29-31 August, 19^4 (1975) 52. Kneip, T. J., et al., "Tentative Method of Analysis for Elemental Mercury in the Working Environment by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 12(2):158-162 (1975). 53. Kneip, T. J., et al., "Lead in Blood and Urine," Health Laboratory Science 12^(4) :375-378 (1975) . ' 54. Kneip, T. J., et al., "Mercury in Urine," Health Laboratory Science 12(4):379-382 (1975). 55. Kneip, T. J., et al., "General Atomic Absorption Procedure lor Trace Metals in Airborne Material Collected on Membrane Filters," Health Laboratory Science 12(4):383-393 (1975). 56. Kneip, T.J., N. Cohen, V. Rulon, David Goldstein and N. Nelson, "Factors Influencing Dose-Response Relationships for Toxic Metals," In: Effects and Dose-Response Relationships of Toxic Metals, G.F. Hordberg, ed., Associated Scientific Publishers, Amsterdam (1976). 57.Eisenbud, M., T. J. Kneip, M. T. Kleinman, D. M. Bernstein, "Trace Metals in Urban Aerosols," Final Report to Electric Power Research Institute, October, 1975. NTIS Pb-248-324 (1976). HUNS 009173 Curriculum Vitae Page 10 ) 58. Bernstein, D. M., M. T. Kleinman, T. J. Kneip, T. L. Chan and M. Lippmann, "A High-Volume Sampler fortha Determination of Particle Size Distributions in Ambient Air,* Journal of the American Air Pollution Control Association 26(11). TC6?rT072 (1976). 59. Kleinman, M. T., T. J. Kneip and H. Eisenbud, 'Seasonal Patterns of Airborne Particulate Concentrations in New York City," Atmospheric Environment 10_:9-11 (1976). SO- Kleinman, M. T., D. M. Bernstein and T. J. Kneip, "An Apparent Effect of the Oil Embargo on Total Suspended Particulate Matter and Vanadium of New York City Air," J. Air Poll. Con. Assoc. 27_:65-67 (1977). ' 61. * Kneip, T.J., R. Peter Mallon and Virginia P. Rulon, "TheEffects of Anemia on Heme Synthesis Parameters During Lead Exposure," American Industrial Hygiene Association Journal 37, 578 -585 (1977). 62. Kneip, T. J.. et al., "Analytical Method for Antimony in Air and Urine," Ins Methods of Air Sampling and Analysis, Second edition, Xatz, M., ed. American Public Health Association, Washington, D. C., pp. 765-769 (1977). 63. Kneip, T. J., et al., "Analytical Method for Arsenic in Urine and Air," In: Methods of Air Sampling and Analysis. Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 769-773 (1977). 64. Kneip, T. J., et al., "Analytical Method for Arsenic, Selenium and Antimony in Urine and Air by Hydride Generation and Atomic Absorption Spectroscopy," In: Methods of Air Sampling and Analysis. Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 774-780 (1977). ' 65. Kneip. T. J.. et al., "Analytical Method for Chromic Acid Mist in Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public HealtK Association, Washington, D. C., pp. 785-788 (1977). 66. Kneip, T. J., et al., "Analytical Method for Lead in Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 808-818 (1977). 67. Hazen, R. E. and T. J. Kneip, "The Distribution of Cadmium in the Sediments of foundry Cove," In: Hudson River Ecology, Proceeding of a Symposium, Hudson River Environmental Society, ed. March 29-30, 1976 (1977). HONS 009174 Curriculum Vitae Page 11 V> ) 68. Kneip, T. J., M. T. Kleinman, R. Riddick and D. M. Bernstein. "Trace Elements in Human Tissues," Proceedings of the International Workshop on Biological Specimen Collections. April J.S-22, i.'i 11, Luxembourg, Impress, 1977). 69. Kneip, T. J., "Effects of Cadmium in an Aquatic Environment," Ins Cadmium 77, Edited Proceedings first International Cadmium Conference, San rrancisco, 51 Jan.-2 Feb.. 1977. Metal Bulletin Limited, London, pp. 120-124, (1978). 70. Daisey, J. M., I. Hawryluk, T. J. Kneip and F. Kukai, "Mutagenic Activity in Organic Fractions of Airborne Particulate Matter," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 71. Kneip, T. J., M. A. Leyko, M. T. Kleinman, M. Lippmann and ` J. M. Daisey, "Organic Matter in Hew York City TSP: Comparisons of Seasonal Variations and Relationships to Source Tracers," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 72. Lioy, P. J., G. T. HOlff and T. J. Kneip, "Toxic Airborne Elements in the New York Metropolitan Area," J. Air Poll. Con. Assn., 28(5)s510-512 (1978). 73. tenderer, B. P., D. M. Bernstein, J. M. Daisey, M. T, Kleinman, T. J. Kneip, E. O. Knutson, M. Lippmann, p. j. Lioy, K. A. Rahn, D. Sinclair, R. L. Tanner, and G. T. Wolff, "Summary of the Hew York Summer Aerosol Study (NYSAS)," J. Air Poll. Con. Assn., 28 (4)s321-327 (1978). 74. Fennikoh, K. B., R. I. Hirshfield and T. J. Kneip, "Cadmium Toxicity in Planktonic Organisms of a Freshwater Web," Environ. Res. (In press, June, 1978). 75. Xneip, T. J. and M. T. Kleinman, "Sampling and Multielement Analysis of Ambient Aerosols," In: Special Environmental Report Ho. 10, Air Pollution Measurement Techniques, Proceedings of the WMO Air Pollution Measurement Techniques Conference (APOMET), Gothenburg, 11-15 October, 1976. World Meteorological Organization, Geneva, Switzerland (1977). 76. Laurer, G. R. and T. J. Kneip, "Portable X-ray Units for Analyzing Lead in Paint," In: A Report of the Committee on Environmental Improvement: Assessing the Problem of Lead-Based Paint Hazards, pp. 13-17. American Chemical Society. Washington, D.C. (August, 1978). HONS 009175 f ) PERSONAL EDUCATION HONORS AWARDS SOCIETIES AFFLILIATIONS CURRICULUM VITAE JOSEPH MARTIN O'CONNOR Born: November 19, 1944, Boston, Massachusetts Married: 2 children Bachelor of Science, Biology June, 1966 College of the Holy Cross Worcester, Massachusetts (Philosophy minor) Doctor of Philosophy, Ecology/Physiology November, 1971 State University of New York at Albany Albany, New York Dissertation Title: Photoperiodic control of pituitary gonadotropin release in trout (Salmo gairdneri Richardson and Salvellnus fontinalls (Mitchell) and in the leopard frog fRana pipiens Shreber) Sigma Xi Beta Beta Beta Biological Society Student representative to Faculty Senate State University of New York at Albany R.E.S.A. (Sigma Xi) Research Grant-in-Aid N.S.F. Pre-doctoral fellowship (Honorable) Research Grant-in-Aid,' Edmund Niles Hyuck Preserve Fellowship, Research Foundation of New York State New York Academy of Sciences American Society of Limnology and Oceanography Estuarine Research Federation Atlantic Estuarine Research Society AAAS American Society of Natural History Ecological Society of America American Fisheries Society Sigma Xi Great Lakes Research Society Florida Defenders of the Environment Board of Consultants, Lawler, Matusky and Skelly Engineers, Tappan, New York HONS 009176 experience present 1974-75 1973-74 1970-73 1967-70 1965-67 (stumers) PROFESSIONAL ACTIVITIES o) Research Assistant Professor Department of Environmental Medicine, and Assistant Director - Laboratory for Environmental Studies New York University Medical Center Sterling Forest, Tuxedo, New York Senior Biologist Office of Professional and Technological Development Lawler, Matusky and Skelly Engineers 415 Rt. 303, Tappan, New York Director Biological Studies Section and \ Laboratory Office of Research and Technology Quirk, Lawler and Matusky Engineers and QLM Laboratories, Inc. Tappan, New York Research Associate University of Maryland Natural Resources Institute Hallowing Point Field Station Prince Frederick, Maryland Graduate Research Fellow Dept, of Biological Sciences State University of New York at Albany Faculty Research Assistant University of Maryland' Natural Resources Institute Chesapeake Biological Laboratory Solomons, Maryland Member, Advisory Committee Chesapeake Bay R.A.N.N. Program of the National Science Foundation 1971-1972 U.S. Dept, of Agriculture Graduate School Programs in Aquatic Ecology and Pollution Biology Counselor Rockland County Guidance Center for Women Guidance in Career Programs Adjunct Assistant Professor Inst, of Mar. and Atmos. Sciences of the City University of New York HONS 009177 7 )3 Advisor, Fisheries Biology U.S. Dept, of Commerce National Oceanic and Atmospheric Administration New England Offshore Mining Environmental study 1972-1974 Visiting Investigator University of Delaware College of Marine Studies Bayside Laboratory Lewes, Delaware 1972 RESEARCH INTERESTS Population dynamics Community dynamics Respiratory physiology of fishes Reproductive physiology of fishes Chemical-biological interrelationships in ecology Ethology of fishes TEACHING INTERESTS Physiological ecology General physiology General ecology Pollution biology Ichthyology PUBLICATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of frog ovarian oocytes. Amer. Zool. 9:549. O'Connor, J.M. and R.G. Stross. 1970. Photoperiodic control of the pituitary gonadotropin release patter of the frog, Rana plpiens Shreber. Abstr. Proc. N.E. Division Comp, physiology section, Amer. Soc. Zool. O'Connor, J.M. 1970. The effects of thermal shock on the tidal activity patterns of the hogchoker, Trinectes maculatus. Abstr. Amer. Society of Ichthyologists and Herpetologists, Ann. Mtg., New Orleans, La. O'Connor, J.M. 1971. Photoperiodic control of the pituitary gonadotropin release patterns in the rainbow trout (Salmo cairdneri (Rich.,)), the brook trout (Salvelinus fontinalis Mitchell) and in the leopard frog (Rana pipiens Shreber). Ph.D. Thesis. Library, State University of New York at Albany, and University Microfilms, Ann Arbor, Mich. HONS 009178 o 4 ) O'Connor, J.M. 1972. Pituitary gonadotropin raleasa patterns in rainbow trout (Salmo qairdneri) brook trout (Salvelinus fontinalia) and in the leopard frog (Rana pipiens). Comp. Biochem. Physiol. 43(A):739-746. O'Connor, J.M. 1972. Tidal rhythm in the activity patterns of the hogchoker, Trinectes maculatus (Bloch and Schneider). Bxp. Mar. Biol. Ecol. 9:1^3-178. Shark, J.A., J.M. O'Connor and D.A. Neumann. 1972. The effects of suspended and deposited sediments on estuarine organisms. Oniv. Md. N.R.I. ref. 72-9. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1973. The effects of suspended and deposited sediments.on estuarine organisms. Univ. Md. N.R.l. ref. no. 73-4. O'Connor, J.M., R.N. Williams and j. Converse. 1974. Studies of fish impingement at the Nine Mile Point Nuclear Station, Lake Ontario. Abstr. 17th Conference on Great Lakes Re search. int'l Assn, for Great Lakes Research. O'Connor, J.M. and J.A. Sherk. 1974. The responses of some estuarine organisms to concentrations of suspended solids. Proc. of the Seventh Dredging Seminar, Ctr. for Dredging Studies, Texas A&M University. Sherk, J.A., J.M. O'Connor and D. Neumann. 1975. The effects of suspended sediments on estuarine environments. IN: L.E. Cronin (Ed.). Estuarine Research, Vol. II. Geology and Engineering, Academic Press, H.Y. pp. 541-557. Neumann, D.A., J.M. O'Connor and J.A. Sherk. 1975. Respiratory and hematological responses of the oystertoadfish (Opsanus tau) to suspended sediments. Trans. Amer. Fish. Soc. 131:775-781. Sherk, J.A. and J.M. O'Connor. 1976. Effects of sedimentation on coastal zone organisms. Proc. Ill Federal Inter-Agency Sedimentation Conference. O'Connor, J.M. and S. Schaffer. 1977. gear on striped bass ichthyoplankton. 18:312-315. Effects of sampling Chesapeake Science Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1976. Effects of suspended solids on selected estuarine plankton. O.S. Army corps of Engineers Coastal Engineering Research Center. Misc. Rept. 76-1. 50 pp. Jan. 1976. MOMS 009179 l 5 ) O'Connor, J.M., D.A. Neumann, and J.A. Sherk, Jr. 1976. Lethal effects of suspended sediments on estuarine fish. Technical Paper No. 76-20, O.S. Army, Coastal Engineering Research Center, 38 pp. O'Connor, J.M., D.A. Neumann, and J.A. sherk. 1977. sublethal effects of suspended sediments on estuarine fish. Technical Paper No. 77-3, U.S. Army, Coastal Engineering Research Center, 90 pp. ' Rachlin, J., A.P. Beck, and J.M. O'Connor. 1978. Karyotypic analysis of Hudson River striped bass. Morone saxatilis (Halbaum). Copeia 1978(2):334-336. Ginn, T.C. and J.M. O'Connor. 1978. Responses of the estu arine amphipod Gammarus daiberi to chlorinated power plant effluent. Estuarine Coastal Mar. Sci. 6:459-469. . Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1978. An opera tional system for simulating the physical and chemical en vironment of the condenser tubes of a power plant. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). Zubarik, L.S., and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). O'Connor, J.M., C.C. Lee, and M. Eisenbud. Environmental Health. M.E.S.A. New York Bight Atlas Monograph 32 (in press). . Ginn, T.C. , G.V. Poje, and J.M. O'Connor.. 1978. Survival of planktonic organisms following .passage through a simu lated power plant condenser tube. Proc. Fourth Nat'l Workshop on Entrainment and Impingement, pp. 91-101. PUBLICATIONS IN REVIEW O'Connor, J.M., D.A. Neumann and J.A. Sherk. The responses of estuarine fishes to highly turbid environments. Water Res O'Connor, J.M. and J.A. Sherk. The effects of suspended mineral solids on the white perch. Morone americana. Trans. Amer. Fish. Soc. O'Connor, J.M. and J.A. Mihursky. The effects of thermal shock on the activity patterns of the hogchoker, Trinectes maculatus. Ches. Sci. HONS 009180 o6 MANUSCRIPTS IN PREPARATION Eichorn, K.L., T.C. Ginn and J.M. O'Connor. Response of striped bass (Morone saxatills) to chlorinated cooling water. O'Connor, J.M., G.V. Poje, and S. Rigdon. Respiration of Striped bass (Morons saxatills) eggs and larvae. Becker, A.J. and J.M. O'Connor. Aerobic shutdown in the green crab (Carcinus maenas) in response to temperature, salinity and sulfide. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor and M. Wrenn. Effect of high LET radiation on intracellular ATP content of prokaryotic and eukaryotic algae. Hendrick, E., J.M. O'Connor, and G.R. Laurer. A method for analysis of trace mineral content of fish otoliths using X-ray fluorescence spectroscopy. PRESENTATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of ovarian oocytes in the frog, Rana piplens. Amer. soc. zool. O'Connor, J.M. and R.G. Stross. 1969. Photoperiodic control of the pattern of pituitary gonadotropin release in the frog Rana piplens Shreber. Amer. Soc. Zool. O'Connor, J.M. 1966. Alterations in the tidal activity rhythm in the hogchoker after exposure to thermal shock. Atlantic Estuarine Research Society, Cambridge, Md. October, 1966. O'.Connor, J.M. and J.A. Shark. 1971. The lethal effects of suspended solids on estuarine fishes. Joint Mtg. Atlantic Estuarine Research Society and the Estuarine Research Federation. Pleasantville, N.Y. October, 1971. Neumann, D.A., J.M. O'Connor and J. Sherk. 1972. Studies of the respiration of the striped bass and the white perch exposed to suspended sediments. Atlantic Estuarine Research Soc. Ocean City, Md. April, 1972. O'Connor, J.M., J.A. Sherk and D. Neumann. 1973. 'Inert1' mineral compounds as potential factors in mortality and toxin transport in estuarine organisms. E.P.A. Workshop on Chemical Pathogens in the Environment. Pensacola, Fla., May, 1973. HONS 009181 l )7 Sherk, J.A., J.M. O'Connor and D. Neumann. 1973. The effects of suspended solids on estuarine environments. Symp. on Recent Advances in Estuarine Research. II Int'l. Conference of the Estuarine Research Federation. Myrtle Beach, S.C. October, 1973. O'Connor, J.M., and J.A. Sherk. 1974. The responses of some estuarine organisms to concentrations of suspended solids. Seventh Dredging Seminar. Sp. by the Center for Dredging Studies, Texas ASM University, New Orleans, La. November, 1974. O'Connor, J.M., R. Williams and J. Converse. 1974. Impinge ment studies at the nine mile point nuclear power station. Lake Ontario. 17th Conference on Great Lakes Research, August, 1974. Hamilton, Ontario, Canada. O'Connor, J.M., and S.G. O'Connor. 1975. Seasonal Compartmentali- zation of primary productivity in the New York Bight, 1973-74. Am. Soc. of Limnology and Oceanography, Halifax, N.S., Canada, June, 1975. ' O'Connor, J.M. 1976. Assessment of entrainment impact at the Indian Point Nuclear Station. Third National Work shop on Entrainment and Impingement, New York City. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tlgrlnus: an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society. May 1976. Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of the striped bass Morone saxatilis and the amphipod Gamaarus to chlorinated power plant effluent. Northeast Fish and Wild life Conference. April 26-29, Hershey, Pennsylvania. Poje, G.V., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrain ment effects. Northeast Fish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1977. An operational system for simulating the physical and chemical environment of the condenser tubes of a power plant. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. Zubarik, L.S., and J.M. O'Connor. 1977. A radioisotopic study of mercury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. MONS 009182 I I8 O'Connor/ Coutant, and J.P. Lawler. 1977. Environ mental impact assessment as ecological research. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. O'Connor, J.M., T.C. Ginn, and G.V. Poje. 1978. Survival of planktonic organisms following passage through a simulated power plant condenser tube. Fourth Nat'l Workshop on En trainment and Impingement. Chicago, Illinois. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor, and H. Wrenn. 1978. Effect of high LET radiation on intra cellular ATP content of prokaryotic and eukaryotic algae. Radiation Research Society. Toronto, Ontario, Canada. HONS 009183