Document 3QnQZwd7qJQv1knL6pmr9g3BD

rt* Aa ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE________________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3017 produced in Antwerp, Belgium. Current information indicates FC3017 is a mixture of 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:_________________________________________________________ Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1995 Species: Daphnia magna Supplier: In-house cultures, maintained at AScl-DETD, Duluth, MN Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: LC50 values calculated using a computer program. Test conditions: Dilution water: Dechlorinated City of Duluth, MN tap water post carbon filter, aerated for 24 hours prior to use. Dilution water chemistry: Hardness: 50 mg/L as CaC0 3 Alkalinity: 46 mg/L as CaC0 3 Conductivity: 127 - 143 pmhos/cm pH: 7.82 TOC: 1.6 mg/L Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis and mixed by mechanical stirring at ambient lab temperature. 002816 Exposure vessels: 250 mL beakers with 200 mL test solution. Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: one plus a blank control Water chemistry during the study: pH range (0-48 hours): 7.9 - 8.2 (control exposure) 7.8 - 7.9 (1000 mg/L exposure) Temperature range (0-48 hours): 20.1 - 20.6 C Conductivity range (0-48 hours): 1 2 7 - 1 3 9 pmhos/cm (control exposure) 1 4 2 - 1 4 3 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48 hours): 8.1 - 8.8 mg/L (control exposure) 7.7 - 8.9 mg/L (1000 mg/L exposure) RESULTS___________________________________________________ Nominal concentrations: Blank control and 1000 mg/L. Element values: 24-hour EC50 = >1000 mg/L 48-hour EC50 = >1000 mg/L 48-hour NOEC = 1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test substance 48-hour EC50 was determined to be >1000 mg/L. The 48hour No Observed Effect Concentration (NOEC) was 1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES_______________________ !____________________________ 00281 This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number N3188, 1995. OTHER___________________________________________________________ Last changed: 6/26/00. 002818 BEST COPY AVAILABLE AScl Corporation/AScl-Duluth Lnvironntcnul Telling Division A Sc I StuJ> ID * 5010*068 STUDY TITLE ACUTE TOXICITY OF FC-3017 TO DAPHNIA MAGNA DATA STANDARD OECD GUIDELINE 202 STUDY COMPLETED December 23, 1995 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 112 East Second Street Duluth, MN 55805-1701 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068 BEST COPY AVAILABLE AScI Corporation/ASi'l* Duluth Environmental Testing Division ASel Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of FC-3017 Light Water Brand Aqueous Film Forming Foam to Daphnia magna. OECD Guideline 202. 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106." Susan A. Beach, 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Tel No. (612) 778-7452. N3188. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street. Duluth, MN 55805; Tel. No. (218) 722-4040, Joe Dierkes. Joe Amato. Donald Mount. December 21-23, 1995. Alan Mozol. Report Signature: Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. BEST COPY AVAILABLE AScI Coqvualmjv'AScl-Duluth Environmental Testing Division AScI Study ID# 5010-068 2.0 OBJECTIVE(S) To determine the 48-h median effect concentration (ECS0) and 48-h no observable effect concentration (NOEC) of the test substance, FC-3017 Light Water Aqueous Film Forming Foam, for Daphnia magna. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 202 (OECD 1993). Test Substance Test Organisms Dilution Water Exposure rIif-f**-,1BC;4ham;bers 1' ' ^ 3.0 TEST METHODS Properties: Clear amber-colored liquid, speciftc gravity ca. 1.1 (water = 1), miscible with water, and stable. Test concentrations: 0 (dilution water control) and 1000 mg/L. High concentration test solution was prepared by mass addition followed by mechanical stirring at ambient lab temperature. Source: Healthy, less than 24-h old Daphnia magna neonates obtained from AScI stock cultures maintained in water of test dilution water quality. Use in test: Gravid adults isolated in dilution water 24 h before the test initiation. Used 5 organisms/replicate and 4 replicates/test concentration. Handled with a smooth bore pipet with an inside diameter of 6 mm. Did not feed during the test. ; Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 50 mg/L (as CaC03) and a pH of 7.82. Aerated for 24 h prior to using in the test. Type: 250-ml borosilicate glass beakers. Cv5 Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. -. Incubation Observations Endpoint Calculations BEST COPY AVAILABLE ASct Cbrp.iralMin'AScl-Dululh Environment! Testing Division AScI Study ID# 5010-068 Duration: 48 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 20C. Biological: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure chambers were gently agitated). Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry: (1) At lest initiation- total hardness, alkalinity, specific conductivity, pH, and DO, (2) daily- temperature, and (3) at test termination- specific conductivity, pH, and DO. All determinations were made according to APHA methods (1989). Formal calculations not needed due to lack of effect. 4.0 RESULTS Test Substance Toxicity (Pertinent data are in Table I) * "' 24-h E C ^ > 1000 mg/L. 48-h ECm: > 1000 mg/L. 48-hNOEC: 1000 mg/L. BEST COPY AVAILABLE AScI Cnrporaijon/AScl-Dululh Environmental Testing Division AScl Study ID* 5010-068 Ranges of Water Chemistry Parameters Total hardness (as CaC03 mg/L): 50. Alkalinity (as CaCOj mg/L): 46. Temperature (C): 20.1-20.6. DO (mg/L): 7.7-8.9. pH: 7.82-8.20. Specific conductivity (umhos/cm): 127-143. 5.0 TEST QA/QC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance (sodium chloride) toxicity test. Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 85% of the air saturation value at 20.0C. The test duration was 48 h. AScl's updated reference toxicity test control chart is in the Raw Data Package. 6.0 REFERENCES American Public Health Association (APHA). 1989. Standard Methods for the Examination of Water and Wastewater.APHA.Washington, D.C. s > V , V ju 1 Organization for Economic Cooperation and Development (OECD). 1981/1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. i Vl,%Spoh*on 3M Company j l y Sponaor Study ID# N31S8 .V .v* 1; i* %,t i , ; mif - -/.L-V5'~AWg* y}- - i-- 'Ve 'V best copy available AMC,, ^ AM.Muib Environmental Testing Division ASet Study ID* 5010-068 TABLE 1. Acute toxicity of FC-3017 Light Water Aqueous Film Forming Foam to Daphnia magna Test Substance Concentration (mg/L) 0 1000 Cumulalis'e Number and % o f Dead and Immobilized Organisms* 24 h 48 b 0(0) 0 (0) 0(0) 0(0) * Percentages are in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS EC and NOEC 24-h EC,, 48-h EC,, 48-h NOEC Data (mg/L) > 1000 mg/L > 1000 mg/L 1000 me/L -- .... ........ - ' "' 95% Confidence Interval Not calculable Not calculable Not applicable 1 |