Document 3QdxrrGee3NZ9qnz2eex0qL5y

STUDY SUMMARY OF ENVIRONMENTAL AFFF AND COMPONENTS - 1991 EFFECTS OF LIGHT WATER BRAND TEST SUBSTANCE Identity:A mixturecontaininpgerfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95,oras a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-203. Currentinformatioinndicateistisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol2,.66 % Sultonefoamer, 3% sodium octylsulfate0,.1% sodium lauryslulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityor degradation of the fluorochemkal component of the testsample. RESULTS Degradability Study BOD5 BOD20 COD BOD5/COD Value(source*) 560 g/L(3M) 717 g/L(GermariArmy Report) 1,060g/L(3M) 1,070g/L(3M) 1,373g/L(German Army Report) -T0.52(3Mand GermanArmy Report Aquatictoxicity Study Microtox EC,o EC5o ActivateSdludgeInhibition Algae (Chloreliapyrenoidosa and Phormidium inundatum) Algae (Scenedesmus subspicatus) 72-hrStatiPcopul.Growth InhibE.CIOT 72-hrStaticPopul.Growth lnhibE.C5o LWater Flea(Daphniamagna) Value(source*) 632 mg/L (German Army Report) 2,500mg/L (German Army Report) None at1,000mg/L (3M) No effecatt<1,000mg/L (3M) 16.9mg/L (German Army Report) 160 mg/L (German Army Report) 24-hrStat@i@cCl-031.6mg/L (German Army Report) 24-hrStaticEC50 430 mg/L (German Army Report) 48-hr StaticEC50 229 mg/L (3M) 48-hr StaticEC50 1,600mg/L (3M) Eastern Oyster Embryo-larvae (CrassostreaVirginica) 48-hrStaticEC50 47 mg/L (3M) Grass Shrimp (Palaemonetes pugio) 96-hrStaticLC5o 510 mg/L (3M) Fathead minnow (Pimephates promelas) 96-hrStaticLC50 750 mg/L (3M) Zebrafish(Brachydanio refio) 48-hrStaticLCo 48-hr StaticLC50 1,053 mg/L (German Army Report) 1,634 mg/L (German Army Report) Scud (Gammarus fasciatus) 48-hrStaticLC5o 1,100 mg/L (3M) Rainbow trout(Oncorhyncus mykiss) 96-hrStaticLC50 96-hr Continuous Flow LI@-,50 1,300mg/L (3M) 400 mg/L (3M) Common mummichog (Fundulus heteroctitus) 96-hrStatiLcC50 2,500 mg/L (3M) Any study marked with "3M" as the source includesstudiesdone at the request of 3M by labs otherthan the 3M Environmental Lab. DATA QUALITY Reliability: These studieshave been assigned Klimishrankings = 4. Values are from a summary lisotnly. OTHER Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 6/27/00 TechnicaRleportSummary Form 6747-11-H To: Patent& TechnicalCommunications Services- 201-2C-12 3M Report Summary must be typewritten.Guidelines are on reverseside.ItreportIsprintedon both sides of paper, send two copies to P & TCS. Document Number: To Pike MT Author($) lp,"ffonlNumber 0222 ProlecNtumber -RQ,mgli@ RD DivislonfDepartment -Envix-ormen,tal--EngirLeer-ing--4-Y-o-llu.t.i-g-rL.-@C-oAt.rQ!.----@ ProjeDcetscription ReportNumber DateTyped 4-1-91 EmploysN*umb4K$) 295864 cc LOC Organization Code 01 _O @_% I EE&PC (PlacaedditionDa.lN.s InAbstracatrea) -EBY-Researr-h--and-D-amel-a PAMort'ntle I Envirorunental Effects of LIGHT WATER Brand AFFF and Components! Keywords LIGHT WATER, aqueous foam, FC-203, FC-206, ethyleneglycol,butylCarbkol,sodium lauryisulateD,uporiol,sodium dodecylsuffates,odium octylsuffateL,-4640,triethariolaminuer,ea,TritonX-305, loamer,flimer,surfactantt,olykriazole, environmentaleffectst,oxicityD,aphnia toxicityM,icmtox, algaltoxicitfyi,shto)Cickyd,egradabliftyb,iochemicaloxygen demand, BOD, chemical oxygen demand, COD. ProjectObjective& Report Abstract 1.To add tothe knowledge base ofenvironmentaleffectsofLIGHT WATER brand aqueous filmfoffninfgoams and the components used inthe products.2.To identiftyoxiccomponents and to correlatecomponent and producttoxicfties. Thistechnicalreportincludesa summary of environmentaleffectsdata on 3M's productsand the components used in them. The data was taken from the literaturaend from previousstudiesby the 3M EnvironmentalLaboratory.The data foreach component and each producthave been evaluatedforcompleteness and accuracy and additionatlesting requirementshave been noted.Where sufficiendtata on the toxicitaynd degradabilkyofindividuaclomponents were availablet,he data were evaluated and recommendations regardinginclusionofthe ooq)onent infutureproductshave been made. An attemptwas made tocorrelatethe toxicitoyf FC-203 to the toxicitoyf itscomponents by takingintoconsiderationthe component concentrationinthe product.While producttoxicitcyould not be conclusivelycorrelatedtothe toxicitaynd concentrationofindividuaclomponents, major contributortso producttoxicitayre believedto be the filrrietrh,e foamer, butylCarbRolTm,and DuponolTm ME (sodium lauryisuffateC)o.mponents likeltyocontributelessto producttoxicitayre ethyleneglycol,tolykriazoles,odium octylsuffatea,nd Di-water.Furthertoxicittyestingofcomponents and product fomv.jlationsshould enable bettercorrelatiobnetween conwnent and producttoxiaies. ReporlType Security JE R & D Research and Development 0 PiLotPlant 0 MANufacturing 0 Management Summary M Open Reportand Summary NotebmkReference informatiLoinaisoninitiats 0 TRP TriporFielRdeport 0 FACtoryExperiment 0 ENGineering 0 ROI Recordof Invention 0 TECH. Service 0 GOVT. Project 0 OTHER 0 ClosedReport-Open Summary 3M Chemical 0 New ChemicalsReported PAgisiry UseformOM toenteirntCohwnicaRlegistry For P & TCS FiknCode Use Only IN- -TNft. ofP&G" P & TCS EditorInitials Contents 1.Purpose 2 liB.ackground ..........................................................2................. IllG.eneralComments 4 IV.Conclusionsand Recommendations 6 V. EnvironmentalEffectsLiteraturSearch 9 1-EthyleneGlycol(RM 3017) ...........................................1.0................ 2.ButylCarbito(lRM 8887) ..............................................1.2................ 3.Duponol(RM 3021);DuponolME Dry Surfactan(tSodiumLaurylSulfate)......1.4. 4.Tolyltdazol(eRM 27513; CorrosionInhibitToorlyltdazolTeechnicalGrade) .....1.6 5.Foamer (FM-4115) ..................................................1.8................ 6.Filmer(FM-3820 orFC-95) ...........................................1.8................ 7.Na-octylsulfa(tReM 32067 orRM 55040) ................................l.g................ 8.Di-water(RD 199) ..................................................2.0................ 9.FC-203 ............................................................2.1................ 10.VW1 60390 (FC-203 withoutTolyltdazolReM 27513) ..i.....................2.3.............. 11.FC-203CE ........................................................2.4................ 12.FC-206CE ........................................................2.5................ 13.FC-203CF ........................................................2.6................ 14.FC-206CF .........................................................26................. 15.L-4640(New Foamer) ..............................................2.6................ 16.Witcolate7093 (RM 45666) .............................................2..7................. 17.Triethanolamine...................................................2.7................ 18.Urea .............................................................2.9................ 19.TritonX-305 ......................................................3.0................ EnvironmentaElffectosfLIGHTWATERTU Aqueous FilmFormingFoam and Components 1.Purpose The purposoefthistudyistoincreas3eM'sknowledgoeftheenvironmenteaflfectosf LIGHT WATERTM aqueous filmformingfoam and the individuaclomponents used to make the product.And, by understandingtheseeffectst,o minimizethem throughformulationchanges. 11.Background Thisstudywas undertakeninresponse totestingdone by the German Army which was reportedin"InvestigatioonfsToxic Effectsand BiologicaDlegmdabilityofFoam Extinguishing Agents inWastewater by Ekkehard lsingT.he report,hereaftereferredtoas the "German Army Report",includeddata for16 firefightinmgaterialso,fwhich two were 3M's FC-203 and FC-206. The 16 firextinguishinaggents were categorizedas: 5 multi-rangefoam agents 6 film-forminfgoam agents 3 proteinfoam agents 1 film-forminfgluo(ine-protefionam agent 1 specialfoam agent fordeep-frypan fires EnvironmentaleffectsconsideredintheGerman Army Report were: 1.Acute toxicittyothe marine photoluminescentbacterium Photobacterium phosphoreum usingthe Microtox*system.The toxicitwyas expressed as EC-10and EC50,however,the exposure timeisnotclearfrom the German Army Report and may have been 10,20,or 30 minutes.Two multi-rangefoam agents were considered "highltyoxic"T.hree multi-rangefoam agents and 2 filmformingagents were found to be "toxic"F.our film-forminga,ll3 proteint,he film-forminfgluodne-proteiangent,and the deep-fryagent were consideredto have "lowtoxicityF"C.-203 and FC-206 were inthe "lowtoxicityc"ategory. 2.Algae cellmultiplicatiionnhibititoenstusingScenedesmus subspicatusas thetest organismwithan exposure timeof72 hours.Toxicitywas expressed as EC ,and EC50.Allfoam agents withtheexceptionoftwo ofthe proteinfoam agents were "toxict"o algae.The two proteinfoam agents had weak toxicittyo algae.Both 3M productswere considered"toxict"oalgae. 3.Acute toxicittyo Daphnia magna afterexposure for24 hours.The toxicitwyas expressed as LC,o and LC50,butthe effectcriteriownas the organisms'lossofability to swim and willbe referredtoas EC 10and EC50 inthisreport.All5 multi-rangefoam agents,allfilm-forminaggents except FC-206, and the deep-fryagent were "toxict"o -2- DaphniaO.ne film-formaignegnt(FC-206)a,ltlhreperoteiangentsa,ndthe film-formfilnugodne-proatgeeinnthad"lowtoxicittoyD"aphniaF.C-203:"toxic"; FC-206:"lowtoxicity". 4.AcutetoxicittoZyebrafis(hBrachydanriaotiaof)teerxposurfeor48 hoursT.he criterwiaas deathandthetoxiciwtays expresseads LC 10andLCSO.Onlytwo multi-ranfgoeamagentwsere"toxitco"fishT.hreemulti-ranfgoeamagentsandtwo film-formaignegntswere"borderltionxeictweatkolxyie".Weaklytoxicw"ere4 film-formaignegnts2,protefionamagentsa,ndthedeep-fraygentO.ne film-forming agentandthefilm-formfilnugodne-proatgeeinntwere"non-toxiFeC.-203and FC-206wereinthe"weakltyoxiecategory. 5. ChemicaOlxygenDemand(COD)and5-dayBiochemicOaxlygenDemand (BOD 5) ofa 3% solutioofnthetesmtateriianlwaterT.he ratioofBOD .toCOD was usedas a ratingguidelinwei,tha BOD ,/CODratioof0.5orlessconsidere"dinsufficient biochemicdaelgradatioTnw"o.multi-ranaggeentsandonefilm-formaignegnt (FC-206w)ereconsidereidnsufficideengtrlaydebdiochemicalTlhye.ratifoorFC-206 was 0.35O.ne multi-ranaggeentandonefilfmorminaggent(FC-203w)ere considere"dprobabliynsuffic7bieecnatu"setheiBrOD,,/CODratiowsere0.55T.he9 remaininaggentswerealslufficiweintththeiBrOD,,.ICOrDatiorsanginfgrom0.60to 0.89. The,datfaromtheGermanArmy Reporftorthetwo3M productasresummarizedinthetable belowT.hedatafortheMicrotoZxe,brafisChO,D, andBOD5testsseem tobe "reasonable" and"self-consisTtheentd"a.taareconsidererdeasonabblecausetheyagreefairwleyllwith datafrompreviou3sM studieasndareconsideresdelf-consisbteecnatusetheFC-206data areroughlhyaltfhatofFC-203. SummaryofGermanArmyReportData Pmduct FC-2D3 Microtox EC,,, EC, 632 2,500 "LowToxw Algae EC,, EC, 16.9 160 "Toxie-- Daphnia LC,, LC, 31.6 430 ITO)de Zebrafish COD Lcio LCSO BOD, BODICOD Ratio 1,063 1,634 -weakly Toxie 41,200 - 21,500 - 0.52 i"nPs=nyr FC-206 2,040 15,DOO "Low Toxicity 16.3 160 'To)de 305 2.000 "Low Toxicity" 2,036 4,086 'Weakly Toxic" 24,600 - 8,700 - 0.35 "Insuffidenr Note:allconcentrationsinmg/L. -3- For thealgaltoxicitdyata,therearetwo importantpointstoconsider.Firstt,he German Army's algae data show essentialleyquivalentoxicitfyorourtwo products.One would expect more toxicitfyorFC-203 than forFC-206, as shown inthe Microtox,Zebrafish,and Daphnia tests.However, highertoxicitbyy FC-203 than by FC-206 may notalways be true.For example, theremay be a component which isparticulartloyxictoalgae,perhaps disrupting the photosynthetipcrocess.Second, our PED forFC-203 indicatesno effectotwo algal speciesatconcentrationusp to 1,000mg/L.While thetwo algalspecieson the PED are not the same as those inthe German Army Report,they are allfreshwaterspeciesand such a largedifferencientoxicitwyouldn'tbe expected.One importantlimitatiwoinththe PED data is thatitdatesfrom 1974 and ithas been impossibletoverifythe exactformulationofthe sample used inthosetests. Forthe Daphnia data inthe German Army Report,the differencbeetween the -FC-206and FC-203 valuesseems largewhen consideringthe watercontentofthe two formulationsA.lso, thereisa considerabledifferencbeetween the PED data.andtheGerman Army data.Our PED has a 48-hrstaticDaphnia magna LC 50of 1,600mg/L,whilethe German Army Report has a 24-hrstaticDaphnia magna EC50 is430 mg/L.The PED data isquiteold(1973)and the formulationofthetestsample cannotbe verifiedT.here isa majordifferencebetween the PED data and the German Army data inthatthe PED data isthe concentrationwhich causes death to50% ofthe Daphnia testpopulation(LC .)and the German Army value isthe concentrationwhich causes 50% ofthetestpopulationtostopswimming (Effective Concentration= EC50).The German Army valueisinfairagreement withrecentresults which show a 48-hrstaticDaphnia magna EC 50of229 mg/L. One thingthatshould be keptinmind when consideringthetoxicitdyata isthatreproducibility and precisionoftoxicittyestscan varyconsiderablyfrom laboratortyo laboratoraynd over time.However, the differencebsetween theolddata(circa1974) and the more recentdata is more likeadyue tochanges informulatiornatherthan changes intestprotocolortestspecies. At any rate,our primaryinteressthouldbe thetoxicitoyfourcurrentproductsand we-should be concerned withhavingcurrentr,eliabldeata on our PEDS. IllG.eneral Comments The followingeneralstatementssummahze thefactorsguidingthisstudy. 1.The primarypurpose of.thenvironmentaleffectdsata isforinternaulse inevaluating formulationsand ingredientasnd not intendedforsubmissiontogovernmental regulatorayuthoritieTsh.erefore,any testingshouldbe done usinglow-cost, non-GLP methods.An exceptionmay be dataforthe new foamer (L-4640)which may need to be submittedforthe European PMN currentluynderway. 1 DraftReportfrom EnviroSystems Laboratorydated March 10,1991.We have asked EnviroSystemsto repeatthe Daphnia testsforFC-203 and VW1 60390. The preliminary resultsfrom EnviroSystemson the restofthesamples were acceptable. -4- 2.The German Army performsitsown testingand no authoritoytherthan the German Army ispresentlyusingthe evaluationscheme used inthe reportcitedabove. Therefore,thereisno need tochoose a laboratoryt,estprotocolo,rtestspecies applicableonlytothe German Army testscheme. Instead,testmethods and species should be chosen so thattestresultscan be used inas many countriesas possible. 3.Where possibled,ata on the environmentaleffectsofcomponents should be obtained from the literaturoerfrom raw materialsuppliersinordertoreduce testingcostsand time. 4.The NationalInstitutoefOccupationalSafetyand Health(NIOSH) terminologyforthe relativteoxicitoyfthe components and theformulationswillbe used inthe discussionoftoxiceffectsW.hile theseterms were notused inthe German Army Report,they are generallyaccepted inthe US. The toxicitaynd correspondingterms are: ConcentrationAcutely ToxictoTest Population NIOSH Terminology :51mg/L 1 - 10 mg/L 10 - 100 mg/L 100 - 1,000 mg/L >1,000 mg/L "HighlyToxic" "ModeratelyToxic" "SlightlTyoxie "PracticalNloyn-Toxie minsignificaTnotxic7itr 5.The LIGHT WATER AFFF formulationsand components consideredare: 1.EthyleneGlycol(RM 3017) 2.ButylCarbitolO(RM 8887) 3.DuponolO ME (RM 3021) 4.Tolyltriazo(lReM 27513) 5.Foamer (FM-4115). 6.Filmer(FM-3820 FC-95) 7. Na-octylsulfa(tReM 32067 or RM 55040) 8. Di-water(RD 199) 9. FC-203 10.VW1 60390 (FC-203 withoutTolyltdazolReM 11.FC-203CE (same as FC-203C) 12.FC-206CE 13.FC-203CF 14. FC-206CF 27513) -5- 15. L-4640 16.Witcolate7093 (RM 45666) 17.Tdethanolamine 18.Urea 19.TritonX-305 Items 1 to 10 are from a lisgtivento EricReiner by Wim Vanneste 3/12/90.Items11 to 13 were giventothe authorby Wim Vanneste 2/5/91.Items14 to16 were given tothe authorby Roger Alm (2/4/91). IV.Conclusions and Recommendations Inordertocorrelattehe toxicitoyfthe producttothetoxicitoyfindividuaclomponents, the component concentrationistaken intoconsiderationI.nthetablebelow,the toxicittyo Daphnia ofthe pure components are giveninthethirdcolumn. The fiftcholumn contains estimatedtoxicitibeassed on productformulationT.his isthetoxicitwyhich would be expected when the component isdilutedinwatertothesame concentratioans inthe product.For example,one would expectthatsincethe toxicitoyfpure butylCarbitol'oisabout 700 mg/L, thetoxicitoyfa solutionmade of35% butylCarbitoloand 65% waterwould have a toxicitoyf about 2,000. Some interestinogbservationscan be made from these numbers. Firste,ven though tolyftdazoliesquitetoxic,itispresentatlow concentrationintheproductand when dilutedin theproductitprobablydoes notcontributseubstantialtloyproducttoxicityH.owever, this analysisdoes notexoneratetolyltdazolaes a major contributotroproducttoxicityS.econd, the lowestnumbers inthe fiftcholumn indicatecomponents which m(ilble the most likely contributortso producttoxicityb:utylCarbito'l,Duponol* ME (dodecylsulfatet)h,e foamer,and thefilmerU.nfortunatelyt,hereisnota singlecomponent which standsout witha number in thefiftcholumn which issubstantialloywerthan allthe rest.Thismay mean thatthereisa synergistiecffectbetween components. -6- SummaryofRecentDaphniaToxiciDtayta Item Name D,,grgaToxw. (m9&) (9 % oonfidencMemaj) FC-203 Formuladon Component Toxidty Based on FormLdation I . Eth lww lycol (RU301A >1,000 20.00% :@5.000 2. ButylCarbitDlo (RM 8887) 706 (523-1.000) 35.00% 2,017 3. Duponol* ME (RM 3021) 2.9(2.3-3.5) 0.10% 2j= 4. Tolyltriamle (RM 27513) 5. Foaffw (FM-4115) 6. Filmor (FM,UM - FO-95) 19(12-25) SS(SO-81) 49(43-56) 0.05% 2.66% 1.34% 38.000 2AN 3,657 7. Na-"IsLdfate (RM 32D67 or RM 55040) 517(400-1,000) 3.00% 17,233 8. Di-vmter (RD 199) >1,000 37.85% >2.642 9. FC-203 229 (150-444) 10. VW160390 (FC-203 wiftutTolyftd=ole) 165 (128-248) MajorContributor to FC-203 Toxidty? Probablynot Probably Probably Probablynot Probably Probably Probablynot NO x x The importanctonclusiofnromtheseDaphniaresultissthata more completesetoftoxicity data isneeded, especiallfyoralgae.The tablebelow summarizes the additionadlata needed tocompletethe environmentaleffectdsatabase on our productsand theiringredients. Additionatlestsare indicatedwithan X. 2 Preliminaryresultfsrom EnviroSystems,March 10,1991. -7- Summary ofAdditionaTlesting Item Name Mkrotox Algae DaphiVa Fish COD BOD 1. Eth loreglycol (Ra 3017) 2. ButylCarbhW* (RM 8887) x x 3. Duponoi* ME (RM 3021) 4. Tolykftmle (RM 27513) S. Foamer (FM-4116) x x x x x x x x x x x x x 6. Filmor x x (FM-3820 - FC-95) 7. Na-octylsulfate x x (RM 32D67 or RM 5W40) x x x a. DI-water (RD 199) 9. FC-203 x x 10. VW160390 (FC-203 x x x x wkhoutTolyMnole) ii. FC-203CE (same as FO-203C) x x 12. FC-2D6CE x x 13. FC-203CF x x x x x x 14. FC-206CF x x x x x x 15. L-" x x 16. Witcolate7093 (RM 45666) x x x x x x 17. Trietkvmlamine 18. Ursa 19. Tdton X-305 x x x x x x TOTAL 12 is 6 7 8 10 Note:BOD tesfinwgillindude 5-,10-,and 20-* msts. V.EnvironmentaElffectLsiteratuSreearch Datafromprevious3M EnvironmentaLlaboratorSytudiesand fromtheliteratuarree summarized inthissection.Toxicitydata noted by "AQUIRE" were obta@inedfrom a search of the USEPA AQUIRE database on Feb. 12, 1991. Includedas partofthe referenceforthe datafromAQUIRE arethedateofpublicatioofntheofiginjaolurnaalrticalned thereview codeestablishebdy a groupofEPA reviewersT.he reviewerhsaverankedtoxicidtaytafrom the literatubrye the followinsgcale: 1.Meets allreviewcriteria. 2.Proceduresgenerallysatisfactory. 3. Insufficieinntsome area ofthetestmethodologyortestreport. 4.Abstractorforeignpaperwithdatainlimitefdormat. The completecontentsofthe AQUIRE databaseforeach chemicalisnotgiveninthisreport because thedatabaseincludesmany obscurespeciesof'littilneteres(topossum shrimp, Indiancatfishs,nakehead catfisho,ystersc,lams,sponges and many others)and datawhich isconsideredby the EPA reviewerstobe ofquestionablvealue. Additionadlegradabilitaynd toxicitdyatahave been takenfromthe Handbook of EnvironmentalData on OrganicChemicalsby KarelVerschueren(NostrandReinhold,New York,1983).These are referencedby page number inthe Handbook followedby thedateof theoriginarleferenceT.here are many cases inwhichthesame data appeared on AQUIRE and:lnVerschuerenbutonlytheAQUIRE referenceisgivenbecause itincludestheEPA qualityratinogf the originarleference. Raw matedalsupplierwsere identififerdomthe3M PRISM databaseand supplierwsere contactedand asked forany environmentadlatathey have.Unfortunateleyn,vironmentaldata was notgenerallyavailablferom raw materialsuppliersA.nd, thesupplierswere slowin sendingthe limiteddata do have. The sourceand reliabiloifttyhe literatudraetawas takenintoconsideratioinnthe environmentalassessment ofthecomponents and therecommendationsforfurthetresting. Report Format* Informatiofnoreach component orformulatioinsorganizedas follows: Formulationorcomponent name and RM number ChemicalAbstractsService(CAS) Number Vendors Degradabilitdyatafrom theliteratuorrefrom previous3M assessments AquaticToxicitdyatafrom theliteratuorrefrom previous3M assessments EnvironmentalAssessment Recommendations forAdditionaTlesting -9- Detailedinformationforcomponents and formulationsare giveninthe same orderas inthe lisatbove. 1. Ethylene Glycol (RM 3017) CAS # 107-21-1 Vendors:Chemtech, Ashland Chemical,Dow Chemical,Texaco Chemical,Olin,Shell011, Union Carbide,TextileChemical,Ashland Chemical,Unocal Degradabift TheoreticaOlxygen Demand (THOD) BOD5 BODIO BOD,5 BOD20 COD BOD,,/COD Ratio BOD2dCOD Ratio 1.29g/g (Verschuerenp 646) 0.440.22g/g (avgof 12 citations from Verschueren p 646) 0.52g/g(Env.Lab.3 Req. #1940) 0.67g/g(Verschuerenp 646; 1953) 1.19g/g(Env.Lab.Req. #1940) 0.92g/g (Verschuerenp 646; 1953) 1.14 g/g (Env.Lab. Req. #1940) 1.00-+0.0g8/g (avg of3 citations from Ve.rschuerenp 646) 1.02g/g(Env.Lab.Req. #1940) 1.240.04.g/(gavgof3 citations from Verschuerenp 646) 1.28g/g(Env.Lab Req. #1940) 0.4(Env.Lab Req. #1940) 0.8(Env.Lab Req. #1940) Toxicilyo Green Algae (Scenedesmus quadricauda) 7-day StaticPopul.Growth Inhib.5 8-daystaticPopul.Growth lnhib. 10,000 mg/L (AQU IRE 2; 1980) 10,000 mg/L (AQU IRE 2;1978) 3 3M EnvironmentalLab work done inDec. 1973. 4 The toxicitdyata are a partiallistinogf38 testson 16 speciesfrom the EPA AQUIRE database. 5 PopulationGrowth InhibitioCnh:ange incellnumber ofalgalspeciesincluding pre-exponentiallagrateeffects. -10- Protozo(aEntosiphsounlcatum) 72-hrStatiPcopulG.rowthInhib. Blue-greenAlgae (Anacystisaeruginosa) B-dayStaticPopul.Growth lnhib. Bacteria(Pseudomonas putida) Algae (Microcysdsaeruginosa) Protozoa(Uronama pardurzi) Water flea(Daphniamagna) 48-hrStaticEC50 24-hrStaticLCso 24-hrStaticLCso 48-hrStaticLCso Brineshrimp (Artemiasalina) 24-hrStaticLCro 24-hrStaticLCso FatheadMinnow (Pimephatespromelas) 24-hrStaticLC50 48-hrStaticLCso 96-hrStaticLC50 96-hrStaticLC50 BluegilSlunfish(Lepomismacrochirus) 96-hr StaticLCO Rainbow Trout(Salmo gairdnen) 96-hrStaticLC50 Common Shrimp (Crangoncrangon) 48-hrRenewal LC50 96-hr Renewal LC5. Goldfish(Carassiusauratus) 96-hrStaticLCso Guppy (Poeciliraeticulata) 7-day Renewal LC50 10,000mg/L (AQU IRE 2;1980) 2,000 mg/L (AQUIRE 2;1978) >10,000 mg/L (Verschuerenp 647) 2,000 mg/L (Verschuerenp 647) >1 0,000mg/L (Verschuerenp 647) >1,000 mg/L (EnviroSystems1991)6 10,000 mg/L (AQU IRE 2; 1977) 10,000mg/L (AQUIRE 3;'l983) 10,000 mg/L (AQU IRE 3; 1983) 20,000mg/L (AQUIRE 2; 1974) 20,000mg/L (AQUIRE 3; 1983) 10,000mg/L (AQUIRE 3; 1983) 10,000mg/L (AQUIRE 3; 1983) 10,000mg/L (AQUIRE 3; 1983) 49,000 mg/L, 53,000 mg/L, & 57,000mg/L (AQUIRE 2; 1983) - 10,000mg/L (AQUIRE 2;1968) 41,000mg/L (AQUIRE 2; 1980) - 100,000mg/L (AQUIRE 2;1974) 50,000mg/L (AQUIRE 2; 1974) 5,000mg/L (AQUIRE 2; 1979) 49,300 mg/L (AQUIRE 3; 1981) 6 Acute Daphnid toxicittyestingdone by EnviroSystems for3M. Test resultsdated March 10, 1991. EnvironmentalAssessment# Ethyleneglycolhas insignificatnotxicitaynd a high BOD/COD ratioE.ven though itis presentinhighconcentrationintheproduct,itprobablydoes notcontributesubstantialtloy producttoxicity. Recommendations forAdditionaTlestinge None. 2. Butyl CarbitolO(RM 8887) CAS # 112-34-5 (DiethyleneGlycolMonobutyl Ether) Synonyms and vendors:ButylDioxito(lCainChemical),Poly-SolvDB (OlinCorporation), GlycolEtherDB (Oxychem), ButylCarbito0l(UnionCarbideCorporation- Unde Div.), ButylDioxhol(ShellOilCompany), Dowanol DB (Dow Chemical),Ektasolve-DB(Eastman Chemical Products,Inc.) D-egradability; THOD BODs BOD,O BOD,R, BOD20 COD BOD.,,/COD Ratio BOD2dCOD Ratio Toxicity: Bacteria(Pseudomonas putida) 2.17g/g (calculated) 0.25(Verschuerenp 524) 0.69g/g(Env.Lab7.Req.#1940) 1.45 g/g(Env.Lab.Req. #1940) 1.61g/g (Env.Lab.Req. #1940) 1.56g/g (Env.Lab.Req. #1940) 2.08(Verschuerenp 524) 1.83g/g (Env.Lab.Req. #1940) 0.12 (Verschuerenp 524) 0.37(Env.Lab.Req. #1940) 0.85(Env.Lab.Req. #1940) 255 mg/L (Verschuerenp 524) 7 3M EnvironmentalLab work done inDec. 1973. 8 The toxicitdyata are a partiallistinogf15 testson 10 speciesfrom the EPA AQUIRE database. -12- GreenAlgae(Scenedesmus quadricauda) 7-dayStatiPcopulG.rowthInhib. 8-dayStaticPopul.Growth lnhib. 1,000mg/L (AQUIRE 2;1980) 1,000mg/L (AQUIRE 2;1978) Blue-greenAlgae (Anacystisaeniginosa) 8-dayStaticPopul.Growth Inhib. 53 mg/L (AQUIRE 2;1978) Protozoa(Entosiphonsulcatum) 72-hrStaticPopul.Growth Inhib. Protozoa(Uronema parduczi) 73 mg/L (AQUIRE 2;1980) 420 mg/L (Verschuerenp 524) Protozoa(Chilomonasparamedum) 48-hrPopul.Growth lnhib. 2,774mg/L (AQUIRE 4;1980) Water Flea(Daphnia magna) 48-hrStaticEC50 24-hrStaticLC50 706 mg/L (EnviroSystems1991) 2,850 mg/L (AQUIRE 2;1977) BluegilSlunfish(Lepomis macrochinis) 96-hrStaticLC50 1,300mg/L (AQUIRE 2; 1977) Goldfish(Carassiusauratus) 24-hrStaticLC50 2,700mg/L (AQUIRE 2;1979) Guppy (Poeciliraeticulata) 7-dayRenewal LC5. 1,150mg/L (AQUIRE 3;1981) Silveride (Leuciscusidus) StaticLC50 1,805mg/L and 2,304mg/L (AQUIRE 3; 1978) InlandSilversid(eMenidiaberyllina) - 96-hrStaticLCso 2,000mg/L'(AQUIRE 2;1977) EnvironmentalAssessment: BOD datashows fairlhyigh degradabilitTyh.e literatudraeta show thismaterialtohave insignificatnotxicittyofishand Daphnia.The two freshwatergreen algaevaluesfrom AQUIRE are consistentand indicattehatbutylCarbitolOispracticalnloyn-toxictogreen algae.There isconsiderablymore toxicitsyhown toblue-greenajgae,however.We should have a sample ofbutylCarbito0ltestedforalgaltoxicittyobe sureofitstoxiceffects. Recommendations forAdditionaTlesting: Algae and Microtox. -13- 3.DuponolOME DrySurfactan(tRM 3021;Sodium LaurylSulfate) CAS # 151-21-(3SodiumDodecylsulfCaHt3e(CH2)11OS03Na) Vendor*Dupont' Degmdabift THOD BOD Toxicily: Green Algae (Chlorellsap) StaticPopul.Growth lnhib. Green Algae (Chlorellvaulgaris) 72-hrStaticGrowth Inhibiti12on Green Algae (Scenedesmus quadricauda) 7-day StaticPopul.Growth lnhib. 8-day Renewal Growth Inhibition FlagellatEeuglenoid(Entosiphonsulcatum) 72-hrStaticPopul.Growth lnhib. Blue-greenAlgae (Anacystiaseniginosa) 8-dayRenewal Popul.Growth Inhib. Bacteria(Pseudomonas putida) CellMultiplicatiIonnhibition Protozoa(Uronema pardurzi) CellMultiplicatiIonnhibition 2.03g/g(calculated) Swisheeo reportsthat"lineaprrimary alkylsulfateshave long been recognizedas extremelyrapidin primarybiodegradationo,ften disappearinginlessthan a dar and citesapproximately100 references which show degradationratesof80 to 100% withina few hoursordays. 50 mg/L (AQUIRE 4;1980) 0.05mg/L (AQUIRE 4) 0.02 mg/L (AQUIRE 2; 1980) 0.02 mg/L (AQUIRE 2; 1978) 40 mg/L (AQUIRE 2;) 7 mg/L (AQUIRE 2; 1978) 290 mg/L (Verschuerenp 793; 1980) 0.75mg/L (Verschuerenp 793;1980) 9 Phone: 302-774-1000.2/15/91:willsend information. 10 Swisher,R. D. SurfactantBiodegradation2.nd ed. Marcel Dekker:New York, 1987,p 466, 807-811. 11 The toxicitdyata are a partiallistinogfthe 409 testson 49 speciesfrom inthe EPA AQUIRE database. 12 Growth InhibitioMne:asurable change inlengthand/orweight. -14- Waterfle(aDaphnimaagna) 48-hSrtatiEcC50 24-hrStaticLC50 24-hrStaticLC50 48-hrStaticLC50 48-hr StaticLC50 .48-hrFlow-throughLC50 2.9mg/L (EnviroSystems1991) 33.0mg/L (AQUIRE 2;1977) 7.2,10.0,& 12.0(AQUIRE 3;1983) 7.02.1mg/L (avgof8;AQUIRE 2; 1982) 5.00.5mg/L (avg of3;AQUIRE 3; 1983) 1.8mg/L (AQUIRE 2;1981) FatheadMinnow (Pimaphalespromelas) 24-hrStatiLcCso 48-hrStaticLC50 96-hrStaticLC50 7.4& 8.5mg/L (AQUIRE 3;1983) 6.6& 7.3mg/L (AQUIRE 3;1983) 6.6& 6.9mg/L (AQUIRE 3;1983) Bluegil(lLepomismacrochinis) 96-hrFlow-througLhC50 4.5mg/L (AQUIRE 2;1981) Mummichog (Fundulusheteroditus) 96-hrStatiLcC50 2.1mg/L (AQUIRE 2) Sheepshead Minnow (Cypdnodonvatiegatus) 24-hrStatiLcC50 10.0mg/L (AQU IRE 2) 48-hrStaticLC50 9.0mg/L (AQUIRE 2) 96-hr StaticLC50 9.0mg/L (AQUIRE 2) High-eyesMedaka (Oryzialsadpes) 6-hrRenewal LC50 24-hrRenewal LC5. 48-hrRenewal LC50 67 mg/L (AQUIRE 2) 46 mg/L (AQU IRE 2) 46 mg/L (AQUIRE 2) Bdne Shrimp(Artemiasalina) 24-hrStatiLcC50 3.6mg/L (AQUIRE 2;1974) AtlantiScilversid(eManidiamenidia) 96-hrStatiLcCo 2.8mg/L (AQUIRE 2;1982) Killifi(sFhundulussimilis) 24-hrStatiLcC50 48-hr StaticLCso 96-hrStaticLCso 4.7mg/L (AQUIRE 2) 4.7mg/L (AQUIRE 2) 4.5mg/L (AQUIRE 2) Rainbow Trout(Salmogairdnen) 48-hrFlow-througLhCso 96-hrFlow-throughLC50 10-day Flow-throughLC50 5.95mg/L (Verschuerenp 793;1977) 4.62mg/L (Verschuerenp 793;1977) 2.85mg/L (Verschuerenp 793;1977) .15- Zebrafi(sBhrachydanriaotio) 48-hrFlow-throuLgCh50 96-hr Flow-throughLCso lo-day Flow-throughLC50 8.81mg/L (Verschuerenp 793;1977) 7.97mg/L (Verschuerenp 793;1977) 7.97mg/L (Verschuerenp 793; 1977) Flagfis(hGordanellaflotidae) 48-hrFlow-throughLC50 96-hrFlow-throughLCso 10-day Flow-throughLC50 10.0mg/L (Verschuerenp 793; 1977) 8.10 mg/L (Verschuerenp 793; 1977) 6.90 mg/L (Verschuerenp 793; 1977) EnvironmentalAssessment: Literaturdeata indicatefsairllyow toxicittyo bacteriand althoughno actualBOD testdata are availablet,hismaterialshouldbe readildyegraded by microorganisms.A BOD value shouldbe obtainedby our own BOD tests. This materialismoderatelytoxictofish,Daphnia;and algae.Our most recentDaphnia test data agrees wellwithliteratudraeta which givesus confidenceinthe contractlaboratory's work on thismaterialB.ecause thereissufficienftishtoxicitdyata from the literaturneo,fish testingiswarranted. Because ofthealgaltoxicitsyhown inthe literatudraeta,algae testingshould be done. Even though thismaterialistoxictoa varietyofaquaticorganisms,itreadilydegrades and adverseeffectwsillbe minimalifitispassed througha wastewatertreatmentsystem orifa significanutmber ofmicroorganismsare presentina naturalwatertodegrade itrapidly. We shoulddo some studiesinwhich our productispasses througha model wastewater treatmentsystem and the measure thetoxiceffectsofthe model'seffluentA.notherstudy would be a simulationofa naturalbody ofwater usingan aquanum stockedwithfishT.his would giveus an understandingofthe degradationina riverp,ond,or lake. Recommendations forAdditionaTlesting: BOD, COD, and Microtox. 4.Tolyltriazol(eRM 27513; Corrosion InhibitorTolyltriazolTeechnical Grade) CAS # 29385-43-1 C7H7N3 N N N H Synonyms and vendors: Methyl-1H-Benzotriazole -16- Methylbenzotdazole CobratecTr-100 (PL4C,Inc.13) PreventolC17-100 (Mobay Corp."') DegradabiUW BOD Toxicijye Water Flea (Daphnia magna) 48-hr StaticEC50 BluegilSlunfish 96-hr LC50 Rainbow Trout 96-hr LCso Fathead Minnow .96-hrLCso Additionadlata fora similarcompound: 1H-Banzotdazole(C,,HN3) CAS Number: 95-14-7 Synonyms: 1,2,3-Benzotriazole Aziminobenzene Benzene Az7imide Benzotdazole Bluegilslunfish(Lepomis macrochirus) Static24-hrStress Rainbow Trout(Salmo gairdnen) Static24-hrStress Sea Lamprey (Patromyzonmadnus) Static24-hrStress Chinook Salmon (Oncorhynchus tshawytsqha) Static24-hrMortality <60% THOD (Mobay Corp.) 19 mg/L (EnviroSystems1991) 31.0mg/L (Mobay Corp.and PMC) . 21.4 mg/L (Mobay Corp.and PMC) 25.5mg/L (Mobay Corp.and PMC) o@/@ H 5 mg/L (AQUIRE 3;1957) 5 mg/L (AQUIRE 3;1957) 5 mg/L (AQUIRE 3;1957) 10 mg/L (AQU IRE 3;'1969) 13 PMC SpecialitieGsroup, Inc.TechnicalBulleti3n200.Contact:BilMlautlevAcz (513-242-3300). 14 Mobay Corp.data givenby Matthew Bartman 412-777-2000. -17- Coho Salmon(Oncorhynchuksisutch) Stati2c4-hrMortality 10 mg/L (AQU IRE 3;1969) NorthernSquawfish (Ptychocheiluosregonansis) Static24-hrMortality 10 mg/L (AQU IRE 3;1969) EnvironmentalAssessmento While the limitedata shows thatthismatedalismoderatelytoxic,itispresentatsuch low concentration(0.05%)inthe product,itmay have littelfefectinthe overalltoxicitoyfthe product.Because ofthe toxicitoyfthismaterialw,e need a complete setofdata.There is no reason to repeatthe fishdata,however. This materialislisteodn theTSCA 8(d)lisatnd any testingdone must be reportedtothe US EPA. Recommendations forAdditionaTlestinge BOD, COD, algae,and Microtox. 5. Foamer (FM-41IS) Toxicity: Water Flea (Daphnia magna) 48-hr StaticECSO 68 mg/L (EnviroSystems1991) EnvironmentalAssessment: The foamer shows slightoxicittyoDaphnia,butthereisno data forthe remaining environmentaleffectsW.e should have a complete setofeffectsdata forthismaterial. Care shouldbe takenthatthe sample used inthe environmentaleffecttsestingisagood representativseample which has been produced by the manufacturingmethod which will be used inthe future. Recommendations forAdditionaTlesting: BOD, COD, fish,ajgae,and Microtox. 6. Filmor (FM-3820 or FC-95) Degradabift BODs BOD,O BOD20 COD BOD/COD Toxicity: nil(PED) nil(PED) nil(PED) nil(PED) 0 -18- WaterFlea(Daphnimaagna) 48-hrStatiEcC50 48-hrStaticEC50 49 mg/L (EnviroSystems1991) 50 mg/L (PED) ActivatedSludge InhibitorCyonc. >4,000 mg/L (PED) FatheadMinnow (Pimephatespromelas) 96-hrLC50 38 mg/L (PED) BluegilSlunfish(Lepomismacrochinis) 96-hrLC50 68 mg/L (PED) Rainbow Trout(Salmogairdnen) 96-hrLC50 11 mg/L (PED) EnvironmentalAssessmento The filmerwillnotcontributteothe BOD/COD ratios,o toxicitiysthe issuewiththis component.This materialdoes show slightoxicittyofishand Daphnia.There isgood agreement between previousand presentDaphnia toxicitdyata which givesus confidence inthe most recentdata from EnviroSystems. We need to have a completesetofeffectsdatafora sample representativoefa recent manufacturinglotwhich has been produced by the manufacturingmethod which willbe used inthefuture. Recommendations forAdditionaTlesting: Algae and Microtox. 7.Na-octylsulfate(RM 32067 or RM 55040's) CAS # 142-31-4 Vendors*AlcolacIncorporated'(rSlodium OctylSulfateSolution)S;tepan Company '7 (POLYSTEP B-29). Degradabift THOD 1.88g/g(calculated) 15 Wim Vanneste'sPROFS note dated 03/12/1990givesthisRM#. 16 Alcolac:301-355-2600.No data available. 17 Called708-446-7500 ext.2283:they have no data. -19- BOD Swishee" reportsthat-lineaprrimaryalkylsulfateshave longbeen recognizedas extremelyrapidinprimarybiodegradationo,ftendisappearing inlessthan a day" and citesapproximately100 referenceswhich show degradationratesof80 to100% withina few hours ordays. Toxicijy:"' AustraliaBnarnacle(Eiminiusmodestus) 30-minStaticImmobilizatioEnC50 3,970 mg/L (AQUIRE 2;1976) Water Flea(Daphniamagna) 48-hr StaticEC50 517 mg/L (EnviroSystems1991) EnvironmentalAssessment: Althoughno BOD orCOD data are availablet,hismaterialwillmost likelyhave a high BOD/COD ratioB.OD and COD datashouldbe obtainedso thatwe have numericalvalues which have been obtainedintestscomparable tothose used forthe remaining components. There issurprisinglyittlieteratudraeta availabloen the aquatictoxicitoyfthissurfactant, however forthe Daphnia data itshows considerablylesstoxicittyhan the C,2surfactant. Thissurfactanitspresentat3% so itmay be a majorfactortotoxicitoyfthe product.Fish, algae,and Microtoxtoxicitdyataare essentiatlocompletethe assessment ofthis ingredient. Recommendations forAdditionaTlesting: BOD, COD, fish,algae,and Micmtox. 8.DI-water(RD 199) Toxicity: Water Flea (Daphnia magna) 48-hrStaticEC50 >1,000 mg/L (EnviroSystems1991) EnvironmentalAssessment: While itmay seem foolisthotestthe.deionizedwaterfortoxicittyo,xiceffectshave been tracedtocontaminateddeionizedwatersuppliesinthepast.The Daphnia resultindicates thatthesupplywas notcontaminatedand no furthetrestingofthe deionizedwatersample iswarranted. Recommendations forAdditionaTlesting: None. 18 Swisher,R. D. SurfactantBiodegradation2,nd ed. Marcel Dekker*New York,1987, p 466, 807-811. 19 There was 1 testgiveninthe EPA AQUIRE database. -20- 9.FC-203 DegradabihW BODR, BOD20 COD BOD5/COD Toxic'lyo Microtox ECIO EC50 ActivatedSludge Inhibition Algae (Chlorelipayrenoidosa andPhormidiuimnundatum) Algae(Scenedesmusubspicatus) 72-hrStatiPcopulG.rowthlnhibE.Clo 72-hrStaticPopul.Growth lnhib.EC50 Water Flea(Daphniamagna) 24-hrStaticEC,, 24-hrStaticEC5. 48-hr StaticEC50 48-hr StaticEC50 560 g/L(PED) 717 g/L(German Army Report2O) 1,060g/L(PED) 1,070g/L(PED) 1,373g/L (German Army Report) 0.52(PED and German Army Report) 632 mg/L (German Army Report) 2,500mg/L (German Army Report) none at1,000mg/L (PED) no effecatt:51,000mg/L (PED 21) 16.9mg/L (German Army Report) 160'mg/L (German Army Report) 31.6'mg/L(German Army Report) 430 mg/L (German Army Ri)port) 229 mg/L (EnviroSystems1991)22 1,600mg/L (PED 23) 20 "3% applicatiosnolutionsw"ere used toobtain'thBeOD ,and COD data inthe German Army Report.The German Army valueswere 41,200 mg/L and 21,500 mg/L forthe COD and BODE,,respectivelyT.he German Army valueswere multipliebdy 33.3 to getthevalues inthisreport. 21 Testingdone by IndustriaBlio-TestLaboratory,Jan. 1974. 22 EnviroSystems isrepeatingthistestbecause therewas notenough variatioinnthe FC-203 concentrationtshey used todevelopa good standardcurve. 23 Testingdone by Bionomics Laboratory,Dec. 1973. -21- EasternOyster Embryo-larvae (Crassostvrieraginica) 48-hSrtatEiCc50 47 mg/L (PED 24) GrassShrim(pPalaemoneptuegsio) 96-hSrtatLiCc5. 510 mg/L (PE D 25) Fatheamdinnow(Pimaphaplreosmelas) 96-hSrtatLiCc50 750 mg/L (PED) Zebrafish(Brachydanioretio) 48-hrStaticLCO 48-hr StaticLC50 1,053mg/L (German Army Report) 1,634mg/L (German Army Report) Scud (Gammarus fasciatus) 48-hrStaticLC50 1,100 mg/L (PED26) Rainbow trout(Oncorhyncus mykise) 96-hrStaticLC50 1,300mg/L (PED) Rainbow trout(Oncorhyncusmykiss) 96-hrContinuousFlow LC50 400 mg/L (PED28) Common mummichog (Fundulusheteroclitus) 2,500mgtl (PED29) 96-hrStaticLC50 Grass Shrimp (Palaemonetes pugio) 96-hr LC50 510 mg/L (PED) EnvironmentalAssessment: 1.The fishtoxicitdyata from the German Army Report agree'swellwithdata from previous 3M studies,so no fishtestingisneeded. 2.The Microtoxtestsfrom theGerman Army Report seem reasonable,so thistestShould notbe repeated. 3.The 24-hrEC50 Daphnia toxicitfyrom the most recentEnviroSystems work and the 48-hr EC50 from the German Army Reportare infairlgyood agreement. However, we weren't satisfiewdiththe concentrationussed inthe EnviroSystems work and they are repeating 24 Testingdone by Bionomics Laboratory,July1980. 25 Testingdone by Bionomics Laboratory,Aug. 1979. 26 Testingdone by Bionomics Laboratory,Dec. 1973. 27 FormerlySalmo gairdneti. 28 No mortalitoyrabnormal effectsatthisconcentration. 29 Testingdone by Bionomics Laboratory,Aug. 1979. -22- thiswork.The largedifferencebetween German Army valueand the 1973 PED value may be due toformulationchanges orchanges inthetoxicittyestprotocolbutthiscannot be verified. 4.As withthe foamer and filmerw,e need tobe carefulthatthesample submittedfor testingisofknown compositionand from a known manufacturinglot. 5.Sincetoxicittyo algaewas ofconcern intheGerman probablythe most important. Recommendations forAdditionaTlesting: BOD, COD, and algae. Army Report,the algae datais 10.VW1 60390 (FC-203 without TolyltriazolReM 27513) Toxicity: Water Flea (Daphnia magna) 48-hr StaticEC50 165 mg/L (EnviroSystems1991)30 EnvironmentalAssessmento 1.Sincethe onlydifferencebetween thismaterialand FC-203 istolyftriazotlees,tingthis materialalongwithFC-203 seems likea good way to.determinethe effectoftolyltdazole. For the recentDaphnia data,thereislittdliefferencebetween FC-203 and VW1 60390 and theeffectoftolyltdazolceannot be seen. Infact,thetoxicitoyfFC-203 was slightllyess than thetoxicitoyfVW1 60390 inthe recentEnviroSystemswork.Since EnviroSystemsis repeatingthe Daphnia forthese two samples,we willhave towaituntiElnviroSystems has completed thesetestsbeforemaking an evaluation. 2.A Micmtox and fishtoxicittyestshouldbe done on thismaterialand the results compared tothe FC-203 resulttsosee ifthereisa measurable effectcaused by tolylthazolienthe product. Recommendations forAdditionaTlesting: BOD, algae,fish,and Microtok.* 30 EnviroS*ystems isrepeatingthistestbecause therewas notenough variatioinnthe concentrationtshey used todevelopa good standardcurve. -23- 1.FC-203CE DegradabiUW BODs BODIO BOD20 COD 91 g/L(PED) 400 g/L(PED) 680 g/L(PED) 762 g/L(PED) BOD2dCOD ratio 0.89 (PED) TotalOrganicCarbon (TOC) 220 g/L (PED) OECD Method 301 E 88-90% biodegradability in14 days (PED) Toxicilys Algae (Selenastnimcapricomutum) 95-hr IC50 >1,000 mg/L (PED) OECD ActivatedSludge Respiration Inhib@itiToenst 209 none at 1,000 mg/L Water Flea (Daphnia magna) 48-hr StaticLC50 >1,000 mg/L (PED) - Microtox (Photobacterium phosphoreum) Exposure: 5 min. 15 min. 30 min EC50 370 mg/L 260 mg/L 230 mg/L Killifi(sFhundulusheteroclitus) 96-hrContinuousFlow L050 1,400mg/L (PED) FatheadMinnow (Pimephalespromelas) 96-hrContinuousFlow LC50 96-hrStaticLCso >2,000mg/L (PED) >1,000 mgA- (PED) EnvironmentalAssessment: The environmentaleffectsdataforFC-203CE isfairlcyomplete. The PED datashows lowertoxicittyo Daphnia than isshown by FC-203 inthe German Army Reportand from the recenttestsby EnviroSystems.Daphnia testingshould be done againforassurance inour PED. Recommendations forAdditionaTlesting: Algae and Daphnia. -24- 12.FC-206CE DegradabilZ BODs BOD,O BOD20 COD BOD2dCOD ratio TotalOrganicCarbon (TOC) OECD Method 301 E 46 g/L(PED) 260 g/L(PED) 470 g/L(PED) 402 g/L(PED) 1.2(PED) 130 g/L(PED) 89-92% biodegradability in14 days (PED) Toxicity: Algae (Selenastrumcapricomutum) 95-hr IC50 OECD ActivatedSludge Respiration InhibitioTnest 209 Water Flea (Daphnia magna) 48-hr StaticLC50 >1,000 mg/L (PED) none at1,000mg/L >1,000 mg/L (PED) Microtox(Photobacteriumphosphoreum) Exposure: 5 min. 15 min. 30 min Killifi(sFhundulus hateroclitus) 96-hrContinuous Flow LC,, EC50 650 mg/L 4500. mgA4200 mgAL >2,000mg/L (PED) FatheadMinnow (Pimephalespromelas) 96-hrContinuousFlow LC50 96-hr StaticLC50 >2,000mg/L (PED) >1,000 mg/L (PED) EnvironmentalAssessment: The environmentaleffectsdata forFC-203CE isfairlcyomplete. Again,the PED data shows lowtoxicittyoDaphnia which shouldbe verified. Recommendations forAdditionaTlesting: Algae and Daphnia. -25- 13.FC-203CF EnvironmentAaslsessmento No environmentaleffectsdata forthisproductare availablaend a complete setof environmentaleffectsdata shouldbe obtained. Recommendations forAdditionaTlesting: BOD, COD, algae,Daphnia,fish,and Microtox. 14.FC-206CF EnvironmentalAssessment: No environmentaleffectsdata forthisproductare availablaend a complete setof environmentaleffectsdata shouldbe obtained. Recommendations forAdditionalTesting@ BOD, COD, algae,Daphnia, fish,and Microtox. 15.L-4640 (New Foamer) Testingiscurrentlyunderway fora European PMN by NOTOX Laboratories: 1.Daphnia toxicitsytudy (C2 EEC testmethod). 2. Fishtoxicit(yCl method) usingZebra fish(Brachydanioretio). 3. ModifiedSturm test(C5)forreadybiodegradability. Alltestingforthe European PMN isbeingdone by GLP. The followingenvironmentaleffectsdata arefrom several3M technicalreportswhich are compiled inthe report"EnvironmentalData and InformatioinnSupportofthe PMN for Certain-ChemicalSubstances Designatedas L-4640",June'20,1880. DegradabigW BODs BODIO BOD20 COD TheoreticalOxygen Demand Toxicity: Acute Effecton MicrobialRespiration Water Flea (Daphnia magna) 48-hr StaticLCSO Fathead Minnow (Pimephales promelas) 96-hr StaticLCso <0.038 g/g <0.038 g/g <0.038 g/g 0.41 g/g 0.75 g/g 8% reductioninrateofoxygen depletionat 1,000mg/L 100 mg/L and 111 mg/L 158 mg/L and 166 mg/L -26- EnvironmentalAssessment: Because ofthe importanceofthismaterialtofutureproducts,a fulsletofenvironmental effectsdata isneeded. The environmentaleffectsdata notincludedinthe PMN work or in the previousstudiesshould be obtained:algae and Microtox. Recommendations forAdditionaTlesting@ Algae and Micmtox. 16.Witcolate7093 (RM 45666) CAS # 73665-23-2 Witeo Corporation(713-433-7281)has no ecotoxinformationavailableW.itcblate7093 is 55% water,45% C6-C 10 ethoxylatedsulfateT.hey recommended Swisherfor biodegradationinformation. No informationon EPA AQUIRE database nor inVerschueren. EnvironmentalAssessment: No effectsdata isavailableT.estingdepends on the likelihootdhatthismaterialwillbe used infutureproducts. Recommendations forAdditionaTlesting: COD, BOD, algae,fish,Daphnia,and Microtox. 17.Triethanolamine CAS # 102-71-6 DegradabiW' THOD BOD5 BODIO BOD,s BOD20 2.04 g/g nil 0.02 g/g withstandardseed 0.17 g/g adapted sewage 0.03 g/g 0.02 g/g nilwithstd.dilutedsewage 0.05 g/g 0.13 g/g 31 Alldegradabilitdyata are from Verschueren p 1150-1. -27- COD BODs/COD BOD20/COD Toxicity2: Bacteria(Pseudomonas putida) Popul.Growth Inhib. Algae (Microcystfaseruginosa) Popul.Growth Inhib. Green Algae (Scenedesmus quaddcalda) 7-dayStaticPopul.Growth lnhib. 8-day StaticPopul.Growth lnhib. 8-day StaticPopul.Growth lnhib. Blue-greenAlgae (Anacystisaeruginosa) 8-dayStaticPopul.Growth lnhib. Protozoa(Entosiphonsulcatum) 72-hrStaticPopulationGrowth Protozoa(Chilomonasparamecium) 48-hrRenewal Popul.Growth Inhib. Protozoa(Uronema pardurzi) Bacteria(Pseudomonas) LCO Algae (Scenedesmus) Leo Algae (Colpoda) LCO Water Flea(Daphnia) LCO 24-hrStaticLC50 BrineShrimp (Attemiasalina) 24-hrStaticLC50 1.50g/g nilto 0.11 0.09 >10,000 mg/L (Verschuerenp 1151) 47 mg/L (Verschuerenp 115 1) 1.8mg/L (AGUIRE 2;1980) 1.8mg/L (AQUIRE 2;1078) 715 mg/L (AQUIRE 2;1978)33 19 mg/L and 47 mg/L (AQUIRE 2;1978) 56 mg/L (AQUIRE 2;1980) 1,768mg/L (AQUIRE 4; 1980) >10,000mg/L (Verschuerenp 1151) 10,000mg/L (Verschuerenp 1151) 100 mg/L (Verschuerenp 1151) 160 mg/L (Verschuerenp 1151) 2,500mg/L (Verschuerenp 1151) 1,390mg/L (AQUIRE 2; 1977) 5,600 mg/L (AQUIRE 2; 1974) 32 The toxicitdyata are a partiallistinogfthe 16 testson 10 speciesgiveninthe AQUIRE database. 33 Thisvalueisfrom a differenrteferencethan the previousone. -28- Goldfis(hCarassiauusratus) 24-hrStatiLcC50 24-hrLC50(85% TEA) 5,000mg/L (AQUIRE 2; 1979) 75,000 mg/L atpH 7 3,500 mg/L atpH 10.3 (Verschuerenp 1151) EnvironmentalAssessment: This materialshows insignificatnotxicittyoDaphnia and fish. However, ithas a low BOD/COD ratioand willcontributteoa low ratiointhe product.In additioni,tshows considerabletoxicittyoalgae.For these reasons,itwould be best notto use thismaterialinproductsunlessno alternativcean be found or itsconcentrationinthe productwillbe extremelylow. The literatudraeta isessentiallcyompleteforthismaterialA.ny testingon thismaterialmust be reportedto US EPA under sectionTSCA 8(d). Recommendations forAdditionaTlesting: None. 18.Urea CAS # 57-13-6 Degradabigbt@4 Degrades abiotically: H2NCONH2+ H20 ---> C02 Deradationrateat20*C + NH3 Degradationrateat2*C Concentrationindomesticsewage 10.9mg/ljhr 3.3mg/Uhr 2 to6 mg/L Bacteria(Psoudomonas putida) Popul.Growth Inhib. Green Algae (Scenedesmus quadricauda) 7-day StaticPopul.Growth lnhib. 8-day StaticPopul.Growth Inhib. >10,000 mg/L (Verschuerenp 1178) 10,000mg/L (AQUIRE 2; 1980) 10,000mg/L (AQUIRE 2; 1978) 34 Alldegradabilitdyata are from Verschueren p 1178-9. 35 The toxicitdyata are a partiallistinogf32 testson 17 speciesfrom the EPA AQUIRE database. -29- Blue-greAelngae(Anacystaiesruginosa) 8-dayStatiPcopulG.rowthlnhib. 47 mg/L (AQUIRE 2; 1978) Protozoa(Entosiphonsulcatum) 72-hrStaticPopul.Growth lnhib. 29 mg/L (AQUIRE 2;1980) Protozoa(Chilomonasparamecium) 48-hrPopul.Growth Inhib. 2,683mg/L (AQUIRE 4; 1980) Water Flea(Daphnia) 24-hrStaticLC50 10,000 mg/L (AQUIRE 2;1977) Creek Chub (Semotilusatromaculatus) 24-hrLCO 24-hr LCIOO 16,000mg/L (AQUIRE 2;1952) 30,000mg/L (AQUIRE 2;1952) EnvironmentalAssessment* Literaturdeata indicatesthatthismaterialshows insignficanttoxicittyo DaphniA fisha,nd green algae.Furthermore,itdegrades readilyand itmost likelwyould notbe a problemin the product. However, itdoes show toxicittyoblue-greenalgae,and the low toxicittyogreen algae should be verifiebdy ourown testsifitistobe used inthe productathighconcentrations. Recommendations forAdditionaTlesting: None. 19. Triton X-305 CAS # 9002-93-1 (PRISM shows Tdton X-305 to be 70% thismaterialwith the -compositionofthe remaining30% notgiven). Synonyms: Poly(oxy-1,2-ethanediyal)p,ha-[4-(1,1,3,3-tetramethylbutyl)phenyl]omega-hydroxy-polyethylengelycolp-1,1,3,3-tetramethylbutylphetnhyelr Octoxinol P-tert-octylphenoxypolyethoxyethanol Vendor: Rohm & Haas Company3l 36 Independence MailWest, PhiladelphiaP,A 19105. Phone: 215-592-3000. -30- 37 Bluegil(lLepomis macrochinis) 24-hr StaticLCro 24-hrStaticLC, 24-hr StaticLC50 24-hr Flow-throughLC5. 96-hrStaticLC50 96-hrStaticLC50 96-hrStaticLCso 96-hrFlow-throughLC5. 6-day Flow-throughLC, 3.5mg/L (AQUIRE 1;1975) 16.2mg/L (AQUIRE 1;1975) 1,080mg/L (AQUIRE 1;1975) 10.0mg/L (AQUIRE 2;1975) 2.8mg/L (AQUIRE 1;1975) 12.Omg/L (AQUIRE 1;1975) 531 mg/L (AQUIRE 1;1975) 1O.Omg/L (AQU IRE 2; 1975) 9.6mg/L (AQUIRE 2;1975) The reason forthe highvaluesforthe 24-hrStaticLC50 (1,080mg/L) and forthe 96-hr StaticLC5. (531 mg/L )are notknown. The vendor forTritonX-305 (Rohm and Haas) has no data on the aquatictoxicitoyr biodegradabiliotfyTritonX-305. However, they are sendinga datasheet on the aquatic toxicitoyfTritonX-100.TritonX-100 has a lowermolecularweightthan X-305 and they believethatthe toxicitoyfX-305 islessthan X-100 because X-305 islesssurfaceactive. TritonX-305 isnotbelievedtodegrade biologicallRyo.hm and Haas has recentlysoldtheir Tritonproductlineto Union Carbide (1-800-752-1827)and willbe transferrintghe business in-thenear futureT.he CAS number givenby Rohm and Haas fortheseriesis9036-19-5. The followingdata isfrom AQUIRE on thisCAS number. CAS 9036-19-5 Rainbow Trout(Salmogairdnen) 48-hrStatiLcC50 96-hr StaticLC5. 7.2mg/L (AQUIRE 2; 1978) 7.2mg/L (AQUIRE 2;1978) MarinePolychaeteWorm (Scolelepifsuliginosa) 48-hrStaticLC50 96-hr StaticLC50 17.0mg/L (avg.of7 points) 13.7mg/L (avg.of8 points) (AQUIRE 2;1978) 37 The toxicitdyata are a partiallistinogf 13 testson 5 speciesfrom the EPA AQUIRE database. 38 The toxicitdyata are a partiallistinogf36 testson 5 speciesfrom the EPA AQUIRE database. -31- MarinePolychaetWeorm (Capitelciaapitata) 48-hrStatiLcCw 96-hrStaticLCso 8.2mg/L (avg.of6 points) 6.0mg/L (avg.of8 points) (AQUIRE 2;1978) AquaticSowbug (idoteabafthica) 48-hrRenewal LCso 96-hr Renewal LC50 10.0mg/L (AQUIRE 3; 1972) 5.0 mg/L (AQUIRE 3;1972) EnvironmentalAssessment: Both CAS numbers have a limiteadmount oftoxicitdyata availablwehich shows modertite toxicitSyi.nce Tdton X-305 isa majorcomponent ofthe CE products,we willneed a completesetofdata on thismaterialinorderinordertocorrelattehe effects,otfhe CE productstotheirformulation. Recommendations forAdditionalTestingm BOD, COD, algae,DaphniA fish,and Microtox. -32-